| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| Carbonic anhydrase 2 ( CA 2 ) was expressed at higher abundance in the surface mucous cells and mucous neck cells , which expressed NHE 1 , than in the parietal cells , which expressed AE 2 . ^^^ The morphological evidence identified AE 2 as a major parietal cell anion exchanger , whereas NHE 1 and CA 2 colocalized in mucous neck , chief , and surface mucous cells . ^^^ Carbonic anhydrase 2 ( CA 2 ) was expressed at higher abundance in the surface mucous cells and mucous neck cells , which expressed NHE 1 , than in the parietal cells , which expressed AE 2 . ^^^ |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| Using a microtiter plate binding assay we showed that the C terminal region of NHE 1 binds carbonic anhydrase 2 ( CAII ) and binding was stimulated by low pH and blocked by antibodies against the C terminal of NHE 1 . ^^^ The binding to NHE 1 was confirmed by demonstrating protein protein interaction using affinity blotting with CAII and immobilized NHE 1 fusion proteins . ^^^ CAII co immunoprecipitated with NHE 1 from CHO cells suggesting the proteins form a complex in vivo . ^^^ In cells expressing CAII and NHE 1 , the H ( + ) transport rate was almost 2 fold greater than in cells expressing NHE 1 alone . ^^^ The CAII inhibitor acetazolamide significantly decreased the H ( + ) transport rate of NHE 1 and transfection with a dominant negative CAII inhibited NHE 1 activity . ^^^ |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| It is concluded that chloride enters the basolateral aspect of the cells using the Na+ K+ 2Cl cotransporter and a parallel arrangement of NHE 1 with AE 2 , these latter two being sensitive to acetazolamide because of their association with the cytoplasmic form of carbonic anhydrase CAII . ^^^ |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| Carbonic anhydrase 2 ( CAII ) binds to and regulates transport by the NHE 1 isoform of the mammalian Na ( + ) / H ( + ) exchanger . ^^^ CAII did not bind to acidic sequences in NHE 1 that were similar to the CAII binding site of bicarbonate transporters . ^^^ Phosphorylation of the C terminal 26 amino acids by heart cell extracts did not alter CAII binding to this region , but phosphorylation greatly increased CAII binding to a protein containing the C terminal 182 amino acids of NHE 1 . ^^^ The results demonstrate that a novel phosphorylation regulated CAII binding site exists in distal amino acids of the NHE 1 tail . . ^^^ A novel carbonic anhydrase 2 binding site regulates NHE 1 activity . ^^^ |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| Bicarbonate secretion by rat bile duct brush cells indicated by immunohistochemical localization of CFTR , anion exchanger AE 2 , Na+ / HCO3 cotransporter , carbonic anhydrase 2 , Na+ / H+ exchangers NHE 1 and NHE 3 , H+ / K+ ATPase , and Na+ / K+ ATPase . ^^^ |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P19634 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|