| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| After binding , pro uPA is activated by serine proteases ( e . g . plasmin , trypsin or plasma kallikrein ) and by the cysteine proteases cathepsin B or L , resp . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Different pathways of activation of pro uPA in tumor tissues may coexist : ( 1 ) autocatalytic intrinsic activation of pro uPA ; ( 2 ) activation by serine proteases ( plasmin , kallikrein , Factor XIIa ) ; and ( 3 ) activation by cysteine proteases ( cathepsin B and L ) . . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| HOC 1 ovarian cancer cells express the single chain form of the urokinase type plasminogen activator ( uPA ) and cathepsin B ( cath B ) on their cell surface . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Some proteases ( plasmin , cathepsin B and L , kallikrein , trypsin or thermolysin ) activate pro uPA by cleaving the peptide bond Lys 158 and IIe 159 . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B efficiently activates the soluble and the tumor cell receptor bound form of the proenzyme urokinase type plasminogen activator ( Pro uPA ) . ^^^ Action of purified human cathepsin B on recombinant single chain urokinase type plasminogen activator ( pro uPA ) generated enzymatically active two chain uPA ( HMW uPA ) , which was indistinguishable by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot from plasmin generated HMW uPA and from elastase or thrombin generated inactive two chain urokinase type plasminogen activator . ^^^ Preincubation of cathepsin B with E 64 ( transepoxysuccinyl L leucylamino ( 4 guanidino ) butane , a potent inhibitor for cathepsin B ) prior to the addition of pro uPA prevented the activation of pro uPA . ^^^ The cleavage site within the cathepsin B treated urokinase type plasminogen activator ( uPA ) molecule , determined by N terminal amino acid sequence analysis , is located between Lys 158 and Ile 159 . ^^^ Pro uPA is cleaved by cathepsin B at the same peptide bond that is cleaved by plasmin or kallikrein . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| The present study was undertaken to assess the role of membrane associated cathepsin B as an activator of receptor bound single chain urokinase type plasminogen activator ( pro uPA ) and to determine the importance of receptor bound uPA activity in the destruction of extracellular matrix by tumor cells with subsequent invasion through basement membranes . ^^^ Ovarian cancer HOC 1 cells express pro uPA / HMW uPA and cathepsin B on their surface . uPAs are bound to a specific surface receptor , about 30 % of which is saturated . 60 % of the receptor bound uPA is pro uPA . ^^^ Inhibition of cell surface cathepsin B activity was associated with a decrease in cell bound uPA activity to undetectable levels , and > 95 % of the membrane associated uPA was pro uPA in cells cultivated with E 64 . ^^^ This suggested that receptor bound pro uPA can not be converted to HMW uPA in the absence of enzymatically active cathepsin B . ^^^ These data support our hypothesis that membrane associated cathepsin B may be important for the conversion of pro uPA to HMW uPA and that receptor bound uPA activity constitutes an efficient mechanism which contributes to tumor cell invasion . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Urokinase type plasminogen activator ( uPA ) , uPA receptors , and cathepsin B were quantitated by using an immunological method , enzyme linked immunosorbent assay , and amidolytic activity assays in 15 malignant and 10 benign epithelial ovarian tumors . ^^^ The levels of uPA and uPA receptors , as well as cathepsin B , were found to be higher in membrane preparations obtained from malignant tumors than in those obtained from benign tumors . ^^^ In the membranes of malignant tumors , levels of uPA receptor and cathepsin B did not vary with stage of disease . ^^^ Since ovarian cancer cells produce both pro uPA and cathepsin B , the possibility of activation of tumor cell derived pro uPA by cellular protease cathepsin B must be considered . . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cysteine proteases ( cathepsin B [ CATB ] and cathepsin L [ CATL ] ) , the serine protease urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) are thought to play an important part in cancer invasion and metastasis . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| At both levels , concentrations of uPA were significantly correlated with those for uPAR . uPA levels also correlated significantly with cathepsin B and cathepsin D but not with cathepsin L , MMP 8 or MMP 9 levels . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Cysteine proteases [ cathepsin B ( CATB ) , cathepsin L ( CATL ) ] , the serine protease urokinase type plasminogen activator ( UPA ) and its inhibitor type 1 ( PAI 1 ) play an important part in cancer invasion . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Although the prognostic implications of the uPA and MMPs still remain unclear , cathepsin B appears to be one of the most useful prognostic markers so far reported for non small cell lung cancer . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cysteine proteases [ Cathepsin B and L ( CATB , CATL ) ] and the serine protease urokinase type plasminogen activator ( UPA ) with its inhibitor type 1 ( PAI 1 ) are thought to play an important part in colorectal cancer invasion and metastasis . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Cathepsin B ( CATB ) and cathepsin L ( CATL ) , which are cysteine proteases , urokinase ( UPA ) and tissue type plasminogen activator ( TPA ) , both serine proteases , and their inhibitor type 1 ( PAI 1 ) are believed to play an important role in colorectal carcinoma ( CRC ) invasion and metastasis . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Our purpose was to analyze the predictive relevance of DNA ploidy , S phase fraction ( SPF ) , and tissue levels of lysosomal proteinases cathepsin D ( CD ) , cathepsin B ( CB ) , cathepsin L ( CL ) , and urokinase type plasminogen activator ( uPA ) and that of the intracellular cysteine proteinase inhibitor stefin A on clinical outcome . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| The plasma levels of urokinase type plasminogen activator ( UPA ) and the serum levels of cathepsin B were significantly increased in patients with gastrointestinal tumours . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B can degrade extracellular matrix proteins , such as collagen 4 and laminin , and can activate the precursor form of urokinase plasminogen activator ( uPA ) , perhaps thereby initiating an extracellular proteolytic cascade . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Antigen levels of uPA and PAI 1 were determined by ELISA in detergent extracts ; cathepsin B , D , and L content was determined in cytosol fractions of the primary tumor : cathepsin D by ELSA and cathepsin B and L by ELISA . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Pro uPA activation was preceded by dramatic changes in lysosome trafficking and the extracellular appearance of cathepsin B and beta hexosaminidase but not cathepsins D or L . ^^^ Co treatment of cultures with the cathepsin B inhibitors CA 074 or Z FA FMK suppressed the cytostatic effects of TPA and activation of pro uPA . ^^^ In the absence of TPA , exogenously added cathepsin B activated pro uPA and suppressed MCF10A Neo proliferation . ^^^ Pretreatment with cycloheximide did not suppress the exocytosis of cathepsin B or the activation of pro uPA . ^^^ Subsequently , extracellular cathepsin B initiates a proteolytic cascade involving uPA , plasminogen , and plasmin that activates serum derived latent TGF beta . . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| In vitro chemoinvasiveness and gene expression of proteases including uPA , MMP 2 , 9 , MT 1 MMP , and cathepsin B , D , L , were not different between OPN transfected and control cells determined with matrigel invasion assay and cDNA expression macroarray , respectively . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| By using immunohistochemical techniques , we studied the expression of matrix metalloproteinase ( MMP ) 1 , MMP 2 , MMP 9 , cathepsin B ( CatB ) , and urokinase plasminogen activator ( uPA ) in 29 NSBCs and compared these data with clinicopathologic parameters and the expression of cell differentiation markers . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Presumable order of events in this complicated cascade is that aspartyl protease ( cathepsin D ) activates cysteine proteases ( e . g . cathepsin B ) that can activate pro uPA . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B and pro urokinase plasminogen activator ( pro uPA ) localize to the caveolae of HCT 116 human colorectal carcinoma cells , an association mediated by active K RAS . ^^^ In this study , we established a stable HCT 116 cell line with a gene encoding antisense caveolin 1 ( AS cav 1 ) to examine the effects of caveolin 1 , the main structural protein of caveolae , on the expression and localization of cathepsin B and pro uPA , and their cell surface receptors p 11 and uPA receptor ( uPAR ) , respectively . ^^^ Localization of cathepsin B and pro uPA to caveolae was reduced in AS cav 1 HCT 116 cells , and these cells expressed less total and caveolae associated p 11 and uPAR compared with control cells . ^^^ Based on these results , we hypothesize that caveolin 1 affects the expression and localization of cathepsin B and pro uPA , and their receptors , thereby mediating cell surface proteolytic events associated with invasion of colon cancer cells . . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| We found that purified enzymes such as trypsin , cathepsin B , uPA and type 4 collagenase suppressed the proliferative response in a dose dependent fashion . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Downregulation of 2 molecules , such as uPA and uPAR , uPAR and MMP 9 , or Cathepsin B and MMP 9 , are more effective to inhibit angiogenesis rather than downregulation of single molecules . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The effects of the bisphosphonate derivative zoledronic acid ( ZA ) on the circulating levels of matrix metalloproteinase 2 ( MMP 2 ) , matrix metallo proteinases 9 ( MMP 9 ) , cathepsin B ( Cath B ) and urokinase type plasminogen activator ( uPA ) in patients with bone metastasis ( BMTS ) and the possible correlation with the symptomatic response induced by this drug in these patients were evaluated . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| This enzyme was apparently different from urokinase type plasminogen activator or cathepsin B and was present in mammary tumour at levels at least 20 times higher than those in normal mammary tissue . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Other factors examined ( including the laminin receptor , cathepsin B , cathepsin L , urokinase type plasminogen activator , and tissue inhibitor metalloproteinases ) are expressed by both invasive and noninvasive gastric cancer cells , whereas collagenase type 4 92 kD form is not expressed by any of the cells examined . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| In vitro activation of pro cathepsin B by three serine proteinases : leucocyte elastase , cathepsin G , and the urokinase type plasminogen activator . ^^^ The urokinase type plasminogen activator activated pro cathepsin B faster than leucocyte proteinases . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cysteine proteases ( cathepsin B and L ) , the serine protease urokinase type plasminogen activator and its inhibitor type 1 play an important part in cancer invasion and metastasis . ^^^ The Cox survival analysis showed that cathepsin B ( p = 0 . 002 ) , urokinase type plasminogen activator ( p = 0 . 0001 ) and the inhibitor type 1 ( p = 0 . 0004 ) significantly correlated with poor prognosis . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Higher levels of urokinase type plasminogen activator , cathepsin B , and type 1 inhibitor were observed in Helicobacter pylori positive patients . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B is able to directly facilitate invasion by degrading extracellular matrix components or to indirectly facilitate invasion by activating other matrix degrading proteases like the urokinase type plasminogen activator . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Some of these genes were already known to be associated with apoptosis ( BIK , TEGT , SSI 3 ) , hypoxia inducible genes ( HIF1A , CA 12 ) , and tumor cell invasion and metastasis ( CTSL , CTSB , PLAU , CD 44 ) . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| METHODS : The expression patterns of urokinasase plasminogen activator ( u PA ) , its cell surface receptor ( u PAR ) , and cathepsin B were analyzed by immunohistochemical techniques in 11 cases of parosteal osteosarcoma and in 4 cases of dedifferentiated parosteal osteosarcoma . ^^^ Tumor cells involved in bone production ( ie , those adjacent to tumor produced bone trabeculae ) exhibited equally strong expression of u PA , u PAR , and cathepsin B , regardless of their histologic grade . ^^^ Expression of u PA , u PAR , and cathepsin B was undetectable in the `` normalized ' ' cells embedded in the well developed tumor bone trabeculae . ^^^ CONCLUSION : These data indicate that u PA and its interacting molecules , such as u PAR and cathepsin B , may have some contributory effects on the metastatic potential of tumor cells in dedifferentiated parosteal osteosarcoma . . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Expression of these proteases was compared to the following clinicopathological parameters : pathological diagnosis , tumor size , exposure to asbestos , radiotherapy , neo adjuvant chemotherapy , tumor node metastasis stage , lymph node involvement , presence of metastasis . u PA , MMP 2 , MMP 11 / STR3 , and cathepsin B were significantly increased in tumor ( the tumor : normal ratio was on average increased by 5 . 4 , 2 . 2 , 83 . 5 , and 2 . 2 fold , respectively ) . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Enzyme linked immunosorbent assays ( ELISAs ) were used to measure cysteine cathepsin B ( CatB ) and cathepsin L ( CatL ) and their inhibitors , stefin A ( StA ) and stefin B ( StB ) , together with urokinase ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , in 150 cytosols of primary invasive breast carcinoma . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| Interestingly , 6 of the 13 ozonides also inhibited cathepsin B activity . 1 Phenyl 1 , 4 epoxy 1H , 4H naphtho [ 1 , 8 de ] [ 1 , 2 ] dioxepin ( ANO 2 ) potently inhibited cathepsin B ( IC ( 50 ) = 0 . 47 microM ) as well as u PA production . ^^^ |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00749 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|