| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Cells positive for AE 1 and H ( + ) ATPase were common in all collecting duct regions in normal mice but were virtually absent from the inner stripe of the outer medulla and the inner medulla of CAR 2 null mice . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| The human Cl ( ) / HCO ( 3 ) ( ) anion exchanger ( AE 1 ) possesses a binding site within its 33 residue carboxyl terminal region ( Ct ) for carbonic anhydrase 2 ( CAII ) . ^^^ A series of truncation mutants of the GST Ct showed that the terminal 21 residues of AE 1 were not required for binding CAII . ^^^ A GST fusion protein of the 33 residue Ct of AE 2 could bind to CAII similarly to the Ct of AE 1 . ^^^ Identification of the carbonic anhydrase 2 binding site in the Cl ( ) / HCO ( 3 ) ( ) anion exchanger AE 1 . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Mutations in the gene SLC4A4 , encoding Na+ HCO 3 cotransporter ( NBC 1 ) , have been found in proximal RTA with ocular abnormalities ; in the gene SLC4A1 , encoding Cl ( ) HCO 3 exchanger ( AE 1 ) , in autosomal dominant distal RTA ; in the gene ATP6B1 , encoding B 1 subunit of H+ ATPase , in autosomal recessive distal RTA with sensorineural deafness ; and in the gene CA 2 , encoding carbonic anhydrase 2 , in autosomal recessive osteopetrosis . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Human carbonic anhydrase 2 ( CAII ) possesses a binding site for an acidic motif ( D887ADD ) within the carboxyl terminal region ( Ct ) of the human erythrocyte chloride / bicarbonate anion exchanger , AE 1 . ^^^ In this study , the amino acid sequence comprising this AE 1 binding site was localized to the first 17 residues of CAII , which form a basic patch on the surface of the protein . ^^^ Truncation of the amino terminal of CAII by five residues resulted in a 3 fold reduction in the apparent affinity of the interaction with a GST fusion protein of the Ct of AE 1 ( GST Ct ) measured by a sensitive microtiter plate binding assay . ^^^ The homologous isoform CAI does not bind AE 1 , despite having 60 % sequence identity to CAII . ^^^ These results indicate that the AE 1 binding site is located within the first 17 residues of CAII , and that the interaction is mediated by electrostatic interactions involving histidine and / or lysine residues . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical colocalization of HKalpha ( 2c ) with carbonic anhydrase 2 , the Cl ( ) / HCO exchanger AE 1 , and HKalpha ( 1 ) indicated that both type A and type B intercalated cells possessed intense apical HKalpha ( 2c ) immunoreactivity , whereas principal cells and connecting segment cells had only a thin apical band of HKalpha ( 2c ) . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| The cytoplasmic carboxyl terminal domain of AE 1 , the plasma membrane chloride / bicarbonate exchanger of erythrocytes , contains a binding site for carbonic anhydrase 2 ( CAII ) . ^^^ To examine the physiological role of the AE1 / CAII interaction , anion exchange activity of transfected HEK 293 cells was monitored by following the changes in intracellular pH associated with AE 1 mediated bicarbonate transport . ^^^ AE 1 mediated chloride / bicarbonate exchange was reduced 50 60 % by inhibition of endogenous carbonic anhydrase with acetazolamide , which indicates that CAII activity is required for full anion transport activity . ^^^ AE 1 mutants , unable to bind CAII , had significantly lower transport activity than wild type AE 1 ( 10 % of wild type activity ) , suggesting that a direct interaction was required . ^^^ To determine the effect of displacement of endogenous wild type CAII from its binding site on AE 1 , AE 1 transfected HEK 293 cells were co transfected with cDNA for a functionally inactive CAII mutant , V143Y . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Recently it was shown that AE 1 , found in erythrocytes and kidney , binds carbonic anhydrase 2 ( CAII ) via the cytosolic C terminal tail of AE 1 . ^^^ To examine the physiological consequences of the interaction between CAII and AE 1 , we characterized Cl ( ) / HCO ( 3 ) ( ) exchange activity in transfected HEK 293 cells . ^^^ Treatment of AE 1 transfected cells with acetazolamide , a CAII inhibitor , almost fully inhibited anion exchange activity , indicating that endogenous CAII activity is essential for transport . ^^^ Further experiments to examine the role of the AE1 / CAII interaction will include measurements of the transport activity of AE 1 following mutation of the CAII binding site . ^^^ Since over expression of V143Y CAII would displace endogenous wild type CAII from AE 1 , a loss of transport activity would be observed if binding to the AE 1 C terminus is required for transport . . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Expression of the inactive CAII V143Y mutant blocked the interaction between endogenous cytosolic CAII and AE 1 , AE 2 , and AE 3 and inhibited their transport activity ( 53 + / 3 , 49 + / 10 , and 35 + / 1 % inhibition , respectively ) . ^^^ However , in the presence of V143Y CAII , expression of CAIV restored full functional activity to AE 1 , AE 2 , and AE 3 ( AE 1 , 101 + / 3 ; AE 2 , 85 + / 5 ; AE 3 , 108 + / 1 % ) . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Compared with AE 1 , the COOH terminal tail of DRA interacted weakly with CAII . ^^^ Overexpression of a functionally inactive CAII mutant , V143Y , reduced AE 1 transport activity by 61 + / 4 % without effect on DRA transport activity ( 105 + / 7 % transport activity relative to DRA alone ) . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Colocalization of RhBG with carbonic anhydrase 2 , the thiazide sensitive transporter , and the anion exchangers AE 1 and pendrin demonstrated RhBG immunoreactivity in all CNT cells and all CCD and ICT principal cells . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| A homologous motif ( D887ADD ) in the carboxy terminus of the anion exchanger AE 1 binds to carbonic anhydrase 2 ( CAII ) . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Cl / HCO3 exchange activity mediated by the AE 1 anion exchanger is reduced by carbonic anhydrase 2 ( CA 2 ) inhibition or by prevention of CA 2 binding to the AE 1 C terminal cytoplasmic tail . ^^^ To test the hypothesis that CA 2 binding might itself allosterically activate AE 1 in Xenopus oocytes , we compared Cl / Cl and Cl / HCO3 exchange activities of AE 1 polypeptides with truncation and missense mutations in the C terminal tail . ^^^ In contrast , AE 1 896X , 891X , and AE 1 missense mutants in the CA 2 binding site were inactive as Cl / HCO3 exchangers despite exhibiting normal Cl / Cl exchange activities . ^^^ Co expression of CA 2 enhanced wild type AE 1 mediated Cl / HCO3 exchange , but not Cl / Cl exchange . ^^^ CA 2 co expression could not rescue Cl / HCO3 exchange activity in AE 1 mutants selectively impaired in Cl / HCO3 exchange . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Double immunostaining indicated that the AE 1 positive cells in the chorionic and allantoic epithelia were also positive for the carbonic anhydrase isoform , CAII , which serves as a marker for the villus cavity ( VC ) cells of the chorionic epithelium and the mitochondria rich cells of the allantoic epithelium . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Physical interactions have been identified between the carbonic anhydrase isoform , CAII , and the erythrocyte membrane Cl / HCO3 ( ) anion exchanger , AE 1 , mediated by an acidic motif in the AE 1 C terminus . ^^^ We have found that the presence of CAII attached to AE 1 accelerates AE 1 HCO3 ( ) transport activity , as AE 1 moves bicarbonate either into or out of the cell . ^^^ In efflux mode the presence of CAII attached to AE 1 will increase the local concentration of bicarbonate at the AE 1 transport site . ^^^ As bicarbonate is transported into the cell by AE 1 , the presence of CAII on the cytosolic surface accelerates transport by consumption of bicarbonate , thereby maximizing the transmembrane bicarbonate concentration gradient experienced by the AE 1 molecule . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| To characterize the location of glycolytic enzymes ( GEs ) in intact human erythrocytes , freshly drawn blood was fixed and stained with Abs to GAPDH , aldolase , phosphofructokinase ( PFK ) , pyruvate kinase ( PK ) , lactate dehydrogenase ( LDH ) , carbonic anhydrase 2 , Hb , and band 3 ( AE 1 ) . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| In the Xenopus oocyte , the AE 1 C terminal cytoplasmic tail residues reported to bind carbonic anhydrase 2 are dispensable for Cl Cl exchange , but required for Cl HCO 3 exchange . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| Others report that carbonic anhydrase 2 ( CA 2 ) binds to the C termini of the anion exchanger AE 1 and the electrogenic Na / HCO3 cotransporter NBCe 1 A , enhancing transport . ^^^ |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02730 and P00918 |
Pubmed |
SVM Score :0.0 |
| NA |
|