Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.56463997
BACKGROUND : Plasminogen activator inhibitor type 2 ( PAI 2 ) is a member of the serine protease inhibitor ( SERPIN ) superfamily and forms stable complexes with urokinase type plasminogen activator ( uPA ) . uPA can be found on the cell surface attached to its specific receptor ( uPAR ) , allowing for controlled degradation of the extracellular matrix by the activation of plasminogen into plasmin . 0.56463997^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.96669981
Initiation of plasminogen activation on the surface of monocytes expressing the type 2 transmembrane serine protease matriptase . uPA ( urokinase type plasminogen activator ) activates plasminogen with high efficiency when bound to its cellular receptor uPAR , but only after a prolonged lag phase during which generated plasmin activates pro uPA . 0.96669981^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.59682432
The plasminogen activating activity in normal plasma was only partially blocked ( for 77 % ) with specific antibodies to tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . 0.59682432^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.52070692
On the reversible interaction of plasminogen activator inhibitor 1 with tissue type plasminogen activator and with urokinase type plasminogen activator . 0.52070692^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.68749595
On the basis of both 125I labeled plasminogen activator binding analysis and transmission electron microscopy studies of the interaction of a plasminogen activator / gold complex with cell membranes , we have found that human keratinocytes have specific receptors for human urokinase type plasminogen activator distributed on the cell surface as singlets , or as small or large clusters . 0.68749595^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.58828103
A metastatic human melanoma cell line that produces urokinase type plasminogen activator was stably transfected with cDNA encoding human plasminogen activator inhibitor 2 ( PAI 2 ) . 0.58828103^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.56120214
Activation of plasminogen bound to surface associated MIP 2alpha by two chain urokinase type plasminogen activator ( tcu PA ) was about 2 . 5 fold more efficient than in solution ( catalytic efficiency k ( cat ) K ( M ) of 0 . 1 microM ( 1 ) s ( 1 ) , as compared to 0 . 04 microM ( 1 ) s ( 1 ) . 0.56120214^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.50648088
The complex between urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 ( plasminogen activator inhibitor 1 ) has been prognostically evaluated in patients with breast cancer . 0.50648088^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.87689246
Thus , the interactions between murine plasminogen , u PA and PAI 1 are qualitatively similar to those between their human counterparts . 0.87689246^^^ Murine plasminogen , purified from plasma as a single chain protein of 95 kDa , was resistant to quantitative activation with murine recombinant two chain u PA : only 15 % activation within 1 h at 37 degrees C was obtained in mixtures of 1 microM plasminogen and 5 nM recombinant two chain u PA , whereas quantitative activation was observed in the autologous human system . 0.59172999^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Survey of the literature and recent developments concerning the urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) may be responsible for the invasive and metastasizing capacity of tumor cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Hepatocyte directed production of urokinase type plasminogen activator ( uPA ) in transgenic mice is hepatotoxic . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MDCK and CaCo 2 cells , instead , synthesized urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It is not known if urokinase type plasminogen activator ( uPA ) is associated with normal colonic epithelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The human myeloid cell line HL 60 secretes urokinase type plasminogen activator ( uPA ) and expresses its receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
No urokinase type Plasminogen Activator ( uPA ) activity was detected in control or treated cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These cells secrete both mammalian plasminogen activators ( PAs ) , urokinase ( uPA ) and tissue type PA ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the increase of plasminogen activators ( tPA and uPA ) in the plasma during pregnancy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ovarian follicles produce two types of plasminogen activator ( tPA and uPA ) , and their inhibitor ( PAI ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have analyzed four full length cDNA clones to porcine urokinase like plasminogen activator ( uPA ) mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have isolated cDNA and genomic clones coding for porcine plasminogen activator ( urokinase , uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) mRNA is induced in macrophages by the lineage specific growth factor CSF 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , in mice , gonadotropins induce the related but distinct protease urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) is secreted as a single chain inactive zymogen ( pro uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Disregulation of urokinase plasminogen activator ( uPA ) was assessed in 134 breast cancer specimens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We previously reported that the urokinase type plasminogen activator ( uPA ) activates pro HGF in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Three of these cell lines co expressed the plasminogen activator inhibitor PAI 1 in addition to uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease involved in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In LLC PK 1 cells urokinase type plasminogen activator ( uPA ) mRNA has a short half life . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Finally , a positive correlation was found between TPK and urokinase plasminogen activator ( UPA ) ( P < 0 . 05 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) confers invasive potential to transformed cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purified inhibitor reacts with urokinase type plasminogen activator ( uPA ) and APC . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recombinant soluble urokinase receptor as a scavenger for urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation of hepatocyte growth factor by the plasminogen activators uPA and tPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The presence of urokinase type plasminogen activator ( uPA ) in human eccrine sweat has not been reported previously . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine proteinase , urokinase type plasminogen activator ( uPA ) , is also produced at that time . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor ( uPA R ) is one example of a potential aggressive marker for those cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Similarly , there was no association between CSF PAI 1 concentrations and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In LLC PK 1 cells , urokinase type plasminogen activator ( uPA ) mRNA has a short half life of 70 min . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) is involved in extracellular matrix degradation during cancer invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor invasion and metastasis is mediated in part by proteolytic enzymes including urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we analyzed the role of urokinase type plasminogen activator ( uPA ) in local immunotherapy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase type plasminogen activator ( uPA ) is a serine protease involved in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interstitial collagenase ( MMP 1 ) and urokinase plasminogen activator ( uPA ) were significantly upregulated only by bFGF . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and its receptor are essential mediators of these processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The uPA mediated pathway of plasminogen activation is central to cancer metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , mRNA levels for urokinase type plasminogen activator ( uPA ) have been found to be decreased . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a key serine protease involved in invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease causally involved in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
All lines secrete and synthesize both urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) has been associated with invasion and metastasis in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) binds to cells via a specific glycosylphosphatidylinositol anchored receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) plays a central role in tissue remodeling and cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) has been well documented in the development of pemphigus acantholysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin , generated by the endogenous plasminogen activator ( uPA ) , is not an efficient activator of proMMP 9 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two such proteases are tissue type ( tPA ) and urokinase type ( uPA ) plasminogen activators . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) play an important role in tumour invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zf 9 transcriptionally activates urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is important for matrix degradation and motility of cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) binds to its receptor ( uPAR ) with a K ( d ) of about 1 nm . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) activity is related to the malignant biological behaviour of tumours . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) and urokinase ( uPA ) levels were unchanged . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Leukocytes express both urokinase type plasminogen activator ( uPA ) and the urokinase receptor ( uPAR , CD 87 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Janus kinases Jak 1 and Tyk 2 play an important role in urokinase type plasminogen activator ( uPA ) dependent signaling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They express metalloproteinases ( MMP ) and show high levels of plasminogen activator type urokinase ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) may influence brain pathophysiology after injury . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When urokinase plasminogen activator ( uPA ) was a candidate variable in multivariate analysis , TK remained significant . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteases like urokinase type plasminogen activator ( uPA ) play an important role in tumor invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of urokinase plasminogen activator ( uPA ) in osteosarcoma is poorly understood . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although tPA shares the same function of activating plasminogen and it is structurally similar to uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is increased in human abdominal aortic aneurysm ( AAA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
ELISA demonstrated an increased fibrinolytic activity due to increased urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of the urokinase type plasminogen activator ( uPA ) to its receptor activates diverse cell signaling pathways . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of MMP 1 or urokinase type plasminogen activator ( uPA ) was not detected by Northern blot . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of bone cell markers and urokinase plasminogen activator ( uPA ) activity was also determined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This inhibitory effect was reversed with addition of exogenous urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Overexpression of urokinase plasminogen activator ( uPA ) in endothelial cells can decrease intravascular thrombosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
FGF 2 is one of the potent inducers of urokinase type plasminogen activator ( uPA ) production in endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we studied the role of urokinase plasminogen activator ( uPA ) in this process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is an important regulator of fibrinolysis in synovial fluid . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Aortic urokinase plasminogen activator ( uPA ) activity was measured by zymography after 14 days of ELS . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) converts plasminogen to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The monocytes signal the VSMCs through the urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thus the action of TAFIa could be partially overcome by plasminogen , whether lysis was by tPA , uPA or scuPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) system is involved in tumor growth and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One of the crucial molecules involved in cancer metastasis is urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) has been implicated in tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activated lymphocytes express urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The release of urokinase plasminogen activator ( uPA ) , an activator of MMPs , was also investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Selective urokinase type plasminogen activator ( uPA ) inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Leukocytes express both urokinase type plasminogen activator ( uPA ) and the urokinase receptor ( uPAR , CD 87 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , we have identified uPA ( urokinase type plasminogen activator ) as an anosmin 1 interactant . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytically active plasmin is generated from inactive plasminogen by one of 2 activators , uPA or tPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) system plays important roles in tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) binds a specific high affinity surface receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Beta2GPI is proteolytically cleaved in DV by plasmin but not by thrombin , VIIa , tissue plasminogen activator , or uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
High level expression of urokinase plasminogen activator ( uPA ) has been well documented in a variety of tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Then active uPA can convert plasminogen into plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) plays an important role in cellular invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) inhibited this PAI 1 action in a dose dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human urokinase type plasminogen activator ( uPA ) is poorly secreted by yeast cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) is implicated in both cancer cell invasion and angiogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) system has been involved in cellular invasion , angiogenesis and tumor growth . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A wide variety of tumor cells exhibit overexpression of urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we used siRNA to simultaneously target urokinase plasminogen activator ( uPA ) and its receptor , uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of pinocytosis in BMMs appears to be independent of that of urokinase type plasminogen activator expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Lung capillary endothelial cells produce and secrete urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zymographic analysis showed urokinase type plasminogen activator activity in 13 of 14 patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Bovine embryos produce a urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( u PA ) antigen levels were highest during bacterial pneumonia . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Growth factor like effect of urokinase type plasminogen activator in human renal cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor is a member of the Ly 6 superfamily . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The levels of PAI 1 were found to be correlated with those of urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor necrosis factor induction of urokinase type plasminogen activator in human endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These invasive cells synthesize both metalloproteinases and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The ligand binding domain of the cell surface receptor for urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of human urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator and its inhibitor PAI 1 in breast cancer : studies at both protein and mRNA level . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By contrast , tumor cells stained diffusely for urokinase type plasminogen activator and focally for thrombomodulin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Porcine urine , unlike human urine , does not contain detectable amounts of urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
No urokinase type plasminogen activator is detected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Nucleotide and deduced amino acid sequences of baboon urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator by rat pulmonary alveolar epithelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrin specific thrombolysis by two chain urokinase type plasminogen activator cleaved after arginine 156 by thrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Structure and characteristics of singer chain urokinase type plasminogen activator ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interaction of single chain urokinase type plasminogen activator with human endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of human endothelial cell urokinase type plasminogen activator receptor protein and messenger RNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator in rabbit tears . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increase of urokinase type plasminogen activator gene expression in human lung and breast carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Renal endothelial cells produce large amounts of single chain urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator in endothelial cells during acute inflammation of the appendix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator antigen and early relapse in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator and its inhibitor secreted by cultured human monocyte macrophages . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor cells stained for tissue factor , factor 5 , and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator : proenzyme , receptor , and inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Isolation and characteristics of urokinase type plasminogen activator from a culture of human embryo lung fibroblasts ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human synovial fibroblasts produce urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biochemical and biological properties of single chain urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator : mechanism of action and thrombolytic properties . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Picogram amounts of human urokinase type plasminogen activator ( about 0 . 002 International Unit ) are still detectable . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There was no urokinase type plasminogen activator detectable in granulomas at any time . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator in ascites obtained from the patient with mammary cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Distribution of urokinase type plasminogen activator immunoreactivity in the mouse . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
No urokinase type plasminogen activator immunoreactivity was detected in endothelial cells in the intact organism . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine proteinase urokinase type plasminogen activator was not demonstrated to be the target of PAI 2 action . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We assayed both tissue type and urokinase type plasminogen activator activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Correlation between the expression of urokinase type plasminogen activator , E cadherin and malignancy in gastric cancer ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
All of three ovarian adenocarcinoma cell lines secreted urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Up regulation of urokinase type plasminogen activator expression by the HER2 / neu proto oncogene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Changes in urokinase type plasminogen activator in orthotopic liver transplantation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Concomitant with gelatinase activity , KGF also increased urokinase type plasminogen activator in the epithelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Modulation of lipoxidase activity by urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The U 251 cell line produced a urokinase type plasminogen activator and a 92 KDa type 4 collagenase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Molecular cloning of cDNA for the bovine urokinase type plasminogen activator receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NY cells predominantly secrete single chain urokinase type plasminogen activator ( scu PA ) and some two chain u PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Membrane associated procollagenase of leukemic cells is activated by urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cellular receptor for urokinase type plasminogen activator : protein structure . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cellular receptor for urokinase type plasminogen activator : function in cell surface proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Differential biological significance of tissue type and urokinase type plasminogen activator in human breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Role of urokinase type plasminogen activator ( u PA ) in corneal epithelial migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator and plasminogen activator inhibitor in colon disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Systematic mutational analysis of the receptor binding region of the human urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator expression in human prostate carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A similar relationship was observed between staining for HAM 56 and urokinase type plasminogen activator ( p = . 02 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
No urokinase type plasminogen activator was detected in the cultures but its receptor was found in cell extracts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , urokinase type plasminogen activator transcripts were not modulated in response to PDGF BB . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zymography showed an increase in 92 kDa type 4 collagenase and urokinase type plasminogen activator at 24 hours . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor is a novel prognostic factor in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator was undetectable . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator in human preimplantation embryos . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator stimulates wound healing in the diabetic mouse . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator and its receptor in bladder cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumour cells may express urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Analysis of the forces which stabilize the active conformation of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( CD 87 ) is a pleiotropic molecule . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator ( u PA ) strongly correlates with a malignant tumor cell phenotype . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of urokinase type plasminogen activator in the pathogenesis of pemphigus ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) and plasmin activities are required for this angiogenic process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The diuretic amiloride has been reported to inhibit both Na+ H+ antiport and the urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of tissue type and urokinase type plasminogen activator activities in chronic venous leg ulcers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator system and inflammatory joint diseases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased values were caused by MMP 9 and urokinase type plasminogen activator , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interaction of urokinase type plasminogen activator receptor with integrins during cell adhesion and migration ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) is one major activator of plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
AIIt did not affect the activity of other serine proteases such as t PA or urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Natural and synthetic inhibitors of the tumor associated serine protease urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Similarly , placenta growth factor did not induce urokinase type plasminogen activator production in these cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator system is a proteolytic system involved in tissue remodeling and cell migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To evaluate the role of plasminogen activators ( PAs ) in physiological angiogenesis , we have investigated the in vivo patterns of expression of urokinase type PA ( uPA ) and PA inhibitor type 1 ( PAI 1 ) during neovascularization of ovarian follicles , the corpus luteum , and the maternal decidua . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BPAEC challenged with fragment D increased the concentration and activity of urokinase type plasminogen activator ( uPA ) in the conditioned medium within 2 to 4 h of incubation . ^^^ Fragment D also induced the release of tissue type plasminogen activator , but to a lesser extent than uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteases from the MKN 45 medium were more active than were those of MKN 28 in urokinase type plasminogen activator ( uPA ) and metal dependent serine proteinase like proteases of 75 and 68 kDa in molecular weight ( MW ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Bispecific monoclonal antibodies increase the fibrin specific fibrinolytic activity of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a mosaic glycoprotein composed of an epidermal growth factor like ( EGF ) , a kringle and a serine protease ( SP ) module . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mononuclear phagocytes regulate net PA activity by the balanced expression of urokinase type PA ( uPA ) , in either secreted or membrane associated forms , and a specific plasminogen activator inhibitor , PAI 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although prourokinase was secreted in amounts sufficient to endogenously saturate trophoblast uPA receptors , trophoblasts secreted greater amounts of PAI 1 and PAI 2 than uPA , and no net plasminogen activator activity was detected in trophoblast conditioned medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We performed a computer search of the National Biomedical Research Foundation database and found that Mo 3 is identical to the human receptor for the urokinase plasminogen activator ( uPA R ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate the role of tumor necrosis factor alpha ( TNF alpha ) in advanced collagenolysis and degradation of connective tissue components in preterm parturition , the effects of human recombinant TNF alpha ( hrTNF alpha ) on the production of matrix metalloproteinase 1 ( MMP 1 ) / tissue collagenase , MMP 3 / stromelysin , tissue inhibitor of metalloproteinases ( TIMP ) , urokinase type plasminogen activator ( uPa ) and prostaglandin ( PG ) E 2 in human chorionic cells were examined in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
On the other hand , M 27 cells produced significantly higher levels of urokinase type plasminogen activator ( uPA ) as indicated by a fibrinolysis assay and by Western blot analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of urokinase type plasminogen activator ( uPA ) to its specific cell surface receptor ( uPAR ) localises the proteolytic cascade initiated by uPA to the pericellular environment . ^^^ To this end , a bifunctional hybrid molecule consisting of the uPAR binding growth factor domain of uPA ( amino acids 1 47 ; GFuPA ) at the N terminus of plasminogen activator inhibitor type 2 ( PAI 2 ) was produced in Saccharomyces cerevisiae . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
High molecular weight urokinase type plasminogen activator ( uPA ) in which proteolytic activity was inactivated ( diisopropyl fluorophosphate ( DFP ) uPA ) , its amino terminal fragment ( ATF , amino acids ( aa ) 1 143 ) , and fucosylated and defucosylated growth factor domains ( GFD , aa 4 43 ) were tested for growth promoting effects and binding in human SaOS 2 osteosarcoma cells and U 937 lymphoma cells . ^^^ In addition , tissue plasminogen activator ( tPA ) , which has been shown to be homologous to uPA in its growth factor domain and is also fucosylated , did not inhibit 125I ATF binding nor elicit any mitogenic response . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Five rat thyroid cell lines were tested for the expression of the cell surface receptor for urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to determine the mechanism by which parathyroid hormone ( PTH ) stimulates plasminogen activator ( PA ) activity in rat osteoblasts , we investigated the effect of human PTH ( 1 34 ) [ hPTH ( 1 34 ) ] on the synthesis of mRNAs for tissue type PA ( tPA ) , urokinase type PA ( uPA ) , and PA inhibitor 1 ( PAI 1 ) , and on release of PA activity and PAI 1 protein in both normal rat calvarial osteoblasts and UMR 106 01 osteogenic sarcoma cells . hPTH ( 1 34 ) ( 0 . 25 25 nM ) decreased PAI 1 mRNA and protein , and increased PA activity in both cell types in a dose dependent manner with ED 50 of about 1 nM for both responses . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We show in the present study that urokinase plasminogen activator ( uPA ) localization in the human keratinocyte is similarly dependent upon calcium concentration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cellular urokinase type plasminogen activator ( uPA ) receptor ( uPAR ) is a glycolipid anchored membrane protein thought to be involved in pericellular proteolysis during cell migration and tumor invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The human sarcoma cell line HT 1080 was found , by in situ hybridization , to consist of cells expressing various levels of urokinase ( uPA ) and tissue type ( tPA ) plasminogen activator ( PA ) suggesting clonal variation of expression of these genes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At 2 week intervals the cells were tested on the CAM for metastatic ability and assayed for expression of urokinase type plasminogen activator ( uPA ) and the M ( r ) 92 , 000 and M ( r ) 72 , 000 gelatinase / type 4 collagenases , enzymes the expression of which has previously been shown to correlate with tumor cell dissemination . ^^^ Expression of proteins which modulate the degradative potential of these enzymes , plasminogen activator inhibitors 1 and 2 ( PAI 1 , PAI 2 ) , uPA receptor , and tissue inhibitors of metalloproteases 1 and 2 ( TIMP 1 and TIMP 2 ) , were also assayed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have characterized a transcriptional enhancer of the human urokinase type plasminogen activator ( uPA ) gene and found a regulatory element required for co operation between a PEA 3 AP 1 element and an AP 1 site in the enhancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This morphological change is accompanied by the accumulation of the usually visceral endoderm specific marker urokinase type plasminogen activator ( uPA ) and an increase in tPA levels in comparison to untreated RACF 9 controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The regulation of urokinase plasminogen activator ( uPA ) gene expression by the two major cAMP dependent protein kinase isozymes was studied in SC 115 mouse mammary carcinoma cells using the site selective cAMP analog approach . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In confirmation of our earlier studies , all the invasive cell lines expressed high levels of surface bound urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a proteolytic enzyme able to convert the zymogen plasminogen into the strong protease plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of plasminogen activator and plasminogen activator inhibitor mRNA in human fibroblasts grown on different substrates . mRNA levels for urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) were examined in human diploid ( neonatal foreskin ) fibroblasts grown in 200 ml microcarrier suspension culture . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
With this method , we have obtained findings suggesting that plasminogen and plasminogen activators ( PAs ) , especially urokinase type PA ( uPA ) derived from endothelial cells , enhance angiogenic activity , probably by increasing endothelial migration . uPA was immunohistochemically observed to be primarily cell associated on the focal contract areas , probably via its receptors on endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The two molecular forms ( 47 kDa and 28 kDa ) of plasminogen dependent protease were demonstrated by casein zymography , and it was suggested that they were urokinase type plasminogen activators ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Complexes between 125I labeled urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) bound to purified alpha 2 macroglobulin ( alpha 2M ) receptor ( alpha 2MR ) / low density lipoprotein receptor related protein ( LRP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recent observations have indicated that TNF can promote the expression , synthesis and secretion of urokinase plasminogen activator ( uPA ) in low passage human umbilical vein endothelial cells which normally synthesize little uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The development of a simple , sensitive fluorimetric assay for the measurement of cell surface associated urokinase plasminogen activator ( uPA ) on viable , adherent HCT 116 cells in microtitre plates , after a preincubation with purified human plasminogen is described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators were identified by fibrinolytic autography in the sulcus epithelium of human gingival mucosa but not in the orthokeratinized gingival epithelium . ^^^ Plasminogen activators could not be demonstrated in either the sulcus or gingival epithelium by immunofluorescence , but both uPA and tPA were found in occasional squamous carcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In cells transformed by activated mammalian EJ ras and chimeric EJ / vHa ras , high constitutive levels of urokinase plasminogen activator ( uPA ) mRNA and / or phorbol 12 myristate 13 acetate ( PMA ) inducibility of the uPA mRNA was observed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The characteristics of the plasminogen activators , the uPA receptor and the plasminogen activator inhibitors as well as their expression and regulation in tumors and tumor cell lines are reviewed . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Basolateral accumulation of urokinase type plasminogen activator ( uPA ) , but not its apical targeting , required an acidic compartment and the integrity of the cytoskeleton . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Normal osteoblast like cells had low to undetectable basal urokinase ( uPA ) and tissue plasminogen activator ( tPA ) activity , which was significantly stimulated by TGF beta 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Treatment of CM with a polyclonal antibody against human urokinase type plasminogen activator ( uPA ) not only inhibited uPA activity , but also significantly reduced PMNL chemoattractant activity when compared with untreated CM . ^^^ Furthermore , when CM was passed over an anti uPA immunoaffinity column , plasminogen activator activity was reduced 90 % and chemoattractant activities was reduced 68 % . ^^^ Both plasminogen activator and chemoattractant activities were reconstituted in the eluate from the anti uPA column . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase type plasminogen activator is important for matrix degradation and motility of cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) contributes to tumor progression in prostate cancer ( CaP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Receptor bound urokinase type plasminogen activator ( uPA ) appears to play a key role in these events . uPA converts plasminogen into plasmin and thus mediates pericellular proteolysis during cell migration and tissue remodeling under physiological and pathophysiological conditions . uPA is secreted as an enzymatically inactive proenzyme ( pro uPA ) by tumor cells and stroma cells . uPA exerts its proteolytic function on normal cells and tumor cells as an ectoenzyme after having bound to a high affinity cell surface receptor . ^^^ Receptor bound enzymatically active uPA converts plasminogen to plasmin which is bound to a different low affinity receptor on tumor cells . ^^^ Hence receptor bound uPA will promote plasminogen activation and thus the dissolution of the tumor matrix and the basement membrane which is a prerequisite for invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Clinical significance of urokinase type plasminogen activator ( uPA ) in invasive cervical cancer of the uterus . ^^^ The relationship between the level of urokinase type plasminogen activator ( uPA ) and pelvic lymph node metastasis was investigated in 20 patients with invasive cervical cancer of the uterus . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recently , we have shown that plasminogen activators ( PAs ) of both types , urokinase type ( uPA ) as well as tissue type ( tPA ) , are involved in the in vitro invasiveness of human melanoma cells . ^^^ The activation of the proenzyme plasminogen bound on MelJuso cells , which expressed surface associated uPA activity , occurred almost synchronously with binding to the cell surface . ^^^ In conclusion , these data provide evidence that plasminogen activation associated with the surface of human melanoma cells is catalyzed much more efficiently by cell associated uPA ( MelJuso ) than by secreted tPA ( MeWo ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enzymatic properties of phosphorylated urokinase plasminogen activator ( P uPA ) ( 1 ) extracted from human carcinomatous cell line Detroit 562 cells were compared with those of non phosphorylated uPA of urinary origin ( nP uPA ) . ^^^ Using plasminogen as a substrate , the Km and Kcat of P uPA were higher than that of nP uPA while the Kcat / Km was lower . ^^^ P uPA had a low affinity for the inhibitors of plasminogen activator PAI 1 and PAI 2 , and was inhibited only by the excess amounts of inhibitors . ^^^ These alterations by phosphorylation enable uPA to be more efficient in a focal proteolysis through plasminogen activation . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effective activation of the proenzyme form of the urokinase type plasminogen activator ( pro uPA ) by the cysteine protease cathepsin L . ^^^ Increased levels of both the cysteine protease , cathepsin L , and the serine protease , uPA ( urokinase type plasminogen activator ) , are present in solid tumors and are correlated with malignancy . uPA is released by tumor cells as an inactive single chain proenzyme ( pro uPA ) which has to be activated by proteolytic cleavage . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interferon alpha ( Intron A ) upregulates urokinase type plasminogen activator receptor gene expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect of therapeutic and pharmacological concentrations of tiaprofenic acid , a non steroidal anti inflammatory drug ( NSAID ) , on the synthesis of the plasminogen activators , urokinase plasminogen activator ( uPA ) and tissue plasminogen activator ( tPA ) , and the plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) , by human synovial membranes isolated from osteoarthritis ( OA ) and rheumatoid arthritis ( RA ) sufferers was evaluated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The regulation of urokinase plasminogen activator ( uPA ) expression was investigated in 2 highly metastatic rat mammary adenocarcinoma cell lines , BC 1 and MAT 13762 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a specific inhibitor of the serine proteases tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have recently shown that alveolar epithelial cells may control fibrinolysis by expressing both urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 . ^^^ In this study , monolayers of rat alveolar epithelial cells were used as a model of the alveolar surface and were found to lyse plasma derived fibrin matrices by a process that was plasminogen and uPA dependent . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease which has frequently been implicated in the process of tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Production of Sertoli cell tissue type plasminogen activator ( tPa ) was stimulated by FSH at each of the developmental stages examined , whereas production of urokinase type plasminogen activator ( uPa ) was influenced by FSH only in prepubertal Sertoli cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HOC 1 ovarian cancer cells express the single chain form of the urokinase type plasminogen activator ( uPA ) and cathepsin B ( cath B ) on their cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In 30 patients , aqueous humor and plasma were compared for their content of urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen activators inhibitors ( PAIs ) , plasminogen , and total proteins . ^^^ In addition to uPA , aqueous humor contained lower levels of tPA , but no detectable levels of reactive plasminogen activators inhibitors ( PAIs ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
F 9 cells treated with NaB synthesize both tissue type ( tPA ) and urokinase type ( uPA ) plasminogen activator , though RA induces only tPA production . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interactive regulation of gene expression by retinoic acid ( RA ) and adenosine monophosphate ( cAMP ) in mammary tumor cells was explored using Shionogi mouse mammary carcinoma cells ( SC 115 ) as a model and urokinase type plasminogen activator ( uPA ) as a target gene product . ^^^ Experiments utilizing the casein / plasminogen overlay method for the detection of uPA secretion by increased rate of uPA secretion per cell rather than to an increased fraction of uPA secreting cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Exposure of bovine capillary endothelial cells to transforming growth factor beta 1 , which inhibits uPA production and upregulates type 1 plasminogen activator inhibitor , the major endothelial cell plasminogen activator inhibitor , has no effect on uPA receptor levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To establish a possible association between the presence of collagenase ( C ) , urokinase type plasminogen activator ( uPA ) and the neoplastic growth of the endometrium , 44 endometrial specimens ( 14 proliferative , 11 secretive , 7 adenomatous hyperplasia , 12 adenocarcinoma ) were studied using immunohistochemistry with antisera for C , uPA , EGF receptors and TGF alpha . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) activity in sera of patients suffering from nasopharyngeal carcinoma was examined by a uPA specific immunocapture assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Highly charged polyanionic ligands of the scavenger receptor trigger macrophage secretion of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Numerous studies have linked the production of increased levels of urokinase type plasminogen activator ( uPA ) with the malignant phenotype . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of 125I labelled urokinase plasminogen activator ( uPA ) to cell membranes of ovarian tumours was characterised . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In situ hybridization showed differential expression of the two mRNAs in mouse gastric mucosa . muPAR 1 mRNA is located in luminal epithelial cells situated close to urokinase type plasminogen activator producing connective tissue cells of the lamina propria , pointing to plasmin generation controlled by the cooperation of different cells that may play a role in the release of gastric epithelial cells . muPAR 2 mRNA is expressed in the basal epithelial cells , and the deduced protein sequence includes the receptor ligand binding domain , but omits the region involved in glycolipid mediated membrane anchoring , suggesting that muPAR 2 may code for a secreted uPA binding protein . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Caseinolytic plaque assay , immunofluorescence analysis , direct binding studies and cross linking experiments show that the transfected mouse LB 6 cells specifically bind human uPA , which in turn activates plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is expressed at higher levels in many transformed cells as compared with their non transformed counterparts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Spontaneous intestinal and intra abdominal bleeding was observed in a high percentage of newborn transgenic mice carrying the murine urokinase type plasminogen activator ( uPA ) gene linked to the albumin enhancer / promoter . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The inhibitors were expressed in Escherichia coli , and the purified proteins had specific activities toward urokinase type plasminogen activator ( uPA ) or the single and two chain forms of tissue type plasminogen activator ( tPA ) that were similar to wtPAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the porcine renal epithelial cell line , LLC PK 1 , activation of the cAMP dependent signal transduction pathway induces the urokinase type plasminogen activator ( uPA ) gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human colon adenocarcinomas and adjacent normal colon tissues were stained immunohistochemically with three different monoclonal antibodies and one preparation of polyclonal antibodies against each of the two plasminogen activators , uPA ( urokinase type ) and tPA ( tissue type ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A transcriptional silencer has been identified in the 5 ' regulatory region of the human urokinase plasminogen activator ( uPA ) gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of the urokinase type plasminogen activator ( uPA ) gene in LLC PK 1 cells can be induced by signals mediated by both cAMP dependent protein kinase ( PKA ) and Ca ( 2+ ) and phospholipid dependent protein kinase ( PKC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vitro , PCI inhibits activated protein C ( APC ) , thrombin , plasma kallikrein ( KK ) and urokinase ( uPA ) and tissue type plasminogen activator ( tPA ) , and we have shown in vivo inhibition of APC , uPA and KK by PCI . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recent observations have indicated that TNF can promote the synthesis and secretion of urokinase plasminogen activator ( uPA ) in low passage human endothelial cells that normally release little uPA . ^^^ Analysis of cell free conditioned medium derived from gamma IFN treated cultures by micro enzyme linked immunosorbent assay ( ELISA ) methodologies using uPA and plasminogen activator inhibitor type 1 ( PAI 1 ) specific monoclonal antibodies ( MoAbs ) indicate that the decrease in uPA activity observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS PAGE ) zymography is a direct result of a decrease in extracellular uPA antigen and is not a consequence of increased PAI 1 antigen . ^^^ The observed increase in ECM degradation mediated by TNF treatment of endothelial cells was dependent on the presence of plasminogen and could be inhibited by an anticatalytic uPA MoAb implying the requirement of proteolytically active uPA in this process . gamma IFN ( 25 ng / mL ) treatment of endothelial cells antagonized TNF promoted degradation of radiolabeled ECM at a concentration that completely inhibited TNF mediated uPA expression and activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The dependence of urokinase type plasminogen activator ( uPA ) induction on endogenous basic fibroblast growth factor ( bFGF ) activity during endothelial cell migration was investigated utilizing a combination of wounded endothelial cell monolayers and substrate overlay techniques . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The 5 ' flanking regions of the mouse and pig urokinase plasminogen activator ( uPA ) genes were sequenced and sequence homology interrupted by repeat elements was found to extend to 4 . 6kb in pig and 6 . 6kb in mouse . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease implicated in cancer spread and is thus a potential prognostic marker for human cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Finding glucocorticoids ( GC ) to also inhibit IFN gamma induced HLA DR expression and to regulate serine protease , especially urokinase plasminogen activator ( uPA ) , activity raised the possibility of GC regulation of TLP activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biological and clinical relevance of the urokinase type plasminogen activator ( uPA ) in breast cancer . ^^^ An enzymatically inactive proenzyme form of the urokinase type plasminogen activator ( pro uPA ) is secreted by tumor cells which may be converted to an enzymatically active two chain uPA molecule ( HMW uPA ) by plasmin like enzymes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By immuno capture and immuno inhibition studies we obtained evidence that HaCaT cells synthesize and secrete urokinase type plasminogen activator ( uPA ) and tissue type PA ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Analysis of enzymatic activity indicates that under conditions where syn uPA and std uPA are completely inhibited by endothelial type plasminogen activator inhibitor ( PAI 1 ) , mut uPA retains 90 % activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The specific cellular receptor for urokinase type plasminogen activator ( uPA ) is found on a variety of cell types and has been postulated to play a central role in the mediation of pericellular proteolytic activity . ^^^ We have studied the kinetics of plasminogen ( Plg ) activation catalyzed by uPA specifically bound to its receptor on the human monocytoid cell line U 937 and demonstrate this process to have properties differing widely from those observed for uPA in solution . ^^^ In contrast to intact cells , purified uPA receptor ( isolated from phorbol 12 myristate 13 acetate stimulated U 937 cells ) was observed to partially inhibit uPA catalyzed Plg activation , although activity against low molecular weight substrates was retained . ^^^ Therefore , the cellular binding of Plg appears to be of critical importance for the efficient activation of Plg by receptor bound uPA . ^^^ Together , these data demonstrate that the cell surface constitutes the preferential site for Plg activation when uPA is bound to its specific cellular receptor , which therefore has the necessary characteristics to play an efficient role in the generation of pericellular proteolytic activity . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human tumor cell urokinase type plasminogen activator ( uPA ) : degradation of the proenzyme form ( pro uPA ) by granulocyte elastase prevents subsequent activation by plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) gene expression in LLC PK 1 cells is induced by activation of cAMP dependent protein kinase ( cAMP PK ) or protein kinase C ( PK C ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human glomerular epithelial cells ( GECs ) in culture synthesize single chain , urokinase type plasminogen activator ( SC uPA ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) and possess specific membrane binding sites for u PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator ( scu PA ) purification by immuno affinity chromatography ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These enzymes are active toward the substrate , plasminogen , as well as toward the uPA specific synthetic substrate , Spectrozyme UK , and these activities are inhibitable by diisopropylphosphorofluoridate ( DFP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Renal epithelial cell mutants were compared to the LLC PK 1 parental cell line for induction of the cAMP responsive urokinase type plasminogen activator ( uPA ) gene , as quantitated by the technique of mRNA solution hybridization . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study evaluates the contribution of two types of plasminogen activators ( PAs ; tissue type PA ( tPA ) versus urokinase type PA ( uPA ) toward the invasiveness of human melanoma cells in a novel in vitro assay . ^^^ MelJuso cells produced uPA as well as plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Our results confirm the importance of uPA catalyzed plasminogen activation in tumor cell invasiveness . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Calcitriol induced differentiation of U 937 mononuclear phagocytes is known to have divergent effects on the synthesis of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 2 ( PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PA inhibitor type 1 ( PAI 1 ) is an efficient inhibitor of tissue type PA ( tPA ) and urokinase type PA ( uPA ) that may therefore be instrumental for the control of plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cathepsin B efficiently activates the soluble and the tumor cell receptor bound form of the proenzyme urokinase type plasminogen activator ( Pro uPA ) . ^^^ Action of purified human cathepsin B on recombinant single chain urokinase type plasminogen activator ( pro uPA ) generated enzymatically active two chain uPA ( HMW uPA ) , which was indistinguishable by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot from plasmin generated HMW uPA and from elastase or thrombin generated inactive two chain urokinase type plasminogen activator . ^^^ The cleavage site within the cathepsin B treated urokinase type plasminogen activator ( uPA ) molecule , determined by N terminal amino acid sequence analysis , is located between Lys 158 and Ile 159 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The recombinant PAI 2 formed SDS stable complexes with urokinase and tissue type plasminogen activator and inhibited the proteases with similar reaction kinetics to placental PAI 2 ( second order rate constant for uPA , 2 . 4 10 10 ( 6 ) M 1 s 1 , and for two chain tPA , 0 . 7 10 10 ( 5 ) M 1 s 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Rat alveolar epithelial cells produce large quantities of urokinase type plasminogen activator ( uPA ) in vitro , and uPA expression is modulated in association with cellular differentiation and exposure to inflammatory mediators . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transcription of the human urokinase type plasminogen activator ( uPA ) gene in HeLa cells is induced by phorbol myristate acetate ( PMA ) , interleukin 1 ( IL 1 ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator showed no major expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Phorbol esters , by activating protein kinase C ( PKC ) , induce the expression of the urokinase type plasminogen activator ( uPA ) gene and the proto oncogene c fos in LLC PK 1 ( PK 1 ) porcine kidney epithelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
For the same seminal samples , tissue plasminogen activator ( t PA ) , urinary plasminogen activator ( uPA ) antigens and uPA activity were specifically quantified . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To assess the postulated role of plasminogen activation in tumor invasion , we have investigated the cellular sites of synthesis for urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators and their inhibitors ( PAI 1 and PAI 2 ) in two human cutaneous neoplasia that differ in their metastatic potential . ^^^ These observations establish that invasive human malignant cells in vivo can activate plasminogen through uPA production during the early phases of tumor growth ; they also demonstrate that the proteolytic activity of tumor cells can be modulated by the concomitant production of PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have characterized a transcriptional enhancer of the human urokinase type plasminogen activator ( uPA ) gene in three transformed human cell lines : HeLa , HepG 2 and HT 1080 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of cathepsin D ( Cath D ) , urokinase ( uPA ) and two plasminogen activator inhibitors ( PAI 1 and PAI 2 ) were analysed in the cytosols of 130 human mammary tumours ( 43 benign tumours and 87 primary and unilateral breast carcinomas ) . uPA , PAI 1 and PAI 2 levels were measured by antigenic immunoassays and Cath D by immunoradiometric assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We examined the production of tissue type ( tPA ) and urokinase type plasminogen activator ( uPA ) activity by three types of osteoblast like cells ( normal rat osteoblasts , rat and human osteosarcoma cells ) using a quantitative spectrophotometric assay and a qualitative gel overlay technique . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The extracellular localizations of urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were examined in cultured bovine capillary endothelial cells ( BCEs ) by an immunofluorescence method using BCEs treated with or without saponin and focal contact preparations . ^^^ These findings suggest that uPA and PAI 1 are located under BCEs participating in the regulation of proteolytic activities provoked by plasminogen PAs plasmin system in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To understand the relationship between protein synthesis and mRNA stability , we studied the degradation of calcitonin induced urokinase type plasminogen activator ( uPA ) mRNA in LLC PK cells . uPA mRNA became highly stable by pretreatment with either cycloheximide or pactamycin , and the stabilizing effect of cycloheximide treatment was time dependent with the full effect exerted by 60 min . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Of the examined tumors , 67 % showed a high expression of transforming growth factor alpha ( TGF alpha ) ; 50 % for epidermal growth factor ( EGF ) , 45 % for EGF receptor ( EGFR ) , and 30 % for urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Low passage cultures of normal human keratinocytes produce several components of the plasminogen activator / plasmin proteolytic cascade , including urokinase plasminogen activator ( uPA ) , tissue plasminogen activator ( tPA ) , and two specific inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Nude mice given inoculations s . c . of a human squamous carcinoma HEp 3 ( 1 . 5 10 10 ( 6 ) cells / mouse ) developed invasive tumors that produced high levels of urokinase type plasminogen activator ( uPA ) and metastasized predictably to the lungs and lymph nodes of the host . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator urokinase ( uPA ) seems to play a central role as it was shown that elevated content of uPA is correlated with a high risk of early relapse and shorter overall survival , at least in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To explore this relationship , we have measured steady state levels of mRNA coding for urokinase plasminogen activator ( uPA ) , tissue plasminogen activator ( tPA ) , transin and tissue specific inhibitor of metalloproteinases ( TIMP ) ; as well as gelatinolytic , caseinolytic and plasminogen activator activities secreted by SPI , a non metastatic mouse mammary carcinoma cell line and 4 metastatic sublines derived from it . mRNA encoding metalloproteinase transin was increased 15 to 20 fold , while TIMP transcripts were decreased 3 fold in the metastatic sublines compared to parental SPI tumor cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Limited proteolysis of cell bound fibronectin may occur with several proteinases : 1 ) uPA having a receptor in the focal contact of a cell ; 2 ) plasmin resulted from plasminogen under the action of uPA ; 3 ) stromelysin whose synthesis is induced by fibronectin proteolytic fragments ; 4 ) metalloproteinases secreted by some cells and involving in the hapatotactic motion of a cell over fibronectin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) was distributed evenly throughout the colony , while , as we have demonstrated previously , urokinase type plasminogen activator ( uPA ) was preferentially localized at the migrating edges of the colony . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) has been implicated in cellular migration accompanying different biological phenomena including organogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human and murine urokinase cDNAs linked to the murine alpha A crystallin promoter exhibit lens and non lens expression in transgenic mice . cDNAs encoding either the human or the murine urokinase type plasminogen activator ( uPA ) were fused downstream from the promoter enhancer element of the murine gene encoding alpha A crystallin , a protein found exclusively in the ocular lens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have discovered that the product of the human urokinase plasminogen activator gene , pro uPA , is phosphorylated in serine in at least two human cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemistry using biotin streptavidin peroxidase indicated the presence of both tissue type plasminogen activator ( tPA ) and urokinase ( uPA ) within the cytoplasm of osteoclasts isolated from newborn rat long bones . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Isotopically labeled [ ( 3H ] serine , [ 3H ] proline , and [ 35S ] sulfate ) subendothelial cell basement membranes were used to determine the role of urokinase plasminogen activator ( uPA ) and its specific inhibitor plasminogen activator inhibitor 2 ( PAI 2 ) in colon cancer cell extracellular matrix degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both urokinase ( uPA ) and tissue type plasminogen activator ( tPA ) activities corresponding to proteins of 38 kDa and 65 kDa molecular masses , were detected in the extracts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Supernatant obtained from granulocytes stimulated in the presence of cytochalasin B by the chemotactic peptide N formyl norleucyl leucyl phenylalanyl norleucyl tyrosyl lysine displayed an inhibitory effect on the plasmin dependent conversion of tumor urokinase type plasminogen activator proenzyme ( pro uPA ) to the active form of uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to normal epidermis , lesional psoriatic epidermis contains primarily tissue type plasminogen activator ( tPA ) activity and much lower levels of urokinase type plasminogen activator ( uPA ) activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Based on amino acid sequencing and estimated molecular weight , this peptide was identified as an NH 2 terminal fragment of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The correlation between urokinase type plasminogen activator ( uPA ) expression and tumor cell invasion and metastasis has been well documented . ^^^ Net uPA activity is determined not only by the amount of the enzyme itself , but also by its state of activation and the amount of specific plasminogen activator inhibitors ( PAIs ) present . ^^^ Both uPA and its substrate , plasminogen , can bind to cells via specific membrane associated receptors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Gene transfer techniques were utilized to evaluate the role of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) in enhancing or preventing the expression of the invasive malignant phenotype , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumour associated fibrinolysis : the prognostic relevance of plasminogen activators uPA and tPA in human breast cancer . ^^^ The urokinase type plasminogen activator ( uPA ) which is secreted by tumour cells as an enzymatically inactive proenzyme ( pro uPA ) seems to play a key role in mediating tumour cell invasion in cancer tissues . ^^^ Receptor bound uPA converts enzymatically inactive plasminogen into the serine protease plasmin which then degrades the extracellular matrix surrounding the tumour cells ( tumour stroma ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Additionally , TSP . uPA complexes were found to have plasminogen activating activity equivalent to fluid phase uPA and to be protected from inhibition by plasminogen activator inhibitor type 1 , the major plasma and matrix plasminogen activator inhibitor . ^^^ Complex formation of TSP with uPA may serve to localize , concentrate , and protect these enzymes on cell surfaces and within the extracellular matrix , thereby providing a reservoir of plasminogen activator activity . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Similarly , HT 1080 cells , which produce high levels of urokinase type plasminogen activator ( uPA ) , were poorly invasive under standard conditions but invasion was enhanced by plasmin inhibitors or anti uPA antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibitors of this proteinase system and murine monoclonal antibodies ( MAb ) specific for human urokinase plasminogen activator ( uPA ) decreased the SqCC mediated digestion of radiolabeled ECM in a concentration dependent manner . ^^^ These findings were specific for uPA , since cell surface associated tissue plasminogen activator was not detected in these cell types under analogous conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin formation , dependent on the urokinase type plasminogen activator ( uPA ) , is discussed in its biochemical , regulatory and physiological aspects and its involvement in cancer malignancy analysed . ^^^ The ability of cells to produce plasmin on their surface , due to the presence of uPA and plasminogen receptors , is at the basis of the regulation of the plasminogen activating system in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Secretion of urokinase type plasminogen activator ( uPA ) by chicken embryo fibroblasts ( CEF ) is increased approximately 50 fold following transformation by Rous sarcoma virus ( RSV ) . ^^^ Using a cloned and fully sequenced chicken uPA cDNA probe , we have established that this increase in plasminogen activator production can be largely accounted for by an increase in cellular uPA mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase plasminogen activator ( uPA ) has been previously shown not to internalize its ligand , but rather to focalize its activity at the cell surface , allowing a regulated cell surface plasmin dependent proteolysis . ^^^ On the surface , uPA can activate surface bound plasminogen to produce surface bound plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cell membranes from ten non small cell lung cancers and four specimens of adjacent lung tissue were assessed for the presence of urokinase type plasminogen activator ( uPA ) receptors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) binds to a specific receptor on various cell types , the bound molecule retaining its enzymatic activity against plasminogen . ^^^ We have now investigated whether receptor bound uPA also retains the ability to react with and be inhibited by plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . uPA bound to its receptor on human U 937 monocyte like cells was inhibited by PAI 1 ( in its active form in the presence of vitronectin fragments ) with an association rate constant of 4 . 5 10 10 ( 6 ) M 1 s 1 , which was 40 % lower than that obtained for uPA in solution ( 7 . 9 10 10 ( 6 ) M 1 s 1 ) . ^^^ These constants although lower than those for uPA in solution still represent rather rapid inhibition of the enzyme , and demonstrate that uPA bound to its specific cellular receptor remains available for efficient inhibition by PAI ' s , which may therefore play a major role in controlling cell surface plasminogen activation and extracellular proteolytic activity . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Receptor bound urokinase type plasminogen activator ( uPA ) remains associated to the surface of human monocytes for many hours . ^^^ Receptor bound enzyme can be inhibited by plasminogen activator inhibitor 2 ( PAI 2 ) , with a kinetics comparable to that determined for the free enzyme , and uPA / PAI 2 complexes can bind to the uPA receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown that the proliferation of cultured astrocytes can be stimulated by the urokinase type ( uPA ) of plasminogen activator ( PA ) and that astrocytes are able to release such uPA upon stimulation with basic fibroblast growth factor , which is known to act as a mitogen for these cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The amino terminal portion of urokinase type plasminogen activator ( uPA ) interacts with a cell surface binding protein / receptor that recognizes a region of the molecule autonomous from that of the catalytic domain of the enzyme , which mediates the conversion of plasminogen to plasmin . ^^^ Treatment of A 431 squamous carcinoma cells ( SqCC ) with epidermal growth factor ( EGF ) has previously been shown to result in an induction of the synthesis and extracellular accumulation of uPA and the plasminogen dependent proteolysis of extracellular matrix ( ECM ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The hormonal regulation of the human urokinase type plasminogen activator ( uPA ) gene has been studied by introducing into mouse and rat Sertoli cell primary cultures a recombinant plasmid , in which the transcription regulatory elements of the cloned human uPA gene drive the expression of the bacterial chloramphenicol acetyl transferase gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human alveolar macrophages are known to synthesize urokinase ( uPA ) and a specific plasminogen activator inhibitor , PAI 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This complex system includes : the proenzymes of tissue type PA ( tPA ) and urokinase type PA ( uPA ) ; the active enzymes tPA , uPA and plasmin ; the substrate plasminogen ; several natural inhibitors of PA and plasmin activity ; and the cellular receptors that bind the proenzymes , enzymes , and inhibitor enzyme complexes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human neuronal brain cultures established from 12 and 14 week old fetuses synthesize and secrete urokinase type plasminogen activator ( uPA ) and limited amounts of tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By use of both normal and SV 40 T antigen immortalized cells , it was found that treatment with TGF beta 1 transiently increases mRNA levels for urokinase ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) approximately 5 and 50 fold , respectively , within 4 h . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator activity , determined in the absence of fibrin in tear samples collected by capillary tubes at low flow rates , is considered to be the result of the presence of urokinase type plasminogen activator ( uPA ) deriving from the epithelial cells of the cornea and the conjunctiva . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urinary levels of urokinase type plasminogen activator and its receptor in the detection of bladder carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The chicken urokinase type plasminogen activator ( uPA ) cDNA and gene have been isolated and the complete nucleotide sequence of each established . cDNA sequence and Northern blot RNA analysis indicate that the chicken uPA mRNA is approximately 2500 nucleotides in size and contains a large 3 ' noncoding region ( 998 nucleotides ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mononuclear phagocytes regulate the generation of plasmin by secreting urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 2 ( PAI 2 ) . ^^^ Similar to U 937 monoblast like cells and peripheral blood monocytes ( PBM ) , THP 1 cells produce a PA that is specifically neutralized by anti uPA antibody and comigrates with human high molecular mass uPA ( 54 kDa ) on casein plasminogen zymogaphy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The presence and localization of the plasmin system components urokinase ( UPA ) , tissue type plasminogen activator ( TPA ) , plasminogen ( PG ) , a neoantigen expressed by the plasmin alpha 2 antiplasmin complex , and plasmin inhibitors alpha 2 antiplasmin ( AP ) and alpha 2 macroglobulin ( MG ) have been tested by immunofluorescence on sections of 11 benign and 40 malignant lesions of the breast in an attempt to apply a morphological approach to the problem of tumor invasion in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human monocytes / macrophages produce plasminogen activator specific inhibitors ( PAIs ) that form covalent complexes with urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Preincubation with monoclonal or polyclonal antibodies to urokinase type plasminogen activator ( uPA ) totally inhibited the IM inducible shape change and adhesion loss . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Based on investigations on common warts , we studied the effect of cryotherapy on the immunohistological detection of intermediate filaments ( cytokeratin , vimentin / desmin , tubulin ) and plasminogen activators of the urokinase type ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The amino terminal fragment ( ATF ) of urokinase type plasminogen activator ( uPA ) is a degradation product comprising the entire growth factor like and kringle domains . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , anti uPA IgG markedly inhibited the enhancing effect of plasminogen on the 4th and 7th days of culture ( p less than 0 . 01 ) , whereas anti tPA IgG showed an inhibitory tendency only on the 4th day of culture ( p less than 0 . 05 ) . ^^^ These findings indicate that the plasminogen PAs system , especially uPA synthesized and secreted by BCEs , plays an important role in regulating angiogenesis . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The observed increase in ECM degradation mediated by EGF in A 431 tumor cells was dependent upon the presence of plasminogen and could be inhibited by an anticatalytic uPA monoclonal antibody . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Secretion of urokinase type plasminogen activator ( uPA ) by RAW264 . 7 cells was stimulated by heparin in a dose and time dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recently two types of plasminogen activator tissue type PA ( tPA ) and urokinase type PA ( uPA ) have been detected in human plasma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Sympathetic neurons release both urokinase plasminogen activator ( uPA ) and tissue plasminogen activator ( tPA ) . ^^^ Inhibition of plasminogen activator ( PA ) activity but not other serine protease activity correlates with the increase in neurite outgrowth ( uPA , r = 0 . 89 ; tPA , r = 0 . 86 ; plasmin , r = 0 . 015 ; thrombin , r = 0 . 025 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) inhibits both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) and , therefore , is an important regulator of plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
During mouse embryogenesis , visceral endoderm secretes urokinase type plasminogen activator ( uPA ) whereas parietal endoderm secretes tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytic enzymes released from granulocytes upon stimulation with the chemotactic N formyl peptide FNLPNTL ( in the presence of cytochalasin B ) prevented activation of tumor cell single chain urokinase type plasminogen activator ( pro uPA ) by plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human urokinase type plasminogen activator ( uPA ) binds rapidly and with high affinity to a number of human cell types ; this localizes plasmin generation to the close environment of the cell surface . uPA binding to HeLa and U 937 cells is mediated by a single class of sites with an affinity of 3 . 4 + / 1 . 3 10 10 ( 10 ) M . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have used cRNA probes for urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators to analyze their mRNA levels in different stages of the epithelial cycle . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A specific surface receptor for urokinase plasminogen activator ( uPA ) recognizes the amino terminal growth factor like sequence of uPA , a region independent from and not required for the catalytic activity of this enzyme . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) interacts with a surface receptor and with specific inhibitors , such as plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^ Plasminogen activator activity of these cells is dependent on exogenous uPA , is competed for by receptor binding diisopropyl fluorophosphate treated uPA , and is inhibited by the addition of PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both murine and human tPA compete with 125I tPA for binding , with both murine and human urokinase ( uPA ) as well as human thrombin and plasminogen fail to compete . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This fibrinolytic activity was attributed to the presence of a plasminogen activator , which was immunochemically identified as urinary type plasminogen activator ( uPA ) by antibody neutralization assay , immunoblotting , and immunofluorescence . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In LLC PK 1 cells , as well as in the mutants M 18 and FIB 5 , the extent of the induction of urokinase type plasminogen activator ( uPA ) by the cyclic AMP mediated effectors calcitonin , vasopressin and forskolin was directly correlated with the levels of activated catalytic subunit . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have developed a homologous cell free transcription system using extracts from the porcine kidney cell line LLC PK 1 to study the molecular mechanisms by which cAMP regulates urokinase type plasminogen activator ( uPA ) gene transcription . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In LLC PK 1 cells , a cyclic AMP ( cAMP ) elevating peptide hormone , calcitonin , induces urokinase type plasminogen activator ( uPA ) gene transcription without concomitant protein synthesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The murine urokinase type plasminogen activator ( uPA ) gene has been isolated from a BALB / c liver DNA cosmid library and its nucleotide sequence established . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Scatchard analysis and dose response curves showed a parallel increase in the number of receptors on U 937 cells capable of accepting exogenous plasminogen and urokinase ( UPA ) synthetized by differentiating U 937 monocytic cells . ^^^ This would favour an activation of plasminogen by UPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The invasion of resealed CAMs required catalytically active plasminogen activator ( PA ) of the urokinase type ( uPA ) ; the invasive efficiency of tumor cells was reduced by 75 % when tumor uPA activity or tumor uPA production was inhibited . ^^^ The mere stimulation of either uPA or tissue plasminogen activator production , in absence of binding to cell receptors , did not result in an enhancement of invasiveness . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Heparin also increases the activity in mixtures of urokinase type plasminogen activator ( uPA ) and plasminogen but has no effect on streptokinase or plasmin . ^^^ Direct analyses of plasminogen activation by polyacrylamide gel electrophoresis demonstrate that heparin increases the activation of plasminogen by both tPA and uPA . ^^^ Binding studies show that heparin binds to various components of the fibrinolytic system , with tight binding demonstrable with tPA , uPA , and Lys plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have carried out enzymatic , immunofluorescence , and surface iodination studies which show that B 16 melanoma cells express the single chain form of the urokinase type plasminogen activator ( uPA ) on their cell surface , and that these cells are capable of plasminogen dependent fibronectin degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is produced and secreted by cultured human keratinocytes as a single chain precursor . ^^^ The synthesis of uPA as a precursor with reduced enzymatic activity as well as decreased affinity for inhibitors is likely to be a mechanism by which normal epidermis regulates plasminogen activation in vivo . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The electrophoretic pattern showed a 48 50 kDa secreted protein identified as urokinase type plasminogen activator ( PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mammalian serine protease zymogen , plasminogen , can be converted into the active enzyme plasmin by vertebrate plasminogen activators urokinase ( uPA ) , tissue plasminogen activator ( tPA ) , factor 12 dependent components , or by bacterial streptokinase . ^^^ Three new thrombolytic agents tPA , pro uPA , and acylated streptokinase plasminogen complex have been found to possess better properties over their predecessors , urokinase and streptokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type of plasminogen activator ( uPA ) is subject to regulation by hormones , phorbol esters and oncogenic transformation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mutants of the pig kidney cell line , LLC PK 1 , affected in cAMP metabolism , were examined for cAMP dependent protein kinase ( cAMP PK ) activity and for cAMP mediated induction of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to LLC PK 1 cells , the mutant did not exhibit production of urokinase type plasminogen activator ( uPA ) in response to the hormones calcitonin and vasopressin , but produced the expected levels of uPA upon stimulation by the receptor independent adenylate cyclase activators forskolin and cholera toxin , as well as by the phosphodiesterase inhibitor isobutylmethylxanthine and the 8 bromo analogue of adenosine cyclic monophosphate , Br8cAMP . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The single chain form of human urokinase plasminogen activator ( uPA ) is the major form of the enzyme found in cells , tissues , and extracellular fluids . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Many human cells and cell lines possess a specific receptor that binds urokinase plasminogen activator ( uPA ) with an affinity of about 10 ( 10 ) M . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previous studies have shown that the region of human urokinase type plasminogen activator ( uPA ) responsible for receptor binding resides in the amino terminal fragment ( ATF , residues 1 135 ) ( Stoppelli , M . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have found that in contrast , mouse granulosa cells produce a different form of plasminogen activator , the urokinase type ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Denuded oocytes collected from ovaries of hypophysectomized , estrogen treated immature rats contained a tissue type plasminogen activator ( tPA ) , but not urokinase ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A single chain urokinase type plasminogen activator ( A431sc uPA ) was purified approximately 18 , 000 fold from A 431 human epidermoid carcinoma cell supernatants by monoclonal antibody immunoaffinity chromatography on 5B4 agarose and ion exchange FPLC ( overall yield 63 % ) . ^^^ SDS PAGE in reducing and non reducing conditions , Western blot analysis and zymography showed that A431sc uPA is a single chain protein of about 50 , 000 Mr immunologically related to urokinase ( uPA ) and distinct from tissue plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) plays a key role in malignant tumor behavior . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We now show that in human fetal fibroblasts UV light enhanced the two mRNA species coding for the urokinase type plasminogen activator ( uPA ) and the tissue type plasminogen activator , but immunological analysis revealed exclusively uPA activity . ^^^ Second , replacement of conditioned medium in irradiated cells while the rate of plasminogen activator induction was maximal rapidly and completely stopped any further increase in uPA activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS PAGE ) followed by a fibrin overlay technique to assess plasminogen activator activity , we observed that treatment with VIP stimulated the secretion of tissue type plasminogen activator ( tPA ) , but not urinary type plasminogen activator ( uPA ) , in a dose dependent manner by cultured granulosa cells as well as by cumulus oocyte complexes , but not by denuded oocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two types of plasminogen activator ( tissue type , tPA ; urokinase type , uPA ) have been demonstrated in ovarian granulosa cells , but only tPA activity was found in denuded oocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Because both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) are secreted by cultured rat granulosa cells , we have examined the activities of these proteins in ovarian homogenates as well as granulosa and theca interstitial ( TI ) cells during gonadotropin induced ovulation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By combining the wounded monolayer model with a substrate overlay technique , we show that cells migrating from the edges of an experimental wound display an increase in urokinase type plasminogen activator ( uPA ) activity , and that this activity reverts to background levels upon cessation of movement , when the wound has closed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two molecular variants of plasminogen activator ( PA ) : urokinase ( uPA ) and tissue type plasminogen activator ( tPA ) , have been reported to be synthesized in the rat testis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The total plasminogen activator ( PA ) activity and the activities of urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators were measured in 43 primary human breast cancer homogenates . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cultures of dissociated neonatal mouse cerebellar cells secrete primarily tissue plasminogen activator ( tPA ) and to a lesser extent urokinase plasminogen activator ( uPA ) into the culture medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cryostat sections of such corneas , taken at various times post scrape , were overlaid with fibrin films containing plasminogen to examine the distribution of PA activity ; and antibodies to tissue plasminogen activator ( tPA ) or to urokinase like activator ( uPA ) were incorporated into the films so that the immunochemical natures of detected PA activities could be determined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cultured human cytotrophoblasts synthesize and secrete urokinase type plasminogen activator ( uPA ) during the first 24 h of culture , but secretion declines during the subsequent day . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In LLC PK 1 pig kidney cells , treatment with a cAMP elevating peptide hormone , calcitonin , induces the accumulation of urokinase type plasminogen activator ( uPA ) mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The peptide hormone calcitonin induces the accumulation of urokinase type plasminogen activator ( uPA ) mRNA in pig kidney LLC PK 1 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of urokinase type plasminogen activator ( uPA ) in response to either reagents activating cAMP dependent protein kinase ( cAMP PK ) or the calcium ion phospholipid dependent kinase ( C kinase ) was compared in the LLC PK 1 and T47D cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The peptide hormone , calcitonin , induces urokinase type plasminogen activator ( uPA ) enzyme activity in cultured LLC PK 1 pig kidney cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This change was accompanied by a reduced production of plasminogen activator ( PA ) of the urokinase type ( uPA ) , which is secreted by HEp 3 cells , a change in response to effectors that modulate uPA production , and an alteration of cell morphology . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor promoting phorbol esters and mezerein strongly induced plasminogen activator ( urokinase , uPA ) synthesis in porcine kidney cell cultures ( LLC PK 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) receptor expression on disseminated tumour cells was significantly correlated with increasing tumour cell counts and clinical prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We found increased 50 kd urokinase like plasminogen activator ( uPA ) and 62 kd gelatinase activity by FTC 238 cells compared with the less invasive FTC 133 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It has been posited that the GPI anchor facilitates clearance of uPAR bound complexes between two chain urokinase ( tcuPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) by the alpha 2 macroglobulin receptor ( alpha 2MR ) which permits re expression of unoccupied uPA receptors on the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We report here that Pg binds to the surface of the human osteosarcoma cell line MG 63 and is activated to Pm by endogenous urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
SMT cc 1 cells have urokinase type plasminogen activator ( uPA ) on their cell surface , and more than half of the cell associated uPA is enzymatically inactive single chain uPA ( pro uPA ) . uPA is bound to a specific surface receptor that is not completely saturated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) is the rapid physiologic inhibitor of tissue type plasminogen activator and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) activity has previously been shown to play a role in migration of cells into basement membranes , and it has been proposed that uPAR also is involved in this process . uPA is known to be internalized and degraded after complex formation with the inhibitor PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They include the urokinase type plasminogen activator ( uPA ) and the metalloproteases , collagenases , gelatinases and stromelysin . uPA secreted from endothelial cells remains associated with the cell membrane , on specific receptors localized in the vicinity of the receptors for plasminogen . ^^^ The degradation of the fibrin clot involves the tissue type plasminogen activator tPA , which like the uPA activates plasminogen to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Because of the potential importance of plasmin generation in these processes , we evaluated the effect of elevated glucose concentrations on expression of plasminogen activator inhibitor 1 ( PAI 1 ) , tissue plasminogen activator ( tPA ) , and urokinase ( uPA ) in cultured bovine brain endothelial cells ( BBEC ) versus cultured bovine aortic endothelial cells ( BAEC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Assays for urokinase plasminogen activator type ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and the urokinase receptor ( uPAR ) were conducted . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Incubation of endothelial cells with the tumor cell conditioned media also results in increased expression of urokinase plasminogen activator ( uPA ) , a key component of the proteolytic system required for cell invasion and capillary formation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The possible involvement of the plasmin cascade in the activation of these proteinases was also explored by examining the presence of the urokinase type plasminogen activator ( uPA ) in aneurysm wall . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The glycosylphosphatidylinositol ( GPI ) anchored membrane protein urokinase plasminogen activator receptor ( uPA R ; CD 87 ) is one of the key molecules involved in migration of leukocytes and tumor cells . uPA bound to uPA R provides the cell proteolytic potential used for degradation of extracellular matrix . uPA R is also involved in induction of cell adhesion and chemotaxis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recently , relative increases in the amounts of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in tumor samples have been correlated with poorer , pathological grade , shorter disease free interval , and shorter survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Northern analysis of poly A+RNA prepared from cultured human mesangial cells revealed mRNA for tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and uPA receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recent studies have shown that elevated levels of urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in breast cancer correlate with an increased risk of a reduced relapse free survival time and shortened overall survival times . ^^^ The fact that plasminogen activators are indispensable for tube formation of microvascular cells and that they may induce angiogenesis in vitro strongly suggests a role for uPA and PAI 1 in tumour neovascularisation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the effects of cAMP on the urokinase type plasminogen activator ( uPA ) production in human pre B lymphoma cell line RC K 8 that is consistently secreting uPA in the conditioned medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activated macrophages secrete urokinase type plasminogen activator ( uPA ) , which mediates the formation of the serine proteinase plasmin from the ubiquitous zymogen plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The distributions of urokinase and tissue plasminogen activators ( uPA , tPA ) , uPA receptor ( uPAR ) , and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) were studied immunohistochemically in two subsets of colorectal adenocarcinomas with low and high aggressiveness , respectively : nine Dukes ' stage A tumors with additional other good prognostic markers and 13 Duke ' s stage C tumors with also other poor prognostic markers ( referred to as Dukes ' stage A and Dukes ' stage C tumors ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase type plasminogen activator ( uPA ) is a proteolytic enzyme thought to be involved in processes leading to tumor cell invasion of surrounding tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Complex formation with target proteases , tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , caused decreased orientational freedom of BDYIA in the P 3 position , while the orientational freedom of BDYIA in position P 1 ' increased to a level similar to that of BDYIA in reactive center cleaved PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to determine whether the T cell lymphokines interleukin 4 ( IL 4 ) and gamma interferon ( gamma IFN ) affect SMC fibrinolysis and migration , we examined the effects of human recombinant IL 4 and gamma IFN on human aortic SMC tissue type plasminogen activator ( TPA ) , urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) antigen production , as determined by enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Experimental measurements of cardiac output , heart rate , tissue plasminogen activator ( TPA ) , urokinase plasminogen activator ( UPA ) , plasminogen activator inhibitor ( PAI 1 ) , C 1 inhibitor , and TPA / C1 inhibitor complex during the infusions and exercise were used to develop a comprehensive fluid phase model of the circulatory regulation of fibrinolysis . alpha and beta adrenergic agonists increased TPA and UPA in plasma by different mechanisms : Phenylephrine decreased hepatic blood flow and thus clearance while isoproterenol stimulated increased secretion of TPA and UPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase type plasminogen activator ( uPAR ) may contribute to the invasive and metastatic capacity of tumor cells by focusing the serine protease urokinase type plasminogen activator ( uPA ) to the cell surface . uPA activates plasminogen to plasmin which in turn degrades extracellular matrix proteins or activates other proteases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
AM also induces the release of antithrombin and urokinase type plasminogen activator from ECs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 2 form a covalent complex that , upon binding to the uPA receptor ( uPA R ) , is cleaved into two fragments of molecular masses 70 kDa and 22 kDa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The presence of mRNA for the uPA receptor ( uPAR ) has been demonstrated in these cells and steady state levels shown to be greatly enhanced , the response being rapid and sustained for at least 24 hours . mRNA for plasminogen activator inhibitor 1 ( PAI 1 ) was modulated in a biphasic manner , with inhibition of the constitutive level apparent at 4 hours of treatment and stimulation apparent at 12 hours and longer , while PAI 1 protein , measured by an ELISA assay for rat PAI 1 , was diminished over this period . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , bound to its receptor ( uPAR ; CD 87 ) facilitates plasmin formation , which promotes matrix proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We quantitatively analyzed PAs of the tissue type ( TPA ) and urokinase type ( UPA ) , PAIs , and plasminogen in protein extracts from different layers of human aorta in relation to the presence and severity of atherosclerotic lesions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vivo and in vitro experimental models have suggested a major role for the urokinase type plasminogen activator ( uPA ) in tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a serine proteinase that has been implicated in the pathogenesis of several epithelial tumors , but its role in HPV associated tumors is not known . ^^^ Both uPA and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , were overexpressed in the HPV 16 transformed cells relative to the parental cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a protease involved in the process of tissue remodelling and cell migration in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) inhibited PA activity of preformed uPA / uPAR complexes and increased cycling of the receptor from the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We describe a cell surface associated pathway of plasminogen activation which crucially depends on cell surface receptors for ( pro ) uPA and plasmin ( ogen ) . uPA bound to its receptor converts cell bound plasminogen into the active protease plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Receptors for urokinase ( uPA ) and plasminogen provide a mechanism to direct the cellular activation of plasminogen . ^^^ In these studies , the effect of transforming growth factor beta 1 ( TGF beta 1 ) on uPA , uPA receptor , and plasminogen receptor expression by human THP 1 macrophage was examined . ^^^ We conclude that TGF beta 1 stimulates membrane bound plasmin activity , without affecting plasminogen receptor expression , through the up regulation of uPA and the uPA receptor expression . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Occupancy of uPAR with urokinase type plasminogen activator further enhanced the cell adhesion to fibrinogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TS 1 expression resulted in a > 100 fold decrease in net fibrinolytic ( urokinase type plasminogen activator , uPA ) activity due to more plasminogen activator inhibitor 1 and less uPA secretion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TGF beta formed within the initial 24 h modulated the plasminogen activator system by down regulating uPA , suggesting that TGF beta temporally modulated its own formation by regulating cell associated uPA . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : The significance of tumor associated proteolysis as reflected by parameters of the urokinase type plasminogen activator ( uPA ) system for prognosis in cancer patients has been proposed because of evidence for its central role in basic mechanisms of invasion and metastasis . ^^^ PATIENTS AND METHODS : In a consecutive series of 203 patients resected for primary gastric cancer , the expression of uPA , uPA receptor ( uPA R ) , plasminogen activator inhibitor ( PAI ) 1 , and PAI 2 was determined immunohistochemically . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) are produced by osteoblasts , as are the specific inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) and a cellular receptor for uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase type plasminogen activator ( uPAR ) is an integral membrane protein that specifically binds urokinase type plasminogen activator ( uPA ) and plays a crucial role in cell surface plasmin generation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In developing rabbit brain we studied expression of metalloproteinases ( MMP ) 1 and 3 by in situ hybridization and MMP 2 and tissue and urokinase type plasminogen activators ( tPA and uPA ) by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cellular regulatory sequences responsive to the c Ets 1 proteins include a urokinase type plasminogen activator ( uPA ) gene enhancer , the stromelysin 1 and the collagenase 1 gene promoters . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of the plasminogen activation system demonstrates that Co 112 cells express the urinary type plasminogen activator ( uPA ) and Co 115 cells the tissue type ( tPA ) , exclusively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytic degradation processes , resulting from the activation of plasminogen by the specific enzyme , urokinase plasminogen activator ( uPA ) , take place in the extracellular matrix during cancer invasion as well as in tissue remodelling under certain normal physiological conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The relevance of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor ( PAI ) type 1 in predicting the survival probability of patients with advanced ovarian cancer after radical surgery and adjuvant chemotherapy by assessing the patients ' primary tumors has recently been shown by us ( W . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : The urokinase type plasminogen activator receptor ( u PAR ) contributes to colon cancer invasion and metastases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Butyrate was found to induce a rapid and transient increase in plasminogen activator inhibitor type 1 ( PAI 1 ) mRNA while concomitantly suppressing the constitutive production of both urokinase type plasminogen activator ( uPA ) and uPA receptor ( uPAR ) mRNA transcripts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In previous work we suggested that a kidney specific transcription factor LFB 3 cooperates with cAMP response element ( CRE ) binding proteins within a cAMP regulatory unit comprised of three protein binding domains and located 3 . 4 kilobase pairs upstream of the urokinase type plasminogen activator ( uPA ) gene in LLC PK 1 cells ( Menoud , P . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression and function of urokinase plasminogen activator ( uPA ) , an extracellular protease , were examined in four established prostate cancer lines , and one uPA transfected cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using type 2 pneumocytes , endocytosis of a previously described gp 330 ligand , urokinase ( uPA ) complexed with plasminogen activator inhibitor 1 ( uPA : PAI 1 ) and two new ligands , PAI 1 and pro uPA , was demonstrated . ^^^ In addition , gp 330 acts in concert with LRP in type 2 pneumocytes to mediate clearance of a variety of proteins involved in plasminogen activation , including uPA : PAI 1 complexes PAI 1 and pro uPA . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor necrosis factor ( TNF ) can promote endothelial cell transcription , synthesis , and secretion of urokinase plasminogen activator ( uPA ) augmenting extracellular matrix remodeling and influencing cellular differentiation . ^^^ Analysis of cell free conditioned medium obtained from PKC inhibitor treated cultures by micro enzyme linked immunosorbent assay methodologies using uPA and plasminogen activator inhibitor type 1 ( PAI 1 ) specific monoclonal antibodies indicate that the decrease in uPA activity observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis zymography was a direct result of decreased extracellular uPA antigen and not a consequence of increased PAI 1 antigen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Macrophage expression of urokinase type plasminogen activator ( uPA ) appears to play a role in their release of matrix bound basic fibroblast growth factor ( bFGF ) and transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This effect is limited to certain genes only as the concentration of the 92kD collagenase m RNA and uPA ( urokinase type plasminogen activator ) m RNA , which are both produced in cytotrophoblasts to a much higher extent than in JAr cells , and are not increased by cultivating the cytotrophoblasts with JAr cells in co culture . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The relative topographical distribution of urokinase type plasminogen activator ( uPA ) , tissue type PA ( tPA ) , PA inhibitor 1 ( PAI 1 ) , PA inhibitor 2 ( PAI 2 ) , plasmin ( ogen ) , alpha 2 antiplasmin , and alpha 2 macroglobulin was studied in lesional epidermis of psoriasis vulgaris , and in normal epidermis , by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Test for urokinase type plasminogen activator inhibitor of edible plants in vitro ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) gene transcription is increased > or = 50 fold in chicken embryo fibroblasts ( CEF ) following transformation by the protein tyrosine kinase pp60v src . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previous work has shown that LRP is responsible for mediating the internalization of urinary type plasminogen activator ( uPA ) complexed to plasminogen activator inhibitor type 1 ( PAI 1 ) ( Nykjaer et al . , 1992 ; Herz et al . , 1992 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Because mitogens such as basic fibroblast growth factor ( bFGF ) and platelet derived growth factor ( PDGF ) are positive regulatory factors and heparin is a negative regulatory factor for smooth muscle cell proliferation and migration , we have looked at the effects of these factors ( plus serum and phorbol ester ) on urokinase ( uPA ) and tissue plasminogen activator ( tPA ) of smooth muscle cells . ^^^ These data demonstrate that PDGF , bFGF and serum can differentially regulate smooth muscle cell plasminogen activators and that heparin can either increase or decrease levels of tPA or shift the localization of uPA depending on the mitogen used . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The promoter of the human urokinase plasminogen activator ( uPA ) gene contains a sequence identical with the retinoblastoma control element ( RCE ) of the murine c fos gene , as well as several Sp 1 binding sites . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It is now clearly established that proteolytic enzymes , and in particular plasminogen activator ( uPA ) , play an important role in breaking down the extracellular matrix , which is considered to be a step in metastasis formation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytic enzymes such as urokinase type plasminogen activator ( uPA ) , plasmin , and collagenase mediate proteolysis by a variety of tumor cells . uPA secreted by tumor cells can be bound to a cell surface receptor via a growth factor like domain within the amino terminal fragment ( ATF ) of the uPA molecule with high affinity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Therefore , we assessed the effects of RU 486 administration on the expression of immunoreactive ( ir ) endometrial stromal cell urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activator and their activities as well as levels of ir type 1 plasminogen activator inhibitor ( PAI 1 ) using a well characterized in vitro model of decidualization . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) interacts with urokinase type plasminogen activator ( uPA ) via tPA ' s lysine binding site . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of the plasminogen activators ( PAs ) , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thus , caveolae promote efficient cell surface plasminogen activation by clustering uPAR , uPA , and possibly other protease receptors in one membrane compartment . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mutagenesis at Pro 309 of single chain urokinase type plasminogen activator alters its catalytic properties . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) is a potent angiogenic factor and endothelial cell specific mitogen that stimulates urokinase type plasminogen activator ( uPA ) activity in vascular endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) is a proteolytic enzyme which converts the proenzyme plasminogen to the active serine protease plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Oxidation of LA by lipoxidase especially increases tumour cell death , whilst GLA inhibits urokinase type plasminogen activator ( uPA ) activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , the expression of matrix metalloproteinase gelatinase B ( MMP 9 ) , urokinase type plasminogen activator ( uPA ) and their inhibitors was investigated during early mouse embryo development . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , although the catalytic domain of human urokinase type plasminogen activator ( uPA ) has 40 % identity to bovine trypsin and the substrate specificities of these two proteases are virtually identical , ecotin inhibits uPA almost 10 , 000 fold less efficiently than trypsin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One of the plasminogen activators , urokinase type plasminogen activator ( uPA ) , binds to a specific surface and provides a localized cell surface proteolytic activity required for the destruction of extracellular matrix , which is a vital step in tumor cell invasion . ^^^ The proteolytic activity of uPA is modulated by its cell surface receptor , as well as by plasminogen activator inhibitor type 1 ( PAI 1 ) and , to a lesser degree , by other inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The levels of plasminogen activators ( uPA and tPA ) and their inhibitors ( PNI and PAI 1 ) were determined by fibrin zymography , ELISA , amidolytic activity assay , complex formation , and Western blot analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a proteolytic enzyme involved in degradation of the extracellular matrix during cancer invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activity of receptor bound urokinase plasminogen activator ( uPA ) on the surface of colon cancer cells appears to be a function of the number of uPA receptors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , B . burgdorferi bound human plasmin , plasminogen ( Pgn ) , and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An increased expression of urokinase type plasminogen activator ( uPA ) is of significance for degradation of the extracellular tumor matrix , facilitating invasiveness and growth . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mouse urokinase type plasminogen activator ( uPA ) gene was used as a model macrophage colony stimulating factor 1 ( CSF 1 ) inducible gene to investigate CSF 1 signalling pathways . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HGF is produced in an inactive single chain form that can be cleaved in vitro to the active two chain form by tissue type and urokinase type plasminogen ( PLG ) activators ( tPA and uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease involved in cancer invasion and metastasis . uPA mediates its action while attached to a membrane bound receptor ( uPAR ) . ^^^ In this investigation we show that uPAR levels correlate with uPA levels in human breast cancers . uPAR levels , however , do not correlate with other components of the plasminogen activator system such as tissue type plasminogen activator ( t PA ) , PAI 1 or PAI 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We evaluated urokinase type plasminogen activator ( uPA ) expression with immunohistochemistry in 90 cases of colorectal cancer and compared it with the clinicopathological findings . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Additionally , we have measured the release of urokinase ( uPA ) , tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) into the cell culture media . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recent experiments show that both LIF and EGF stimulate secretion of urokinase type plasminogen activator ( uPA ) and gelatinase B / matrix metalloproteinase 9 ( MMP 9 ) in day 7 mouse blastocyst outgrowths . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase plasminogen activator ( uPA R , CD 87 ) is a glycosylphosphatidylinositol ( GPI ) anchored 50 to 65 kD glycoprotein that , by regulating membrane associated plasmin activity , may facilitate the invasion of inflammatory and malignant cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrinolytic activity was plasminogen dependent and due to both urokinase ( uPA ) and tissue plasminogen activator ( tPA ) . ^^^ Conversely , TNF alpha increased plasminogen activator ( PA ) activity due to increased uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expressions of urokinase ( uPA ) and tissue type plasminogen activators ( tPA ) in different stages of the rat seminiferous epithelial cycle were analyzed by in situ and Northern hybridizations combined with zymographic analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Qualitative histological studies in the distribution of urokinase type plasminogen activator ( uPA ) in human colorectal carcinomas have been well documented . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , polymerized ampicillin inhibits the activation of plasminogen by either urokinase like plasminogen activator ( uPA ) or tissue type plasminogen activator ( tPA ) . ^^^ At 7 . 5 mM of polymerized ampicillin , the uPA mediated plasminogen activation is suppressed by 94 % , and half maximal inhibition is obtained at 0 . 66 mM . ^^^ The inhibitory effects of the polymerized antibiotic on the activation of plasminogen by both uPA and tPA is totally abolished in presence of fibrin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The tumor associated protease urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 were detected in malignant ovarian cancer tissue extracts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , which binds to cells via a specific receptor ( uPAR ) , participates in pericellular proteolysis during leukocyte migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inactivation of receptor bound pro urokinase type plasminogen activator ( pro uPA ) by thrombin and thrombin / thrombomodulin complex . ^^^ Urokinase type plasminogen activator ( uPA ) binds with high affinity to a specific cell surface glycosyl phosphatidyl inositol ( GPI ) anchored receptor , the urokinase receptor ( uPAR ) . ^^^ Pro uPA , the enzymatically inactive single chain form of uPA after having been activated by certain proteases , converts plasminogen into plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
KW cells stained ( a ) positive for vimentin , smooth muscle specific alpha actin , tissue type plasminogen activator ( tPA ) , urokinase type PA ( uPA ) , uPA receptor , PA inhibitor 1 , latent transforming growth factor beta 1 ( latent TGF beta 1 ) and 17 beta hydroxysteroid dehydrogenase type 1 , and ( b ) negative for desmin , endoglin and cytokeratin 19 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mechanism of induction of urokinase type plasminogen activator ( uPA ) by basic fibroblast growth factor ( bFGF ) was explored in fetal bovine aortic endothelial GM 7373 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In situ hybridization documented the uniform expression of insulin like growth factor 1 ( IGF 1 ) and of urokinase type plasminogen activator ( uPA ) mRNA in MG 63 and PC 3 cells separately cultured in this substrata . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effects of butyrate on the modulation of urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) mRNAs were studied . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The antigen levels of urokinase type plasminogen activator ( uPA ) and its inhibitor plasminogen activator inhibitor ( PAI ) 1 , as detected in tumor extracts by ELISA , have been reported to be correlated with a poor prognosis in primary breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
During macrophage activation , CSF 1 promotes urokinase type plasminogen activator ( uPA ) activity ; in macrophages and in malignant cells of lung , breast , colon , and prostatic origin , uPA activity is strongly correlated with the ability to invade and , in the malignant cells , to metastasize . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 2 ( PAI 2 ) production by tumor cells was enhanced by suramin ( 100 micrograms / ml ) , whereas urokinase type plasminogen activator ( uPA ) production was suppressed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Complex formation with tissue or urokinase type plasminogen activator ( tPA or uPA ) , and cleavage between P 3 and P 4 with a catalytic concentration of elastase , all resulted in identical 13 nm blue shifts of the peak fluorescence emission wavelength and 6 . 2 fold fluorescence enhancements . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type ( tPA ) and urokinase type ( uPA ) plasminogen activator activity was assessed using chromogenic substrate assays , while smooth muscle cell proliferation and activation was monitored using expression of proliferating cell nuclear antigen ( PCNA ) and of basic fibroblast growth factor ( bFGF ) respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The inhibition of migration by HKa is reversed by antibodies to urokinase type plasminogen activator receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Radiolabeled pro urokinase plasminogen activator ( pro uPA ) was cross linked to a specific protein on the surface of human monocyte like U 937 cells in a reaction catalyzed by tissue transglutaminase . ^^^ The conjugate formed with this unknown component had a much higher molecular weight ( apparent M ( r ) 250 , 000 300 , 000 ) than the complex of pro uPA and the urokinase plasminogen activator receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TNF alpha treatment of human umbilical vein endothelial ( HUVE ) cells induced urokinase type plasminogen ( uPA ) activity , increased HUVE uPA dependent extracellular matrix ( ECM ) degradation , and accelerated matrix remodeling and endothelial differentiation into tubes or cord like structures . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase type plasminogen activator ( uPA R ) localizes uPA to the cell surface . ^^^ The receptor bound uPA converts plasminogen to the trypsin like endopeptidase plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effects of calcium on human foreskin keratinocyte expression of urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activator enzymes and plasminogen activator inhibitor 1 and 2 ( PAI 1 , PAI 2 ) were assessed by Northern analyses . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have compared the histological localization of urokinase plasminogen activator ( uPA ) and urokinase plasminogen activator receptor ( uPAR ) in breast cancer sections using a panel of monoclonal antibodies . ^^^ First , the ability of six different anti uPA monoclonal antibodies to recognize pro uPA , uPA , and in vitro formed complexes of uPA with either soluble uPAR or with plasminogen activator inhibitor type 1 was compared . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The binding of urokinase type plasminogen activator ( uPA ) to its receptor ( uPAR ) on cell surfaces has the potential to influence degradation of extracellular matrix ( ECM ) . ^^^ Thus , uPA bound to monocyte / macrophages and its interactions with plasminogen activator inhibitors types 1 and 2 ( PAI 1 and PAI 2 ) may modify atherogenesis by altering cell associated proteolytic activity , degradation of ECM , and neointimal formation at sites of vascular injury . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TaqI and PvuII restriction fragment length polymorphisms at the porcine plasminogen activator , urokinase , locus ( PLAU ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) but not urokinase plasminogen activator ( u PA ) was found in tears . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Aged rats had lower plasma levels of tissue type plasminogen activator ( t PA ) and of urokinase type PA ( u PA ) activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transfectants produce a urokinase plasminogen activator ( u PA ) binding protein that is secreted into the medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Monocytes and monocytoid cell lines previously have been shown to express receptors for plasminogen and urokinase ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here we describe the receptor for urokinase type plasminogen activator ( uPA R ) in the human keratinocyte cell line HaCaT , which serves to direct plasminogen activation to the cell surface ; we relate the receptor to the uPA R previously described in human myelo / monocytes . ^^^ Binding of uPA to the receptor accelerated plasminogen activation by a factor of approximately 10 , compared to uPA in solution . ^^^ Receptor bound uPA was susceptible to inhibition by the plasminogen activator inhibitors 1 and 2 . uPA and uPA R antigen , as well as uPA activity , were localized to the leading front of expanding sheets of HaCaT cells . ^^^ Our findings support the hypothesis that uPA mediated plasminogen activation is characteristic of mobile rather than sessile keratinocytes . ^^^ Moreover , the uPA R seems to focalize plasminogen activation to the surface of cells at the site of keratinocyte migration . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) assayed in uteri of cycling and deciduoma bearing rats shows detectable levels of this enzyme in endometrium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It was recently found that urokinase type plasminogen activator ( uPA ) is involved in the cleavage of its receptor ( uPAR ) on cultured cells , thereby releasing one of the receptor ' s 3 domains ( the ligand binding domain 1 ) and leaving the 2 others [ uPAR ( 2 + 3 ) ] anchored to the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The alpha 2 macroglobulin receptor / low density lipoprotein receptor related protein ( alpha 2MR / LRP ) binds several ligands , including complex between the two chain urokinase type plasminogen activator ( uPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) , and the single chain zymogen pro urokinase ( pro uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To assess the mode of cancerous invasion of this carcinoma , we examined the urokinase type plasminogen activator ( uPA ) activity of both types of cells ( cancer cells and fibroblasts ) with a coculture method . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and its type 1 inhibitor ( PAI 1 ) : regulators of proteolysis during cancer invasion and prognostic parameters in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In cell homogenates PAI 2 was probed by [ 125I ] urokinase plasminogen activator ( uPA ) and detected as a sodium dodecyl sulphate stable M ( r ) 80 , 000 complex after reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The activity of uPA on the cell surface appears to be a function of the number of uPA specific receptors ( uPAR ) and the extent of inhibition of uPA by plasminogen activator inhibitors ( PAI ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TNF alpha increased the steady state level of urokinase type plasminogen activator ( uPA ) , and NAC inhibited this increase at a dose that also inhibited the increase in the intracellular oxidation state . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) from artificially induced decidual tissue of rat has been purified to homogeneity employing chromatographic techniques and the final preparation has a specific activity of 12 , 084 I . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present study was undertaken to assess the role of tumor associated urokinase type plasminogen activator ( uPA ) and its inhibitor type 1 ( PAI 1 ) as a predictor for early relapse and poor prognosis in patients with stage 2 cervical cancer of the uterus . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Receptors for urokinase type plasminogen activator ( uPAR ) are present on the surface of many cell types and appear to be the key determinant controlling extracellular proteolysis catalyzed by the urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enzyme linked immunosorbent assay of urokinase type plasminogen activator ( uPA ) in cytosolic extracts of human breast cancer tissue . ^^^ The enzyme urokinase type plasminogen activator ( uPA ) plays a role in cancer invasion , and high levels of uPA in detergent extracts of mammary cancer tissue have been reported to be associated with a poor prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Coculture of the human squamous cell carcinoma cell line II 4 with primary human foreskin fibroblasts was observed to induce mRNA expression of urokinase type plasminogen activator ( uPa ) , matrilysin , 92 kDa type 4 collagenase , and c ets , a transcriptional activator of several genes within the serine and matrix metalloprotease families . uPA and c ets induction were localized to the fibroblast cell population . uPa induction was found to be dependent upon cell cell contact with the tumor cell population , whereas c ets induction was due to a combination of cell cell contact and a tumor cell derived soluble factor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
After binding to its receptor ( uPAR ) , active cell surface urokinase ( uPA ) is not internalized while the complex formed by uPA with plasminogen activator inhibitor type 1 ( PAI 1 ) is internalized and degraded . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We examined the relative amounts of mRNA and protein and localization of urokinase type plasminogen activator ( uPA ) in human astrocytomas in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Northern blot analysis demonstrated expression of urokinase type plasminogen activator ( uPA ) , tissue type PA ( tPA ) and PA inhibitor 1 ( PAI 1 ) in some of the above cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : Evaluation of the relationship between plasminogen activator inhibitor 1 ( PAI 1 ) and the metastatic potential of primary breast cancer , and to compare the prognostic impact of PAI 1 in multivariate analysis with those of conventional prognostic factors , including steroid hormone receptors , and those of urokinase plasminogen activator ( uPA ) , pS 2 protein ( PS 2 ) , and cathepsin D . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This protein is composed of three homologous domains , the NH 2 terminal of which is involved in the high affinity binding ( Kd approximately 0 . 1 1 . 0 nM ) to the epidermal growth factor like module of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We found that urokinase type plasminogen activators ( uPA ) and plasminogen activator inhibitor type 1 mRNAs accumulate in atypical nevocytes and in melanoma cells , but not in benign nevocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mononuclear phagocytes concentrate urokinase type plasminogen activator ( uPA ) at the cell surface by expressing membrane uPA receptors ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation is regulated by the interaction between urokinase type plasminogen activator ( uPA ) and its specific glycolipid anchored cell surface receptor ( uPAR ) . uPAR is composed of three homologous domains and is the only multi domain member of the Ly 6 family of glycolipid anchored membrane proteins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A selective inhibitory antibody , raised against human high molecular weight urokinase type plasminogen activator ( HMW uPA ) , was examined to determine whether it would inhibit production of experimental and spontaneous lung metastasis by murine Lewis lung carcinoma ( 3LL ) cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We assayed urokinase plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) in 43 human brain tumors ( predominantly astrocytic gliomas ) and in histologically disease free brain tissue resected with 21 of the tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we discovered and examined a new function of CPB 1 : promotion of both the migration and the urokinase type plasminogen activator ( uPA ) activity of normal human keratinocytes ( NHK ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have studied the prognostic value of urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , and type 1 plasminogen activator inhibitor ( PAI 1 ) in tumor extracts from 84 patients with squamous cell lung carcinoma and 38 patients with large cell lung carcinoma , measuring each molecule with sandwich enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is initially produced by cells as a single chain precursor ( pro uPA ) , which can be cleaved by the serine protease plasmin to form the two chain molecule uPA . ^^^ Cell surface binding of both pro uPA and plasminogen is known to enhance the rate of plasminogen activation . ^^^ This study directly demonstrates the enhancement by cells of this feedback activation loop by showing that uPA is generated more rapidly from pro uPA and plasminogen in the presence of human promonocytic U 937 cells than it is under fluid phase conditions . ^^^ Moreover , the enhanced activation of pro uPA and plasminogen observed in the presence of cells was significantly less susceptible to inhibition by alpha 2 antiplasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activity related to tissue plasminogen activator ( t PA ) or urokinase ( u PA ) was quantified by antiserum inhibition . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A significant increase in urokinase type plasminogen activator antigen ( u PA : Ag ) was found only in controls after VO . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present study determined the time course of expression of the genes encoding tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) during the preimplantation period in rats by the sensitive mRNA phenotyping procedure of reverse transcription PCR . ^^^ Most plasminogen activator activity present in preimplantation embryos appeared to be uPA , as it could be inhibited by anti uPA antibody and a specific uPA inhibitor , amiloride , but not by anti tPA antibody . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cRel RelA and NF kappa B ( p 50 RelA ) transcription factors bind to a kappa B like sequence termed Rel related proteins binding element localized in the regulatory region of the human urokinase plasminogen activator ( uPA ) gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Concomitant expression of urokinase type plasminogen activator ( uPA ) and its surface receptor ( uPAR ) has been shown to correlate strongly with a more invasive tumor cell phenotype . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast , HRMEC produced urokinase type plasminogen activator ( uPA ) in a 24 fold excess to PAI 1 and were thereby profibrinolytic with regard to fibrinolysis antigen expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Arterial and coronary sinus blood were sampled concomitantly before cardioplegia and after release of the aortic cross clamp , for measurement of t PA antigen ( Ag ) and activity , plasminogen activator inhibitor ( PAI 1 ) Ag and activity , t PA / PAI 1 complex , single chain urokinase ( sc uPA ) and urokinase ( uPA ) plasminogen activators , the fibrin split product D dimer , thrombin antithrombin complex ( TAT ) , and the prothrombin split product F 1 + 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Efficient synthesis of structurally novel 4 substituted benzo [ b ] thiophene 2 carboxamidines 1 3 , which selectively inhibit urokinase type plasminogen activator ( uPA ) with IC 50 values of 70 320 nM , are described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PAI 1 , which is a natural inhibitor of tumor cell associated urokinase type plasminogen activator ( uPA ) activity , inhibited activation of plasminogen to plasmin in the growth media , thereby preventing plasmin induced detachment of cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Approximately 40 % of the PAI 1 secreted is biologically active as judged by the formation of higher molecular weight , SDS resistant complexes with urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mRNA level for u PA , a plasminogen activator that is inhibited by PAI 2 , was not altered following TCDD treatment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A hybrid plasminogen activator binds to the u PA receptor and has a reduced thrombolytic potency in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator urokinase ( u PA ) mediates proteolysis by a variety of human tumor cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activated by u PA gives plasmin , which is known to further activate pro stromelysin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recent studies have established that cultured human umbilical vein endothelial cells ( ECs ) express receptors for plasminogen ( plg ) and urokinase like plasminogen activator ( uPA ) . ^^^ The retractive effect of plg uPA is concomitant to surface activation of plasminogen and to the loss of cell cell activation of plg can induce EC retraction , possibly by causing proteolysis at specific cell cell contacts and cell matrix sites . ^^^ In these cells , addition of plg and uPA to confluent EC generates a retraction of the monolayer that is evidenced by loss of cell cell contacts and increase in monolayer permeability . ^^^ The phenomenon is reversible even after 6 hours of plg uPA treatment . ^^^ Inhibition of plg uPA effect is obtained with plasmin inhibitors , as well as reagents that block binding of uPA or plg to the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activators , tissue type and urokinase type ( tPA and uPA , respectively ) have been identified in human skin under normal conditions and in various inflammatory dermatoses , including psoriasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of receptor bound urokinase type plasminogen activator ( uPA ) in cellular activation of latent transforming growth factor beta ( LTGF beta ) was investigated in a model system of mouse LB 6 cells transfected with either a human uPA receptor cDNA ( LhuPAR+ ) , a human prouPA cDNA ( LhuPA ) , or a control neomycin resistance cDNA ( Lneo ) . ^^^ Addition of the amino terminal fragment of uPA ( ATF ) or omission of plasminogen from co cultures blocked both the fibrinolytic activity and the generation of TGF beta activity in the CM . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present study was undertaken to assess the role of tumor infiltrating lymphocytes and polymorphonuclear leukocyte derived human leukocyte elastase ( HLE ) as an inactivator of urokinase type plasminogen activator ( uPA ) activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In LLC PK 1 cells , the urokinase type plasminogen activator ( uPA ) gene is induced by two of the major signal transduction pathways , the protein kinase C ( PKC ) and the cAMP dependent protein kinase ( PKA ) pathways . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The two types of plasminogen activator , tissue type ( tPA ) and urokinase type ( uPA ) , are produced by osteoblasts , as is the specific PA inhibitor , PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cellular receptor for urokinase plasminogen activator ( uPA R ) is a monomeric phosphatidylinositol linked glycoprotein ( gp 40 65 ) that may contribute to the invasive capacity of tumor and inflammatory cells by focusing the activity of urokinase ( uPA ) in converting plasminogen to plasmin , a serine protease capable of degrading extracellular matrix proteins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It was previously found that certain proteases , specifically urokinase like plasminogen activator ( uPA ) and others , required activation in order to effect regeneration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As a response to u PA synthesis surrounding cells synthetize inhibitors of plasminogen activators for tissue protection . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor are instrumental in cell invasion and metastasis ; their high levels of expression in human tumours correlates with a high risk of recurrence . uPA has a pleiotropic effect on cell migration and spreading in vivo and in vitro through the activation of plasminogen or other protein factors at the cell surface or in the extracellular matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , a member of the serine protease inhibitor ( Serpin ) superfamily , is the primary inhibitor of the plasminogen activators tPA and uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The high uPA activity was counteracted both in the growth medium and at the cell surface by an increased plasminogen activator inhibitor ( PAI ) production and reduction of uPA synthesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , recombinant M1Q bFGF showed a drastically reduced capacity to induce the production of urokinase type plasminogen activator ( uPA ) in endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) converts the proenzyme plasminogen to plasmin and thereby contributes to processes like cell migration , tissue remodeling , and cytokine processing . ^^^ This effect was not seen in the absence of plasminogen , was prevented by inhibitors of uPA and plasmin , and was accompanied by changes in the electrophoretic mobility of rIFN gamma on polyacrylamide gels , consistent with limited proteolysis of the lymphokine . ^^^ The ability of rIFN gamma to induce Fc receptors on U 937 cells could also be prevented by providing the cells with a source of exogenous plasminogen , indicating that U 937 cells could control their own activation in vitro through the action of uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The tissue ( tPA ) and urokinase ( uPA ) types of plasminogen activator and the plasminogen activator inhibitor 1 ( PAI 1 ) are enzymatic proteins that may play an important role in the degradation of the extracellular matrix in physiologic and neoplastic conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transgenic mice designated alpha MUPA overproduce in the brain murine urokinase type plasminogen activator ( uPA ) , an extracellular protease implicated in tissue remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mononuclear phagocytes ( Mphi ) produce urokinase type plasminogen activator ( uPA ) and also express a specific cell surface receptor for urokinase , uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recent investigations have shown that in the murine kidney urokinase ( uPA ) and tissue type plasminogen activator ( tPA ) are synthesized and released in urine by tubular epithelial cells , raising the possibility that plasminogen activators ( PAs ) may be involved in the maintenance of patency and fluidity in renal tubules . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The principal activator of plasminogen in tissues is urokinase plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Study has been extended to plasminogen activators ( t PA and u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since the serine protease inhibitor , protein C inhibitor ( PCI ) , is present in seminal plasma at approximately 3 microM , complexes of PCI with urokinase ( uPA ) and tissue type ( tPA ) plasminogen activator were quantitated using sandwich enzyme linked immunosorbent assays ( ELISA ' s ) . ^^^ Seminal plasma ( N = 10 ) collected in the absence of extrinsic inhibitors had a mean of 25 + / 5 ng / ml uPA : PCI , 76 + / 23 ng / ml tPA : PCI , and 4 + / 2 ng / ml of tPA complexes with plasminogen activator inhibitor 1 ( tPA : PAI 1 ) . 93 % of the uPA and 17 % of the tPA antigen in seminal plasma was in complex with PCI and , when complexation was inhibited by collecting semen into an 1 , 10 phenanthrolinium solution , 33 % of the uPA and 7 % of the tPA was complexed to PCI . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator ( uPA ) plays a key role in tumor associated proteolysis in malignant solid tumors . ^^^ Proteolytic activity of uPA is controlled by its naturally occurring plasminogen activator inhibitor type 1 . ^^^ In addition , not only uPA but also plasminogen activator inhibitor type 1 were shown to be of prognostic value in breast cancer ( Jnicke et al . , Semin . ^^^ Subsequently , the prognostic value of uPA and plasminogen activator inhibitor type 1 was also confirmed in studies using archived `` cytosol fractions ' ' of breast cancer tissues ( Foekens et al . , Cancer Res . , 52 : 6101 6105 , 1992 ; Spyratos et al . , J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HOC 1 cells showed detectable and reproducible levels of expression of surface urokinase type plasminogen activator ( uPA ) and plasminogen / plasmin by cell ELISAs and enzyme assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of endothelial cell ( EC ) plasminogen activator inhibitor type 1 ( PAI 1 ) , the primary physiological inhibitor of tissue type plasminogen activator ( TPA ) and urokinase type plasminogen activator ( UPA ) , by various stimuli has been well characterized . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Together with previous findings of urokinase plasminogen activator ( uPA ) protein and mRNA being expressed in tumour infiltrating fibroblast like cells at the invasive foci , these results support the view that the uPA pathway of plasminogen activation is involved in tissue degradation in colon cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition of these enzymatic bands with specific antibodies against tissue type plasminogen activator ( tPA ) and amiloride , an inhibitor for urokinase plasminogen activator ( uPA ) , confirmed that these bands were tPA and uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The prognostic impact of the proteolytic factors urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) was evaluated in 76 completely resected gastric cancer patients enrolled in a prospective study . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thus , u PA seems to play a major role in the endogenous plasminogen activator system following focal cerebral ischemia . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and urokinase plasminogen activator ( u PA ) ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previously , we reported that urokinase type plasminogen activator ( uPA ) plays a pivotal role in extracellular matrix dissolution by malignant melanoma cells . ^^^ This contention is based on the finding that an antibody against uPAR ( monoclonal antibody 3936 ) inhibited invasion of M24met cells through a reconstituted basement membrane ( Matrigel ) up to 33 % , while a reduction of uPA catalytic activity by its plasminogen activator inhibitor 2 resulted in 46 % inhibition of invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There is a significant correlation between enhanced expression of CAPL and presence of the invasivity marker urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Four synthetic peptides ( residues 20 30 and 17 34 ) within the growth factor like domain ( GFD ) of murine and human urokinase type plasminogen activator ( uPA ) were examined to determine whether they inhibit production of experimental and spontaneous lung metastasis by murine Lewis lung carcinoma ( 3LL ) cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin is a fibrinolytic proteinase and is generated from ubiquitous plasminogen by cell derived urokinase type ( uPA ) or tissue type ( tPA ) plasminogen activator . ^^^ The findings point at the importance of monocytes / macrophages and fibroblasts in uPA mediated plasminogen activation in healing human skin wounds . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied the effects of thrombin on the expression of urokinase type plasminogen activator ( uPA ) which is known to play a role in tumor invasion , using a human prostate cancer cell line PC 3 . ^^^ Thrombin did not affect the expression of other components of the plasminogen activation system , tissue type plasminogen activator and type 1 plasminogen activator inhibitor , and uPA receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Renal epithelial cells LLC PK 1 secrete high amounts of urokinase type plasminogen activator ( uPA ) when stimulated through the cAMP signaling pathway . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As determined by histopathological methods , the malignant tumors showed statistically significantly higher expression of urokinase plasminogen activator ( uPA ) , type 1 plasminogen activator inhibitor ( PAI 1 ) , and especially urokinase plasminogen activator receptor ( uPAR ) than benign tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have identified a soluble form of the human urokinase plasminogen activator ( uPA ) receptor ( uPAR ) in the ascitic fluids from patients with ovarian cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Synthetic bPTH ( 1 34 ) increased the activity of tissue type ( tPA ) and urokinase type ( uPA ) plasminogen activators ( PA ) in cell lysates ( CL ) of UMR 106 01 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There are two plasminogen activators ( PAs ) , urokinase type PA ( u PA ) and tissue type PA ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is involved in generating the proteolytic activity necessary for invasive processes , and is dependent on binding to its specific cellular receptor ( uPAR ) for efficient function . ^^^ This inhibition was found to be due to effects on the activation of both pro uPA and plasminogen . ^^^ Similarly , its effect on plasminogen activation was not due to a direct inhibition of uPA . ^^^ The inhibition of plasminogen activation , which occurred exclusively with receptor bound uPA , appeared to be due to a reversal of the favourable kinetics which result from the activation of cell associated plasminogen , although suramin did not inhibit the cellular binding of 125I labelled plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have studied the prognostic value of urokinase type plasminogen activator ( uPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) in tumor extracts from 106 patients with adenocarcinoma of the lung . uPA and PAI 1 levels were determined by sandwich enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Similar inhibitory effects of hCG on urokinase plasminogen activator ( uPA ) and the activity of trophoblast conditioned media were shown ( measured by degradation of S 2444 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Northern blot analysis of EGF supplemented cells revealed an increase in expression of urokinase plasminogen activator ( uPA ) RNA , a serine protease involved in the regulation of pericellular proteolysis and membrane degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prognostic value of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitors PAI 1 and PAI 2 in breast carcinomas . ^^^ It is now clearly established that proteolytic enzymes , including plasminogen activator ( uPA ) , play an important role in breaking down the extracellular matrix , which is considered to be a step in metastasis formation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cellular receptor for urokinase type plasminogen activator ( uPAR ) binds pro urokinase ( pro uPA ) and facilitates its conversion to enzymatically active urokinase ( uPA ) . uPA in turn activates surface bound plasminogen to plasmin , a process of presumed importance for a number of biologic processes including cell migration and resolution of thrombi . ^^^ This process is accompanied by an increased cell associated plasminogen activation after preincubation of neutrophils with pro uPA in vitro . ^^^ Flow cytometry of neutrophils saturated with exogenous diisopropyl fluorophosphate uPA shows a large excess ( approximately 90 % ) of unoccupied uPAR on resting as well as FMLP and PMA stimulated neutrophils , suggesting a possible role for exogenous pro uPA in providing neutrophils with a potential for plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown that the tumor promoter okadaic acid ( OA ) , an inhibitor of protein phosphatases 1 and 2A , transcriptionally induces the urokinase type plasminogen activator ( uPA ) gene in LLC PK 1 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we determined the plasma levels of tissue plasminogen activator ( tPA ) , plasminogen activator inhibitor ( PAI ) , urokinase plasminogen activator ( uPA ) , and von Willebrand factor ( vWF ) antigen in Blackfoot disease patients , in comparison with normal controls from non endemic areas and the endemic area , Putai . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Moreover , harvests from organ cultures of ulcerating corneas not only had higher levels of urokinase like plasminogen activator ( uPA ) than normal cultures but also had higher levels of Fn degradation fragments . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Protease Nexin 1 ( PN 1 ) also known as Glia Derived Nexin ( GDN ) inhibits the activity of several serine proteases including thrombin , tissue ( tPA ) and urokinase ( uPA ) type plasminogen activators . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of the urokinase plasminogen activator ( uPA ) to a specific cell surface receptor ( uPAR ) plays a crucial role in proteolysis during tissue remodelling and cancer invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrin is the dissolving stationary phase and plasminogen , tissue plasminogen activator ( tPA ) , urokinase ( uPA ) , and plasmin are the soluble mobile species . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
ELISA procedures are described for the quantitative analysis of the urokinase type plasminogen activator ( uPA ) and of the tissue type PA ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present study was undertaken to assess the role of membrane associated cathepsin B as an activator of receptor bound single chain urokinase type plasminogen activator ( pro uPA ) and to determine the importance of receptor bound uPA activity in the destruction of extracellular matrix by tumor cells with subsequent invasion through basement membranes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When T47D and MCF 7 human differentiated breast carcinoma cells were treated with the E cadherin antibody DECMA ( decompacting monoclonal antibody ) the cells dissociated from each other and lost their epithelioid morphology , paralleled with a rise in the secretion of urokinase type plasminogen activator ( uPA ) into the extracellular milieu as determined by zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , uPA receptors , and cathepsin B were quantitated by using an immunological method , enzyme linked immunosorbent assay , and amidolytic activity assays in 15 malignant and 10 benign epithelial ovarian tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present report , we describe that affinity purified gp 330 from rabbit renal cortex binds plasminogen activator inhibitor type 1 ( PAI 1 ) complexed with urokinase type plasminogen activator ( uPA ) . alpha 2M methylamine , which binds with high affinity to alpha 2MR / LRP , did not bind to gp 330 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is an extracellular protease and expressed in various cells that exhibit dynamic changes in cell morphology , suggesting a link between cytoskeletal reorganization ( CSR ) and uPA expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunostaining of two invasion associated proteolytic enzymes , cathepsin D ( CD ) and urokinase type plasminogen activator ( uPA ) , was assessed in cryostat sections of 86 stage heterogeneous breast carcinomas using monoclonal antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunotrap assays that can measure the activities of urokinase type plasminogen activator ( uPA ) and its inhibitors ( PAIs ) were characterized . 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The main plasminogen activator ( PA ) form contained in EUs from control mice was defined as murine urokinase type PA ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recent observations have indicated that TNF alpha and lymphotoxin ( TNF beta ) can promote the expression , synthesis and secretion of urokinase plasminogen activator ( uPA ) in human endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There was no correlation between d dimers in the plasma and increased expression of urokinase plasminogen activator ( UPA ) in the tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
ACCS cells also produce a significant amount of proteolytic enzymes , including urokinase type plasminogen activator ( uPA ) , and M ( r ) 72 , 000 and 92 , 000 gelatinases . ^^^ However , ECM elaborated by ACCS cells themselves is resistant to degradation by either the tumor cells or purified uPA in the presence of plasminogen , whereas the degradation rates of ACCS ECM and mesenchymal ECM by plasmin are comparable . ^^^ Treatment of ECM with glycine ( pH 2 . 7 ) , which removes plasminogen activator inhibitor from the matrix , results in an increase in the rate of ACCS ECM degradation by uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using a variety of peptide analogues of oxytocin ( OT ) and Arg 8 vasopressin ( AVP ) , OT mediated induction of urokinase type plasminogen activator ( uPA ) was examined in LLC PK 1 renal epithelial cells , which possess distinct high affinity receptors of both the OT and vasopressin renal ( V 2 ) types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator ( pro uPA ) is bound to a specific surface receptor on ovarian cancer HOC 1 cells that is incompletely saturated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to the differing effects of TGF beta on cell growth , we found that all these cell types showed strong induction of most of the mRNA and protein species examined , including fibronectin , collagen 4 , laminin , type 4 collagenase , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Addition of 1 10 nM urokinase type plasminogen activator ( uPA ) induced adherence in the presence of transforming growth factor type beta 1 , 1 , 25 ( OH ) 2 vitamin D 3 , granulocyte macrophage colony stimulating factor , or tumor necrosis factor alpha . uPA dependent adhesiveness was reversible after 24 h of stimulation with cytokines and uPA as adherence was prevented by the subsequent addition of anti uPA antibodies . ^^^ This difference was largely due to cell surface turnover of active uPA complexed with plasminogen activator inhibitor ( PAI ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) mutants which , at selected amino acid positions , mimic urokinase type plasminogen activator ( uPA ) were expressed in Chinese hamster ovary cells and examined for their catalytic properties . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One of cAMP regulatory sites in the porcine urokinase type plasminogen activator ( uPA ) gene resides 3 . 4 kb upstream of the transcription initiation site and is composed of three protein binding domains , FPA , FPB and FPC . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The prognostic value of urokinase type plasminogen activator ( uPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) levels in cytosolic extracts of ductal breast carcinomas was studied , retrospectively , in 118 premenopausal and 72 postmenopausal high risk patients entered into the protocol of Danish Breast Cancer Cooperative Group trials for adjuvant treatment of breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Complexes between the urokinase type plasminogen activator ( uPA ) and its type 2 inhibitor ( PAI 2 ) are bound by a cell surface receptor for uPA and rapidly cleaved into two fragments of 70 and 22 kDa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolysis triggered by receptor bound urokinase type plasminogen activator ( uPA ) involves a cascade of species specific molecular interactions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Four human glioblastoma cell lines ( U 251 , UWR 1 , UWR 2 , and UWR 3 ) were tested for the expression of the cell surface receptor for urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The glucocorticoid dexamethasone was observed to decrease urokinase plasminogen activator ( uPA ) RNA levels from within 1 h of treatment of MAT 13762 mammary adenocarcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator ( uPA ) , which is elevated in solid tumors , appears to play a key role in these processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , the physiologic inhibitor of both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , is a major biosynthetic product of endothelial cells in vitro ; endothelial cells in vivo , in contrast , do not appear to produce significant amounts of PAI 1 as made evident by in situ hybridization studies in normal mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have observed that a murine IgG 1 monoclonal antibody directed against human urokinase type plasminogen activator ( uPA ) greatly potentiates pro uPA mediated plasminogen activation . ^^^ It appears therefore that the potentiatory effect involves a novel mechanism with the antibody acting to provide a specific template for the assembly of a ternary complex involving pro uPA / uPA and plasminogen , enabling them to interact in a catalytically favorable manner . ^^^ This interpretation was confirmed by studying the kinetics of plasminogen activation by the complex between active , two chain uPA and the antibody , which resulted in an overall 50 fold increase in reaction efficiency ( kcat / Km ) , primarily due to a reduction in Km from 20 to 0 . 1 microM . ^^^ The effects observed here , mediated by ternary complex formation , simulate the effects we have previously observed on assembly of the uPA receptor mediated cellular plasminogen activation system and may therefore represent a mechanistic model for both its activity and initiation . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Further analysis showed that asbestos exposure increased HBE cell associated urokinase type plasminogen activator ( uPA ) activity in a time dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although interaction of tissue plasminogen activator ( tPA ) with fibrin is well established , the prevailing view is that urokinase type plasminogen activator ( uPA ) does not bind to fibrin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using polyclonal antibodies and a fluorescent technique , the immunohistochemical distribution of plasmin / plasminogen , fibrinogen and the plasminogen activators , urokinase ( uPA ) and tissue plasminogen activator ( tPA ) , were studied in lesional and non lesional skin from nine BP patients , one with linear IgA disease ( LAD ) and one with pemphigoid gestationis ( PG ) . ^^^ In normal skin , fibrinogen , tPA and uPA were absent from the epidermis and plasminogen was confined to the basal layer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study we report that exogenous TGF alpha increases urokinase type plasminogen activator ( uPA ) in cultured human keratinocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A search for inhibitors of urokinase type plasminogen activator ( uPA ) in the male and female murine genital tracts revealed high levels of a uPA ligand in the seminal vesicle . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is an important mediator of cellular invasiveness . ^^^ Specifically , cell surface receptor bound uPA activates plasminogen to the potent general protease plasmin , which then degrades extracellular matrix or basement membrane either directly or via proteolytic activation of latent collagenases . ^^^ Two compounds in this class , B 428 and B 623 , inhibit human uPA in plasminogen linked assays with median inhibition concentration ( IC 50 ) values of 0 . 32 and 0 . 07 microM , respectively . ^^^ Importantly , both compounds show > 300 fold selectivity for uPA relative to tissue type plasminogen activator and > 1000 fold selectivity relative to plasmin . ^^^ Moreover , degradation of [ 3H ] fibronectin by HT 1080 cells via cell surface uPA mediated , plasminogen dependent mechanisms is inhibited by B 428 and B 623 , with IC 50 values of 1 . 5 and 0 . 39 microM , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mononuclear phagocytes regulate net PA activity by modulating the expression of urokinase type PA ( uPA ) and a specific plasminogen activator inhibitor , PAI 2 . ^^^ We further conclude that cytokine specific regulation of plasminogen activity is achieved partly by varying the proportionate expression of uPA and PAI 2 . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The biochemical , regulatory , and physiological aspects of plasmin formation by the urokinase type plasminogen activator ( uPA ) is discussed and its involvement in cancer malignancy analyzed . ^^^ The ability of cells to produce plasmin on their surface depends on the presence of uPA and plasminogen receptors , and is the basis of the regulation of the plasminogen activating system in vivo . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have determined whether macrophage derived foam cells , a prominent component of the atherosclerotic lesion , express more urokinase type plasminogen activator ( uPA ) and whether their ability to generate plasmin stimulates the release of matrix bound growth factors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A series of chimeric urokinase type plasminogen activator ( uPA ) genes , which contain combinations of kringle domains of human plasminogen ( HPg ) in place of the uPA kringle ( KuPA ) , has been constructed and expressed . ^^^ Small differences are also observed in the abilities of these tcr variants to interact with the fast acting plasma inhibitor of uPA , viz . , plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Neither wtr uPA nor any of its chimeric r variants interacted macroscopically with a fibrin clot under conditions that allowed binding of 74 % of single chain r tissue type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Evaluation of expression of tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , metalloproteinases and TIMP 1 was performed by means of enzyme immunoassay , zymogram , or immunoblot . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of IGFs / GFBPs , urokinase type plasminogen activator ( uPA ) , as well as of TGF beta 1 and glucocorticoids is discussed in this review . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and tissue plasminogen activator ( tPA ) were assayed in biopsies from the base , edge and adjacent skin of ischaemic and venous ulcers using a functional bioimmunoassay and a standard immunoassay . ^^^ The predominant plasminogen activator in chronic leg ulcers is uPA ; this activator may play an important role in wound healing . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Flow cytometry evaluation of urokinase type plasminogen activator receptor ( UPA R ) in acute myeloid leukemia cells . ^^^ The aim of this study was to investigate by flow cytometry the expression of the UPA R ( Urokinase type plasminogen activator receptor CD 87 ) on the blastic population of AML and ALL patients in order to evaluate whether the presence of this molecule could be associated with peculiar clinical and biologic features of leukemic cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The GPI anchored urokinase plasminogen activator receptor ( uPAR ) does not internalize free urokinase ( uPA ) . ^^^ On the contrary , uPAR bound complexes of uPA with its serpin inhibitors PAI 1 ( plasminogen activator inhibitor type 1 ) or PN 1 ( protease nexin 1 ) are readily internalized in several cell types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we further investigated changes in expression of both tPA and urokinase type plasminogen activator ( uPA ) activity during various developmental stages of rat corpus luteum ( CL ) of pregnancy and their possible physiological roles in luteolysis . ^^^ Rat CL or dispersed luteal cells in vitro are capable of producing both tPA and uPA , and a plasminogen activator inhibitor type 1 , in a stage dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have used a group of human microplasminogens ( mPlg ) , modified by residue substitutions , insertions , deletions , and chain breaks ( 1 ) to study the determinants of productive interactions with two plasminogen activators , urokinase ( uPA ) , and streptokinase ( SK ) ; ( 2 ) to explore the basis of species specificity in the zymogen SK complex activity ; and ( 3 ) to compare active SK complex formation in mPlg and microplasmin ( mPlm ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here we show that the P 1 ' residue of the plasminogen activator inhibitor type 1 mutant ( P 1 ' Cys ) became more accessible to radiolabeling in complex with urokinase type plasminogen activator ( uPA ) compared with its complex with catalytically inactive anhydro uPA , indicating that complex formation with cognate protease leads to a conformational change whereby the P 1 ' residue becomes more accessible . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Methods used to assess the expression and levels of urokinase type plasminogen activator ( uPA ) or plasminogen activator inhibitor 1 ( PAI 1 ) have also been developed , and correlate with angiogenic activity and prognosis of patients with breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , and streptokinase were evaluated for their ability to reduce postsurgical adhesion formation in a rat uterine horn devascularization and serosal injury model in a blinded , randomized study . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Three major components of the plasminogen activators ( PA ) / plasmin system are synthesized physiologically in glomeruli , and can be involved in glomerular proteolysis and extracellular matrix metabolism : tissue type PA ( tPA ) , urokinase ( uPA ) and PA inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the effect of FGF 2 ( basic FGF ) on the urokinase type plasminogen activator ( uPA ) gene in NIH 3T3 fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cultured baboon ECs transduced with human cDNAs for wild type tissue plasminogen activator ( TPA ) or for glycosylphosphatidylinositol anchored urokinase type plasminogen activator ( a UPA ) were seeded onto collagen coated segments of vascular graft ( collagen segments ) and exposed overnight to flow using an in vitro perfusion circuit . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , we included studies on urokinase type plasminogen activator ( uPA ) , because Northern blot analysis showed a strong coincidence of uPA expression with oval cell proliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Therefore , we evaluated whether thrombin affected the expression of endometrial stromal cell urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators and their primary inhibitor , type 1 plasminogen activator inhibitor ( PAI 1 ) , and whether ovarian steroids modulated putative thrombin effects . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is present in prostatic secretion , and an increased uPA activity has been noted in human prostatic cell lines with metastatic behavior . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( CD 87 ) : a new target in tumor invasion and metastasis . ^^^ Four different classes of proteases are known to be correlated with the malignant phenotype : 1 ) Matrix metalloproteases ; including collagenases , gelatinases and stromelysins . 2 ) Cysteine proteases ; including cathepsins B and L . 3 ) Aspartyl protease cathepsin D . 4 ) Serine proteases ; including plasmin and tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cleavage of labeled [ Glu ] Pg 1 by urokinase type plasminogen activator ( uPA ) was accompanied by a fluorescence enhancement ( delta Fmax / Fo ) of 2 . 0 , and formation of 1 % plasmin ( Pm ) activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have conjugated the ribosome inactivating protein ( RIP ) saporin to human urokinase type plasminogen activator ( uPA ) , and tested the uPA saporin conjugate for cytotoxicity to uPA receptor expressing cells . ^^^ Unlike unconjugated uPA , saporin conjugated uPA did not require any interaction with plasminogen activator inhibitors to be internalized . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The enhanced PA activities were completely inhibited by anti urokinase type plasminogen activator ( uPA ) IgG . ^^^ These findings suggest that a population of activated lymphocytes produces a plasminogen activator modulator that induces uPA on the surface of malignant tumor cells . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine proteinase involved in degradation of the extracellular matrix during cancer invasion . uPA is up regulated in breast cancer , and high levels of uPA in tumor extracts are strongly associated with poor prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Among the genes regulated by middle T , the urokinase type plasminogen activator ( uPA ) gene seems to be of primary importance for the development of the transformed phenotype . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There is ample evidence that the protease urokinase plasminogen activator ( uPA ) plays a role in invasion and spread of tumours . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Enzyme immunoassays ( EIAs ) showed that urokinase and tissue plasminogen activators ( uPA , tPA ) and PA inhibitors ( PAI 1 , PAI 2 ) were present in ascites from both patient groups and that tPA was the predominant PA . uPA , tPA , and PAI 1 , were detected in plasma from patients and controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These findings suggest that retinoic acid increases the amount of receptor bound uPA via de novo synthesis , and that it plays an important role in modulating cell associated plasminogen activation . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The facilitating effect of fibrin on tube formation by BCEs was inhibited by either anti basic fibroblast growth factor ( bFGF ) IgG ( 25 micrograms / ml ) or anti urokinase type plasminogen activator ( uPA ) IgG ( 10 micrograms / ml ) added to the gel and culture medium , but not by anti tissue type plasminogen activator ( uPA ) IgG ( 10 micrograms / ml ) added to the gel and culture medium , but not by anti tissue type plasminogen activator ( 10 micrograms / ml ) or non immune IgG . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) converts plasminogen to plasmin which degrades various extracellular matrix components . uPA is focused to the cell surface via binding to a specific receptor ( uPAR , also termed CD 87 ) . uPAR bound uPA mediates pericellular proteolysis in a variety of biological processes , e . g . cell migration , tissue remodeling and tumor invasion . ^^^ The amount of receptor bound uPA is then determined indirectly via addition of plasminogen , which is activated to plasmin , followed by cleavage of a plasmin specific chromogenic substrate . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Within 5 days , smooth muscle cells ( SMCs ) on the luminal surface expressed the mRNA for tissue type plasminogen activator ( TPA ) , urokinase type plasminogen ( UPA ) , the receptor for UPA ( UPAR ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Keratinocytes synthesize and secrete urokinase type plasminogen activator ( uPA ) which is bound in an autocrine manner to a specific receptor ( uPA R ) at the keratinocyte surface . ^^^ Plasminogen that is also bound to specific membrane binding sites is readily activated by uPA R bound uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator ( PA ) proteolytic cascade comprises two enzymes known as urokinase PA ( uPA ) and tissue PA ( tPA ) , both of which activate plasminogen to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cells of early passages ( passage no . 22 ) only expressed urokinase plasminogen activator ( uPA ) but not tPA specific mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is synthesized as single chain protein ( scuPA ) with little intrinsic activity . scuPA is activated when it is converted to two chain urokinase ( tcuPA ) by plasmin or when it binds as a single chain molecule to its cellular receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Physiological concentrations of urokinase plasminogen activator ( uPA ) stimulated a chemotactic response in human monocytic THP 1 through binding to the urokinase receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
While TPA treatment evoked a temporary increased expression of urokinase type PA ( uPA ) , the production of both types of human plasminogen activator inhibitors ( PAI ) was induced and sustained over 12 h by TPA treatment shifting the protease protease inhibitors balance in favor of the inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It was found that urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were expressed diffusely in most thyroid carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We assessed the invasive capacities and expression of urokinase type plasminogen activator ( uPA ) in the estrogen receptor ( ER ) negative MDA MB 231 cell line , and the same cell transfected with an ER expression vector ( S 30 cells ) in response to 17 beta estradiol ( E 2 ; 1 nM ) and epidermal growth factor ( EGF ; 1 ng / ml and 10 ng / ml ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TGF beta 1 increases fibronectin expression and urokinase plasminogen activator ( uPA ) activity and plasminogen activator inhibitor 1 ( PAI 1 ) levels in 3D cultures . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cellular distribution of mRNAS for urokinase type plasminogen activator ( uPA ) , its specific receptor ( uPAR ) , and its inhibitors ( PAI 1 and 2 ) in mouse skin was analyzed by in situ hybridization after topical application of the tumor promoter phorbol 12 myristate 13 acetate . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrin gel degradation occurred through plasmin mediated fibrinolysis , which was initiated by fibroblasts exhibited high uPA but low plasminogen activator inhibitor 1 ( PAI 1 ) activities , and transforming growth factor beta 1 prevented fibrinolysis of normal fibroblasts by upregulating PAI 1 while downregulating uPA activities . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , single deficient mice lacking tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , or PA inhibitor type 1 ( PAI 1 ) gene function were recently found to have normal reproduction , although mice with a combined deficiency of tPA and uPA were significantly less fertile . ^^^ This result suggests that plasminogen activation plays a role in ovulatory response , although neither tPA nor uPA individually or in combination is obligatory for ovulation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , LY 290181 inhibited phorbol ester stimulated activation of the porcine urokinase plasminogen activator ( uPA ) promoter ( 4600 / +398 ) linked to the chloramphenicol acetyltransferase ( CAT ) reporter gene ( p4660CAT ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A strong correlation has previously been demonstrated between the activation of the urokinase plasminogen activator ( uPA ) proteolysis network and the acquisition of the invasive metastatic phenotype , and we show here that HGF / SF Met signalling significantly increases the protein levels of both uPA and its cellular receptor in SK LMS 1 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of urokinase plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and interleukin 1beta were all found to be significantly different in interfaces and in pseudocapsules , compared to controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prognostic relevance of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitors PAI 1 and PAI 2 in gastric cancer . ^^^ Expression of urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) was evaluated in 125 surgically resected gastric cancers by immunohistochemical analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the role of plasminogen activators ( tPA uPA ) and inhibitor ( PAI 1 ) in the pathogenesis of preeclampsia . ^^^ CONCLUSIONS : The elevated PAI 1 level is associated with the preeclampsia and the imbalance between the plasminogen activators ( tPA , uPA ) and the inhibitor ( PAI 1 ) might be involved in the pathogenesis of preeclampsia . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cysteine proteases ( cathepsin B [ CATB ] and cathepsin L [ CATL ] ) , the serine protease urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) are thought to play an important part in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
E cadherin ( ECD ) is known to be an invasion suppressor gene , and urokinase type plasminogen activator ( uPA ) plays a central role in infiltration of solid cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation was assessed by measuring the plasmin activity and plasminogen activator inhibitor 1 ( PAI 1 ) levels in cell conditioned media and the cell associated urokinase type plasminogen activator ( uPA ) levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and its receptor are key components of a cell surface proteolytic cascade used by tumor cells and capillary endothelial cells for basement membrane invasion , a process required for metastasis and angiogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrin zymography revealed the presence of high molecular weight urokinase type plasminogen activator ( uPA ) , low molecular weight uPA , and uPA / inhibitor complexes within endothelial cultures and cocultures . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We further investigated the effects of IGFs on the secretion of a key enzyme in the metastatic cascade , the urokinase type plasminogen activator ( uPA ) in M 3 and MM 3 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : A strong positive correlation exists between the breast cancer tissue content of either urokinase plasminogen activator ( uPA ) or plasminogen activator , inhibitor type 1 ( PAI 1 ) , quantified in the tissue extracts by immunoassays , and the survival of patients with breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is expressed on the cell surface of B 16 murine melanoma cells and is thought to contribute to the pericellular proteolysis necessary for tumor cell migration . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , a rapid inhibitor of both uPA and tissue type plasminogen activator ( tPA ) is the major physiologic regulator of plasminogen activator activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We measured levels of MMPs , urokinase type plasminogen activator ( uPA ) , and TIMPs by zymography , reverse zymography , and Western blots . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The availability of gene targeted mice deficient in the urokinase type plasminogen activator ( uPA ) , urokinase receptor ( uPAR ) , tissue type plasminogen activator ( tPA ) , and plasminogen permits a critical , genetic based analysis of the physiological and pathological roles of the two mammalian plasminogen activators . ^^^ These results indicate that plasminogen activation focused at the cell surface by uPAR is important in fibrin surveillance in the liver , but that uPA supplies sufficient fibrinolytic potential to clear fibrin deposits from most tissues and support wound healing without the benefit of either uPAR or tPA . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both in cell culture and in vivo , keratinocytes that are migrating in response to a wound express enhanced levels of both urokinase type plasminogen activator ( uPA ) and the uPA cell surface receptor ( uPA R ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Borrelia burgdorferi binds human urokinase plasminogen activator ( uPA ) , which cleaves plasminogen ( Pgn ) to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation catalyzed by the urokinase type plasminogen activator ( uPA ) constitutes a reciprocal zymogen activation system , as plasmin can efficiently activate pro uPA , the single chain zymogenic form of the protease . ^^^ We have previously shown that the overall efficiency of this plasminogen activation system is greatly enhanced by its assembly on the cell surface , involving binding of pro uPA to its cellular binding site uPAR , and the concurrent cellular binding of plasminogen . ^^^ In contrast to the increased efficiencies of plasminogen activation and pro uPA activation observed with cell surface uPAR , soluble uPAR had an inhibitory effect on both of these individual reactions . ^^^ Consistent with the observations on the isolated reactions , the overall activity of the pro uPA mediated plasminogen activation system was significantly inhibited . ^^^ These observations confirm the previous interpretation of the observations made with cell surface uPAR that the mechanism of the enhanced plasmin generation is due to the catalytically favorable interaction of uPAR bound uPA / pro uPA with cell bound plasminogen / plasmin , rather than direct effects on the properties of uPA or pro uPA on binding to uPAR . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to further study the role of plasmin in cancer progression , we have characterized urokinase type plasminogen activator ( uPA ) mediated plasmin formation in three human breast cancer cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator ( uPA ) by malignant cells correlates with an aggressive phenotype , including increased invasiveness , tumor associated angiogenesis , and metastases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , a proteinase which activates plasminogen by cleaving at CPGR ( arrow downward ) 5 , was shown to cleave the V 3 loop in recombinant gp 120 of human immunodeficiency virus type 1 ( HIV 1 ) IIIB and MN strains , as well as a synthetic , cyclized peptide representing the clade B consensus sequence of V 3 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of urokinase type plasminogen activator ( uPA ) to a specific receptor ( uPAR ) on human lung fibroblasts enables it to regulate cellular proteolysis and remodeling of the extracellular matrix . ^^^ Phorbol myristate acetate , lipopolysaccharide , transforming growth factor beta ( TGF beta ) , and tumor necrosis factor alpha ( TNF alpha ) increased uPA binding and plasminogen activation at the cell surface , with a greater maximal effect on fibrotic than on normal fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The lysosomal protease Cathepsin D and the serine protease urokinase plasminogen activator ( uPA ) are suspected to indicate poor prognosis in primary breast carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In breast cancer , receptor bound urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 seem to play an important role in the dissolution of the surrounding tissue and the formation of tumour stroma . ^^^ In particular , low tissue plasminogen activator ( tPA ) levels , as antigen or as activity , high uPA : tPA antigen ratio in corresponding normal mucosa , high levels of uPA related antigen and activity and of PAI 2 antigen in neoplastic tissue , and high uPA ( neoplastic mucosa ) : tPA ( normal mucosa ) ratio , were all parameters associated with a poor overall survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vitro anti proliferative and anti invasive role of aminoterminal fragment of urokinase type plasminogen activator on 8701 BC breast cancer cells . 8701 BC cells , derived from a primary carcinoma of the breast , constitutively express mRNA for urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of urokinase type plasminogen activator ( uPA ) to its glycosylphosphatidylinositol anchored receptor ( uPAR ) initiates signal transduction , adhesion , and migration in certain cell types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate the regulation of promoters containing classical phorbol ester response sequences ( PEA 3 / 12 O tetradecanoylphorbol 13 acetate response element motifs ) by protein kinase C ( PKC ) isozymes , co transfections were performed in human dermal fibroblasts with a plasmid containing either the human collagenase promoter or the porcine urokinase plasminogen activator ( uPA ) promoter linked to the chloramphenicol acetyltransferase gene and a plasmid expressing an individual PKC isozyme . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen and plasmin system , which is mainly regulated by urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) and its inhibitor ( PAI 1 ) , is generally believed to play a role in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is an inducible serine protease , secreted by a variety of cell types , that functions in fibrinolysis and has been implicated also in events such as cell migration and tissue remodeling and repair . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Earlier , we proposed that matrix metalloproteinases ( MMPs ) and urokinase type plasminogen activator ( uPA ) are important in secondary brain injury . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator ( PA ) , particularly the urokinase type PA ( uPA ) , has been implicated in tissue remodeling . ^^^ Northern blot analyses of RNA from the cultured cells demonstrated that the steady state levels of mRNA for uPA , but not for tPA and plasminogen activator inhibitor 1 , were decreased by indomethacin ; this decrease was reversed by prostaglandin E 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Under certain pathophysiological conditions epidermal keratinocytes produce urokinase type plasminogen activator ( uPA ) or tissue type PA ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The association of urokinase type plasminogen activator ( uPA ) with its receptor ( uPAR ) influences various biologic functions , including cell migration , angiogenesis , differentiation , and wound healing . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The properties studied include specificity for urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators , binding to conA and heparin , inhibition of thrombin , plasmin and trypsin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human cell lines of myelo / monocytic origin express the cellular receptor for urokinase type plasminogen activator ( uPA R ) . ^^^ The receptor localizes urokinase type plasminogen activator ( uPA ) to the surface of the cell , where it can convert plasminogen to the active serine proteinase plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of primary cutaneous melanocytic neoplasms in urokinase type plasminogen activator ( uPA ) deficient and wild type mice : cellular blue nevi invade but do not progress to malignant melanoma in uPA deficient animals . ^^^ Evidence suggests that the plasminogen activators ( PAs ) , in particular urokinase type PA ( uPA ) , play a pivotal role in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We examined the effects of inflammatory cytokines , such as interleukin 1 alpha ( IL 1 alpha ) , interleukin 1 beta ( IL 1 beta ) , interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , transforming growth factor beta ( TGF beta ) and lipopolysaccharide ( LPS ) , on the urokinase type plasminogen activator ( uPA ) gene expression in RC K 8 human pre B lymphoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS AND RESULTS : When human cerebral microvascular endothelial cells ( HCMEC ) , pial arterial endothelial cells , and middle meningeal arterial endothelial cells were exposed to 10 to 1000 ng / mL recombinant tissue type plasminogen activator ( RTPA ) , urokinase type plasminogen activator ( UPA ) , or streptokinase / plasminogen ( 37 U streptokinase plus 2 mumol / L plasminogen ) for 24 hours , they exhibited concentration dependent decreases in elaboration of PAI 1 of 65 + / 3 % , 48 + / 3 % , and 59 + / 8 % . ^^^ UPA and streptokinase / plasminogen elicited decreases of 33 + / 8 % and 35 + / 4 % , respectively , that were specific with respect to the protease agonists as to total protein synthesis and cell type ; ie , neither human umbilical vein endothelial cells nor cerebral pericytes exhibited inhibition of PAI 1 elaboration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In situ hybridization was performed to localize collagenase , stromelysin 1 , stromelysin 2 , matrilysin , urokinase plasminogen activator ( uPA ) and TIMP 1 mRNAs in 14 chronic venous ulcers and 10 normally healing wounds , representing different time points after wounding . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activators , tissue type and urokinase type ( tPA and uPA , respectively ) , have been identified in various malignancies and have been implicated in both local growth and metastatic spread . ^^^ To characterize plasminogen activator expression more fully in human basal cell carcinoma , the localization of uPA and tPA mRNAs was evaluated by in situ hybridization . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : A marked increase in the expression of tissue factor ( TF ) and type 1 plasminogen activator inhibitor ( PAI 1 ) and an inhibition of tissue type and urokinase type plasminogen activators ( tPA and uPA , respectively ) , matrix metalloproteinases ( MMP ) , and endothelin 1 ( ET 1 ) expression accompany progestin induced decidualization of estrogen primed endometrial stromal cells both in vivo and in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RSVCEFs also exhibit high levels of urokinase type plasminogen activator ( uPA ) , which is found in active form in their conditioned medium in complete absence of plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a multifunctional protein involved in both extracellular proteolysis and signal transduction . uPA usually mediates its actions while attached to a membrane bound receptor , termed uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator ( PA ) / plasmin system has been extensively investigated in these processes , and descriptive studies have demonstrated that urokinase type PA ( uPA ) , uPA receptor ( uPAR ) and PA inhibitor 1 ( PAI 1 ) are expressed by endothelial cells during angiogenesis in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The local and systemic invasiveness of soft tissue sarcomas may depend upon an interaction between the primary tumour and the extracellular matrix in which the proteolytic enzyme , urokinase plasminogen activator ( uPA ) , may have an important role . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The sites of synthesis of urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and alpha 2 macroglobulin receptor / low density lipoprotein receptor related protein ( alpha 2MR / LRP ) transcripts were determined by in situ hybridization . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is an important regulator of plasminogen activation , which inhibits both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The extracellular proteolytic pathway mediated by the urokinase plasminogen activator ( uPA ) is a cascade system , initiated by activation of the zymogen , pro uPA . ^^^ In contrast , the progress of the activation reactions in a soluble system with pro uPA and plasminogen was found to be attenuated by suPAR . ^^^ This delay of the activation system was shown to include a partial inhibition of the plasmin mediated activation of pro uPA as well as of the uPA mediated activation of plasminogen . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor cells produce urokinase type plasminogen activator ( uPA ) in an enzymatically inactive proenzyme form ( pro uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To determine whether platelet activating factor ( PAF ) , a lipid mediator that is accumulated in the cornea after alkali burn , induces the gene expression of urokinase type plasminogen activator ( uPA ) in the corneal epithelium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation by urokinase type plasminogen activator ( uPA ) is implicated in tumor invasion and metastasis by the breakdown of extracellular matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) is secreted into the urine at high concentrations and both the uPA protein and mRNA are present in human renal tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumour necrosis factor alpha ( TNF alpha ) and interleukin 1 beta ( IL 1 beta ) increased in several , but not all , cell lines the production of urokinase type plasminogen activator ( uPA ) , tissue type PA ( tPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) as analysed by zymography , enzyme immunoassays and Northern analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Strong correlations have been reported between expression of urokinase type plasminogen activator ( uPA ) and poor prognosis in several human carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of the urokinase type plasminogen activator receptor ( uPAR ) promotes cell adhesion through its interaction with vitronectin ( VN ) in the extracellular matrix , and facilitates cell migration and invasion by localizing uPA to the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This review focuses on the main components of the fibrinolytic system tissue plasminogen activator ( tPA ) , urokinase ( uPA ) and plasminogen activator inhibitor ( PAI 1 ) and on thrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We examined the activity of the serine protease urokinase type plasminogen activator ( uPA ) present in the euglobulin fraction of plasma from 17 demented patients with probable Alzheimer ' s disease ( AD ) , 12 patients with vascular dementia ( VD ) and 10 healthy controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The prognostic value of tumour associated proteolysis , especially the urokinase plasminogen activator ( uPA ) system , has been proposed in gastric cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prevention of prostate cancer metastasis in vivo by a novel synthetic inhibitor of urokinase type plasminogen activator ( uPA ) . ^^^ Urokinase type plasminogen activator ( uPA ) is a serine protease associated with tissue remodeling , cellular invasiveness , matrix degradation and tumor growth . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It has been demonstrated that prostate cancer cells over express urokinase type plasminogen activator ( uPA ) , which plays an important role in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : It has been proposed that the serine protease urokinase type plasminogen activator ( UPA ) and its inhibitor type 1 ( PAI 1 ) may play an important part in cancer spread and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The proteolytic enzyme urokinase type plasminogen activator ( uPA ) plays an important role in degrading extracellular matrix . ^^^ In this study we investigated the correlation of the Plasminogen Activator Inhibitor 1 ( PAI 1 ) , which is the specific inhibitor of uPA and which seems to be important for tumour formation , with prognosis in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One of the mechanisms by which endothelial cells ( ECs ) regulate fibrinolysis is through the regulated assembly of proteins such as plasminogen , tissue plasminogen activator ( tPA ) and urokinase ( uPA ) on their membrane surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have compared by RNA in situ hybridisation on serial cryo sections the distribution of cathepsin D ( cathD ) , stromelysin 3 ( strom 3 ) , and urokinase plasminogen activator ( UPA ) gene expression in different tissues of human benign and malignant mammary tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Rat endometrial stromal cells undergoing decidualization in vitro secrete urokinase type plasminogen activator ( uPA ) , and this secretion is regulated by prostaglandin E 2 . ^^^ The present study was undertaken to determine whether uPA and plasminogen activator inhibitor 1 ( PAI 1 ) mRNAs are expressed in vivo in the decidua of pregnant rats and in the deciduoma of `` pseudopregnant ' ' rats . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Basic fibroblast growth factor ( FGF 2 ) induces cell proliferation and urokinase type plasminogen activator ( uPA ) production in fetal bovine aortic endothelial GM 7373 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunoassays ( ELISA ) of urokinase type plasminogen activator ( uPA ) : report of an EORTC / BIOMED 1 workshop . ^^^ The urokinase type plasminogen activator ( uPA ) is considered to play a key role in the process of invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Median levels of urokinase plasminogen activator ( uPA ) and the uPA receptor were higher in carcinoma than in matched normal mucosa ( squamous cell carcinoma : uPA 4 . 05 versus 0 . 66 ng antigen per mg protein , uPA receptor 1 . 95 versus 0 . 50 ng / mg , n = 10 , P < 0 . 05 ; adenocarcinoma : uPA 2 . 16 versus 0 . 61 ng / mg , uPA receptor 2 . 01 versus 0 . 49 ng / mg , n = 8 , P < 0 . 05 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) , when bound to its cell surface receptor ( uPAR ) , is an active cell surface protease . uPAR expression has been associated with cell activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Keratinocytes synthesize urokinase type plasminogen activator ( uPA ) and a specific cell surface receptor for uPA ( uPA R , CD 87 ) . ^^^ Plasminogen is present in plasma and interstitial fluids from where it is bound to cell surfaces via plasmin ( ogen ) binding sites . uPA binds to the uPA R in an autocrine manner and activates cell bound plasminogen : a mechanism , which provides plasmin for pericellular proteolysis . ^^^ Cell bound uPA is regulated by plasminogen activator inhibitor type 1 ( PAI 1 ) or type 2 ( PAI 2 ) . ^^^ Although circumstantial evidence suggested plasminogen activation in lesional epidermis of bullous pemphigoid , immunohistological data on the type of plasminogen activators , on the uPA receptor or the type of plasminogen activator inhibitors in the lesions of bullous pemphigoid are lacking so far . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of urokinase type plasminogen activator ( uPA ) was investigated in squamous cell carcinoma of the human larynx . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Due to their role in invasiveness and angiogenesis , we also observed the expression of urokinase plasminogen activator ( uPA ) , uPA receptor , and plasminogen activator inhibitor type 1 by TTB cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) initiates an extracellular proteolytic cascade with which invasive cells eliminate barriers to movement . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation of plasminogen is initiated by urokinase plasminogen activator ( uPA ) . ^^^ The binding of uPA to the uPA cell surface receptor ( uPA R ) accelerates plasmin generation from plasminogen and localizes uPA activity to the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : Urokinase type plasminogen activator ( uPA ) , uPA receptor , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , matrix metalloproteinases MMP 8 , MMP 9 and tissue inhibitor of metalloproteinases TIMP 1 were measured by ELISA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using substrate zymograms , reverse zymograms and Western immunoblots , we found that PDL cells constitutively express 72 kDa gelatinase , urokinase type plasminogen activator ( uPA ) and at least three inhibitors whose molecular masses correspond to those of the tissue inhibitors of metalloproteinases ( TIMPs ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Addition of basic fibroblast growth factor ( bFGF ) to a rat mammary gland myoepithelial cell line ( 25 . 5 G4 . 2 . 3 ) resulted in a six to eightfold increase in cellular and secreted urokinase type plasminogen activator ( uPA ) activity after a lag phase of 5 8 h . bFGF had no effect on the uPA activity of mammary epithelial cells . bFGF was active on myoepithelial cells over a narrow concentration range ( 0 . 5 2 ng / ml ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Accordingly , PAI 1 was recovered in a reactive centre cleaved form from incubations with urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) at 0 degree C , but not at 37 degrees C . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since TNF alpha may contribute to thrombotic complications in sepsis patients , we determined markers of thrombin activation , parameters of the fibrinolytic system ( D dimer , tissue plasminogen activator antigen ( tPA ) urinary type plasminogen activator antigen ( uPA ) , plasminogen activator inhibitor antigen ( PAI 1 ) and von Willebrand factor antigen ( vWF ) in 30 patients with sepsis or septic shock . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) generates plasmin , a process inhibited by plasminogen activator inhibitor ( PAI ) 1 and localized to the cell surface by binding of uPA to a specific receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously reported that culture medium conditioned by human SK Hep 1 hepatoma cells or mouse S 180 sarcoma cells induces in vitro angiogenesis and stimulates production of urokinase plasminogen activator ( uPA ) in vascular endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) interacts with its cell surface receptor ( uPAR ) , providing an inducible , localized cell surface proteolytic activity , thereby promoting cellular invasion . ^^^ Moreover , plasminogen activator inhibitor type 2 blocked this migration event but did not affect adhesion , suggesting a direct role for uPA enzyme activity in this process and that migration but not adhesion of these cells is regulated by uPA . uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is produced and secreted by multiple vascular cell types , thus influencing the processes and the extent to which the vasculature is remodelled during the development of the intima or a neointima and during hypertrophy and angiogenesis . 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The activities , antigens and messenger RNA ( mRNA ) levels of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in articular cartilage were measured in patients with no history of joint diseases ( control ) , those with osteoarthritis ( OA ) classified into osteophyte formed site ( OS ) and weight bearing site ( WS ) , and in patients with rheumatoid arthritis ( RA ) . 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Dispase mediated basal detachment of cultured keratinocytes induces urokinase type plasminogen activator ( uPA ) and its receptor ( uPA R , CD 87 ) . ^^^ Keratinocytes synthesize and secrete urokinase type plasminogen activator ( uPA ) , which is bound in an autocrine manner to a specific receptor ( uPA R , CD 87 ) at their surface . ^^^ Plasminogen , which is also bound to membrane binding sites , is readily activated by uPA R bound uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increase of cellular fibrinolysis in human lung cancer cell line by radiation : relationship between urokinase type plasminogen activator ( uPA ) and metastasis and invasion ] . ^^^ It is well known that urokinase type plasminogen activator ( uPA ) activates fibrinolysis of tumor cells and accelerates their metastasis and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Then , we analyzed the morphological evidence of osteoblasts reaction , local invasion around the inoculated cancer cells and expression of the cathepsin D and urokinase type plasminogen activator ( uPA ) around the sites of inoculation using immunocytochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : The plasminogen system , which includes tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and their main inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) , plays a major role in both fibrinolysis and tissue remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of matrix metalloproteinase 1 ( MMP 1 ) , matrix metalloproteinase 8 ( MMP 8 ) , matrix metalloproteinase 9 ( MMP 9 ) , lactoferrin and urokinase plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) and the inhibitors , tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) , plasminogen activator inhibitor 1 ( PAI 1 ) , plasminogen activator inhibitor ( PAI 2 ) , and alpha 2 macroglobulin in the synovial fluids of patients with rheumatoid arthritis was determined before and during chemical synoviorthesis with a sodium salt of the fatty acids from cod liver oil ( Varicocid ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , anticatalytic uPA antibody blocked the plasminogen enhanced NC cell migration showing that NC cell associated uPA activity was required for this effect . ^^^ Finally , decreasing NC uPA activity by treating cells with transforming growth factor Beta , also blocked the plasminogen dependent increase in cell migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To clarify the specific expression and regulation of mRNAs for urokinase ( uPA ) , its receptor ( uPAR ) , and type 1 plasminogen activator inhibitor ( PAI 1 ) , 1 analyzed RNA from four human cancer cell lines by RNA blotting after treatment with cycloheximide , anisomycin , emetine or puromycin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this report , we show that both intact B . burgdorferi organisms and its recombinant outer surface lipoprotein A induce human monocytes to express and secrete urokinase type plasminogen activator in its zymogen form ( pro uPA ) . ^^^ Taken together , the data suggest that B . burgdorferi may induce pro uPA in a monocyte containing inflammatory site and that the spirochetal surface provides an appropriate milieu for subsequent interactions between ( pro ) uPA and plasmin ( ogen ) , which result in spirochete bound plasmin even in the presence of inhibitors for plasminogen activators and plasmin . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The incubation of mesangial cells with TSP increased the secretion of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , while plasminogen activator inhibitor type 1 ( PAI 1 ) decreased . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is an important protease enzyme in carcinogenesis , and is involved in both invasion and metastasis of cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were administered to determine the effect of TGF beta activation on the axial length in normal and FDM eyes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to clarify a role of stromal cells in sex steroidal neovascularization , plasminogen activator inhibitor ( PAI ) 1 [ an inhibitor of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) ] and its messenger ribonucleic acid ( mRNA ) were analysed in fibroblasts derived from uterine endometrium as a model for endometrial stromal cells under the influence of sex steroids . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Consistent with this , the ovulation efficiency of mice lacking the two known physiological plasminogen activators ( PAs ) , tissue type PA ( tPA ) and urokinase type PA ( uPA ) , is reduced by 26 % . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Many lines of evidence support an involvement of urokinase plasminogen activator ( uPA ) and its type 1 inhibitor ( PAI 1 ) in the migration of a variety of cells , including normal keratinocytes and carcinoma lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition of activated protein C ( APC ) , urokinase plasminogen activator ( uPA ) , tissue plasminogen activator ( tPA ) , thrombin , factor Xa ( Xa ) , factor XIa ( XIa ) and plasma kallikrein ( KK ) by PCI was found to be dependent on the size of the polysaccharide . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urinary type plasminogen activator , urokinase ( uPA ) is localized on the cell surface through the binding of a specific receptor , the uPA receptor ( uPAR ) . ^^^ The comparison of uPAR expression between these cell lines and peripheral blood cells and it correlation with plasminogen receptors , tPA receptors and LRP expression offers new insights regarding potential mechanisms for regulation of uPA uPAR mediated pericellular proteolysis . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study demonstrated the profile of the neutral proteinases , 1 ) matrix metalloproteinases ( MMP ) 1 , 2 , 3 , and 9 , and 2 ) serine proteinases , elastase , cathepsin G , urokinase and tissue type plasminogen activators ( uPA and tPA ) as well as their inhibitors , namely , tissue inhibitor of matrix metalloproteinases ( TIMP ) 1 , alpha 1 antitrypsin , alpha 1 antichymotrypsin , plasminogen activator inhibitor ( PAI ) 1 & 2 , around loose hip prostheses to clarify the step in the cascade of biological host response in the loosening of replaced total hip joints . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator system ( PA system ) consists of urokinase type plasminogen activator ( uPA ) , tissue type PA ( tPA ) , as well as the two types of plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . ^^^ The central PA system component plasminogen , which is present in plasma and interstitial fluids , is bound to the keratinocyte surface via plasmin ( ogen ) binding sites , where it can be activated by uPA R bound uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 in benign , borderline , malignant primary and metastatic ovarian tumors . ^^^ Elevated levels of expression of the urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 have shown to be related to poor prognosis in a variety of cancer types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied the temporal expression of interstitial collagenase , stromelysin 1 and 2 , and urokinase plasminogen activator ( uPA ) mRNAs by in situ hybridization in eight patients with dermatitis herpetiformis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Available plasmin , required for MMP zymogen activation , is regulated by plasminogen activators ( uPA , tPA ) and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect of UTI on tumor necrosis factor alpha ( TNF ) induced stimulation of urokinase type plasminogen activator ( uPA ) in cultured human umbilical vein endothelial cells ( HUVEC ) and in the promyeloid leukemia U 937 cells was studied . uPA antigen was evaluated in the cell lysate and in the conditioned media by enzyme linked immunosorbent assay , sodium dodecyl sulfate polyacrylamide gel electrophoresis , and Western blot . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) expression is induced upon cytoskeletal reorganization ( CSR ) by a mechanism independent of protein kinase C and cAMP protein kinase in nontransformed renal epithelial ( LLC PK 1 ) cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At the basolateral side of the cell , urokinase type plasminogen activator ( uPA ) bound to a specific cellular receptor is involved in the proteolytic modulation of matrix proteins and cell matrix interaction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Cysteine proteases [ cathepsin B ( CATB ) , cathepsin L ( CATL ) ] , the serine protease urokinase type plasminogen activator ( UPA ) and its inhibitor type 1 ( PAI 1 ) play an important part in cancer invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An increase of urokinase type plasminogen activator ( uPA ) and a decrease of tissue type PA ( tPA ) have been associated with the transition from normal to adenomatous colorectal mucosa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is thought to be an important mediator in the proteolytic degradation of extracellular matrix components observed in a wide variety of normal physiological and pathological conditions . ^^^ The peculiar predisposition of the uPA deficient animals to this rare bacterial infection and the development of phenotypic abnormalities associated with the targeted disruption the uPA gene suggests that uPA contributes significantly to the cutaneous microenvironment and is additional evidence of the extensive involvement of the plasminogen activators in mammalian physiology . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To know the effects of sex steroids on the potentials of growth , invasion , and metastasis with neovascularization of endometrial cancer , the expression of plasminogen activator inhibitor ( PAI ) 1 [ an inhibitor of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) ] and its mRNA in well differentiated uterine endometrial cancer cell line Ishikawa was determined by an enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction Southern blotting ( RT PCR SB ) , respectively , under the influence of sex steroids . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) is more important in modulation of fibrinolysis , and urokinase type plasminogen activator ( uPA ) is predominant in tissue remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine proteinase that has been suggested to play an important role in cancer invasion and metastasis . ^^^ It binds to a specific membrane receptor denominated uPA receptor ( uPAR ) . uPA activates plasminogen to form plasmin , which participates in tissue degradation and proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of urokinase type plasminogen activator ( u PA ) to a specific cell surface receptor ( uPA R ) has been shown to enhance plasminogen activation , a process involved in extracellular matrix degradation and cell migration during angiogenesis and tumor growth . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) binds to a specific cell surface receptor , uPAR . ^^^ The uPA catalyzed cleavage of uPAR is a new negative feedback regulation mechanism for cell surface plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Phenotype rasuPA+ / CL is dependent on expression of the serine type protease urokinase plasminogen activator ( uPA ) and the phenotype rasCL+ / uPA on the cystine type protease cathepsin L ( CL ) for lung colonization in experimental metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HGF / SF is responsible for various biological processes including angiogenesis and wound healing , in which secreted serine protease urokinase type plasminogen activator ( uPA ) is implicated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator ( uPA ) and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , appear to have a major function in these processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) is commonly overexpressed by many human cancers . ^^^ We used a mutated form of plasminogen activator inhibitor type 1 to conform a correlation between the inactivation of uPA and tumor size ; we have compared these results with the action of p aminobenzamidine and amiloride , known inhibitors of uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We previously demonstrated activation of matrix metalloprotease ( MMP ) and urokinase type plasminogen activator ( uPa ) expression in a coculture model consisting of squamous cell carcinoma cells ( SCC ) with dermal fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transgenic mice designated alpha MUPA overproduce in many brain sites the urokinase type plasminogen activator ( uPA ) , a protease implicated in fibrinolysis and extracellular proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition of urokinase type plasminogen activator ( uPA ) abrogates myogenesis in vitro . ^^^ Urokinase type plasminogen activator ( uPA ) is one of the components of blood ' s fibrinolytic cascade . uPA acts as a broad spectrum proteolytic enzyme involved in different physio pathological processes including cellular fibrinolysis , adhesion , migration , invasion and remodeling . ^^^ These results indicate that the increase in uPA expression prior to fusion and differentiation occurs via a MyoD mediated mechanism whereas the normal MyoD expression requires the plasminogen activation dependent activity of this protease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and 92 kDa matrix metalloproteinase ( MMP 9 ) expression by RAW264 . 7 macrophages were up regulated when plated on extracellular matrices . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is involved in the activation of different proteases which participate in the degradation of extracellular matrix , thereby enhancing the invasive capacity of tumour cells . uPA has been shown to be of prognostic importance in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast , inhibitors of urokinase type plasminogen activator ( uPA ) and / or plasminogen activation to plasmin ( aprotinin , amiloride , EACA , tranexamic acid , anti uPA antibody ) all reduced BMD by MDA MB 231 cells by approximately 30 40 % , but only in the presence of serum or plasminogen . ^^^ Combinations of BB 94 with any of the uPA / plasminogen activation inhibitors in serum containing medium had additive effects , while BB 94 with pepstatin and E 64 under serum free conditions reduced BMD to 16 % of control . ^^^ These in vitro observations suggest that combinations of proteinase inhibitors , particularly of uPA / plasminogen activation and MMPs , may merit clinical evaluation as potential antimetastatic therapy for breast cancer . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Epitope mapped monoclonal antibodies as tools for functional and morphological analyses of the human urokinase receptor in tumor tissue . uPAR ( CD 87 ) , the receptor for the urokinase type plasminogen activator ( uPA ) facilitates tumor cell invasion and metastasis by focusing uPA proteolytic activity to the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we study the influence of the protease urokinasetype plasminogen activator ( uPA ) and its receptor ( uPAR ) on melanoma cell adhesion to , and migration on , the extracellular matrix protein vitronectin ( VN ) . ^^^ In contrast , plasminogen activator inhibitor 1 ( PAI 1 ) can stimulate melanoma cell migration on VN , presumably by inhibiting uPA / uPAR mediated cell adhesion to VN and thereby releasing the inhibition of cell migration induced by uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator receptor ( uPA R ) : one potential characteristic of metastatic phenotypes in minimal residual tumor disease . ^^^ In parallel , accumulating data show functional association of the urokinase plasminogen activator ( uPA ) system and the membranous uPA receptor ( uPA R ) with the capacity of a tumor cell for invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The high level of soluble uPAR in PNH plasma competed with the membrane uPAR expressed by normal blood neutrophils for binding to urokinase type plasminogen activator ( uPA ) . ^^^ We also found that uPA complexed with soluble uPAR was partly resistant to inactivation by plasminogen activator inhibitors in the plasma , although uPA was inactivated similarly by plasma containing high and low levels of uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Deficiency of plasminogen or combined deficiency of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) was associated with severe functional and histological exacerbation of glomerular injury . ^^^ Deficiency of tPA , the predominant plasminogen activator expressed in glomeruli , also exacerbated disease . uPA deficiency reduced glomerular macrophage infiltration and did not significantly exacerbate disease . uPA receptor deficiency did not effect the expression of GN . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We also studied the relationship between the expression of MAGE 1 gene and the genetic expression of several tumor invasion related factors , including 72 kD type 4 collagenase ( MMP 2 ) , urokinase type plasminogen activator ( uPA ) , platelet derived growth factor A ( PDGF A ) , and vascular endothelial growth factor ( VEGF ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine whether the T cell lymphokines interleukin 4 ( IL 4 ) and interferon gamma ( IFN gamma ) modulate SMC fibrinolysis and migration induced by basic fibroblast growth factor ( bFGF ) , we examined the effects of IL 4 and IFN gamma on human SMC tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) antigen production , determined by enzyme linked immunosorbent assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The objective of this study is to determine the levels of matrix metalloproteinase 3 ( MMP 3 ) and urokinase type plasminogen activator ( uPA ) in knee synovial fluids from patients with rheumatoid arthritis ( RA ) and osteoarthritis ( OA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Migrating , undifferentiated myoblasts express urokinase plasminogen activator ( uPA ) and its cell surface receptor ( uPAR ) . ^^^ The function of uPA at this stage was tested using the protease inhibitors aprotinin , alpha 2 antiplasmin , or plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The different components of this system . e . g . urokinase plasminogen activator ( uPA ) , its receptor uPAR , as well as its main inhibitor plasminogen activator inhibitor type 1 ( PAI 1 ) have all been shown to have prognostic value in breast cancer , i . e . high tumor levels are associated with a poor prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
ELISAs for urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) have shown that tumor levels of these molecules are prognostic parameters in breast cancer as well as other types of cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Myosin binds both tPA and plasminogen , and enhances activation of des 1 77 plasminogen by tPA but not by urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This hypothesis is analogous to the concept proposed by Nykjaer et al in which plasminogen activator inhibitor 1 initially binds to uPA to form a complex that secondarily binds to the alpha 2 macroglobulin receptor , leading to internalization of the complex . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) initiates a proteolytic cascade with which invasive cells eliminate barriers to movement . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purpose of this study was to examine if the migration of human gingival fibroblasts on titanium was promoted by platelet derived growth factor ( PDGF ) and whether the release of urokinase type plasminogen activator ( uPA ) was correlated with it . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Considerable evidence links urokinase plasminogen activator ( uPA ) bound to its surface receptor ( uPAR ) with enhanced invasiveness of cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transformed endothelial ( End . ) cell lines established from such tumors exhibit altered proteolytic activity as a result of increased expression of urokinase type plasminogen activator ( uPA ) and are capable of inducing vascular tumors efficiently when injected into adult mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The two zymogens , plasminogen and pro urokinase plasminogen activator ( pro uPA ) , constitute a system of reciprocal activation , since plasmin , generated by uPA catalysed plasminogen activation , can activate pro uPA to uPA . ^^^ As an example of reciprocal zymogen activation , the plasminogen / pro uPA system was analysed in terms of a kinetic model which describes the progression in activated enzymes . ^^^ To distinguish between these possibilities the effect of a recombinant soluble ( residues 1 277 ) form of uPAR , uPAR ( 1 277 ) peptide , on reciprocal pro uPA / plasminogen activation was studied . uPAR ( 1 277 ) peptide attenuated reciprocal zymogen activation , and the results suggested that this was due to a decreased accessibility of the pro uPA / uPAR ( 1 277 ) peptide complex to activation by plasmin . ^^^ Kinetic analysis of separate activation steps revealed that this was due to a threefold stimulation of plasminogen activation by uPA / uPAR ( 1 277 ) peptide combined with a sixfold stimulation of plasmin ' s activation of pro uPA / uPAR ( 1 277 ) peptide . ^^^ The results suggested that poly ( D lysine ) provided a template for a catalytically favourable interaction between plasminogen / plasmin and the uPAR ( 1 277 ) peptide complex with pro uPA / uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of the present study was to examine the regulatory role of transforming growth factor alpha ( TGF alpha ) on urokinase plasminogen activator ( uPA ) gene expression and protein levels in hen granulosa cells from different stages of ovarian follicular development in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Low density lipoprotein receptor related protein ( LRP ) mediates the endocytosis of diverse ligands , including urokinase plasminogen activator ( uPA ) and its receptor , uPAR , which have been implicated in cellular migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is secreted from many cell types and localizes to focal contact areas when cells are seeded onto the plasma protein vitronectin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition of the interaction of urokinase type plasminogen activator ( uPA ) with its receptor ( uPAR ) by synthetic peptides . ^^^ Focusing of the serine protease urokinase type plasminogen activator ( uPA ) to the cell surface via interaction with its specific receptor ( uPAR , CD 87 ) is an important step for tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate the mechanisms by which NO controls angiogenesis , NO was assessed for the ability to affect cell proliferation and upregulation of urokinase type plasminogen activator ( uPA ) induced by basic fibroblast growth factor ( bFGF ) when added exogenously to or when produced endogenously by coronary venular endothelial cells ( CVECs ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) and the matrix degrading metalloproteinases MMP 2 and MMP 9 ( type 4 collagenases / gelatinases ) have been implicated in a variety of invasive processes , including tumor invasion , metastasis and angiogenesis . ^^^ The data reported in this paper show multiple roles of the uPA plasmin system in the control of gelatinase activity : ( 1 ) both gelatinases are associated with the cell surface ; binding of uPA and plasmin ( ogen ) to the cell surface results in gelatinase activation without the action of other metallo or acid proteinases ; ( 2 ) inhibition of uPA or plasminogen binding to the cell surface blocks gelatinase activation ; ( 3 ) in soluble phase plasmin degrades both gelatinases ; and ( 4 ) gelatinase activation and degradation occur in a dose and time dependent manner in the presence of physiological plasminogen and uPA concentrations . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Exogenous IGF 1 , urokinase type plasminogen activator ( uPA ) , UMR 106 conditioned media ( CM ) and PA 3 CM stimulated the proliferation of PA 3 and UMR 106 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Myogenic cell fusion was associated with increased cell associated urokinase type plasminogen activator ( uPA ) activity , cell associated uPAR , and uPAR occupancy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These data indicate that PAI 1 plays a direct role in dynamic cell adhesion particularly at the leading edge , where increased levels of urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) are localized in migrating cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A sensitive and robust assay for urokinase and tissue type plasminogen activators ( uPA and tPA ) and their inhibitor type 1 ( PAI 1 ) in breast tumor cytosols . uPA and PAI 1 are becoming established as amongst the most effective markers of poor prognosis for patients with node negative breast cancer ; tPA is an index of longer survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased urokinase plasminogen activator ( uPA ) levels are increased in a number of malignancies and have been correlated with decreased disease free interval and decreased overall survival . ^^^ Levels of uPA , urokinase plasminogen activator receptor ( uPAR ) and plasminogen activator inhibitor 1 ( PAI 1 ) were measured semiquantitatively in paraffin sections of tumours from 147 patients with NSCLC . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the ability of important regulators of osteoblast function , such as insulin like growth factor 1 ( IGF 1 ) , transforming growth factor beta 1 ( TGF beta 1 ) , and urokinase type plasminogen activator ( uPA ) to act as mediators in cell cell interactions between osteoblast like cells and metastatic prostate cancer cells , in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The GPI anchored urokinase plasminogen activator receptor ( uPAR ) does not internalize free urokinase ( uPA ) but readily internalizes and degrades uPA : serpin complexes in a process that requires the alpha 2 macroglobulin receptor / low density lipoprotein receptor related protein ( alpha2MR LRP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Production of a urokinase plasminogen activator IgG fusion protein ( uPA IgG ) in the baculovirus expression system . ^^^ Numerous studies have demonstrated the importance of urokinase plasminogen activator ( uPA ) and its receptor , uPAR , in the processes of tumor progression and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 2 ( PAI 2 ) is an important regulator of plasminogen activation , which inhibits both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mutational analysis of the genes encoding urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 in advanced ovarian cancer . ^^^ Evidence has accumulated that urokinase type plasminogen activator ( uPA ) , its inhibitor ( PAI 1 ) and receptor ( uPAR ) are involved in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Strong clinical and experimental evidence has accumulated that the tumor associated serine protease plasmin , its activator uPA ( urokinase type plasminogen activator ) , the receptor uPA R ( CD 87 ) , and the inhibitors PAI 1 and PAI 2 are linked to cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although tissue type plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) are believed to be involved in the biochemical cascade leading to extracellular matrix degradation during ovulation , the presence and possible role of urokinase type PA ( uPA ) and its receptor ( uPAR ) in follicular wall remodeling during follicular development are poorly understood . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fetal bovine aortic endothelial GM 7373 cells were transfected with a viral expression vector harboring the human urokinase type plasminogen activator ( uPA ) gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of the current study was to determine whether cathepsin D is a prognostic parameter for gastric carcinoma , and also to consider interaction with the urokinase plasminogen activator ( uPA ) system as an established risk factor for tumor associated proteolysis . ^^^ Plasminogen activator inhibitor type 1 as a representative of the uPA system was confirmed as a strong independent factor for disease free and overall survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Internalization of the urokinase type plasminogen activator ( uPA ) requires two receptors , the uPA receptor ( uPAR ) and the low density lipoprotein receptor related protein ( LRP ) / alpha2 macroglobulin ( alpha2M ) receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cytokines induce matrix metalloproteinases ( MMPs ) and urokinase type plasminogen activator ( uPA ) . ^^^ At different times after TMT injection , tissue samples from frontal lobe and hippocampus were assayed for MMPs and uPA , using gelatin substrate and casein / plasminogen substrate zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( UPA ) and its inhibitor PAI 1 are thought to play an important part in gastric cancer ( GC ) invasion and metastasis . ^^^ The concomitant activation of UPAR , UPA and PAI 1 in GC confirm the important role of the plasminogen activator system in the process of invasion and metastasis . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Glioblastomas extensively invade the surrounding normal brain tissue , with a concomitant expression of various proteolytic enzymes , in particular urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is an inducible extracellular serine protease implicated in fibrinolysis and in tissue remodeling . ^^^ Here , we tested whether the systemic treatment of mice with kainic acid ( KA ) , an amino acid inducing limbic seizures , could elevate in the brain mRNAs encoding uPA and its specific inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , a major antifibrinolytic agent . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression and localization of mRNA ' s for tissue plasminogen activator ( tPA ) , urokinase PA ( uPA ) , uPA receptor ( uPAR ) and inhibin subunits , alpha , beta A and beta B in monkey testes was investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two types of plasminogen activators ( PA ) , tissue type ( tPA ) and urokinase type ( uPA ) , were identified in the seminal plasma of both the human and the rhesus monkey . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A sandwich type ELISA has been developed for the assessment of complexes between urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) in extracts of squamous cell lung carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In 15 patients we investigated coagulation parameters before , during and post CPB , i . e . , fibrinogen , antithrombin ( AT ) 3 , thrombin antithrombin complex ( TAT ) , prothrombin fragments F 1 + 2 ( F 1 + 2 ) , factor ( F ) XIIa , tissue factor ( TF ) , and parameters of the fibrinolytic system , i . e . , plasmin antiplasmin complex ( PAP ) , D dimer , tissue plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The intrinsic activity of single chain pro urinary type plasminogen activator ( pro uPA ) and whether its receptor ( uPAR ) potentiates this activity remains controversial . ^^^ In this report , the pro uPA / uPAR ( 1 281 ) peptide complex in solution is shown to have equivalent plasminogen activator activity to that of active two chain uPA ( tc uPA ) . ^^^ The pro uPA / uPAR ( 1 281 ) peptide complex at 1 nM displayed similar activity to that of tc uPA for either [ Glu 1 ] plasminogen or [ Lys 77 ] plasminogen in chromogenic assays with Spl present as the plasmin substrate . ^^^ When assayed with another plasmin substrate , S 2251 , the pro uPA / uPAR ( 1 281 ) peptide complex was unable to activate plasminogen . ^^^ The pro uPA / uPAR ( 1 281 ) peptide complex and tc uPA also showed a similar extent of plasminogen activation as measured by SDS / PAGE , when incubated with plasminogen and Spl in the presence of 100 micro M aprotinin , and plasminogen activation by pro uPA alone was also stimulated in the presence of Spl in this assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In breast cancer , several investigations have demonstrated that the tumour biological factors uPA urokinase type plasminogen activator ) and its inhibitor PAI 1 are statistically independent , strong prognostic factors for disease free ( DFS ) and overall survival ( OS ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previous studies have demonstrated that overexpression of urinary plasminogen activator ( uPA ) in rat prostate cancer cells results in increased skeletal metastases , which are primarily of the osteoblastic variety . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In in vitro assays using bovine aortic endothelial cells , TZ 93 at non toxic doses inhibited cell migration , expression of urokinase type plasminogen activator ( uPA ) mRNA and uPA activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The levels of plasminogen activator urokinase ( uPA ) and of its inhibitor ( PAI 1 ) were measured by use of ELISA in the cytosol of tissue homogenates obtained from endometrial carcinomas and the marginal , tumor free endometrium of postmenopausal patients ( n = 64 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase ( urokinase plasminogen activator , uPA ) and its cell surface receptor ( uPA receptor , uPAR ) play an important role in a variety of physiological and pathological processes requiring cell migration and tissue remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVES : To evaluate the effect of therapeutic and pharmacologic concentrations of two non steroidal anti inflammatory drugs ( NSAIDs ) , nimesulide and naproxen , on the synthesis of urokinase ( uPA ) , plasminogen activator inhibitor ( PAI 1 ) and interleukin 6 ( IL 6 ) in human synovial fibroblasts isolated from osteoarthritis ( OA ) patients . ^^^ The suppressive action of the two drugs on the synthesis of uPA , while stimulating PAI 1 production , may have a positive impact on the balance of plasminogen activator / inhibitor , which could help reduce cartilage catabolism . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Detection of the receptor for the human urokinase type plasminogen activator using fluoresceinated uPA . ^^^ The urokinase type plasminogen activator ( uPA ) is a serine protease that plays a crucial role in blood coagulation and in tumor invasion and metastasis . uPA is a relatively large polypeptide and binds the uPA receptor ( uPAR ) with high affinity and specificity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) binds to a plasma membrane receptor ( uPAR ) that localizes plasmin generation to the cell environment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND & AIMS : Urokinase plasminogen activator ( uPA ) regulates plasmin generation from plasminogen . ^^^ METHODS : Using Northern blot analysis , in situ hybridization , and immunohistochemistry , the expression of uPA , its receptor ( uPAR ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and transforming growth factor beta 1 ( TGF beta 1 ) was studied in 14 patients undergoing pancreatic resection for CP . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombospondin 1 ( TSP 1 ) , through activation of transforming growth factor beta 1 ( TGF beta 1 ) , up regulates the main plasminogen activator , the urokinase type plasminogen activator ( uPA ) . ^^^ The objectives of this study were to determine the role of TSP 1 and TGF beta 1 in the localization of the plasminogen / plasmin system to the tumor cell surface by the uPA receptor ( uPAR ) and to determine its effect in breast tumor cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RNA was extracted from Day 15 . 75 pig embryos and uteri and reverse transcribed , and cDNA was amplified by polymerase chain reactions using primers specific for urokinase type plasminogen activator ( uPA ) , matrix metalloproteinases 2 and 9 ( MMP 2 and 9 ) , and tissue inhibitors of MMP 1 , 2 , and 3 ( TIMP 1 , 2 , and 3 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We show that protease ( s ) required in this process was synthesized and secreted by the sensitive cells and that antibodies against plasminogen activator urokinase ( uPA ) decreased the in vitro cytotoxicity of DT on Vero cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TCDD significantly increased mRNA levels for interleukin 1beta ( IL 1beta ) by 6h , and for urokinase plasminogen activator ( uPA ) and tumor necrosis factor alpha ( TNF alpha ) by 36h . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase plasminogen activator ( uPA ) is causally involved in cancer invasion and metastasis . ^^^ In the primary cancers , PAI 2 levels correlated weakly but significantly with those of uPA and PAI 1 , but not with tissue type plasminogen activator ( tPA ) or uPA receptor ( uPAR ) levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparisons with the structures of other serine proteinases that also possess Lys 156 , such as trypsin , factor Xa and human urokinase plasminogen activator ( uPA ) , identify a set of secondary interactions which are required for Lys 156 to fulfil this activating role . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In 587 frozen samples of malignant and nonmalignant tissue of breast , uterus , vulva , and ovary , levels of urokinase plasminogen activator ( uPA ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were examined with enzyme linked immunosorbent assay ( ELISA ) and cathepsin D ( cath D ) with radioimmunoassay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Patients with an elevated level of urokinase plasminogen activator ( uPA ) in breast cancer tissue have an adverse prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , urokinase type plasminogen activator ( uPA ) expression and tumor invasiveness did not correlate with MK expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) bound to a single class of site on VSMCs ( kd , 2 nmol / L ) , binding of pro uPA resulted in a large potentiation of plasmin generation and both were competed by antibodies to the uPA receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stimulation of EC with basic fibroblast growth factor ( bFGF ) resulted in increased expression of Hox D 3 , integrin alphavbeta 3 , and the urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We defined the role of urokinase plasminogen activator ( uPA ) and its growth factor like domain ( GFD ) in stimulating smooth muscle cell ( SMC ) migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study characterizes the expression of tissue type plasminogen activator ( TPA ) , urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) in hyperplastic vein grafts and normal venous tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A low M ( r ) plasminogen activator of the urokinase type ( uPA ) was secreted by epithelial cells recovered from the surface of preovulatory follicles ( Western blot analysis ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The internalization and degradation of cell associated urokinase type plasminogen activator ( uPA ) through the alpha 2 macroglobulin receptor / low density lipoprotein related receptor ( alpha2MR / LRP ) represent important steps in the control of plasmin formation . ^^^ The binding of scuPA to LM TK cells that lack the uPA receptor was stimulated by the hexapeptide EEIIMD , corresponding to amino acids 350 355 of plasminogen activator type 1 , which contacts the sequence RHRGGS , corresponding to amino acids 179 184 in uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To compare the plasminogen activators ( tPA , uPA ) and their inhibitors ( PAI 1 , PAI 2 ) at different gestational ages , related to levels in women at term and non pregnant women . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) binds to cells via a specific receptor attached to the plasma membrane by a glycosylphosphatidylinositol ( GPI ) anchor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Protease nexin 1 ( PN 1 ) is a serine protease inhibitor ( SERPIN ) that acts as a suicide substrate for thrombin ( Th ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PNS substrates which contain Lys in the P 1 position are specific for Lys plasmin and are either not hydrolyzed or hydrolyzed at a relatively low rate by factor Xa , thrombin , or urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a serine protease involved in pericellular proteolysis and tumor cell metastasis via plasmin mediated degradation of extracellular matrix proteins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to two chain urokinase ( uPA ) , a chemical conjugate between uPA and native saporin ( a cytotoxic plant seed ribosome inactivating protein ) did not require plasminogen activator inhibitors to be internalized . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To analyse the functional activity of the plasminogen activators urokinase ( uPA ) and tissue type plasminogen activator ( tPA ) in human synovial membrane , and to compare the pattern of expression between normal , osteoarthritic , and rheumatoid synovium . ^^^ The tissue distribution of uPA , tPA , uPA receptor ( uPAR ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) was studied by immunohistochemistry . uPA , tPA , uPAR , and PAI 1 mRNA values and mRNA distribution were assessed by northern blot and in situ hybridisations respectively . ^^^ In addition , in RA tissues , expression of the specific uPA receptor ( uPAR ) and of the plasminogen activator inhibitor type 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zimography demonstrated that both cell types expressed urokinase type plasminogen activator ( uPA ) . ^^^ Addition of plasminogen to the co cultures , which was likely to be activated to plasmin by uPA , resulted in activation of TGF beta and parallel stimulation of melanoma endothelium adhesion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of in vitro angiogenesis and upregulation of urokinase and tissue type plasminogen activator ( uPA , tPA ) expression are two hallmarks of vascular endothelial growth factor ( VEGF ) activity on cultured endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , in a defined cell free system , plasminogen activators [ uPA , tissue type plasminogen activator ( tPA ) , or streptokinase ] , in combination with one of a series of FSDs ( N acetyl L cysteine , D penicillamine , captopril , L cysteine , or reduced glutathione ] generate angiostatin from plasminogen . ^^^ The wild type recombinant plasminogen was converted to angiostatin in the setting of uPA / FSD ; however , a plasminogen activation site mutant and a catalytically inactive mutant failed to generate angiostatin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MC were found to react with antibodies against tissue type plasminogen activator ( tPA ) and urokinase receptor ( uPAR / CD87 ) , but not with antibodies against urokinase ( uPA ) or plasminogen activator inhibitors ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The relaxed conformation of plasminogen activator inhibitor 2 ( PAIr ) is formed during inactivation of the matrix degrading enzyme urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this work was to clarify the role of urokinase type plasminogen activator ( uPA ) on the profibrinolytic activity of heparin , chemically modified heparins [ partially : N desulfated ( N des ) , N desulfated N acetylated ( N des N ac ) , O desulfated ( O des ) , O / N desulfated N acetylated ( O / N des N ac ) ] and heparan sulfate . ^^^ Binding competition assays of plasminogen and uPA to heparin sepharose demonstrated that heparin bound to both enzymes . ^^^ Moreover , in the presence of increasing amounts of heparin , plasminogen activation mediated by uPA occurred as a bell shaped curve , suggesting the formation of a ternary complex . ^^^ In contrast , all chemically modified heparins lacked this cofactor activity , although N des and heparan sulfate partially retained the uPA binding capacity , and O des partially bound to both plasminogen and uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to basal invasion , which was urokinase ( uPA ) and plasmin dependent , RA enhanced invasion was dependent on tissue type plasminogen activator ( t PA ) and plasmin activity . ^^^ Enhanced invasion was not associated with significant changes in the expression of uPA or its membrane receptor UPAR ; plasminogen activator inhibitors PAI 1 and PAI 2 ; metalloproteinases MMP 1 , MMP 2 , MMP 3 , MMP 9 and membrane type MMP 1 ; or tissue inhibitors of metalloproteinases TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Finally , anti alphavbeta 3 monoclonal and polyclonal antibodies specifically inhibit mitogenesis and urokinase type plasminogen activator ( uPA ) up regulation induced by free FGF 2 in endothelial cells adherent to tissue culture plastic . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As implied by the name , uPAR was first identified as a high affinity cellular receptor for urokinase plasminogen activator ( uPA ) . ^^^ It mediates the binding of the zymogen , pro uPA , to the plasma membrane where trace amounts of plasmin will initiate a series of events referred to as `` reciprocal zymogen activation ' ' where plasmin converts pro uPA to the active enzyme , uPA , which in turn converts plasma membrane associated plasminogen to plasmin . ^^^ In addition to its role in plasminogen activation , compelling evidence has demonstrated a role for uPAR in cell cell and cell extracellular matrix adhesion , both directly and indirectly . uPAR is directly involved in binding to the extracellular matrix molecule , vitronectin , and the affinity of this binding is increased when uPAR is occupied by ( pro ) uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The complex of the type 1 plasminogen activator inhibitor ( PAI 1 ) and its target proteinases , the urokinase and tissue type plasminogen activators ( uPA and tPA ) , but not the free components , bind with high affinity to the endocytosis receptors alpha 2 macroglobulin receptor / low density lipoprotein receptor related protein ( alpha2MR / LRP ) and very low density lipoprotein receptor ( VLDLR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR / CD87 ) in cell migration and invasion is well substantiated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using a pregnant mare serum gonadotropin ( PMSG ) / human chorionic gonadotropin ( hCG ) induced rhesus monkey corpus luteum ( CL ) model , we have studied how urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) , are temporally expressed in CL of rhesus monkey at the luteotropic and luteolytic periods . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( UPA ) and its receptor are expressed in the vasculature and are involved in cell migration and remodeling of the extracellular matrix in the neointima . ^^^ UPA did not induce growth of endothelial cells , and tissue type plasminogen activator showed no activity on either cell type . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) system is of major biologic impact , but different interactive proteases and inhibitors with modulating effects also must be considered . ^^^ The expression of uPA , activators of uPA ( cathepsin D , antithrombin 3 ) , uPA substrates ( plasminogen , matrix metalloproteinase 2 [ collagenase 4 , 72 kD ; MMP 2 ] ) , uPA / plasmin inhibitors ( plasminogen activator inhibitor type 1 and 2 [ PAI 1 , PAI 2 ] , alpha 1 antitrypsin , alpha 2 antiplasmin ) , uPA receptor ( uPA R ) , and parameters of the uPA R cycle ( alpha 2 macroglobulin , alpha 1 antichymotrypsin ) could be determined immunohistochemically and were scored semiquantitatively in 203 patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown in NIH 3T3 fibroblasts that treatment with 12 O tetradecanoylphorbol 13 acetate ( TPA ) or fibroblast growth factor 2 ( FGF 2 ) activates the Ras / Erk signaling pathway in NIH 3T3 fibroblasts , leading to the induction of the urokinase type plasminogen activator ( uPA ) gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of urokinase plasminogen activator ( uPA ) to its specific receptor ( uPAR ) facilitates migration of vascular smooth muscle cells ( VSMC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate host and viral mechanisms determining hepadnaviral persistence and hepatocarcinogenesis , we developed a mouse model by transplanting woodchuck hepatocytes into the liver of mice that contain the urokinase type plasminogen activator transgene ( uPA ) and lack mature B and T lymphocytes due to a recombination activation gene 2 ( RAG 2 ) gene knockout . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The diuretic drug amiloride ( AMLD ) , which competitively inhibits the catalytic activity of urokinase plasminogen activators ( UPA ) , was used to study its effects on the proteolytic enzymes implicated in the invasiveness and metastases in a prostatic tumor model carrying two different sublines of adenocarcinoma of the prostate . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In studies reported here , we demonstrate that binding of both lipoprotein and non lipoprotein ligands to MSR A induced protein tyrosine phosphorylation and increased protein kinase C ( PKC ) activity leading to up regulated urokinase type plasminogen activator ( uPA ) expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate if neurotensin ( NT ) could induce activation of urokinase type plasminogen activator ( uPA ) in vascular endothelial cells , we utilized the acetyl NT ( 8 13 ) analogue , TJN 950 , in which the C terminal leucine is reduced to leucinol . ^^^ TJN 950 had no effect on expression of uPA , plasminogen activator inhibitor 1 or uPA receptor mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The interaction of urokinase type plasminogen activator ( uPA ) with its cell surface receptor ( uPAR ) is implicated in diverse biological processes such as cell migration , tissue remodeling , and tumor cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By using in situ hybridization , RNase protection assays , indirect immunofluorescence , Western blots , and bioassays , we show 1 ) the presence of insulin like growth factor 2 ( IGF 2 ) , IGF 2 receptor ( IGF IIR ) , IGF IR , TGF beta , plasminogen , plasminogen activators [ urokinase plasminogen activator ( uPA ) and tissue plasminogen activator ( tPA ) ] , and Cdk 4 in developing palates ; 2 ) on embryonic day 14 ( E 14 ) , which is a critical day for palatal growth , B10 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : Levels of p 53 , estrogen receptor ( ER ) , progesterone receptor ( PgR ) , and urokinase type plasminogen activator ( uPA ) were assayed in cytosolic extracts derived from primary tumors of 401 tamoxifen naive patients who developed recurrent disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , a correlation was seen between this phenotype and expression of urokinase ( uPA ) and tissue type ( tPA ) plasminogen activators ( P = 0 . 08 and 0 . 02 respectively ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The broad spectrum protease urokinase type plasminogen activator ( uPA ) has been implicated in muscle regeneration in vivo as well as in myogenic proliferation and differentiation in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , a member of the urokinase type plasminogen activator ( uPA ) system , can already be defined as an established new prognostic factor in gastric cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase receptor ( uPAR ) is a receptor for both urokinase plasminogen activator ( uPA ) and the adhesion protein vitronectin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In fact , in PC 3 and DU 145 cells but not in LNCaP cells , urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) are induced by bombesin treatment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor ( PAI ) 1 and 2 , and soluble uPA receptor ( suPA R ) concentrations were assayed by enzyme linked immunosorbent assay ( ELISA ) in the conditioned media . uPA and PAI 2 concentrations were not influenced by steroid treatment and did not differ between women with and without endometriosis , whereas PAI 1 was significantly up regulated by promegestone in both groups . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effects of transforming growth factor beta 1 ( TGF beta 1 ) and sodium butyrate on cell proliferation and the urokinase plasminogen activator ( uPA ) system were examined in normal human breast epithelial cells ( HBECs ) and in a breast cancer cell line , MDA MB 231 . ^^^ In HBECs , TGF beta 1 inhibited cell proliferation and uPA activity , while it augmented plasminogen activator inhibitor 1 ( PAI 1 ) antigen level . ^^^ Moreover , in the presence of plasminogen , cell detachment could be modulated by the level of cell associated uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The human transcription factor , UEF 3 , is important in regulating the activity of the urokinase plasminogen activator ( uPA ) gene enhancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transgenic mice were previously described , carrying the cDNA of the human or murine protease urokinase type plasminogen activator ( uPA ) while linked to the cell specific promoter of the alphaA crystallin gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Elimination of the Cys 558 Cys566 bond attenuated activation by urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) , but resulted in an increased susceptibility to cleavage by trypsin and plasma kallikrein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to basal invasion , which is plasmin , urokinase ( uPA ) , tissue type plasminogen activator ( t PA ) , matrix metalloproteinase ( MMP ) 9 and TIMP 1 inhibitable MMP dependent , TGFbeta 1 enhanced invasion is dependent upon plasmin and uPA activity but does not appear to involve t PA , MMP 9 or TIMP 1 inhibitable MMPs , as judged by inhibitor studies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MMP 2 expression was measured immunohistochemically and scored semiquantitatively ( score 0 3 ) in carcinoma cells , and results were correlated with clinicopathological tumor parameters and parameters of the urokinase type plasminogen activator ( uPA ) system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of proteolytic parameters of the urokinase type plasminogen activator ( uPA ) system [ uPA receptor ( uPA R ) , plasminogen activator inhibitor ( PAI ) 1 ] has been proven to be an independent prognostic parameter in cancer . ^^^ Therefore , in a consecutive prospective series of 203 gastric cancer patients , the expression of activators ( plasminogen , tPA , MMP 2 , cathepsin D , antithrombin 3 ) and inhibitors ( alpha 2 antiplasmin , alpha 2 macroglobulin , alpha 1 antitrypsin , alpha 1 antichymotrypsin ) of proteolysis was studied immunohistochemically in the tumor epithelium semiquantitatively ( score 0 3 ) in addition to the uPA system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
SPC upregulated cell surface plasminogen activity , and at the same time increased the cell surface expression of urokinase type plasminogen activator ( uPA ) and urokinase type plasminogen activator receptor ( uPA R ) in keratinocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this study was to quantify , by Northern analysis , the gene expression of urokinase plasminogen activator ( UPA ) , urokinase receptor ( UPAR ) and plasminogen activator inhibitor type 2 ( PAI 2 ) in human gestational tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied the urokinase type plasminogen activator ( uPA ) receptor ( uPA R ) in normal and neoplastic human thyroid cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor are localized in the vessel wall where they are involved in cellular activation and remodelling processes . ^^^ The formation of a ternary uPA / suPAR / VN complex on the cell surface and the free extracellular matrix could be inhibited by a monoclonal antibody against VN , as well as by plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Moreover , VN mediated binding of the uPA / suPAR complex led to a fivefold increase in plasminogen activator activity . ^^^ Through this novel pathway , VN concentrates the uPA / suPAR complex to cell surfaces and extracellular matrix sites , leading to the accumulation of plasminogen activator activity required for cell migration and tissue remodelling processes . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Autoactivation of avian urokinase type plasminogen activator ( uPA ) . ^^^ In contrast to mammalian urokinase type plasminogen activator ( uPA ) , which is produced and maintained in zymogen form , avian uPA is found in the active two chain form in cultures of normal and transformed chicken cells in the absence of plasmin , the putative natural activator of pro uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We measured the levels of p 53 and urokinase type plasminogen activator ( uPA ) in 634 tumor tissues from 634 different node negative primary breast cancer patients who underwent locoregional surgery in the Center Oscar Lambret between July 1989 and September 1994 . p 53 and uPA were assayed using commercially available kits in cytosols prepared for estradiol receptor ( ER ) and progesterone receptor ( PgR ) assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As interferons ( IFNs ) and dexamethasone can be used together with RA in the treatment of patients with APL , we have now studied the effects of RA together with IFNs and dexamethasone on the plasminogen activation cascade of these cells , including measurement of plasmin generation and uPA receptor ( uPAR ) , using enzyme immunoassays , fluorescence activated cell sorter analysis and RNA extraction with Northern blotting . ^^^ Our main results were : ( 1 ) plasmin was formed on the surface of APL cells ; ( 2 ) RA stimulated transiently plasmin generation and increased uPAR mRNA level ; ( 3 ) IFNs alpha and gamma potentiated RA in its effects on uPA and plasmin activities and on uPAR level ; ( 4 ) dexamethasone suppressed totally the effect of RA on uPA induction and plasminogen activation ; and ( 5 ) IFNs and dexamethasone alone did not have potent effects on plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have investigated the expression and cellular localization of urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator receptor ( uPAR ) , and plasminogen activator inhibitor 1 ( PAI 1 ) as well as plasminogen activation in rat liver regeneration by recruitment of progenitor ( oval ) cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of urokinase type plasminogen activator ( uPA ) to its cellular receptor ( uPAR ) renders the cell surface a favored site for plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The enhancement of tumor cell invasion was due to a stimulation of the proteolytic cascade of plasminogen activation since the effect required plasminogen activation and was abolished by inhibitors of urokinase type plasminogen activator ( uPA ) or plasmin . ^^^ They amplified plasminogen activation in culture supernatants up to 70 fold by stimulating ( 1 ) pro uPA activation by plasmin and ( 2 ) plasminogen activation by uPA . ( 3 ) In addition , sulfated polysaccharides partially protected plasmin from inactivation by alpha 2 antiplasmin . ^^^ Sulfated polysaccharides also stimulated tumor cell associated plasminogen activation , e . g . , ( 4 ) cell surface pro uPA activation by plasmin and ( 5 ) plasminogen activation by cell surface uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We present a systematic analysis of the sensitivity and specificity of immunohistochemical stainings for components of the plasminogen activation system , i . e . , uPA , tPA , PAI 1 , PAI 2 , and uPAR , on routinely processed ( formalin fixed , paraffin embedded ) tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of urokinase type plasminogen activator ( uPA ) to its receptor , uPAR , regulates cellular adhesion , migration , and tumor cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( UPA ) has been implicated in a broad spectrum of pathological processes e . g . cell adhesion , migration and proliferation and matrix remodeling that are considered important features of atherogenesis and plaque disruption . ^^^ UPA was detected in the extracts as pro UPA , UPA complexed to plasminogen activator inhibitor 1 , or as otherwise inactive UPA antigen , but not in the active two chain form . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MCF 7 cells were incubated on plastic or laminin coated wells , and medium and cell lysate aliquots were assayed for tissue type ( tPA ) and urokinase type plasminogen activator ( uPA ) by a chromogenic assay in combination with anti uPA antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Subsequently , antibodies against tissue type plasminogen activator ( tPA ) increased NO production in endotoxin treated mixed glial cell cultures while amiloride , an inhibitor for urokinase ( uPA ) , had no effect . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As indicated by zymography and the use of amiloride in the PA assay , the activity in the medium was primarily urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Synergistic transcriptional activation of the mouse urokinase plasminogen activator ( uPA ) gene and of its enhancer activator protein 1 ( AP 1 ) site by cAMP and retinoic acid . ^^^ We have investigated the mechanism whereby all trans retinoic acid ( tRA ) potentiates the 8 bromo cAMP ( 8 BrcAMP ) dependent transcription of the urokinase plasminogen activator ( uPA ) gene in SC 115 mouse mammary carcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and one of its inhibitors , the PAI 1 , are involved in the proteolytic cascade of matrix degradation during in vivo morphogenesis or metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Agonist activity of antiestrogen receptor complexes to regulate urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) endogenous gene expression in breast cancer cells . ^^^ We now describe the regulation of urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) endogenous gene expression by estradiol ( E 2 ) and different antiestrogens in BC 2 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The influence of antiestrogens on the release of plasminogen activator ( uPA ) by MDA MB 231 and MCF 7 breast cancer cells . ^^^ Aminocaproic acid , a specific plasmin inhibitor , produced a 50 60 % reduction in the degradation of labeled substrate , in the solid phase assay , using culture medium from both cell lines , thus indicating that most of the proteolysis observed was due to uPA mediated plasmin generation from plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We subjected the complex between the serine proteinase urokinase type plasminogen activator ( uPA ) and the serpin plasminogen activator inhibitor 1 ( PAI 1 ) to proteolytic digestion under nondenaturing conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Plasma tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and the plasminogen activator inhibitors ( PAI 1 and PAI 2 ) were evaluated and compared with plasma 17 beta estradiol levels , ranging from 20 pg / mL to > 5 , 000 pg / mL during the course of treatment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We measured the abundance and activity of urokinase plasminogen activator ( uPA ) , and the levels of PA inhibitor ( PAI 1 ) and insulin like growth factor 1 ( IGF 1 ) in conditioned media from both explants and osteoblast like cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Relationships were sought between PKC and the expression of E cadherin , alpha catenin , and vimentin , and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Association of urokinase type plasminogen activator ( uPA ) to cells via binding to its specific cellular receptor ( uPAR ) augments the potential of these cells to support plasminogen activation , a process that has been implicated in the degradation of extracellular matrix proteins during cell migration and tissue remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( UPA ) , particularly when bound to its receptor ( UPAR ) , is thought to play a major role in local proteolytic processes , thus facilitating cell migration as may occur during angiogenesis , neointima and atherosclerotic plaque formation , and tumor cell invasion . ^^^ This mutant UPA had similar plasminogen activator characteristics as wild type UPA , including its specific activity and interaction with plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , ras / ets 2 responsive genes such as the urokinase plasminogen activator ( uPA ) were activated in these four cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We also measured urokinase type PA ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) , which may not be endothelium derived but share major clearance pathways with tPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NF kappaB activation in fibroblasts was accompanied by induction of interleukin 6 ( IL 6 ) and urokinase plasminogen activator ( uPA ) , both of which promote angiogenesis and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study examines the effect of intracerebral infusion of thrombolytics , tissue plasminogen activator ( tPA ) , and urokinase ( uPA ) , individually and in combination with thrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) production by human breast ( myo ) fibroblasts in vitro : influence of transforming growth factor beta ( 1 ) ( TGF beta ( 1 ) ) compared with factor ( s ) released by human epithelial carcinoma cells . ^^^ The urokinase type plasminogen activator ( uPA ) may be considered as a key enzyme in the processes of cancer cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Amiloride is an inhibitor of urokinase plasminogen activator ( uPA ) , an essential component of the plasminogen / plasmin enzyme system . ^^^ Inhibition of uPA prevents the conversion of plasminogen to tumor cell surface bound plasmin which is required for initiation of the metastatic process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study examines the effects of prostaglandin I 2 ( PGI 2 ) on urokinase type plasminogen activator ( uPA ) production and wound healing by human fibroblasts . ^^^ Fibrin zymography , ELISA , and Northern blot analysis confirmed that the enhanced activity was caused by an increase in uPA synthesis and secretion and a decrease in type 1 plasminogen activator inhibitor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate the capacity of CHO SSF 3 cells for the efficient production of recombinant proteins in protein free media , expression plasmids containing either a human single chain urokinase type plasminogen activator ( uPA ) encoding cDNA or a humanized immunoglobulin G ( IgG ) kappa light chain cDNA were introduced by transfection . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease involved in cancer invasion and metastasis . uPA acts in vivo by binding to a membrane receptor known as uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The interactions of the secondary binding site of ecotin with bovine trypsin , rat trypsin and human urokinase type plasminogen activator ( uPA ) were investigated with alanine substitutions in ecotin at Trp 67 , Gly 68 , Tyr 69 , Asp 70 , Arg 108 , Asn 110 , Lys 112 and Leu 113 , which formed contacts between the inhibitor and protease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An approach using the three dimensional structure of the ecotin trypsin complex to guide combinatiorial design efforts was taken to create potent bidentate ecotin inhibitors for trypsin and human urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We report the same effect in murine antigen induced arthritis . uPA mediated plasminogen activation in arthritic joints may have deleterious effects via degradation of cartilage and bone matrix proteins as well as beneficial effects via fibrin degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Urokinase type and tissue type plasminogen activators ( uPA and tPA ) and PAI 1 were assayed in vascular SMC conditioned media and in cell lysates , using enzyme linked immunosorbent assay and western blotting . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Complexes between urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) were assessed in plasma and serum from 39 breast cancer patients and from 20 healthy individuals , applying a recently developed enzyme linked immunosorbent assay ( ELISA ) for the analysis of these complexes in tumor tissue extracts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a potentially important prognostic factor in breast cancer for identifying patients at high risk of recurrence . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have investigated cellular signalling events induced by urokinase type plasminogen activator ( uPA ) independent of its proteolytic activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of urokinase type plasminogen activator ( uPA ) to its receptor ( uPAR ) has been implicated in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To understand the hormonal regulation of the components of the plasminogen plasmin system in human breast cancer , we examined the oestradiol ( E 2 ) regulation of plasminogen activators ( PAs ) , namely urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and uPA receptor ( uPAR ) , in our model system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study we analyzed the expression and distribution of the urokinase plasminogen activator ( uPA ) , its membrane receptor ( urokinase plasminogen activator receptor [ uPAR ] ) , and its inhibitor ( plasminogen activator inhibitor 1 [ PAI 1 ] ) in acute necrotizing pancreatitis in human beings . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , we analyzed the expression of urokinase plasminogen activator ( uPA ) , urokinase plasminogen activator receptor ( uPAR ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) in samples of chronically rejected human kidneys . ^^^ CONCLUSION : The demonstrated up regulation of uPA / uPAR / PAI 1 in chronic renal rejection is consistent with the plasminogen / plasmin system contributing to tissue remodeling in this disorder . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Degradation of urokinase plasminogen activator ( UPA ) in endometrial stromal cells requires both the UPA receptor and the low density lipoprotein receptor related protein / alpha2 macroglobulin receptor . ^^^ Primary cultures of human endometrial stromal cells expressed a single class of specific high affinity binding sites for urokinase plasminogen activator ( UPA ) with a dissociation constant KD 1 . 0 nmol / l and saturation at 2 . 0 nmol / l . ^^^ Similar binding data and number of free binding sites , about 200 fmol / mg protein , were found for UPA in complex with its inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activators tPA and uPA , and their inhibitors , PAI 1 and PAI 2 , have been associated with epithelial homeostasis and wound healing . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cultured mesothelial cells produced tissue type plasminogen activator ( t PA ) , urokinase plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 and type 2 ( PAI 1 and PAI 2 ) during unstimulated conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) was the first protein shown to be UV inducible in xeroderma pigmentosum DNA repair deficient human cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To test the role of both systems , expression of fibrinolytic and gelatinolytic activity was quantified after vascular injury in mice with targeted inactivation of tissue type Plg activator ( tPA / ) , urokinase type Plg activator ( uPA / ) , or Plg ( Plg / ) . ^^^ Neointima formation 1 week after vascular injury was impaired in uPA / and Plg / mice compared with wild type ( WT ) mice or tPA / mice ( reduction of neointimal area to 30 % and 10 % of WT , respectively ) . ^^^ One week after injury of the femoral artery , tPA mediated fibrinolytic activity in arterial sections or extracts of WT , uPA / , or Plg / mice was not altered , whereas uPA activity levels in tPA / and Plg / mice were 2 to 3 fold higher than in uninjured controls . ^^^ Active MMP 9 represented 20 % to 46 % of total MMP 9 in WT , tPA / , and uPA / mice but was not consistently detectable in Plg / mice . ^^^ Thus , the unaltered ratios of active and latent MMP 2 suggest that proMMP 2 activation may occur in the absence of tPA , uPA , or Plg , whereas no active MMP 9 was detected in the absence of Plg . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine the level of expression and the significance of urokinase type plasminogen activator ( uPA ) and its plasminogen activator inhibitor type 1 ( PAI 1 ) in adenocarcinoma of the esophagus , we studied 54 tumor cases and a control group of normal gastric mucosa cases with ELISA using detergent extracted samples . uPA and PAI 1 were significantly elevated as compared to control tissue by factors of 16 and 14 , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator ( uPA ) mediates cancer invasion and metastasis by binding to a cell surface receptor ( uPA R , CD 87 ) on both tumor and stromal cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human lung microvascular endothelial cells ( HLMECs ) secreted 1 . 5 15 times more urokinase type plasminogen activator ( uPA ) antigen than human hepatic microvascular endothelial cells , human umbilical vein endothelial cells ( HUVECs ) , angioma endothelial cells , and lung fibroblasts . ^^^ All of these cells also secreted a 100 fold greater amount of plasminogen activator inhibitor 1 than of uPA antigen , and uPA activities were not detected in the culture medium . ^^^ The secretion of uPA and plasminogen activator inhibitor 1 from HLMECs was also enhanced by tumor necrosis factor alpha and IL 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) activates plasminogen to plasmin , which is involved in the degradation of the vascular basement membrane and extracellular matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasma urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and urokinase type plasminogen activator receptor ( uPAR ) levels were measured in healthy volunteers and breast cancer patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We report that purified recombinant SERP 1 forms SDS stable complexes with urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasmin , thrombin , and factor Xa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
SW 1116 invasion was quantitated by light microscopy and conditioned media were assayed by ELISA for MMP 1 , 2 , 3 , 9 ; TIMP 1 , 2 ; MMP / TIMP complex ; and urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown that thrombospondin 1 ( TSP 1 ) and TGF beta 1 upregulate the urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) and promote tumor cell invasion in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue extraction procedures for investigation of urokinase plasminogen activator ( uPA ) and its inhibitors PAI 1 and PAI 2 in human breast carcinomas . ^^^ Urokinase type plasminogen activator ( uPA ) and its inhibitors , plasminogen activator inhibitor type 1 ( PAI 1 ) and type 2 ( PAI 2 ) , are supposed to be involved in the expression of the invasive and metastatic phenotype of cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proximal tubules freshly isolated from rats treated for 2 d with lovastatin ( 4 mg / kg per d ) showed increased tissue type plasminogen activator ( tPA ) and urokinase ( uPA ) activities and antigens . ^^^ Incubation with lovastatin ( 5 microM ) of proximal tubules isolated from untreated rats induced an increase in tPA and uPA and a decrease in plasminogen activator inhibitor 1 ( PAI 1 ) activities . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study we sought to determine if the human pancreatic carcinoma cell line ASPC 1 produced enzymatic activity capable of converting plasminogen to angiostatin and to determine if urokinase plasminogen activator ( uPA ) is involved in this system . ^^^ The effect of uPA on angiostatin generation was determined by incubating plasminogen with antibody to uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Invasion and metastasis is aided by the secretion of guanidinobenzoatase , that cleaves the link peptide to fibronectin , and urokinase plasminogen activator ( uPA ) , which initiates a molecular cascade to activate plasmin and collagenases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
FGF 2 stimulated migration was blocked by antibodies against urokinase type plasminogen activator ( uPA ) or uPA receptor ( uPAR ) and by neutralizing anti HGF antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cells with low surface urokinase plasminogen activator ( uPA ) and uPA receptor ( uPAR ) were also incapable of intravasation , despite the presence of high levels of MMP 9 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In both cell systems , ets 2 phosphorylation was persistent , with maximal phosphorylation detected 8 to 24 h after CSF 1 stimulation , and was correlated with activation of the CSF 1 target urokinase plasminogen activator ( uPA ) gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present work examined whether an increase in the urokinase type plasminogen activator ( uPA ) and the plasminogen activator inhibitors ( PAIs ) is correlated with the natural history of cervical carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase plasminogen activator ( uPAR ; CD 87 ) is a 50 to 65 kDa glycosylphosphatidylinositol ( GPI ) anchored glycoprotein expressed by leukocytes and tumor cells where it facilitates uPA dependent , plasmin mediated pericellular proteolysis during cellular invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An essential step in the regulation of angiogenesis is the activation of urokinase ( urokinase type plasminogen activator , uPA ) , which in turn activates a cascade of proteases that play essential roles in endothelial cell migration and tissue remodeling . ^^^ Treatment of bovine capillary endothelial cells ( BBEC ) with 16K PRL inhibited bFGF stimulated urokinase activity in BBEC as detected by plasminogen substrate gel assay . 16K PRL did not appear to be acting via an effect on uPA expression because no change in messenger RNA levels were observed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Northern analysis of hypoxic murine lung demonstrated an increase in PAI 1 mRNA compared with normoxic controls ; in contrast , transcripts for both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) decreased under hypoxic conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have performed in situ expression analysis of urokinase type plasminogen activator ( uPA ) , as well as functionally related molecules ( uPA receptor , low density lipoprotein receptor related protein , plasminogen activator inhibitor type 1 ) in day 10 . 5 to 18 . 5 post coitum ( p . c . ) murine placentas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The activation of the zymogen plasminogen to the serine protease plasmin by urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators ( PA ) is an important event in a variety of physiologic and pathophysiologic processes in mammals . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To compare the prognostic impact of tumor angiogenesis factors ( vascular endothelial growth factor [ VEGF ] , angiogenin , and basic fibroblast growth factor [ bFGF ] ) , tumor proteolysis factors ( urokinase type plasminogen activator [ uPA ] and plasminogen activator inhibitor 1 [ PAI 1 ] ) , and conventional tumor markers ( stage , grade , and steroid receptors ) in early breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There was no significant relationship between either of the nm 23 isoforms and cathepsin D ( Cat D ) , urokinase plasminogen activator ( uPA ) , its inhibitor ( PAI 1 ) , steroid hormone receptors or ploidy status . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of the urokinase type plasminogen activator ( uPA ) mRNA , which is known to be correlated with cell migration and invasion , was enhanced in PU . 1 transfectants compared with mock transfectants . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human endometrial stromal cell cultures , stimulated for two days with recombinant transforming growth factor beta 1 ( TGFbeta 1 ; 10 ng / ml ) , contained conditioned medium concentrations of urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and uPA : PAI 1 complex . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mRNAs of ECM degrading components urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , matrix metalloproteinase 1 ( MMP 1 ) , and tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) , as well as TGFbeta 1 , bFGF , and FGFR , were detected during IVM in a factor specific pattern : all transcript levels found at COC 0 generally increased after 3 h of maturation and either remained high or decreased thereafter . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cytokine effects did not correlate with expression on astrocytes of laminin , fibronectin , tenascin , chondroitin sulphate proteoglycan , N cadherin , polysialyated NCAM ( PSA NCAM ) , tissue plasminogen activator ( tPA ) or urokinase ( uPA ) . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression in the liver of transgenic mice of a gene coding for a urokinase type plasminogen activator ( uPA mouse ) induces hepatotoxicity and prolonged post native liver regeneration from cellular clones which have inactivated the transgene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mutational studies previously showed that Asp 97 is crucial for the plasminogenolytic activity of TSV PA , here we identify the conservation of Asp 97 in both types of mammalian plasminogen activator tissue type ( tPA ) and urokinase type ( uPA ) . ^^^ It seems likely that Asp 97 of tPA and uPA will have a similar role in plasminogen recognition . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Autocrine factor and TNF alpha stimulated transcription of the invasiveness related protease plasminogen activator / urokinase mRNA ( upa ) with similar kinetics . ^^^ When N . 1 cells were exposed to purified plasminogen activator / urokinase protein ( uPA ) , cell matrix contact was disrupted . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The transcriptional localizations of urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) and its inhibitors ( PAI 1 and PAI 2 ) , which are possibly involved in cancer metastasis , have not been determined in human lung cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It is generated by the cleavage of the proenzyme plasminogen upon the action of the urokinase type plasminogen activator ( uPA ) . uPA is synthesized and secreted by tumor cells and normal cells and interacts with a specific cell surface receptor ( uPAR ) thereby focalizing enzymatic activity to the cell surface . ^^^ The activity of uPA is controlled by plasminogen activator inhibitors type 1 and type 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase plasminogen activator ( uPAR ) is a key molecule in cell surface directed plasminogen activation . uPAR binds urokinase plasminogen activator ( uPA ) and thereby focuses plasminogen activation on the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In gastric cancer , the urokinase type plasminogen activator ( uPA ) system plays important roles in invasion and metastasis , processes which entail proteolysis and adhesion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The recombinant catalytic domain of MMP 3 generated pro urokinase type plasminogen activator , whereas incubation of pro urokinase type plasminogen activator with the native forms of human or rabbit stromelysin 1 led to a moderate activation of pro uPA due to an additional cleavage that is catalyzed by a serine proteinase . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Studies on the relationship between urokinase plasminogen activator ( uPA ) and human sperm motility . ^^^ To clarify the role of urokinase plasminogen activator ( uPA ) in the mechanisms of regulating sperm motility and the ability of fertilizing , we investigated the quantities and activities of uPA in human seminal plasma and on the membrane of spermatozoa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , urokinase type plasminogen activator ( uPA ) expression in 150 potentially curatively resected SCCs of the esophagus was analyzed immunohistochemically by means of a murine monoclonal antibody ( American Diagnostica , Greenwich , CT ) and correlated with survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have reported previously that both urokinase type plasminogen activator ( uPA ) and its type 1 inhibitor ( PAI 1 ) are statistically significant prognostic variables in patients with high risk breast cancer ( Grondahl Hansen et al . , Cancer Res . , 53 : 2513 2521 , 1993 ) , and we recently described that the uPA receptor ( uPAR ) is a prognostic marker in postmenopausal , node positive breast cancer patients ( Grondahl Hansen et al . , Clin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Functional assembly of the plasminogen dependent proteolytic system on the cell surface requires multiple interactions involving urokinase ( uPA ) , urokinase receptor ( uPAR ) , plasminogen activator inhibitors , and other molecules that mediate cell migration and adhesion . ^^^ Our findings highlight the ability of uPAR to interact simultaneously with vitronectin and uPA in breast cancer , supporting a dynamic coupling of the molecular mechanisms underlying plasminogen dependent matrix degradation and cell adhesion . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have recently described the urokinase type plasminogen activator ( uPA ) and its type 1 inhibitor ( PAI 1 ) as strong prognostic variables in breast cancer ( J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ovarian cancer metastasis is associated with an increase in the urokinase type plasminogen activator ( uPA ) and its receptor uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
During EGF or SF induced invasion , UM SCC 1 cells expressed urokinase type plasminogen activator ( uPA ) and uPA receptor as well as the matrix metalloproteinases ( MMPs ) , membrane type MMP 1 , collagenase 1 , stromelysin 1 , and gelatinase B . ^^^ Despite the presence of a positive correlation between uPA receptor uPA expression and growth factor stimulated invasion , UM SCC 1 invasion was not affected by inhibitors directed against the plasminogen activator plasminogen axis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To appreciate the role of urokinase type plasminogen activator ( uPA ) in the activation of TGF beta , the production of total , active and latent TGF beta by explanted alveolar macrophages was compared in uPA deficient ( uPA / ) mice and their normal counterparts ( uPA+ / + ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The main enzymes involved in this process are urokinase type plasminogen activator ( uPA ) and the matrix metalloproteinases ( MMPs ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
External quality assessment of trans European multicentre antigen determinations ( enzyme linked immunosorbent assay ) of urokinase type plasminogen activator ( uPA ) and its type 1 inhibitor ( PAI 1 ) in human breast cancer tissue extracts . ^^^ High levels of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) in breast cancer tissue extracts have been associated with rapid disease progression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation by the urokinase type plasminogen activator ( uPA ) is facilitated in the presence of cells expressing the glycolipid anchored high affinity receptor for uPA ( denoted uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To test the hypothesis that urokinase type plasminogen activator ( uPA ) plays an important role in liver regeneration in vivo , partial hepatectomy was performed on wild type and uPA deficient ( uPA / ) mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The PAI 2 was originally detected in the human placenta , which inhibits urokinase type plasminogen activator ( uPA ) while it is unable to inhibit tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) may enhance matrix degradation as well as migration and invasion by smooth muscle cells ( SMCs ) , their roles in cell adhesion are uncertain . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) in tissue and serum of head and neck squamous cell carcinoma patients . ^^^ The aim of this study was to determine urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) concentrations in tumour and adjacent normal tissue samples from 58 patients , and in serum samples from 40 of 58 patients with squamous cell carcinoma of the head and neck obtained at diagnosis and after completion of therapy . uPA and PAI 1 serum concentrations were also measured in 28 healthy volunteers who served as controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present study examined mechanisms underlying this phenotypic change by investigating the effects of asbestos on transcription factor activation and expression of urokinase type plasminogen activator ( uPA ) and its receptor uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The addition of urokinase plasminogen activator ( uPA , 100 nM ) to HMC membranes induced the activation of pro MMP 2 via the activation of latent membrane associated MT 1 MMP as reflected by the cleavage of the 63 and 55 kD forms . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) are important in the regulation of tumor tissue progenesis , cell differentiation , tumor cell motility , and tumor cell invasiveness . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the effect of CsA therapy on the regulation of fibrinolysis in kidney graft recipients by measuring plasma concentration and activity of plasminogen activators ( tPA , uPA ) and their inhibitors ( PAI 1 , 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our results suggest that cell associated uPA plays a key role in regulating the amount of plasmin present at the surface of prostatic carcinoma ( PRCA ) cells and show that differential production of uPA corresponds with the capacity to bind and activate plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We showed that G CSF induced a dose dependent increase in the urokinase type plasminogen activator ( uPA ) activity in the conditioned medium of a PC 9 lung cancer cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , inhibition of JNK kinase ( JNKK ) in ras transformed cells with normally elevated JNK activity switches the protease dependent invasive phenotype from a urokinase plasminogen activator ( uPA ) dependent to a cathepsin L ( CL ) dependent invasive phenotype . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Given the trophic effects of relaxin on the pig uterus and cervix , the present study was designed to examine the impact of relaxin on urokinase and tissue type plasminogen activator ( uPA and tPA ) protein and activity in the uterus and cervix of prepubertal pigs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Despite similar chemical formulas , the inhibitors exhibit a range of diverse binding modes that reflect their inhibitory spectra against the serine proteinases trypsin , thrombin , factor Xa , tissue type plasminogen activator ( tPA ) and urokinase ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The possibility of dissociating the mitogenic activity of basic FGF ( FGF 2 ) from its urokinase type plasminogen activator ( uPA ) inducing capacity both at pharmacological and structural levels prompted us to evaluate the role of these autophosphorylation sites in transducing FGF 2 mediated uPA upregulation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Accumulated data indicate that endocytosis of the glycosylphosphatidyl inositol anchored protein urokinase plasminogen activator receptor ( uPAR ) depends on binding of the ligand uPA : plasminogen activator inhibitor 1 ( PAI 1 ) and subsequent interaction with internalization receptors of the low density lipoprotein receptor family , which are internalized through clathrin coated pits . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown that urokinase plasminogen activator ( uPA ) stimulates the growth of human keratinocytes in culture . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of the zymogenic form of urokinase type plasminogen activator ( pro uPA ) to its specific cellular receptor , uPAR , leads to a large potentiation of plasmin generation . ^^^ These lysine analogues were also found to compete a non active site interaction between uPA and plasminogen , detected by surface plasmon resonance ( Kd 50 nM ) , at concentrations correlating with their effect on cell surface plasminogen activation , suggesting that this interaction is part of the kinetic mechanism . ^^^ Consistent with this , synthetic peptides corresponding to the sequence uPA 149 158 ( GQKTLRPRFK ) and uPA 149 157 ( GQKTLRPRF ) specifically abolished the amplification of cell surface plasminogen activation . ^^^ These data demonstrate that a novel non active site interaction between uPA and plasminogen is necessary for the assembly and efficiency of cell surface plasminogen activation complexes . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Elevated levels of the urokinase type plasminogen activator ( uPA ) in tumor cells are conductive to tumor cell spread and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We hypothesized that urokinase type plasminogen activator ( uPA ) , a protease intimately involved in inflammatory cell migration and activation , is required for clearance of P . carinii . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study compared early and late radiation induced changes in endothelial TM , urokinase plasminogen activator ( uPA ) , and transforming growth factor beta ( TGF beta ) in normal rectum and tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By catalyzing plasmin formation , the urokinase type plasminogen activator ( uPA ) can generate widespread extracellular proteolysis and thereby play an important role in physiological and pathological processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor hypoxia and high levels of expression of the urokinase type plasminogen activator ( uPA ) receptor ( uPAR ) represent a poor clinical outcome for patients with various cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Incubation of endothelial cells with urokinase type plasminogen activator ( uPA , 1 nmol / L ) induced a rapid and pronounced increase in tyrosine phosphorylation of several proteins with a molecular weight between 80 to 90 and 130 to 140 kDa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) and matrix metalloproteinases ( MMPs ) 1 and 9 are considered to play important roles in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We recently proposed that a proteinase , urokinase type plasminogen activator ( uPA ) may be added to this list , based on correlative evidence linking expression of uPA and murine epidermal hyperproliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As a strategy to accelerate fibrinolysis , we generated recombinant adenoviruses containing human and mouse urokinase type plasminogen activator ( uPA ) cDNAs . ^^^ A single intratracheal instillation of these uPA containing adenoviruses into mouse lungs resulted in increased plasminogen activator activity in bronchoalveolar lavage fluid for at least 2 weeks . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In trophoblasts , the lack of JunB causes a deregulation of proliferin , matrix metalloproteinase 9 ( MMP 9 ) and urokinase plasminogen activator ( uPA ) gene expression , resulting in a defective neovascularization of the decidua . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We examined whether two newly defined parameters , the density of urokinase type plasminogen activator ( uPAD ) and the density of its receptor ( uPARD ) , which were determined by dividing the serum levels of uPA and uPAR by the prostate volume , respectively , could be used as predictors of the progression and prognosis of prostate cancer ( PC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MC were examined by Giemsa staining and by immunohistochemistry using antibodies against tryptase , chymase , tissue type plasminogen activator ( tPA ) , urokinase ( uPA ) , urokinase receptor ( uPAR ) , and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Finally , the expression of both urokinase plasminogen activator ( UPA ) and its receptor ( UPAR ) were induced after TPA treatment by 8 and 7 fold , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased expression and secretion of urokinase plasminogen activator ( uPA ) strongly correlates with the malignant phenotype of many types of cells , and the central role of uPA in tumor invasion is now well established . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine whether urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) directly modulate the migration of brain tumor cells , we examined six human brain tumor cell lines , 2 astrocytomas ( SW 1088 , SW 1783 ) , 2 medullobastomas ( Daoy , D341Med ) , and 2 glioblastomas ( U87MG , U118MG ) , for their surface uPAR expression , endogenous PA activity , and functional proteolytic activity by an ECM degradation assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The very low density lipoprotein receptor ( VLDLr ) binds diverse ligands , including urokinase type plasminogen activator ( uPA ) and uPA plasminogen activator inhibitor 1 ( PAI 1 ) complex . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These observations suggest a previously unconsidered role for urokinase type plasminogen activator ( uPA ) in T lymphocyte mediated immune responses . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Other proteases involved in cancer spread such as urokinase type plasminogen activator ( uPA ) and cathepsin D have previously been shown to be prognostic markers in breast cancer . ^^^ However , in multivariate analysis , using nodal status , tumor size , ER status and urokinase plasminogen activator ( uPA ) , CB was not an independent prognostic marker . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The zymography of tumor conditioned medium showed that the treatment of OMC 3 cells with EGF and TGF alpha resulted in increases of type 4 collagenase , stromelysin and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator system consists of two proteins : tissue plasminogen activator ( tPA ) and urokinase plasminogen activator ( uPA ) , which act upon their specific receptors to generate plasmin from plasminogen located on the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The very low density lipoprotein receptor ( VLDLR ) binds , among other ligands , the Mr 40 , 000 receptor associated protein ( RAP ) and a variety of serine proteinase serpin complexes , including complexes of the proteinase urokinase type plasminogen activator ( uPA ) with the serpins plasminogen activator inhibitor 1 ( PAI 1 ) and protease nexin 1 ( PN 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type and urokinase type plasminogen activators ( tPA and uPA ) distribute broadly in the brain . tPA and uPA exert a variety of functions during development . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) could be detected in the basal stratum of the cholesteatoma matrix and in the surrounding granulation tissue , while tissue type plasminogen activator ( tPA ) was not detectable at all . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In a collective of 112 node negative breast cancer patients , we compared the prognostic impact of HER 2 / neu gene amplification ( AMP ) determined by fluorescence in situ hybridization ( FISH ) and HER 2 / neu protein overexpression ( EXP ) measured by immunohistochemistry ( IHC ) with traditional prognostic factors ( tumor size , grade , steroid hormone receptor status , menopausal status ) and tumor invasion markers uPA ( urokinase type plasminogen activator ) and its inhibitor PAI 1 determined by enzyme immunoassay ( ELISA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Antisense uPAR inhibition decreased uPAR expression by 48 66 % and cell associated urokinase plasminogen activator ( uPA ) by 30 68 % . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase type plasminogen activator ( uPA ) plays an important role in the destruction of the extracellular matrix and basement membrane around cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ninety eight patients with histologically confirmed ovarian tumors ( 77 primary ovarian carcinomas of stages T 1 to T 3 according to the postoperative histopathological classification pTNM classification , 14 ovarian metastases of various origins and seven benign ovarian tumors ) were investigated with regard to the concentration of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor ( PAI 1 ) in membrane extracts of tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To address the hypothesis that the control of gene expression and the cellular proliferative response are specific to diseased and regenerative liver compartments independently , we assessed the expression of liver growth modulators , hepatocyte proliferation , and apoptosis in transgenic livers overexpressing the urokinase type plasminogen activator ( uPA ) . uPA livers have regenerative nodules that are visually distinct from the surrounding diseased compartments . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prognostic impact of urokinase type plasminogen activator ( uPA ) and its inhibitor ( PAI 1 ) in cytosols and pellet extracts derived from 892 breast cancer patients . ^^^ To evaluate the clinical relevance of urokinase type plasminogen activator ( uPA ) and its type 1 inhibitor ( PAI 1 ) measured by a recently developed enzyme linked immunosorbent assay ( ELISA ) , we analysed both components in samples derived from 892 patients with primary breast cancer ( median follow up 99 months ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mice deficient for the urokinase plasminogen activator ( uPA ) gene are deficient in the recruitment of T cells and macrophages and succumb to bacterial infections . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The active process of pericellular proteolysis is central in tumor invasion , and in particular the essential role of the urokinase type plasminogen activator ( uPA ) is well established . uPA mediated plasminogen activation facilitates cell migration and invasion through extracellular matrices by dissolving connective tissue components . uPA , its receptor ( uPAR ) and plasminogen activator inhibitor 1 ( PAI 1 ) are enriched in several types of tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both human and murine receptor antagonists showed significant inhibition of primary tumor growth , demonstrating that in vivo , both tumor and stromal cell uPA receptor dependent plasminogen activation can modulate tumor growth . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The PAI 2Delta65 / 87 deletion mutant retained inhibitory activity against its known serine proteinase target , urokinase type plasminogen activator ( uPA ) ; however expression of this mutant in HeLa cells failed to protect from TNF induced apoptosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We evaluated the prognostic impact of the protease urokinase plasminogen activator ( uPA ) and its inhibitor PAI 1 on overall survival in patients with advanced ovarian cancer stage FIGO IIIc in order to select patients at risk . uPA and PAI 1 antigen were determined by ELISA in primary tumour tissue extracts of 86 ovarian cancer patients FIGO stage IIIc enrolled in a prospective study . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Unlike PSA , hK 2 activates pro PSA , pro hK 2 and the zymogen form of urokinase type plasminogen activator ( uPA ) , an extracellular protease correlated with prostate cancer and metastasis . ^^^ We show here that hK 2 rapidly forms a complex with plasminogen activator inhibitor 1 ( PAI 1 ) , the primary inhibitor of uPA in tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of Met protein and urokinase type plasminogen activator receptor ( uPA R ) in papillary carcinoma of the thyroid . ^^^ HGF / SF has to be cleaved in its heterodimeric form by the urokinase type plasminogen activator ( uPA ) to become active as a ligand for Met receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using immunohistochemical techniques , the cellular localization and expression of urokinase type plasminogen activator ( uPA ) was assessed , whereas its activity was determined by fibrin zymography , and the amounts of enzyme were measured by an enzyme linked immunosorbent assay ( ELISA ) in primary spinal column tumors ( chordoma , chondrosarcoma , and giant cell tumor ) and metastatic tumors of the spine arising from various malignancies ( breast , lung , thyroid , and renal cell carcinomas , and melanomas ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Overexpression of urokinase plasminogen activator ( uPA ) has been proposed to be one of the mechanisms by which malignant glioma cells cause pericellular proteolysis of stromal structures during brain tissue invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ets 1 positively regulates expression of urokinase type plasminogen activator ( uPA ) and invasiveness of astrocytic tumors . ^^^ Urokinase type plasminogen activator ( uPA ) is often expressed in human tumors including astrocytic tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Several investigators have revealed that urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) are overexpressed in serum as well as in tumor tissues in patients with various types of cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TGF beta appeared to induce also membrane bound uPA activity and the release of active plasminogen activator inhibitor 1 , indicating that TGF beta has the potential to regulate plasminogen activation at the RPE cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present study compares plasma urokinase plasminogen activator ( uPA ) peptide levels , plasma plasminogen inhibitor ( PAI 1 ) activity and urokinase receptors ( uPAR ) on peripheral blood monocytes of patients with stable coronary artery disease ( SCAD ) and healthy volunteers . 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
ELISA for complexes of urokinase type and tissue type plasminogen activators with their type 1 inhibitor ( uPA PAI 1 and tPA PAI 1 ) . ^^^ An ELISA has been developed for the assessment of complexes between the urokinase type ( uPA ) and the tissue type plasminogen ( tPA ) activators with their inhibitor type 1 ( PAI 1 ) in cell culture medium and cytosolic extracts of breast tumours . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To evaluate whether cathepsin D , urokinase type plasminogen activator ( uPA ) , its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , or clinical factors can predict which patients are at risk for developing distant metastases after local recurrence ( LR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In HuSMC exposed to pulsatile flow ( 0 . 3 to 25 ml / min , 24 h ) , there was a flow dependent increase in migration ranging from a 1 . 3 + / 0 . 16 to 2 . 67 + / 0 . 26 fold increase , compared to static cells , concomitant with a significant increase in urokinase type plasminogen activator ( uPA ) mRNA levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The 55 kD urokinase ( uPA ) receptor ( uPAR , CD 87 ) is capable of binding uPA and may be involved in regulating cell associated plasminogen activation and pericellular proteolysis . ^^^ While investigating the relationship between uPAR levels and plasmin generation , we found that uPA catalyzed plasminogen activation is stimulated by cells which do not express uPAR . ^^^ The mechanism depends on simultaneous binding of both uPA and plasminogen to the cell and requires the presence of the amino terminal fragment ( ATF ) , available in single chain and two chain high molecular weight uPA , but not low molecular weight uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These phenotypic changes were associated with reduced expression levels of the endogenous urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) genes , the products of which are implicated in the control of cell adhesion and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , we showed that although the antigenic levels of PCI in two seminal plasma samples from patients with infertility were normal or slightly elevated , their inhibitory activities toward urokinase plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) were absent . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The reciprocal interaction between prostate cancer and bone stromal growth factors , including basic fibroblast growth factor ( bFGF ) , hepatocyte growth factor / scatter factor ( HGF / SF ) , and especially the insulin growth factor ( IGF ) axis initiates bone tropism , and is enhanced by prostate secreted endothelin 1 ( ET 1 ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Particular attention has been given to the effects of plasmin proteolysis which is generated in the extracellular matrix by urokinase plasminogen activator ( uPA ) complexed with its receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The presence of the two plasminogen activators ( PAs ) , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) , and their inhibitor type 1 ( PAI 1 ) in bone cells , suggests a role in one or more aspects of bone resorption such as osteoclast formation , mineral dissolution , and degradation of the organic matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Lalpha 42 and Lalpha 47 cells also demonstrated reduced susceptibility to the cytotoxin , Pseudomonas exotoxin A , and accumulated greatly increased levels of urokinase type plasminogen activator ( uPA ) in conditioned medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of urokinase plasminogen activator ( uPA ) by retinoic acid ( RA ) is the initial event preceding certain subsequent biological changes in vascular endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have analysed in vivo the 2 . 0 kb enhancer of the human urokinase type plasminogen activator ( uPA ) gene in HepG 2 cells , in which gene expression can be induced by phorbol esters . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown that urokinase type plasminogen activator ( uPA ) is highly expressed in murine C2C12 myoblasts and that antibodies against uPA are able to block both myoblast fusion and differentiation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Changes in urokinase plasminogen activator ( uPA ) , tissue like plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) immunoreactivity were also assessed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast , tissue type plasminogen activator ( tPA ) plasma levels appear unrelated to restenosis , and data regarding a possible role of urokinase type plasminogen activator ( uPA ) in circulation are not available at present . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We find that recombinant GDF 9 induces hyaluronan synthase 2 ( HAS 2 ) , cyclooxygenase 2 ( COX 2 ) , and steroidogenic acute regulator protein ( StAR ) mRNA synthesis but suppresses urokinase plasminogen activator ( uPA ) and LHR mRNA synthesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TGF beta 1 enhanced uPA production , the number of uPA cell surface binding sites , and the expression of the plasminogen activator inhibitor PAI 1 , in transformed PDV cells , but had no major effect on nontumorigenic MCA3D keratinocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) participate in matrix degradation and cell migration by focusing proteolysis and functioning as a signaling ligand / receptor complex . uPAR , anchored by a lipid moiety in the membrane , is thought to require a transmembrane adapter to transduce signals into the cytoplasm . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Analysis of the FGF 7 autocrine loop on the non tumorigenic epithelial cells PNT1A showed acquired invasive potential in in vitro extracellular matrix migration assays , specifically inhibited by an FGF 7 neutralizing antibody , and over expressed factors implicated in the migration process : the metalloproteinase MMP 1 and the plasminogen activator uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease causally involved in cancer invasion and metastasis . ^^^ Furthermore , uPA is prognostic in node negative patients , and a clinical trial is currently under way to assess whether uPA and its inhibitor , plasminogen activator inhibitor 1 , can differentiate between the majority of node negative breast cancer patients who are cured by surgery from the minority who might benefit from adjuvant therapy . uPA is also prognostic in other malignancies , such as gastric , colorectal , esophageal , renal , endometrial , and ovarian cancers . uPA may thus be a prognostic indicator for multiple types of adenocarcinoma . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cytosolic determinations of cathepsin D ( cath D ) , urokinase plasminogen activator ( uPA ) and its specific inhibitor PAI 1 have shown an association with adverse prognosis in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The recent immunohistochemical finding that invasive esophageal carcinomas express elevated levels of urokinase ( uPA ) and urokinase receptor ( uPA R ) in vivo suggest that the plasminogen activation system may contribute to metastasis in esophageal cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This is prevented by the MEK inhibitors PD 098059 and U 0126 , which also inhibit FGF 2 mediated upregulation of urokinase type plasminogen activator ( uPA ) and in vitro formation of capillary like structures in three dimensional type 1 collagen gel . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to define the relative contribution of the proteolytic domain and the receptor binding domain of urokinase plasminogen activator ( uPA ) toward its mitogenic properties we studied the effects of different uPA isoforms on migration and proliferation of human aortic smooth muscle cells ( hSMC ) . ^^^ Since the [ 3H ] thymidine incorporation response to each isoform occurred at concentrations ( > 50 nM ) much higher than necessary for uPAR saturation by ligand ( 1 nM ) , this mitogenic response may be independent of binding to uPAR . [ 3H ] thymidine incorporation responses to r uPA and uPAmut were sensitive to the plasmin inhibitor aprotinin , and uPA stimulated DNA synthesis was inhibited by plasminogen activator inhibitor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( uPA R ) focuses the proteolytic activity of its ligand , the urokinase type plasminogen activator ( uPA ) , on the cell surface , and can also act as an adhesion receptor for vitronectin ( VTN ) . uPA increases uPA R affinity for VTN and is also able to cleave its receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) activates the mitogen activated protein ( MAP ) kinases , extracellular signal regulated kinase ( ERK ) 1 and 2 , in diverse cell types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , we studied the effects of these CSFs on gelatinase production , urokinase plasminogen activator ( uPA ) production and their activity in human lung cancer cells . ^^^ Plasminogen activator zymographs of the conditioned media from the cancer cells showed that CSF treatment resulted in an increase in a 48 55 kDa plasminogen dependent gelatinolytic activity that was characterized as human uPA . ^^^ The conditioned medium from the cancer cells treated with CSFs stimulated the conversion of plasminogen to plasmin , providing a direct demonstration of the ability of enhanced uPA to increase plasmin dependent proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Factors reflecting two major aspects of tumour biology , invasion ( urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibiter ( PAI 1 ) , cathepsin D ) and proliferation ( S phase fraction ( SPF ) , Ki 67 , p 53 , HER 2 / neu ) , were assessed in 125 node negative breast cancer patients without adjuvant systemic therapy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purpose of this retrospective multicentre study was to assess the prognostic value of urokinase plasminogen activator ( uPA ) and p 53 levels in a large series of primary breast cancer , using an automatic quantitative luminometric method . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Macrophages concentrate urokinase type plasminogen activator ( uPA ) at the cell surface by expressing urokinase receptors ( uPAR ) in order to focus the pericellular space plasminogen dependent proteolysis important in matrix remodeling and cell movement . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PAI 2 mRNA levels generally varied inversely from those of its target , urokinase type plasminogen activator ( uPA ) , and the macrophage growth factor CSF 1 , which induces uPA , inhibited PAI 2 expression in cells treated subsequently with LPS . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunofluorescence analysis demonstrated strong cytoplasmic staining of CABA 1 cells with anti matrix metalloproteinase 9 ( MMP 9 ) and anti urokinase type plasminogen activator ( uPA ) antibodies . ^^^ By casein plasminogen zymography we observed high molecular weight ( HMW ) uPA and plasmin bands . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) and uPA receptor ( UPAR ) play important roles in the proteolytic cascade involved in the invasiveness of gliomas and other invasive tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Exogenously provided urokinase type plasminogen ( PLG ) activator ( uPA ) converts surface bound PLG to enzymatically active plasmin . ^^^ Radioactive release did not occur with untreated spirochetes or spirochetes treated with uPA or PLG alone . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ovarian carcinomas secrete single chain urinary type plasminogen activator ( scuPA ) and expression of uPA is up regulated relative to normal ovarian epithelium , leading to an enhanced proteolytic capacity which may facilitate invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To test this hypothesis , we analyzed the production of urokinase type plasminogen activator ( uPA ) , and its receptor ( uPAR ) , 2 molecules involved in the invasive phenotype , in cells over expressing RGD wild type FN ( FNwt clones ) or RGD mutated FN ( FN RGD minus clones ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : Urokinase type plasminogen activator ( uPA ) catalyses the formation of the proteolytic enzyme plasmin , which is involved in matrix degradation in the processes of tissue remodelling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were determined by the ELISA assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The prognostic impact of invasion markers PAI 1 and urokinase type plasminogen activator ( uPA ) on disease free survival ( DFS ) and overall survival ( OS ) in breast cancer has since been independently confirmed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The complex between urokinase ( uPA ) and its type 1 inhibitor ( PAI 1 ) is formed exclusively from the active forms of these components ; thus , the complex concentration in a biological sample may reflect the ongoing degree of plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Examination of transfectants for mRNA of a number of secreted proteases showed that only urokinase type plasminogen activator ( uPA ) expression was closely associated with OPN expression and cellular invasiveness . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI 1 ) , a member of the serine protein family , is the most active in vivo inhibitor of fibrinolysis induced by plasminogen , tissue plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The latter is a pathological disorder characterized by decreased urokinase type plasminogen activator ( uPA ) activity and fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have determined that KS cell cultures constitutively secrete multiple forms of several matrix metalloproteinases ( MMPs ) and an altered form of urokinase plasminogen activator ( uPA ) by zymogram and Western analysis of the culture media . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Studies from our laboratory have shown that exposure of human lung epithelial cells to urokinase plasminogen activator ( uPA ) induces their proliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its specific receptor ( uPAR ) act in concert to stimulate cytoplasmic signaling machinery and transcription factors responsible for cell migration and proliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Osteoblasts modulate secretion of urokinase type plasminogen activator ( uPA ) and matrix metalloproteinase 9 ( MMP 9 ) in human prostate cancer cells promoting migration and matrigel invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have investigated the in vivo and in vitro regulation of the human urokinase type plasminogen activator ( uPA ) gene by interleukin 1 ( IL 1 ) and analyzed the transcription factors and signalling pathways involved in the response of the 2 . 0 kb uPA enhancer to IL 1 induction and to tetradecanoyl phorbol acetate ( TPA ) induction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor cell invasion was assessed with a modified Boyden collagen type 1 invasion assay in the presence of TGF beta 1 , antibody to urokinase plasminogen activator ( uPA ) , or the selective cyclooxygenase 2 inhibitor SC 58125 . ^^^ Expression of uPA , uPA receptor , and plasminogen activator inhibitor 1 were determined by Western blot and enzyme linked immunosorbent assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of urokinase plasminogen activator ( uPA ) activity by E cadherin and hormones in mammary epithelial cells . ^^^ Urokinase plasminogen activator ( uPA ) is involved in proteolysis of extracellular matrix during development and tumor cell invasion . ^^^ In addition , the activation of plasmin from plasminogen was inhibited by anti E cadherin antibodies and PAI 2 , consistent with a role for uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We report that the urokinase type plasminogen activator ( uPA ) , one of the critical proteases involved in tumor invasion and metastasis , is overexpressed in pancreatic tumor cells and its overexpression is induced by constitutive RelA activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Overproduction of urokinase type plasminogen activator ( uPA ) and metalloproteases ( MMPs ) is strongly correlated with tumorigenicity and with invasive and metastatic phenotypes of human and experimental tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparison of the tumor associated proteases cathepsin D ( CATH D ) and urokinase type plasminogen activator ( uPA ) in cytosols of human breast cancer patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI ) 1 , a serine protease inhibitor , inactivates urokinase type plasminogen activator ( uPA ) and regulates degradation of the extracellular matrix ; whether it functions for or against tumor progression , however , has been the subject of controversy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previously , we showed that cytoskeletal reorganization ( CSR ) induced by colchicine or cyochalasins leads to activation of the urokinase type plasminogen activator ( uPA ) gene in LLC PK ( 1 ) cells via the Ras / Erk signaling pathway [ Irigoyen et al . ( 1997 ) J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the search for dissemination to a secondary site , cancer cells are capable of degrading most components of the extracellular matrix via an extracellular proteolytic cascade , including urokinase type plasminogen activator ( uPA ) and the matrix metalloproteinases ( MMPs ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cytokine regulated urokinase type plasminogen activator ( uPA ) production by human breast fibroblasts in vitro . ^^^ It has been shown that , in breast stroma , urokinase type plasminogen activator ( uPA ) mRNA is predominantly expressed by myofibroblasts located at the invasive areas of the tumor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Among these , urokinase type plasminogen activator ( uPA ) has been studied in neoplastic and cultured brain cells extensively . ^^^ Immature cortical neurons were immunoreactive for uPA , its receptor , and its substrate plasminogen peaking at the end of postnatal week two , consistent with the postulated role in synaptogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) expression was determined by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vitro studies have demonstrated that the binding of urokinase type plasminogen activator ( uPA ) to its cell surface receptor ( uPAR ) greatly accelerates plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) system plays an important role in tumor cell invasion , metastases , and angiogenesis . uPA , uPA receptor , and plasminogen activator inhibitor 1 ( PAI 1 ) are prognostic factors in different solid tumors , e . g . , renal cell carcinomas ( RCCs ) . von Hippel Lindau ( VHL ) disease is an inherited cancer syndrome that is characterized by extensively vascularized tumors , including hemangioblastomas and RCCs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( UPA ) which is a serine protease may play a key role in the processes of tumor invasion and metastasis since it converts plasminogen to plasmin and initiates a cascade of proteolytic events that lead to the degradation of extracellular matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study used mice with modifications of the thrombomodulin ( TM ) gene , the tissue type plasminogen activator ( tPA ) gene , and the urokinase type plasminogen activator ( uPA ) gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cysteine proteases [ Cathepsin B and L ( CATB , CATL ) ] and the serine protease urokinase type plasminogen activator ( UPA ) with its inhibitor type 1 ( PAI 1 ) are thought to play an important part in colorectal cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In gastric cancer , overexpression of the receptor ( uPAR or CD 87 ) for the serine protease urokinase type plasminogen activator ( uPA ) in disseminated cancer cells indicates shorter survival of cancer patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of the latent forms of matrix metalloproteinase 2 ( MMP 2 ) , MMP 3 and MMP 9 , urokinase plasminogen activator ( uPA ) , tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) and TIMP 2 expression were observed in both tumour and normal tissue samples from breast and colorectal tissue ; however , expression was greater in the tumour tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To identify regions responsible for ligand recognition , soluble receptor fragments containing the eight cysteine rich class A repeats were produced . ( 125 ) 1 RAP and ( 125 ) 1 labeled urokinase type plasminogen activator : plasminogen activator inhibitor type 1 ( uPA : PAI 1 ) complexes bound to the soluble fragment with K ( D , app ) values of 0 . 3 and 14 nM , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To study interactions between disease free survival ( DFS ) and four components of the plasminogen activator system : urokinase type plasminogen activator ( uPA ) , its two inhibitors ( PAI 1 and PAI 2 ) , and its membrane receptor uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Cathepsin B ( CATB ) and cathepsin L ( CATL ) , which are cysteine proteases , urokinase ( UPA ) and tissue type plasminogen activator ( TPA ) , both serine proteases , and their inhibitor type 1 ( PAI 1 ) are believed to play an important role in colorectal carcinoma ( CRC ) invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Insulin like growth factor 1 ( IGF 1 ) and urokinase type plasminogen activator ( uPA ) are inversely related in human breast fibroblasts . ^^^ Human breast fibroblasts have been shown to express urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine proteinase plasmin is , together with tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , involved in the dissolution of blood clots in a fibrin dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These observations led us to hypothesize that pulmonary fibrosis could be limited through enhancement of alveolar fibrinolytic activity by adenovirus mediated transfer of the urokinase type plasminogen activator ( uPA ) gene to the lung . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) activation of plasminogen is an important mediator of cell migration in many cell types . ^^^ In the developing avian heart , uPA has been implicated as a mediator of atrioventricular ( AV ) cushion cell migration ; however , the role of the plasminogen / plasmin system has not been examined . ^^^ The purpose of this study was to test the hypothesis that uPA conversion of plasminogen to plasmin mediates AV cushion cell migration in vitro . ^^^ Stage 17 / 18 chicken atrioventricular tissue lysates converted plasminogen into plasmin through uPA activity but no tissue type plasminogen activator activity was detected . ^^^ Although AV segments activated plasminogen and degraded extracellular proteins , uPA ' s functional role in cushion cell migration did not involve the plasminogen / plasmin system . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODOLOGY : We immunohistochemically examined 97 colorectal carcinomas for the expression of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) to investigate whether uPA and PAI 1 expressions could serve as prognostic parameters ; the gene expression of uPA and PAI 1 in human colon cancer tissues was also analyzed using reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
S 35 degradation could be inhibited by the plasmin inhibitor aprotinin and by anti human urokinase type plasminogen activator ( uPA ) antibody , indicating the involvement of the plasmin activation system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the role of insulin like growth factor 1 ( IGF 1 ) and urokinase plasminogen activator ( uPA ) in human osteoblasts from subchondral bone obtained from the tibial plateaus of OA patients and normal individuals . ^^^ The activity of uPA was evaluated by specific substrate hydrolysis and zymography under basal conditions and after plasminogen stimulation , in the presence and absence of added IGF 1 . ^^^ Addition of plasminogen promoted uPA activity in both normal and OA osteoblasts via a positive feedback loop due to plasmin generation , since this activity was inhibited by both PAI 1 and alpha 2 antiplasmin . ^^^ Unexpectedly , incubation with IGF 1 inhibited this positive feedback of plasminogen dependent uPA activity in OA osteoblasts , but not in normal osteoblasts , in a dose dependent manner . ^^^ Hence , normal osteoblasts were relatively insensitive to IGF 1 , whereas the same treatment reduced both uPA levels and plasminogen dependent uPA activity in OA osteoblasts while it increased basal uPA activity in OA osteoblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator receptor ( uPAR ) is a membrane anchored protein with urokinase plasminogen activator ( uPA ) as the ligand . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) converts plasminogen to plasmin and is highly chemotactic for many cell types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To test this hypothesis , we used complementary techniques in rabbit and human mesothelial cells to determine whether uPAR expression is altered by exposure to asbestos . uPAR expression was induced by chrysotile and crocidolite asbestos , but not by wollastonite , as indicated by binding of radiolabeled urokinase type plasminogen activator ( uPA ) to rabbit or human mesothelial cells . uPA was not induced by fiber exposure . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TPA treatment also causes secretion of urokinase type plasminogen activator ( uPA ) and expression of its receptor ( uPAR ) , which activates plasminogen to plasmin and may digest extracellular domains of desmosomes and hemidesmosomes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation by p 38 mitogen activated protein kinase of adenylate and uridylate rich element mediated urokinase type plasminogen activator ( uPA ) messenger RNA stability and uPA dependent in vitro cell invasion . ^^^ Their high invasiveness is thought to be due to constitutively high levels of urokinase type plasminogen activator ( uPA ) and its receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) interacts with its receptor ( uPAR ) to promote proteolysis as well as cell proliferation and migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There is evidence that serine proteases , such as tissue plasminogen activator ( TPA ) , urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor ( PAI ) , are involved in this process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Changes in the expression of this system , consisting of urokinase and tissue type plasminogen activators ( uPA and tPA , respectively ) , plasminogen activator inhibitors ( PAI 1 , PAI 2 ) and uPA receptor , have been associated with tumour aggressiveness in a variety of solid malignant tumours . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Gene expression for plasminogen activator inhibitor 1 ( PAI 1 ) , urokinase and tissue plasminogen activators ( uPA and tPA ) , urokinase plasminogen activator receptor ( uPAR ) , and transforming growth factor beta 1 ( TGF beta 1 ) was examined by Northern analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the production by leukaemic cells of plasminogen activators [ urokinase ( uPA ) and tissue type PA ( tPA ) ] , cell surface receptor for uPA ( uPAR ) and PA inhibitors ( PAI 1 and PAI 2 ) . ^^^ The finding of uPA , uPAR , PAI 1 and PAI 2 synthesized by leukaemic cells suggests that plasminogen activation may contribute to the invasive behaviour of these cells , the fibrinolytic imbalance observed in leukaemic patients and the differentiation and proliferation of M 4 M5 by interaction of uPA with uPAR . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vitro experimental evidences suggest that the proteolytic degradation of the extracellular matrix ( ECM ) by activation of the urokinase type plasminogen activator ( uPA ) / plasmin system may affect growth factor activity and bioavailability . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In RT PCR experiments , tissue type PA ( tPA ) mRNA levels in both aged hGF and rGF were higher than in young cells , whereas plasminogen activator inhibitor 1 ( PAI 1 ) mRNA remained unchanged and urotype PA ( uPA ) mRNA was not detected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Secretion of urokinase plasminogen activator ( uPA ) by ovarian surface epithelium ( OSE ) adjacent to the preovulatory ovine follicle has been implicated in apical tissue degradation and follicular rupture . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator uPA R can regulate integrin functions by associating with several types of beta subunit . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) , a critical component of the metastatic cascade , is also found at high concentrations in ovarian ascites and ovarian cancers , and the levels of uPA correlate inversely with prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Considering recent findings that both urokinase plasminogen activator receptor ( uPAR ) and plasminogen activator inhibitors ( PAIs ) are involved in tumor growth through an urokinase type plasminogen activator ( uPA ) activity independent mechanism , the relation between the presence of these factors in tumor tissue and the clinicopathologic variables in colorectal carcinoma was reevaluated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : In the present study , we determined the plasma concentrations of tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , PAI 2 , and uPA receptor ( uPAR ) in 25 patients with ovarian cancer , 16 patients with benign gynecologic tumor or inflammation , and 36 healthy controls in order to find out whether the plasma levels of these markers could be used to evaluate the prognostic value in patients with gynecologic cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The use of amiloride , a selective urokinase type plasminogen activator ( uPA ) inhibitor , shows that 90 % of this activity was due to a tissue type plasminogen activator ( tPA ) and 10 % to uPA whereas in the uterus 100 % of the activity was tPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Additionally , we considered the secretion of urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 and 2 ( PAI 1 and PAI 2 ) , as well as matrix metalloproteinases ( MMP ) 1 , 2 , 3 , and 9 , and their inhibitors TIMP 1 and TIMP 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its cell surface receptor ( uPAR ) have been shown to be expressed in macrophages in atherosclerotic arterial walls , but the regulatory mechanisms of their expression remain unclear . ^^^ LysoPC significantly increased the binding of the amino terminal fragment of uPA to the treated cells and the cell associated plasminogen activator activity . ^^^ The combined incubation with reduced glutathione diethyl ester or N acetylcysteine , antioxidants , suppressed the upregulation of uPA and uPAR mRNA and the increase in plasminogen activator activity by lysoPC . uPA and uPAR mRNA expression was also induced by the incubation with xanthine and xanthine oxidase , a superoxide anion generating system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The zymography of tumor conditioned medium showed that the treatment of OMC 4 cells with EGF and TGF alpha resulted in the increase of matrix metalloproteinase ( MMP ) 2 and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The attempts to identify initiation routes for activation of the proform of the key enzyme urokinase type plasminogen activator ( pro uPA ) in vivo have been hampered by the strong activator potency of the plasmin , that is generated during the progress of the cascade . ^^^ The urine from Plg / mice contained active two chain uPA as well as a proteinase capable of activating exogenously added pro uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In breast cancer , the expression of high protein levels of the insulin like growth factor 1 receptor ( IGF 1R ) and urokinase type plasminogen activator 1 ( uPA ) is strongly associated with breast cancer recurrence and decreased survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Its effects on two GPI anchored proteins [ the urokinase receptor , which binds urokinase type plasminogen activator ( uPA ) to cell surfaces , and alkaline phosphatase ] were also studied using bovine chondrocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the major circulating inhibitor of urokinase [ urokinase type plasminogen activator ( uPA ) ] , has been linked to the pathogenesis of lung cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously established that Hox D 3 is required for expression of integrin alphavbeta 3 and urokinase plasminogen activator ( uPA ) , which contribute to EC adhesion , invasion , and migration during angiogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The association between drug resistance and three markers for invasive capacity : cathepsin D ( Cath D ) , urokinase type plasminogen activator ( uPA ) and inhibitor of plasminogen activator type 1 ( PAI 1 ) was examined in nine cervical and laryngeal carcinoma cell lines resistant to different cytostatics . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our purpose was to analyze the predictive relevance of DNA ploidy , S phase fraction ( SPF ) , and tissue levels of lysosomal proteinases cathepsin D ( CD ) , cathepsin B ( CB ) , cathepsin L ( CL ) , and urokinase type plasminogen activator ( uPA ) and that of the intracellular cysteine proteinase inhibitor stefin A on clinical outcome . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HGF / SF induces the expression of urokinase plasminogen activator ( uPA ) and the uPA receptor ( uPAR ) , important mediators of cell invasion and metastasis . ^^^ We have developed a cell based assay to screen for inhibitors of this signaling system using the induction of endogenous uPA and uPAR and the subsequent conversion of plasminogen to plasmin as the biological end point . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Expression of transcripts and proteins of MMPs , TIMPs , and urokinase plasminogen activator ( uPA ) by cultured normal human conjunctival and conjunctivochalasis fibroblasts was determined by Northern hybridization , enzyme linked immunosorbent assay ( ELISA ) , and Western blot analysis , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To study this supposition , we investigated immunohistochemically the expression of tPA , uPA and its receptor , the plasminogen activator inhibitors PAI 1 and PAI 2 , tetranectin as well as the laminin breakdown as an event of secondary brain injury . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasma levels of urokinase type plasminogen activator ( UPA ) and the serum levels of cathepsin B were significantly increased in patients with gastrointestinal tumours . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The antigen levels of components of the urokinase type plasminogen activator ( uPA ) system of plasminogen activation are correlated with prognosis in several types of cancers , including breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To increase thrombolytic specificity of urokinase ( uPA ) , we engineered a recombinant chimeric plasminogen activator SZ51Hu scuPA , which consists of a humanized monoclonal antibody ( SZ 51Hu ) specifically against P selectin on activated human platelet and a single chain urokinase ( scuPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator receptor ( uPAR ) has been identified some 15 years ago and the anticipation was that its presence on the cell surface will provide a focus for anchoring uPA and possibly protect the enzyme from native inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Keratinocyte activation comprises changes in protein and gene expression pattern resulting in phenotypic and functional changes necessary for re epithelialization such as the expression of urokinase type plasminogen activator ( uPA ) and its cell surface receptor ( uPA R ; CD 87 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of the urokinase plasminogen activator ( uPA ) to its receptor ( uPAR ) regulates cell adhesion , surface proteolysis , chemotaxis and cell extravasation in a number of experimental systems . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the solid tumor , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and uPA receptor ( uPAR ) are considered as prognostic factors . ^^^ We have found that the plasminogen activation system ( uPA , PAI 1 , uPAR ) of tumor tissue is activated from the early stage of breast cancer . ^^^ The blood level of the plasminogen activation system correlated with that of tissue in an order of uPAR ( r ( 2 ) =0 . 61 ; P=0 . 001 ) , uPA ( r ( 2 ) =0 . 35 ; P=0 . 001 ) and PAI 1 ( r ( 2 ) =0 . 11 ; P=0 . 001 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Results obtained from in vitro experiments showed that urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) can cleave single chain HGF . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , it can regulate the expression of metalloproteinase genes such as collagenase 1 ( MMP 1 ) , stromelysin ( MMP 3 ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cathepsin B can degrade extracellular matrix proteins , such as collagen 4 and laminin , and can activate the precursor form of urokinase plasminogen activator ( uPA ) , perhaps thereby initiating an extracellular proteolytic cascade . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) plays a central role in many aspects of cellular regulation , such as fibrinolysis , cell migration , and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Preincubation of HSVECs with Bbeta 15 42 , a neutralizing antibody to urokinase plasminogen activator ( uPA ) , or the F ( ab ) ( 1 ) fragment of a monoclonal antibody to vascular endothelial cadherin significantly attenuated the increase in ICAM 1 stimulated by fibrinogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) , or a 7 residue peptide derived from this protein ( u 7 peptide ) , bound the receptor and stimulated apical endocytosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A study was performed to correlate the expression of junctional complex components ( such as zonula occludens 1 [ ZO 1 ] , a tight junction component protein ) and nonjunctional complex components ( such as urokinase type plasminogen activator [ uPA ] , a serine protease ; cathepsin L , a cysteine protease ; alpha 2 macroglobulin , a nonspecific protease inhibitor ; and cystatin C , a cysteine protease inhibitor ) at the time when inter Sertoli tight junctions were established in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue obtained during surgical debulking to control the edema showed very high levels of gelatinase B ( 92 kDa type 4 collagenase ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation of matrix metalloproteinase 2 ( MMP 2 ) and expression of urokinase type plasminogen activator ( uPA ) was observed with cellular invasion into matrix at the depth of 200 300 microm . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Macrophage colony stimulating factor ( CSF 1 ) binds to a receptor ( CSF 1R ) encoded by the c fms proto oncogene and activates transcription of the urokinase plasminogen activator ( uPA ) gene in murine bone marrow derived macrophages . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Experimentally induced pulmonary metastases of mammary cancer in the Fisher 344 rat can be suppressed by the inhibition of urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In situ hybridization and immunocytochemical localization were used to define the cellular and tissue distribution of urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and 2 ( PAI 2 ) and urokinase receptor in early monkey placenta and uterus . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) and tissue plasminogen activator ( tPA ) are very similar serine proteases with the same physiological function , the activation of plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The regulatory mechanisms underlying the overexpression of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) in highly invasive breast carcinomas remain poorly understood . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is thought to exert its effects on cell growth , adhesion , and migration by mechanisms involving proteolysis and interaction with its cell surface receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator system ( uPAS ) , consisting of the urokinase plasminogen activator ( uPA ) , the uPA receptor ( uPA R ) , and their corresponding inhibitors , PAI 1 and PAI 2 , is thought to play a role in this process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the last decade various groups have reported a strong and statistically independent prognostic impact of the serine protease uPA ( urokinase type plasminogen activator ) and its inhibitor PAI 1 ( plasminogen activator inhibitor type 1 ) in node negative breast cancer patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
After long term follow up , the prognostic impact of the following proteolytic factors associated with tumor invasion and metastasis was evaluated in 276 primary breast cancer patients : uPA ( urokinase type plasminogen activator ) , PAI 1 ( uPA inhibitor type 1 ) , and cathepsins B , D and L . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Overexpression of urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) has been well documented in a wide variety of tumor cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator , uPA , when bound to its specific receptor , uPAR ( CD 87 ) , plays a significant role in tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They were characterized by ( 1 ) growth kinetics ; ( 2 ) staining with monoclonal antibodies ( MoAbs ) to cytokeratin 19 , epithelial membrane antigen ( EMA ) , anticarcinoembryonic antigen ( CEA ) , breast cancer antigen 1 ( BRST 1 ) , breast cancer antigen 2 ( BRST 2 ) , Her2 / neu , and p 53 ; ( 3 ) expression of domains of urinary plasminogen activator ( uPA ) , neural cell adhesion molecule ( NCAM ) , and haptoglobin ( Hp ) ( Harvey et al . , 1997 ) ; and ( 4 ) karyotypic analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MDCK cells transformed by LMP 1 showed invasive growth to form branching tubules into collagen gel without HGF treatment . mRNA differential display and Northern hybridization identified plasminogen activator inhibitor 1 ( PAI 1 ) , urokinase type plasminogen activator ( uPA ) and ets 1 as genes upregulated during transformation by LMP 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) stimulates MCF 7 cell migration by binding to the UPA receptor and activating the Ras extracellular signal regulated kinase ( Ras ERK ) signaling pathway . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase type plasminogen activator ( uPA ) is a protease associated with tumor metastasis and invasion . ^^^ Therapeutically useful inhibitors must be selective for uPA and not appreciably inhibit the related , and structurally and functionally similar enzyme , tissue type plasminogen activator ( tPA ) , involved in the vital blood clotting cascade . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The interaction of fibrinolytic components with platelets or coagulation factors after endothelial injury , was investigated in mouse deficient in tissue type plasminogen activator ( tPA / ) , or urokinase ( uPA / ) and in their wild type control ( tPA + / + , uPA + / + ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urinary type plasminogen activator ( uPA ) expression and uPA receptor localization are regulated by alpha 3beta 1 integrin in oral keratinocytes . ^^^ Expression of urinary type plasminogen activator ( uPA ) and its receptor ( uPAR ) is correlated with matrix proteolysis , cell adhesion , motility , and invasion . ^^^ Coordinate regulation of the serpin plasminogen activator inhibitor 1 was also apparent ; however , a net increase in uPA activity was predominant . alpha ( 3 ) beta ( 1 ) integrin clustering induced extracellular signal regulated kinase 1 / 2 phosphorylation , and both uPA induction and extracellular signal regulated kinase activation were blocked by the mitogen activated protein kinase / extracellular signal regulated kinase kinase inhibitor PD 98059 . ^^^ As a functional consequence of uPA up regulation and uPA mediated plasminogen activation , the globular domain of the laminin 5 alpha ( 3 ) subunit , a major pp 126 matrix protein , was proteolytically processed from a 190 kDa form to a 160 kDa species . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The protein expression of tissue type plasminogen activator ( tPA ) was up regulated , while the free form of urokinase type plasminogen activator ( uPA ) was not detected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The protease , plasmin , can activate TGF beta , and activated T cells can express a receptor for the plasmin producing enzyme urokinase type plasminogen activator ( uPA ) , and can also produce both uPA and tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : We used a recently developed method for making precise distance measurements , based on donor donor energy migration ( DDEM ) , to accurately triangulate the position of the protease urokinase type plasminogen activator ( uPA ) in complex with the serpin plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased expression of the serine protease urokinase type plasminogen activator ( uPA ) in tumor tissues is highly correlated with tumor cell migration , invasion , proliferation , progression , and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) and plasminogen activator inhibitors ( PAI 1 and PAI 2 ) , all play important roles in tumour invasion and metastasis . ^^^ The tumour levels of the components of the urokinase type plasminogen activator system ( uPA system ) may help to identify individuals with a poor prognosis in postoperative non small cell lung cancer ( NSCLC ) patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These factors stimulate proliferation of vascular endothelial cells , the synthesis of proteases such as urokinase type plasminogen activator ( uPA ) and the matrix metalloproteases , which result in digestion of the extracellular matrix and allow endothelial cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor expression of com 1 mRNA , however , did not correlate with the mRNA levels for urokinase type plasminogen activator ( uPA ) , its receptor , or the type 1 inhibitor , which are factors that define a phenotype of tumor aggressiveness when elevated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Breakdown of purified ECM components or native ECM did not occur after exposure to untreated spirochetes or spirochetes treated with uPA or PLG alone . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we report that aggregated , but not nonaggregated , Abeta increases the level of the mRNAs encoding tissue plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , the levels of secreted plasminogen activator inhibitor 1 ( PAI 1 ) were markedly higher , while no apparent differences were seen in the levels of active uPA and tPa between EGF R ( / ) and wild type pancreata . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a prerequisite for tumor invasion . ^^^ The purpose of this study was to evaluate the effects of 4 HPR on uPA and plasminogen activator inhibitor 1 ( PAI 1 ) in prostate cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition of urokinase plasminogen activator ( uPA ) has been shown to suppress cancer cell invasion and metastasis in the laboratory setting by numerous investigators . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To evaluate the most effective factor in the invasion , metastasis and prognosis of hepatocellular carcinoma ( HCC ) , we examined urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor ( PAI ) 1 , PAI 2 and uPA activity by enzyme linked immunosorbent assays ( ELISA ) in HCC tissues obtained from 46 patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
High amounts of p 185 were significantly associated with a high expression of urokinase type plasminogen activator ( uPA ) ( P < . 010 ) , uPA receptor ( P = . 030 ) , type 1 plasminogen activator inhibitor ( PAI ) ( P < . 010 ) , type 2 PAI ( P = . 021 ) , cathepsin D ( P = . 036 ) , matrix metalloproteinase 2 ( P = . 024 ) , alpha 1 antichymotrypsin ( P = . 025 ) , and alpha 2 macroglobulin ( P = . 017 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transforming growth factor ( TGF ) alpha and urokinase type plasminogen activator ( uPA ) are examples of the genes up regulated posttranscriptionally by TCDD by mRNA stabilization . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since reported data have indicated that plasminogen activators ( uPA and tPA ) were able to excise the angiostatin fragment from the plasminogen parent molecule via plasmin generation , we determined levels of uPA and tPA and PAI 1 antigen in the conditioned media , and correlated the results with angiostatin generating capacity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The distances found were 57+ / 4 A and 63+ / 3 A , respectively , which is comparable to the distance obtained between the same residues when PAI 1 is in complex with urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor ( uPA R or CD 87 ) for the serine protease urokinase type plasminogen activator ( uPA ) plays a central role in invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It has become more and more clear in recent decades that the plasminogen activation system , which includes urokinase type plasminogen activator ( uPA ) , urokinase type plasminogen activator receptor ( uPAR ) , plasminogen activator inhibitor ( PAI ) 1 and PAI 2 , plays a very important role in the aggressiveness of cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We explored the role of urokinase and tissue type plasminogen activators ( uPA and tPA ) , as well as the uPA receptor ( uPAR ; CD 87 ) in mouse severe malaria ( SM ) , using genetically deficient ( / ) mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our present studies examine the catabolism of urinary plasminogen activator ( uPA ) PN 1 inhibitory complexes , which , unlike Th . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transformed PDV keratinocytes respond to TGF beta ( 1 ) by stimulating cell motility and invasiveness concomitantly to enhancement of the urokinase type plasminogen activator ( uPA ) expression / secretion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We demonstrate here that SPI 3 protein expressed by transcription / translation in vitro is able to form SDS stable complexes with the serine proteinases plasmin , urokinase type plasminogen activator ( uPA ) , and tissue type plasminogen activator ( tPA ) , consistent with inhibitory activity of the serpin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) have been used for thrombolitic therapy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A peptide derived from the nonreceptor binding region of urokinase plasminogen activator ( uPA ) inhibits tumor progression and angiogenesis and induces tumor cell death in vivo . ^^^ Urokinase plasminogen activator ( uPA ) plays an important role in the progression of several malignancies including breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In untreated animals both tPA and urokinase type plasminogen activator ( uPA ) activity was significantly increased within the region of infarct by 6 hours after reperfusion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Proteinase ( matrix metalloproteinase 1 ( MMP 1 ) , MMP 2 , MMP 3 , MMP 9 , urokinase type plasminogen activator ( uPA ) , and tissue type PA ( tPA ) ) and inhibitor ( tissue inhibitor of metalloproteinases ; TIMP 1 and TIMP 2 ) expression and proteinase activity were compared using substrate zymography , western blotting , immunohistochemistry , and quenched fluorescent substrate hydrolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The treatment of HSC 3 with anti vascular endothelial growth factor ( VEGF ) antibody or anti basic fibroblast growth factor ( bFGF ) antibody , either alone or in combination , attenuated the activity of urokinase type plasminogen activator ( uPA ) in the endothelial cells stimulated by the conditioned medium of HSC 3 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we determined if the urokinase type plasminogen activator ( uPA ) and / or the urokinase type plasminogen activator receptor ( uPAR ) were responsible for the invasion activity of a human glioma cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These factors include hepatocyte growth factor ( HGF ) and its receptor c MET , and urokinase plasminogen activator ( uPA ) and its receptor uPAR , basic fibroblast growth factor ( bFGF ) , TGF alpha and TGF beta 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cancer invasion and metastasis is a process requiring a coordinated series of ( anti ) adhesive , migratory , and pericellular proteolytic events involving various proteases such as urokinase type plasminogen activator ( uPA ) / plasmin , cathepsins B and L , and matrix metalloproteases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A high affinity receptor for urokinase type plasminogen activator ( uPAR ) has been identified on the plasma membrane of a number of different cell types , and has been shown to be important for plasminogen activation , cell adhesion , and possibly signal transduction . uPAR and uPA cosediment with secretory vesicles and specific granules by subcellular fractionation and translocate to the plasma membrane upon activation of neutrophils . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND AND OBJECTIVES : Urokinase type plasminogen activator ( uPA ) regulates a variety of processes involved in tissue morphogenesis , cell differentiation , migration and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPA R ) and inhibitors ( PA 1 1 ) expression in hepatocellular carcinoma in relation to cancer invasion / metastasis and prognosis ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) system has been widely associated with the development of breast carcinoma . ^^^ We studied the expression of uPA , urokinase plasminogen activator receptor ( uPAR ) and plasminogen activator inhibitor type 1 ( PAI 1 ) in human breast carcinomas and their bone metastases , using in situ hybridisation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) have been reported as prognostic factors in breast cancer patients and plasminogen activation is regulated by various factors such as uPAR and growth factors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cJun N terminal kinase ( JNK ) signaling pathway mediates induction of urokinase type plasminogen activator ( uPA ) by the alkylating agent MNNG . ^^^ We demonstrate that the urokinase type plasminogen activator ( uPA ) gene product , a broad spectrum extracellular protease to which no DNA repair function has been assigned , is transcriptionally induced by MNNG in C2C12 and NIH3T3 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Generation of the serine proteinase plasmin from the extracellular zymogen plasminogen can be catalyzed by either of two other serine proteinases , the urokinase and tissue type plasminogen activators ( uPA and tPA ) . ^^^ The plasminogen activation system also includes the serpins PAI 1 and PAI 2 , and the uPA receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
For this purpose , the presence and enzymatic activity of matrix metalloproteinases ( MMP 2 , MMP 9 ) , tissue inhibitors of MMPs ( TIMP 1 , TIMP 2 ) , urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators ( PAs ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were quantified by western blot and gelatin or plasminogen casein zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVES : Increases in urokinase plasminogen activator ( uPA ) have been reported in tissues undergoing remodelling , but its effects on the vessel intima formation are not known . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this study was to analyse the distribution pattern and activity level of urokinase type ( uPA ) and tissue type plasminogen activators ( tPA ) in normal skin and in tissue biopsies of progressing stages of CVI , prior to and including venous ulceration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The assay is based on activation of plasminogen by human urokinase type plasminogen activator ( uPA ) and simultaneous measurement of generated plasmin with the specific plasmin substrate H D Val Phe Lys 4 nitroanilide ( S 2390 ) , using purified native rat plasminogen for calibration . ^^^ The assay is suitable for accurate measurement of plasminogen in samples obtained from animals containing pharmacological concentrations of uPA or tissue type plasminogen activator ( tPA ) in their plasma when in vitro plasminogen activation is blocked at pH 5 by collecting blood in acidic anticoagulant . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) in fibrinolysis remains unsettled . ^^^ In contrast , uPA ( / ) and tissue type plasminogen activator tPA ( / ) mice , but not uPAR ( / ) mice , showed a marked impairment in pulmonary fibrinolysis throughout the experimental period . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of inflammatory cytokines in modulating expression of MMPs , their tissue inhibitors ( TIMPs ) , and urokinase plasminogen activator ( uPA ) as potential contributors to the pathogenesis of conjunctivochalasis was investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OSM stimulated increased mRNA levels of urokinase plasminogen activator ( uPA ) and urokinase plasminogen activator receptor ( uPAR ) in a time and dose dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a potent catalyst of extracellular proteolysis , which also binds to a high affinity plasma membrane receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor ( uPAR ) binds the urokinase type plasminogen activator ( uPA ) and facilitates a proteolytic cascade focused at the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interestingly , in cell free solutions , maspin does not inhibit several serine proteases including tissue type plasminogen activator and urokinase type plasminogen activator ( uPA ) . ^^^ In our experimental system , we chose prostate carcinoma DU 145 cells because these cells mediate plasminogen activation primarily by uPA , as shown by two different colorimetric enzyme activity assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We compared two methods that measure plasminogen activator inhibitor ( PAI ) activity in plasma based on the ability of PAI to inhibit tissue plasminogen activator ( tPA ) or urokinase ( uPA ) in order to determine which method most accurately measures plasma PAI activity after stressors , like hemorrhage . ^^^ However , we measured a 10 fold difference in PAI activity as measured between assays , suggesting that some endogenous plasma constituents ( tPA , uPA , plasminogen or plasmin ) may interfere with the accurate determination of PAI activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator ( uPA ) , its inhibitor ( PAI 1 ) , and its receptor ( uPAR ; CD 87 ) facilitate cancer cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this paper we describe the expression of the tissue plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and the uPA receptor ( uPAR ) , in normal and atheromatous human vascular tissue obtained at coronary and peripheral vascular surgery . tPA , uPA , PAI 1 and uPAR antigens were localised by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since the urokinase type plasminogen activator ( uPA ) initiates this cascade , this hypothesis was tested by investigating the effects of noncytotoxic levels of nickel subsulfide on the balance of uPA expression relative to expression of its inhibitor , PAI 1 , in cultured human bronchial epithelial cells ( BEAS 2B ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although fragments of the extracellular matrix component hyaluronan induce macrophage production of inflammatory mediators , the effect of hyaluronan on the fibrinolytic mediators plasminogen activator inhibitor ( PAI ) 1 and urokinase type plasminogen activator ( uPA ) is unknown . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) , plasminogen ( Plg ) , and plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) have been observed in many cancers and may contribute to progression and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We now report that tumor hypoxia in these patients is correlated with elevated antigen content of the tumor associated serine protease uPA ( urokinase type plasminogen activator ) , a marker of tumor cell invasion and metastasis . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TS apparently decreased the cell associated urokinase type plasminogen activator ( uPA ) activity and matrix metalloprotease 1 ( MMP 1 ) activity even in the presence of anti bFGF IgG . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The generation of the broad specificity serine protease plasmin in the pericellular environment is regulated by binding of the urokinase type plasminogen activator ( uPA ) to its specific glycosylphosphatidylinositol ( GPI ) anchored cell surface receptor , uPAR . ^^^ This interaction potentiates the reciprocal activation of the cell associated zymogens pro uPA and plasminogen . ^^^ In both cell lines , GPI uPA activated cell associated plasminogen with characteristics both qualitatively and quantitatively indistinguishable from those of uPAR bound uPA . ^^^ By contrast , TM uPA activated cell associated plasminogen less efficiently . ^^^ This was due to effects on the K , for plasminogen activation ( which was increased up to five fold ) and the efficiency of pro uPA activation ( which was decreased approximately four fold ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We examined the effect of UTI on urokinase type plasminogen activator ( uPA ) expression in ovarian cancer cell lines , HOC 1 and HRA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) system has been implicated in tumor spread . ^^^ We have used immunohistochemistry to examine three components of this system , ie , uPA , uPA receptor ( uPAR ) , and plasminogen activator inhibitor 1 ( PAI 1 ) , in a pilot study on 142 cases of breast carcinoma . ^^^ Although patients with strong fibroblastic expression of uPA showed a tendency toward a shorter time to relapse , none of the plasminogen activator proteins were significantly associated with relapse free survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In both conditions the in growth of capillary like tubular structures into fibrin required cell bound urokinase type plasminogen activator ( uPA ) and plasmin activities . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purpose of the present study was to clarify the clinical significance of the combined expression of MMP 9 , MMP 3 , and urokinase type plasminogen activator ( uPA ) in colorectal cancer , and the role of MMP 3 or uPA expression as an activator for MMP 9 . ^^^ The following activation mechanism for proteinase might occur : uPA coexpressed with MMP 9 activated plasminogen , and plasmin activated proMMP 3 , which was secreted depending upon inflammatory infiltration , and then MMP 3 activated proMMP 9 , resulting in colorectal cancer progression and metastasis . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To assess the role of urokinase type plasminogen activator ( uPA ) and uPA receptor ( uPAR ) on the invasive potential of cancer cells , in vitro experiments were performed using two human gastric cancer cell lines , NUGC 3 and MKN 28 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate whether the course of primary melanoma disease correlates with expression of the various components of the proteolytic plasminogen activation ( PA ) system , immunohistochemical stainings for activators of plasminogen ( tissue type ( tPA ) and urokinase type ( uPA ) ) , inhibitors of plasminogen activation ( type 1 ( PAI 1 ) and type 2 ( PAI 2 ) ) and the receptor for uPA ( uPAR ) were performed on 214 routinely processed melanoma lesions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human ovarian cancer cell line , HRA , was treated with phorbol ester ( PMA ) to evaluate the effect on expression of urokinase type plasminogen activator ( uPA ) , since the action of uPA has been implicated in matrix degradation and cell motility . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previous studies have shown that the adhesion protein , vitronectin , directs the localization of urokinase type plasminogen activator ( uPA ) to areas of cell substrate adhesion , where uPA is thought to regulate cell migration as well as pericellular proteolysis . ^^^ These data indicate that ligation of vitronectin integrin receptors is necessary but not sufficient for the localization of uPA to areas of cell matrix adhesion , and suggest that vitronectin may promote cell migration by recruiting vitronectin integrin receptors and components of the plasminogen activator system to areas of cell matrix contact . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of urokinase type plasminogen activator ( uPA ) to its receptor ( uPAR / CD87 ) regulates cellular adhesion , migration , and tumor cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zymography revealed diminished activity of urokinase type plasminogen activator ( uPA ) in FTC 236 and FTC 238 , but not in FTC 133 cultured with RA . mRNA expression of the uPA receptor was diminished in FTC 236 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate the prognostic significance of the plasminogen activation system in human chondrosarcoma , the immunohistochemical expression of urokinase type plasminogen activator ( uPA ) , urokinase type plasminogen activator receptor ( uPAR ) , plasminogen activator inhibitor , 1 ( PAI 1 ) and 2 ( PAI 2 ) were analyzed in 28 patients with chondrosarcoma . ^^^ These results demonstrated the usefulness of uPA , uPAR and PAI 2 expression as biological prognostic indicator and the importance of the plasminogen activation system in tumor progression and metastasis in chondrosarcoma . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this study was to determine the level of mRNA expression for the genes encoding urokinase ( uPA ) , urokinase receptor ( uPAR ) , and plasminogen activator inhibitor ( PAI 2 ) in endometrial carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : We compared the biological characteristics , that is , growth rate , motility , and invasive activity , of five ovarian cancer cell lines with the gene expression of various matrix proteases ( matrix metalloproteinase 1 [ MMP 1 ] , MMP 2 , MMP 9 , membrane type MMP type 1 [ MT 1 MMP ] , MT 2 MMP , MT 3 MMP , urokinase plasminogen activator [ uPA ] ) , their inhibitors ( tissue inhibitor of metalloproteinase type 1 [ TIMP 1 ] , TIMP 2 , plasminogen activator inhibitor type 1 , [ PAI 1 ] , and PAI 2 ) , and the potential transcriptional regulators E1AF and Ets 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vitro activation of recombinant MMP 2 is controlled by the activity of the urokinase type plasminogen activator ( uPA ) , which acts as a fibrin independent plasminogen activator . ^^^ METHODS : The expression of uPA , uPAR , the tissue type plasminogen activator , and plasminogen activator inhibitor ( PAI ) 1 and PAI 2 was investigated using reverse transcription followed by polymerase chain reaction and Western blotting . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , the role of the individual activators ( urokinase [ uPA ] and tissue plasminogen [ tPA ] activators ) and inhibitors ( plasminogen activator inhibitor [ PAI 1 ] ) of the fibrinolytic system in maintaining patency after coronary artery angioplasty and stenting is unclear . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We assessed plasminogen activator ( uPA and tPA ) and inhibitor ( PAI 1 ) expression in rat iliofemoral arteries after balloon injury using Western blot , enzyme activity , and quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrin autography showed that plasminogen dependent fibrinolytic activity occurred at M ( r ) of 110 kDa , which represents a complex of tPA with PAI 1 , and 65 and 55 kDa bands corresponding to tPA and uPA , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study was designed to investigate the relationship of urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) to invasion and metastasis of hepatocellular carcinoma ( HCC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Deprivation of these two amino acids decreased the secretion of urokinase plasminogen activator ( uPA ) and tissue plasminogen activator ( tPA ) while plasminogen activator inhibitor ( PAI ) 1 and 2 were increased in these cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Simultaneous up regulation of urokinase type plasminogen activator ( uPA ) and uPA receptor by hepatocyte growth factor / scatter factor in human glioma cells . ^^^ In this study , effects of HGF / SF on urokinase type plasminogen activator ( uPA ) mediated proteolysis network were examined in c Met positive human glioma cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A potential factor in the local regulation of tumor cell invasion is the urokinase type plasminogen activator ( uPA ) system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Coordinate induction of collagenase 1 , stromelysin 1 and urokinase plasminogen activator ( uPA ) by the 120 kDa cell binding fibronectin fragment in fibrocartilaginous cells : uPA contributes to activation of procollagenase 1 . ^^^ That the urokinase plasminogen activator ( uPA ) was involved in the activation of procollagenase 1 was suggested by findings that the 120 kDa Fn fragment induced uPA coordinately with procollagenase 1 , and the activation of procollagenase 1 was dose dependently inhibited in the presence of plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ceramide enhanced urokinase type plasminogen activator ( uPA ) release is mediated by protein kinase C in cultured microglia . ^^^ As described previously , a relatively high dose of neurotrophins increased the release of urokinase type plasminogen activator ( uPA ) from cultured microglia . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a serpin protease inhibitor that binds plasminogen activators ( uPA and tPA ) at a reactive center loop located at the carboxyl terminal amino acid residues 320 351 . ^^^ The interaction of `` inactive ' ' PAI 1 , rPAI 1 ( 23 ) , with plasminogen and uPA induces the formation of a proteolytic protein with angiostatin properties . ^^^ These two studies show that rPAI 1 ( 23 ) interactions with uPA and plasminogen can inhibit plasmin by two mechanisms . ^^^ In a second mechanism , rPAI 1 ( 23 ) can bind uPA and / or plasminogen to reduce the number of uPA and plasminogen interactions , hence reducing the amount of plasmin that is produced . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen ( uPA ) activator regulates a variety of processes , including morphogenesis , cell differentiation , migration , and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To define the interaction of fibrinolytic components with platelets or coagulation factors on thrombus formation , we investigated mouse deficient in tissue plasminogen activator ( tPA / ) or urokinase plasminogen activator ( uPA / ) and in their wild type control ( tPA + / + , uPA + / + ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study we verified this postulate and focused our attention on the quantification of the decay products of the urokinase type plasminogen activator ( uPA ) mRNA that belongs to short lived mRNAs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor ( uPAR / CD87 ) together with its ligand , urokinase type plasminogen activator ( uPA ) , constitutes a proteolytic system associated with tissue remodelling and leucocyte infiltration . uPAR is a member of the glycosyl phosphatidyl inositol ( GPI ) anchored protein family . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) may serve this function , as it is an initiator of the matrix proteolysis cascade and induces hepatocyte growth factor expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously investigated the role of the urokinase plasminogen activator ( uPA ) system in NK cell invasion . ^^^ Zymographic analyses detected uPA in RNK 16 cell conditioned media ( CM ) with the same molecular weights as the uPA we have previously shown to be associated with the rat NK cell urokinase plasminogen activator receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The level of synthesis of osteoblast biomarkers ( osteocalcin , alkaline phosphatase ) , as well as urokinase plasminogen activator ( uPA ) activity and insulin like growth factor ( IGF 1 ) in the culture medium , was estimated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Levels of tissue and urokinase plasminogen activator ( t PA and uPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and type 2 ( PAI 2 ) mRNA and protein were assessed by quantitative reverse transcriptase polymerase chain reaction ( Q RT PCR ) and ELISA , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Concentrations of the fibrinolytic components tPA and urokinase plasminogen activator ( uPA ) , tPA activity and plasminogen activator inhibitor type 1 ( PAI 1 ) concentration were measured by enzyme linked immunoabsorbent assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) , and its inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) , are proposed to be prognostic factors in some cancers . ^^^ METHODS : To determine the prognostic value of the urokinase plasminogen activation system , contents of uPA , uPAR , and PAI 1 were measured in extracts of endometrial cancer tissue using ELISAs . uPA , uPAR , and PAI 1 levels were determined in 91 , 54 , and 92 extracts , respectively , and correlated with tumor histology , stage , grade , lymph node involvement , prevalence of metastasis , and recurrence as well as with estrogen ( ER ) , progesterone ( PR ) , epidermal growth factor ( EGFR ) receptor and HER 2 / neu contents . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Among the proteases involved in the tumor invasion process , components of the plasminogen activator system ( plasminogen activator type urokinase uPA , its membrane receptor uPAR and its two inhibitors PAI 1 and PAI 2 ) appear to define high risk patients in primary breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Expression of transcripts and proteins of MMPs , TIMPs , and urokinase plasminogen activator ( uPA ) by cultured human pterygium head , body , and subconjunctival fibroblasts , and normal corneal and conjunctival fibroblasts were determined by Northern hybridization , enzyme linked immunosorbent assay , Western blotting , zymography , and quantitative collagenase assay , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The importance of plasminogen activation , mediated by urokinase ( uPA ) and its receptor ( uPAR ) , is well established in many physiologica and pathological processes , such as in cell migration and tumor cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have here studied the expression of the cytokine interleukin 1alpha ( IL 1alpha ) in 73 human bladder carcinomas in relation to patient survival , and examined possible relationships between IL 1alpha and urokinase plasminogen activator ( uPA ) expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To answer this question , we used limited proteolysis to detect conformational differences between free urokinase type plasminogen activator ( uPA ) and uPA in complex with plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An increase was also noted in plasminogen activator inhibitor type 1 ( PAI 1 ) mRNA levels ( 2 . 7 fold ) , but none was noted in uPA levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We define the expression of urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and type 2 ( PAI 2 ) , the receptor for uPA ( uPAR ) and fibrin / fibrinogen in monkey implantation sites . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Trimidox induces c myc expression , which is indispensible for apoptosis of N . 1 cells , and expression of plasminogen activator / urokinase type ( upa ) , which supports the apoptotic process under more physiological conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
AIMS : To plasminogen activator system ( PAS ) consists of the plasminogen activators ( urokinase ( uPA ) and tissue type ( tPA ) plasminogen activators ) , the uPA receptor ( uPAR ) , and the plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Considering the established role of urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) in invasion , this study was undertaken to explore the role of heregulin beta 1 in regulating uPA and uPAR in breast cancer invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HRG and PGE 2 each induced the formation of well organized , three dimensional structures with empty spaces in the center and stimulated the expression of urokinase plasminogen activator ( uPA ) with differential localization of membrane bound uPA at the focal adhesion points and leading edges of the motile cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , after the incubation of PAI 1 with SP 40 , 40 at 37 degrees C for 1 h , PAI 1 could still form a complex with tissue plasminogen activator ( tPA ) , and it inhibited plasmin formation in the mixture of plasminogen and urine plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The many actions of CSF 1 include induction of urokinase type plasminogen activator ( uPA ) , a serine protease involved in extracellular matrix degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Surface bound uPA proteolytic activity and surface activation of plasminogen from these cells were also reduced relative to the wild type . uPA could activate MAP kinases in the wild type and two of the cell lines with the least uPA binding , but the amount of the activated forms of the signalling molecules were reduced . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of tissue plasminogen activator ( tPA ) , urokinase ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) and inhibitor 2 ( PAI 2 ) in bile were measured by enzyme linked immunoassay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Direct evidence of the importance of stromal urokinase plasminogen activator ( uPA ) in the growth of an experimental human breast cancer using a combined uPA gene disrupted and immunodeficient xenograft model . ^^^ Several studies have indicated an interaction between tumor cells and infiltrating stromal cells regarding the urokinase plasminogen activation ( uPA ) system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect of PAI 2 on papilloma formation did not appear to involve inhibition of the secreted protease urokinase type plasminogen activator ( uPA ) : PAI 2 accumulated predominantly in cells , and PAI 2 overexpression failed to alleviate a phenotype induced by uPA secretion , as demonstrated by a double transgenic strategy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It is a specific cell surface receptor for its ligand uPA which catalyzes the formation of plasmin from plasminogen to generate the proteolytic cascade that contributes to the breakdown of extracellular matrix , a key step in cancer metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is implicated in the regulation of hepatic regeneration by activating hepatocyte growth factor ( HGF ) . ^^^ Here , we investigated its role in the hepatic regeneration after Fas mediated massive hepatocyte death employing mice deficient in either uPA or its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
After 24 hours ' incubation , tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) were measured in the medium and cell lysates using enzyme linked immunosorbent assay techniques . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cell transformation is accompanied by changes in the surrounding stroma as a result of the action of specific proteases such as the urokinase plasminogen activator ( uPA ) , which has been associated with invasive potential in many tumor types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Based on the properties of plasminogen activators in liver cell proliferation and tissue proteolysis , we explored the regulatory role of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) in liver repair . ^^^ Notably , functional cooperativity between plasminogen activators was strongly inferred from the profound reparative defect in livers of mice lacking tPA and uPA simultaneously , with persistence of centrilobular injury as far out as 35 days . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) are extracellular proteases involved in various tissue remodeling processes . ^^^ The role of plasminogen activators in skeletal muscle regeneration in vivo in wild type , uPA deficient , and tPA deficient mice is investigated here . ^^^ A similar severe regeneration deficit with persistent fibrin deposition was also reproducible in plasminogen deficient mice after injury , suggesting that fibrinolysis by uPA mediated plasminogen activation plays a fundamental role in skeletal muscle regeneration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Elevated levels of urokinase plasminogen activator 1 ( uPA ) and the insulin like growth factor 1 receptor ( IGF IR ) are associated with breast cancer recurrence and decreased survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of plasminogen to M . fermentans enhances the activation of plasminogen to plasmin by the urokinase type plasminogen activator ( uPA ) , as monitored by measuring the breakdown of chromogenic substrate S 2251 . ^^^ Enhancement was more pronounced with the low molecular weight and the single chain uPA variants , known to have low plasminogen activator activities . ^^^ Invasion was more pronounced in the presence of uPA , suggesting that the ability of the organism to invade host cells stems not only from its potential to bind plasminogen but also from the activation of plasminogen to plasmin . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition of aminopeptidase N ( AP N ) and urokinase type plasminogen activator ( uPA ) by zinc suppresses the invasion activity in human urological cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
After this , the expressions of the components of the plasminogen activator system including urokinase type and tissue type plasminogen activator ( uPA and tPA ) , urokinase receptor ( uPAR ) , and type 1 and type 2 plasminogen activator inhibitor ( PAI 1 and PAI 2 ) in strain 95D and 95C cells were determined by RT PCR and immunohistochemical staining . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a major inhibitor of urokinase type plasminogen activator ( uPA ) . ^^^ ERK phosphorylation was not induced by tissue type plasminogen activator PAI 1 complex or by uPA PAI 1 complex in the presence of antibodies that block uPA binding to uPAR . uPA PAI 1 complex induced tyrosine phosphorylation of focal adhesion kinase and Shc and sustained association of Sos with Shc , whereas uPA caused transient association of Sos with Shc . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single deficiency of uPA , tissue type PA ( tPA ) , uPA receptor , or VN , as well as combined deficiencies of uPA and tPA did not impair tumor angiogenesis , whereas lack of Plg reduced it . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytic enzymes have been identified as key molecules in tumour invasion and metastasis , and factors within the urokinase plasminogen activation ( uPA ) system have been associated with prognosis in several tumour types , including colorectal cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The aim of the study was to evaluate the prognostic significance of tumour and serum concentrations of urokinase type plasminogen activator ( uPA ) , its type 1 inhibitor ( PAI 1 ) and cathepsin D ( Cath D ) in patients with squamous cell carcinoma of the head and neck ( SCCHN ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to latent and reactive center cleaved PAI 1 and PAI 1 in complex with urokinase type plasminogen activator ( uPA ) , active PAI 1 strongly increased the fluorescence of the PAI 1 neutralizing compounds 1 anilinonaphthalene 8 sulfonic acid and 4 , 4 ' dianilino 1 , 1 ' bisnaphthyl 5 , 5 ' disulfonic acid . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urinary trypsin inhibitor ( UTI ) forms membrane complexes with UTI binding proteins ( UTI BPs ) and initiates modulation of urokinase type plasminogen activator ( uPA ) expression , which results in UTI mediated suppression of cell invasiveness . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator receptor ( uPAR ) binds pro urokinase plasminogen activator ( pro uPA ) and thereby localizes it near plasminogen , causing the generation of active uPA and plasmin on the cell surface . uPAR and uPA are overexpressed in a variety of human tumors and tumor cell lines , and expression of uPAR and uPA is highly correlated to tumor invasion and metastasis . ^^^ These uPA targeted PrAg proteins were activated selectively on the surface of uPAR expressing tumor cells in the presence of pro uPA and plasminogen . ^^^ The activation and cytotoxicity of these uPA targeted PrAg proteins were strictly dependent on the integrity of the tumor cell surface associated plasminogen activation system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purpose of this study was to compare the prognostic value of these factors with flow cytometric S phase fraction ( SPF ) , urokinase plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) in premenopausal patients with lymph node negative breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we report partial repopulation of the liver of immunodeficient urokinase type plasminogen activator ( uPA ) / recombinant activation gene 2 ( RAG 2 ) mice with normal human hepatocytes isolated from the adult liver . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Whereas HMEC proliferation was stimulated by AR , the levels of secreted urokinase type plasminogen activator ( uPA ) and type 1 plasminogen activator inhibitor ( PAi 1 ) remained unaffected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using Northern blot analysis we have measured the co expression of the matrix metalloprotease MMP 9 , plasminogen activator urokinase type ( uPA ) and its receptor ( uPAR ) mRNAs in 81 biopsies of breast carcinomas with the objective of analyzing the impact of these factors on the overall survival probability of the patients ( median follow up time : 4 years ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This protease inhibitor binding paradigm is observed at high resolution in a large set of crystal structures of trypsin , thrombin , and urokinase type plasminogen activator ( uPA ) bound with a series of small molecule inhibitors ( 2 ( 2 phenol ) indoles and 2 ( 2 phenol ) benzimidazoles ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To understand the fundamental determinants of urokinase plasminogen activator ( uPA ) driven angiogenesis in cancer we studied how inhibition of uPA activity could reduce neovascularization and consequently reduce tumor size in experimental animals . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) forms part of an important enzymatic system that degraded the extracellular matrix in process of invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Dedifferentiation of serous ovarian cancer from cystic to solid tumors is associated with increased expression of mRNA for urokinase plasminogen activator ( uPA ) , its receptor ( uPAR ) and its inhibitor ( PAI 1 ) . ^^^ We analyzed the mRNAs for urokinase plasminogen activator ( uPA ) , its receptor ( uPAR ) , and inhibitor ( PAI 1 ) in serous ovarian tumors by in situ hybridization and by densitometric scanning of Northern blots prepared from tissue extracts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plg was required for the ASR , since a deficiency of the Plg activators , urokinase ( uPA ) or tissue Plg activator ( tPA ) , did not cause a reduction in the ASR compared to their WT control . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) play an important role in the proteolytic cascade involved in the metastasis of lung and other cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) act in concert to mediate pericellular proteolysis and to stimulate intracellular signalling responsible for cell migration and proliferation . uPA is composed of three domains , a proteolytic domain ( PD ) , a kringle domain ( KD ) and a growth factor like domain ( GFD ) , the last of which mediates the interaction with uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human tissue factor pathway inhibitor 2 ( TFPI 2 ) is a Kunitz type serine protease inhibitor that inhibits plasmin , trypsin , chymotrypsin , cathepsin G and plasma kallikrein but not urokinase ( uPA ) or tissue type plasminogen activator and thrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Clinically relevant molecules whose expression or structure is altered include the plasminogen activator ( uPA ) and its inhibitor PAI 1 ( plasminogen activator inhibitor type 1 ) , the cell cycle regulator cyclin E , epidermal growth factor ( EGF ) , the apoptosis inhibitor bcl 2 , the cell adhesion molecule E cadherin , and the multifunctional protein beta catenin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This paper describes the detection of CD 44 variant sequences , urokinase type plasminogen activator ( uPA ) and uPA receptor ( uPAR ) by immunoluminescence and activity measurements . ^^^ The enhanced luminescent assay was superior to fluorescence analysis . uPA activity in intact cells was examined with the substrates plasminogen , Z Gly Gly Arg AMC and Z Lys SBzl including selective inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect of diacerein and rhein at therapeutic concentrations ( 5 20 microg / ml ) was determined by osteoblast phenotypic factors , alkaline phosphatase , osteocalcin , and cAMP ; on metabolic agents urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and insulin like growth factor 1 ( IGF 1 ) ; and on inflammatory mediators interleukin 6 ( IL 6 ) , prostaglandin E 2 ( PGE 2 ) , and cyclooxygenase 2 ( COX 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : Urinary type plasminogen activator ( uPA ) binding to uPA receptor ( uPAR ) promotes the activation of matrix metalloproteinase 9 ( MMP 9 ) , which degrades amyloid beta protein ( Abeta ) in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Met protein positive papillary carcinoma cells may produce hepatocyte growth factor ( HGF ) and may activate HGF through the urokinase type plasminogen activator ( uPA ) bound to urokinase type plasminogen activator receptor ( uPA R ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of the urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) correlates with tumour cell invasiveness and helps to determine the prognosis of prostate and other cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : Plasmin generation is controlled by the plasminogen activators ( PA ) / plasmin system , which comprises proteases ( urokinase type PA [ uPA ] and tissue type PA [ tPA ] ) and antiproteases ( PA inhibitors , PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Low density lipoprotein receptor related protein ( LRP ) mediates internalization of urokinase : plasminogen activator inhibitor complexes ( uPA : PAI 1 ) and the urokinase receptor ( uPAR ) . ^^^ Here we investigated whether direct interaction between uPAR , a glycosyl phosphatidylinositol anchored protein , and LRP , a transmembrane receptor , is required for clearance of uPA : PAI 1 , regeneration of unoccupied uPAR , activation of plasminogen , and the ability of HT 1080 cells to invade extracellular matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cancer tissues from 71 colorectal cancer patients were assayed quantitatively for antigen levels of urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , and plasminogen activator inhibitor 1 and 2 ( PAI 1 , PAI 2 ) , and were also assayed immunohistochemically for expression of VEGF protein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase plasminogen activator ( uPA ) is thought to play a central role in tumor metastasis and angiogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : Nave mice intracorneally infected with P . aeruginosa showed a temporally enhanced expression of tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) , and plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) , over a several day holding period . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator system ( uPA ) has been demonstrated to be required for the movement of cells through a matrix . ^^^ These observations have been extended to the migration of endothelial cells during the process of angiogenesis , and recent data suggest that the uPA system is central to this process . uPA is a serine protease that is capable of initiating an extracellular cascade of proteolysis that involves the activation of plasminogen and matrix metalloproteases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To elucidate the role of matrix metalloproteinases ( MMP ) in carcinogenesis , the expression of collagenases of types 1 ( MMP 1 ) and 4 ( MMP 2 and MMP 9 ) as well as the behaviour of urokinase like plasminogen activator ( uPA ) and of tissue MMP inhibitors ( TIMP ) in immortalized ( IF ) and transformed ( TF ) fibroblasts were investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : The endogenous expressions of tissue type plasminogen activator ( tPA ) , urokinase ( uPA ) , and PA inhibitor 1 ( PAI 1 ) were quantified in 10 microm frozen sections from ischemic and matched nonischemic basal ganglia and in the plasma of 34 male healthy nonhuman primates before and after middle cerebral artery occlusion ( MCA : O ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of urokinase type plasminogen activator receptor ( uPAR ) and plasminogen activator inhibitor 1 ( PAI 1 ) , critical components of the urokinase plasminogen activator ( uPA ) system , was lower and delayed in IL 6 / livers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we purified an HIV 1 suppressing activity from the culture supernatant of an immortalized CD8+ T cell clone , derived from an HIV 1 infected long term nonprogressor , and identified this activity as the amino terminal fragment ( ATF ) of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A significant body of in vitro and in vivo data has established the urokinase plasminogen activator ( uPA ) system as a promising target for cancer drug development . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of the urokinase type plasminogen activator ( uPA ) and its receptor ( CD 87 ) in lipodermatosclerosis . ^^^ METHODS : The expression and the functional state of the urokinase type plasminogen activator ( uPA ) , the tissue type plasminogen activator ( tPA ) , the urokinase receptor ( CD 87 ) , the plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) were assayed using reverse transcription polymerase chain reaction , Western blot , fibrin zymography and immunohistochemistry analyses in tissue samples of lipodermatosclerosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mice lacking tPA , uPA , or plasminogen genes showed delayed functional recovery after sciatic nerve crush . ^^^ These proteases include the plasminogen activators ( PAs ) , tissue PA ( tPA ) and urokinase PA ( uPA ) , and their substrate , plasminogen . ^^^ To further assess the role of the PA system during peripheral nerve regeneration , PA dependent activity as well as recovery of sensory and motor function in the injured hindlimb were assessed in wild type , tPA , uPA , and plasminogen knock out mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) in concert with other proteolytic enzymes plays a critical role in cartilage degradation during osteoarthritis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Stimulation of proliferative activity by urokinase type plasminogen activator ( uPA ) has been demonstrated in vitro for cultured primary and carcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Caco 2 cells were found to express HGF receptor but not HGF and to secrete several proteases , namely matrix metalloproteinase 1 ( MMP 1 ) , MMP 2 , possibly MMP 9 and urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Focusing of urokinase type plasminogen activator ( uPA ) to the cell surface via binding to its specific receptor ( uPAR , CD 87 ) is critical for tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : Urokinase type plasminogen activator ( uPA ) and its inhibitor , plasminogen activator inhibitor ( PAI ) 1 , have been shown to be related to poor prognosis in a variety of malignant solid tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As measured by Northern blot hybridization and zymography , TGF beta treatment of PANC 1 and IMIM PC 1 cells resulted in strong up regulation of expression and activity of both matrix metalloproteinase 2 ( MMP 2 ) and the urokinase plasminogen activator ( uPA ) system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
On the surface of certain cells , uPARAP forms a ternary complex with the pro form of the urokinase type plasminogen activator ( uPA ) and its primary receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
LRP is a scavenging receptor for ligands from extracellular fluids , e . g . alpha ( 2 ) macroglobulin ( alpha ( 2 ) M ) proteinase complexes , lipoprotein containing particles and serine proteinase inhibitor complexes , like the complex between urokinase type plasminogen activator ( uPA ) and the plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In a unicenter study , we have found that levels of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) in the primary tumor are predictive of disease recurrence . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We showed previously that pemphigus IgG enhanced both the activity of urokinase plasminogen activator ( uPA ) in cultured cells and the expression of its receptor ( uPAR ) on uPA binding keratinocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Upon hatching , the extracellular matrix degrading proteinases urokinase plasminogen activator ( uPA ) and matrix metalloproteinase 9 ( MMP 9 ) are thought to promote blastocyst invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , expression and selected activities of urokinase type plasminogen activator ( uPA ) , matrix metalloproteinases ( MMPs ) , their inhibitors ( plasminogen activator inhibitor 1 ( PAI 1 ) and tissue inhibitors of metalloproteinases ( TIMPs ) ) were examined in bovine oviducts by RT PCR , ribonuclease protection assay and activity assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MCs reacted with monoclonal antibodies to tryptase , chymase , and c kit / CD117 and stained positively for tissue type plasminogen activator ( tPA ) and urokinase receptor ( uPAR / CD87 ) but did not express detectable urokinase ( uPA ) or plasminogen activator inhibitors ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PSK also suppressed the expression of urokinase plasminogen activator ( uPA ) and uPA receptor but did not change plasminogen activator inhibitor 1 ( PAI 1 ) expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Periadventitial application of the urokinase plasminogen activator ( uPA ) in pluronic gel to an injured artery stimulated the neointima and neoadventitia formation as well as cell migration and proliferation in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One of the phenotypic changes induced in migrating cells is the increased expression of urokinase plasminogen activator ( uPA ) and of its specific receptor uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is believed to play a key role in tissue degradation and cell migration under various normal and pathological conditions , including cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It not only functions as an inhibitor of the protease uPA ( urokinase type plasminogen activator ) , but also plays an important role in signal transduction , cell adherence , and cell migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As an example , matrix degrading protease systems are discussed , in particular the urokinase plasminogen activator ( uPA ) system in which inhibition of binding of uPA to its cellular receptor is an attractive target . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytic enzymes , like urokinase ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) , are involved in remodelling tissues during invasion and metastasis of tumor cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Therefore , the expression of the matrix metalloproteinase ( MMP ) 2 and MMP 9 and of the serine protease urokinase type plasminogen activator ( uPA ) and its inhibitor plasminogen activator inhibitor type 1 ( PAI 1 ) in the progression of ovarian cancer was investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : Recent studies suggest that HER 2 / neu specifically promotes the invasive capacity of tumor cells by up regulating secretion of the proteolytic enzyme , urokinase type plasminogen activator ( uPA ) , or its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , in colon and gastric cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A key molecule in regulating plasminogen mediated extracellular proteolysis is the urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
AIMS : Weak staining for urokinase plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , or plasminogen activator inhibitor 1 ( PAI 1 ) confined to crescents has been described in a few cases of severe crescentic glomerulonephritis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The urokinase plasminogen activator ( uPA ) system has been implicated in cellular invasiveness of tumor cells and immune cells . ^^^ MATERIALS AND METHODS : Western blot analysis , RTPCR , casein / plasminogen zymography , and fluorescence microscopy were employed to detect uPA and uPAR on NK cells . ^^^ RESULTS : NK cell uPA appeared at its characteristic molecular weights , is enzymatically active in casein / plasminogen zymography , and is recognized by monoclonal antibodies . uPAR was detected by RTPCR and fluorescence microscopy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The interaction between urokinase plasminogen activator ( uPA ) and its cellular receptor ( uPAR ) is a key event in cell surface associated plasminogen activation , relevant for cell migration and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We localized the epitopes for several murine mAbs to human urokinase type plasminogen activator ( uPA ) by Ala scanning mutagenesis and related the localization to the effects of the mAbs on the molecular interactions of uPA . ^^^ Several antibodies against the serine proteinase domain ( SPD ) were found to have overlapping epitopes composed of variable combinations of Arg 178 , Arg 179 , His 180 , Arg 181 , Tyr 209 , Lys 211 , and Asp 214 in the so called 37 loop and 60 loop , located near the active site and taking part in the binding of uPA to plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^ Besides inhibiting uPA catalysed plasminogen activation , all antibodies to SPD strongly delayed the binding of uPA to PAI 1 , decreasing the second order rate constant 15 to 6500 fold . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : A balance between urokinase type plasminogen activator ( uPA ) and its main inhibitor type 1 ( PAI 1 ) appears to be important for cancer invasive behavior . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enzyme immunoassay ( ELISA ) was used to estimate the levels of plasminogen activators of urokinase ( uPA ) and tissue ( tPA ) types and one of their inhibitors ( PAI 1 ) in the cytosolic fraction of the thyroid in 129 patients with malignant and benign tumors and various non cancer diseases of the gland . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HGF further induced expression of the Ets 1 and urokinase type plasminogen activator ( uPA ) genes specifically in E1AF transfected cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its cell surface receptor ( uPAR ) regulate cellular functions linked to adhesion and migration and contribute to pericellular proteolysis in tissue remodelling processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Analysis of the proteolytic plasminogen activator system revealed that endostatin modulates the distribution of soluble and cell surface associated urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor , type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This activity was fully inhibited by inhibitors of urokinase type plasminogen activator ( uPA ) , including PA inhibitor 1 , an anti uPA mAb , and amiloride . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Laminar flow ( 1 ml / min ) induced a reorganization of the actin cytoskeleton and significantly inhibited the expression of plasminogen activators [ tissue type ( tPA ) activity : 25 % of control cells ; tPA mRNA : 70 % of control cells ; urokinase ( uPA ) mRNA : 56 % of control LLC PK ( 1 ) cells ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Quality control preparations for the immunocytochemical assessment of urokinase type plasminogen activator ( uPA ) were developed using different combinations of cultured cell lines ( BLM and IF 6 ) , each expressing immunochemically well defined ( by enzyme linked immunosorbent assay [ ELISA ] ) amounts of the respective component . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two renal cell carcinoma cell lines ( 49RC 43STR and 75RC 2STR ) were characterized by detection of the cell surface proteins : CD 44 ( var ) , intercellular adhesion molecule 1 ( ICAM 1 ) , urokinase type plasminogen activator ( uPA ) and its receptor and aminopeptidase N ( APN ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To establish the necessary and sufficient components of the PA system [ PAI 1 , urokinase type PA ( uPA ) , or tissue type PA ( tPA ) , and plasminogen ( Plg ) ] for angiogenesis , we examined angiogenic competence of vascular explant cultures obtained from mice deficient in PAI 1 , tPA , uPA , and Plg . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type ( tPA ) and urokinase type ( uPA ) plasminogen activator enzymic activities were detectable in cultured saphenous vein segments , and were concentrated in focal zones . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study examined the role of oxygen , integrins and the urokinase type plasminogen activator ( uPA ) system on the adhesion of trophoblast and breast carcinoma cells to the ECM molecules vitronectin and fibronectin . ^^^ Furthermore , adhesion to vitronectin and fibronectin was reduced by compounds that interfere with integrin function , such as EDTA , anti integrin antibodies , or by antibodies that interfere with the binding of pro uPA to uPAR , soluble uPAR , soluble vitronectin , phosphatidylinositol specific phospholipase C , as well as plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PAI 1 ( plasminogen activator inhibitor 1 ) binds the urokinase type plasminogen activator ( uPA ) and causes its degradation via its receptor uPAR and low density lipoprotein receptor related protein ( LRP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) has been implicated in the metastatic potential of ovarian carcinomas of surface epithelial origin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Highly significant quantitative increases in urokinase PA ( uPA ) , urokinase receptor ( uPAR ) and plasminogen activator inhibitor 1 were detected in acute multiple sclerosis lesions ( P < 0 . 0001 ) and in uPAR in normal appearing white matter ( P < 0 . 0001 ) compared with control tissue . ^^^ In contrast to uPA , the concentration and activity of tissue PA ( tPA ) , the most abundant plasminogen activator in normal control brain , were reduced in multiple sclerosis specimens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we characterized the production of fibrinolytic factors including tissue type plasminogen activator ( tPA ) , urokinase type PA ( uPA ) , and PAI 1 in the differentiation of preadipocytes , and examined the hormonal regulation of these fibrinolytic factors in mature adipocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The high affinity interaction between urokinase type plasminogen activator ( uPA ) and its glycolipid anchored receptor ( uPAR ) plays an important role in pericellular plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , transplantation of woodchuck hepatocytes into the liver of urokinase type plasminogen activator ( uPA ) / recombination activation gene 2 ( RAG 2 ) mice , a model of liver regeneration , demonstrated that cryopreserved cells retained the ability to divide and to extensively repopulate a xenogenic liver . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) binds to the uPA receptor ( uPAR ) and activates the Ras extracellular signal regulated kinase ( ERK ) signaling pathway in many different cell types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The generation of urokinase plasminogen activator ( uPA ) by tumours is an important pathway for neoplastic cell invasion and metastasis . ^^^ Indeed in several tumour types , elevated levels of uPA , its receptor ( uPAR ) or its inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) is associated with a poorer prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cyclo 19 , 31 [ D Cys 19 ] uPA 19 31 is a potent competitive antagonist of the interaction of urokinase type plasminogen activator with its receptor ( CD 87 ) . ^^^ Urokinase type plasminogen activator ( uPA ) represents a central molecule in pericellular proteolysis and is implicated in a variety of physiological and pathophysiological processes such as tissue remodelling , wound healing , tumor invasion , and metastasis . uPA binds with high affinity to a specific cell surface receptor , uPAR ( CD 87 ) , via a well defined sequence within the N terminal region of uPA ( uPA 19 31 ) . ^^^ Cyclo 19 , 31 [ D Cys 19 ] uPA 19 31 not only blocks binding of uPA to uPAR but is also capable of efficiently displacing uPAR bound uPA from the cell surface and to inhibit uPA mediated , tumor cell associated plasminogen activation and fibrin degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Several studies have indicated that the serine protease urokinase plasminogen activator ( uPA ) is an important factor in host defense against pulmonary pathogens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The components of the plasminogen activator system were measured , i . e . tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A panel of eight molecular markers of metastatic potential was chosen for immunohistochemical analysis of the tumor : p 53 , erbB 2 , angiogenesis factor 8 , EphA 2 , E cadherin , urokinase plasminogen activator ( UPA ) , UPA receptor , and plasminogen activator inhibitor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Chem . 2001 , 44 , 2753 2771 ) has been optimized through minor structural changes on the S 1 binding group to afford remarkably selective and potent inhibitors of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have demonstrated that certain aggressive breast cancer cell lines , which have highly activated endogenous urokinase type plasminogen activator ( uPA ) uPA receptor ( uPAR ) systems , do not express high levels of cell surface K 8 . ^^^ The membrane macromolecule that is responsible for plasminogen binding and for supporting activation of plasminogen by uPA on the surfaces of these cell types remains to be determined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) promoter contains an NF kappaB binding site , and uPA expression in MDA MB 231 cells is induced by the constitutively active NF kappaB . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To test this hypothesis , primary human invasive colonocytes were isolated from fresh surgical specimens and treated with 0 . 01 mol / L acetate , propionate or butyrate ; cell invasion , cell adhesion , F actin polymerization , urokinase plasminogen activator ( uPA ) , tissue inhibitor matrix metalloproteinase ( TIMP ) 1 , TIMP 2 and mutant p 53 , Bcl 2 , Bax , p 21 and proliferating cell nuclear antigen ( PCNA ) protein expression levels were examined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Antigen driven lymphocyte recruitment to the lung is diminished in the absence of urokinase type plasminogen activator ( uPA ) receptor , but is independent of uPA . ^^^ The requirement for urokinase plasminogen activator ( uPA ) and uPA receptor ( uPAR ) in T lymphocyte migration is unknown . uPA ( / ) mice have fewer pulmonary lymphocytes in response to certain infections , but its unknown whether this is due to diminished recruitment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) regulates a proteolytic cascade that facilitates cancer invasion through degradation of the extracellular matrix , and high levels of uPA in human breast cancer tissue correlate with poor prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study we examined the relation of uPA ( urokinase plasminogen activator ) , uPAR ( uPA receptor ) and PAI 1 ( plasminogen activator inhibitor type 1 ) to the biological growth behavior of esophageal cancer , as well as their influence on survival in esophageal cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There is abundant evidence that the plasminogen activator ( PA ) system with its key components uPA ( urokinase type plasminogen activator ) , its cell surface receptor uPA R ( CD 87 ) and its inhibitor PAI 1 plays a key role in tumour invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The matrix metalloprotease ( MMP ) family of enzymes and the urokinase plasminogen activator ( uPA ) pathway have both been implicated in tumor invasion and metastasis and in poor prognosis of cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There is ample information on the clinical role of biologic factors in female breast cancer : urokinase type plasminogen activator ( uPA ) , its receptor uPAR , its inhibitors PAI 1 and PAI 2 , cathepsin D and pS 2 protein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have recently reported that the urokinase type plasminogen activator ( uPA ) up regulates the cell surface expression of its own receptor ( uPAR ) in several cell types , independently of its enzymatic activity . uPA has no effect on kidney 293 cells which do not express uPAR and then can not bind uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator ( uPA ) and uPA receptor ( uPAR ) plays a role in destruction of joint tissue in rapidly destructive coxarthropathy ( RDC ) ] . ^^^ To examine the relationship between the angiogenesis and bone destruction , expression of urokinase type plasminogen activator ( uPA ) and uPA receptor ( uPAR ) , which are necessary for angiogenesis was histochemically investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) has been implicated in the healing responses of injured arteries , but the importance of its various properties that influence smooth muscle cell ( SMC ) proliferation and migration in vivo is unclear . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In one class there is a serine at position 190 at the S 1 site , as in urokinase type plasminogen activator ( urokinase or uPA ) and factor VIIa , and in the other there is an alanine at 190 , as in tissue type plasminogen activator ( tPA ) and factor Xa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombin / antithrombin 3 complex ( TAT ) , soluble fibrin ( SF ) , total fibrinogen and fibrin degradation products ( TDP ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , urokinase type plasminogen activator ( uPA ) and uPA receptor ( uPAR ) were measured preoperatively , starting anesthesia , during surgery and postoperatively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
AIM : To determine the activity of glutathione ( GSH ) and concentrations of glutathione S transferases ( GST ) , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) , and to evaluate their diagnostic and prognostic value and possible correlation with clinical and histopathological prognostic factors for ovarian carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They are classified into two distinct subtypes , tissue plasminogen activating factor and urokinase plasminogen activating factor ( tPA and uPA , respectively ) , which are both expressed in brain areas thought to be important in learning and memory . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of tPA and uPA activity , and is expressed in corresponding brain areas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of tissue factor ( TF ) , the primary initiator of hemostasis ; urokinase type plasminogen activator ( uPA ) ; tissue type plasminogen activator ( tPA ) ; plasminogen activator inhibitor 1 ( PAI 1 ) as well as the potent matrix metalloprotease , MMP 3 was assessed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recombinant maspin has been shown to specifically inhibit cell surface associated urokinase type plasminogen activator ( uPA ) and fibrinogen bound tissue type plasminogen activator . ^^^ A combination of enzymatic and molecular analyses revealed that maspin inhibits the cell surface mediated plasminogen activation by forming an SDS resistant complex with cell surface bound uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Others and the authors of this study recently identified cathepsin D , stromelysin 3 , and urokinase plasminogen activator ( uPA ) expression by reactive stromal cells as significant factors of poor prognosis in breast carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Selective urokinase type plasminogen activator ( uPA ) inhibitors . ^^^ The identification of 2 pyridinylguanidines ( e . g . , 27 and 28 ) as selective inhibitors of urokinase type plasminogen activator ( uPA ) is described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Selective urokinase type plasminogen activator ( uPA ) inhibitors . ^^^ Based on previous modeling predictions , a series of ( 3 substituted 5 chloro 2 pyridinyl ) guanidines have been designed with good potency and selectivity for urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of urokinase type plasminogen activator ( uPA ) to its receptor ( uPAR ) in various cell types has been proposed as an important feature of many cellular processes requiring extracellular proteolysis , cell adhesion , motility , and invasion . uPAR attaches to the cell surface with a glycosylphophatidylinositol ( GPI ) anchor , and serves to localize and accelerate the proteolysis cascade . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Components investigated were the fibrinolytic stimulator tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( uPA ) and the antagonist . plasminogen activator inhibitor type one ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The removal of hydrocortisone increased urokinase plasminogen activator ( uPA ) activity within 24 48 h and tPA activity within 48 72 h , and converted the cells to a more elongated and fibroblastic phenotype . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In our pilot study , we analyzed the clinical relevance of YB 1 expression in breast cancer ( n = 83 ) after a median follow up of 61 months and compared it with tumor biologic factors already used for clinical risk group discrimination , i . e . , HER 2 , urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
On the other hand , high tumor tissue concentrations of urokinase plasminogen activator ( uPA ) , uPA receptor ( R ) and PAI 1 have more consistently been associated with poor histologic differentiation and poor prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The invasiveness of these tumors requires proteases , some of which as urokinase type plasminogen activator ( uPA ) , gelatinase B and matrilysin are regulated through AP 1 dependent transcriptional mechanisms . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human ovarian cancer cell line , HRA , was treated with phorbol ester ( PMA ) in order to evaluate the effect on expression of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the expression and clinical significance of urokinase type plasminogen activator ( uPA ) in human gliomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expression and clinical significance of urokinase type plasminogen activator ( uPA ) in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We previously found that bikunin ( bik ) , a Kunitz type protease inhibitor , suppresses phorbol ester ( PMA ) stimulated expression of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) ( / ) mice can not mount protective host defenses during infection with the opportunistic yeast Cryptococcus neoformans ( 52D ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) system is responsible for invasion through uPA enzymatic activity and for migration through the binding of uPA to the uPA receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
DEX and IL 4 could also suppress the expression of urokinase type plasminogen activator ( uPA ) to prevent the conversion from the proenzyme form of MMP 9 to its active form . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition to inhibiting serine proteases , mainly tissue type ( tPA ) and urokinase type ( uPA ) plasminogen activators , PAI 1 interacts with different components of the extracellular matrix , i . e . fibrin , heparin ( Hep ) and vitronectin ( Vn ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , we used immunohistochemistry and in situ hybridization techniques to determine the localization of urokinase type ( u PA ) and tissue type ( t PA ) plasminogen activators , type 1 plasminogen activator inhibitor ( PAI 1 ) and membrane receptor for u PA ( uPA R ) antigen and their sites of synthesis in renal thrombotic microangiopathy ( N = 10 ) as compared to acute tubular necrosis ( N = 5 ) and normal human kidneys ( N = 7 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) system is a dynamic complex in which the membrane receptor uPAR binds uPA that binds the plasminogen activator inhibitor ( PAI ) 1 localized in the extracellular matrix , resulting in endocytosis of the whole complex by the low density lipoprotein receptor related protein ( LRP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase type plasminogen activator ( uPA ) and its inhibitor ( PAI 1 ) play essential roles in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasma membrane urokinase plasminogen activator receptor ( uPAR ) localizes and enhances activation of pro uPA . ^^^ Active uPA , in turn , promotes increased degradation of the extracellular matrix ( ECM ) by activation of plasminogen . uPAR binds to ECM molecules and integrins , which can affect cellular adhesion , signal transduction , and gene regulation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Antibodies to both uPA and uPA receptor ( uPAR ) were shown to significantly inhibit cell invasion , as did the uPA inhibitors ( plasminogen activator inhibitor 1 [ PAI 1 ] , p aminobenzamidine [ PABN ] , aprotinin , and amiloride ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Within 1 h of treatment , cultures accumulated an extracellular activity capable of cleaving a substrate for urokinase type plasminogen activator ( uPA ) and tissue plasminogen activator ( tPA ) . ^^^ This activity was inhibited by plasminogen activator inhibitor 1 or antibodies to uPA but not tPA . ^^^ Subsequently , extracellular cathepsin B initiates a proteolytic cascade involving uPA , plasminogen , and plasmin that activates serum derived latent TGF beta . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the plasmin dependent pathway , the expression of plasminogen activator inhibitor ( PAI 1 ) mRNA was continuously increased , while the expression of urokinase type plasminogen activator ( uPA ) mRNA was not increased . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We now show that FPRL1 / LXA4R , a G protein coupled receptor for a number of polypeptides and for the endogenous lipoxin A 4 ( LXA 4 ) , is the link between urokinase type plasminogen activator ( uPA ) and migration as it directly interacts with an activated , soluble , cleaved form of uPA receptor ( uPAR ) ( D2D3 ( 88 274 ) ) to induce chemotaxis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Electrophoresis ( 8 . 5 % SDS PAGE ) revealed that fibrin formed in the presence of ACP demonstrated characteristic gamma gamma dimers ( 90 kDa ) and beta monomers ( 55 kDa ) , but resisted spontaneous fibrinolysis ( 72 h , 37 degrees C ) or degradation by plasminogen activators ( uPA , tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression levels and the prognostic impact of urokinase type plasminogen activator ( uPA ) and cathepsin D ( CD ) were evaluated in patients with locally advanced laryngeal squamous cell carcinoma ( LSCC ) . uPA and CD protein levels were determined by immunoluminometric or immunoenzymatic assays in the cytosol of paired sets of tumor tissues and corresponding adjacent normal mucosa ( NLM ) from 57 patients with stage III / IV LSCC and were correlated with a number of clinicobiological parameters of this tumor including anatomical site , tumor grade , nodal status , clinical stage , DNA ploidy , proliferation rate , and patient outcome . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By using a cDNA subtraction hybridization method , we identified that NF IL3A and urokinase type plasminogen activator ( uPA ) genes were up regulated by CT in porcine renal cell line LLC PK 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When negative PAI 1 conupared with positive urokinase type plasminogen activator ( uPA ) and positive uPA receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of alphavbeta 6 in OVCA 429 and OVCA 433 was far higher than alphavbeta 3 and alphavbeta 5 integrin and correlated with high p42 / p44 mitogen activated protein kinase ( MAPK ) activity and high secretion of high molecular weight urokinase plasminogen activator ( HMW uPA ) , pro metalloproteinase 2 and 9 ( pro MMP 9 and pro MMP 2 ) . ^^^ Plasminogen dependent [ 3H ] collagen 4 degradation was inhibited by inhibitor of uPA ( amiloride ) and MMP ( phenanthroline ) and by antibodies against uPA or MMP 9 or alphavbeta 6 integrin , indicating the involvement of alphavbeta 6 integrin , uPA and MMP 9 in the process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : This study was undertaken to evaluate the significance of cyclooxygenase 2 ( COX 2 ) activity on urokinase plasminogen activator ( uPA ) and matrix metalloproteinases ( MMPs ) 1 and 9 induction in cornea following platelet activating factor ( PAF ) treatment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We therefore analyzed the migrating and invasive potential of human HL 60 and NB 4 cell lines , representative of acute myelogenous leukemia , their ability to express matrix metalloproteases ( MMPs ) , tissue inhibitors of metalloproteases ( TIMPs ) and urokinase plasminogen activator ( uPA ) in response to differentiating agents . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : A strong prognostic impact of urokinase type plasminogen activator ( uPA ) and its inhibitor and plasminogen activator inhibitor type 1 ( PAI 1 ) as individual factors is well established in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human endothelial cell functions involved in angiogenesis , namely proliferation , chemotaxis , morphogenesis , and secretion of matrix metalloproteinase 2 ( MMP 2 ) , MMP 9 , and urokinase type plasminogen activator ( uPA ) were studied in vitro upon exposure to docetaxel and paclitaxel , whereas their effect on angiogenesis was studied in vivo by using the chick embryo chorioallantoic membrane ( CAM ) model . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The significance of urokinase type plasminogen activator ( uPA ) expression in gastric cancer development was tested by using a human uPA cDNA transfection approach and an in vivo severe combined immunodeficient ( SCID ) mouse model . ^^^ The AGS gastric cancer cell line , which has urokinase type plasminogen activator receptor ( uPAR ) but lacks uPA , was transfected with a plasmid containing human uPA cDNA and injected into the backs of SCID mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) uPA receptor ( uPAR ) and epidermal growth factor ( EGF ) EGF receptor ( EGFR ) expression is highly correlated with breast cancer metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Levels of tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor ( PAI ) type 1 ( PAI 1 ) and type 2 ( PAI 2 ) , and tPA / PAI complex in tissue extracts were determinated by commercially available enzyme linked immunosorbent assay kits . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
High and moderate expression of alpha ( 5 ) beta 6 integrin correlated with increased plasminogen dependent degradation of extracellular matrix which could be inhibited by inhibitors of plasmin , uPA and MMPs or by monoclonal antibody against uPA , MMP 9 or alpha ( 5 ) beta 6 integrin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and matrix metalloproteinase 9 ( MMP 9 ) expression and activity during early embryo development in the cow . ^^^ In particular , urokinase type plasminogen activator ( uPA ) and matrix metalloproteinase 9 ( MMP 9 ) are expressed in peri implantation mouse , sheep , and pig embryos and are implicated in the implantation process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is implicated in the control of cell adhesion and invasion , and is regarded as a strong prognostic marker in colorectal cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This construct exploits : ( a ) the overexpression of the cell surface receptor bound urokinase plasminogen activator ( uPA ) in the metastatic spread of breast cancer cells ; ( b ) the binding and inhibition of receptor bound uPA by PAI 2 ; and ( c ) the high cytotoxicity of alpha radiation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both compounds significantly decreased urokinase plasminogen activator ( uPA ) and matrix metalloproteinases 9 ( MMP 9 ) concentrations in conditioned media . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and its receptor uPAR play a major role in immune cell mediated , including natural killer ( NK ) cell mediated , degradation of extracellular matrices . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the role of uPAR and urokinase plasminogen activator ( uPA ) during pneumonia caused by a beta ( 2 ) integrin independent respiratory pathogen , Streptococcus pneumoniae . uPAR deficient ( uPAR ( / ) ) , uPA deficient ( uPA ( / ) ) , and wild type ( Wt ) mice were intranasally inoculated with 10 ( 5 ) CFU S . pneumoniae . uPAR ( / ) mice showed reduced granulocyte accumulation in alveoli and lungs when compared with Wt mice , which was associated with more S . pneumoniae CFU in lungs , enhanced dissemination of the infection , and a reduced survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( tPA ) and urokinase ( uPA ) are targets of plasminogen activator inhibitor 1 ( PAI 1 ) inhibition . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) and the uPA receptor ( uPAR ) are key components in the plasminogen activation system , serving to promote specific events of extracellular matrix degradation in connection with tissue remodeling and cancer invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
After induction of a focal ischemic stroke in the mouse by occlusion of the middle cerebral artery , we found that microglial cells accumulated in the marginal zone of the infarct are the most important source for both plasminogen activators , tPA and uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine proteinase inhibitor plasminogen activator inhibitor type 1 ( PAI 1 ) is the primary physiological inhibitor of the tissue type and the urokinase type plasminogen activator ( tPA and uPA , respectively ) and as such an important regulator of proteolytic events taking place in the circulation and in the extracellular matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) expressed by monocytes is a potent chemotactic factor for VSMC and might serve for the acceleration of vascular remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Active plasmin is generated from proteolytic cleavage of the zymogen plasminogen ( Plg ) by urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have explored intracellular pathways involved in the urokinase type plasminogen activator ( urokinase or uPA ) stimulated migration of human airway smooth muscle cells ( hAWSMC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activation system , including the serine protease uPA ( urokinase type plasminogen activator ) and its cell surface receptor ( uPAR , CD 87 ) , are important key molecules in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Its antiinvasive action is due to an upregulation of tissue inhibitor of matrix metalloprotease 1 ( TIMP 1 ) and plasminogen activator inhibitor ( PAI 1 ) and a downregulation of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of the present study was to determine the level of urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and plasminogen activator inhibitor type 2 ( PAI 2 ) in normal and malignant tissues of corpus uteri and to evaluate the possible correlation with clinical and histopathological prognostic factors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
AIMS : To establish that cells from the murine mammary carcinoma cell line , EMT 6 , express type 1 insulin like growth factor receptor ( IGF IR ) , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urinary plasminogen activator or urokinase ( uPA ) , produced in abundance by many malignancies , plays a key role in tumor cell invasion and metastasis . uPA is localized within the malignant cell milieu via its cell surface receptor [ uPA receptor ( uPAR ) ] , which is expressed by tumor and tumor associated cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Anti urokinase type plasminogen activator ( uPA ) and anti uPAR antibodies reduced the migration and invasion activities of R 1 and R 2 cells to baseline levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Emerging data suggest that urokinase type plasminogen activator ( UPA ) , beyond its role in pericellular proteolysis , may also act as a mitogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the expression of oestrogen ( ER ) and progesterone receptor ( PgR ) , Ki 67 , p 53 , bcl 2 proteins and the proteolytic enzymes cathepsin D ( CD ) , urokinase plasminogen activator ( uPA ) and its receptor ( uPA R ) in primary carcinomas of the breast from indigenous Tanzanian female patients by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibitors of urokinase type plasminogen activator ( uPA ) were selected in vitro from two ecotin phage display libraries to study the effect on binding of amino acid substitutions at critical positions 108 , 110 , 112 , and 113 within the 100s loop ( RNKL , respectively , in wild type ecotin ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) receptor ( uPAR ) is expressed on the surface of glioblastoma and some other tumor cells and endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The components of the PPS include the urokinase plasminogen activator ( uPA ) , its cell surface receptor urokinase plasminogen activator receptor ( uPAR ) , and its naturally occurring inhibitors , plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Pericellular proteolysis initiated by receptor bound urokinase type plasminogen activator ( uPA ) is considered important for directed migration of granulocytes to inflammatory sites . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study examined the effect of the preovulatory gonadotropin surge on the temporal and spatial regulation of tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , and uPA receptor ( uPAR ) mRNA expression and tPA , uPA , and plasmin activity in bovine preovulatory follicles and new corpora lutea collected at approximately 0 , 6 , 12 , 18 , 24 , and 48 h after a GnRH induced gonadotropin surge . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Invasion and metastasis of solid tumors require tumor biologic factors that promote the dissolution of the surrounding tumor matrix and the basement membranes , and the serine protease urokinase type plasminogen activator ( uPA ) and its inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) , play major roles in these invasive processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We chose urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and Cathepsin D as invasive markers , and Ki 67 and C erbB 2 oncoproteins as proliferative factors for our study . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study aimed to assess the role of fibrinolysis in adhesion formation using mice deficient in either of the plasminogen activator proteases , tissue type plasminogen activator ( tPA ) or urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of a glycolipid anchored receptor for urokinase type plasminogen activator ( uPA ) targets this system to the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a serine protease that is causally involved in cancer progression , especially invasion and metastasis . ^^^ Paradoxically , high levels of plasminogen activator inhibitor 1 ( PAI 1 ) , an endogenous inhibitor of uPA , also predict for aggressive disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect of recombinant PrP , either containing copper ( holo PrP ) or devoid of it ( apo PrP ) , on plasminogen activation by both uPA and tPA was determined . ^^^ PrP had no effect on plasminogen activation by uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Adhesion of monocytes to the extracellular matrix is mediated by a direct high affinity interaction between cell surface urokinase type plasminogen activator ( uPA ) receptor ( uPAR ) and the extracellular matrix protein vitronectin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We then sought to identify ER 81 target genes , and the urokinase plasminogen activator ( uPA ) and the stromelysin 1 , two enzymes involved in extracellular matrix degradation , were found to be transcriptionally upregulated in lactating mammary glands over expressing ER 81 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Angiostatin is generated from plasminogen by urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators in the presence of free sulphydryl donors . ^^^ We found that human thyroid cells in culture secrete plasminogen activators ( both tPA and uPA ) as well as matrix metalloproteinase 2 into the medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In cranial sutural samples derived from five children with premature cranial suture fusion we have performed immunostaining for the urokinase plasminogen activator ( uPA ) and urokinase receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since anti invasive effects of TGF beta in the normal EVT cells were shown to be mediated in part by plasminogen activator inhibitor 1 ( PAI 1 ) and urokinase type plasminogen activator ( uPA ) , we compared the expression of PAI 1 and uPA in the normal EVT , JAR , and JAR smad3 / c cells in the presence or absence of TGF beta 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Elevated levels of soluble urokinase type plasminogen activator ( uPA ) receptor , CD87 / u PAR , predict survival in individuals infected with HIV 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Accumulated clinical and experimental evidence indicates that the urokinase type plasminogen activator ( uPA ) and its regulators are causatively involved in the metastatic phenotype of many types of cancers . ^^^ A current consensus suggests that the uPA system promotes PC metastasis by mediating pericellular plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Northern blot analysis demonstrated that genes encoding urokinase type plasminogen activator ( uPA ) , urokinase type plasminogen activator receptor ( uPAR ) , plasminogen activator inhibitor 1 ( PAI 1 ) , tissue inhibitor of metalloproteinases ( TIMP 1 ) and c myc were up regulated in response to hyaluronan . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The development of potent and selective urokinase type plasminogen activator ( uPA ) inhibitors based on the lead molecule 2 ( 2 hydroxy 3 ethoxyphenyl ) 1H benzimidazole 5 carboxamidine ( 3a ) is described . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The structure based design of potent and selective urokinase type plasminogen activator ( uPA ) inhibitors with 4 aminoarylamidine or 4 aminoarylguanidine S 1 binding groups , is described . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) interacts with its receptor ( uPAR ) to promote local proteolysis as well as cellular proliferation and migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) mRNA was determined in 194 subjects with newly detected bladder neoplasms , selected from a larger population based series . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is suggested to exert its proliferatory , migratory and invasive action through binding with its membrane receptor , promoting pericellular proteolysis and mediating cell signal transduction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , its inhibitors ( PAI 1 and PAI 2 ) , and its receptor ( uPAR ) play a key role in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study we determined the in vitro effects of polysulfated glycosaminoglycan ( PSGAG ) and the glucocorticoid triamcinolone acetonid ( TA ) on the IL 1 altered expression and activity of matrix metalloproteinases ( MMP 1 , MMP 3 ) , tissue inhibitor of metalloproteinases 1 , the plasminogen activators tPA and uPA and plasminogen activator inhibitor 1 by articular chondrocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TCDD induced the early appearance of ovarian plasminogen activator inhibitor type 1 ( PAI 1 ) , plasminogen activator inhibitor type 2 ( PAI 2 ) , urokinase plasminogen activator ( uPA ) , and tissue plasminogen activator ( tPA ) at 24h after dosing when compared with controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Osteoblasts were characterized by measuring osteocalcin , cellular alkaline phosphatase ( ALP ) , and urokinase plasminogen activator ( uPA ) activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease causally involved in invasion and metastasis . ^^^ Paradoxically , high concentrations of plasminogen activator inhibitor ( PAI 1 ) , an endogenous inhibitor of uPA , also correlate with poor prognosis in patients with breast cancer , including the subgroup with node negative disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Quantitative real time reverse transcription PCR ( RT PCR ) assays were developed to quantify urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and tissue metalloproteinase inhibitor type 1 ( TIMP 1 ) mRNA in 54 breast cancer tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
DESIGN AND METHODS : The ability of bone marrow plasma cells to adhere to VN and FN and the consequent formation of focal adhesion plaques on the cell surface , their composition and phosphorylation of several signal transduction proteins , cell proliferation and secretion of matrix metalloproteinase 2 ( MMP 2 ) and 9 ( MMP 9 ) and urokinase type plasminogen activator ( uPA ) were studied . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator system , including urokinase type plasminogen activator ( uPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) , plays an important role in repair of the peritoneum damaged by several types of peritonitis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a multifunctional protein that has been implicated in several physiological and pathological processes involving cell adhesion and migration in addition to fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The circulating urokinase plasminogen activator ( uPA ) and its soluble receptor ( suPAR ) are not up regulated by the circulating P 105 fraction of the HER 2 / neu proto oncogene : in vivo evidence from patients with advanced non small cell lung cancer ( NSCLC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine whether these mediators have a similar effect on fibrinolysis and the remodeling of the fibrin provisional matrix , we examined the role of cytokines on the regulation of urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) in human skin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our laboratory showed that bikunin , a Kunitz type protease inhibitor , suppresses 4beta phorbol 12 myristate 13 acetate ( PMA ) or tumor necrosis factor alpha ( TNFalpha ) induced urokinase type plasminogen activator ( uPA ) expression in different cell types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The amino terminal fragment ( ATF , Ser ( 1 ) Lys ( 135 ) ) of urokinase type plasminogen activator ( uPA ) containing an epidermal growth factor like ( EGF ) and kringle domain is critically involved in some important functions of uPA , such as receptor binding and chemotactic activity . ^^^ In this report , the effect of ATF on single chain uPA ( sc uPA ) induced plasminogen activation was investigated . ^^^ It was shown that sc uPA induced activation of Glu plasminogen or Lys plasminogen was significantly inhibited in the presence of ATF . ^^^ In addition , sc uPA activation to two chain uPA ( tc uPA ) by Lys plasmin and plasminogen activation to plasmin by tc uPA were both found to be inhibited by ATF . ^^^ In contrast to sc uPA induced Glu or Lys plasminogen activation , sc uPA induced mini plasminogen activation , sc uPA activation by mini plasmin and mini plasminogen activation by tc uPA were not affected by ATF . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enhanced benefit from adjuvant chemotherapy in breast cancer patients classified high risk according to urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( n = 3424 ) . ^^^ The strong prognostic impact of the two invasion factors urokinase type plasminogen activator ( uPA ) and its inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) , in breast cancer has recently been validated at level 1 evidence . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One of the key players in this machinery appears to be the urokinase type plasminogen activator ( uPA ) / uPA receptor system . ^^^ The role of uPA and its receptor ( CD 87 ) in plasminogen ( Plg ) activation , cell adhesion , and chemotaxis is well established ; however , less is known of how these activities are regulated . ^^^ Expression of human CD 222 in CD 222 / mouse fibroblasts down regulated Plg activation , cell adhesion , and chemotaxis induced by the uPA / CD87 system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We found that mLRP1B4 and urokinase plasminogen activator receptor ( uPAR ) form immunoprecipitable complexes on the cell surface in the presence of complexes of uPA and its inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytic cleavage of the urokinase plasminogen activator receptor ( uPA ( R ) ) prevents the binding of uPA and vitronectin while generating biologically active uPAR fragments . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Invasion factors urokinase type plasminogen activator ( uPA ) and its plasminogen activator inhibitor ( PAI 1 ) are the only novel tumor biological prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a member of the serine protease family and can break down various components of the extracellular matrix to promote growth , invasion , and metastasis of several malignancies including breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type and tissue type plasminogen activators ( uPA , tPA ) are key enzymes for starting the plasminogen system , which plays important roles in various physiological and pathological conditions . ^^^ In a rabbit model of renal ischemia / reperfusion ( I / R ) , the expression of uPA and tPA mRNAs was down regulated and that of their physiological inhibitor , type 1 plasminogen activator inhibitor , mRNA was up regulated in ischemic kidney compared to non ischemic kidney . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition to aspartyl and cysteineproteinases , serine proteinases including the plasminogen activator system ( uPA , uPAR , tPA , PAI 1 and PAI 2 ) and matrix metalloproteinases ( MMPs ) with their tissue inhibitors ( TIMPs ) play an essential role in these processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HRGbeta 1 was also able to decrease the activity of urokinase type plasminogen activator ( uPA ) and matrix metalloproteinase 9 ( MMP 9 ) , 2 key enzymes in the invasion and metastatic cascade . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Its major components are urokinase ( uPA ) and tissue type plasminogen activator ( tPA ) , plasminogen activation inhibitor type 1 and 2 ( PAI 1 and PAI 2 ) and a receptor for urokinase ( uPAR ) . ^^^ The main findings of this study are that Spitz naevi , firstly , may express plasminogen activator ( tPA ) , inhibitors and the receptor of the PA system , but in a much smaller proportion than cutaneous melanomas ; and secondly , do not express urokinase , whereas some of the melanomas do . uPA positivity may therefore be suggestive of melanoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , we describe an assay for the assessment of components of the plasminogen activation system ( uPA , tPA and PAI 1 , and their complexes ) in blood which is not susceptible to interference by heterophilic antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We previously demonstrated that expression of an albumin urokinase type plasminogen activator ( Alb uPA ) fusion construct in transgenic mice resulted in elevated plasma uPA concentration , hypofibrinogenemia , and neonatal hemorrhaging . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , the antigen levels of urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 , and their immunohistochemical staining were compared in paired colorectal tumor ( n = 64 ) and background colon tissue of the same patients with clinical and pathological staging . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We compared alveolarization in the rat neonate under normal vs . hyperoxic conditions , examining gelatinase , transforming growth factor ( TGF ) beta , and the protease urokinase type plasminogen activator ( uPA ) activities in whole lung and cultured type 2 alveolar epithelial cells ( AEC 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of mRNA for the plasminogen activators ( uPA and tPA ) and their inhibitor ( PAI 1 ) were analyzed by RT PCR ELISA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Treatment of small airway epithelial ( SAEC ) cells or lung epithelial ( Beas2B ) cells with TNF alpha or PMA induces urokinase type plasminogen activator ( uPA ) expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : The fibrinolytic activities of conditioned medium and cell lysates from human glioma cell lines , A 172 , T98G , U 87 and TM 1 were studied by fibrin plate zymography . mRNA expression of tissue plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) was measured by Northern blot analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Extracellular proteolytic enzymes of the urokinase type plasminogen activator ( uPA ) system and the family of metalloproteases play a crucial role in the matrix degradation and tissue remodelling processes characteristic of malignant disorders . ^^^ The receptor for urokinase plasminogen activator ( uPAR ) serves to localise and intensify the action of uPA and is expressed on the surface of malignant as well as tumour stromal cells including fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we note that urokinase type plasminogen activator ( uPA ) is located within this locus . ^^^ Previously , we reported that uPA and its functional homolog , tissue type plasminogen activator , are induced by Abeta treatment of neurons in vitro as well as in a mouse model of Abeta accumulation in vivo . ^^^ Here , we have evaluated the effects of uPA and plasminogen on Abeta fibril formation and neurotoxicity . ^^^ We report that the combination of uPA and plasminogen , but neither alone , inhibits Abeta toxicity , reduces Abeta deposition in vitro , and inhibits Abeta fibrillogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We describe and compare the pH dependencies of the potencies and of the bound structures of two inhibitor isosteres that form multicentered short hydrogen bond arrays at the active sites of trypsin , thrombin , and urokinase type plasminogen activator ( urokinase or uPA ) over certain ranges of pH . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) binds with high affinity to its specific cell surface receptor ( uPAR ) ( CD 87 ) via a well defined sequence within the N terminal region of uPA ( uPA ( 19 31 ) ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) is able to cleave its cell surface receptor ( uPAR ) anchored to the cell membrane through a glycophosphatidylinositol tail . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) are thought to play critical roles in vascular remodeling after injury , with tPA mediating intravascular clot lysis and uPA modulating cell migration within the vessel wall . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human urokinase type plasminogen activator ( uPA ) is poorly secreted by yeast but the mechanisms interfering with its secretion are largely unknown . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human urokinase type plasminogen activator ( uPA or uPA ) has been implicated in the regulation and control of basement membrane and interstitial protein degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
If this is indeed true , then we would expect increased urokinase type plasminogen activator ( uPA ) expression to result in a similar reduction in scarring and improvement in mortality . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We reported previously that down regulating or functionally blocking alphav integrins inhibits endogenous p 38 mitogen activated protein kinase ( MAPK ) activity and urokinase plasminogen activator ( uPA ) expression in invasive MDA MB 231 breast cancer cells whereas engaging alphav integrins with vitronectin activates p 38 MAPK and up regulates uPA expression ( Chen , J . , Baskerville , C . , Han , Q . , Pan , Z . , and Huang , S . ( 2001 ) J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activated transcription of the urokinase type plasminogen activator ( uPA ) gene depends on the enhancer , located approximately 2 kb from the start of transcription . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Invasive squamous cell carcinoma ( SCC ) cells degrade extracellular matrix ( ECM ) via an extracellular protease cascade that includes urokinase type plasminogen activator ( uPA ) , plasmin , and the metalloprotease ( MMP ) family of collagenases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we show that oncostatin M ( OSM ) and interleukin 1 ( IL 1 ) regulate the expression of plasminogen activator inhibitor 1 ( PAI 1 ) and urokinase type plasminogen activator ( uPA ) in human astrocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In our study , we have studied the effects of CD 44 stimulation by ligation with HA upon the expression of matrix metalloproteinases ( MMPs ) 2 and 9 as well as urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) and its inhibitor ( PAI 1 ) and the subsequent induction of invasion of human chondrosarcoma cell line HCS 2 / 8 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor tissues from 71 patients with CRC were assayed to determine the antigen levels of urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , and plasminogen activator inhibitor 1 and 2 ( PAI 1 and PAI 2 ) , as well as immunohistochemical expression of VEGF . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Synthesis , solution structure , and biological evaluation of urokinase type plasminogen activator ( uPA ) derived receptor binding domain mimetics . ^^^ Tumor cell migration and metastasis in cancer are facilitated by interaction of the serine protease urokinase type plasminogen activator ( uPA ) with its receptor uPAR ( CD 87 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have recently demonstrated that phosphatidylinositol 3 kinase ( PI 3 kinase ) and nuclear factor kappaB ( NF kappaB ) regulate motility of highly invasive human breast cancer cells by the secretion of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulated cell movement is modulated by proteases and their associated molecules , including the serine proteases urinary type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) and their inhibitors ( PAIs ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of urokinase type plasminogen activator ( uPA ) to its receptor ( uPAR ) on the surface of tumor cells is involved in the activation of proteolytic cascades responsible for the invasiveness of those cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The hypothesis of this study was that EPC devices increase the level of urokinase plasminogen activator ( uPA ) , a second activator of fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and vitronectin activate cell signaling pathways by binding to the uPA receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : CSF and serum concentrations of urokinase plasminogen activator ( urokinase [ uPA ] ) , uPA receptor ( uPAR ) , and PA inhibitor 1 ( PAI 1 ) were quantified by ELISA in 12 patients with bacterial meningitis , control patients ( n = 10 ) with noninflammatory neurologic diseases , and 10 patients with Guillain Barr syndrome ( GBS ) , a disease in which blood CSF barrier disruption occurs without CSF pleocytosis . ^^^ RESULTS : A marked increase in uPA dependent plasminogen activation was detected in the CSF of patients with bacterial meningitis vs CSF of patients with GBS and controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We analysed the distribution of genotypes of two polymorphisms in the urokinase type plasminogen activator ( uPA ) gene : C > T substitution in exon 6 and T > C substitution in intron 7 in 52 subjects with colorectal cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have examined the role of fibrinolytic factors , i . e . , tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , and their substrate , plasminogen , in the proliferation of hepatocytes in primary culture . ^^^ DNA synthesis was assessed by measuring the incorporation of [ 3H ] thymidine into cellular DNA fraction . tPA , uPA and type 1 plasminogen activator inhibitor ( PAI 1 ) gene expressions were measured by Northern blotting . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen directs the pleiotropic effects of uPA in liver injury and repair . ^^^ The urokinase type plasminogen activator ( uPA ) plays a central role in liver repair . ^^^ Here , we define the molecular mechanisms of liver injury and explore whether uPA can regulate liver repair independently of plasminogen . ^^^ To address the hypothesis that the liver injury in transgenic mice results from the intracellular activation of plasminogen by transgene derived uPA ( uPAT ) , we generated mice that overexpress uPAT and lack functional plasminogen ( uPAT Plg ( ) ) . ^^^ Notably , high levels of circulating uPA in uPAT Plg ( ) mice did not prevent the long term extrahepatic abnormalities previously associated with plasminogen deficiency . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Finally , we demonstrate that urokinase plasminogen activator ( uPA ) , an NF kappa B target gene , is induced by CD 40 ; and conversely , that uPA induction via CD 40 is blocked by PI3K and NF kappa B inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serpin plasminogen activator inhibitor type 1 ( PAI 1 ) , as the primary physiological inhibitor of both urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activator , plays an important role in the regulation of the fibrinolytic system as well as in extracellular remodeling in both physiological and pathophysiological processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We hypothesized that the relative balance between urokinase type plasminogen activator ( uPA ) and PAI 1 expression in favor of uPA prevents scarring in early fetal skin wounds , whereas a change in favor of PAI 1 in late gestation results in wound scarring . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Matrix metalloproteases ( MMPs ) and urokinase type plasminogen activator ( uPA ) were quantified with zymography and Matrigel coated Transwell was employed to estimate the invasiveness of SNU 16 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The regulated expression of the urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor ( PAI 1 ) is believed to modulate the invasive capacity of human trophoblastic cells in vitro and in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression levels of the alpha3beta1 integrin , utilized for attachment to laminin 5 and other matrix proteins , was high in SCC 68 cells , moderate in SCC 15 cells , and low in ppl 26 cells . alpha3beta1 integrin clustering up regulates expression of urinary type plasminogen activator ( uPA ) in ppl 26 cells via a mechanism involving ERK activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Syk , PI 3 ' kinase inhibitors , and NF kappa B inhibitory peptide inhibited urokinase type plasminogen activator ( uPA ) secretion and cell motility in these cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We prepared diphtheria toxin / urokinase fusion protein ( DTAT ) composed of the amino terminal fragment of the urokinase type plasminogen activator ( uPA ) fused to the catalytic and translocation domains of diphtheria toxin ( DT ) and assessed its activity on leukemic cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) regulates the remodeling of extracellular matrix and controls reparative processes such as wound healing and liver regeneration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A 6 is an eight amino acid peptide derived from the non receptor binding region of urokinase plasminogen activator ( uPA ) , which interferes with the uPA / uPA receptor system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although we could confirm the presence of urokinase plasminogen activator ( uPA ) in STBM we could demonstrate no intrinsic activity presumably because of its association with the plasminogen activator inhibitor 2 ( PAI 2 ) which is also a component of STBM . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Matrix metalloproteinase 9 ( MMP 9 ) and urokinase type plasminogen activator ( uPA ) are key extracellular matrix proteases involved in the invasion of cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This protease activates hepatocyte growth factor ( HGF ) and urokinase plasminogen activator ( uPA ) , two proteins thought to be involved in the growth and motility of cancer cells , particularly carcinomas , and in the vascularization of tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mice carrying an albumin urokinase type plasminogen activator transgene ( AL uPA ) develop liver disease secondary to uPA expression in hepatocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : Trophoblast proteases , matrix metalloproteases ( MMP 2 , MMP 9 ) , membrane type matrix metalloproteases ( MT MMP 1 & 2 ) and urokinase type plasminogen activator ( uPA ) were found to be up regulated by IL 1beta while the protease inhibitors , tissue inhibitor of metalloproteases ( TIMP 1 & 2 ) and plasminogen activator inhibitors ( PAI 1 & 2 ) to be up regulated by TGF beta 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) belongs to a family of proteins that contains kringle domain and plays an important role in inflammation , tissue remodeling , angiogenesis , and tumor metastasis by pericellular plasminogen activation . ^^^ It did not block plasminogen activation by activated uPA in clot lysis and chromogenic substrate assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The roles of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in the formation of macroscopic types and invasion were investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We previously reported that anthracyclines , which could generate reactive oxygen species ( ROS ) , could induce the urokinase type plasminogen activator ( uPA ) gene expression in human RC K 8 malignant lymphoma cells and in H 69 small cell lung cancer ( SCLC ) cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have chosen plasminogen activator inhibitor ( s ) type 1 ( PAI 1 ) , which selectively inhibits the urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The reciprocal pro enzyme activation system of plasmin , urokinase type plasminogen activator ( uPA ) and their respective zymogens is a potent mechanism in the generation of extracellular proteolytic activity . ^^^ This system is complicated by a poorly understood intrinsic reactivity of the uPA pro enzyme ( pro uPA ) before proteolytic activation , directed against both plasminogen and PAI 1 . ^^^ However , in spite of this very fast inhibition , two chain uPA still became the dominant plasminogen activator when plasminogen was incubated with pro uPA and PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To detect the expression and evaluate the clinical significance of urokinase type plasminogen activator ( uPA ) in medulloblastoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expression of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) in the synovial tissures , and the significance of uPA and uPAR in degradation of extracellular matrices in osteoarthritis ( OA ) , then analyse the possible relationship between uPA and uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A variety of serine proteases , including urokinase type plasminogen activator ( uPA ) , plasmin , and polymorphonuclear leukocyte elastase ( PMN E ) , have been implicated in the processes of tumor cell invasion and metastasis . ^^^ Besides degrading of matrix proteins , PMN E has been shown to be able to cleave and inactivate plasminogen activator inhibitor 1 ( PAI 1 ) , the main inhibitor of uPA , and alpha 2 antiplasmin , the natural inhibitor of plasmin , thus enabling an uncontrolled matrix degradation by the fibrinolytic enzymes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) is a serine protease that can initiate proteolytic cascades , which result in remodeling of extracellular matrix and basement membrane , allowing cells to move across and through these barriers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Invasion of inflammatory cells and hitherto the rest of the wound healing cascade is inhibited in plasminogen or uPA deficient mice , most likely by the inhibition of MMP activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both urokinase like plasminogen activator ( uPA ) and its inhibitor plasminogen activator inhibitor ( PAI 1 ) , as well as uPA PAI 1 complexes , have been identified as important prognostic factors in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Bikunin ( bik ) , a Kunitz type protease inhibitor , also known as urinary trypsin inhibitor , is proposed as a main participant in the inhibition of tumor cell invasion and metastasis , possibly through the direct inhibition of cell associated plasmin activity and suppression of urokinase type plasminogen activator ( uPA ) mRNA expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mammalian urokinase type plasminogen activator ( uPA ) is produced as a stable single polypeptide chain zymogen and requires a distinct proteolytic cleavage to become an active , two chain enzyme . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stromal cells also contribute to breast cancer growth and metastasis through the production of extracellular matrix ( ECM ) modifiers such as urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) , its inhibitors ( PAI 1 and PAI 2 ) , matrix metalloproteinases ( MMPs ) , and growth factors , including the fibroblast and insulin like growth factors ( FGF ' s and IGF ' s ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Besides routine pathology for tissue and BM , CK+ BM cells were detected by immunocytochemistry ( IC ) and 4 different tumour markers as well as the shedded domain of the oncoprotein Her 2 / neu and urokinase plasminogen activator uPA were determined by radio or enzyme immunoassay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased expression of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) is associated with an ingress of monocytes into the thrombus . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Confluent monolayers of bovine retinal microvascular endothelial ( BRE ) cells grown on porous membrane were treated with VEGF or urokinase plasminogen activator ( uPA ) , and permeability changes were analyzed . uPA induced permeability was rapid and sustained , but VEGF induced permeability showed a biphasic pattern : a rapid and transient phase ( 1 2 h ) followed by delayed and sustained phase ( 6 24 h ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously demonstrated that urokinase type plasminogen activator ( uPA ) is highly expressed in the aneurysmal segment of the abdominal aorta ( AAA ) in apolipoprotein E deficient ( apoE / ) mice treated with angiotensin 2 ( Ang 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These findings indicated that the EA fraction suppressed tumour cell invasion by downregulation of uPA ( urokinase type plasminogen activator ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We provide evidence that plasminogen activator inhibitor ( PAI ) 1 can detach cells by disrupting uPAR VN and integrin VN interactions and that it does so by binding to the uPA present in uPA uPAR integrin complexes on the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Protein expression of the PAS , including urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) , plasminogen ( Plg ) , and plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) , was determined in the colonic tissue samples of 56 patients with resected primary CRC by quantitative immunohistochemistry and correlated with clinicopathological parameters and patient outcome . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have investigated whether there are any differences in the release of urokinase plasminogen activator ( uPA ) and its inhibitor ( PAI 1 ) from cultured endometrial and endometriotic stromal cells , and whether the release is regulated by epidermal growth factor ( EGF ) or transforming growth factor beta 1 ( TGFbeta 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) strongly correlates with a malignant tumour cell phenotype . ^^^ Antisense transfection of HCT 116 cells had no effect on proliferation but the following effects were observed : ( 1 ) a 1 . 3 fold decreased adhesion ; ( 2 ) a two fold decreased Erk MAP kinase activity ; ( 3 ) a 2 . 7 fold decrease in Src kinase activity ; ( 4 ) a 1 . 5 and two fold decrease in uPA cell surface expression and secretion ; ( 5 ) abrogation of promatrix metalloproteinase 9 secretion ; and ( 6 ) a complete suppression of plasminogen dependent matrix degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : The independent clinical relevance of invasion factors urokinase type plasminogen activator ( uPA ) / PAI 1 and HER 2 status was evaluated in lymph node negative breast cancer patients ( N = 118 ) without adjuvant systemic therapy after long term follow up of more than 10 years ( median , 126 months ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen / plasmin system , urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) , and its inhibitor ( PAI 1 ) , influence extracellular proteolysis and cell migration in lung injury or neoplasia . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PAI 2 targets the cell surface receptor bound urokinase plasminogen activator ( uPA ) , which is involved with the metastatic spread of cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) system , which consists of a proteinase ( uPA ) , a receptor ( uPAR or CD 87 ) and inhibitors , is involved in proteolysis , cell migration , tissue remodelling , angiogenesis and cell adhesion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mice without uPA , tPA , or plasminogen genes are resistant to experimental choroidal neovascularization . ^^^ The influence of endogenous plasminogen ( Plg ) , urokinase ( uPA ) , tissue type plasminogen activator ( tPA ) , and uPA receptor ( uPAR ) was explored in single gene deficient mice in a model of laser induced CNV . ^^^ The absence of Plg , uPA , or tPA significantly decreased the development of experimental CNV compared with wild type or uPAR deficient mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of plasminogen to leukocytic cell lines induces a 30 to 80 fold increase in the rate of plasminogen activation by tissue type ( tPA ) and urokinase type ( uPA ) plasminogen activators . ^^^ We produced and characterized a monoclonal antibody ( MAb ) 11G1 against purified alpha enolase , which abrogated about 90 % of cell dependent plasminogen activation by either uPA or tPA on leukocytoid cell lines of different lineages : B lymphocytic , T lymphocytic , granulocytic , and monocytic cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) are instrumental in cellular activities during inflammation , angiogenesis and tumor metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Depression of fibrinolytic activity occurs as a result of inhibition of urokinase plasminogen activator ( uPA ) by plasminogen activators , or series inhibition of plasmin by antiplasmins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , a trypsin like serine proteinase , plays an important role in normal tissue re modelling , cell adhesion , and cell motility . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) and its inhibitors type 1 ( PAI 1 ) and type 2 ( PAI 2 ) are considered to have a key role in the process of invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and metalloproteinases ( MMP ) play key roles in invasion and metastasis , degrading extracellular matrix compounds and modulating tumor cell motility . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To define the role of the plasminogen activators ( PAs ) urokinase PA ( uPA ) and tissue PA ( tPA ) as well as the uPA receptor ( uPAR ) in arteriogenesis , we investigated their impact in a rabbit and mouse model of adaptive collateral artery growth . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This allows plasminogen to be co localised in an activation susceptible form with the enhanced uPA levels seen in malignancy and together furnishes tumor cells with elevated tissue remodeling capacity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor uPAR are components of the fibrinolytic system and are important for an adequate immune response to respiratory tract infection , in part through their role in the migration of inflammatory cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Therefore , the purpose of this investigation was to determine if differences in the matrix metalloproteinase ( MMP ) 2 and 9 , tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) protein and activity levels existed between infrarenal AAA and normal abdominal aortic tissue specimens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purpose of this study was to evaluate the roles of matrix metalloproteinases ( MMPs ) and urokinase type plasminogen activator ( uPA ) with regard to varicose veins ( VVs ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) and its cellular receptor ( uPAR ) are involved in the proteolytic cascade required for tumor cell dissemination and metastasis , and are highly expressed in many human tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this study was to determine the expression of proteinases and inhibitors from the matrix metalloproteinase ( MMP ) ( MMPs 1 , 2 , 3 , 9 , tissue inhibitors of metalloproteinases ( TIMPs ) 1 , 2 ) and plasminogen activator ( ( PA ) urokinase ( uPA ) , tissue type ( tPA ) , uPAR , plasminogen activator inhibitors ( PAIs ) 1 , 2 ) systems in colorectal cancer pathology by gelatin zymography , enzyme linked immunosorbent assays ( ELISAs ) and quenched fluorescent substrate hydrolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical studies revealed that although dermal fibroblasts of normal scars and keloids expressed both urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , keloid fibroblasts had a much higher PAI 1 expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase cellular receptor ( uPAR ) recognizes the N terminal growth factor domain of urokinase type plasminogen activator ( uPA ) and is expressed by several cell types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cancer tissues from 101 gastric cancer patients were assayed immunohistochemically for expression of urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , PA inhibitor 1 ( PAI 1 ) and VEGF protein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It has been shown in an in vitro system that plasma kallikrein activates pro urokinase ( pro uPA ) and / or plasminogen , resulting in plasmin generation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator ( uPA ) promotes matrix degradation by many cell types , including the invasive extravillous trophoblast ( EVT ) of the human placenta . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVES : The urokinase plasminogen activation ( uPA ) system plays an important role in tumor invasion and metastasis by mediating proteolysis , adhesion , and migration of tumor cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The active site hydrogen bonding mode was found to vary markedly with pH , with the steric and electronic properties of the inhibitor , and with the type of protease ( trypsin , thrombin or urokinase type plasminogen activator ( uPA ) ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The processes of ovarian cancer dissemination are characterized by altered local proteolysis , cellular proliferation , cell attachment , and invasion , suggesting that the urokinase type plasminogen activator ( uPA ) and its specific inhibitor ( plasminogen activator inhibitor type 1 ( PAI 1 ) ) could be involved in the pathogenesis of peritoneal dissemination . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A high cytosol value of urokinase type plasminogen activator ( uPA ) may be predictive of early relapse in primary breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Concentrations of total and active transforming growth factor beta 1 ( TGF beta 1 ) , tumor necrosis factor alpha ( TNF alpha ) , tissue type plasminogen activator ( t PA ) , urokinase plasminogen activator ( uPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) were obtained . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We now report new interactions between p 97 and another LRP ligand , the urokinase plasminogen activator ( uPA ) complex . ^^^ By using biospecific interaction analysis , both pro uPA and plasminogen are shown to interact with immobilized p 97 . ^^^ Moreover , the activation of plasminogen by pro uPA is increased by soluble p 97 . ^^^ These results indicate that membrane bound and soluble p 97 affect the migration capacity of endothelial and melanoma cells and suggest that p 97 could be involved in the regulation of plasminogen activation by interacting with pro uPA and plasminogen . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of urokinase plasminogen activator ( uPA ) to its receptor ( uPAR ) initiates a proteolytic cascade facilitating the activation of matrix metalloproteinase 9 ( MMP 9 ) , which in turn degrades the extracellular matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) induces cell adhesion and chemotactic movement . uPA signaling requires its binding to uPA receptor ( uPAR / CD87 ) , but how glycosylphosphatidylinositol anchored uPAR mediates signaling is unclear . uPAR is a ligand for several integrins ( e . g . alpha 5 beta 1 ) and supports cell cell interaction by binding to integrins on apposing cells ( in trans ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Amyloid beta protein stimulates the expression of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) in human cerebrovascular smooth muscle cells . ^^^ RNase protection assays and plasminogen zymography showed that urokinase type plasminogen activator ( uPA ) was responsible for this activity . ^^^ On the other hand , chronic expression of uPA and plasminogen activation led to a profound loss of HCSM cell attachment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effects of hepatocyte growth factor on urokinase type plasminogen activator ( uPA ) and uPA receptor in DU 145 prostate cancer cells . ^^^ Urokinase type plasminogen activator ( uPA ) and the uPA receptor ( uPAR ) are involved in a proteolytic cascade resulting of extracellular matrix degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease that catalyzes the conversion of plasminogen to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase type plasminogen activator ( uPAR ) plays important roles in a number of physiological and pathological processes by virtue of its interactions with urokinase type plasminogen activator ( uPA ) , vitronectin ( Vn ) , and several other proteins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Angiogenesis requires tightly controlled extracellular matrix degradation mediated by extracellular proteolytic enzymes including matrix metalloproteinases ( MMPs ) and serine proteases , in particular , the urokinase type plasminogen activator ( uPA ) plasmin system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mechanism of pro MMP 2 activation in glioma astrocyte coculture was investigated and was found to involve the urokinase type plasminogen activator ( uPA ) plasmin cascade whereby uPA bound to uPA receptor ( uPAR ) , leading to the conversion of plasminogen to plasmin . ^^^ Furthermore , key components ( i . e . , uPAR , uPA , and pro MMP 2 ) were contributed principally by astrocytes , whereas the U251N glioma cells provided plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We show that the reduction of AR expression allowed to an important decrease of the levels of urokinase type plasminogen activator ( uPA ) and transforming growth factor beta 1 ( TGFbeta 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytic degradation of fibrin ( fibrinolysis ) is mediated by plasminogen and its activators , tissue type plasminogen activator ( tPA ( 1 ) ) and urokinase ( uPA ) . ^^^ Plasminogen activator inhibitor 1 ( PAI 1 ) , a member of the serine protease inhibitor ( serpin ) superfamily , is the principal inhibitor of tPA and uPA in the fibrinolytic system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , the molecular mechanism by which upstream kinases regulate the OPN induced NFkappaB activation and urokinase type plasminogen activator ( uPA ) secretion in human breast cancer cells is not well defined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) promotes tumor invasion and metastasis in several malignancies including prostate cancer , one of the most commonly detected male cancers that result in a high incidence of mortality . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To detect the expression of urokinase type plasminogen activator ( uPA ) and its inhibitors ( plasminogen activator inhibitors , PAI ) type 1 and type 2 in squamous cell carcinoma of human larynx and reveal the correlation of the major clinicopathologicl parameters and prognosis . ^^^ PAI 1 might act as an essential modulator of the plasminogen activation system or a protector of carcinoma tissue against self degradation rather than as a simple inhibitor of system . uPA and PAI 2 could be new independent and strong biologically prognostic factors . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The subject of this review , the urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) , plays an impressive range of distinct , but overlapping functions in the process of cancer invasion and metastasis : Firstly , uPA / uPAR promotes extracellular proteolysis by regulating plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The levels of urokinase ( uPA ) and tissue type ( tPA ) plasminogen activators and their type 1 inhibitor ( PAI 1 ) were determined by immunoassay in tumor cytosols and samples of histologically unaltered adjacent mucosa in gastric cancer patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A random sample of 70 of the 112 cases had plasma levels of urokinase like plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , macrophage inhibiting factor ( MIF ) , tumour growth factor beta 1 ( TGF beta 1 ) , homocysteine , and serum levels of IgA antibodies against Chlamydia pneumoniae ( IgA CP ) and Cotinine ( a nicotine metabolite ) measured . ^^^ CONCLUSION : the aortic matrix degradation in AAA may be partly caused by an activation of plasminogen by tPA , but apparently not by uPA , which usually dominates matrix degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Of particular importance , urokinase type plasminogen activator ( uPA ) has been shown to correlate with human prostate cancer ( PC ) metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
For instance , the cell surface receptor uPAR ( CD 87 ) and its ligand , the serine protease urokinase type plasminogen activator ( uPA ) , facilitate tumor invasion and metastasis in solid malignant tumors . ^^^ Besides its proteolytic function in activating the zymogen plasminogen into the serine protease plasmin , binding of uPA to tumor cell associated uPAR initiates various cell responses such as tumor cell migration , adhesion , proliferation , and differentiation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The tumor cell associated urokinase type plasminogen activator system , consisting of the serine protease uPA , its substrate plasminogen , its membrane bound receptor uPAR , as well as its inhibitors PAI 1 and PAI 2 , plays an important role in these pericellular processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Clinically relevant molecules whose expression or structure is altered include the plasminogen activator ( uPA ) and its inhibitor PAI 1 ( plasminogen activator inhibitor type 1 ) , the cell cycle regulator cyclin E , epidermal growth factor ( EGF ) , the apoptosis inhibitor bcl 2 , the cell adhesion molecule E cadherin , and the multifunctional protein beta catenin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) activity in retinal extracts was determined by plasminogen / fibrinogen zymography . ^^^ We found a correlation between the increased uPA activity , and conversion of zymogen plasminogen to active plasmin in retinal extracts with laminin degradation in the retina and apoptosis of ganglion cells . ^^^ In addition , uPA or tPA failed to degrade laminin in control retinal extracts unless plasminogen was added , indicating that plasminogen activation is necessary for laminin degradation , in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The tumor cell surface associated urokinase type plasminogen activator ( uPA ) system plays an especially important role in tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interaction between urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) localizes cellular proteolysis and promotes cellular proliferation and migration , effects that may contribute to the pathogenesis of lung inflammation and neoplasia . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In cancers , administration of bikunin may block tumor cell invasion by a direct inhibition of tumor cell associated plasmin activity as well as by inhibiting urokinase type plasminogen activator ( uPA ) expression at the gene and protein levels , possibly through suppression of CD 44 dimerization and / or the MAP kinase signaling cascade . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
CD 87 is a widely expressed receptor for urokinase plasminogen activator ( uPA ) and plays a critical role in regulation of cell surface plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen binds to surface displayed pneumococcal alpha enolase ( Eno ) and is subsequently activated to the serine protease plasmin by host derived tissue plasminogen activator ( tPA ) or urokinase ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Results from two groups reveal a role for PAI 1 in promoting cycles of attachment and detachment of the cell from the extracellular matrix that is independent of its role as an enzymatic inhibitor of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : The aim of this study was to reveal the relations between expression and tumor behavior such as invasion , metastasis and prognosis in oral squamous cell carcinoma ( SCC ) through analyzing the expression of urokinase type plasminogen activator ( UPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : The aim of this study was to observe the expression of urokinase type plasminogen activator ( UPA ) in human tongue squamous cell carcinoma cell line ( Tca 8113 ) after heat shock with different dosage . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Vascular endothelial cells ( EC ) synthesize activators and inhibitors for fibrinolysis , tissue and urokinase plasminogen activators ( tPA and uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prognostic and predictive value of the urokinase type plasminogen activator ( uPA ) and its inhibitors PAI 1 and PAI 2 in operable breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased urokinase plasminogen activator ( uPA ) levels and epidermal growth factor receptor ( EGFR ) related tyrosine kinase activity are associated with poor prognosis in several cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PA , particularly urokinase plasminogen activator ( uPA ) , has been implicated in extracellular proteolysis , local invasiveness , metastatic spread and angiogenesis . ^^^ High levels of uPA and plasminogen activator inhibitor 1 ( PAI 1 ) correlate with poor prognosis in several cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single site mutants within the Asp 355 Arg356 Pro 357 segment of PAI 1 yield guanidine activatable inhibitors ( a ) that can still form SDS stable complexes with tPA and urokinase plasminogen activator ( uPA ) , and ( b ) that have inhibition rate constants towards plasminogen activators which resemble those of the fibrosarcoma inhibitor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To evaluate the role of plasminogen activator inhibitor 1 ( PAI 1 ) , urokinase plasminogen activator ( uPA ) , and tissue type plasminogen activator ( tPA ) in adhesion formation after laparoscopic surgery . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PAI 1 is like the corresponding plasminogen activator uPA ( urokinase type plasminogen activator ) consistently expressed in human breast cancer . ^^^ In these tumors , the expression pattern of uPA and PAI 1 resembles that of human ductal breast cancer and plasminogen is required for efficient metastasis . ^^^ These results agree with the virtual lack of spontaneous phenotype in PAI 1 deficient mice and humans and may reflect that the plasminogen activation reaction is not rate limiting for tumor vascularization and metastasis , or that there is a functional redundancy between PAI 1 and other inhibitors of the uPA / plasmin system , masking the effect of PAI 1 deficiency . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators tPA and uPA are involved in tissue remodeling , but their role in bone growth is undefined . ^^^ To elucidate the involvement of the plasminogen activators tPA and uPA in this process , we characterized the long bone phenotype in mice deficient in both tPA and uPA ( tPA / : uPA / ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator receptor antisense treatment of HCT 116 cells was associated with a 1 . 3 fold inhibition of adhesion , approximately four fold suppression of HMW uPA secretion and inhibition of pro MMP 9 secretion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Of the `` new ' ' tumor biologic parameters , only the measurement of the urokinase type plasminogen activator ( uPA ) and its inhibitor ( PAI 1 ) in the primary tumor of node negative patients has been adequately validated and can therefore be recommended for clinical application . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) activates plasminogen to plasmin and is often associated with diseases where tissue remodeling is essential ( e . g . cancer , macular degeneration , atherosclerosis ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of a high affinity , glycolipid anchored receptor for the urokinase type plasminogen activator ( uPAR ) is often up regulated during such tissue remodeling events . uPAR may , therefore , in cooperation with various matrix metalloproteases , serve to facilitate the proteolytic breakdown of the extracellular matrix via uPA catalyzed plasminogen activation at the foci where cellular invasion occurs . ^^^ Consistent with such a role for uPAR in pericellular proteolysis is the observation that the membrane assembly of both plasminogen , via its lysine binding sites , and of pro uPA , via its tight binding to uPAR , is required to favor and confine plasminogen activation potential in proximity of the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) : development of antagonists of uPA / uPAR interaction and their effects in vitro and in vivo . ^^^ In cancer , increased levels of the tumor associated serine protease uPA ( urokinase type plasminogen activator ) and its receptor uPAR ( CD 87 ) are linked to tumor progression , metastasis , and shortened survival in patients afflicted with this disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the principal inhibitor of urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) , and as such is thought to play an important role in the regulation of extracellular matrix remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The levels of plasminogen activator inhibitor ( PAI ) 1 activity , uPA antigen and factor ( F ) XIIa did not significantly differ between the patient groups and healthy controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To investigate the possible role of urokinase type plasminogen activator ( uPA ) in corneal epithelial wound healing by examining its expression both in the rabbit corneal epithelium in situ and in rabbit corneal epithelial ( RCE ) cells in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purified fusion protein inhibited urokinase type plasminogen activator as measured by milk agarose plate assay , and bound to human lung cancer cells via uPA receptor ( uPAR ) , which was confirmed by radio competition experiments . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Overexpression of urokinase plasminogen activator ( uPA ) is known to correlate closely with tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Type 1 plasminogen activator inhibitor ( PAI 1 ) is the primary inhibitor of tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purpose of this study was to examine the mechanisms involved in amiloride induced attenuation of SMC proliferation and migration , looking specifically at the potential role of apoptosis and urokinase plasminogen activator ( uPA ) activity in these processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , paraffin embedded material from 84 human pituitary adenomas ( acromegaly n=18 , Cushing ' s disease n=21 , prolactinoma n=18 , thyroid stimulating hormone secreting adenoma n=1 , nonsecreting adenoma n=26 ) and 9 nontumourous anterior pituitary lobes ( obtained from patients with prostate cancer ) was immunohistochemically analysed for expression of MMP 2 , MMP 9 , tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) , urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The overexpression of MIC 1 into SNU 216 cells significantly increased the activity of urokinase type plasminogen activator ( uPA ) , and the expressions of uPA and urokinase type plasminogen activator receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Angiogenesis was assessed in gene targeted mice with deficiencies of plasminogen , urokinase plasminogen activator ( uPA ) , and urokinase receptor ( uPAR ) in a mouse corneal model . ^^^ CONCLUSIONS : These results demonstrate the importance of plasminogen , as well as of uPA , in angiogenesis in vivo . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To date , the factors capable of regulating the coordinate expression of the urokinase type plasminogen activator ( uPA ) and its endogenous inhibitor , plasminogen activator inhibitor ( PAI 1 ) , at the maternal fetal interface remain poorly characterized . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) system has been implicated in cell migration , angiogenesis , extracellular matrix synthesis and tissue remodelling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Serp 1 potently inhibits human serum proteases including plasmin , urokinase type plasminogen activator ( uPA ) , and tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The fibrinolytic factors , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) , were increased in the course of spheroid formation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of urokinase type plasminogen activator ( uPA ) has been well documented in the pathogenesis of pemphigus vulgaris ( PV ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The latter effect was not due to a decrease of urokinase type plasminogen activator ( uPA ) , nor of metalloproteinase 2 ( MMP 2 ) activities . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) receptors ( uPAR ) can be engaged for activation signaling either by aggregation or by binding exogenous uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) binds to its receptor , uPAR , on the surface of cancer cells , leading to the formation of plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 2 ( PAI 2 ) is well documented as an inhibitor of the extracellular serine proteinase urokinase type plasminogen activator ( uPA ) and is expressed in activated monocytes and macrophages , differentiating keratinocytes , and many tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Competitive phage display selection yielded a Pkal inhibitor with an apparent dissociation equilibrium constant ( K ( 1 ) * ) of 11 pM , whereas K ( 1 ) * values for related proteases ( such as Factor Xa ( FXa ) , Factor XIa ( FXIa ) , urokinase type plasminogen activator ( uPA ) , thrombin , and membrane type serine protease 1 ( MT SP 1 ) ) were four to seven orders of magnitude higher . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Extracellular proteolytic enzymes of the urokinase type plasminogen activator ( uPA ) system and the family of metalloproteinases ( MMPs ) catalyse the matrix degradation and remodelling processes characteristic of invasive malignant disorders . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) play an important role in cell guidance and chemotaxis during normal and pathological events . uPAR is GPI anchored and the mechanism by which it transmits intracellular polarity cues across the plasma membrane during directional sensing has not been elucidated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Microarray analysis indicated significantly reduced expression of TNF alpha , LPS binding protein , urokinase type plasminogen activator ( uPA ) , IGF , IGF binding protein ( IGFBP ) 3 , 5 , and increased expression of apolipoprotein 4 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) play an important role in the invasiveness of gliomas and other infiltrative tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recently , a recombinant bifunctional inhibitor ( chCys uPA 19 31 ) directed against cysteine proteases and the urokinase type plasminogen activator ( uPA ) / plasmin serine protease system was generated by introducing the uPA receptor ( uPAR ) binding site of uPA into chicken cystatin ( chCysWT ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this report we present an extension of the pooled analysis of the prognostic impact of urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 in breast cancer patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of urokinase type plasminogen activator ( uPA ) / uPA receptor in HIV 1 infection . ^^^ The binding of urokinase type plasminogen activator ( uPA ) to its glycosyl phosphatidyl inositol ( GPI ) anchored receptor ( uPAR ) mediates a variety of functions in terms of vascular homeostasis , inflammation and tissue repair . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : Urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor ( PAI 1 ) , and uPA receptor ( uPAR ) are prognostic factors in various cancer types , especially breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Primary mouse osteoblasts expressed mRNA for urokinase type plasminogen activator ( uPA ) , tIssue type plasminogen activator ( tPA ) , the type 1 receptor for uPA , plasminogen activator inhibitor types 1 and 2 and the broad spectrum serine proteinase inhibitor , protease nexin 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator ( uPA ) , its inhibitor PAI 1 , and its cellular receptor uPA R ( CD 87 ) are of crucial importance during cellular invasion and migration , required for a variety of physio and pathophysiological processes . ^^^ It has become increasingly evident in recent years that the uPA / uPA R system has far more functional properties than plasminogen activation alone . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study was undertaken to evaluate the circulating urokinase plasminogen activator ( uPA ) , and its soluble receptor ( suPAR ) in patients with either small cell lung cancer ( SCLC ) or non small cell lung cancer ( NSCLC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This activity was completely inhibited by plasminogen activator inhibitor 1 and by amiloride , identifying the activity as urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We aimed to investigate the effect of PPARgamma ligands on expression of MMP 1 and urokinase plasminogen activator ( uPA ) in human microvascular endothelial cells ( HMEC 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Active plasmin is generated by proteolytic activation of the zymogen plasminogen ( Plg ) by urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mRNAs of urokinase plasminogen activator ( uPA ) and its receptor , uPAR , contain instability determining AU rich elements ( AREs ) in their 3 ' untranslated regions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Substantial evidence exists which implicates the urokinase plasminogen activator system [ urokinase plasminogen activator ( uPA ) , urokinase plasminogen activator receptor ( uPAR ) and plasminogen activator inhibitor 1 ( PAI 1 ) ] in the neo vascularization , invasion and metastasis of many solid tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PLH had no effect on plasminogen activation , as evidenced by no inhibitions on either uPA amidolytic activity or plasmin formation derived from its zymogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Functional activity of receptor ligand interaction was measured using c Met phosphorylation status , proliferation assays ( anchorage dependent and independent ) , Matrigel invasion , modulation of urokinase plasminogen activator ( uPA ) expression , and cell dispersion ( scattering ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Tumor expression of urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and uPA receptor ( uPAR ) are breast cancer prognostic factors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , which of the two plasminogen activators ( PAs ) present in renal tissue , tissue plasminogen activator ( tPA ) or urokinase type plasminogen activator ( uPA ) , is responsible for plasmin generation and those factors that modulate the activity of this system remain unclear . ^^^ This study utilized mesangial cells isolated from mice with gene deletions for tPA , uPA , and plasminogen activator inhibitor 1 ( PAI 1 ) to further delineate the role of the PA / plasminogen / plasmin system in ECM accumulation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : In inflammation , urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) play an important role in fibrinolysis and in activation and chemotaxis of neutrophils and lymphocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Luciferase reporter gene assay indicated that Lck induces NFkappaB dependent urokinase type plasminogen activator ( uPA ) promoter activity in presence of H / R . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In a retrospective study of 488 women with primary breast cancer , after a median follow up of 10 years , we sought interactions between disease free survival ( DFS ) and overall survival ( OS ) and tumor antigen levels of two components of the plasminogen system , urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 , and the transmembrane growth factor receptor c erbB 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interestingly , urokinase plasminogen activator ( uPA ) and and PA inhibitor 1 ( PAI 1 ) levels were not significantly affected by HA of increasing MW ; however , the PAI 1 to uPA ratio showed a slight , yet nonsignificant increase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Introduction into each of these loops of sequences derived from the amino terminal fragment of urokinase plasminogen activator ( uPA ) or a related phage display peptide reduced the solubility of VP 1 molecules when expressed in insect cells , and insertions into the EF loop reduced VP 1 solubility least . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Twenty PVR , PDR or pucker membranes were examined to identify the cells with cell specific markers and to detect the expression of urokinase ( uPA ) , tissue type plasminogen activator ( tPA ) or plasminogen activator inhibitor 1 ( PAI 1 ) by in situ hybridization and by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator ( urinary plasminogen activator , urokinase ) ( uPA ) and its PA 1 type 1 inhibitor are not only prognostically but also predictively significant and support clinical decisions on therapy in primary carcinoma of the breast ] . uPA and PAI 1 are the first novel tumor biological prognostic factors in breast cancer for which the prognostic impact has been validated at the highest level of evidence and hence all evaluation criteria for transfer into clinical practice have been fullfilled . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have shown previously that cytoskeletal reorganization ( CSR ) induced by pharmacological reagents such as colchicine or cytochalasins can up regulate the urokinase type plasminogen activator ( uPA ) gene via the Ras / Erk signaling pathway . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A hallmark of pancreatic cancer is the loss of basement membrane that may be attributed to the action of urinary plasminogen activator ( uPA ) and matrix metalloproteinase 9 ( MMP 9 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The authors found previously that plasma levels of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) were elevated in patients with bladder carcinoma and were associated with features of biologically aggressive disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TGF beta induced the enhanced expression of matrix metalloproteinase 9 ( MMP 9 ) and urokinase type plasminogen activator ( uPA ) in GCTM 1 at both the protein and enzymatic activity levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of plasminogen activator inhibitor 1 ( PAI 1 ) to serine proteinases , such as tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) , is mediated by the exosite interactions between the surface exposed variable region 1 , or 37 loop , of the proteinase and the distal reactive center loop ( RCL ) of PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The involvement of the urokinase type plasminogen activator ( uPA ) system in particular has been extensively studied in the pathogenesis of cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The degradation of basement membranes by tumor cells involves secretion and activation of proteinases , such as matrix metalloproteinases ( MMPs ) and the plasminogen activation system ( uPA , tPA , PAI 1 ) , and results from an imbalance between their inhibitors and activators , controlled by various growth factors or cytokines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have also demonstrated previously that transforming growth factor beta 1 ( TGF beta 1 ) stimulates urokinase type plasminogen activator ( uPA ) dependent invasion and metastasis of HRA cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators ( PAs ) , tissue PA ( tPA ) and urokinase PA ( uPA ) , have been shown to be induced in sensory neurons after sciatic nerve crush . ^^^ Both tPA and uPA activate some matrix metalloproteases ( MMPs ) , indirectly via plasminogen activation or directly , such as the uPA activation of MMP 2 . ^^^ In this study , we demonstrated , by using tPA and uPA knockout mice , that a lack of a plasminogen activator affected MMP 9 and MMP 2 activity after crushing of the sciatic nerve . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Liver regeneration and angiogenesis after partial hepatectomy were examined in mice lacking plasminogen or urokinase plasminogen activator ( uPA ) . ^^^ METHODS : Mice with a single gene deletion of plasminogen or uPA were subjected to 70 per cent partial hepatectomy . ^^^ RESULTS : The liver remnant weight was significantly reduced in mice lacking plasminogen or uPA compared with that in wild type mice on days 2 and 7 after partial hepatectomy . ^^^ CONCLUSION : Plasminogen and uPA are essential for optimal liver regeneration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased expression of the hepatocyte growth factor ( HGF ) receptor ( c met ) and urokinase type plasminogen ( uPA ) correlated with the development and metastasis of cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) system is central to a spectrum of biologic processes including fibrinoloysis , inflammation , atherosclerotic plaque formation , matrix remodeling during wound healing , tumor invasion , angiogenesis , and metastasis . ^^^ Binding of uPA with its receptor ( uPAR ) initiates a proteolytic cascade that results in the conversion of plasminogen to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Several new factors that may better describe tumour behaviour , like proliferation rate ( determined by thymidine labelling index , S phase fraction , mitotic index , or Ki 67 ) , presence of disseminated tumour cells , as well as expression of invasion factors ( urokinase type plasminogen activator uPA and its inhibitor PAI 1 ) and of cell cycle genes ( cyclin E ) , as well as gene expression patterns ( ' genomic profiling ' ) are currently discussed as future methods of risk assessment and also as tools for prediction of response to specific therapy modalities . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) were determined using ELISA in colorectal carcinoma WiDr , HT 29 and HT 29d cell lines with different metastatic potentials . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : We detected MMP 7 , urokinase plasminogen activator ( uPA ) and PAI 1 in both Matrigels , TIMP 2 was detected only in regular Matrigel and no MMP 1 or TIMP 1 was detected in either matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Molecular research into the invasiveness of HCC identified some factors positively related to invasiveness , P 16 and P 53 mutation , H ras , c cerbB 2 , mdm 2 , transforming growth factor ( TGF ) , epidermal growth factor receptor ( EGF R ) , matrix metalloproteinase 2 ( MMP 2 ) , urokinasetype plasminogen activator ( uPA ) , its receptor ( uPA R ) and inhibitor ( PAI 1 ) , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular endothelial growth factor ( VEGF ) , platelet derived endothelial cell growth factor ( PD ECGF ) , and basic fibroblast growth factor ( bFGF ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This activity is not seen with urokinase type Plg activator ( uPA ) but is induced in Plg / mice , confirming that the effect is Plg independent . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Co culture with H . pylori increased expression of mRNAs encoding interleukin ( IL ) 8 , vascular endothelial growth factor ( VEGF ) , angiogenin , urokinase type plasminogen activator ( uPA ) , and metalloproteinase ( MMP ) 9 by gastric carcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : We have previously reported that urinary urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) are elevated in patients with bladder cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To measure the urokinase type plasminogen activator ( UPA ) and its receptor ( UPAR ) in the plasma of patients with cerebral infarction and to study the effects of UPA and UPAR on cerebral infarction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To determine the contribution of urokinase type plasminogen activator ( uPA ) and plasmin in the invasion of highly invasive urothelial cancer cells . ^^^ RESULTS : Expression levels of mRNA , protein , and activities of uPA were significantly higher ( p < 0 . 005 ) and resulted in more plasminogen activation in UCT 2 than in UCT 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have further demonstrated that VEGF stimulated the expression of PAI 1 , but not urokinase plasminogen activator ( uPA ) , in keloid fibroblasts at both mRNA and protein levels , in a dose and time dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The peritoneal fluid of women with endometriosis contains an increased insulin like growth factor 1 ( IGF 1 ) bioavailability , which is produced by limited hydrolysis of urokinase type plasminogen activator ( uPA ) on IGF binding protein 3 ( IGFBP 3 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Moreover , in DU 145 AR plasminogen activator ( PA ) activity and secreted urokinase type plasminogen activator ( uPA ) protein were lower than in AR negative cells ; again testosterone induced PA activity and uPA protein only in DU 145 AR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Such a hybrid protein was synthesized consisting of the noninternalizing amino terminal fragment ( ATF ) of urokinase type plasminogen activator ( uPA ) for binding , and the catalytic portion of diphtheria toxin ( DT ) for killing , and the translocation enhancing region ( TER ) of DT for internalization . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) , its cell bound receptor ( uPAR ) and its main inhibitor plasminogen activator type 1 ( PAI 1 ) are present primarily in stromal cells in invasive breast carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the last few months , there have been three sets of new , provocative data that might have important implications for the daily prescription of adjuvant chemotherapy ( CT ) in the future . ( 1 ) Urokinase type plasminogen activator ( UPA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) , two molecular markers of invasion already known for their powerful prognostic value in node negative breast cancer , seem to predict for enhanced benefit from adjuvant CT , while the benefit from adjuvant endocrine therapy seems independent of them . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Levels of phenotypic markers ( alkaline phosphatase activity , osteocalcin release ) , and urokinase plasminogen activator ( uPA ) and insulin like growth factor 1 ( IGF 1 ) levels , were evaluated in each group . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The urokinase mediated plasminogen activation ( uPA ) system plays a central role in a number of cellular processes including tissue remodeling , cell migration , and angiogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The various mediators in the extracellular matrix ( matrix metalloproteinases 1 [ MMP 1 transforming growth factor beta [ TGF beta ] , urokinase plasminogen activator [ uPA ] , and type 1 collagen ) tended to show the highest expression with Vypro 2 ( 13 . 6 , 113 . 2 , 132 . 7 , and 139 . 5 , respectively ) and the lowest expression with Ti Mesh ( 11 . 9 , 68 . 5 , 92 . 8 , and 75 . 0 , respectively ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Renal gene expressions of type 1 plasminogen activator inhibitor ( PAI 1 ) , tissue type PA ( tPA ) , and urokinase type PA ( uPA ) were examined by real time PCR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition to inhibiting the anticoagulant protein C pathway , PCI also inhibits urinary plasminogen activator ( uPA ) , which is a well known mediator of tumor cell invasion . ^^^ Since PCI itself did not affect the proliferation rate of Caki 1 cells or cell expression of uPA in vitro , the effect of uPA , PCI , heat inactivated PCI and plasminogen activator inhibitor ( PAI ) 1 on the invasive potential of cultured RCC cells was evaluated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In bone , this repair attempt modifies insulin like growth factor 1 ( IGF 1 ) , IGF binding proteins ( IGFBPs ) , and transforming growth factor beta ( TGF beta ) , and alters the urokinase plasminogen activator ( uPA ) / plasmin system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have recently reported that tyrosine kinase , p 56 ( lck ) regulates cell motility and nuclear factor kappaB mediated secretion of urokinase type plasminogen activator ( uPA ) through tyrosine phosphorylation of IkappaBalpha following hypoxia / reoxygenation ( Mahabeleshwar , G . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have recently reported that osteopontin ( OPN ) stimulates cell motility and nuclear factor kappaB mediated secretion of urokinase type plasminogen activator ( uPA ) through phosphatidylinositol 3 kinase / Akt signaling pathways in breast cancer cells ( Das , R . , Mahabeleshwar , G . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The preparation and assessment of biological activity of 6 substituted 2 naphthamidine inhibitors of the serine protease urokinase plasminogen activator ( uPA , or urokinase ) is described . 2 Naphthamidine was chosen as a starting point based on synthetic considerations and on modeling of substituent vectors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Most LPA ( 2 ) TG ovaries produced significantly higher levels than non TG ovaries of type A , but not type B , vascular endothelial growth factor ( VEGF ) , isomers of VEGF A , and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The authors recently demonstrated that Ganoderma lucidum inhibits constitutively active transcription factors nuclear factor kappa B ( NF kappaB ) and AP 1 , which resulted in the inhibition of expression of urokinase type plasminogen activator ( uPA ) and its receptor uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In acute inflammatory condition , little is known about the expression of the urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) in the gastric fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this study was to determine the presence of angiostatin in ascitic and pleural effusions from cancer patients , as well as of metalloproteinases ( MMPs ) and urokinase type plasminogen activator ( uPA ) , both involved in angiostatin generation in in vitro models . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Matrix metalloproteinase ( MMP ) , plasmin , and urokinase type plasminogen activator ( uPA ) activities were determined by zymography and fluorogenic assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) expression in breast cancer is associated with relapse and a reduction in disease specific survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have identified RHAU , a putative DExH RNA helicase that was isolated in association with the ARE of urokinase plasminogen activator mRNA ( ARE ( uPA ) ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This system comprises of , among others , the urokinase type plasminogen activator ( uPA ) and its main inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) degrades the extracellular matrix and plays critical roles in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator ( uPA ) is implicated in pericellular proteolysis in a variety of physiological and pathological processes including angiogenesis and tumor metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We found the induction of mRNAs for tissue type plasminogen activator ( tPA ) and urokinase plasminogen activator ( uPA ) in the rat dorsal root ganglia following sciatic nerve transection . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Therefore , the expression of plasminogen , matrix metalloproteinases ( MMP 2 and MMP 9 ) , and of the serine protease urokinase type plasminogen activator ( uPA ) in serous epithelial carcinoma of the ovary were investigated . ^^^ In the same tissue extracts , semiquantitative estimates of MMP 2 , MMP 9 , total MMP activity , and uPA activity were determined using semiquantitative gelatin zymography in the presence or absence of human plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) system consists of the serine protease uPA , its glycolipid anchored receptor , uPAR and its 2 serpin inhibitors , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin , the pivotal thrombolytic enzyme , is generated on the surface of many cell types , where urokinase receptor ( uPAR ) bound urokinase ( uPA ) activates cell bound plasminogen ( Plg ) . ^^^ Phorbol 12 myristate 13 acetate ( PMA ) stimulated but not resting neutrophils dissolved fibrin clots , and this activity was not only uPA and Plg dependent but also alpha ( M ) beta ( 2 ) dependent . ^^^ Purified alpha ( M ) beta ( 2 ) directly bound uPA ( K ( d ) = 40 nm ) and Plg ( K ( d ) = 1 microm ) in a dose dependent and saturable manner . ^^^ In Plg activation assays , addition of purified alpha ( M ) beta ( 2 ) , but not a control protein , to a single chain uPA ( sc uPA ) / Plg mixture , decreased the K ( m ) from 2 to 0 . 1 microm , thereby augmenting the overall reaction efficiency by 50 fold . ^^^ When Plg was added to PMA stimulated neutrophils , both uPA and Plg were co immunoprecipitated with alpha ( M ) beta ( 2 . ) Thus , assembly of Plg and uPA on integrin alpha ( M ) beta ( 2 ) regulates Plm activity and , thereby , plays a crucial role in neutrophil mediated thrombolysis . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
H . polymorpha ret 1 mutants , which expressed truncated alpha COP lacking more than 300 C terminal amino acids , manifested an enhanced ability to secrete human urokinase type plasminogen activator ( uPA ) and an inability to grow with a shortage of Ca2+ ions , whereas a lack of alpha COP expression was lethal . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHOD : We studied urokinase ( uPA ) , tissue type plasminogen activator ( tPA ) , urokinase receptor ( uPAR ) and plasminogen activator inhibitor 1 ( PAI 1 ) expression by in situ hybridization and by immunohistochemistry in 14 NF 2 and 15 sporadic patients with 34 schwannomas . uPAR and vitronectin immunohistochemistry were also studied . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Among the candidate stimulating factors tested , tumor necrosis factor ( TNF ) alpha and IL 1beta ( to a lesser extent ) were found to drastically increase urokinase type plasminogen activator ( uPA ) and matrix metalloproteinase ( MMP ) 9 activities secreted from HOSE cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of the urokinase plasminogen activator ( uPA ) increases during the progression of colorectal tumors from adenomas to carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Collagen type 4 and hemoglobin were measured by Western blot analysis , matrix metalloproteinase ( MMP ) 2 and MMP 9 by gelatin zymography , and urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) by plasminogen casein zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and such plasminogen activation system components as uPA , PAI 1 and tPA were determined by enzyme immunoassay methods in endometrial tumors from 121 patients and 18 samples of endometrial hyperplasia of varying degree . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVES : To measure the plasma levels of urokinase plasminogen activator ( uPA ) , urokinase plasminogen activator receptor ( uPAR ) and plasminogen activator inhibitor 1 ( PAI 1 ) , and study the relationship between the plasma levels of uPA , PAI 1 and the serum albumin ( Alb ) , collagen type 4 ( CIV ) , the serum hyaluronic acid ( HA ) , prothrombin time ( PT ) and prothrombin activity ( PTA ) in patients with different stages of liver cirrhosis following chronic hepatitis B . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously demonstrated elevated levels of the fibrinolytic factors urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor ( PAI 1 ) in endometriotic tissue and endometrium from women with endometriosis . ^^^ The aim of the present study was to localize the uPA , PAI 1 and urokinase plasminogen activator receptor ( uPAR ) mRNA in endometriotic tissue and in endometrium both from women with and without endometriosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To probe into the expression and clinical significance of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) in urinary transitional cell carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In recent decades , evidence has been accumulating showing the important role of urokinase type plasminogen activator ( uPA ) in growth , invasion , and metastasis of malignant tumours . ^^^ It therefore initially came as a surprise that a high tumour level of the uPA inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) is also associated with a poor prognosis , the PAI 1 level in fact being one of the most informative biochemical prognostic markers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 : novel tumor derived factors with a high prognostic and predictive impact in breast cancer . ^^^ Urokinase type plasminogen activator ( uPA ) and its inhibitor , PAI 1 , play a key role in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The complex between urokinase type plasminogen activator ( uPA ) and its type 1 inhibitor ( PAI 1 ) independently predicts response to first line endocrine therapy in advanced breast cancer . ^^^ It has been shown that urokinase type plasminogen activator ( uPA ) and its main inhibitor ( PAI 1 ) have predictive value for therapy success in advanced breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this review we describe the mechanisms that underlie Ras regulation of vascular endothelial growth factor ( VEGF ) , cyclooxygenases ( COX 1 / 2 ) , thrombospondins ( TSP 1 / 2 ) , urokinase plasminogen activator ( uPA ) and matrix metalloproteases 2 and 9 ( MMP 2 / 9 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( uPAR ) serves as a receptor for urokinase plasminogen activator ( uPA ) and plays a role in invasion and migration of certain immune cells , including NK cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied the activity of urokinase plasminogen activator ( uPA ) , its inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) , and 92 kDa gelatinase B ( matrix metalloproteinase 9 , MMP 9 ) , using protein gel and reversed gel zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
R ) , IGF binding protein 3 ( IGFBP 3 ) , urokinase type plasminogen activator ( uPA ) and uPA receptor ( uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To assess the participation of urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators in embryo development and implantation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using several different concentrations of amiloride , aminobenzamidine and PAI 1 and the urokinase type plasminogen activator ( uPA ) function blocking antibody ( 3689 ) , we showed that uPA , acting as a protease , is responsible for production of alpha6p . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of urokinase plasminogen activator by epigallocatechin 3 gallate in human fibrosarcoma cells . ( ) Epigallocatechin 3 gallate ( EGCG ) , a main flavanol of green tea , potently suppressed the urokinase type plasminogen activator ( uPA ) expression in human fibrosarcoma HT 1080 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stopped flow fluorometry was used to study the kinetics of the reactive center loop insertion occurring during the reaction of N ( ( 2 ( iodoacetoxy ) ethyl ) N methyl ) amino 7 nitrobenz 2 oxa 3 diazole ( NBD ) P 9 plasminogen activator inhibitor 1 ( PAI 1 ) with tissue ( tPA ) and urokinase ( uPA ) type plasminogen activators and human pancreatic elastase at pH 5 . 5 8 . 5 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To understand alterations to the urokinase system that may occur in progressively metastatic prostate cancer cells , we assessed urokinase plasminogen activator receptor ( uPAR ) expression , in vitro motility towards vitronectin , urokinase plasminogen activator ( uPA ) induced growth and growth factor regulation of uPAR expression in three cell lines PC 3 and two derivatives from secondary metastases , PC 3M and PC 3MM2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical staining with tissue derived plasminogen activator ( tPA ) , urokinase derived plasminogen activator ( uPA ) , plasminogen activator inhibitor ( PAI 1 ) and von Willebrand Factor ( vWF ) was performed and analyzed with bright field microscopy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously demonstrated that Ganoderma lucidum down regulated the expression of NF kappaB regulated urokinase plasminogen activator ( uPA ) and uPA receptor ( uPAR ) , which resulted in suppression of cell migration of highly invasive human breast and prostate cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Matrix metalloproteinase 13 ( MMP 13 ) , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) have been reported to be involved in aseptic loosening of artificial hip joints . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : In the present study , we investigated the association between tumor tissue levels of tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) and prognosis in patients with primary breast cancer and analyzed whether TIMP 1 may be useful as a prognostic marker in combination with urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interpleural effusates were devoid of plasmin activity or active plasminogen activator inhibitor 1 but contained plasmin complexes and active urokinase like plasminogen activator ( uPA ) , alpha ( 2 ) antiplasmin , and thrombin activatable fibrinolysis inhibitor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
XR 5967 , a diketopiperazine , dose dependently inhibited the activity of human and murine PAI 1 , towards urokinase plasminogen activator ( uPA ) , with IC 50 values of 800 nM and 8 . 3 microM , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cell lines were evaluated for : IGF 1R expression ; expression of IGF binding proteins ( IGFBPs ) ; effect of IGF 1 on tumor cell growth , invasion , expression of urokinase plasminogen activator ( uPA ) , and soluble uPA receptor ( suPAR ) , and ; ectopic and orthotopic tumorigenicity of the canine OS cells in athymic mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Application of in gel protease assay in a biological sample : characterization and identification of urokinase type plasminogen activator ( uPA ) in secreted proteins from a prostate cancer cell line PC 3 . ^^^ Mass spectrometry ( MS ) identified the protease as urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Human atherosclerotic lesions contain elevated levels of urokinase plasminogen activator ( uPA ) , expressed predominantly by macrophages . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) in gestational tissues ; Measurements and clinical implications . ^^^ BACKGROUND : Urokinase plasminogen activator ( uPA ) and urokinase plasminogen activator receptor ( uPAR ) are central molecules for uPA / uPAR / plasmin dependent proteolysis , which is thought to play a significant role in the development of pregnancy , as well as its many complications . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
D QSAR CoMFA / CoMSIA studies on Urokinase plasminogen activator ( uPA ) inhibitors : a strategic design in novel anticancer agents . ^^^ Comparative molecular field analysis ( CoMFA ) and comparative molecular similarity indices analysis ( CoMSIA ) was performed on a series of indole / benzoimidazole 5 carboxamidines as urokinase plasminogen activator ( uPA ) inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To observe the effect of urokinase type plasminogen activator ( uPA ) on the development of subepithelial haze after photorefractive keratectomy ( PRK ) and to assess pregnancy as a risk factor for haze . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) plays a critical role in malignancies , and its overexpression has been linked to poor clinical prognosis in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this letter we report the synthesis and biochemical evaluation of selective , irreversible diphenyl phosphonate inhibitors for urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) play an important role in extracellular matrix degradation and cell migration in the central nervous system ( CNS ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
VEGF 165 and VEGF E , both interacting with VEGFR 2 , but not PlGF exclusively stimulating VEGFR 1 , induce within minutes internalization of uPAR via an LDL receptor like molecule , dependent on generation of active uPA and the presence of plasminogen activator inhibitor 1 ( PAI 1 ) . uPAR seems to play a pivotal role in VEGFR 2 induced endothelial cell migration because cleavage of surface uPAR impaired the migratory response of endothelial cells toward VEGF E , but not toward PlGF . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The prognostic importance of urinase type plasminogen activators ( uPA ) and plasminogen activator inhibitors ( PAI 1 ) in the primary resection of oral squamous cell carcinoma ] . ^^^ This article examines the prognostic importance of urinase type plasminogen activators ( uPA ) and of plasminogen activator inhibitors ( PAI 1 ) in cases of primary oral squamous cell carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparison of urokinase type plasminogen activators ( uPA ) and plasminogen activator inhibitors ( PAI 1 ) in primary resection of oral squamous cell carcinoma ] . ^^^ This article examines the distribution and prognostic importance of urinase type plasminogen activators ( uPA ) and of plasminogen activator inhibitors ( PAI 1 ) in cases of primary oral squamous cell carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Several 8 substituted 2 naphthamidine based inhibitors of the serine protease urokinase plasminogen activator ( uPA ) are described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type ( uPA ) plasminogen activator is regulated by serine protease inhibitors ( serpins ) , especially plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : We investigated whether the increase of urokinase type plasminogen activator ( uPA ) monocyte expression and chemokine releases induced by oxidised low density lipoproteins ( LDL ) , which participate to vascular tissue remodeling and to atherosclerotic plaque rupture , involved proinflammatory phospholipid products having platelet activating factor ( PAF ) like activity via the PAF receptor pathway . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease urokinase type plasminogen activator ( uPA ) interacts with a specific receptor ( uPAR ) on the surface of various cell types , including tumor cells , and plays a crucial role in pericellular proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased expression of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) is associated with different pathological conditions . ^^^ To evaluate the involvement of plasminogen in such circumstances , we have taken advantage of transgenic mouse models in which overexpression of uPA and / or uPAR in enamel epithelium , basal epidermis , and hair follicles leads to a pathological phenotype ; uPA transgenic mice have chalky white incisors and , when uPAR is co expressed , develop extensive alopecia , epidermal thickening , and subepidermal blisters . ^^^ These results do not argue in favor of a role for uPAR mediated signaling in our experimental model ; rather , they demonstrate an essential , dose dependent , requirement for plasminogen in uPA mediated tissue alterations . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have synthesized a dual specificity fusion toxin DTU2GMCSF composed of the catalytic and translocation domains of diphtheria toxin ( DT ) fused to the granulocyte macrophage colony stimulating factor ( GM CSF ) in which the DT furin cleavage site 163RVRRSV170 is modified to a urokinase plasminogen activator ( uPA ) cleavage site 163GSGRSA170 , termed U 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Changes in gene expression were determined by RT PCR and quantified by real time PCR for urokinase plasminogen activator ( uPA ) , collagenase 1 ( MMP 1 ) , membrane type 1 metalloproteinase ( MT 1 MMP ) , gelatinases A and B ( MMP 2 , MMP 9 ) , tissue inhibitor 2 ( TIMP 2 ) , VEGF and its receptors , Flt 1 ( VEGFR 1 ) , KDR ( VEGFR 2 ) , and neuropilin 1 ( NP 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To clarify the role of proteins involved in the regulation of fibrinolysis during corneal angiogenesis , we have studied corneal vessel formation in mice deficient for urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen , plasminogen activator inhibitor 1 ( PAI 1 ) and thrombin activatable fibrinolysis inhibitor ( TAFI ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously reported in a series of papers that a Kunitz type protease inhibitor , bikunin , suppresses up regulation of urokinase type plasminogen activator ( uPA ) and its specific receptor ( uPAR ) expression , phosphorylation of ERK1 / 2 and cancer cell invasion in vitro and peritoneal disseminated metastasis in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect of TSP 1 on uPAR and its ligand , urokinase plasminogen activator ( uPA ) , expression were determined by ELISA . ^^^ To determine the role of uPAR on TSP 1 mediated KB tumor cell invasion , we used the three following different strategies : ( a ) . blocking uPAR or its ligand , uPA , with neutralizing antibodies ; ( b ) . enzymatic cleavage of uPAR with glycosylphosphatidylinositol ( GPI ) specific phospholipase C ; and ( c ) . inhibition of plasminogen binding by using epsilon aminocaproic acid . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , NF kappaB and AP 1 control the expression of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) , and expression of both uPA and uPAR correlates with invasive cancer cell phenotype and poor prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVES : To examine whether urokinase type plasminogen activator ( uPA ) and type 1 plasminogen inhibitor ( PAI 1 ) , DNA ploidy , and S phase fraction ( SPF ) add supplementary prognostic information relative to stage and Fuhrman ' s grade in renal cell carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND & OBJECTIVE : Urokinase type plasminogen activator ( uPA ) / its receptor ( uPAR ) , serine protease system , plays a key role in the degradation of extracellular matrix and basement membranes , and intensifying the tumor invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) has been implicated in neointima formation and arterial lumen narrowing after angioplasty . ^^^ To determine the specificity of the action of uPA on vessel remodelling after arterial injury we compared the effects of the recombinant urokinase and tissue type plasminogen activators on vessel morphology , cell migration and proliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 2 ( PAI 2 ) , an efficient and specific inhibitor of uPA , has been shown to selectively deliver potent cytotoxins to tumour cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In accord with the increased fibrinolytic activity , the levels of urokinase type plasminogen activator ( uPA ) in the medium were also increased . ^^^ However , there was no significant difference of plasminogen activator inhibitor 1 ( PAI 1 ) , pro uPA , and tissue type plasminogen activator ( tPA ) concentrations in the medium between dDAVP treatment and control . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TGF beta 1 also downregulated urokinase type plasminogen activator ( uPA ) activity while upregulating PA inhibitor ( PAI ) 1 and thrombospondin ( TSP ) 1 gene expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( UPA ) has been implicated in a broad spectrum of pathogenic processes involved in the formation and disruption of atherosclerotic lesions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One of its downstream target genes is urokinase plasminogen activator ( uPA ) , which is involved in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : The serine protease urokinase type plasminogen ( uPA ) and its receptor ( uPAR ) appear to have a major function in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of the urokinase type plasminogen activator ( uPA ) gene and the fibrinolytic activity were assessed by Northern blotting and fibrin zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) plays a role in vascular remodeling by facilitating smooth muscle cell migration and proliferation in addition to the proteolysis of extracellular matrix , and the expression of uPA is elevated in atherosclerotic lesions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The prognostic value of components of the urokinase type plasminogen activator ( uPA ) system , its receptor uPAR ( CD 87 ) , and plasminogen activator inhibitors PAI 1 and PAI 2 is well established . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators ( tPA and uPA ) are serine proteases that convert the circulating zymogen plasminogen to active plasmin and mediate fibrin degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) system consists of the serine protease uPA , the glycolipid anchored receptor , uPAR , and the 2 serpin inhibitors , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Differential localization and expression of urokinase plasminogen activator ( uPA ) , its receptor ( uPAR ) , and its inhibitor ( PAI 1 ) mRNA and protein in endometrial tissue during the menstrual cycle . ^^^ The urokinase plasminogen activator ( uPA ) dependent proteolytic cascade as well as ligand activation of the uPA receptor ( uPAR ) is critically involved in physiological as well as pathophysiological aspects of tissue expansion and remodelling . ^^^ Cyclic variation and distribution of uPA , uPAR and plasminogen activator inhibitor 1 ( PAI 1 ) mRNA were examined by in situ hybridization , real time PCR and northern blot in normal endometrium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore OPN enhanced NIK regulated urokinase type plasminogen activator ( uPA ) secretion , uPA dependent pro MMP 9 activation , cell motility , and tumor growth . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activation system includes the urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) , among other molecules . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Levels of urokinase plasminogen activator activity ( uPA ) measured using an amidolytic assay were raised after IPC . ^^^ However , enzyme linked immunosorbent assays ( ELISA ) of uPA antigen , and the activities of tissue plasminogen activator ( tPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were not statistically different from those in control experiments . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
LRP protein was reduced and extracellular accumulation of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) proteins were greater in uPAR / cultures . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma levels of fibrinopeptide A ( FPA ) , fibrin d dimer , thrombin antithrombin ( TAT ) complex , prothrombin fragment 1 . 2 ( F1 . 2 ) , urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were measured before and after therapy , as was the cellular expression of the genes for tissue factor ( TF ) and interleukin 1 beta ( IL 1 beta ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A method has been developed for accurately and precisely measuring the activity of a range of plasminogen activators ( PAs ) used as thrombolytic agents , including streptokinase , tissue plasminogen activator ( tPA ) and variants , and urokinase ( uPA ) , both single and two chain forms . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the major physiological inhibitor of both tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen system participates in this process with the balance between urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) being a critical determinant of the extent of collagen accumulation that follows lung injury . ^^^ Because the plasminogen system is known to influence the rate of migration of epithelial cells , including keratinocytes and bronchial epithelial cells , we hypothesized that the balance of uPA and PAI 1 would affect the efficiency of alveolar epithelial cell ( AEC ) wound repair . ^^^ The significant effect of uPA and PAI 1 on epithelial repair suggests a mechanism by which the plasminogen system may modulate pulmonary fibrosis . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human urokinase type plasminogen activator ( uPA ) is a serine protease that converts plasminogen to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Nevertheless , plasminogen binding in the presence of the urokinase type plasminogen activator ( uPA ) promoted the invasion of HeLa cells by M . fermentans . ^^^ Cholesterol depletion and sequestration with beta cyclodextrin and filipin , respectively , did not affect the capacity of M . fermentans to adhere , but invasion of HeLa cells by uPA activated plasminogen bound M . fermentans was impaired , suggesting that lipid rafts are implicated in M . fermentans entry . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the study reported here we examined the expression of plasminogen activator inhibitor 1 ( PAI 1 ) , urokinase type plasminogen activator ( uPA ) , and uPA receptor ( uPAR ) , as well as the relevance of such expression to the production of type 4 collagen , a major component of extracellular matrix , in the renal tissue of rats with streptozotocin induced diabetes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To investigate the expression and localization of urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) and their interaction with the actin cytoskeleton in human corneal fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The cell surface receptor ( uPAR ) for urokinase plasminogen activator ( uPA ) is a strong prognostic marker in several types of cancer . uPA cleaves the three domain protein uPAR ( 1 3 ) into two fragments : uPAR ( 1 ) , which contains domain 1 ; and uPAR ( 2 3 ) , which contains domains 2 and 3 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this study was to evaluate the expression of urokinase plasminogen activator ( uPA ) , its receptor ( uPAR ) and the plasminogen activator inhibitor type 1 ( PAI 1 ) in human aorta , and to balance them with the stage of atherosclerotic lesion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : We performed a retrospective study on the immunohistochemical expression of bikunin , urokinase type plasminogen activator ( uPA ) and macrophages ( CD 68 ) in surgical specimens derived from 89 ovarian cancer patients to investigate correlations between the expression of bikunin and the clinicopathologic features and the prognosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a positive regulator of outer root sheath keratinocyte proliferation . ^^^ Urokinase plasminogen activator ( uPA ) , a serine proteinase , is important in the development and epidermal wound healing , and seems to play a regulatory role in the proliferation of mouse epidermal keratinocytes ( KC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation of plasminogen by urokinase plasminogen activator ( uPA ) plays important roles in several physiologic and pathologic conditions . ^^^ In contrast , neither Glunor Lys plasminogen inhibits the activation of plasminogen by 2 chain uPA . ^^^ These studies provide insight into the regulation of uPA mediated plasminogen activation and identify a novel mechanism for its fibrin specificity . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The following parameters were determined : concentration of the urokinase plasminogen activator antigen ( uPA : Ag ) , plasmin antiplasmin complexes ( PAP ) , fibrin and fibrinogen degradation products ( FDP ) , activity of prekallikrein ( PK ) and C 1 inhibitor ( C 1 INH ) and also euglobulin clot lysis time ( ELT ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The release of urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI ) by GEC or whole glomeruli was assessed by ELISA , fibrin zymography and Northern blot . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the effect of nuclear factor kappaB ( NF kappaB ) decoy oligodeoxynucleotides ( ODN ) on NF kappaB activation and expressions of vascular endothelial growth factor ( VEGF ) , urokinase type plasminogen activator ( uPA ) , intercellular cell adhesion molecule 1 ( ICAM 1 ) of SKOV 3 cell . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Most interestingly , the occurrence of SB 203580 induced migratory inhibition coincided with a reduction of urokinase plasminogen activator ( uPA ) expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The net balance between urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) has been implicated in tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Because absence of urokinase plasminogen activator ( uPA ) prevents development of cardiac fibrosis after experimental myocardial infarction in mice , we hypothesized that elevated activity of uPA or deficiency of the uPA inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) might cause cardiac fibrosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) system plays a central role in the blood clot dissolution and tissue plasticity . uPA is a serine protease that is also involved in the metastatic process upon activation and binding to its receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have analyzed whether poly ( ADP ribose ) polymerase activity is involved in basic fibroblast growth factor ( FGF 2 ) mediated upregulation of urokinase type plasminogen activator ( uPA ) mRNA . ^^^ We demonstrated that specific inhibition of poly ( ADP ribose ) polymerase activity via 3 aminobenzamide ( 3ABA ) or NAD+ deprivation prevents FGF 2 mediated uPA mRNA over expression and cell associated plasminogen activator ( PA ) production in GM 7373 endothelial cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor levels of BCAR 1 were correlated with relapse free survival ( RFS ) and overall survival ( OS ) and compared with collected data on urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this study was to determine whether transforming growth factor beta 1 ( TGF beta 1 ) induces the synthesis , release and gene expression of urokinase type plasminogen activator ( uPA ) in hepatic stellate cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase ( uPA ) and tissue plasminogen activator ( tPA ) are serine proteases implicated in fibrinolysis , but their role in the regulation of the cerebrovascular response to brain trauma has not been investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using gel zymography for matrix metalloproteinase ( MMP ) detection and a colorimetric assay for urokinase type plasminogen activator ( uPA ) analysis , we analyzed the proteinase activities of MMP 2 , MMP 9 , and uPA from the prepared vesicles , whole cells isolated from ascites , and the cell free ultracentrifuged supernatant . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present study was undertaken to identify possible differences between normal and SSc microvascular endothelial cells ( MVECs ) in the expression of the cell associated urokinase type plasminogen activator ( uPA ) / uPA receptor ( uPAR ) system , which is critical in the angiogenic process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND / PURPOSE : Urokinase plasminogen activator ( uPA ) is a serine proteinase that has been suggested to play an important role in tumor invasion and metastasis . ^^^ It binds to a specific membrane receptor , uPA receptor ( uPAR ) , and activates plasminogen to form plasmin , which participates in tissue degradation and proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It has been proposed that urokinase type plasminogen activator ( uPA ) , under the control of growth factors or cytokines , provides the proteolytic potential to the accomplishment of these cellular events . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cellular invasion ( Matrigel invasion ) , migration ( colloidal gold ) , and urinary type plasminogen activator ( uPA ) activity ( colorimetric assay ) were quantified . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here we demonstrate that genistein suppresses cell adhesion and migration by inhibiting the constitutively active transcription factors NF kappaB and AP 1 , resulting in the suppression of secretion of urokinase type plasminogen activator ( uPA ) in breast cancer cells MDA MB 231 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We previously found that bikunin ( bik ) , a Kunitz type protease inhibitor , suppresses transforming growth factor beta 1 ( TGF beta 1 ) stimulated expression of urokinase type plasminogen activator ( uPA ) in human ovarian cancer cells that lack endogenous bik . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Semi quantitative analysis was used to observe stained intensity and area of tissue type plasminogen activator , urokinas type plasminogen activator / plasminogen activator inhibitor ( tPA , uPA / PAI 1 ) in remnant renal tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation is believed to be critical to the progression of oral squamous cell carcinoma by facilitating matrix degradation during invasion and metastasis , and high levels of urokinase plasminogen activator ( uPA ) and plasminogen activator ( PA ) inhibitor 1 ( PAI 1 ) in tumors predict poor disease outcome . ^^^ Laser capture microdissection ( LCM ) was combined with plasminogen casein zymography to analyze uPA , tissue PA ( tPA ) , uPA PAI 1 complexes , and tPA PAI 1 complexes in 11 tumors and adjacent non malignant epithelium from squamous cell carcinomas of the tongue , floor of mouth , larynx , and vocal cord . uPA was detectable in all tumor samples analyzed , uPA PAI 1 complexes in three samples , and tPA in nine . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Specifically , overexpression of SRF in human lung fibroblasts upregulated urokinase type plasminogen activator ( uPA ) and its substrate Plg , whereas it downregulated plasminogen activator inhibitor ( PAI ) 1 . ^^^ Because uPA cleaves Plg into plasmin , which activates matrix metalloproteinases ( MMP ) , the end result was an increase in MMP activity . ^^^ To determine whether uPA , Plg , and PAI 1 were abnormally expressed in LAM in vivo , we immunostained 12 LAM cases . ^^^ In all cases , the LAM lesions showed stronger immunoreaction for uPA and Plg than the surrounding normal lung parenchyma . ^^^ Microdissection based reverse transcriptase / polymerase chain reaction further confirmed upregulation of uPA and Plg and downregulation of PAI 1 message in LAM . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have studied the expression of urokinase type plasminogen activator ( uPA ) mRNA , uPA receptor ( uPAR ) mRNA and immunoreactivity , and type 1 plasminogen activator inhibitor ( PAI 1 ) mRNA and immunoreactivity in 16 prostate adenocarcinomas and 9 benign prostate hyperplasias . uPA mRNA and uPAR mRNA expression were found in 9 and 8 of the adenocarcinomas , respectively , and in 7 and 6 of the benign hyperplasias , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHOD : In a consecutive series of 127 patients resected for primary squamous cell carcinoma of human larynx , the expression of uPA and plasminogen activator inhibitors ( PAI ) type 1 , type 2 was determined immunohistochemically . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A site directed mutant of the serine protease urokinase type plasminogen activator ( uPA ) , was produced to assess the contribution of the Ser 190 side chain to the affinity and selectivity of lead uPA inhibitors in the absence of other differences present in comparisons of natural proteases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RT PCR assay for urokinase type plasminogen activator ( uPA ) , its cellular receptor ( uPAR ) , and tumor necrosis factor ( TNF ) alpha was performed to assess the cecal tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Overexpression of urinary plasminogen activator ( uPA ) protein and mRNA in thyroid carcinogenesis . ^^^ OBJECTIVE : Urinary plasminogen activator ( uPA ) , a protease , is one of the critical components of tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By using immunohistochemical techniques , we studied the expression of matrix metalloproteinase ( MMP ) 1 , MMP 2 , MMP 9 , cathepsin B ( CatB ) , and urokinase plasminogen activator ( uPA ) in 29 NSBCs and compared these data with clinicopathologic parameters and the expression of cell differentiation markers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since stimulated PMNs express urokinase type plasminogen activator ( uPA ) , we sought to investigate the relevance of apo ( a ) fragmentation on plasminogen activation by neutrophils . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We previously demonstrated the doxorubicin induced urokinase type plasminogen activator ( uPA ) expression in human RC K 8 lymphoma cells and NCI H 69 small cell lung carcinoma cells in which reactive oxygen species might be involved . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both FGF 2 and phorbol ester inducible urokinase type plasminogen activator ( uPA ) expression requires AP 1 binding to an enhancer element in the uPA promoter , and we have previously shown that FGF 2 or PMA induction of uPA expression is strongly dependent on MEKK 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The main components in plasminogen activation include plasminogen , tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , urokinase plasminogen activator receptor ( uPAR ) , and plasminogen activator inhibitors 1 and 2 ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stable murine macrophage ( Raw264 . 7 ) cell lines expressing high levels of human uPAR , human urokinase plasminogen activator ( uPA ) , or both were established using expression vectors driven by the human CD 68 promoter . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Active plasmin is generated by proteolytic activation of the zymogen plasminogen ( Plg ) by urokinase type plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) play important physiological functions in extracellular proteolysis , as well as cell adhesion and migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A series of non amide linked 6 substituted 2 naphthamidine urokinase plasminogen activator ( uPA ) inhibitors are described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Combined determination of urokinase type plasminogen activator ( uPA ) and its inhibitor , activator inhibitor type 1 ( PAI 1 ) , supports risk adapted individualized therapy concepts , particularly in node negative breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVES : To determine the role played by the urokinase plasminogen activator ( uPA ) and urokinase plasminogen activator receptor ( uPAR ) system in choroidal neovascularization ( CNV ) and whether inhibition of this system can suppress the extent of CNV in an animal model . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Traditionally , urokinase type plasminogen activator ( uPA ) has been associated with pericellular proteolytic activity involved in tissue remodelling processes , and tissue type plasminogen activator ( tPA ) mainly with intravascular fibrinolysis . ^^^ METHODS : The expression of uPA , tPA , urokinase receptor ( uPAR ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and vitronectin was investigated by immunohistochemical staining , in addition to uPA , tPA and PAI 1 expression by in situ hybridization , in samples from eight chronic venous ulcers , five decubitus ulcers , five well granulating acute wounds and five normal skin samples . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , proteolytic activities of matrix proteinases such as urokinase type plasminogen activator ( uPA ) and matrix metalloproteinases ( MMPs ) are regulated by Ras isoforms . ^^^ As expected , the reduction of uPA and tPA activities by SCH 66336 inhibited the conversion of plasminogen to plasmin by about 25 % in PC 3 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Importantly , in a screening for genes induced after drug treatment we found that urokinase plasminogen type activator ( uPA ) was strongly regulated by cocaine in several protocols of drug administration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At birth , when fragmentation occurred , epithelial cells displayed an upregulation of membrane type 1 matrix metalloproteinase ( MT 1 MMP ) and urokinase type plasminogen activator ( uPA ) , as well as laminin alpha 1 mRNAs . ^^^ In the days following birth , gelatin and plasminogen casein zymography showed an increased enzymatic activity of MMP 2 and uPA , respectively . ^^^ Altogether , our data show that during follicle histogenesis , ovigerous cord fragmentation results from an acute BM component deposition in deepening clefts and that BM homeostasy involves proteinases of the MMP2 / MT1 MMP / TIMP3 and plasminogen / uPA families . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator ( uPA ) , a protease only expressed in highly invasive human breast cancer cells , is inhibited by DNA methylation of its promoter . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Currently , two transgenic lines that eat less are available , thus implicating the transgenic enzyme , i . e . the urokinase type plasminogen activator ( uPA ) , in causing the reduced appetite . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Scar and remaining viable LV myocardium ( LVM ) were separately analyzed for MMP 2 , 7 , 9 , urokinase type and tissue type plasminogen activator ( uPA and tPA ) mRNA levels by RT PCR . ^^^ MMP and plasminogen activators ( uPA , tPA ) mRNAs were increased accordingly . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The phenomenon in which urokinase type plasminogen activator ( uPA ) is induced in the axotomized facial nucleus suggests an interaction between injured motoneurons and microglia . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND / AIMS : Transplantation of primary human hepatocytes and establishment of hepatitis B virus ( HBV ) infection in immunodeficient urokinase plasminogen activator ( uPA ) transgenic mice was shown . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
CPL also down regulated the expression of urokinase type plasminogen activator ( uPA ) , one of key proteins associated with invasion and metastasis of tumor cells in a concentration dependent fashion , while CPL did n ' t significantly affect the expression of matrix metalloproteinase 2 ( MMP 2 ) and tissue inhibitor of metalloproteinase 2 ( TIMP 2 ) by reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study we investigated the relationship between patient outcome and levels of VEGF , urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in tumor cytosols of 196 node negative primary invasive breast cancer patients who did not receive any adjuvant therapy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition to its role as an inhibitor of urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) is hypothesized to regulate epithelial cell adhesion and migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Lysophosphatidic acid ( LPA ) enhances urokinase plasminogen activator ( uPA ) expression in ovarian cancer cells ; however , the molecular mechanisms responsible for this event have not been investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In a chromogenic substrate based urokinse ( uPA ) / PAI 1 assay and a tissue type plasminogen activator ( tPA ) mediated clot lysis assay , ZK 4044 inhibited human PAI 1 activity with IC 50 values of 644+ / 255 and 100+ / 90 nM , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Introduction of a TRAF 2 expression vector in CD 95 sensitive Colo 357 cells resulted in ( 1 ) resistance to CD 95 induced apoptosis ; ( 2 ) increased constitutive NF kappaB and AP 1 activity ; and ( 3 ) higher basal secretion of matrix metalloproteinases ( MMPs ) , urokinase type plasminogen activator ( uPA ) , and IL 8 , leading to increased invasiveness . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The high affinity interaction between the urokinase type plasminogen activator ( uPA ) and its glycolipid anchored cellular receptor ( uPAR ) promotes plasminogen activation and the efficient generation of pericellular proteolytic activity . ^^^ Pericellular plasminogen activation was reduced by approximately 50 fold in the presence of CD 82 , although levels of components of the plasminogen activation system were unchanged . uPAR was present on the cell surface and molecularly intact , but radioligand binding analysis with uPA and anti uPAR antibodies revealed that it was in a previously undetected cryptic form unable to bind uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Of the various p 38 isoforms , p38alpha has been shown to mediate the in vitro invasiveness of breast cancer cells through up regulation of urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) gene expression was greatly up regulated by increasing the concentration of polysaccharides . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND AND AIMS : Overexpression of urokinase type plasminogen activator ( uPA ) has been shown to be strongly associated with an increased metastatic potential and poor prognosis in a variety of human malignancies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) have been suggested to play an important role in inflammatory diseases . ^^^ Increased levels of tPA , uPA , uPA receptor ( uPAR ) , and their inhibitor , plasminogen activator inhibitor ( PAI ) 1 , have been found in the cerebrospinal fluid ( CSF ) of patients with bacterial meningitis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its inhibitor new prognostic factors in oral squamous cell carcinoma ] . ^^^ Several cell biological studies have shown that the invasiveness of different malignant tumors ( breast , renal , prostate , gastric , ovarian cancers ) depends at least in part on the urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 . uPA converts plasminogen into plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
EGFR phosphorylation and beta catenin activation were inhibited by plasminogen activator inhibitor 1 and pertussis toxin , two inhibitors of the urokinase type plasminogen activator ( uPA ) / uPA receptor system . beta Catenin activation was correlated with the phosphorylation of glycogen synthase kinase 3beta through a phosphatidylinositol 3 kinase / Akt dependent mechanism . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Nevertheless , tryptase epsilon readily converted single chain pro urokinase type plasminogen activator ( pro uPA / scuPA ) into its mature , enzymatically active protease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purpose of this study was to assess the expression of urokinase type ( uPA ) and tissue type ( tPA ) plasminogen activators in in vivo and in vitro preimplantation development in rat embryos using immunofluorescence confocal microscopy and computerized image analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the current study , we describe an optical zymographic procedure that specifically detects urokinase type plasminogen activator ( uPA ) activity in biological samples . ^^^ The procedure is independent of plasminogen and , therefore , can efficiently distinguish the active two chain uPA from its proenzyme . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Specifically , mice deficient in either urokinase type plasminogen activator ( uPA / mice ) or plasminogen demonstrate markedly impaired muscle regeneration after injury . ^^^ In the present study , we tested the hypothesis that loss of the primary inhibitor of uPA , plasminogen activator inhibitor 1 ( PAI 1 ) , would improve muscle regeneration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
LysoPC also induced the mRNA expression of urokinase type plasminogen activator ( uPA ) , uPA receptor , and plasminogen activator inhibitor 1 , but the kinetics were different from that of tPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) receptor ( uPAR ) functions in concert with co receptors , including integrins , FPR like receptor 1 / lipoxin A 4 receptor , and the epidermal growth factor receptor ( EGFR ) , to initiate cell signaling . uPAR co receptors may be dynamically organized into a multiprotein signaling receptor complex . ^^^ In two cell culture models of uPA stimulated breast cancer growth , MDA MB 468 cells treated with uPA and MCF 7 cells treated with uPA plasminogen activator inhibitor 1 complex , proliferation was completely inhibited when EGFR expression or activity was blocked . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RT PCR assay for urokinase type plasminogen activator ( uPA ) , its cellular receptor ( uPAR ) , tissue type plasminogen activator ( tPA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and tumor necrosis factor ( TNF ) alpha was performed to assess the cecal tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Considering their well documented mechanosensitivity , functional inter dependence , and involvement in angiogenesis , we hypothesized roles for matrix metalloproteinases ( MMP 2 / 9 ) , RGD dependent integrins , and urokinase plasminogen activator ( uPA ) in this process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we first examined the effect of mitogen activated protein kinase kinase ( MEK ) inhibitors on the production of matrix metalloproteinases ( MMPs ) , urokinase type plasminogen activator ( uPA ) , and tissue inhibitors of metalloproteinases ( TIMPs ) in a human gallbladder cancer cell line , NOZ cells in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NFkappaB p 50 DNA binding correlated positively with several prognostic biomarkers ; however , only NFkappaB p 50 DNA binding ( p=0 . 04 ) , Activator Protein 1 DNA binding ( AP 1 ; p < or=0 . 01 ) and urokinase type plasminogen activator expression ( uPA ; p=0 . 0014 ) showed significant associations with metastatic relapse and disease free patient survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here we have used the autoimmune collagen type 2 ( CII ) induced arthritis ( CIA ) model and mice deficient for urokinase type PA ( uPA ) or plasminogen to investigate the role of the PA system for development of arthritis . ^^^ Our finding that infiltration of inflammatory cells into the synovial joints was impaired in plasminogen deficient mice suggests that uPA and plasminogen are important mediators of joint inflammation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The involvements of matrix metalloproteinase 9 ( MMP 9 ) and urokinase plasminogen activator ( uPA ) in the pathogenesis of various types of lung cancers have been established . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical SABC methods were used to detect transplanted BMSCs and expression of urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OPN also enhanced JNK 1 dependent / independent AP 1 mediated urokinase type plasminogen activator ( uPA ) secretion , uPA dependent promatrix metalloproteinase 9 ( MMP 9 ) activation , cell motility , and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In situ hybridization revealed the expression of Insulin like growth factor 1 ( IGF 1 ) and urokinase type plasminogen activator ( uPA ) mRNA , while immunohistochemistry detected differential expression of uPA and cathepsin D . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enhancement of urokinase type plasminogen activator ( uPA ) secretion , but not that of substrate plasminogen ( PGn ) , by rat microglia stimulated with neuronal conditioned medium . ^^^ Assuming the presence of intercellular interactions between injured motoneurons and microglia in the axotomized facial nucleus , we investigated the effects of neuronal conditioned medium ( NCM ) on the release of urokinase type plasminogen activator ( uPA ) from microglia . ^^^ In contrast , the secretion of plasminogen ( PGn ) , a substrate of uPA , was not promoted by the NCM treatment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cathepsin B and pro urokinase plasminogen activator ( pro uPA ) localize to the caveolae of HCT 116 human colorectal carcinoma cells , an association mediated by active K RAS . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To study the correlation between expression of urokinase type plasminogen activator ( uPA ) and capability of tumor cell seeding to the peritoneal membrane by different gastric cancer lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously found that tissue type and urokinase type plasminogen activators ( tPA and uPA ) are induced in dorsal root ganglia ( DRG ) neurons after peripheral axotomy and that tPA plays crucial roles in generating neuropathic pain . ^^^ Here we examined whether the plasminogen activator inhibitor 1 and 2 ( PAI 1 and PAI 2 ) mRNA , endogenous inhibitors of tPA and uPA , are induced in the DRG following sciatic nerve transection . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of the urokinase type plasminogen activator ( uPA ) by hepatocyte growth factor / scatter factor ( HGF / SF ) plays an important role in tumor cell invasion and metastasis that is mediated through the Met receptor tyrosine kinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The authors found previously that plasma levels of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) were elevated in patients with bladder carcinoma and were associated with features of biologically aggressive disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Homozygous urokinase plasminogen activator ( uPA ) SCID mice underwent transplantation with primary human hepatocytes , and at different times animals were bled and sacrificed to analyze plasma and liver tissue , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator , uPa receptor , and its inhibitor in vernal keratoconjunctivitis . ^^^ Levels of plasminogen activity , uPA , tissue plasminogen activator ( tPA ) , and their inhibitor , plasminogen activator inhibitor type 1 ( PAI 1 ) were measured in tears and plasma of patients with VKC . ^^^ RESULTS : Tear levels of uPA and tPA and tear plasminogen activity levels were significantly greater in patients with VKC than in control subjects . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Tissue type plasminogen activator deficient ( tPA ( / ) ) , urokinase type plasminogen activator deficient ( uPA ( / ) ) , plasminogen activator inhibitor 1 deficient ( PAI 1 ( / ) ) , alpha 2 antiplasmin deficient ( alpha 2 AP ( / ) ) mice , and their wild type counterparts were used . ^^^ CONCLUSIONS : The results strongly suggest that endogenous tPA , but not uPA acts as a facilitator in NMDA induced retinal cell damage , and that its mechanism may not be associated with cleavage of plasminogen into plasmin in the fibrinolytic cascade . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although MEK kinase 1 ( MEKK 1 ) has been shown to regulate cell migration and urokinase plasminogen activator ( uPA ) expression , the precise role of MEKK 1 in this process remains unknown . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
QPCR analysis reveals that the genes for urokinase type plasminogen activator ( uPA ) , VEGF , and Pim 1 oncogene , known to promote tumor growth , were significantly downregulated , whereas IFN gamma , known to be a tumor suppressor gene , was significantly upregulated in f PSA treated PC 3M cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It is known that an increase in cell migration and invasion can be brought about by cytoskeletal alterations and basement membrane degradation due to increased expression of pro urokinase plasminogen activator ( pro uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Gangliosides inhibit urokinase type plasminogen activator ( uPA ) dependent squamous carcinoma cell migration by preventing uPA receptor / alphabeta integrin / epidermal growth factor receptor interactions . ^^^ The interaction of the urokinase type plasminogen activator ( uPA ) receptor ( uPAR ) with integrins plays a critical role in the regulation of cell adhesion and migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate whether separated and cultured endometriotic and endometrial stromal and epithelial cells release urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and soluble plasminogen activator receptor ( suPAR ) antigens in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation of the fibrinolytic system is dependent on the conversion of the plasma zymogen , plasminogen ( Pg ) , to the serine protease plasmin ( Pm ) by the physiological activators urokinase type Pg activator ( uPA ) or tissue type plasminogen activator ( tPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thus , the generation of plasmin involves the pro enzyme plasminogen , the urokinase type plasminogen activator uPA and its pro enzyme pro uPA , the uPA inhibitor PAI 1 , the cell surface uPA receptor uPAR , and the plasmin inhibitor alpha ( 2 ) antiplasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
E LDL in contrast to n LDL induced substantial activation of the plasminogen activation system as well as of the MMP system in monocytic cells , as measured by enhanced cell surface expression of the urokinase receptor ( uPAR ) , the extracellular matrix metalloproteinase Inducer ( EMMPRIN ) and the membrane type 1 MMPs ( MT 1 MMP , MMP 14 ) , as well as by secretion of active uPA , and of MMP 9 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine protease uPA ( urokinase type plasminogen activator ) and its receptor uPAR ( CD 87 ) are often elevated in malignant tumours , hence , inhibition of this tumour associated plasminogen activation system provides an attractive target for therapeutic strategies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Similar expression patterns were observed for urokinase plasminogen activator ( uPA ) , uPA receptor , and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the April ( 2005 ) issue of Cancer Biology & Therapy , Amir et al report on the novel role maspin plays in the regulation of the urokinase type plasminogen activator ( uPA ) and receptor ( uPAR ) protein system as it relates to hypoxia thereby illustrating yet another potential therapeutic pathway involving maspin . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To determine whether insulinlike growth factor 1 ( IGF 1 ) and hepatocyte growth factor ( HGF ) cooperate to induce migration and invasion of human colorectal carcinoma ( CRC ) cells and whether the effects of IGF 1 and / or HGF are mediated through activation of the urokinase plasminogen activator ( uPA ) / uPA receptor ( uPAR ) system , a central mediator of tumor cell migration and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Subsequently , we examined whether SPC affected the production of urokinase type plasminogen activator ( uPA ) , an important regulator of angiogenesis , and found that SPC stimulated the expression of uPA at both the transcriptional and translational levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The structure was solved at 2 . 7 A in association with a competitive peptide inhibitor of the urokinase type plasminogen activator ( uPA ) uPAR interaction . uPAR is composed of three consecutive three finger domains organized in an almost circular manner , which generates both a deep internal cavity where the peptide binds in a helical conformation , and a large external surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We determined that BRMS 1 expression was inversely correlated with that of urokinase type plasminogen activator ( uPA ) , a prometastatic gene that is regulated at least in part by nuclear factor kappaB ( NF kappaB ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A plasmin inhibitor ( alpha N acetyl L lysine methyl ester ) , a specific urokinase type plasminogen activator ( uPA ) inhibitor ( amiloride ) and an anti catalytic anti uPA antibody all caused decreases in the tissue content of active TGF beta and increases in smooth muscle cell death in the media and intima . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : Urokinase plasminogen activator ( uPA ) a key serine protease during remodeling , is capable of inducing both smooth muscle cell migration and proliferation . ^^^ CLINICAL RELEVANCE : Elevated urokinase like plasminogen activator ( uPA ) and decreased plasminogen activator inhibitor 1 ( PAI 1 ) levels are predictors for restenosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor cell migration and invasion into the surrounding extracellular matrix is facilitated by a variety of cell surface associated proteolytic enzymes : matrix metalloproteinases ( MMPs ) , cysteine proteases including cathepsins B and L , aspartic protease cathepsin D , and serine proteases including plasmin and urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumour associated urokinase type plasminogen activator ( uPA ) and its inhibitor PAI 1 in normal and neoplastic tissues of patients with squamous cell cancer of the oral cavity clinical relevance and prognostic value . ^^^ The central role of the serine protease urokinase type plasminogen activator ( uPA ) and its inhibitor , the plasminogen activator inhibitor 1 ( PAI 1 ) , in tumour invasion and metastasis becomes more and more evident . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of the urokinase type plasminogen activator ( uPA ) is under tight regulation by hormones , cytokines and growth factors under physiological conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To evaluate the potential role of the uPAR / uPA / PAI 1 system in HIV induced blood brain barrier ( BBB ) disruption , CSF uPA dependent plasminogen activation ( PdPA ) was analyzed by casein zymography , and CSF protein levels of all three molecules were measured by ELISA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This decreased invasiveness in 5 and 10 mm glucose was paralleled by inhibition of a plasminogen activator ( PA ) activity corresponding to active urokinase type PA ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The matrix metalloproteinase 3 ( MMP 3 ) and urokinase type plasminogen activator ( uPA ) are typical ets 1 responsive genes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Conjunctival PAI 2 was active , as shown by its ability to complex with a target enzyme , urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We created PrAg mutants with mutations affecting different LF binding subsites and containing either urokinase plasminogen activator ( uPA ) or matrix metalloproteinase ( MMP ) cleavage sites . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Alteration in production of matrix metalloproteinase ( MMP ) 2 , MMP 9 and urokinase type plasminogen activator ( uPA ) due to exposure to a laparoscopic environment was determined by zymography and correlated to invasive capacity by a standard Matrigel based invasion assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TGF beta 1 , leptin , collagenase ( MMP 1 ) , and urokinase type plasminogen activator ( uPA ) were assessed in supernatants by ELISA , and MMP 2 and MMP 9 by zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) activity was determined by a coupled colorimetric assay measuring the activity of generated plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
AIM : To investigate the relationship between matrix metalloproteinase 2 ( MMP 2 ) mRNA expression and clinicopathologic and urokinase type plasminogen activator ( uPA ) system parameter and prognosis in human gastric cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( urokinase , uPA ) and its receptor , uPAR , have been implicated in cell adhesion , migration , tissue remodelling and tumour cell invasion . uPAR has three domains and is anchored to membranes by a glycosyl phosphatidylinositol ( GPI ) anchor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : E cadherin ( ECD ) and urokinase plasminogen activator ( uPA ) have been noted as markers for tumor metastasis and prognosis in several tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease that catalyzes the conversion of plasminogen to plasmin . ^^^ The plasminogen / plasmin system includes the uPA , its receptor , and its inhibitor ( plasminogen activator inhibitor 1 ) . ^^^ In previous studies , we found that uPA regulates cell surface fibrinolysis by regulating its own expression as well as that of the uPA receptor and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we show that in MCs , the urokinase type plasminogen activator ( uPA ) induces , via its specific receptor ( uPAR , CD 87 ) , upregulated expression of the complement anaphylatoxin C5a receptor ( C5aR , CD 88 ) , and modulates C5a dependent functional responses . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One proteolytic system involved in these processes is the urokinase type plasminogen activator ( uPA ) system , which consists of uPA , uPA receptor ( uPAR ) and uPA inhibitors 1 and 2 ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator ( PA ) system comprises the 2 serine proteases , urokinase PA ( uPA ) and tissue PA ( tPA ) , the 2 serpin inhibitors , PAI 1 and PAI 2 and the uPA receptor ( uPAR ; CD 87 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to define the role of As2O3 in regulating the tumor cell invasiveness , the effects of As2O3 on secretion of matrix metalloproteinases ( MMPs ) and urokinase plasminogen activator ( uPA ) , and in vitro invasion of HT 1080 human fibrosarcoma cells were examined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study attempted to verify the existence of a relationship between SOX documented by Cu / Zn superoxide dismutase ( Cu / Zn SOD ) and fibrinolysis analyzed by tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasmin / antiplasmin ( PAP ) complexes in dialysis patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activation system in skeletal muscle regeneration : antagonistic roles of urokinase type plasminogen activator ( uPA ) and its inhibitor ( PAI 1 ) . ^^^ Skeletal muscle regeneration induced by injury has been analyzed in urokinase type plasminogen activator ( uPA ) , tissue type plasminogen activator ( tPA ) , plasminogen ( Plg ) and plasminogen activator inhibitor 1 ( PAI 1 ) deficient mice and has demonstrated profound effects of these molecules on the fibrotic state and the inflammatory response , which contribute to muscle repair . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cell associated fibrinolytic system ( urokinase type plasminogen activator , uPA ; uPA receptor , uPAR ; plasminogen activator inhibitor type 1 , PAI 1 ) is pivotal in cell invasion and proliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aims of this study were to investigate the gene expression of fibrinolytic factors urokinase plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in the articular cartilage of rabbit temporomandibular joint ( TMJ ) with disc displacement ( DD ) and to probe the relationship between fibrinolytic activity and cartilage remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we report that prostate carcinoma cells LNCaP and PC 3 autoactivate latent full length PDGF D into its active form under serum independent conditions and that this autoactivation is inhibited by PAI 1 , a urokinase plasminogen activator ( uPA ) / tissue plasminogen activator ( tPA ) inhibitor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a serine protease that not only displays fibrinolytic function but also modulates innate and adaptive immune responses . ^^^ Importantly , the bactericidal properties of uPA are associated with the serine protease domain of the molecule but are not dependent on its plasminogen activation potential and can not be inhibited by plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To investigate the effect of combinational delivery of urokinase type plasminogen activator ( uPA ) and hepatocyte growth factor ( HGF ) genes on hepatic fibrosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : Extracellular fibrinolysis , controlled by the cell associated fibrinolytic system ( urokinase plasminogen activator , uPA ; uPA receptor , uPAR ; plasminogen activator inhibitor type 1 , PAI 1 ) , is involved in cartilage damage generation and in rheumatoid arthritis ( RA ) synovitis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transforming growth factor beta 1 ( TGF beta 1 ) directly activates Src kinase , which in turn activates ERK phosphatidylinositol 3 kinase / Akt , the downstream targets of Src , for urokinase type plasminogen activator ( uPA ) up regulation in human ovarian cancer HRA cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : Urokinase type plasminogen activator ( uPA ) has an important role in tumor progression through the degradation of extracellular matrix . ^^^ In addition , uPA receptor ( uPAR ) and plasminogen activator inhibitors ( PAIs ) , composed of PAI 1 and 2 , are also known to affect such activities . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here we found that the expression of epiregulin , urokinase type plasminogen activator ( uPA ) , matrix metalloproteinase ( MMP ) 14 , and tissue inhibitor of metalloproteinase ( TIMP 2 ) were consistently and progressively up regulated when viewed as a function of tumor stage in tissues of patients versus the metastatic potential seen in the mouse lung model . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) receptor ( uPAR ) has been implicated in signal transduction and biological processes including cancer metastasis , angiogenesis , cell migration , and wound healing . ^^^ It is a specific cell surface receptor for its ligand uPA , which catalyzes the formation of plasmin from plasminogen , thereby activating the proteolytic cascade that contributes to the breakdown of extracellular matrix , a key step in cancer metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) seems to be an important protease in prostate cancer invasion , and tyrosine phosphorylation is thought to play a role in the regulation of its production . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , a widely acting serine protease degrades the ECM components and plays a pivotal role in cancer progression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 ( PAI 1 ) protein levels were not significantly different from sham over time ; however , the ratio of uPA to PAI 1 was decreased through 8 days . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A 6 , a urokinase plasminogen activator ( uPA ) derived peptide in patients with advanced gynecologic cancer : a phase 1 trial . ^^^ OBJECTIVES : The aim of this study was to define the toxicity , maximum feasible dose ( MFD ) , and pharmacokinetics ( PK ) of A 6 , a peptide derived from human urokinase plasminogen activator ( uPA ) , in patients with advanced gynecologic cancers , and to explore anti tumor activity and the effects of A 6 on biomarkers of the urokinase system . ^^^ Serial blood specimens were obtained for pharmacokinetics and levels of urokinase plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) is a serine protease that catalyzes the conversion of plasminogen to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Real time quantitative RT PCR showed that in comparison to SGs , FGs contained elevated expression of epidermal growth factor receptor type 1 ( HER 1 ) , urokinase plasminogen activator ( uPA ) , thymidine phosphorylase ( TP ) and thymidilate synthase ( TS ) mRNAs expression and low levels of 5alpha reductase 2 ( 5alpha R 2 ) mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These animals developed chronic disease as a result of steadily increased inflammation , increased levels of urokinase type plasminogen activator ( uPA ) , and greater degree of demyelination . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we injected kainic acid ( KA ) , a glutamate receptor agonist that specifically hyperstimulates non NMDA type receptors , into the vitreous humor of CD 1 mice and have investigated the role of plasminogen activators ( PAs ) [ tissue plasminogen activator ( tPA ) and urokinase plasminogen activator ( uPA ) ] in excitotoxicity induced retinal damage . ^^^ Injection of KA into the vitreous humor led to an up regulation in tPA and an induction in uPA activity in the retina and this was associated with activation of zymogen plasminogen to active plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
On the 14th day adhesions were evaluated and tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , plasminogen activator inhibitor ( PAI ) type 1 and 2 were measured in peritoneal biopsy specimens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) regulates the proteolytic activity of urokinase type plasminogen activator ( uPA ) and there is increasing evidence for a PAI 1 role in regulating cell migration / invasion by other mechanisms like vitronectin ( VN ) binding and lipoprotein receptor related protein ( LRP ) binding . ^^^ However , manipulation of the endogenous plasminogen activator system ( using blocking antibodies to PAI 1 and to uPA ) in wound induced SKOV 3 cell migration demonstrated an important role for uPA inhibition by PAI 1 that was dependent on plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) system plays a central role in extracellular matrix degradation , cell migration , and invasion . uPA belongs to the family of serine proteases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In eighty seven patients with CRC , the levels of IL 8 , and VEGF as representative angiogenic factors and urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and PAI 2 as representative invasive factors were quantitatively assayed in tumor and adjacent normal tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Green tea polyphenols modulate secretion of urokinase plasminogen activator ( uPA ) and inhibit invasive behavior of breast cancer cells . ^^^ These anti invasive effects of GTP were the result of the inhibition of constitutively active transcription factors AP 1 and NF kappaB , which further suppressed secretion of urokinase plasminogen activator ( uPA ) from breast cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
EEIIMD blocked dilation to the plasminogen activator agonists uPA and tPA while responses to SNP and papaverine were unchanged . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a multifunctional extracellular serine protease implicated in different events including fibrinolysis , tissue remodeling , and hematopoiesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Memantine , which is known to be a moderate affinity , uncompetitive , NMDA receptor antagonist , was investigated with regard to its ability to block the glutamate overstimulation and tissue plasminogen activator ( tPA ) / urokinase plasminogen activator ( uPA ) / matrix metalloproteinase ( MMP ) 9 modulation in experimental ICH . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) plays an important role in the destruction of the extracellular matrix and basement membrane . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , when tyrosine phosphorylation or urokinase type plasminogen activator ( uPA ) / uPAR function was inhibited , both keratinocyte migration and p16INK4a expression were reduced . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we investigated the effect of maspin on the regulation of hypoxia induced expression of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) , with respect to invasive potential in metastatic breast cells MDA MB 231 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) mediated signaling have been implicated in tumor cell invasion , survival , and metastasis in a variety of cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There have been reports of strong correlations between poor prognosis in various cancers and concomitant expression of urokinase type plasminogen activator ( uPA ) and its surface receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interestingly , we observed that fibrin also induced the expression of tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) and plasminogen activator inhibitor 1 by casein zymographic and reverse zymographic analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Pancreatic carcinomas express high levels of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) , both of which mediate cell migration and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To find new principles for inhibiting serine proteases , we screened phage displayed random peptide repertoires with urokinase type plasminogen activator ( uPA ) as the target . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
IC induced inflammation is mediated by inflammatory cell infiltration , a process highly regulated by the cell surface specific receptor ( uPAR ) , a binding partner for the urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : The rat model of portal branch ligation ( PBL ) was compared with partial hepatectomy ( PH ) and sham operation ( SO ) and evaluated for the expression of heat shock protein 70 ( hsp 70 ) , heme oxygenase 1 ( hmox 1 ) , early growth response gene 1 ( Egr 1 ) and urokinase type plasminogen activator ( uPA ) , its inhibitor ( PAI 1 ) and receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The overexpression of proteases , such as urokinase type plasminogen activator ( uPA ) , its receptor ( uPAR ) , and matrix metalloproteinases ( MMP ) , is correlated with the progression of lung cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Celecoxib inhibits urokinase type plasminogen activator ( uPA ) production in MDA MB 231 breast cancer cells . ^^^ Elevated urokinase type plasminogen activator ( uPA ) expression in breast tumors predicts poor survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expressions and clinical significance of urokinase type plasminogen activator ( uPA ) and uPA receptor ( uPAR ) in laryngeal squamous cell carcinoma ( LSCC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human urokinase type plasminogen activator ( uPA ) is poorly secreted and aggregates in the endoplasmic reticulum of yeast cells due to inefficient folding . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( uPA ) is a protein involved in tissue remodeling and other biological processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : After 24 hours ' incubation , tissue plasminogen activator ( tPA ) , urokinase plasminogen activator ( uPA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) levels were determined in medium and cell lysates by using ELISA techniques . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The importance of this residue for inhibition of the PAI 2 protease target urinary plasminogen activator ( urokinase , uPA ) is confirmed , although a high degree of tolerance to P 8 substitution is observed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cells cultured three dimensionally differentiated into an aggressively invasive cell population characterized by the upregulation of matrix metalloproteinase 2 ( MMP 2 ) , MMP 3 , MMP 9 and urokinase type plasminogen activator ( uPA ) secretion and activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Levels of sputum interleukin ( IL ) 8 , IL 10 , interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) alpha , human cationic antimicrobial protein 18 ( CAP 18 ) , urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) , and plasminogen activator inhibitor ( PAI ) 1 were determined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The most upregulated gene identified encodes plasminogen activator inhibitor 1 ( PAI 1 , Serpine 1 ) , a protease inhibitor that blocks urokinase plasminogen activator ( uPA ) and tissue type plasminogen activator ( tPA ) activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We hypothesized that 1 ) culture in 3 % oxygen inhibits EVT invasion compared with culture at 8 % or 20 % oxygen and 2 ) inhibition of invasion is due to alterations in levels of components of the urokinase plasminogen activator ( PLAU , uPA ) system rather than through increased apoptosis and / or decreased proliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) binding to uPAR induces migration , adhesion , and proliferation through multiple interactions with G proteins coupled receptor FPRL 1 , integrins , or the epidermal growth factor ( EGF ) receptor ( EGFR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This association has been intriguing since PAI 1 is known to inhibit urokinase plasminogen activator ( uPA ) that converts plasminogen to plasmin , which is actively involved in tumor progression and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This may activate urokinase plasminogen activator ( uPA ) and gelatinase enzymes , which play a crucial role in trophoblast invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The increased levels of urokinase type plasminogen activator ( uPA ) , uPA receptor ( uPAR ) and type 1 PA inhibitor ( PAI 1 ) are reported in human renal cell carcinoma ( RCC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of the study was to assess the expression of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) in neointima of polyester vascular grafts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to reveal the antithrombotic mechanism of propylene glycol mannite sulfate ( PGMS ) on gene level and explore the correlation between PGMS and fibrinolytic system , the urokinase type plasminogen activator ( uPA ) mRNA expression and uPA activity were detected in vitro and in vivo with semi quantitative reverse transcription ( RT ) PCR and chromogenic method . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : MB 49 murine bladder cancer cells were cultured in media supplemented with resveratrol , rutin , morin , quercetin , gallic acid and tannic acid ( all of them are polyphenols usually present in Mediterranean diet ) for periods of 24 , 48 and 72 hours to quantify the expression of urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) in the culture medium , as well as of metalloproteinase 9 ( MMP 9 ) and cell proliferation . ^^^ The cells in the media supplemented with the nutrients to study did not show inhibition of mRNA expression of urokinase type plasminogen activator ( uPA ) or its high affinity receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The most commonly implicated protease in these processes is urokinase type plasminogen activator ( uPA ) , which is known to be expressed in a number of malignancies including breast and prostate cancer and is directly associated with the higher invasive and metastatic potential of malignancies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To verify the applicability of the cell invasion model , multilayer cell constructs of oral squamous cell carcinoma and oral mucosal fibroblasts were exposed to extrinsic urokinase type plasminogen activator ( uPA ) or plasminogen activator inhibitor ( PAI 1 ) , which are known effectors of cell migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This turnover is regulated by matrix metalloproteinases ( MMPs ) , tissue inhibitor of matrix metalloproteinases ( TIMPs ) , and the plasminogen activation system , including tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
DTAT 13 , a novel recombinant bispecific immunotoxin ( IT ) consisting of truncated diphtheria toxin , an amino terminal ( AT ) fragment of the urokinase type plasminogen activator ( uPa ) , and a fragment of human IL 13 was assembled in order to target receptors on glioblastoma multiforme ( GBM ) and its associated neovasculature . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previously , we showed that bikunin , a Kunitz type protease inhibitor , inhibits invasion and metastasis in several types of cancer cells possibly through suppression of upregulation of urokinase type plasminogen activator ( uPA ) expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Invasion assay was performed to examine the invasiveness of tumor cells induced by EGF , and the expression of matrix metalloproteinase ( MMP ) 9 , MMP 2 , and plasminogen activator ( uPA ) was determined by reverse transcription polymerase chain reaction and Western blot . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Also , in the carcinomas , a significant correlation was found between both forms of Ets 2 protein and urokinase plasminogen activator ( uPA ) ( for p 52 , r = 0 . 43 , P = 0 . 0005 , n = 68 ; for p 54 , r = 0 . 50 , P = 0 . 0001 , n = 68 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since tumor metastasis requires degradation of the extracellular matrix by the serine protease urokinase type plasminogen activator ( uPA ) , we examined the role of LIMK 1 in the regulation of uPA / uPAR system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since the role of urokinase type plasminogen activator ( uPA ) in invasion of PC has been established , we tested the hypothesis that CT increases invasion of PC cells by stimulating uPA secretion from PC cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In our previous study , [ Deamino Cys 1 , D Arg 8 ] vasopressin ( dDAVP ) induced urokinase type plasminogen activator ( uPA ) release from human peripheral T lymphocytes via arginine vasopressin ( AVP ) V 2 receptor mediated reaction enhanced by an AVP V 1 receptor antagonist . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The components of the plasminogen activator ( PA ) system ( urokinase type PA , uPA ; PA inhibitors , PAI 1 / 2 ; uPA receptor , uPAR ) have been implicated in the local degradation of the extracellular matrix ( ECM ) and PCa progression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , normal and malignant liver tissues were evaluated for markers known to be involved in tumor progression and metastasis [ urokinase plasminogen activator ( uPA ) , Her 2 / neu , epidermal growth factor receptor ( EGF R ) ] using sandwich enzyme immunoassays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We wanted to study plasminogen activators , urokinase ( uPA ) and tissue type ( tPA ) and their inhibitor PAI 1 , which have not earlier been studied comprehensively in cutaneous NF 1 related tumors . ^^^ We analyzed the distribution of uPA , tPA and PAI 1 antigen level by immunohistochemistry and mRNA level by in situ hybridization , to identify which cells are primarily involved in proteolytic activity and plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( tPA ) and urokinase type plasminogen activator ( uPA ) may play a role in the pathogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) , a trypsin like serine protease , has been implicated in large number of malignancies , tumor cell invasion , angiogenesis and metastasis ; hence , the potent and selective inhibitors of uPA may therefore be therapeutically useful drugs for treatment of various forms of cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVES : To evaluate the frequency of the urokinase type plasminogen activator ( uPA ) gene amplification and the sensitivity of prostate cancer cells to uPA inhibition , as we previously found one hormone refractory prostate tumour with high level amplification of the uPA ( alias PLAU ) gene , and also showed that a uPA inhibitor , amiloride , can effectively reduce the invasion potential of the PC 3 prostate cancer cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previously , we have generated a potent prodrug consisting of modified anthrax toxins that is activated by urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Nuclear translocation of FXR is concurrent with the increase of urokinase type plasminogen activator ( uPA ) and the formation of F actin , two factors critical for the migration of breast cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A multiplexed fluorescence immunoassay using a novel planar waveguide technology based microarray system , ZeptoMARK ( Zeptosens ) , was developed to detect simultaneously urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and vascular endothelial growth factor ( VEGF ) in extracts of breast cancer tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To study the effect of Parthenolide on the activity and expression of urokinase type plasminogen activator ( uPA ) on PGCL 3 cells and to investigate the antitumor mechanisms of Parthenolide . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : We showed previously that increased urokinase plasminogen activator ( uPA ) expression contributes to vascular smooth muscle cell ( VSMC ) proliferation and neointima formation after injury . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The efficient inactivation of urokinase plasminogen activator ( uPA ) by plasminogen activator inhibitor type 2 ( PAI 2 ) at the surface of carcinoma cells is followed by rapid endocytosis of the uPA PAI 2 complex . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The activities of tissue and urokinase plasminogen activators ( tPA and uPA , respectively ) , the relative inhibition of tPA , and the amounts of plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 , respectively ) in cell free saliva were studied . ^^^ Tissue plasminogen activator activity was found in most samples , with a mean activity of 0 . 63 IU ml ( 1 ) . uPA was observed in only a few samples . ^^^ Tissue plasminogen activator , PAI 1 , and PAI 2 were evident in salivary gland tissue , whereas the expression of uPA was low . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We previously demonstrated the doxorubicin induced expression of urokinase type plasminogen activator ( uPA ) , interleukin 8 ( IL 8 ) , monocyte chemoattractant protein 1 ( MCP 1 ) and tumor necrosis factor alpha in human RC K 8 lymphoma cells and NCI H 69 small cell lung carcinoma cells in which reactive oxygen species might be involved . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study examined the ability of IFNgamma and TNFalpha to stimulate a better invasiveness in B 16 murine melanoma cells , and investigated whether this enhanced ability was related to a higher expression of protease activities , such as urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) , and matrix metalloproteinases 2 and 9 ( MMP 2 , MMP 9 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase type plasminogen activator ( uPA ) plays a major role in extracellular proteolytic events associated with tumor cell growth , migration and angiogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Moreover , Syk and Lck play differential roles in regulating Sp 1 activation and expressions of melanoma cell adhesion molecule ( MelCAM ) , urokinase type plasminogen activator ( uPA ) , matrix metalloproteinase 9 ( MMP 9 ) , and vascular endothelial growth factor ( VEGF ) in response to H / R . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , using metastatic 4T1 and non metastatic 4T07 murine mammary cancer cell lines , we demonstrate that 4T1 cells exhibit significantly increased OPN , integrin linked kinase ( ILK ) , matrix metalloproteinase 2 ( MMP 2 ) and urokinase type plasminogen activator ( uPA ) expression in contrast to 4T07 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : The urokinase plasminogen activator ( uPA ) and its receptor ( uPAR ) are expressed by pancreatic cancer cells and can be targeted by the plasminogen activator inhibitor type 2 ( PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The effects of the bisphosphonate derivative zoledronic acid ( ZA ) on the circulating levels of matrix metalloproteinase 2 ( MMP 2 ) , matrix metallo proteinases 9 ( MMP 9 ) , cathepsin B ( Cath B ) and urokinase type plasminogen activator ( uPA ) in patients with bone metastasis ( BMTS ) and the possible correlation with the symptomatic response induced by this drug in these patients were evaluated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Gel zymography for matrix metalloproteinases ( MMPs ) and urokinase type plasminogen activator ( uPA ) detection revealed that BMP 2 down regulated the activity of MMP 9 , but not of MMP 2 or uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The determination of the protein content of urokinase type plasminogen activator ( uPA ) and its inhibitor , PAI 1 , in breast cancer tissue extracts is used clinically to identify patients at risk to experience disease recurrence ( metastasis ) or early death . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
For cellular invasive processes during a variety of physio and pathophysiological events , including cancer , a fine tuned balance between the formation and loosening of cell adhesive contacts has to occur , implicating the action of pericellular proteases ; among those , the serine protease , urokinase type plasminogen activator ( uPA ) , its inhibitor PAI 1 , and its cellular receptor uPA R ( CD 87 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Among other proteolytic factors , it includes the serine protease urokinase type plasminogen activator ( uPA ) and its three domain ( D1D2D3 ) receptor uPAR ( CD 87 ) , which focuses plasminogen activation to the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytic enzymes , and especially urokinase plasminogen activator ( uPA ) , play an important role in tumour invasion and metastasis . ^^^ Previously we demonstrated that the production of urokinase plasminogen activator ( uPA ) was decreased by several tyrosine kinase inhibitors ( TKI ) in two prostatic carcinoma cell lines . ^^^ Roles of plasminogen and uPA were examined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In a cell free system , using tissue plasminogen activator ( tPA ) and urokinase plasminogen activator ( uPA ) to activate plasminogen , PAI 1 inhibited plasmin generation induced by both activators , whereas IGFBP 5 prevented the effects of PAI 1 on tPA but not uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Squamous cell carcinoma of the oral cavity is characterized by persistent , disorganized expression of integrin alpha3beta1 and enhanced production of urinary type plasminogen activator ( uPA ) and its receptor ( uPAR ) relative to normal oral mucosa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Bone matrix turnover is regulated by matrix metalloproteinases ( MMPs ) , tissue inhibitors of matrix metalloproteinases ( TIMPs ) , and the plasminogen activation system , including tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator ( uPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Up regulations of hepatocytes growth factor ( HGF ) , its receptor ( c Met ) , and urokinase type plasminogen activator ( uPA ) are correlated with the development and metastasis of cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The rabbit myxoma viral serpin , Serp 1 inhibits urokinase and tissue type plasminogen activators ( uPA and tPA ) , plasmin and factor Xa in vitro and exhibits remarkable anti inflammatory activity in various animal models . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Direct interaction of the kringle domain of urokinase type plasminogen activator ( uPA ) and integrin alpha 5 beta 3 induces signal transduction and enhances plasminogen activation . ^^^ It has been questioned whether there are receptors for urokinase type plasminogen activator ( uPA ) that facilitate plasminogen activation other than the high affinity uPA receptor ( uPAR / CD87 ) since studies of uPAR knockout mice did not support a major role of uPAR in plasminogen activation . uPA also promotes cell adhesion , chemotaxis , and proliferation besides plasminogen activation . ^^^ The binding of the uPA kringle to alpha 5 beta 3 and uPA kringle induced alpha 5 beta 3 dependent cell migration were blocked by homologous plasminogen kringles 1 3 or 1 4 ( angiostatin ) , a known integrin antagonist . ^^^ We studied whether the binding of uPA to integrin alpha 5 beta 3 through the kringle domain plays a role in plasminogen activation . ^^^ On CHO cell depleted of uPAR , uPA enhanced plasminogen activation in a kringle and alpha 5 beta 3 dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Calcitonin inhibits invasion of breast cancer cells : involvement of urokinase type plasminogen activator ( uPA ) and uPA receptor . ^^^ It is well established that the urokinase plasminogen activator ( uPA ) system plays an important role in breast cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of components of the plasminogen activator system [ i . e . , urokinase type plasminogen activator ( uPA ) , plasminogen activator inhibitor 1 , and uPA receptor ] was detected in six human pancreatic cancer cell lines . ^^^ Moreover , activation of PPAR gamma by ligands increased plasminogen activator inhibitor 1 and decreased uPA levels in pancreatic cancer cells , and this was accompanied by a reduction in total urokinase activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Neither FRNK nor FAK shRNA ( approximately 80 % reduced FAK levels ) affected 4T1 proliferation in culture , whereas reduced FAK activity or expression blocked 4T1 cell invasion through Matrigel and resulted in 2 3 fold lower urokinase plasminogen activator ( uPA ) expression . ^^^ Conversely , treatment with plasminogen activator inhibitor 1 or neutralizing antibody to uPA blocked Matrigel invasion of 4T1 control cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We hypothesized that : 1 ) macrophage accumulation and cardiac fibrosis in SR uPA+ / o mice are dependent on localization of uPA by the uPA receptor ( uPAR ) ; 2 ) activation of plasminogen by uPA and subsequent activation of transforming growth factor beta 1 ( TGF beta 1 ) and matrix metalloproteinase ( MMP ) 2 and 9 by plasmin are critical pathways through which uPA expressing macrophages accumulate in the heart and cause fibrosis ; and 3 ) uPA induced cardiac fibrosis can be attenuated by treatment with verapamil . ^^^ Our studies revealed that plasminogen is necessary for uPA induced cardiac fibrosis and macrophage accumulation but uPAR is not . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This effect of glucose deprivation on invasion correlated with increased urokinase plasminogen activator ( uPA ) and plasmin activity . ^^^ Glucose deprivation did not increase the levels of gelatinase and plasminogen activator inhibitor 1 secretion , or the expression of cell associated uPA receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MEKK 1 signaling regulates migration through control of cell adhesion and is required for inducible expression of urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Because LRs can modulate the activity of the urokinase type plasminogen activator ( uPA ) receptor and possibly of the platelet derived growth factor ( PDGF ) receptor , LRs may influence the migration of SMCs through functional modulation of these membrane receptors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied levels of major plasminogen activator inhibitor 1 ( PAI 1 ) , tissue type plasminogen activator ( tPA ) , and urokinase type plasminogen activator ( uPA ) in lungs of preterm infants with RDS . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Matrix metalloproteinases ( MMPs ) and urokinase plasminogen activation ( uPA ) system are shown to be involved in tumor invasion as they help in degradation of extracellular matrix ( ECM ) proteins and thus assist in the movement of cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Anti EGFR agents have a direct activity on tumour cells in which they produce growth inhibition , apoptosis , and reduced invasive capacity through the inhibition of molecules associated with tissue invasion such as urokinase type plasminogen activator ( uPA ) and matrix metalloproteinase ( MMP ) 9 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) and its receptor ( uPAR ) play a critical role in cell migration and angiogenesis by facilitating proteolysis of extracellular matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , we defined the relationship between cyclooxygenase ( COX ) 2 and EP receptor expression , proliferation index ( PI ) , microvessel density ( MVD ) , and expression of metalloproteinase 2 ( MMP 2 ) , urokinase type plasminogen activator ( uPA ) , and exon v 6 containing CD 44 isoform ( CD 44 v6 ) by multivariate analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activity based protein profiling revealed multiple serine hydrolases with altered activity between HT hi / diss and HT lo / diss cells , with the largest difference being the activity of urokinase type plasminogen activator ( uPA ) . ^^^ Inhibition of uPA activity with natural ( plasminogen activator inhibitor 1 ) or synthetic ( amiloride ) inhibitors diminished HT hi / diss Matrigel invasion in vitro and intravasation and metastasis in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although maspin does not directly inhibit urokinase type plasminogen activator ( uPA ) activity , we have shown evidence that maspin may block the pericellular proteolysis mediated by cell surface associated uPA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
For our study , we used this transgenic system to test whether prolonged , lung specific , overexpression of the serine protease urokinase plasminogen activator ( uPA ) would result in alveolar wall destruction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To explore the proangiogenic mechanism of BDNF , we investigated the effects of BDNF on extracellular proteolytic enzymes , including matrix metalloproteinases ( MMPs ) and serine proteases , particularly the urokinase type plasminogen activator ( uPA ) plasmin system in human umbilical vein endothelial cells ( HUVECs ) model . ^^^ The HUVECs were treated with various concentrations of BDNF ( 25 400 ng / ml ) for different ( 6 48 hours ) , reverse transcriptase polymerase chain reaction ( RT PCR ) was used to assay MMP 2 , MMP 9 , TIMP 1 , and TIMP 2 mRNA in HUVECs , and the conditioned medium was analyzed for MMP and uPA activity by gelatin zymography and fibrin zymography , respectively . uPA , plasminogen activator inhibitor ( PAI ) 1 , tissue inhibitors of metalloproteinase ( TIMP ) 1 , and TIMP 2 were quantified by western blotting analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect of annexin A 5 on the release of urokinase type plasminogen activator ( uPA ) from cultured RCE cells was determined by zymography , fluorogenic assay of PA activity , and enzyme linked immunosorbent assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Up regulation of the hepatocyte growth factor ( HGF ) , its transmembrane tyrosine kinase receptor ( c Met ) , and urokinase type plasminogen activator ( uPA ) , is associated with the development and metastasis of various types of cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previously , we have shown that increased expression of urokinase plasminogen activator ( uPA ) correlated with malignancy grade in certain sarcomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The high affinity interaction between the serine protease urokinase type plasminogen activator ( uPA ) and its glycolipid anchored receptor ( uPAR ) represents one of the key regulatory steps in cell surface associated plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , a member of the serine protease inhibitor superfamily , modulates fibrinolysis by interacting with proteolytic mediators , including urokinase plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is implicated in various pathophysiological processes , including extracellular matrix turnover , cell migration and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Generation of angiostatin by platelet membranes was not affected by a matrix metalloproteinase ( MMP ) inhibitor , phenanthroline , but was inhibited by serine proteinase inhibitors aprotinin , leupeptin , plasminogen activator inhibitor 1 , and selective inhibitor of urokinase plasminogen activator ( uPA ) , uPA STOP ( TM ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Correlation analysis between casein ratio and activities of plasminogen activation system of goat milk indicated a significant negative relationship for plasmin ( r = 0 . 64 ) , plasminogen ( r = 0 . 69 ) , and urokinase type plasminogen activator ( uPA ; r = 0 . 78 ) during involution but not during lactation . ^^^ As for the cellular components of plasminogen activation system , there was an increase in immunoreactivity on somatic cells toward both monoclonal antibodies of human uPA and human uPA receptor under involution conditions suggesting their upregulation relative to lactation condition . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Several studies have indicated that urokinase plasminogen activator ( uPA ) and transforming growth factor beta ( TGF beta ) are important factors in a host ' s defence against pulmonary pathogens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The authors determined the levels of vascular endothelial growth factor ( VEGF ) and urokinase type plasminogen activator ( uPA ) in the CSF of patients with leptomeningeal metastases ( LM ; n = 53 ) , cancer patients without LM ( n = 18 ) , and subjects without malignancy ( n = 25 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previous reports have shown that genistein and tyrphostin AG 1478 , two tyrosine kinase inhibitors ( TKIs ) , exert multiple cellular effects in prostate carcinoma cells , e . g . a reduction in the production of urokinase plasminogen activator ( uPA ) and its receptor uPAR , and a decrease in the cells ' ability to invade an artificial basement membrane . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Within the Leydig repertoire , a PCR product was found for plasminogen activators urokinase plasminogen activator ( uPA ) and tissue plasminogen activator ( 8 wk old cells ) , matriptase 2 ( mLTC 1 ) , kallikrein 21 , SERPINA 5 , SERPINB 2 ( primary cultures ) , and serine peptidase inhibitor Kunitz type 2 ( SPINT 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( uPA ) is a serine protease that plays a major role in diverse physiological and pathological processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Genes encoding murine tissue plasminogen activator ( tPA ) , urokinase ( uPA ) , and vector controls were stably transfected into 4T1 murine mammary cancer cells , and cell proliferation in vitro was analyzed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation independent of uPA and tPA maintains wound healing in gene deficient mice . ^^^ Simultaneous ablation of the two known activators of plasminogen ( Plg ) , urokinase type ( uPA ) and the tissue type ( tPA ) , results in a substantial delay in skin wound healing . ^^^ These findings demonstrate that an additional plasminogen activator provides sufficient plasmin activity to sustain the healing process albeit at decreased speed in the absence of uPA , tPA and galardin sensitive MMPs and suggest that pKal plays a role in plasmin generation . . ^^^ However , wound closure and epidermal re epithelialization are significantly less impaired in uPA ; tPA double deficient mice than in Plg deficient mice . ^^^ Skin wounds in uPA ; tPA deficient mice treated with the broad spectrum matrix metalloproteinase ( MMP ) inhibitor galardin ( N [ ( 2R ) 2 ( hydroxamido carbonylmethyl ) 4 methylpentanoyl ] L tryptophan methylamide ) eventually heal , whereas skin wounds in galardin treated Plg deficient mice do not heal . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Clots thus formed are lysed by plasmin , which is activated by tissue type and urokinase type plasminogen activators ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The severe combined immunodeficiency disorder ( SCID ) beige / albumin ( Alb ) urokinase plasminogen activator ( uPA ) mouse containing a human mouse chimeric liver is currently the only small animal model capable of supporting hepatitis C virus ( HCV ) infection . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The amino terminal fragment ( ATF , Ser 1 Glu143 ) of urokinase type plasminogen activator ( uPA ) is responsible for some important functions of uPA , such as receptor binding and chemotactic activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We established that a low expression of LRP at the cell surface was associated to elevated extracellular MMP 2 and urokinase plasminogen activator ( uPA ) activities as well as to high invasiveness properties . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
AIM : To investigate the correlations between the expression of urokinase type plasminogen activator ( uPA ) mRNA , uPA receptor ( uPAR ) mRNA and vascular endothelial growth factor ( VEGF ) protein and clinicopathologic features , microvessel density ( MVD ) and survival time . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The functions of the serpin PAI 1 ( plasminogen activator inhibitor 1 ) are based on molecular interactions with its target proteases uPA and tPA ( urokinase type and tissue type plasminogen activator respectively ) , with vitronectin and with endocytosis receptors of the low density lipoprotein family . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , we describe the development and validation of a urokinase type plasminogen activator ( uPA ) sensitive fluorescence imaging probe . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We also show that alterations in the distribution of VE cadherin and beta catenin occur after IGFII and serum or VN stimulation , and propose that the via VN IGFII effects may be facilitated by interaction of the mannose 6 phosphate / IGFII receptor ( M6P / IGFIIR ) with the urokinase type plasminogen activator receptor ( uPAR ) and its ligand ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the diagnostic value of transforming growth factor beta ( 1 ) ( TGFbeta ( 1 ) ) , vascular endothelial growth factor ( VEGF ) , urokinase type plasminogen activator ( uPA ) , and tissue type plasminogen activator ( tPA ) in CSF for leptomeningeal metastasis ( LM ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin , tissue plasminogen activator ( tPA ) , and urokinase plasminogen activator ( uPA ) activity , plasminogen activator inhibitor 1 ( PAI 1 ) , and alpha 2 antiplasmin ( alpha2AP ) antigen were measured in the AAA wall and juxtamural and luminal aspects of intraluminal thrombus in 18 patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Some gene products involved in the metastatic process [ matrixmetalloproteinase 12 ( MMP 12 ) and urokinase plasminogen activator alpha ( uPA ) ] were found as prognostic markers for the recurrence free interval and survival . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Development of a novel albumin binding prodrug that is cleaved by urokinase type plasminogen activator ( uPA ) . ^^^ A water soluble albumin binding prodrug of doxorubicin [ EMC Gly Gly Gly Arg Arg DOXO ( EMC , 6 maleimidocaproic acid ) ] was developed that is cleaved specifically by the tumor associated protease urokinase type plasminogen activator ( uPA ) . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present study tested the hypothesis that increased expression of the proteinases urokinase type plasminogen activator ( uPA ) and matrix metalloproteinases ( MMPs ) is implicated in cardiac inflammation , injury , and subsequent failure during Coxsackievirus B 3 ( CVB 3 ) induced myocarditis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We show that stimulation of monocytic cells with B . burgdorferi induces the transient production and secretion of urokinase plasminogen activator ( uPA ) , shortly followed by its physiological inhibitor , plasminogen activator inhibitor 2 ( PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We assessed the role of urokinase plasminogen activator ( uPA ) and its proteolytic activity in myofibroblast differentiation and the early proliferation following mechanical injury of the rat carotid adventitia . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It describes a cascading event , triggered by S1P , leading to the transactivation of MMP 9 via neuregulin 1 ( NRG 1 ) , vascular endothelial growth factor ( VEGF ) , and the urokinase type plasminogen activator ( uPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Matrix metalloproteinase 2 ( MMP 2 ) , matrix metalloproteinase 9 ( MMP 9 ) , and urokinase type plasminogen activator ( uPA ) are involved in colorectal cancer invasion and metastasis . ^^^ The activity of uPA was assessed by casein plasminogen zymography . ^^^ In rectal tumors , there was an increased activity of uPA compared with the activity in colon tumors ( P = 0 . 0266 ) , however urokinase type plasminogen activator receptor ( uPAR ) and plasminogen activator inhibitor 1 ( PAI 1 ) showed no significant difference between colon and rectal cancer tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Among them , urokinase type plasminogen activator ( uPA ) exerts a variety of functions during development , and is involved in learning and memory . ^^^ Over expression of uPA in the VTA induces doxycycline dependent expression of its receptor , uPAR , but not its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) . uPAR expression in the VTA is repressed upon silencing of uPA with lentiviruses expressing siRNAs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : Urokinase type plasminogen activator ( uPA ) regulates cell migration and invasion by pericellular proteolysis and signal transduction events . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : One of the most thoroughly studied systems in relation to its prognostic relevance in patients with breast cancer , is the plasminogen activation system that comprises of , among others , the urokinase Plasminogen Activator ( uPA ) and its main inhibitor , the Plasminogen Activator Inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Chromatin immunoprecipitation ( ChIP ) demonstrates that myosin 6 is recruited to the promoter and intragenic regions of active genes , encoding urokinase plasminogen activator ( uPA ) , eukaryotic initiation factor 6 ( p27 / eIF6 ) , and low density lipoprotein receptor ( LDLR ) , but not to noncoding , nonregulatory intergenic regions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : Of the 60 genes measured by qrt PCR , urokinase type plasminogen activator ( uPA or PLAU ) mRNA expression was the most significant marker associated with distant metastasis free survival ( MFS ) by univariate Cox analysis in patients with ErbB 2 positive tumors and an independent factor in multivariate analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This paper describes the structure activity relationship in a series of tripeptidyl diphenyl phosphonate irreversible urokinase plasminogen activator ( uPA ) inhibitors , originally derived from an arginyltripeptide . uPA is considered an interesting target in anticancer drug design . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study we analyzed the role of the c Jun N terminal kinases ( JNK ) pathway in the TGF beta 1 stimulation of urokinase type plasminogen activator ( uPA ) , initial stages of epithelial mesenchymal transdifferentiation ( EMT ) and cell migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To evaluate the role and interaction of plasminogen activators and matrix metalloproteinases ( MMPs ) in arterial remodeling in vivo we compared effects of recombinant urokinase ( uPA ) and tissue type ( tPA ) plasminogen activators on vessel morphology , cell proliferation , inflammatory reaction and MMPs expression in arterial wall after experimental balloon angioplasty . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator mRNA is strongly expressed by many classes of neurons in peripheral and central nervous system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The rationale of prevention of thrombosis by enhancing blood levels of single chain urokinase type plasminogen activator ( scuPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Potential approaches to improve thrombolytic agents comprise the construction of mutants and variants of tissue type plasminogen activator ( tPA ) or of single chain urokinase type plasminogen activator ( scuPA , pro urokinase ) , of chimeric plasminogen activators and of conjugates of plasminogen activators with monoclonal antibodies . tPA mutants have been constructed with altered pharmacokinetic properties or altered functional properties , including binding to and stimulation by fibrin , resistance to plasmin and to protease inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study evaluates the cell surface expression of urokinase type plasminogen activator ( u PA ) and the capacity to bind exogenous urokinase as possible parameters for the distinction of various types of human lung tumours . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Heparin inhibits smooth muscle cell proliferation and suppresses the induction of tPA mRNA and protein while it has little effect on the mRNA of urokinase type plasminogen activator , plasminogen activator inhibitor type 1 , and a number of genes that are also modulated by serum and phorbol esters . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Assignment of the urokinase type plasminogen activator receptor gene ( PLAUR ) to chromosome 19q13 . 1 q13 . 2 . ^^^ The urokinase type plasminogen activator receptor ( uPAR ) is a key molecule in the regulation of cell surface plasminogen activation and , as such , plays an important role in many normal as well as pathological processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Phosphorylation of a surface receptor bound urokinase type plasminogen activator in a human metastatic carcinomatous cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The specific phosphatase inhibitor , okadaic acid , increases the level of mRNA for the receptor for urokinase type plasminogen activator ( u PAR ) in 8 out of 13 human cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase type plasminogen activator is deficient on peripheral blood leukocytes in patients with paroxysmal nocturnal hemoglobinuria . ^^^ We report here that the cellular receptor for urokinase type plasminogen activator ( u PAR ) is deficient on affected peripheral blood monocytes and granulocytes from four individuals with PNH as evidenced by chemical cross linking analysis as well as by immunofluorescence flow cytometry using a monoclonal anti u PAR antibody . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A recombinant chimeric plasminogen activator , MA 15C5Hu / scu PA 32k , composed of a humanized fibrin fragment D dimer specific monoclonal antibody ( MA 15C5Hu ) and a recombinant low molecular mass single chain urokinase type plasminogen activator , comprising amino acids Leu 144 Leu411 ( scu PA 32k ) , was produced by cotransfecting Chinese hamster ovary ( CHO ) cells with the cDNA encoding the MA 15C5Hu light chain sequence and the cDNA encoding the MA 15C5Hu heavy chain sequence fused with the cDNA encoding scu PA 32k . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The catabolism of t PA was not inhibited by an excess of urokinase type plasminogen activator ( u PA ) , and t PA bound to Novikoff membranes was not complexed to PAI 1 , suggesting a mechanism independent of PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This enzyme was apparently different from urokinase type plasminogen activator or cathepsin B and was present in mammary tumour at levels at least 20 times higher than those in normal mammary tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activities and mRNA levels of tissue type and urokinase type plasminogen activator were increased in a reproducible pattern during differentiation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombin cleaves single chain urokinase type plasminogen activator ( scu PA ) to a two chain derivative ( tcu PA ) fibrinolytically inactive . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect of the binding of the single chain chimeric plasminogen activator t PA / scu PA , which contains amino acids 1 to 274 of tissue type plasminogen activator ( t PA ) and amino acids 138 to 411 of single chain urokinase type plasminogen activator ( scu PA ) , to fibrin on its biochemical properties was investigated in a purified system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator and its specific receptor in high metastatic and non metastatic cell lines derived from human lung adenocarcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Invasion of brain tissue by primary glioma : evidence for the involvement of urokinase type plasminogen activator as an activator of type 4 collagenase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using an immortalized human glomerular epithelial cell line ( E 71 A1 ) , we studied the effect of endothelin 1 ( ET 1 ) , a potent vasoconstrictor peptide , on the synthesis of urokinase type plasminogen activator ( u PA ) and its receptor ( u PAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of the urokinase type plasminogen activator receptor on vascular smooth muscle cells is under the control of thrombin and other mitogens . ^^^ The urokinase type plasminogen activator receptor ( u PAR ) was demonstrated on cultured smooth muscle cells ( SMCs ) of bovine aorta . ^^^ Binding of 125I urokinase type plasminogen activator ( u PA ) was concentration dependent and saturable within 45 60 minutes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Hepatocyte growth factor increases urokinase type plasminogen activator ( u PA ) and u PA receptor expression in Madin Darby canine kidney epithelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The parameters studied are cellular procoagulant activity , secretion of plasminogen activator inhibitor ( PAI 1 ) and urokinase type plasminogen activator ( u PA ) , activation and internalization of factor 10 and Merocyanine 540 staining . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have shown the presence of surface receptors for the amino terminal fragment ( ATF ) of human urokinase type plasminogen activator ( u PA ) on an in vitro established cell line of human epidermal origin by both radio binding assays with human 125I u PA ATF and transmission electron microscopy of a gold u PA complex . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two plasminogen activators ( PAs ) : tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) , as well as the type 1 plasminogen activator inhibitor ( PAI 1 ) are synthesized and secreted by rat astrocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TNF alpha and LPS inhibited both CSF 1 stimulated urokinase type plasminogen activator ( u PA ) mRNA levels and u PA activity in BMM , whereas IFN gamma lowered only the u PA activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparative thrombolytic properties of tissue type plasminogen activator ( t PA ) , single chain urokinase type plasminogen activator ( u PA ) and K1K2Pu ( a t PA / u PA chimera ) in a combined arterial and venous thrombosis model in the dog . ^^^ The chimeric molecule K1K2Pu , comprising the two kringle domains ( K 1 and K 2 ) of tissue type plasminogen activator ( t PA ) and the COOH terminal region with the serine protease domain ( Pu ) of urokinase type plasminogen activator ( u PA ) , was previously shown to have a 5 to 10 fold reduced clearance rate with maintained specific thrombolytic activity , resulting in an increased thrombolytic potency in animal models of venous and arterial thrombosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two PstI polymorphisms for the urokinase type plasminogen activator receptor gene ( PLAUR ) . ^^^ The cDNA probe puPAR 2 detects two PstI polymorphisms in the urokinase type plasminogen activator receptor gene ( PLAUR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Because these molecules lack specificity for the fibrin clot , important efforts have been made to produce , using recombinant DNA technology , agents presenting higher fibrin clot selectivity such as t PA ( tissue type plasminogen activator ) and scu PA ( single chain urokinase type plasminogen activator ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have used a 20 liter stirred tank fermentor , equipped with a 127 mesh ethylene tetrafluoroethylene rotating screen for cell recycle , for the continuous production of recombinant single chain urokinase type plasminogen activator ( rscu PA ) from Chinese hamster ovary ( CHO ) cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An expression cassette containing a synonymous gene for human single chain urokinase type plasminogen activator ( Rscu PA ) 5 ' flanked by a trp promoter and the Shine Dalgarno sequence of the xyl A operon of Bacillus subtilis and terminated by the terminators trp A and Tn 10 was constructed and inserted into a pBR 322 derivative to yield pBF 160 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Determination of plasma urokinase type plasminogen activator antigen in patients with primary liver cancer : characterization as tumor associated antigen and comparison with alpha fetoprotein . ^^^ We determined the plasma levels of urokinase type plasminogen activator ( u PA ) antigen and alpha fetoprotein ( AFP ) in 44 patients with different stages of liver cirrhosis and in 29 patients with liver cirrhosis based primary liver cancer at the time of first clinical detection of the malignant disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Identification and characterization of the murine cell surface receptor for the urokinase type plasminogen activator . ^^^ Cell binding experiments have indicated that murine cells on their surface have specific binding sites for mouse urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These cells expressed cytokeratin , placental alkaline phosphatase , urokinase type plasminogen activator , and pregnancy specific beta glycoprotein , but not factor 8 or 63D3 ; they also produced hCG and collagenase type 4 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Endometrial tissue explants in culture were found to release urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An enzyme linked immunosorbent assay for urokinase type plasminogen activator ( u PA ) and mutants and chimeras containing the serine protease domain of u PA . ^^^ An enzyme linked immunosorbent assay ( ELISA ) for quantitation of natural and recombinant plasminogen activators containing the serine protease domain ( B chain ) of urokinase type plasminogen activator ( u PA ) was developed , based on two murine monoclonal antibodies , MA 4D1E8 and MA 2L3 , raised against u PA and reacting with non overlapping epitopes in the B chain . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Vitronectin regulates the synthesis and localization of urokinase type plasminogen activator in HT 1080 cells . ^^^ The effect of extracellular matrix composition on the location , amount , and activity of cell associated urokinase type plasminogen activator was tested using HT 1080 cells adherent to either fibronectin or vitronectin . ^^^ Examination of both HT 1080 and normal human fibroblast cells by immunofluorescent microscopy localized urokinase type plasminogen activator to discrete , focal areas underneath cells adherent to vitronectin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When compared to wild type bFGF , M6B bFGF showed in cultured endothelial cells a similar receptor binding capacity and mitogenic activity , but a reduced affinity for heparin like low affinity binding sites , a reduced chemotactic activity , and a reduced capacity to induce the production of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These complexes were probably formed by activation of urokinase type plasminogen activator ( u PA ) , and not tissue type plasminogen activator ( t PA ) , since SF levels of both u PA antigen and u PA plasminogen activator inhibitor ( PAI ) complexes were increased in 27 of the 42 patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cellular receptor for urokinase type plasminogen activator ( uPAR ) is a glycolipid anchored three domain membrane protein playing a central role in pericellular plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have now carried out in vitro experiments with bovine vascular endothelial cells to determine which events occurring during angiogenesis are affected by this microbial angiogenesis inhibitor . 15 Deoxyspergualin did not inhibit the production of urokinase type plasminogen activator ( u PA ) or type 4 collagenase by vascular endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Gamma interferon counteracts interleukin 1 alpha stimulated expression of urokinase type plasminogen activator in human endothelial cells in vitro . ^^^ The effect of gamma interferon ( gamma IFN ) on the interleukin 1 alpha ( IL 1 alpha ) induced stimulation of urokinase type plasminogen activator ( u PA ) expression in human foreskin microvascular endothelial cells ( HFMEC ) and in human umbilical vein endothelial cells ( HUVEC ) was investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biochemical properties of recombinant mutants of nonglycosylated single chain urokinase type plasminogen activator . ^^^ The role of glycosylation on the enzymatic properties of single chain urokinase type plasminogen activator ( scu PA ) was investigated by site specific mutagenesis of the glycosylated Asn 302 residu to Gln . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These results are the first demonstration of the existence of a tumor cell membrane associated putative receptor for a member of the matrix metalloproteinase family , as previously evidenced for the urokinase type plasminogen activator . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A recombinant chimeric plasminogen activator consisting of a humanized monoclonal antibody specific for cross linked human fibrin ( MA 15C5Hu ) and a 32 kDa single chain urokinase type plasminogen activator ( scu PA 32k ) comprising amino acids Leu 144 Leu411 , MA 15C5Hu / scu PA 32k , was previously found to have a 12 fold higher fibrinolytic potency than recombinant scu PA 32k towards a human plasma clot in a human plasma milieu in vitro ( Vandamme et al . , Eur J Biochem 1992 ; 205 : 139 46 ) . ^^^ Therefore , the thrombolytic and pharmacokinetic properties of MA 15C5Hu / scu PA 32k were compared with those of recombinant single chain urokinase type plasminogen activator ( scu PA ) in 3 different venous thrombosis models in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Depression of tissue type plasminogen activator and enhancement of urokinase type plasminogen activator as an expression of local inflammation . ^^^ In contrast , u PA related plasminogen activation was strongly enhanced : urokinase type plasminogen activator ( u PA ) antigen ( 23 . 1 + / 12 . 4 ng / ml ) was more than four fold higher , single chain u PA ( scu PA ) ( 5 . 3 + / 1 . 9 ng / ml ) three fold higher than in plasma of the same patients or of healthy donors , and active two chain u PA ( tcu PA ) was detected in 14 of the 36 SF samples of RA patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
High plasma urokinase type plasminogen activator levels are present in patients with acute nonlymphoblastic leukemia . ^^^ The aim of this work was to evaluate urokinase type plasminogen activator ( u PA ) and related parameters of the fibrinolytic system in 14 ANLL patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator antigen ( t PA : Ag ) , urokinase type plasminogen activator antigen ( u PA : Ag ) , the proenzyme single chain u PA ( scu PA ) , and plasminogen activator inhibitor ( PAI ) were measured in the synovial fluid and plasma of 22 patients with seropositive rheumatoid arthritis ( RA ) , 13 with seronegative RA , and 23 patients with various forms of arthritis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
So far , the use of the more recently developed thrombolytic agents single chain urokinase type plasminogen activator ( scu PA ) and tissue type plasminogen activator ( t PA ) were disappointing , mainly due to some of their negative properties in vivo , i . e . , rapid inhibition and / or hepatic clearance . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Quantitative assay of the carbohydrate in urokinase type plasminogen activator by lectin enzyme immunoassay . ^^^ We developed an enzyme immunoassay ( EIA ) for the measurement of Asn 302 linked carbohydrate in urokinase type plasminogen activator ( u PA ) using peroxidase ( HRP ) labelled lectins . u PA antigen in the sample was immunologically bound to microtitre plate wells by anti u PA IgG and the binding of HRP labelled lectins [ Con A ( Concanavalin A ) , WGA ( wheat germ agglutinin ) , PNA ( peanut agglutinin ) , CSA ( Scotch broom ) , GS 1 ( Groffonia simplicifollia ) and SBA ( soybean agglutinin ) ] to the carbohydrate of u PA was measured . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The pattern of expression of urokinase type plasminogen activator ( PA ) in granulocyte macrophage CSF transgenic mice and their normal littermates was studied using RNAse protection assays and a plasminogen dependent fibrinolytic assay for PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Wound fluid stimulated accumulation of urokinase type plasminogen activator by fibroblasts was also observed in a time dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Retinal pigment epithelial cells secrete urokinase type plasminogen activator and its inhibitor PAI 1 . ^^^ The results showed that these cells produce urokinase type plasminogen activator ( u PA ) and a plasminogen activator inhibitor ( PAI ) which is immunologically and biochemically similar to PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the two HPAs the kringle 2 domain ( K2tu PA ) or the finger and the kringle 2 domains ( FK2tu PA ) of tissue type plasminogen activator ( t PA ) are linked to the catalytic protease domain of single chain urokinase type plasminogen activator ( scu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator activity increases during the growth of two murine mammary adenocarcinomas with different metastasizing abilities . ^^^ Urokinase type plasminogen activator ( u PA ) plays an important role in tumor growth and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The assay is based on the quantitative conversion of PAI 1 to urokinase type plasminogen activator ( u PA ) PAI 1 complex the concentration of which is then determined by an ELISA employing monoclonal anti PAI 1 as catching antibody and monoclonal anti u PA as detecting antibody . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
K12G0S32 is a 57 kDa recombinant single chain chimeric plasminogen activator consisting of scFv K12Go , a single chain variable region antigen binding fragment ( Fv ) of the monoclonal antibody MA 15C5 , which is specific for fragment D dimer of human cross linked fibrin , and a low molecular mass ( 33 kDa ) urokinase type plasminogen activator ( u PA 33k ) containing amino acids Ala 132 Leu411 ( Holvoet , P . , Laroche , Y . , Lijnen , H . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen , plasminogen activators ( PA ) , tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , alpha 2 antiplasmin ( alpha 2 AP ) , plasminogen activator inhibitor ( PAI ) , fibrinogen / fibrin degradation products ( FDP ) and D dimer were determined to elucidate the role of plasminogen activators and inhibitors in the pathogenesis of accelerated fibrinolysis in schistosomiasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Infusion of 15 ng / h of NGF with a mini osmotic pump for 14 days resulted in a 2 fold increase of ABP mRNA as revealed by Northern blots , whereas the mRNA level of another Sertoli cell protein , urokinase type plasminogen activator , remained unchanged . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have examined the prognostic value of the levels in the blood of granulocyte elastase alpha 1 proteinase inhibitor ( E alpha 1 PI ) complex , tumor necrosis factor alpha ( TNF alpha ) and urokinase type plasminogen activator ( u PA ) in 35 patients with severe infection upon admission to an Intensive Care Unit . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Presence of urokinase type plasminogen activator ( u PA ) in tissue extracts of antrochoanal polyp . ^^^ These findings confirmed that the tissue extracts of PMM contained t PA , and that those of AP contained both t PA and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown that urokinase type plasminogen activator ( u PA ) activity is induced in microvascular endothelial cells migrating from the edges of a wounded monolayer in vitro ( Pepper et al . , J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator release after DDAVP in von Willebrand disease : different behaviour of plasminogen activators according to the synthesis of von Willebrand factor . ^^^ We investigated the behaviour of factor VIII / von Willebrand factor measurements , and of tissue plasminogen activator and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At the same time , urokinase type plasminogen activator is stimulated in tissue constituent cells and plasmin activity increases locally . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biochemical properties of recombinant single chain urokinase type plasminogen activator mutants with deletion of Asn 2 through Phe 157 and / or substitution of Cys 279 with Ala . ^^^ The contribution of the NH 2 terminal polypeptide chain and of the Cys 148 Cys279 interchain disulphide bond to the enzyme activity of urokinase type plasminogen activator ( u PA ) was studied using site specific mutagenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Co secretion of plasminogen activator inhibitor type 1 ( PAI 1 ) and urokinase type plasminogen activator was identified in short term cultures of primary type 2 pneumocytes isolated from adult rats . ^^^ Type 2 pneumocyte PAI 1 formed SDS resistant complexes with tissue type and urokinase type plasminogen activator and was found to be stable to acid , to short term exposure to heat , and to the denaturants guanidine HCl and SDS , while being sensitive to treatment with alkali and urea . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Until recently , research aimed at inhibiting metastasis has concentrated on the proteases , especially on urokinase type plasminogen activator and collagenase 4 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Peripheral blood monocytes are essential participants in processes that require pericellular plasminogen activation , a regulated proteolytic pathway that is greatly influenced by the relative concentrations of urokinase type plasminogen activator ( profibrinolytic ) and plasminogen activator inhibitor type 2 ( PAI 2 ) ( anti fibrinolytic ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 2 ( PAI 2 ) is a potent and primary inhibitor of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The content of urokinase type plasminogen activator antigen as a prognostic factor in urinary bladder cancer . ^^^ Urokinase type plasminogen activator ( u PA ) is thought to be implicated in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to the low metastatic Eb line , the metastatic variants ESb and ESb MP secreted urokinase type plasminogen activator ( u PA ) , which was present in the culture supernatant predominantly in the active form ( ESb , 96 % ; ESb MP , 80 % ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As expected , steroidogenesis in these kinase deficient cells was no longer stimulated by corticotropin or cAMP analogues , and the expression of three cAMP regulated genes ( ornithine decarboxylase , urokinase type plasminogen activator , and P 450 side chain cleavage ) could no longer be induced . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombolytic and pharmacokinetic properties of an immunoconjugate of single chain urokinase type plasminogen activator ( u PA ) and a bispecific monoclonal antibody against fibrin and against u PA in baboons . ^^^ The thrombolytic and pharmacokinetic properties of rscu PA / MA FU 1 74 , an immunoconjugate of recombinant single chain urokinase type plasminogen activator ( rscu PA ) and a bispecific MA directed against u PA and against the beta chain of human fibrin ( MA FU 1 74 ) , were investigated in baboons with a [ 125I ] fibrin labeled autologous blood clot in the femoral vein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Pulmonary epithelial cell urokinase type plasminogen activator . ^^^ Because the tissue repair process occurs in an acute inflammatory setting , we investigated the effects of inflammatory mediators on urokinase type plasminogen activator ( u PA ) expression by pulmonary epithelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Other serine proteinases , e . g . urokinase type plasminogen activator and thrombin , also cleaved this `` substrate ' ' form of PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of the present study was to compare plasma levels of urokinase type plasminogen activator ( u PA ) , before and after 20 min of venous stasis , with those of tissue type plasminogen activator ( t PA ) , type 1 plasminogen activator inhibitor ( PAI 1 ) and t PA / PAI 1 complexes , to determine whether both plasminogen activators and their inhibitor respond similarly to the same stimulus . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
K1K2Pu , a recombinant t PA / u PA chimera with increased thrombolytic potency , consisting of amino acids 1 to 3 and 87 to 274 of human tissue type plasminogen activator ( t PA ) and amino acids 138 to 411 of human single chain urokinase type plasminogen activator ( scu PA ) . ^^^ K1K2Pu , a recombinant t PA / u PA chimera with increased thrombolytic potency in animal models of venous and arterial thrombosis , which consists of amino acids 1 to 3 and 87 to 274 of human tissue type plasminogen activator ( t PA ) and amino acids 138 to 411 of human single chain urokinase type plasminogen activator ( scu PA ) , was produced and conditioned for use in patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A similar effect was obtained by addition of urokinase type plasminogen activator ( u PA ) but not with fibrinolysis unrelated enzymes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation by single chain urokinase type plasminogen activator or pro urokinase ( pro UK ) is accompanied by the generation of two chain urokinase ( UK ) by plasmin which provides a positive feedback . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine the relationship between cardiovascular complications of estrogen therapy and fibrinolysis , fibrinolysis parameters plasminogen , urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) , were assessed in 12 prostatic cancer patients before and 6 weeks after the onset of estrogen therapy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to LLC PK 1 cells ( wild type ) , VPR 1 cells exhibited no response to vasopressin in terms of in vitro adenylate cyclase activation , in vivo cAMP production , or urokinase type plasminogen activator induction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PC 12 cells were found to contain and release a 70 75 kDa tissue type plasminogen activator ( tPA ) and a much less abundant 48 kDa urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously reported that normal pleural leukocytes secrete a urokinase type plasminogen activator inhibitor ( PAI ) in culture . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The conditioned media from postsurgical Day 1 macrophage culture strongly inhibited urokinase type plasminogen activator ( PA ) and this plasminogen activator inhibitor ( PAI ) activities decreased following the extension of postsurgical time . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transformation dependent activation of urokinase type plasminogen activator by a plasmin independent mechanism : involvement of cell surface membranes . ^^^ Transformed cells produce elevated levels of the urokinase type plasminogen activator ( u PA ) , which has been linked with the invasive or migratory phenotype of these cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have compared the cell specific expression and regulation of the receptor for urokinase type plasminogen activator ( u PAR ) by transforming growth factor beta type 1 ( TGF beta 1 ) in 10 human cell lines derived from both normal and neoplastic tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There was no apparent effect of EGF on the expression of urokinase type plasminogen activator and 72 kDa type 4 collagenase in IMC 2 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Acceleration of the thrombin inactivation of single chain urokinase type plasminogen activator ( pro urokinase ) by thrombomodulin . ^^^ The in vitro effects of thrombomodulin on the inactivation of single chain urokinase type plasminogen activator ( scu PA ) by thrombin were investigated by incubating scu PA with varying concentrations of human thrombin , in both the absence and presence of soluble rabbit thrombomodulin . 50 % inactivation of scu PA occurred in 45 min at 160 ng / ml thrombin in the absence of thrombomodulin and at 4 . 6 ng / ml thrombin in the presence of thrombomodulin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RA synovium ( compared with that of patients with OA or joint trauma ) had increased numbers of macrophages and increased expression / content of fibrinogen , tissue factor , factor 13 , tissue transglutaminase , cross linked fibrin ( fibrin D dimer ) , urokinase type plasminogen activator , and alpha 2 plasmin inhibitor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They induce fibroblasts and macrophages to produce neutral metalloproteinases such as procollagenase and prostromelysin , the serine proteinase urokinase type plasminogen activator ( u PA ) , tissue inhibitor of metalloproteinase ( TIMP ) , and prostaglandins , u PA converts plasminogen into plasmin , which can activate neutral metalloproteinase proenzymes , and these enzymes degrade the extracellular matrix components . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma samples from 17 patients with endometrial cancer and from 52 patients with cervical carcinoma were determined with respect to their levels of components of the fibrinolytic system ( tissue type plasminogen activator antigen , urokinase type plasminogen activator antigen , plasminogen activator inhibitor activity ) and related to the observed alterations of three acute phase reactants ( C reactive protein , coeruloplasmin , alpha 1 antitrypsin ) . ^^^ As shown previously , uterine malignancies , especially at later stages , exhibited significant increases in plasma levels of urokinase type plasminogen activator antigen as compared to an age matched control group . ^^^ However , the increase in urokinase type plasminogen activator antigen did not correlate with the increase of either C reactive protein or coeruloplasmin plasma level . ^^^ These data indicate that the increase in plasma urokinase type plasminogen activator antigen in patients with uterine malignancies does not follow the pattern of common acute phase reactants , like C reactive protein or coeruloplasmin . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A monoclonal antibody specific for two chain urokinase type plasminogen activator . ^^^ Application to the study of the mechanism of clot lysis with single chain urokinase type plasminogen activator in plasma . ^^^ A murine monoclonal antibody ( MA 12E6A8 ) was raised against human urokinase type plasminogen activator ( u PA ) , which , in an enzyme linked immunosorbent assay ( ELISA ) , reacted 15 , 000 fold better with recombinant two chain u PA ( rtcu PA ) than with recombinant single chain u PA ( rscu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Invasion of tissue by monocytes in the course of cellular immune reactions is a multistep process that is thought to be based on the action of urokinase type plasminogen activator ( u PA ) , an ubiquitous serine protease able to convert the zymogen plasminogen into the active protease plasmin . ^^^ Expression and occupation of urokinase type plasminogen activator receptors ( u PA R ) are known to be up regulated by IFN gamma and TNF alpha , and endogenously occupied u PA R were found to be instrumental in monocyte invasiveness . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When p aminobenzamidine , a serine protease inhibitor which inhibits urokinase type plasminogen activator , was administered together with hCG in the incubation medium , both the permeability increase and PMN extravasation were prevented . ^^^ These results support the concept that the hCG induced increase in vasopermeability in the rat testis is related to extravasation of PMNs and suggest that urokinase type plasminogen activator is involved in migration of these cells through the postcapillary venular walls . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Amiloride is an inhibitor of urokinase type plasminogen activator , and might therefore have an inhibitory effect on neovascularization . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both neoplastic cells and benign hyperplastic glandular epithelial cells stained weakly and in a patchy distribution for tissue factor and focally for low molecular weight urokinase type plasminogen activator . ^^^ By contrast , no tissue staining was observed for factor 7 , factor 10 , factor 13 `` a ' ' subunit , high molecular weight urokinase type plasminogen activator , plasminogen activator inhibitors 1 to 3 , protein C , and protein S . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In anesthetized rabbits with pulmonary embolized thrombi the interactions of saruplase ( recombinant unglycosylated single chain urokinase type plasminogen activator , CAS 99149 95 8 ) with acetylsalicylic acid ( ASA ) , glyceryl trinitrate ( nitroglycerin , GTN ) , heparin , and the antifibrinolytics tranexamic acid and aprotinin have been studied . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purified protein shows a molecular mass of 45 kDa in SDS PAGE , cross reacts with monoclonal antibodies against PAI 2 ( Mab ' PAI 2 ) , and inhibits the amidolytic activity of urokinase type plasminogen activator ( u PA ) towards the chromogenic substrate Glu Gly Arg pNA ( S 2444 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TNF induced a brief fourfold increase in the overall plasma plasminogen activator ( PA ) activity peaking after 1 h ( p less than 0 . 0001 ) , which was associated with rises in the antigenic levels of urokinase type plasminogen activator ( p less than 0 . 0001 ) and tissue type plasminogen activator ( p less than 0 . 0001 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have raised four monoclonal antibodies recognizing different epitopes within the human cell surface receptor for urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They also released urokinase type plasminogen activator , but only half as much as do human umbilical vein endothelial cells ; release of tissue type plasminogen activator was not observed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , it did not significantly modulate urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI 1 ) , and tissue factor ( TF ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although there was no change in urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) increased significantly after 5 min . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Vitronectin endows plasminogen activator inhibitor 1 ( PAI 1 ) , the fast acting inhibitor of both tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) , with additional thrombin inhibitory properties . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vivo and in vitro interaction of high and low molecular weight single chain urokinase type plasminogen activator with rat liver cells . ^^^ The plasma clearance and the interaction of high ( HMW ) and low ( LMW ) molecular weight single chain urokinase type plasminogen activator ( scu PA ) with rat liver cells was determined . 125I Labeled HMW and LMW scu PA were rapidly cleared from plasma with a half life of 0 . 45 min and a maximal liver uptake of 55 % of the injected dose . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We measured antigen levels of 2 kinds of plasminogen activator , tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( UK ) , as well as those of their primary inhibitors , type 1 plasminogen activator inhibitor ( PAI 1 ) and type 2 plasminogen activator inhibitor ( PAI 2 ) , in tissue extracts from benign and malignant breast tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Clot selective coronary thrombolysis with low dose synergistic combinations of single chain urokinase type plasminogen activator and recombinant tissue type plasminogen activator . ^^^ The effect of simultaneous infusions of low dose recombinant tissue type plasminogen activator ( t PA ) and single chain urokinase type plasminogen activator ( scu PA , pro urokinase ) on coronary arterial thrombolysis was investigated in 23 patients treated within 6 hours ( mean 2 . 6 + / 1 . 1 , range 1 . 2 to 5 . 9 ) of symptoms of an acute myocardial infarction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Correlation between urokinase type plasminogen activator production and the metastatic ability of human rectal cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two colonies , named CLF 14 and CLF 8 , exhibited significantly high expression levels of the biological activity of urokinase type plasminogen activator ( mu Pa ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The PAA was due to both types of plasminogen activator ; tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Hybrid molecules containing the catalytic domain of either tissue plasminogen activator ( tPA ) or single chain urokinase type plasminogen activator ( scuPA ) , and the fibrin binding domain of a murine antifibrin monoclonal antibody were constructed using either cDNA or genomic DNA encoding the plasminogen activator and genomic DNA encoding antifibrin monoclonal antibody 59D8 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mutations affecting the activity of urokinase type plasminogen activator . ^^^ Mutagenesis throughout the single chain urokinase type plasminogen activator ( scu PA ) cDNA molecule , followed by expression of the mutant genes and secretion of the resulting mutant proteins from yeast , has been used to determine the amino acid residues important for activity of scu PA molecules . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Forty one patients with acute myocardial infarction received a combination low dose thrombolytic therapy with recombinant tissue type plasminogen activator ( rt PA ) and human single chain urokinase type plasminogen activator ( scu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator does not possess measurable intrinsic amidolytic or plasminogen activator activities . ^^^ The question whether single chain urokinase type plasminogen activator ( Sc uPA ) possesses an enzymatic activity has been a subject of intense investigation for a number of years but still remains unresolved . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A cyclopeptidic suicide substrate preferentially inactivates urokinase type plasminogen activator . c [ Arg aB ( CH2+SCH3 phi ) Gly 4 ] was designed and studied as a mechanism based inactivator ( suicide substrate ) for plasminogen activators ( u PA and t PA ) and plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effect of chemical conjugation of recombinant single chain urokinase type plasminogen activator with monoclonal antiplatelet antibodies on platelet aggregation and on plasma clot lysis in vitro and in vivo . ^^^ The murine monoclonal antiplatelet antibodies MA TSPI 1 ( directed against human thrombospondin ) and MA PMI 2 , MA PMI 1 , and MA LIBS 1 ( directed against ligand induced binding sites [ LIBS ] on human platelet glycoprotein IIb / IIIa ) were conjugated with recombinant single chain urokinase type plasminogen activator ( rscu PA ) using the cross linking reagent N succinimidyl 3 ( 2 pyridyldithio ) propionate ( SPDP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activating activity in plasma from healthy volunteers after infusion of desamino D arginine vasopressin ( DDAVP ) was only partially blocked ( for 77 % ) with specific antibodies to tissue type plasminogen activator and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Secretion of single chain urokinase type plasminogen activator from insect cells . ^^^ A cDNA encoding human urokinase type plasminogen activator was inserted downstream from the polyhedrin promoter of the baculovirus Autographa californica nuclear polyhedrosis virus . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A decreased PAI expressed against tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) was found in lungs of all treated rats compared to controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
FDP D dimer induces the secretion of interleukin 1 , urokinase type plasminogen activator , and plasminogen activator inhibitor 2 in a human promonocytic leukemia cell line . ^^^ In addition , D dimer induced a rapid increase in urokinase type plasminogen activator on day 1 ( 0 . 52 + / 0 . 02 ng / mL 5 0 . 07 + / 0 . 01 ng / mL in the control culture ) and a slow increase in plasminogen activator inhibitor 2 on day 5 ( 3 . 9 + / 1 . 6 ng / mL 5 1 . 2 + / 0 . 2 ng / mL in the control culture ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These results are consistent with the existence of local amplification of expression of enzymatically active plasminogen activators , and particularly of urokinase type plasminogen activator , in situ in primary breast cancer tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cellular receptor for human urokinase type plasminogen activator ( u PAR ) is shown by several independent criteria to be a true member of a family of integral membrane proteins , anchored to the plasma membrane exclusively by a COOH terminal glycosyl phosphatidylinositol moiety . 1 ) Amino acid analysis of u PAR after micropurification by affinity chromatography and N [ 2 hydroxy 1 , 1 bis ( hydroxymethyl ) ethyl ] glycine sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed the presence of 2 3 mol of ethanolamine / mol protein . 2 ) Membrane bound u PAR is efficiently released from the surface of human U 937 cells by trace amounts of purified bacterial phosphatidylinositol specific phospholipase C . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biochemical signals controlling the regulation of macrophage urokinase type plasminogen activator activity by colony stimulating factors and other agents . ^^^ Purified hematopoietic growth factors such as colony stimulating factor 1 ( CSF 1 ) or macrophage CSF , granulocyte macrophage CSF , and interleukin 3 or multi CSF , stimulate the urokinase type plasminogen activator ( u PA ) activity of murine bone marrow derived macrophages ( BMM ) and resident peritoneal macrophages . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have studied the effect of the tumor promotor phorbol myristate acetate ( PMA ) on the level of mRNA for the receptor for urokinase type plasminogen activator ( u PAR ) in the human monocyte like cell line U 937 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Receptor mediated endocytosis of urokinase type plasminogen activator ( u PA ) was characterized with the human keratinocyte cell line NCTC , by both biochemical and ultrastructural methods . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator is expressed in stromal cells and its receptor in cancer cells at invasive foci in human colon adenocarcinomas . ^^^ In this study in situ hybridization methods were used to examine biopsy samples from 13 adenocarcinomas of the colon for the presence of mRNA for the urokinase type plasminogen activator ( u PA ) and its specific cell surface receptor ( u PAR ) . ^^^ Urokinase type plasminogen activator mRNA was not detected in the malignant cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Together with previous findings of urokinase type plasminogen activator and its mRNA being located in fibroblast like cells in the tumor stroma and mRNA for the urokinase receptor in the cancer cells at invasive foci , these results indicate a complex cooperativity among several cell types in regulation of plasminogen activation in colon cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PAI 1 is a physiological inhibitor of both tissue type plasminogen activator and urokinase type plasminogen activator , key enzymes in the initiation of fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Antibody against tissue type plasminogen activator ( t PA ) inhibited the EGF stimulated cell migration in both assays by approximately 70 % , but antibody against urokinase type plasminogen activator ( u PA ) could not inhibit its migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied the substrate specificity , particularly the preference for arginyl over lysyl peptide bonds , of tissue type plasminogen activator ( t PA ) as well as of two chain urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation of human monocytes by granulocyte macrophage colony stimulating factor : increased urokinase type plasminogen activator activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Localization of urokinase type plasminogen activator messenger RNA in the normal mouse by in situ hybridization . ^^^ The histological distribution of urokinase type plasminogen activator ( u PA ) mRNA was analyzed in normal mouse tissue by in situ hybridization with anti sense RNA transcribed from three different subclones of a mouse u PA cDNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mean levels of total plasminogen activator ( total PA ) and urokinase type plasminogen activator ( u PA ) activities in the gastric carcinomas were significantly higher than those in the background normal tissues ( p less than 0 . 001 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Physical exercise induces enhancement of urokinase type plasminogen activator ( u PA ) levels in plasma . ^^^ In this study we have investigated the possible contribution of urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At high seeding density , EGF stimulated an increase in urokinase type plasminogen activator activity , which may have augmented the ability of cells to degrade the extracellular matrix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) antigen , tissue type plasminogen activator ( t PA ) antigen and activity , plasminogen activator inhibitor ( PAI ) type 1 antigen , PAI activity , antithrombin ( AT ) 3 activity , and protein C activity were measured in 24 patients suffering from sepsis or septic shock and the results were compared with those observed in 30 non sepsis patients with severe infectious disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An analysis of the activators of single chain urokinase type plasminogen activator ( scu PA ) in the dextran sulphate euglobulin fraction of normal plasma and of plasmas deficient in factor 12 and prekallikrein . ^^^ An analysis was made of the various possible activators of single chain urokinase type plasminogen activator ( scu PA ) in the dextran sulphate euglobulin fraction ( DEF ) of human plasma . scu PA activators were detected in an assay system in which the substrate scu PA , in physiological concentration ( 50 pM ) , was immuno immobilized . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression and localization of urokinase type plasminogen activator and its type 1 inhibitor are regulated by retinoic acid and fibroblast growth factor in human teratocarcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The recombinant chimeric plasminogen activator , rt PA delta FE / scu PA e , consisting of amino acids 1 to 3 and 87 to 274 of tissue type plasminogen activator ( t PA ) and amino acids 138 to 411 of single chain urokinase type plasminogen activator ( scu PA ) , has a markedly increased thrombolytic potency following its continuous intravenous infusion in animal models of venous thrombosis ( Collen et al , Circulation , in press ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Half life of single chain urokinase type plasminogen activator ( scu PA ) and two chain urokinase type plasminogen activator ( tcu PA ) in patients with acute myocardial infarction . ^^^ The pharmacokinetics of urokinase ( two chain urokinase type plasminogen activator , tcu PA ) and single chain urokinase type plasminogen activator ( scu PA ) were studied in 20 patients with acute myocardial infarction ( AMI ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cellular migration typically requires cell surface associated urokinase type plasminogen activator ( u PA ) and plasminogen , both of which are present as proenzymes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transforming growth factor beta stimulates urokinase type plasminogen activator and DNA synthesis , but not prostaglandin E 2 production , in human synovial fibroblasts . ^^^ Transforming growth factor beta ( TGF beta ) is usually associated with matrix formation and tissue repair ; in contrast , cellular expression of the serine proteinase , urokinase type plasminogen activator ( u PA ) is often correlated with tissue remodeling , as well as with cell migration and transformation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Chimeric molecules comprising the A chain of tissue type plasminogen activator ( t PA ) and the catalytic domain of urokinase type plasminogen activator ( u PA ) have intact enzymatic characteristics of u PA , partial fibrin binding properties of t PA , and thrombolytic properties in animal models comparable with but not superior to those of single chain u PA ( scu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We determined plasma levels of tissue type plasminogen activator ( t PA ) antigen , urokinase type plasminogen activator ( u PA ) antigen , and activity of the fast acting inhibitor of plasminogen activator ( PAI 1 ) in patients with different stages of liver cirrhosis ( Child A , B , and C ) and in age and sex matched healthy controls to investigate the contribution of the liver to the metabolism of these main components of the fibrinolytic system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunolocalization of urokinase type plasminogen activator in adenomas and carcinomas of the colorectum . ^^^ Carcinogenesis in the human colon is associated with a marked increase in the tissue content of the urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Monoclonal antibodies monospecific to single chain urokinase type plasminogen activator ( scu PA ) . ^^^ Until now , single chain urokinase type plasminogen activator ( scu PA ) could not be distinguished from urokinase ( UK ) by immunological methods . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Chymotrypsin , plasmin and kallikrein were inhibited to a lesser extent , but urokinase type plasminogen activator , elastase , thrombin and papain were not inhibited . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistological detection of Saruplase ( recombinant single chain urokinase type plasminogen activator ) in normal rat tissue . ^^^ Saruplase a recombinant single chain urokinase type plasminogen activator was identified immunohistochemically in normal rat tissue after intravenous administration by means of a polyclonal antibody . ^^^ It is discussed that the rat specific receptors for urokinase type plasminogen activator on endothelial cells can not bind Saruplase due to the extreme species specificity . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An Mr 57 , 000 single chain chimeric plasminogen activator , K12G0S32 , consisting of a variable region fragment ( Fv ) derived from the fibrin fragment D dimer specific monoclonal antibody MA 15C5 and of a 33 kDa ( amino acids Ala 132 to Leu 411 ) recombinant single chain urokinase type plasminogen activator ( rscu PA 33k ) was studied . ^^^ The specific activity of both its single chain and two chain forms on fibrin plates was 100 , 000 IU / mg of urokinase type plasminogen activator ( u PA ) equivalent . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Metastatic behavior of human melanoma cell lines in nude mice correlates with urokinase type plasminogen activator , its type 1 inhibitor , and urokinase mediated matrix degradation . ^^^ In three of these five cell lines , this process was mediated by tissue type plasminogen activator ( t PA ) and in the other two cell lines by urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Differential expression of urokinase type plasminogen activator and its type 1 inhibitor during healing of mouse skin wounds . ^^^ The expression of urokinase type plasminogen activator ( u PA ) and its type 1 inhibitor ( PAI 1 ) was examined in vivo in mouse wounds by in situ hybridization and immunohistochemistry . u PA mRNA was present in both basal and suprabasal keratinocytes in the regenerative epithelial outgrowths at the edge of the wounds . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A recombinant plasminogen activator with high fibrin affinity and specificity was expressed by transfecting hybridoma cells with a plasmid that combines sequence coding for low molecular mass ( 32 kDa ) single chain urokinase type plasminogen activator [ scuPA ( 32kDa ) ] and anti fibrin monoclonal antibody 59D8 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We measured antigen levels of two kinds of plasminogen activators , tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( UK ) , as well as their primary inhibitor , type 1 plasminogen activator inhibitor ( PAI 1 ) in the tissue extracts of benign and malignant breast tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Oncostatin M stimulates urokinase type plasminogen activator activity in human synovial fibroblasts . ^^^ Synoviocyte urokinase type plasminogen activator ( u PA ) expression may be relevant to the tissue remodelling , as well as to the cell migration and transformation occurring in rheumatoid joints . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Neoplastic growth and metastatic spread of adenocarcinomas is characterized by a marked increase of urokinase type plasminogen activator ( u PA ) and a decrease of tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Rous sarcoma virus transformed chick embryo fibroblasts ( RSVCEF ) , in contrast to transformed mammalian cells , produce active , two chain urokinase type plasminogen activator ( tcu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Lysosomal degradation of receptor bound urokinase type plasminogen activator is enhanced by its inhibitors in human trophoblastic choriocarcinoma cells . ^^^ We have studied the effect of plasminogen activator inhibitors PAI 1 and PAI 2 on the binding of urokinase type plasminogen activator ( u PA ) to its receptor in the human choriocarcinoma cell line JAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation with single chain urokinase type plasminogen activator ( scu PA ) . ^^^ The mechanism of the activation of plasminogen by single chain urokinase type plasminogen activator ( single chain u PA , scu PA ) was studied using rscu PA Glu 158 , a recombinant plasmin resistant mutant of human scu PA obtained by site specific mutagenesis of Lys 158 to Glu , and rPlg Ala 740 , a recombinant human plasminogen in which the catalytic site is destroyed by mutagenesis of the active site Ser 740 to Ala . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Soluble factors released by the target organ enhance the urokinase type plasminogen activator activity of metastatic tumor cells . ^^^ Many studies suggest that urokinase type plasminogen activator ( u PA ) has a significant role in several steps of the metastatic process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Taprostene , a stable prostacyclin analogue , enhances the thrombolytic efficacy of saruplase ( recombinant single chain urokinase type plasminogen activator ) in rabbits with pulmonary embolized thrombi . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical evidence of urokinase type plasminogen activator in primary and metastatic tumors of pulmonary adenocarcinoma . ^^^ The urokinase type plasminogen activator ( u PA ) was used to study 96 cases of lung adenocarcinoma and 49 cases of lymph node metastatic adenocarcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Role of alpha 2 antiplasmin in fibrin specific clot lysis with single chain urokinase type plasminogen activator in human plasma . ^^^ The role of plasma alpha 2 antiplasmin ( alpha 2 AP ) in the fibrin specificity of clot lysis by recombinant single chain urokinase type plasminogen activator ( rscu PA ) and in the conversion of rscu PA to its two chain derivative ( rtcu PA , urokinase ) was investigated in an in vitro human plasma clot lysis system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of plasminogen to preformed human plasma clots immersed in citrated human plasma was measured and correlated with the sensitivity of these clots to lysis with recombinant tissue type plasminogen activator ( rt PA ) , recombinant single chain urokinase type plasminogen activator ( rscu PA ) or two chain urokinase type plasminogen activator ( tcu PA , urokinase ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Intravenous thrombolytic therapy with a combination of single chain urokinase type plasminogen activator and recombinant tissue type plasminogen activator in acute myocardial infarction . ^^^ The effects of simultaneous intravenous infusions of 12 mg recombinant tissue type plasminogen activator ( rt PA ) over 30 minutes and 48 mg single chain urokinase type plasminogen activator ( scuPA ) over 40 minutes were studied in 38 patients with acute myocardial infarction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effect of fibrin targeting on clot lysis with urokinase type plasminogen activator . ^^^ The effect of fibrin targeting of urokinase type plasminogen activator ( u PA ) on its fibrinolytic potency was studied using recombinant fusion proteins of u PA with the NH 2 terminal region of tissue type plasminogen activator ( t PA / u PA ) and chemical complexes of u PA with F ( ab ' ) 2 fragments of a fibrin specific monoclonal antibody ( u PA / MA 15C5 F ( ab ' ) 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator is increased in the involuting ventral prostate of castrated rats . ^^^ Sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS PAGE ) and zymography demonstrated two major Mr forms of plasminogen activators that were found to be strongly increased by castration ; inclusion of quenching antibodies in the zymography and immunoblotting analysis identified these as urokinase type plasminogen activator ( u PA ) and its Mr 30 , 000 degradation product , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Properties of urokinase type plasminogen activator found in chest fluid . ^^^ Urokinase type plasminogen activator ( u PA ) was purified from three different chest fluids obtained from patients with liver cirrhosis and pleuritis , aplastic anemia and pneumonia , and lung tumor , and the relationship between molecular weight and plasminogen activator ( PA ) activity was examined by zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The tissue specific distribution of tissue type and urokinase type plasminogen activator ( t PA and u PA ) and their inhibitor type 1 ( PAI 1 ) was analyzed at mRNA level in five major rat organ tissues . t PA mRNA was detected in lung , kidney , heart , and liver . u PA mRNA was detected in kidney and lung . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparison of intravenous bolus injection or continuous infusion of recombinant single chain urokinase type plasminogen activator ( saruplase ) for thrombolysis . ^^^ The thrombolytic efficacy of recombinant unglycosylated full length single chain urokinase type plasminogen activator ( rscu PA , saruplase ) , applied either as single intravenous bolus or as a continuous infusion over 60 minutes , was studied in 5 randomized blinded groups of 5 dogs with combined copper coil induced coronary artery thrombosis and 125I fibrin labeled femoral vein clots . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) , tumour necrosis factor alpha ( TNF alpha ) and interleukin 1 ( IL 1 ) activities were measured for highly purified human monocytes cultured for 18 hr with recombinant human interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombolytic and pharmacokinetic properties of a conjugate of recombinant single chain urokinase type plasminogen activator with a monoclonal antibody specific for cross linked fibrin in a baboon venous thrombosis model . ^^^ Chemical conjugates between recombinant single chain urokinase type plasminogen activator ( rscu PA ) and a murine monoclonal antibody directed against fragment D dimer of cross linked human fibrin ( MA 15C5 ) , rscu PA / MA 15C5 , and between rscu PA and a control monoclonal antibody ( MA 1C8 ) , rscu PA / MA 1C8 , were produced by cross linking with N succinimidyl 3 ( 2 pyridyldithio ) propionate ( SPDP ) . ^^^ In an in vitro system composed of a [ 125 1 ] fibrin labeled baboon plasma clot immersed in autologous citrated plasma , dose and time dependent lysis was obtained with a ratio of the potencies of free and conjugated rscu PA similar to that in human plasma : 50 % lysis in 2 hours required 4 . 3 micrograms / ml rscu PA , 1 . 0 microgram / ml urokinase type plasminogen activator ( u PA ) equivalent rscu PA / MA 15C5 , or 15 micrograms / ml u PA equivalent rscu PA / MA 1C8 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Phorbol myristate acetate ( PMA ) which mimics the effect of DAG on PKC induces transcriptional activation of the urokinase type plasminogen activator ( u PA ) gene in LLC PK 1 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Localization of urokinase type plasminogen activator in human eyes : an immunocytochemical study . ^^^ We investigated the presence of urokinase type plasminogen activator ( u PA ) in various structures of the human eye by using a biotin strepavidin complex immunoperoxidase technique with a monoclonal antibody specific for human u PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These data show that normal rat pleural leukocytes contain plasminogen activator inside the cells and synthesize a urokinase type plasminogen activator inhibitor in culture that may be important in the fibrinolysis / coagulation balance in the pleural space . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Peritubular cells secrete urokinase type plasminogen activator ( u PA ) and exhibit slight degrading activity at 86 kDa and 74 kDa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Apart from tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) , a third PA appears to occur in human plasma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These include streptokinase , urokinase , recombinant tissue type plasminogen activator ( rt PA ) , anisoylated plasminogen streptokinase activator complex ( APSAC ) and single chain urokinase type plasminogen activator ( scu PA , prourokinase ) . ^^^ Single chain urokinase type plasminogen activator is more fibrin specific than urokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Time dependent activation by HuTSP 1 of recombinant human pro urokinase as well as natural pro urokinase derived from human melanoma cells was demonstrated in a chromogenic assay specific for active urokinase type plasminogen activator and in immunoblotting experiments revealing the conversion of single chain into two chain urokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prourokinase activation on the surface of human rhabdomyosarcoma cells : localization and inactivation of newly formed urokinase type plasminogen activator by recombinant class 2 plasminogen activator inhibitor . ^^^ Recombinant class 2 plasminogen activator inhibitor ( PAI 2 ) was used in an approach to probe the formation and location of enzymatically active urokinase type plasminogen activator ( u PA ) sites on the surface of cultured human rhabdomyosarcoma cells ( RD cells ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor necrosis factor induces the production of urokinase type plasminogen activator by human endothelial cells . ^^^ Primary cultures of human endothelial cells release very little urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both analogues were as potent as [ Arg 8 ] vasopressin in stimulating the cAMP dependent protein kinase and the production of urokinase type plasminogen activator in LLC PK 1 cells , with concentration dependence consistent with receptor binding affinities . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two human breast epithelial cell lines ( HBL 100 , HMT 3522 ) of non malignant origin and six ( MCF 7 , T 47 D , ZR 75 1 , CAMA 1 , BT 20 , HMT 3909 ) of malignant origin have been studied to evaluate whether in vitro invasion and / or secretion of urokinase type plasminogen activator are related to tumorigenicity in athymic nude mice . ^^^ Secretion of urokinase type plasminogen activator was neither correlated to tumorigenicity nor to invasion in vitro nor to malignancy of tissue origin . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of thrombin and plasmin resistant mutants of recombinant human single chain urokinase type plasminogen activator . ^^^ Recombinant human single chain urokinase type plasminogen activator ( suc PA ) ( SM 0 : wild type ) and its variants resistant to plasmin and / or thrombin ( SM 1 : Lys 135 to Gln ; SM 3 : Phe 157 to Asp ; and SM 4 : Lys 135 to Gln and Phe 157 to Asp ) have been constructed by site directed mutagenesis with the aim of producing more efficient thrombolytic agents [ Miyake , T . et al . ( 1988 ) J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of domain deletion and / or duplication mutants of a recombinant chimera of tissue type plasminogen activator and urokinase type plasminogen activator ( rt PA / u PA ) . ^^^ Chimeric molecules comprising the A chain of tissue type plasminogen activator ( t PA ) and the catalytic domain of urokinase type plasminogen activator ( u PA ) have intact enzymatic characteristics of u PA , but only partial fibrin binding properties of t PA ( Nelles et al . , J Biol Chem 1987 ; 262 : 10855 62 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Localization of urokinase type plasminogen activator receptor on U 937 cells : phorbol ester PMA induces heterogeneity . ^^^ The binding of human urokinase type plasminogen activator ( u PA ) to the surface of the human monocytic cell line U 937 was studied by immunological detection of bound u PA or binding of biotinylated diisopropyl fluorophosphate inactivated human u PA visualized by light or electron microscopy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for human urokinase type plasminogen activator ( u PA ) was purified from phorbol 12 myristate 13 acetate stimulated U 937 cells by temperature induced phase separation of detergent extracts , followed by affinity chromatography with immobilized diisopropyl fluorophosphate treated u PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Macromolecular interaction on a cAMP responsive region in the urokinase type plasminogen activator gene : a role of protein phosphorylation . ^^^ We have studied the regulation of urokinase type plasminogen activator gene expression by cAMP in LLC PK 1 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stable transfection of three human tumor cell lines H ras transformed bronchial epithelial cells TBE 1 , fibrosarcoma cells HT 1080 , and melanoma cells A 2058 with the adenovirus E1A gene dramatically repressed the expression of the secreted proteases , type 4 and interstitial collagenases , and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two murine monoclonal antibodies produced against human urokinase type plasminogen activator were characterized with respect to their antigen binding specificity and their effects on urokinase activity and urokinase receptor binding . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mutants so selected were then characterized using a novel screening system for detection of the production of urokinase type plasminogen activator in response to cAMP agonists . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These include streptokinase , urokinase , recombinant tissue type plasminogen activator ( rt PA ) , anisoylated plasminogen streptokinase activator complex ( APSAC ) and single chain urokinase type plasminogen activator ( scu PA , prourokinase ) . ^^^ Single chain urokinase type plasminogen activator is more fibrin specific than urokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The agents that have been used in humans thus far include streptokinase and urokinase , as well as tissue type plasminogen activator and , most recently , single chain urokinase type plasminogen activator or pro urokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and single chain urokinase type plasminogen activator ( scu PA , prourokinase ) are more fibrin specific ; however , at the large dosages of activator needed for therapeutic efficacy , bleeding complications are still a problem . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human non small lung cancer cell lines HS 24 ( established from a primary squamous cell carcinoma ) and SB 3 ( established from a metastasis of a primary adenocarcinoma of the lung into the adrenal gland ) were analysed for the proteinases tissue type plasminogen activator ( tPA ) , urokinase type plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator levels and PA inhibitor levels did not reveal any significant differences between the different groups . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cells of the monocyte / macrophage lineage are known to produce urokinase type plasminogen activator ( u PA ) and are active participants in the inflammatory response . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The Mr = 50 , 000 species co migrates with urokinase type plasminogen activator . ^^^ Studies with antibodies also indicate that the activity produced is urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interestingly , several other markers of acute inflammation such as prostaglandin E 2 ( PGE 2 ) , C3a , urokinase type plasminogen activator ( U PA ) , which were found to be significantly elevated in the BAL of the same group of subjects ( 18 h post exposure ) , were not elevated in the NAL either immediately post or 18 h post exposure . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The PAI inhibited not only urokinase type plasminogen activator ( u PA ) but single and two chain tissue type plasminogen activators ( t PAs ) on plasminogen containing fibrin plate . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
All End cell lines examined expressed high levels of fibrinolytic activity resulting from increased production of urokinase type plasminogen activator and decreased production of plasminogen activator inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The thrombolytic agents currently in use or being extensively evaluated include streptokinase , urokinase , tissue type plasminogen activator ( t PA ) , anisoylated plasminogen streptokinase activator complex ( APSAC or anistreplase ) , and single chain urokinase type plasminogen activator ( scu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We also show that basic FGF concomitantly stimulates endothelial cells to produce a urokinase type plasminogen activator , a protease that has been implicated in the neovascular response . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Coronary thrombolysis with recombinant single chain urokinase type plasminogen activator in patients with acute myocardial infarction . ^^^ Seventeen patients with acute transmural myocardial infarction and angiographically confirmed complete coronary occlusion were treated with heparin combined with intravenous single chain urokinase type plasminogen activator ( scu PA ) , obtained by expression of the cDNA encoding mature human scu PA in Escherichia coli . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombolysis with recombinant human single chain urokinase type plasminogen activator ( rscu PA ) : dose response in dogs with coronary artery thrombosis . ^^^ The dose response of an intravenous i . v . infusion for 30 min of recombinant human single chain urokinase type plasminogen activator ( rscu PA ) was investigated in dogs with 1 h old clots in the left anterior descending coronary artery . ^^^ It is concluded that i . v . infusion of recombinant single chain urokinase type plasminogen activator ( rscu PA ) at a sufficiently high rate ( greater than or equal to 8 micrograms / kg / min ) produces coronary thrombolysis without systemic fibrinolysis in dogs . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Migrating keratinocytes express urokinase type plasminogen activator . ^^^ Using a rabbit antibody against urokinase type plasminogen activator ( u PA ) and an avidin biotin peroxidase detection method , we localized u PA in the keratinocytes at the leading edge of the migrating cultures . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effects of exogenously added urokinase type plasminogen activator , tissue type plasminogen activator , plasmin and thrombin on the proliferation of primary cultures of cells derived from prostatic hyperplasia or prostatic carcinomas were investigated by measuring the incorporation of 3H thymidine into the cultures . ^^^ Addition of urokinase type plasminogen activator ( 1 . 35 10 10 ( 9 ) M ) or thrombin ( 10 ( 7 ) M ) to the culture medium caused a two fold increase of 3H thymidine incorporation , regardless of the origin of the prostatic cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recombinant single chain urokinase type plasminogen activator during acute myocardial infarction . ^^^ Recombinant single chain urokinase type plasminogen activator was intravenously administered in 2 different doses in 24 patients with acute myocardial infarction and angiographically proved occlusion of the infarct related artery . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fifty four patients with Q wave acute myocardial infarction ( AMI ) were treated with heparin combined with intravenous single chain urokinase type plasminogen activator ( prourokinase ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) has a high affinity for fibrin , which is in contrast to urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stimulation of plasmin catalyzed conversion of single chain to two chain urokinase type plasminogen activator by sulfated polysaccharides . ^^^ Influence of heparin , chondroitin sulfate C and dextran sulfate ( MW 3 , 500 and 7 , 000 ) on plasmin catalyzed conversion of single chain ( scu PA ) to two chain ( u PA ) urokinase type plasminogen activator and generation of plasmin in mixtures of scu PA and Glu plasminogen ( Glu plg ) was investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of urokinase type plasminogen activator ( u PA ) in capillary growth was investigated using cultured bovine endothelial cells ( BCE ) on type 1 collagen gels and analyzed by morphometry for quantitative assessment of angiogenesis in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This line has the following biological characters . 1 ) Spontaneous lung metastases are made in nude mice inoculated subcutaneously . 2 ) This line can produce some kinds of proteases , like as tissue type plasminogen activator , urokinase type plasminogen activator and collagenase . 3 ) KUM . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Corneal epithelial cells secrete tissue plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and their inhibitor ( PAI ) , whereas these cell types in other tissues are known to secrete only u PA hitherto . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrin autography revealed that cultured bovine corneal endothelial cells released into the conditioned medium tissue plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Evaluation of fibrinolytic capacity in plasma during thrombolytic therapy with single ( scu PA ) or two chain urokinase type plasminogen activator ( tcu PA ) by a combined assay system for urokinase type plasminogen activator antigen and function . ^^^ A combined assay for urokinase type plasminogen activator ( u PA ) activity and antigen determination in plasma samples is described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When these homotypic labeled myotube matrices were incubated with myoblast conditioned media containing high specific activity urokinase type plasminogen activator , slow , but clearly detectable , release of label occurred . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In resection tissue samples of colorectal carcinomas , the concentration of urokinase type plasminogen activator ( u Pa ) was found to be significantly higher than in the normal parent mucosal tissue , while there was less tissue type plasminogen activator ( t PA ) . u PA and t PA were also determined in endoscopic biopsies of colonic and gastric carcinomas , and the results were compared with those of the ultimate resection samples of the same patients , and with the histological evaluation of adjacent biopsies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Potentiation by Lys plasminogen of clot lysis by single or two chain urokinase type plasminogen activator or tissue type plasminogen activator . ^^^ Study has been made of the influence of addition of human NH 2 terminal glutamic acid plasminogen ( Glu Plg ) or human NH 2 terminal lysine plasminogen ( Lys Plg ) to normal citrated plasma upon the rate of lysis of fully crosslinked plasma clots in the presence of single or two chain urokinase type plasminogen activator ( scu PA / tcu PA ) or tissue plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
DDAVP induces systemic release of urokinase type plasminogen activator . ^^^ The observation of concurrent release of urokinase type plasminogen activator ( u PA ) , which eventually might cooperate in the enhanced fibrinolytic activity , has not been reported thus far . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The study of plasminogen activator in renal cell carcinoma with special remarks on urokinase type plasminogen activator ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human chromosomal DNA encoding single chain urokinase type Plasminogen Activator ( scu PA , or pro urokinase ) was inserted in an expression plasmid and transfected in human A 431 , mouse LB 6 and CHO cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Additive fibrinolysis by recombinant tissue type plasminogen activator ( r t PA ) and recombinant single chain urokinase type plasminogen activator ( r scu PA ) in rabbit pulmonary thrombosis . ^^^ The mode of interaction between recombinant tissue type plasminogen activator ( r t PA ) and recombinant single chain urokinase type plasminogen activator ( r scu PA ) has been investigated in in vivo experiments . 125J fibrin labeled clots were embolized via a jugular vein into the lungs of anesthetized rabbits . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect was studied with plasma clots before or after mechanical compression and with whole blood clots before or after retraction , using either the fibrin specific plasminogen activators recombinant tissue type plasminogen activator ( rt PA ) or recombinant single chain urokinase type plasminogen activator ( rscu PA ) , and the non fibrin specific activators recombinant two chain urokinase type plasminogen activator ( rtcu PA ) , or streptokinase ( SK ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The RH PA cell line a source for obtaining the urokinase type plasminogen activator ] . ^^^ The new line produces urokinase type plasminogen activator ( PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enhanced thrombolysis in plasma and in vivo by single chain urokinase type plasminogen activator ( scuPA ) conjugated to an antifibrin antibody . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
New thrombolytic agents , such as tissue plasminogen activator ( tPA ) , single chain urokinase type Plasminogen Activator ( scuPA ) and Anisoylated Plasminogen Streptokinase Activator Complex ( APSAC ) have been evaluated in clinical trials . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biochemical properties of conjugates of urokinase type plasminogen activator with a monoclonal antibody specific for cross linked fibrin . ^^^ Equimolar mixtures of recombinant single chain urokinase type plasminogen activator ( rscu PA ) and a murine monoclonal antibody ( MA 15C5 ) directed against fragment D dimer of human cross linked fibrin were conjugated , using the cross linking agent N succinimidyl 3 ( 2 pyridyldithio ) propionate ( PySSProSu ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Assay method for single chain urokinase type plasminogen activator . ^^^ A new assay method for measuring single chain urokinase type plasminogen activator ( scu PA ) activity by quantitative calorimetric assay method is described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding was not inhibited by fibrinogen or single chain urokinase type plasminogen activator , but plasminogen partially competed for binding of 125I rt PA to platelets ( up to 40 % displacement ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied human glomerular epithelial cells in culture which were found to release mainly a urokinase type plasminogen activator ( u PA ) identified on zymography by its molecular weight ( 53 kD ) , its plasminogen activator activity , and its neutralization by specific polyclonal anti u PA IgG . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Several human lung tumor cell lines derived from large cell , squamous cell , and small cell carcinomas , as well as from mesotheliomas of the lung have been investigated for their gene expression and secretion of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitors 1 and 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When the truncal neural tube was divided in four equal segments , the secretion pattern of the two types of plasminogen activators was similar for the cells from the three most anterior segments ; cells having migrated from the most caudal one , i . e . consisting of the neural plate , secreted a higher level of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In LLC PK 1 porcine epithelial cells , the urokinase type plasminogen activator ( u PA ) mRNA and protein can be induced either by stimulation of the protein kinase C ( PKC ) pathway using a tumor promoter ( PMA ) or by stimulation of the protein kinase A ( PKA ) pathway with calcitonin ( SCT ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Five thrombolytic agents are either available or under clinical investigation : streptokinase ( SK ) , urokinase ( UK ) , recombinant tissue type plasminogen activator ( rt PA ) , anisoylated plasminogen streptokinase activator complex ( APSAC ) and single chain urokinase type plasminogen activator ( scu PA , pro urokinase ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In colon mucosa the percentage of t PA to urokinase type plasminogen activator ( u PA ) was 80 : 20 % in the control group and 71 : 29 % in the IBD group in non inflamed mucosa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Placental microvillous membranes exhibited saturable binding of urokinase type plasminogen activator with plateau achieved by 30 min at 4 degrees C and 10 min at 37 degrees C . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A 55 , 000 60 , 000 Mr receptor protein for urokinase type plasminogen activator . ^^^ The iodinated Mr approximately equal to 15 , 000 amino terminal fragment ( ATF ) of the urokinase type plasminogen activator ( u PA ) molecule bound specifically to the cell surface of all of seven cultured human tumor cell lines studied . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Potentiation by heparin fragments of thrombolysis induced with human tissue type plasminogen activator or human single chain urokinase type plasminogen activator . ^^^ San Francisco , CA ) or human single chain urokinase type plasminogen activator ( scu PA , Sandoz AG , Basle , Switzerland ) was measured in a rabbit jugular vein thrombosis model . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast , the accumulation of urokinase type plasminogen activator mRNA is observed in only a few of the tissues analyzed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enzymatic properties of single chain and two chain forms of a Lys 158 Glu 158 mutant of urokinase type plasminogen activator . ^^^ Recombinant single chain urokinase type plasminogen activator ( rscu PA ) , in which the plasmin sensitive peptide bond Lys 158 Ile159 is destroyed by site specific mutagenesis of Lys 158 to Glu ( rscu PA Glu 158 ) , is quantitatively converted to two chain urokinase type plasminogen activator ( rtcu PA Glu 158 ) by treatment with endoproteinase Glu C ( Staphylococcus aureus V 8 proteinase ) . ^^^ It is concluded that the amino acid in position 158 is a main determinant of the functional properties of single chain urokinase type plasminogen activator but not of the two chain form . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Definitive demonstration of a role for plasmin in activating the proenzyme of urokinase type plasminogen activator . ^^^ Selective use of these monoclonal antibodies demonstrated unequivocally that plasmin mediates the activation of the proenzyme form of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stimulation by heparin of the plasmin mediated conversion of single chain to two chain urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Degradation of glycoprotein and collagenous components of the basement membrane : studies with urokinase type plasminogen activator , alpha thrombin , and plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Whereas crude bovine thrombin activated single chain urokinase type plasminogen activator ( scu PA ) , otherwise called pro urokinase ( pro UK ) , purified human thrombin converted pro UK ( scu PA ) to a two chain form that had no amidolytic activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of a fusion protein consisting of amino acids 1 to 263 of tissue type plasminogen activator and amino acids 144 to 411 of urokinase type plasminogen activator . ^^^ A hybrid human cDNA was constructed by splicing of a cDNA fragment of tissue type plasminogen activator ( t PA ) , encoding 5 ' untranslated , the pre pro region and amino acids Ser 1 Thr263 , with a cDNA fragment of urokinase type plasminogen activator ( u PA ) , encoding amino acids Leu 144 Leu411 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The only thrombolytic agents with a relative fibrin specificity available for clinical purposes are tissue type plasminogen activator and single chain urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation with plasmin of tow chain urokinase type plasminogen activator derived from single chain urokinase type plasminogen activator by treatment with thrombin . ^^^ Thrombin converts single chain urokinase type plasminogen activator ( scu PA ) to an inactive two chain derivative ( thrombin derived tcu PA ) by hydrolysis of the Arg 156 Phe 157 peptide bond . ^^^ In the present study , we show that inactive thrombin derived tcu PA ( specific activity 1000 IU / mg ) can be converted with plasmin to active two chain urokinase type plasminogen activator ( specific activity 43 , 000 IU / mg ) by hydrolysis of the Lys 158 Ile 159 peptide bond . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proenzyme to urokinase type plasminogen activator in human colon cancer : in vitro inhibition by monocyte minactivin after proteolytic activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biochemical and thrombolytic properties of a low molecular weight form ( comprising Leu 144 through Leu 411 ) of recombinant single chain urokinase type plasminogen activator . ^^^ The cDNA encoding a low Mr derivative ( residues 144 411 ) of human single chain urokinase type plasminogen activator was cloned , the recombinant low Mr single chain urokinase type plasminogen activator ( rscu PA 32k ) was expressed in Chinese hamster ovary cells , and the translation product was purified to homogeneity from conditioned cell culture medium . rscu PA 32k is very similar to intact recombinant single chain urokinase type plasminogen activator in terms of its very low activity ( 120 IU / mg ) on a chromogenic substrate for urokinase ( pyroglutamylglycylarginine p nitroanilide ) , its plasminogen dependent fibrinolytic activity on fibrin plates ( specific activity = 170 , 000 IU / mg ) , its plasminogen activating potential , and the lack of specific binding to fibrin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One chain urokinase type plasminogen activator from human sarcoma cells is a proenzyme with little or no intrinsic activity . ^^^ We have compared the plasminogen activating capacity of one and two chain urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cloning , nucleotide sequencing and expression of cDNAs encoding mouse urokinase type plasminogen activator . ^^^ As a step in the study of the expression of these enzymes in mouse tissues , we have isolated five cDNAs encoding the mouse urokinase type plasminogen activator from a cDNA library prepared with size selected mRNA from MSV transformed 3T3 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have studied the regulation by glucocorticoids and dibutyryl cAMP of the amounts of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) and a Mr approximately 54000 plasminogen activator inhibitor accumulated in serum free conditioned culture fluid by a human fibrosarcoma , a human glioblastoma and a human melanoma cell line ( HT 1080 , UCT / gl 1 and Bowes ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation is mediated by plasminogen activators which are classified as either tissue type plasminogen activator ( t PA ) or urokinase type plasminogen activator ( u PA ) . t PA and single chain u PA ( scu PA ) induce clot specific thrombolysis , however via entirely different mechanisms . t PA is relatively inactive in the absence of fibrin , but fibrin strikingly enhances the activation rate of plasminogen by t PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Consequently , the designation `` single chain urokinase type plasminogen activator ' ' was recently adopted by the International Committee on Thrombosis and Haemostasis ( Annual Meeting , San Diego , CA , July 13 14 , 1985 ) . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Purification and characterization of single chain urokinase type plasminogen activator from human cell cultures . ^^^ A urokinase type plasminogen activator was purified from conditioned media of several human cell cultures , but preferably from the human lung adenocarcinoma line CALU 3 ( ATCC , HTB 55 ) , using a combination of chromatography on zinc chelate Sepharose , SP Sephadex C 50 , and Sephadex G 100 . ^^^ The purified plasminogen activator consists of a single polypeptide chain with Mr 54 , 000 as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and is very similar or identical to single chain urokinase type plasminogen activator on the basis of immunodiffusion , amino acid composition , and the lack of specific binding to fibrin . ^^^ It is concluded that the plasminogen activator purified from CALU 3 conditioned media is physically and kinetically identical to single chain urokinase type plasminogen activator . ^^^ With the present straightforward purification method and a readily available source , sufficient amounts of single chain urokinase type plasminogen activator can be obtained for more detailed investigations of its biochemical , biological , and thrombolytic properties . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Coronary thrombolysis with human single chain , urokinase type plasminogen activator ( pro urokinase ) in patients with acute myocardial infarction . ^^^ Single chain , urokinase type plasminogen activator ( pro urokinase ) , isolated from the conditioned medium of a human renal adenocarcinoma cell line , was administered to six patients with evolving myocardial infarction of less than 5 hours ' duration and angiographically confirmed total occlusion of the infarct related coronary artery . ^^^ Single chain , urokinase type plasminogen activator can induce clot selective coronary thrombolysis in patients with acute myocardial infarction . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Serine proteinase inhibitors and a monoclonal antibody against urokinase type plasminogen activator inhibited the conversion , indicating that it was caused by plasminogen activator catalyzed proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Highly purified pro urokinase ( pro UK ) or single chain urokinase type plasminogen activator ( scu PA ) was treated with diisopropylfluorophosphate ( 1 mmol / L ) to eliminate traces of two chain UK activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It resembles urokinase type plasminogen activator in its inability to be stimulated by fibrin and its dose response on human fibrin plates . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor from human fibrosarcoma cells binds urokinase type plasminogen activator , but not its proenzyme . ^^^ The inhibitor inhibited human urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator , but not plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Coronary thrombolysis by intravenous infusion of recombinant single chain urokinase type plasminogen activator or recombinant urokinase in baboons : effect on regional blood flow , infarct size and hemostasis . ^^^ In six animals , single chain urokinase type plasminogen activator , obtained by recombinant deoxyribonucleic acid ( DNA ) technology , was infused intravenously at a rate of 20 micrograms / kg per min for 60 minutes after approximately 45 minutes of coronary thrombosis . ^^^ The infusion of recombinant single chain urokinase type plasminogen activator was not associated with systemic activation of the fibrinolytic system , fibrinogen breakdown or evident bleeding . ^^^ Persistent reperfusion occurred within 14 + / 5 minutes ( p less than 0 . 05 versus recombinant single chain urokinase type plasminogen activator ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Identification and localization of urokinase type plasminogen activator in human NK cells . ^^^ After immunofluorescence staining with polyclonal antiserum against urokinase type plasminogen activator , the majority of LGLs showed granular staining which was located in Golgiderived vesicles . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase inhibitor was inactive towards single chain urokinase type plasminogen activator and plasmin , but it inhibited two chain tissue type plasminogen activator with a k below 10 ( 3 ) M 1 s 1 and thrombin with a k of 4 10 10 ( 4 ) M 1 s 1 in the absence and 2 10 10 ( 5 ) M 1 s 1 in the presence of heparin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enzyme linked immunosorbent assay for human urokinase type plasminogen activator and its proenzyme using a combination of monoclonal and polyclonal antibodies . ^^^ A sensitive and specific enzyme linked immunosorbent assay ( ELISA ) for human urokinase type plasminogen activator ( u PA ) and its inactive proenzyme ( pro u PA ) was developed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The existence of significant synergism between tissue type plasminogen activator ( t PA ) and single chain urokinase type plasminogen activator ( scu PA ) , and between t PA and urokinase in thrombolysis in vivo is described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Absence of synergism between tissue type plasminogen activator ( t PA ) , single chain urokinase type plasminogen activator ( scu PA ) and urokinase on clot lysis in a plasma milieu in vitro . ^^^ A potential synergic effect of tissue type plasminogen activator ( t PA ) , single chain urokinase type plasminogen activator ( scu PA ) or urokinase on clot lysis was investigated in a whole human plasma system in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Purification and characterization of a novel low molecular weight form of single chain urokinase type plasminogen activator . ^^^ A low Mr form ( Mr 32 , 000 ) of single chain urokinase type plasminogen activator ( scu PA ) was isolated from conditioned culture medium of a human lung adenocarcinoma cell line , CALU 3 ( ATCC , HTB 55 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the interim , more fibrin specific thrombolytic agents have been developed such as acylated streptokinase human plasminogen complex , tissue type plasminogen activator ( t PA ) and single chain urokinase type plasminogen activator ( scu PA or pro urokinase ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Exposure of mouse resident and thioglycollate elicited peritoneal macrophages to IFN gamma leads to a marked increase in the TNF alpha ( tumor necrosis factor / cachectin ) , IL 1 and u PA ( urokinase type plasminogen activator ) mRNA levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparative thrombolytic properties of single chain forms of urokinase type plasminogen activator . ^^^ The specific thrombolytic properties of urokinase and three molecular forms of single chain urokinase type plasminogen activator ( scu PA ) were compared in a human plasma milieu in vitro and in an experimental thrombosis model in rabbits . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Phorbol ester induces the biosynthesis of glycosylated and nonglycosylated plasminogen activator inhibitor 2 in high excess over urokinase type plasminogen activator in human U 937 lymphoma cells . ^^^ Immunol . , 71 : 215 223 ) and concomitantly stimulates the biosynthesis of plasminogen activator inhibitor 2 ( PAI 2 ) and of urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Distinct localizations of urokinase type plasminogen activator and its type 1 inhibitor under cultured human fibroblasts and sarcoma cells . ^^^ We studied the immunocytochemical localization of urokinase type plasminogen activator ( u PA ) and the type 1 plasminogen activator inhibitor ( PAI 1 ) in human fibroblasts and sarcoma cells , using both polyclonal and monoclonal antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and single chain urokinase type plasminogen activator ( scu PA ) : potential for fibrin specific thrombolytic therapy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Differential detection of single chain and two chain urokinase type plasminogen activator by a new immunoadsorbent amidolytic assay ( IAA ) . ^^^ A new immunoadsorbent amidolytic assay ( IAA ) for the specific differential detection of two chain urokinase type plasminogen activator ( tcu PA ) and its single chain precursor ( scu PA ) in cell culture supernatants has been developed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Measurement of urokinase type plasminogen activator ( u PA ) with an enzyme linked immunosorbent assay ( ELISA ) based on three murine monoclonal antibodies . ^^^ An enzyme linked immunosorbent assay ( ELISA ) for the measurement of urokinase type plasminogen activator ( u PA ) was developed . ^^^ Three murine monoclonal antibodies to single chain urokinase type plasminogen activator ( scu PA ) were isolated and shown to react with non overlapping epitopes in scu PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Amiloride selectively inhibits the urokinase type plasminogen activator . ^^^ The diuretic drug amiloride , an inhibitor of Na+ uptake , competitively inhibits the catalytic activity of the urokinase type plasminogen activator ( u PA ) , with a Ki of 7 10 10 ( 6 ) M . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of recombinant human single chain urokinase type plasminogen activator mutants produced by site specific mutagenesis of lysine 158 . ^^^ The cDNA encoding full length single chain urokinase type plasminogen activator ( scu PA ) was cloned and sequenced , and the recombinant scu PA ( rscu PA ) was expressed in Chinese hamster ovary cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Lysis did not occur in absence of plasminogen or in presence of antibodies against the urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cholera toxin ( CT ) , dexamethasone ( dex ) , interferon gamma ( IFN gamma ) , concanavalin A ( Con A ) , and endotoxin ( LPS ) induced changes in mRNA levels and transcription rates of both urokinase type plasminogen activator and tumor necrosis factor / cachectin genes , the products of which are sensitive indices of macrophage activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Antibodies against the urokinase type plasminogen activator did not react with any elements of developing glomeruli ; rather , they stained the proximal tubules in more mature parts of the kidney , as revealed by double immunostaining using antibodies against the brush border . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mutant urokinase type plasminogen activator ( u PA ) genes and hybrid genes between tissue type plasminogen activator ( t PA ) and u PA have been designed to direct the synthesis of new plasminogen activators and to investigate the structure function relationship in these molecules . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of a recombinant fusion protein of the finger domain of tissue type plasminogen activator with a truncated single chain urokinase type plasminogen activator . ^^^ Human recombinant single chain urokinase type plasminogen activator ( recombinant scu PA ) and a hybrid between human tissue type plasminogen activator ( t PA ) and scu PA , obtained by ligation of cDNA fragments encoding the NH 2 terminal region ( amino acids 1 67 ) of t PA and the COOH terminal region ( amino acids 136 411 ) of scu PA , were expressed in a mammalian cell system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Coronary arterial thrombolysis with low dose synergistic combinations of recombinant tissue type plasminogen activator ( rt PA ) and recombinant single chain urokinase type plasminogen activator ( rscu PA ) for acute myocardial infarction . ^^^ The effect of combined intravenous infusion of 10 mg of recombinant tissue type plasminogen activator ( rt PA ) and 10 mg of recombinant single chain urokinase type plasminogen activator over 1 hour on coronary artery recanalization and on the blood fibrinolytic system was studied in 9 patients with acute myocardial infarction and angiographically confirmed coronary artery occlusion . ^^^ Thus , combined intravenous infusion of rt PA and recombinant single chain urokinase type plasminogen activator induces fibrin specific coronary thrombolysis at approximately one fourth of the dose currently used for each agent alone , which further documents the pharmacologic synergism of these agents . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Synergism of tissue type plasminogen activator ( t PA ) and single chain urokinase type plasminogen activator ( scu PA ) on clot lysis in vitro and a mechanism for this effect . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human urokinase type plasminogen activator stimulates chemotaxis of human neutrophils . ^^^ Human purified urokinase type plasminogen activator ( u PA ) stimulates chemoattractant activity for human neutrophils using modified Boyden chambers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recombinant tissue type plasminogen activator ( rt PA ) and single chain urokinase type plasminogen activator ( scu PA ) are thrombolytic agents , characterized by a high but not absolute degree of fibrin specificity that is mediated through different molecular mechanisms . ^^^ Research is being pursued in this area along the following lines : 1 ) tissue type plasminogen activator ( t PA ) and single chain urokinase type plasminogen activator in molar ratios of 4 : 1 to 1 : 4 do not act synergistically on thrombolysis in a plasma environment in vitro , but display significant synergism in animal models of thrombosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Involvement of urokinase type plasminogen activator in acantholysis induced by pemphigus IgG . ^^^ Urokinase type plasminogen activator , a proteolytic enzyme , has been implicated in the development of acantholysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Selective fibrinolytic activity of recombinant non glycosylated human pro urokinase ( single chain urokinase type plasminogen activator ) from bacteria . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We compared three methods of lung metastasis detection : histology , detection of tumor cells in the cultures of lung minces , and the measurement of the levels of human urokinase type plasminogen activator directly in the lysates of lungs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The transcription of genes which are positively regulated by glucocorticoids ( e . g . , tissue type plasminogen activator and c myc in mammary cells , c fos in macrophages ) and that of genes which are negatively regulated by these agents ( e . g . , urokinase type plasminogen activator in all three cell types , TNF a and IL 1 in macrophages ) was explored . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Sensitive and specific enzyme linked immunosorbent assay for urokinase type plasminogen activator and its application to plasma from patients with breast cancer . ^^^ An enzyme linked immunosorbent assay ( ELISA ) was developed for the measurement of human urokinase type plasminogen activator ( u PA ) in plasma and serum . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human plasma contains two physiologic activators of the fibrinolytic system : prourokinase , also called single chain urokinase type plasminogen activator ( scu PA ) , and tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ultrastructural localization of plasma membrane associated urokinase type plasminogen activator at focal contacts . ^^^ We have recently shown that urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor type 1 are both found extracellularly beneath cultured human skin fibroblasts and HT 1080 sarcoma cells , but in distinct localizations . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Synergistic effect on thrombolysis of sequential infusion of tissue type plasminogen activator ( t PA ) single chain urokinase type plasminogen activator ( scu PA ) and urokinase in the rabbit jugular vein thrombosis model . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator is a protease existing in two forms known as tissue type and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical localization of urokinase type plasminogen activator in Sertoli cells and tissue type plasminogen activator in spermatogenic cells in the rat seminiferous epithelium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Construction and expression of hybrid plasminogen activators prepared from tissue type plasminogen activator and urokinase type plasminogen activator genes . ^^^ Recent data from several studies have suggested that the non protease domains in tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) determine their biological specificities , including binding to fibrin clots and survival in the circulatory system ( Van Zonneveld , A . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator ( scu PA ) , was given to 20 patients with acute myocardial infarction first alone ( group 1 ; n = 9 ) and then in combination with an initial bolus injection of 200 , 000 units of urokinase ( group 2 ; n = 11 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation is mediated by plasminogen activators which are classified as either tissue type plasminogen activator ( t PA ) or urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator levels , although elevated in three patients , were disassociated from increased t PA levels and concomitant systemic fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator ( scu PA , pro urokinase ) is more fibrin specific than urokinase but has only reached the stage of early clinical investigation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator is a serine protease which exists in two forms , known as tissue type plasminogen activator and urokinase type plasminogen activator . ^^^ Here , we show that urokinase type plasminogen activator activity in primary breast carcinomas correlates with both size of tumor and number of axillary nodes with metastases . ^^^ Patients with primary carcinomas containing high levels of urokinase type plasminogen activator activity had a significantly shorter disease free interval than patients with low levels of activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Correlation between urokinase type plasminogen activator production and the metastasizing ability of two murine mammary adenocarcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased plasma levels of urokinase type plasminogen activator with endometrial and cervical cancer . ^^^ Plasma concentrations of urokinase type plasminogen activator ( competitive radioimmunoassay ) , tissue type plasminogen activator ( sandwich enzyme linked immunosorbent assay ) , and plasminogen activator inhibitor ( functional assay ) were measured in 17 women with endometrial cancer and 52 women with cervical carcinoma . ^^^ Significantly increased plasma urokinase type plasminogen activator antigen levels were found ( 11 . 3 + / 4 . 7 ng / mL ) in cervical cancer patients when compared with an age matched control group ( 7 . 4 + / 0 . 6 ng / mL ) . ^^^ Women with endometrial cancer ( 9 . 9 + / 2 . 0 ng / mL ) showed a similar pattern of plasma urokinase type plasminogen activator antigen levels . ^^^ Patients with advanced cervical cancer ( International Federation of Gynecology and Obstetrics stages 3 and 4 ) revealed higher plasma urokinase type plasminogen activator antigen levels than those with stages 1 and 2 disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Blood was collected at least 3 months after the last acute episode , and PAI 1 antigen and activity , as well as tissue type plasminogen activator ( t PA ) antigen , urokinase type plasminogen activator ( u PA ) antigen , and fibrinolytic activity were measured in these samples . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Kinetics of inhibition of tissue type and urokinase type plasminogen activator by plasminogen activator inhibitor type 1 and type 2 . ^^^ Highly purified plasminogen activator inhibitors of type 1 ( PAI 1 ) and type 2 ( PAI 2 ) , low Mr form , were compared with respect to their kinetics of inhibition of tissue type ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Modulation of urokinase type plasminogen activator messenger RNA levels in human synovial fibroblasts by interleukin 1 , retinoic acid , and a glucocorticoid . ^^^ Previous studies have shown that mononuclear cell conditioned medium ( MCCM ) , interleukin 1 ( IL 1 ) , and all trans retinoic acid rapidly stimulate , while glucocorticoids lower , the urokinase type plasminogen activator ( u PA ) activity of human synovial fibroblast like cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recombinant single chain urokinase type plasminogen activator ( rscu PA ) induces thrombolysis and systemic fibrinolysis in a canine model of coronary artery thrombosis . ^^^ The thrombolytic efficacy of recombinant single chain urokinase type plasminogen activator ( rscu PA ) was studied in an open chest canine model of coronary artery thrombosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recombinant DNA technology has allowed large scale production of the physiological , fibrin specific , plasminogen activators tissue type plasminogen activator ( t PA ) and single chain urokinase type plasminogen activator ( scu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparison of intracoronary single chain urokinase type plasminogen activator , pro urokinase ( GE 0943 ) , and intracoronary urokinase in patients with acute myocardial infarction . ^^^ Coronary recanalization rates and changes in the coagulation and fibrinolysis system were evaluated in a randomized fashion in patients with acute myocardial infarction after intracoronary administration of single chain urokinase type plasminogen activator ( pro urokinase : GE 0943 ) or urokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator ( scu PA ) activates plasminogen directly but with a catalytic efficiency which is about 20 times lower than that of urokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator in colorectal carcinomas and adenomatous polyps : quantitative expression of active and proenzyme . ^^^ Total urokinase type plasminogen activator ( u PA ) content ( proenzyme plus active enzyme ) was significantly higher in 20 colorectal carcinomas and in 27 adenomatous polyps than in metaplastic polyps and autologous normal mucosa . u PA content was also markedly increased in adenomatous polyps and autologous colonic mucosa removed from familial polyposis coli patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Isolation and interrelationships of the multiple molecular tissue type and urokinase type plasminogen activator forms produced by cultured human umbilical vein endothelial cells . ^^^ Later subcultures ( greater than 4th passage ) produce increasing amounts of t PA antigen , consisting of a major Mr 110 , 000 and a minor Mr 68 , 000 form as well as increasing amounts of urokinase type plasminogen activator ( u PA ) antigen , consisting of a minor Mr 95 , 000 and major Mr 54 , 000 form . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The granulosa cells of 49 follicles from 20 patients undergoing in vitro fertilization were obtained by laparoscopy and tested for the content of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) and inhibitor of plasminogen activator ( PAI ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Modulation of urokinase type plasminogen activator and plasminogen activator inhibitor 2 expression by U 937 mononuclear phagocytes . ^^^ Mononuclear phagocytes are known to produce both urokinase type plasminogen activator ( u PA ) and a specific PA inhibitor , PAI 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Purification and properties of a single chain urokinase type plasminogen activator form produced by subcultured human umbilical vein endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Primary cultures of renal cell carcinomas and of the corresponding normal adjacent kidney tissue from 6 patients were analyzed for the effects of exogenously added urokinase type plasminogen activator on cell proliferation as compared to the effects of tissue type plasminogen activator , plasmin and dihydrocortisone . ^^^ It is concluded that urokinase type plasminogen activator itself exhibits a mitogenic effect also on primary cultures of renal cell carcinomas . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PAI 1 inhibits both tissue type plasminogen activator and urokinase type plasminogen activator and may therefore be implicated in the control of various physiological processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human endometrial adenocarcinoma HEC 1 A and HEC 1 B cells produce and secrete the urokinase type plasminogen activator and trace amounts of the tissue type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The only thrombolytic agents with a relative fibrin selectivity presently available for clinical purposes are tissue type plasminogen activator and single chain urokinase type plasminogen activator . ^^^ Also chimaeric molecules combining fragments of tissue type plasminogen activator and single chain urokinase type plasminogen activator have already been constructed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cells of the human myelomonocytic line RC 2A synthesize tissue factor like procoagulant and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Three different commercially available plasminogen preparations ( Immuno , Kabi , and Behring plasminogens ) were examined regarding purity and reactivity to different activators ( high molecular weight [ HMW ] or low molecular weight [ LMW ] two chain urokinase type plasminogen activator [ tcu PA ] , single chain urokinase type plasminogen activator [ scu PA ] , tissue type plasminogen activator [ t PA ] , and streptokinase [ SK ] ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enhanced myocardial salvage by combined treatment with recombinant single chain urokinase type plasminogen activator and recombinant human superoxide dismutase in a canine coronary thrombosis model . ^^^ The myocardial salvage by the fibrin specific recombinant single chain urokinase type plasminogen activator ( r scu PA ) alone and in combination with the oxygen radical scavenger human superoxide dismutase of recombinant origin ( r HSOD ) was investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Gel electrophoretic analysis of the protein products showed that the 23S fraction of poly ( A ) + RNA from human kidney contains mRNA for single chain urokinase type plasminogen activator with apparent molecular weight of approximately 50 kDa . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Multiple nuclear factors interact with promoter sequences of the urokinase type plasminogen activator gene . ^^^ To characterize proteins that bind to the cyclic AMP inducible promoter of the urokinase type plasminogen activator gene , we performed a DNAase 1 footprinting analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Together with t PA , human microvascular cells release urokinase type plasminogen activator ( u PA ) antigen and endothelial cell type PA inhibitor , PA inhibitor 1 , which were both demonstrated by specific immunoprecipitation from radiolabeled endothelial cell conditioned medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the 125I fibrin plate assay one of the antibodies neutralized the effect of the inhibitor on urokinase type plasminogen activator . ^^^ Two of the antibodies bound complexes between urokinase type plasminogen activator and inhibitor while the remaining two antibodies did not . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effect of the cyanogen bromide 2 fragment of fibrinogen on plasminogen activation by single chain urokinase type plasminogen activator . ^^^ Activation of Glu plasminogen by single chain urokinase type plasminogen activator ( sc uPA ) , isolated from human urine , was studied in a purified system in the absence and presence of the cyanogen bromide fibrinogen fragment , FCB 2 , and compared to plasminogen activation by two chain high Mr urokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Pharmacokinetics of single chain forms of urokinase type plasminogen activator . ^^^ The pharmacokinetics of single chain urokinase type plasminogen activator ( scu PA ) were studied in rabbits and squirrel monkeys using three of its molecular forms , Mr 54 , 000 isolated from human urine ( urinary scu PA ) , Mr 54 , 000 isolated from conditioned media of cultured human lung adenocarcinoma cells ( CALU 3 ) ( cellular scu PA ) and its Mr 32 , 000 proteolytic derivative lacking the NH 2 terminal 143 amino acids ( scu PA 32k ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To study the relationship between PA expression and the development of colorectal cancer , we determined urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) activity in normal mucosa ( n = 80 ) , adenomatous polyps ( n = 76 ) , and adenocarcinomas ( n = 71 ) of the colon . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparative kinetic analysis of the activation of human plasminogen by natural and recombinant single chain urokinase type plasminogen activator . ^^^ Single chain urokinase type plasminogen activator ( scu PA ) may be obtained from conditioned cell culture media ( natural scu PA ) or by expression of the cDNA encoding human scu PA in Escherichia coli ( recombinant scu PA ) . ^^^ Subsequent conversion of scu PA to urokinase ( two chain urokinase type plasminogen activator , tcu PA ) by generated plasmin ( reaction 2 ) occurs with a comparable affinity ( Km about 5 microM ) for natural and recombinant scu PA and with a k 2 of 0 . 23 s 1 for natural and 1 . 2 s 1 for recombinant scu PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Suppression of urokinase type plasminogen activator mRNA levels in human fibrosarcoma cells and synovial fibroblasts by anti inflammatory glucocorticoids . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Differential reactivity of Glu Gly Arg CH2Cl , a synthetic urokinase inhibitor , with single chain and two chain forms of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proenzyme to urokinase type plasminogen activator in the mouse in vivo . ^^^ We have investigated whether urokinase type plasminogen activator ( u PA ) is present in the mouse in vivo as the proenzyme or as the active enzyme . u PA in extracts of various murine tissues was of a one polypeptide chain form with an electrophoretic mobility indistinguishable from purified proenzyme ( pro u PA ) , as demonstrated by SDS polyacrylamide gel electrophoresis under reducing conditions followed by immunoblotting . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using antibodies specific for tissue type or urokinase type plasminogen activator , we have found that granulosa cells produce exclusively the tissue type enzyme . ^^^ However , in cultures of whole follicles isolated from the ovary , there is primarily synthesis of urokinase type plasminogen activator . ^^^ Examination of other isolated ovarian cell types has demonstrated that thecal cells secrete the urokinase type plasminogen activator and that the production of this enzyme is also regulated by gonadotropins and temporally correlated with ovulation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Correlation of active urokinase type plasminogen activator with urokinase antigen revealed that in tumor tissue the enzyme was present to more than 70 % in its active form whereas in tumor adjacent tissue and normal renal tissue only half of the enzyme appeared to be active . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Adherent monolayer cultures of human blood monocytes , peritoneal macrophages , bone marrow macrophages , and colonic mucosa macrophages were examined for their ability to produce and secrete minactivin , a specific inactivator of urokinase type plasminogen activator . ^^^ The minactivin from each of these populations could be shown to preferentially inhibit urokinase type plasminogen activator and not trypsin , plasmin , or `` tissue ' ' type plasminogen activator ( HPA 66 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
All plasminogen activator activity was of the tissue type ( t PA ) as judged from the following criteria : all enzyme activity was inhibited by a monoclonal antibody against human t PA , while there was no measurable inhibition by a monoclonal antibody against human urokinase type plasminogen activator ( u PA ) ; as determined by zymography the extracts contained one type of plasminogen activator which had an electrophoretic mobility identical to that of purified t PA ; this plasminogen activator was removed by passage through a Sepharose column coupled with a monoclonal antibody against t PA , but not by passage through a column coupled with a monoclonal antibody against u PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator in alveolar macrophages and bronchoalveolar lavage fluid from normal and smoke exposed hamsters and humans . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunochemical characterisation of these plasminogen activators showed that they included both tissue type and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunocytochemical localization of urokinase type plasminogen activator in Lewis lung carcinoma . ^^^ The invasively growing and metastasizing Lewis lung carcinoma consistently contained urokinase type plasminogen activator ( u PA ) enzyme activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Confluent cultures of bovine aortic endothelial cells ( BEC ) were found to secrete both tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Active bands of free and complexed tissue type plasminogen activator ( t PA ) or urokinase type plasminogen activator ( u PA ) were identified by the incorporation of specific antibodies against , respectively , t PA or u PA in the indicator gel . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Isolation and characterization of a urokinase type plasminogen activator ( Mr = 54 , 000 ) from cultured human endothelial cells indistinguishable from urinary urokinase . ^^^ A urokinase type plasminogen activator secreted by subcultured normal human umbilical vein endothelial cells was purified and compared to urinary urokinase ( Mr = 54 , 000 ) . ^^^ These results indicate that the Mr = 54 , 000 urokinase type plasminogen activator from cultured normal human endothelial cells is similar to , or identical with , Mr = 54 , 000 urinary urokinase . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Protection of single chain urokinase type plasminogen activator ( scu PA ) in aprotinin treated cardiac surgical patients undergoing cardiopulmonary bypass . ^^^ Here we report on the effects of aprotinin on urokinase type plasminogen activator , especially on the protection of the zymogen single chain urokinase type plasminogen activator ( scu PA ) . scu PA occurs cell associated as well as free in the circulation ( concentration 50 pM , half life 5 min ) , and is potentially activated by kallikrein and plasmin , both potent targets for aprotinin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of c ets 1 , collagenase 1 , and urokinase type plasminogen activator genes in lung carcinomas . ^^^ Urokinase type plasminogen activator mRNAs were expressed in 45 / 54 lung carcinomas in stromal and / or cancer cells . ^^^ These results demonstrate that the transcription factor c ets 1 , collagenase 1 , and urokinase type plasminogen activator are involved in lung cancer invasion and suggest that c ets 1 protein might transactivate collagenase 1 gene during tumor invasion . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Detection of mRNAs for urokinase type plasminogen activator , its receptor , and type 1 inhibitor in giant cell tumors of bone with in situ hybridization . ^^^ We therefore evaluated the mRNA levels for urokinase type plasminogen activator ( u PA ) , urokinase type plasminogen activator receptor ( u PAR ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) by using Northern blot analysis and in situ hybridization in four cases of GCT and spindle shaped mononuclear cells at the 35th passage from a GCT . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Epidermal growth factor binding protein activates soluble and receptor bound single chain urokinase type plasminogen activator . ^^^ We analyzed the reaction of EGF BP with urokinase type plasminogen activator ( u PA ) , a serine proteinase that promotes pericellular proteolysis and cellular migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both neoantigenic epitopes were also exposed after complex formation between PAI 1 and either urokinase type plasminogen activator , plasmin or thrombin as well as after cleavage of the reactive site loop of non inhibitory substrate type PAI 1 variants . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Basic fibroblast growth factor stimulated cell proliferation , cell migration , tubulogenesis and the expression of urokinase type plasminogen activator ( u PA ) in bovine aortic endothelial ( BAE ) cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased urokinase type plasminogen activator ( u PA ) levels in graft liver perfusate and decreased single chain u PA activation with higher levels of aprotinin . ^^^ In the present investigation we focused on the reperfusion of the graft liver in order to study the hepatic influence in the regulation of urokinase type plasminogen activator ( u PA levels ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Suramin , an anticancer and angiosuppressive agent , inhibits endothelial cell binding of basic fibroblast growth factor , migration , proliferation , and induction of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It has considerable homology with plasminogen and has been shown to upregulate urokinase type plasminogen activator ( u PA ) in endothelial cells as well as u PA and its receptor in kidney epithelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The apparent kinetic constants of plasminogen activation by receptor bound urokinase type plasminogen activator ( u PA ) compared with solution phase u PA [ Michaelis constant ( Km ) = 0 . 80 + / 0 . 54 vs . 3 . 15 + / 0 . 78 microM , P < 0 . 0001 ; catalytic constant ( kcat ) = 0 . 39 + / 0 . 17 vs . 0 . 50 + / 0 . 29 s 1 , not significant ; kcat / Km = 0 . 57 + / 0 . 35 vs . 0 . 16 + / 0 . 11 microM 1 . s 1 , P < 0 . 05 , respectively ] showed a higher efficiency of plasminogen activation at the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
CD 59 has no structural similarity to other complement proteins , but is an example of a plasma protein domain type found also in murine Ly 6 proteins and the urokinase type plasminogen activator receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The activity of secreted urokinase type plasminogen activator and active interstitial collagenase , but not gelatinase , was inhibited by NSC 639366 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cellular regulatory sequences responsive to the c Ets 1 proteins include a urokinase type plasminogen activator ( u PA ) gene enhancer , the stromelysin 1 and the collagenase 1 gene promoters . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Pharmacokinetics and pharmacodynamics of saruplase , an unglycosylated single chain urokinase type plasminogen activator , in patients with acute myocardial infarction . ^^^ We examined in patients with acute myocardial infarction ( AMI ) the pharmacokinetics of saruplase , an unglycosylated , single chain , urokinase type plasminogen activator ( rscu PA ) by measuring urokinase type plasminogen activator ( u PA ) antigen and total u PA activity , its conversion to active two chain urokinase type plasminogen activator ( tcu PA ) and evaluated its effect on haemostatic parameters . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen ( PG ) , urokinase type plasminogen activator ( u PA ) and tissue type PA ( t PA ) are the main molecules involved in fibrinolysis and in many other physiological and pathological processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Coordinated expression of the vitronectin receptor and the urokinase type plasminogen activator receptor in metastatic melanoma cells . ^^^ In the present study , the expression and function of alpha 5 beta 3 were further investigated with emphasis on the functional relationship between alpha 5 beta 3 and the urokinase type plasminogen activator system of proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Angiogenin up regulated both mRNA level and activity of urokinase type plasminogen activator , a key mediator of angiogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although urokinase type plasminogen activator ( u Pa ) activity was not detected at premalignant stages , it began to appear early in tumorigenesis and became more prominent in later stages of a majority of the tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When compared to parental cells , bFGF B 9 cells showed down regulation of tyrosine kinase fibroblast growth factor receptors along with up regulation of urokinase type plasminogen activator expression which was abolished by incubation of the cell cultures with neutralizing anti bFGF antibody . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have followed the synthesis and secretion of urokinase type plasminogen activator ( u PA ) and its inhibitor , PAI 1 , and matrix metalloproteinases ( MMPs ) and tissue inhibitor of metalloproteinases ( TIMP 1 ) during differentiation of a human osteoblastic cell line , HOS TE 85 , and the effect of TNF alpha on this process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At two to six hours stimulated expression of the genes encoding plasminogen activator inhibitor ( PAI 1 ) and platelet derived growth factor ( PDGF ) A chain was detected , but constitutive expression of urokinase type plasminogen activator ( u PA ) was not altered . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To evaluate the role of mesothelial cells ( MC ) in the high peritoneal fibrin turnover we investigated the expression of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) , and tissue factor in cultured human peritoneal MC under basal conditions and after exposure to tumour necrosis factor alpha ( TNF alpha ) , interleukin 1 alpha ( IL 1 alpha ) , or bacterial lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This was due to a combination of inefficient mRNA translation and mRNA instability , caused by elements from the untranslated portions of tissue type and urokinase type plasminogen activator cDNA which were included in the expression vector . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen and urokinase type plasminogen activator ( u PA ) constitute one of the most extensively studied proteolytic systems believed to participate in these processes . u PA cleaves plasminogen to plasmin , which in turn degrades surrounding extracellular matrix and allows tumor cells to migrate to other areas . ^^^ Cross linking experiments showed that the approximately 55 kDa band only , and not the approximately 41 kDa band , was able to bind either single chain urokinase type plasminogen activator ( scu PA ) or the amino terminal fragment of urokinase ( ATF ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase activity detected in the conditioned media was depressed by Pefabloc , suggesting that the urokinase type plasminogen activator was involved in the proteolysis of IGFBP 3 . ^^^ We conclude from these results 1 ) that IGF 2 is involved in the autocrine control of PC 3 cell proliferation via the type 1 IGF receptor ; and 2 ) that this proliferation is directly dependent on IGF 2 bioavailability that itself is modulated by the limited IGFBP 3 proteolysis induced , at least in part , by urokinase type plasminogen activator and plasmin . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This vector carries a cDNA fragment encoding the protease domain of human urokinase type plasminogen activator ( u PA ) as a reporter gene under the SV 40 early promoter . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Message levels of urokinase type plasminogen activator ( u PA ) and its inhibitor ( PAI 1 ) and heat shock protein ( HSP ) 70 are markedly raised 4 8 hours after wounding . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These findings suggest the possibility that the cerebral microvasculature may be a specialized region of the vascular system in which urokinase type plasminogen activator , not tissue type plasminogen activator , is the key catalyst of fibrin lysis when the brain responds to thrombotic events and that alpha thrombin may regulate repair of the cerebral microvascular system . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prognostic role of urokinase type plasminogen activator in human gliomas . ^^^ Urokinase type plasminogen activator ( u PA ) is a 54 kd enzyme shown to participate in tissue degradation under certain normal and pathological conditions , including cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator has been administered by other investigators to patients with small cell carcinoma of the lung ( SCCL ) in an attempt to induce lysis of fibrin that is known to exist in the connective tissue stroma of this tumour type and that may support tumour growth . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A conserved TATA less proximal promoter drives basal transcription from the urokinase type plasminogen activator receptor gene . ^^^ The urokinase type plasminogen activator receptor ( uPAR ) focuses at the cell surface the activation of pro uPA and , hence , the formation of plasmin , thus enhancing directional extracellular proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombolysis with recombinant unglycosylated single chain urokinase type plasminogen activator ( saruplase ) in acute myocardial infarction : influence of heparin on early patency rate ( LIMITS study ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen / plasmin or fibrinolytic system with its physiological triggers , tissue type plasminogen activator , and urokinase type plasminogen activator has been presumed to participate in normal and pathological processes of the vessel wall such as blood clot dissolution ( thrombolysis ) , hemostasis , aneurysm formation , neovascularization , restenosis , and atherosclerosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
On the other hand , the levels of 90K were positively correlated with those of cytosolic estrogen receptor , progesterone receptor , urokinase type plasminogen activator , its inhibitor PAI 1 , cathepsin D and PS 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Further , although it is well known that scuPA binds to a specific extracellular urokinase type plasminogen activator receptor , the effect of this interaction on the enzymatic activity of scuPA has not been described . ^^^ In the present paper we report that the binding of scuPA to cellular an to recombinant soluble urokinase type plasminogen activator receptors ( suPAR ) increases its catalytic activity as measured by the cleavage of a urokinase specific chromogenic substrate . suPAR increased the Vmax of scuPA 5 fold with little change in its Km . suPAR also stimulated the plasminogen activator activity of scuPA by decreasing its Km for Glu plasminogen from 1 . 15 microM to 0 . 022 microM and by increasing the kcat of this reaction from 0 . 0015 to 0 . 022 s 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
All specimens exhibited specific areas of inflammatory infiltrates with macrophage like cells expressing urokinase type plasminogen activator ( u PA ) and tissue type PA ( t PA ) mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase type plasminogen activator is not essential for mouse development or fertility . ^^^ The urokinase type plasminogen activator receptor ( uPAR ) gene was disrupted in mice in order to explore the role of cell surface associated plasminogen activation in development and hemostasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To elucidate the pathophysiology of idiopathic pulmonary fibrosis ( IPF ) , we examined procoagulant ( tissue factor : TF ) , fibrinolytic ( tissue type plasminogen activator : t PA and urokinase type plasminogen activator : u PA ) and antifibrinolytic ( plasminogen activator inhibitor 1 : PAI 1 and PAI 2 ) activities in bronchoalveolar lavage ( BAL ) supernatant fluids and BAL cell lysates obtained from IPF patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator plays a central role in tissue remodeling by controlling the synthesis of the extracellular matrix degrading plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Indomethacin treatment of the M phi also prevents M . avium dependent accumulation of mRNA encoding plasminogen activator inhibitor type 2 ( PAI 2 ) , an inhibitor of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator mRNA was found in basal keratinocytes in seven of nine samples . ^^^ Collagenase , stromelysin 1 , and urokinase type plasminogen activator were not expressed in normal appearing skin of patients with dermatitis herpetiformis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The ability of U 937 monocyte like cells and KATO 3 cells ( a human gastric carcinoma line ) to potentiate activation of plasminogen by single chain urokinase type plasminogen activator ( scu PA ) , as mediated by the cell receptor for urokinase ( u PAR ) , was compared . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen bound to the surface of B . burgdorferi could be converted into plasmin by a human urokinase type plasminogen activator . 125I labeled plasminogen ligand blots of borrelial membrane proteins demonstrated two prominent binding proteins at approximately 70 and approximately 30 kDa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Like tissue type plasminogen activator and two chain urokinase type plasminogen activator , pro urokinase has a very short half life in circulation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using in situ hybridization to analyze the coexpression of laminin 5 and components of the plasminogen activation system , we found that the histological distribution of laminin 5 positive budding cancer cells at the invasion front in colon adenocarcinomas was identical to that of the receptor for urokinase type plasminogen activator . ^^^ The colocalization of laminin 5 and urokinase type plasminogen activator receptor in a subset of cancer cells in colon cancer also suggests that a controlled up regulation of a number of gene products is a characteristic of budding colon cancer cells , and that these gene products serve functions crucial for the invasive phenotype of these cancer cells . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Low M ( r ) urokinase type plasminogen activator as a reporter protein . ^^^ A reporter plasmid coding for the low molecular weight ( low M ( r ) ) form of single chain urokinase type plasminogen activator ( low M ( r ) u PA ; Leu 144 Leu411 ) has been constructed and used to analyze promoter activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Nude mice have been subcutaneously inoculated with human tumorigenic fibrosarcoma cells ( HT 1080 ) producing urokinase type plasminogen activator ( u PA ) or with human tumorigenic melanoma cells ( G 361 ) producing tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of the endothelial cell urokinase type plasminogen activator receptor . ^^^ Binding of urokinase type plasminogen activator ( u PA ) to specific receptors ( u PAR ) on the surface of endothelial cells contributes to the regulation of plasmin dependent processes such as fibrinolysis and angiogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zymographic and immunological studies revealed that primarily tissue type plasminogen activator and to a lesser extent urokinase type plasminogen activator were present in fluids of pemphigus vulgaris ( type Neumann ) skin blisters . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator : a paracrine factor regulating the bioavailability of IGFs in PA 3 cell induced osteoblastic metastases . ^^^ Sequence analysis identified the amino acid sequence of the a chain ( 20 25 kDa band ) and of the b chain ( 33 35 kDa band ) of rat urokinase type plasminogen activator molecule . ^^^ Northern blot analysis documented the expression of the urokinase type plasminogen activator gene in the mRNA extracted from PA 3 cells . ^^^ Therefore , we conclude that urokinase type plasminogen activator stimulates osteoblasts via an IGF 1 dependent mechanism . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA , 0 . 3 and 1 mg / kg ) , single chain urokinase type plasminogen activator ( scu PA , 5 and 15 mg / kg ) and a novel thrombolytic agent , staphylokinase ( SAK , 0 . 3 and 1 mg / kg ) were intravenously infused for 30 min after arterial occlusion by a photochemically induced reaction between rose bengal and light ( 540 nm ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biological effects of disruption of the tissue type plasminogen activator , urokinase type plasminogen activator , and plasminogen activator inhibitor 1 genes in mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation by two chain urokinase type plasminogen activator ( urokinase ) , two chain tissue type plasminogen activator ( tc tPA ) or trypsin , but not by single chain tPA ( sc tPA ) is increased 5 to 10 fold by both substrates , as determined by electrophoretic and spectrophotometric kinetic analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since the plasminogen activator [ PA / plasminogen activator inhibitor ( PAI ) system is believed to be involved in a breakdown of articular cartilage in osteoarthritis ( OA ) , we studied the modulation of single components of the fibrinolytic system ( urokinase type plasminogen activator , u PA ; plasminogen activator inhibitor 1 , PAI 1 ; the surface receptor for u PA , u PAR ) in human chondrocytes in the presence of piroxicam . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A remarkable finding , considering the well established linkage between plasmin and the proteolytic activation of plasminogen activators , was that the level of active urokinase type plasminogen activator in urine was unaffected in Plg / mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated immunohistochemically the localization of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , plasmin inhibitor ( PI ) , and transforming growth factor beta ( TGA beta ) in tissue sections to examine the relationships between their localization and local invasiveness , tumor size and cervical lymph node metastasis of head and neck squamous cell carcinomas ( SCCs ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the influence of changes in liver blood flow on the pharmacokinetics and pharmacodynamics of single chain unglycosylated urokinase type plasminogen activator . ^^^ Indocyanine green concentrations , total urokinase type plasminogen activator ( u PA ) antigen , two chain u PA activity , fibrinogen , total degradation products , alpha 2 antiplasmin , and factor 12 dependent fibrinolytic activity were measured . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effect of cyclic AMP on urokinase type plasminogen activator receptor and fibrinolytic factors in a human osteoblast like cell line . ^^^ Dibutyryl cAMP ( dbcAMP ) or forskolin increased the level of urokinase type plasminogen activator ( u PA ) mRNA and enhanced the secretion of u PA antigen into the conditioned medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator or pro urokinase ( pro UK ) has been reported to have a significant intrinsic amidolytic and plasminogen activator activity , estimated to be about 0 . 2 0 . 6 % that of two chain urokinase ( UK ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The regulatory role of fibrin in plasminogen activation involving its direct interaction with tissue type plasminogen activator and indirect interaction with urokinase type plasminogen activator is demonstrated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Receptors were labeled using fluorescein , rhodamine , or AMCA conjugated F ( ab ' ) 2 fragments of anti Fc gamma RIIIB ( CD 16 ) , anti CR 3 ( CD11b / CD18 ) , and anti uPAR ( urokinase type plasminogen activator receptor ) antibodies , intact phycoerythrin labeled interleukin 8 , and fluorescein or rhodamine labeled Con A ( concanavalin A ) , Boc PLPLP ( tert butyl oxycarbonyl Phe ( D ) Leu Phe ( D ) Leu Phe OH ) , and N formyl Nle Leu Phe Nle Tyr Lys . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Localization of urokinase type plasminogen activator , plasminogen activator inhibitor 1 , 2 and plasminogen in colon cancer . ^^^ We examined the localization of urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitors ( PAI 1 and PAI 2 ) and plasminogen ( plg ) in 26 cases of colon cancer by immunohistochemical staining . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two immunologically distinct plasminogen activators have been identified : tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolysis and invasiveness of brain tumors : role of urokinase type plasminogen activator receptor . ^^^ The cellular receptor for urokinase type plasminogen activator ( uPAR ) in glioblastoma cell lines has been identified and found to be similar to the uPAR expressed by other tumor cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine whether vascular smooth muscle cells within atheroma express the receptor for urokinase type plasminogen activator ( uPA R ) , we characterized atheroma in cholesterol fed New Zealand White rabbits and human subjects by immunostaining . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombin induced a rapid ( < 5 minute ) , time ( 0 to 30 minutes ) and dose ( 0 . 1 to 8 U / mL ) dependent decrease in the specific binding of 125I labeled two chain urokinase type plasminogen activator ( tcu PA ) or diisopropylfluoro phosphate tcu PA to urokinase type plasminogen activator receptor ( u PAR ) in cultured ECs from various sources ( range , 21 % to 50 % ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Receptors have been described on a wide range of cell lines for many such proteinases [ urokinase type plasminogen activator ( u PA ) , plasminogen / plasmin , collagenase ] , which subsequently activate each other on the solid phase of the cell surface , leading to cartilage destruction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression and characterization of a chimeric bispecific antibody against fibrin and against urokinase type plasminogen activator . ^^^ We have produced a chimeric bispecific antibody that has dual specificity of human fibrin and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Five products of human breast carcinoma cells , including membrane associated phospholipase A 2 ( M PLA 2 ) , polymorphonuclear leukocyte elastase ( PMN E ) , tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , and endothelin 1 ( ET 1 ) , have been implicated in the processes of tumor cell invasion and metastasis in human breast carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In a placebo controlled , blinded crossover study , eight volunteers were challenged twice with an intravenous bolus injection of endotoxin ( 40 EU / kg of body weight ) and concurrently received either rBPI 23 ( 1 mg / kg ) or placebo ( human serum albumin , 0 . 2 mg / kg ) . rBPI 23 treatment significantly lowered the endotoxin induced fibrinolytic response , ie , reduced the release of tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor antigen , and complex formation of plasmin alpha 2 antiplasmin ( P = . 0078 for each ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activated gelatinase B ( MMP 9 ) and urokinase type plasminogen activator in synovial fluids of patients with arthritis . ^^^ In paired ( series 1 ) and unpaired SF ( series 2 ) we examined the occurrence of gelatinase B , prostromelysin 1 , and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Exogenously provided urokinase type plasminogen activator converted B . burgdorferi surface bound plasminogen to enzymatically active plasmin as demonstrated by the breakdown of the chromogenic plasmin substrate S 2251 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The open conformation of rPlg variants leads to an increased rate of activation by urokinase type plasminogen activator and streptokinase and increased binding to a fibrin clot . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of the receptor for urokinase type plasminogen activator in normal and neoplastic blood cells and hematopoietic tissue . ^^^ Expression of the receptor for the urokinase type plasminogen activator ( uPAR ) has been studied by flow cytometry and immunohistology in normal blood and bone marrow cells , in vitro activated lymphoid cells , and tissue samples from reactive lymph nodes ( n = 6 ) , thymus ( n = 2 ) and malignant lymphomas ( n = 82 ) , or leukemias ( n = 32 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Backbone dynamics of a two domain protein : 15N relaxation studies of the amino terminal fragment of urokinase type plasminogen activator . ^^^ The amino terminal fragment ( ATF ) of urokinase type plasminogen activator ( u PA ) is a two domain protein which consists of a kringle and a growth factor domain ( GFD ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
LS 174T exhibited high cell associated urokinase type plasminogen activator ( u PA ) and high secreted u PA and tissue plasminogen activator ( t PA ) levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We compared the thrombolytic properties of recombinant staphylokinase ( SAK ) with those of streptokinase ( SK ) , a tissue type plasminogen activator ( t PA ) and a urokinase type plasminogen activator ( u PA ) in the jugular vein thrombosis model in the rabbit in vivo and a circulating human plasma system in vitro . 50 % thrombolysis was observed at 360 min after intravenous infusion into rabbits of 150 micrograms / kg of SAK or 500 micrograms / kg of t PA , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These results suggest that fucoidan specifically binds to not only the heparin binding domain ( s ) of laminin but also site ( s ) other than the cell surface laminin receptor . 3 LL cells secreted a 50 kDa form of urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The conversion of normal appearing colonic mucosa to neoplastic tissue in these patients was associated with an increase in urokinase type plasminogen activator and plasminogen activator inhibitor type 1 , accompanied by a decreased level of tissue type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of urokinase type plasminogen activator ( u PA ) to its receptor ( u PA R ) is required for morphological and functional maturation during monocyte differentiation of the promyelocytic leukaemia line HL 60 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Chimeric plasminogen activators ( Figure 4 ) consisting of parts of t PA and the single chain urokinase type plasminogen activator ( scu PA ) did not significantly improve at the same time fibrin specificity and thrombolytic potency of the natural occurring molecules . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prourokinase is a single chain urokinase type plasminogen activator ( scu PA ) . ^^^ With the standard dosage regimen about 28 % of saruplase is converted into two chain urokinase type plasminogen activator ( tcu PA ) , which is rapidly inactivated by plasma inhibitors whereas saruplase is not . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to identify the individual contributions of the kringle ( K ) domains of human plasminogen ( Pg ) to the epsilon aminocaproic acid ( EACA ) induced stimulation of Pg activation by low molecular weight urokinase type plasminogen activator ( LMW uPA ) and inhibition of this same activation by Cl , we constructed the most conservative recombinant ( r ) Pg mutants possible that would greatly reduce the strength of the EACA binding site in the omega amino acid binding kringles , [ K1Pg ] ( [ D 139 > N ] r Pg ) , [ K4Pg ] ( [ D 413 > N ] r Pg ) , and [ K5Pg ] ( [ D 515 N ] r Pg ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human carcinomas of the oesophagus , stomach , colorectum , and their liver metastases were previously shown to have increased levels of the urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There was an inverse relation between cell density and mRNA expression for urokinase type plasminogen activator ( u PA ) and u PA receptor in both SCC 4 cells and keratinocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A total of 100 gastric adenocarcinomas , comprising 50 cases with lymph node metastasis and 50 cases without lymph node metastasis , were examined for immunohistochemical reactivity with the monoclonal antibody to urokinase type plasminogen activator ( u PA ) , Lex related 4C9 antigen , Jun , or to nucleobindin ( Nuc ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS AND RESULTS : The antithrombotic , thrombolytic , fibrinogenolytic , and pharmacokinetic properties of the following substances were determined in hamsters in the absence of conjunctive anticoagulant or antiplatelet therapy : recombinant tissue type plasminogen activator ( rTPA ) , recombinant single chain urokinase type plasminogen activator ( rscu PA ) , two chain urokinase type plasminogen activator ( UK ) , with a rTPA deletion mutant lacking amino acids 6 to 173 and a mutation N184E ( K2Pt ) , a rTPA / rscu PA chimeric plasminogen activator consisting of amino acids 1 to 3 and 87 to 274 of rTPA and amino acids 138 to 411 of rscu PA ( K1K2Pu ) , streptokinase ( SK ) , and recombinant staphylokinase ( STAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since vascular smooth muscle cell ( SMC ) migration may be an important event in atherosclerosis and in intimal thickening after vascular injury , we studied the cell surface expression of a receptor for urokinase type plasminogen activator ( u PAR ) in cultured human vascular SMC . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , intra individual comparisons were made of the concentrations of t PA , urokinase type plasminogen activator ( u PA ) , PAI 1 , and PAI 2 in GCF from the same sites before and after periodontal treatment in eight healthy male volunteers aged 35 46 yr . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma urokinase type plasminogen activator in patients with leukemias . ^^^ Plasma levels of urokinase type plasminogen activator ( u PA ) were measured with an enzyme linked immunosorbent assay in patients with leukemias . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Elevated urokinase type plasminogen activator plasma levels are associated with deterioration of liver function but not with hepatocellular carcinoma . ^^^ We measured urokinase type plasminogen activator ( u PA ) plasma levels in patients with various chronic liver diseases , including hepatocellular carcinoma ( HCC ) , also measuring these levels in healthy volunteers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In 20 tissue samples from human brain tumours the concentrations were measured of ( 1 ) total plasminogen activator activity , ( 2 ) tissue type plasminogen activator ( t PA ) activity , ( 3 ) urokinase type plasminogen activator ( u PA ) activity , and ( 4 ) t PA antigen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Rabbits were repeatedly immunized with a chimeric plasminogen activator composed of the kringle 2 domain of human tissue type plasminogen activator ( t PA ) attached to the serine protease domain of the human urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator and outcome in Dukes ' B colorectal cancer . ^^^ Urokinase type plasminogen activator ( u PA ) is a serine protease involved in cancer metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen variant displays no other significant differences from the wild type molecule with respect to activation by two chain urokinase type plasminogen activator , recognition by monoclonal antibodies , or ability to undergo conformational change . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Higher concentrations ( 7 20 micrograms / ml ) of the aminated derivative suppressed the adhesive ability of 3 LL cells to YIGSR . 3 LL cells secreted a 50 kDa form of urokinase type plasminogen activator ( u PA ) in the culture medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of the binding of urokinase type plasminogen activator ( u PA ) to plasminogen , to plasminogen activator inhibitor 1 and to the u PA receptor . ^^^ Binding parameters [ association rate ( kass ) and dissociation rate ( kdiss ) constants , and affinity constants ( KA = kass / kdiss ) ] for the interaction between urokinase type plasminogen activator ( u PA ) and its substrate plasminogen , its inhibitor plasminogen activator inhibitor 1 ( PAI 1 ) and its receptor ( u PAR ) , were determined by real time biospecific interaction analysis ( BIA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND / AIMS : Human colorectal carcinogenesis was previously found to be associated with an increased urokinase type plasminogen activator expression , both in antigen and activity , accompanied by simultaneously enhanced levels of plasminogen activator inhibitors type 1 and type 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The murine urokinase type plasminogen activator receptor gene . ^^^ The murine urokinase type plasminogen activator receptor ( uPAR ) gene has been isolated and its complete nucleotide sequence established . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Receptor binding and degradation of urokinase type plasminogen activator by human mesangial cells . ^^^ The binding of [ 125I ] labeled urokinase type plasminogen activator ( u PA ) was studied on human mesangial cells ( MC ) in culture . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Heparin and heparan sulfate enhancement of the inhibitory activity of plasminogen activator inhibitor type 1 toward urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Bovine vascular smooth muscle cells ( SMC ) express the urokinase type plasminogen activator receptor ( u PAR ) claimed to be important in cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast to TNF alpha induced PAI 1 production , the transcription and synthesis of urokinase type plasminogen activator ( u PA ) was not inhibited by genistein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TPA induced only slight reductions , whereas retinoic acid and doxorubicin caused an increase in invasiveness , enzymatic activity and differentiation in the clone showing low invasivity , low urokinase type plasminogen activator levels and high differentiation . ^^^ In contrast , in the clone showing high invasivity , high urokinase type plasminogen activator levels and low differentiation it was found that : TPA was ineffective ; retinoic acid induced a reduction of plasminogen activator but no modifications of invasiveness and differentiation ; doxorubicin caused a decrease in invasiveness and plasminogen activator activity but no modification of morphological features . ^^^ The results reported here indicate that in the presence of these drugs the associations between invasiveness and urokinase type plasminogen activator activity and between invasiveness and differentiation are lost . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Replacement of the native P 14 Thr 333 residue by an Arg ( Thr 333 > Arg ) resulted in complete loss of inhibitory activity toward tissue type plasminogen activator and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The two subunits of a phospholipase A 2 inhibitor from the plasma of Thailand cobra having structural similarity to urokinase type plasminogen activator receptor and LY 6 related proteins . ^^^ These sequence units were found to be significantly homologous to those of urokinase type plasminogen activator receptor and of Ly 6 related proteins , such as Ly 6A / E , Ly 6C , ThB , and CD 59 antigens . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma D dimers ( one ELISA and two latex assays ) , fibrin monomer , prothrombin fragment 1 + 2 ( F1+2 ) , thrombin antithrombin 3 complex ( TAT ) and the t PA PAI 1 complex were all significantly correlated to the extension of DVT , whilst fibronectin , tissue type plasminogen activator ( t PA ) , single chain urokinase type plasminogen activator ( scru PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were not . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When mRNA obtained from RAECs cultured for 16 hours in serum free medium was analysed for the presence of specific messages of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) using human probes , weak specific binding could be seen for both t PA and mu PA while the PAI 1 probe gave a strong specific signal at 3 . 4 Kb and a weak signal at 2 . 3 Kb . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They had high predialysis plasma concentrations of endothelium derived glycoproteins : von Willebrand factor , tissue type plasminogen activator and urokinase type plasminogen activator , which are secreted by endothelial cells , but also soluble thrombomodulin , a marker of endothelial cell injury . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast , no change in urokinase type plasminogen activator and plasminogen activator inhibitor 1 ( PAI 1 ) antigen synthesis was seen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We show that interleukin 1 beta and tumor necrosis factor alpha increase urokinase type plasminogen activator production , reinforcing the concept that increased urokinase production is associated with inflammatory processes . ^^^ Among the inflammatory agents used , transforming growth factor beta was found to be the most effective modulator of both urokinase type plasminogen activator and plasminogen activator inhibitor type 1 , inducing severalfold increases of activity of urokinase type plasminogen activator , antigen and the corresponding mRNA and increasing plasminogen activator inhibitor type 1 antigen and mRNA levels . ^^^ Urokinase type plasminogen activator and plasminogen activator inhibitor type 1 modulation by transforming growth factor beta may play a critical role in hepatic pathophysiology . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human colorectal carcinogenesis has been shown previously to be associated with impressive changes in the tissue levels of plasminogen activators and their inhibitors , exemplified by an increase in the urokinase type plasminogen activator ( u PA ) and the inhibitors PAI 1 and PAI 2 , and a decrease in tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Incubation of PAI 1 P 12 , PAI 1 P 10 , or PAI 1 P 8 with a 2 fold molar excess of urokinase type plasminogen activator , plasmin , or thrombin also primarily generated a 41 kDa cleavage product ( 62 89 % ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The structure of the urokinase type plasminogen activator receptor gene . ^^^ The cellular receptor for urokinase type plasminogen activator ( uPAR ) is a glycosylphosphatidylinositol ( GPI ) anchored membrane protein that plays a central role in pericellular plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied urokinase type plasminogen activator ( u PA ) dependent chemotaxis and DNA synthesis in both human fibroblasts and LB 6 mouse fibroblasts transfected with human u PA receptor ( u PAR ) gene ( LB 6 clone 19 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation of thrombin inactivated single chain urokinase type plasminogen activator by dipeptidyl peptidase 1 ( cathepsin C ) . ^^^ Single chain urokinase type plasminogen activator ( scu PA ) is inactivated by thrombin , which cleaves the peptide bond between Arg 156 and Phe 157 . ^^^ In a search for potential activators of thrombin cleaved two chain urokinase type plasminogen activator ( tcu PA / T ) , we found that the lysosomal aminopeptidase dipeptidyl peptidase 1 or cathepsin C efficiently activates tcu PA / T . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interferon alpha 2 counteracts interleukin 1 alpha stimulated expression of urokinase type plasminogen activator in human foreskin microvascular endothelial cells in vitro . ^^^ We investigated the effect of interferon alpha 2 ( IFN alpha 2 ) on interleukin 1 alpha ( IL 1 alpha ) induced up regulation of urokinase type plasminogen activator ( u PA ) expression in human foreskin microvascular endothelial cells ( HFMEC ) and human umbilical vein endothelial cells ( HUVEC ) in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Northern blot analysis showed that messenger ribonucleic acid specific for urokinase and plasminogen activator inhibitor 1 was present in colonic crypt cells and that expression over 18 hours of culture was increased 12 fold for urokinase type plasminogen activator and two to fourfold for the inhibitor compared with values found in autologous freshly isolated cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression and localization of urokinase type plasminogen activator receptor in human gliomas . ^^^ Urokinase type plasminogen activator receptors ( uPARs ) play an important role in tumor invasion by localizing degradative enzymes at the invasive zone . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vitro urokinase type plasminogen activator levels and total plasminogen activator activity in squamous cell carcinomas of the head and neck . ^^^ OBJECTIVE : Determine total plasminogen activator ( PA ) activity and urokinase type plasminogen activator ( u PA ) levels in cell free supernatants derived from primary and metastatic squamous cell carcinoma of the head and neck . ^^^ Urokinase type plasminogen activator levels were measured with enzyme linked immunosorbent assay technique . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cells from subretinal fluid had more proteolytic activity and secreted more active urokinase type plasminogen activator than retinal pigment epithelial cells of donor origin . ^^^ It is possible that cells from subretinal fluid are modulated by the conditions in subretinal fluid to secrete more urokinase type plasminogen activator , generally considered to be a sign of invasive growth or that the subretinal fluid cultures contain other cell types than solely retinal pigment epithelial cells , such as pigmented macrophages , with an active plasminogen activator production . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activity was elevated in the RA treated tissues and this was due to increased amounts of both urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The antithrombotic effects of rabbit polyclonal inhibitory antibodies to rat collagen type 1 3 and of murine non inhibitory monoclonals to human recombinant single / two chain urokinase type plasminogen activator ( rscu / rtcu PA ) , cross reacting with rat scu / tcu PA and their chemically synthesized conjugate , were studied both in vitro and in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concomitant augmentation of urokinase type plasminogen activator and collagenase like proteinase activities in 10 ray irradiated cells of a human metastatic carcinomatous line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mechanism of the enhanced intrinsic activity of single chain urokinase type plasminogen activator during ongoing fibrinolysis . ^^^ The activation of plasminogen at the surface of fibrin by single chain urokinase type plasminogen activator ( scu PA ) was investigated using recombinant forms of a plasmin resistant mutant of scu PA , rscu PA Glu 158 , and an inactive catalytic site mutant of human plasminogen , rPg Ala 741 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Solution structure of the kringle domain from urokinase type plasminogen activator . ^^^ The solution structure of the kringle domain from urokinase type plasminogen activator ( u PA ) has been determined using 1H nuclear magnetic resonance spectroscopy and dynamical simulated annealing calculations . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In five children with HUS we performed a prospective study of plasminogen activator activity and two plasminogen activator antigens : tissue type plasminogen activator and urokinase type plasminogen activator before and after intravenous desmopressin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical localization of urokinase type plasminogen activator and the plasminogen activator inhibitors 1 and 2 in early human implantation sites . ^^^ OBJECTIVE : Our purpose was to immunolocalize urokinase type plasminogen activator and the plasminogen activator inhibitors 1 and 2 in human implantation sites , with emphasis on the types of trophoblast expressing the plasminogen activator and the inhibitors . ^^^ CONCLUSIONS : Urokinase type plasminogen activator and the plasminogen activator inhibitors 1 and 2 were localized in all three forms of trophoblast at the maternal fetal interface in early human implantation sites , particularly the differentiated and invasive forms of trophoblast . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The chimeric activator , 59D8 scuPA T , was made from the Fab fragment of an anti fibrin antibody ( 59D8 ) and a C terminal portion of a thrombin activable low molecular weight single chain urokinase plasminogen activator , scuPA T , obtained by deletion of Phe 157 and Lys 158 from low molecular weight single chain urokinase type plasminogen activator ( scuPA ) by site directed mutagenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of the p 26 mJUN protein blocked both constitutive and inducible transcriptional trans activation of several AP 1 responsive reporter chloramphenicol acetyltransferase constructs . p 26 mJUN was able to block both 12 O tetradecanoylphorbol 13 acetate ( TPA ) and okadaic acid induced expression of the mouse stromelysin gene in 10Gy5 cells and TPA induced expression of the urokinase type plasminogen activator gene in PDV cells as determined by Northern analyses . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As reported in recent literature , heparin stimulated the activation of Lys plasminogen by high molecular weight ( HMW ) and low molecular weight ( LMW ) two chain urokinase type plasminogen activator ( u PA ) and two chain tissue type plasminogen activator ( t PA ) 10 to 17 fold . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURES Comparisons of concentrations of tissue plasminogen activator , urokinase type plasminogen activator , tissue plasminogen activator inhibitor 1 , cross linked fibrin degradation products , von Willebrand factor , and thrombin antithrombin 3 complexes in patients and controls at admission ; same comparisons in patients with silent ischaemia at the start of an episode and 10 minutes later . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Unfolding studies of the protease domain of urokinase type plasminogen activator : the existence of partly folded states and stable subdomains . ^^^ The domain structure and the stability against thermal and chemical denaturation of urokinase type plasminogen activator ( u PA ) have been investigated by NMR spectroscopy and differential scanning calorimetry ( DSC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both interferons caused a marked decrease in PAI 1 mRNA expression after 48 h with no change in urokinase type plasminogen activator ( u PA ) mRNA expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In both groups , plasma levels of tissue type plasminogen activator and urokinase type plasminogen activator before the operation were higher than those 15 days after operation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , steady state transcript levels of the tumor associated AP 1 responsive genes stromelysin , urokinase type plasminogen activator , c jun , and c fos were higher in malignant 308 10Gy5 cells than in benign 308 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Parathyroid hormone ( PTH ) , prostaglandin E 2 , ( PGE 2 ) or tumor necrosis factor alpha ( TNF alpha ) enhanced the secretion of urokinase type plasminogen activator ( u PA ) antigen and suppressed the secretion of plasminogen activator inhibitor 1 ( PAI 1 ) antigen to the conditioned medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase type plasminogen activator is expressed by keratinocytes at the leading edge during re epithelialization of mouse skin wounds . ^^^ Expression of urokinase type plasminogen activator receptor mRNA was examined in vivo in mouse skin wounds by in situ hybridization . ^^^ Urokinase type plasminogen activator receptor mRNA was found in keratinocytes at the front of the regenerative epithelial outgrowths at the edge of 12 , 48 , and 96 hour old wounds . ^^^ Using in situ hybridization , no cells expressing urokinase type plasminogen activator receptor mRNA could be detected in normal epidermis , in normal looking epidermis adjacent to the wounds , in dermis , in subcutis , or in newly formed granulation tissue . ^^^ Together with previous findings on expression of urokinase type plasminogen activator and its type 1 inhibitor , the localized and regulated expression of urokinase type plasminogen activator receptor mRNA during skin wound healing indicates that focal extracellular proteolysis at the cell surface generated by receptor bound urokinase type plasminogen activator is implicated in the migration of re epithelializing keratinocytes . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In zymographic analysis , 23 of the 25 samples showed positive reactions for enzyme activities comigrating with plasmin and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Physical association of complement receptor type 3 and urokinase type plasminogen activator receptor in neutrophil membranes . ^^^ This study tests the possible physical interactions of another extensively glycosylated glycosyl phosphatidylinositol linked protein , the urokinase type plasminogen activator receptor ( uPAR ) , with CR 3 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Solution structure of the amino terminal fragment of urokinase type plasminogen activator . ^^^ The amino terminal fragment ( ATF ) of urokinase type plasminogen activator is a two domain protein which consists of a growth factor and a kringle domain . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We measured thrombin with a chromogenic substrate at several time intervals after recalcification of citrated plasma which had been preincubated with urokinase , streptokinase , recombinant tissue plasminogen activator ( rt PA ) or recombinant single chain urokinase type plasminogen activator ( rscu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression and characterization of clustered charge to alanine mutants of low M ( r ) single chain urokinase type plasminogen activator . ^^^ In an effort to modify the fibrinolytic and / or pharmacokinetic properties of recombinant low M ( r ) single chain urokinase type plasminogen activator ( rscu PA 32k ) , mutants were prepared by site directed mutagenesis of clusters of charged amino acids with the highest solvent accessibility . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of c fos gene expression by urokinase type plasminogen activator in human ovarian cancer cells . ^^^ Binding of urokinase type plasminogen activator ( u PA ) to u PA receptor ( u PAR ) induces the rapid and transient expression of c fos in OC 7 ovarian carcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
No urokinase type plasminogen activator antigen was detected by urokinase type plasminogen activator immunoassay . ^^^ Fibrin autography exhibited lytic activity at 64 kDa and 54 kDa attributable to tissue type plasminogen activator and urokinase type plasminogen activator . ^^^ There was only a small amount of lysis secondary to urokinase type plasminogen activator . ^^^ In contrast to occlusive disease , aneurysms had an inflammatory cell infiltrate characterized by the expression of urokinase type plasminogen activator by specific mononuclear cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vitro , Ets 1 proteins are involved in the transcriptional induction of genes such as stromelysin 1 , collagenase 1 or urokinase type plasminogen activator , which are proteases responsible for extracellular matrix degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , a member of the serpin family of serine protease inhibitors , inhibits both tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have studied the expression of the urokinase type plasminogen activator and the urokinase type plasminogen activator cell surface receptor in xenografts of human MDA MB 231 mammary carcinoma cells growing invasively in nude mice . ^^^ Northern analysis showed the presence of both human and mouse urokinase type plasminogen activator and urokinase type plasminogen activator receptor mRNA in tumor extracts . ^^^ By in situ hybridization , mRNA for human urokinase type plasminogen activator and its receptor was detected in virtually all the cancer cells , while mouse urokinase type plasminogen activator and urokinase type plasminogen activator receptor mRNA was expressed by tumor infiltrating fibroblast like and macrophage like cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zymographic analyses showed that tissue type plasminogen activator ( t PA ) occurred in association with casein micelles , partially as a complex with type 1 plasminogen activator inhibitor ( PAI 1 ) , whereas urokinase type plasminogen activator ( u PA ) was confined to milk leukocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Forty one patients with acute myocardial infarction were treated with a combination of recombinant tissue type plasminogen activator and human single chain urokinase type plasminogen activator . ^^^ The results demonstrate that high levels of Lp ( a ) do not influence thrombolysis in patients with acute myocardial infarction when low dose pharmacologic concentrations of recombinant tissue type plasminogen activator and human single chain urokinase type plasminogen activator are applied in combination . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Rat astrocytes synthesize and secrete two types of plasminogen activators ( PAs ) , tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) , whose functions are related to cell proliferation , migration , and differentiation during development . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The high affinity receptor that binds human urokinase type plasminogen activator ( hu PAR ) is a glycosyl phosphatidylinositol ( GPI ) anchored cell surface glycoprotein that belongs to the Ly 6 superfamily of T cell activating receptors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Six other patients had a poor urokinase type plasminogen activator reactivity to venous occlusion ( post stasis levels lower than 0 . 18 ng / ml ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
K2tu PA is a hybrid plasminogen activator linking the kringle 2 domain of tissue type plasminogen activator ( t PA ) to the catalytic protease domain of single chain urokinase type plasminogen activator ( scu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Pharmacokinetics of saruplase , a recombinant unglycosylated human single chain urokinase type plasminogen activator and its effects on fibrinolytic and haemostatic parameters in healthy male subjects . ^^^ Pharmacokinetics of two doses of the recombinant single chain urokinase type plasminogen activator ( r scu PA ) saruplase ( 40 and 20 mg ) and its effect on fibrinolytic and haemostatic parameters were studied in six healthy male subjects using a randomized , double blind , placebo controlled , cross over study . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type and urokinase type plasminogen activator activity ( expressed as mIU / mg protein ) and plasminogen activator inhibitor type 1 antigen were assayed in homogenates of gastric and duodenal biopsy specimens taken from patients with : normal endoscopy ( controls ) ( n = 14 ) ; active duodenal ulcer ( n = 21 ) ; healed duodenal ulcer ( n = 12 ) ; and active benign gastric ulcer ( n = 15 ) . ^^^ In controls mean duodenal tissue type plasminogen activator activity was 4110 and urokinase type plasminogen activator activity 150 ; gastric tissue type plasminogen activator was 2760 and urokinase type plasminogen activator 170 ; plasminogen activator inhibitor type 1 was generally undetectable . ^^^ At the edge of active duodenal ulcers tissue type plasminogen activator was considerably reduced , 2220 ( p < 0 . 001 ) whereas urokinase type plasminogen activator was raised , 290 ( p < 0 . 01 ) . ^^^ At the edge of active benign gastric ulcers tissue type plasminogen activator was substantially reduced , 1160 ( p < 0 . 001 ) but urokinase type plasminogen activator was unchanged . ^^^ At the scar of healed duodenal ulcers tissue type plasminogen activator was slightly reduced , 3290 , but urokinase type plasminogen activator was increased , 308 ( p < 0 . 05 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transcription factors which recognize both the SV 40 promoter and the proximal promoter region of the human urokinase type plasminogen activator ( h uPA ) gene are present in nuclear extracts from primary cultures of mouse Sertoli cells ; prolonged ( more than 12 h ) ( Bu ) 2cAMP stimulation of Sertoli cells induces the formation of different specific DNA protein complexes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The content of urokinase type plasminogen activator and tumor recurrence in superficial bladder cancer . ^^^ The contents of urokinase type plasminogen activator were estimated in superficial bladder cancer from 42 patients undergoing transurethral resection for the first time . ^^^ Tumor recurred in 11 of 16 patients ( 68 . 8 % ) with high urokinase type plasminogen activator content ( 8 ng . urokinase type plasminogen activator per mg . protein and more ) and in 12 of 26 ( 46 . 2 % ) with low urokinase type plasminogen activator content . ^^^ The progression rates in the high urokinase type plasminogen activator group with stage pTa disease or tumors more than 1 cm . large were significantly higher than those in the low urokinase type plasminogen activator group ( p < 0 . 05 ) . ^^^ In conclusion , urokinase type plasminogen activator content may be a useful marker to predict recurrence and progression of superficial bladder cancer . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparison of the in vitro fibrinolytic activities of low and high molecular weight single chain urokinase type plasminogen activator . ^^^ The fibrinolytic activity of low molecular weight ( LMW ) single chain urokinase type plasminogen activator ( scu PA ) lacking the epidermal growth factor domain and the kringle domain was compared with the activity of high molecular weight ( HMW ) scu PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator expression by neurons and oligodendrocytes during process outgrowth in developing rat brain . ^^^ Thus for both neurons and oligodendrocytes , the onset of urokinase type plasminogen activator expression coincides with their initiation of major process outgrowth , although neurons maintain this expression in the adult , possibly to retain a degree of synaptic plasticity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Coronary thrombolysis with K1K2Pu , a chimeric tissue type and urokinase type plasminogen activator : a feasibility study in six patients with acute myocardial infarction . ^^^ BACKGROUND : K1K2Pu is a recombinant chimeric tissue type and urokinase type plasminogen activator consisting of the two kringle domains ( K 1 and K 2 ) of human tissue type plasminogen activator ( t PA ) and the serine proteinase domain ( Pu ) of single chain urokinase type plasminogen activator ( scu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Several newer drugs with theoretical advantages over older drugs such as single chain urokinase type plasminogen activator or K1K2PU are currently undergoing trials . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Specifically , the following components were examined : the enzymes urokinase type plasminogen activator and tissue type plasminogen activator and their type 1 and type 2 inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Other factors examined ( including the laminin receptor , cathepsin B , cathepsin L , urokinase type plasminogen activator , and tissue inhibitor metalloproteinases ) are expressed by both invasive and noninvasive gastric cancer cells , whereas collagenase type 4 92 kD form is not expressed by any of the cells examined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding activity is species and protein specific in that neither mouse urokinase type plasminogen activator ( u PA ) nor human t PA bind to cultured morulae . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Double chain t PA is as efficient as single chain t PA in stimulating smooth muscle cell mitogenesis , whereas single chain urokinase type plasminogen activator ( u PA ) or u PA and plasmin or plasminogen are ineffective . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recent findings have elucidated the mechanism for clearance from the extracellular space of the two types of plasminogen activators , urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) , and their type 1 inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator and plasminogen activator inhibitor 2 in gastric carcinoma ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Elevated levels of urokinase type plasminogen activator and plasminogen activator inhibitor type 1 in malignant human brain tumors . ^^^ Quantitative zymography and enzyme linked immunosorbent assay were used to determine the amounts of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator , and plasminogen activator inhibitors type 1 and type 2 ( PAI 1 and PAI 2 ) in benign and malignant primary brain tumors ( n = 28 ) as well as nonneoplastic brain ( n = 5 ) . u PA and PAI 1 antigen were undetectable in normal brain but significantly elevated in glioblastoma multiforme ( u PA , 2 . 86 + / 3 . 01 ng / mg ; PAI 1 , 8 . 19 + / 5 . 57 ng / mg ; P < 0 . 001 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effects of macrophage colony stimulating factor on human monocytes : induction of expression of urokinase type plasminogen activator , but not of secreted prostaglandin E 2 , interleukin 6 , interleukin 1 , or tumor necrosis factor alpha . ^^^ We demonstrate with elutriation purified human monocytes that , in contrast to lipopolysaccharide , recombinant human CSF 1 does not induce secretion of prostaglandin E 2 , interleukin 6 ( IL 6 ) , IL 1 beta , or tumor necrosis factor alpha , as measured by immunoassay ; however , increased urokinase type plasminogen activator ( u PA ) activity in cell lysates and mRNA was observed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although the vasopressin analogue desamino d arginine vasopressin ( DDAVP ) induces a very well characterized increase in factor 8 ( FVIII ) , von Willebrand factor ( vWF ) , tissue plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) , the mechanism ( s ) by which DDAVP enhances the plasma levels of these proteins is poorly understood . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , urokinase type plasminogen activator was detected immunohistochemically in 11 . 1 % of the defective areas , and in none of the continuous areas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Correlation between tumor proliferation and tumor tissue level of urokinase type plasminogen activator . ^^^ We established a xenotransplanted human colon cancer strain , TK 3 , which produces urokinase type plasminogen activator ( U PA ) and carcinoembryonic antigen ( CEA ) simultaneously . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : Our purpose was to define the mode of interaction of tumor cells in ovarian carcinoma with both the coagulation ( procoagulant initiated ) and fibrinolysis ( urokinase type plasminogen activator initiated ) ( u PA ) pathways . ^^^ RESULTS : The ovarian tumor cells expressed urokinase type plasminogen activator in a pattern that was variable in intensity and distribution . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor 1 , and plasminogen activator inhibitor 2 were also measured by means of enzyme linked immunosorbent assays . ^^^ Low levels of urokinase type plasminogen activator were found in 5 of 10 patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Under baseline conditions , the latter parameter was correlated as well with the antigen concentration of urokinase type plasminogen activator ( u PA ; r = 0 . 503 , P < 0 . 002 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
No significant changes in plasma D dimer and fibrinogen values were detected in the two groups . tPA , urokinase type plasminogen activator , PAI 1 and procoagulant activity were evaluated in vitro on cultured human endothelial cells and found to be unchanged following GM CSF addition . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
When plasminogen was added to cultured keratinocytes it was activated by cell associated urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator production by endothelial cells and vascular smooth muscle cells was undetectable in control medium and was augmented to similar levels in response to plaque macrophage and monocyte conditioned media . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Treatment of the plasminogen activators tissue plasminogen activator and two chain urokinase type plasminogen activator , as well as trypsin , neutrophil elastase , and thrombin with Cu ( 2 ) and ascorbate resulted in a loss of their amidolytic and proteolytic activity , indicating the general susceptibility of serine proteases to this type of oxidation . ^^^ Oxidation of the zymogens Glu plasminogen and single chain urokinase type plasminogen activator by Cu ( 2 ) and ascorbate resulted in the failure of these molecules to generate active enzymes when treated with plasminogen activators or plasmin , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Extrapolation of these in vitro results to periimplantational events in humans suggests that under progesterone regulation , decidual cell derived PAI 1 could 1 ) restrain blastocyst invasion of the stroma by inhibiting trophoblast associated urokinase type plasminogen activator , and 2 ) prevent hemorrhage during trophoblast invasion of the endometrial vasculature by inhibiting fibrinolysis mediated by tissue type plasminogen activator . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As part of a prospective randomized double blind trial ( PRIMI ) to study the early patency rate of the infarct related artery after saruplase ( INN for recombinant unglycosylated full length human single chain urokinase type plasminogen activator ) vs streptokinase in a subgroup of patients , left ventricular function was compared between both treatment groups at 90 min and 24 h after thrombolysis and at discharge , and ventricular function was related to the coronary perfusion grade . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NMR studies of the dynamics of the multidomain protein urokinase type plasminogen activator . u PA ( urokinase type plasminogen activator or urokinase ) has been studied under a variety of solution conditions by 1 D and 2 D NMR spectroscopy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied non mitogenic signal transduction in a human cell line of epidermal origin which is induced to chemotaxis following stimulation with human urokinase type plasminogen activator ( u PA ) or with the amino terminal fragment ( ATF ) of u PA A chain , which specifically interacts with the cellular receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inactivation of high and low molecular weight single chain urokinase type plasminogen activator ( pro urokinase ) by thrombin in the presence of thrombomodulin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of the urokinase type plasminogen activator to its cell surface receptor is inhibited by low doses of suramin . ^^^ The multipotent drug suramin , which is currently being studied as an anticancer agent , was found to inhibit the interaction between the urokinase type plasminogen activator ( u PA ) and its cellular receptor . 50 % inhibition of binding was obtained with a suramin concentration between 30 and 60 micrograms / ml when using U 937 cells and a ligand concentration of 0 . 3 nM . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) , its receptor ( u PAR ) , and type 1 inhibitor ( PAI 1 ) in cultured human mesangial cells were investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Role of the glycosaminoglycan component of thrombomodulin in its acceleration of the inactivation of single chain urokinase type plasminogen activator by thrombin . ^^^ The inactivation of single chain urokinase type plasminogen activator ( scu PA ) by thrombin is accelerated about 70 fold by TM [ De Munk , Groeneveld and Rijken ( 1991 ) J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Functional effects of kringle 2 glycosylation in a hybrid plasminogen activator . uK2t PA is a hybrid plasminogen activator in which the epidermal growth factor like domain of the urokinase type plasminogen activator precedes the kringle 2 and catalytic domains of tissue type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Bovine urokinase type plasminogen activator and its receptor : cloning and induction by retinoic acid . ^^^ Full length cDNAs encoding bovine urokinase type plasminogen activator ( u PA ) and urokinase receptor ( u PAR ) were cloned from an aortic endothelial cell cDNA library using PCR amplified cDNA fragments as probes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Histological samples from 60 invasive ductal breast carcinomas were investigated for immunoreactivity for the receptor for urokinase type plasminogen activator ( uPAR ) with the use of two monoclonal antibodies recognizing different epitopes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of urokinase type plasminogen activator expression in squamous cell carcinoma of the oral cavity . ^^^ We undertook a study to determine the role and regulation of expression of urokinase type plasminogen activator in squamous cell carcinoma of the oral cavity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Modulation of receptor bound urokinase type plasminogen activator on human monocytes by non steroidal antiinflammatory drugs . ^^^ Studies on the modulation of receptor bound urokinase type plasminogen activator on peripheral blood monocytes of patients with RA showed that tenoxicam , a non steroidal antiinflammatory drug with long half life and good tissue permeability , is able to downregulate the total number of urokinase receptors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At the time of fertilization both murine gametes express plasminogen dependent proteolytic activity : unfertilized eggs secrete tissue type plasminogen activator and ejaculated spermatozoa have urokinase type plasminogen activator bound to their surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two hybrid plasminogen activators ( K2tu PA and FK2tu PA ) , linking the kringle 2 domain or the finger plus the kringle 2 domains of tissue type plasminogen activator ( t PA ) to the catalytic domain of single chain urokinase type plasminogen activator ( scu PA ) were studied . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One of the phenotypic hallmarks of migrating endothelial cells , both in vivo and in vitro , is expression of the urokinase type plasminogen activator ( u PA ) , a key mediator of extracellular proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Because thrombomodulin is known to inhibit single chain urokinase type plasminogen activator , a molecule present in the osteoblast microenvironment , these findings suggest that thrombomodulin could play a role in the regulation of bone resorption by modulating the plasmin system . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for plasmin and the receptor for urokinase type plasminogen activator were characterized on MCF 7 cells either independently or simultaneously , using fluorescence microscopy and confocal microscopy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Nerve growth factor gamma activates soluble and receptor bound single chain urokinase type plasminogen activator . ^^^ In this study , we demonstrated that NGF gamma cleaves recombinant single chain urokinase type plasminogen activator ( scu PA ) , converting the zymogen into a two chain form ( tcu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Localization of the disulfide bonds in the NH 2 terminal domain of the cellular receptor for human urokinase type plasminogen activator . ^^^ The receptor for human urokinase type plasminogen activator ( uPAR ) is synthesized as a 313 residue long polypeptide containing 28 cysteine residues , the pattern of which defines three homologous repeats within the protein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vitro activation of pro cathepsin B by three serine proteinases : leucocyte elastase , cathepsin G , and the urokinase type plasminogen activator . ^^^ The urokinase type plasminogen activator activated pro cathepsin B faster than leucocyte proteinases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator antigen as a prognostic factor in bladder cancer ] . ^^^ The relationship between the urokinase type plasminogen activator ( u PA ) content in extracts of cancer tissues and the histological grade or the extent of the primary tumor as prognostic factors in bladder cancer were examined in 55 cases of bladder cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Carcinogenesis in the human colon is associated with a marked increase of urokinase type plasminogen activator and a decrease of tissue type plasminogen activator . ^^^ This study was performed to determine the concentrations of urokinase type plasminogen activator and tissue type plasminogen activator in normal tissue and carcinomas along the upper part of the gastrointestinal tract . ^^^ Although the concentrations of tissue type plasminogen activator and urokinase type plasminogen activator in normal squamous epithelium of the oesophagus were low compared with those in columnar epithelium from the stomach , the urokinase type plasminogen activator / tissue type plasminogen activator antigen ratio of the different locations showed hardly any difference . ^^^ Significant but heterogeneous increases were found in urokinase type plasminogen activator concentrations of biopsy specimens originating from carcinomas of both epithelial cell types . ^^^ The increase of urokinase type plasminogen activator and urokinase type plasminogen activator / tissue type plasminogen activator antigen ratio and the decrease of tissue type plasminogen activator in the carcinomas did not show a significant correlation with known prognostic determinants as differentiation grade , TNM classification , intestinal metaplasia , inflammation , and ulceration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) were identified in the ECM by fibrin zymography , immunoblotting , and inhibition of plasminogen activation by anti u PA and anti t PA antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The kinetics of clot binding of two human fibrin specific monoclonal antibodies ( MA 12B3 and MA 15C5 ) and of clot lysis with their chemical 1 : 1 stoichiometric complexes with recombinant single chain urokinase type plasminogen activator ( rscu PA ) ( rscu PA / MA 12B3 and rscu PA / MA 15C5 ) were determined in hamsters and rabbits . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Evolution of urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) in orthotopic liver transplantation ( OLT ) . ^^^ Originally , tissue type plasminogen activator ( t PA ) was considered to be responsible for the increases , but the efficacy of aprotinin which inhibits besides plasmin also kallikrein and urokinase type plasminogen activator ( u PA ) suggested also a role for the intrinsic and contact system dependent plasminogen activators . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The tissue plasminogen activator finger domain confers fibrin dependent enhancement of catalytic activity to single chain urokinase type plasminogen activator . ^^^ To determine whether the fibrin binding domains of tissue plasminogen activator ( tPA ) can confer enhanced catalytic activity to single chain urokinase type plasminogen activator ( scuPA ) , we constructed , expressed , and characterized the kinetics of five recombinant tPA / scuPA hybrid molecules . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , we observed a stimulation of urokinase type plasminogen activator ( u PA ) associated with monocytes when these cells were incubated with lymphocytes for 18 h at 37 degrees C . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Clinical value of determination of urokinase type plasminogen activator antigen in plasma for detection of colorectal cancer : comparison with circulating tumor associated antigens CA 19 9 and carcinoembryonic antigen . ^^^ We determined urokinase type plasminogen activator antigen ( u PA ) , gastrointestinal cancer associated antigen ( CA 19 9 ) , and carcinoembryonic antigen ( CEA ) in the plasma of patients with colorectal cancer at the time of clinical tumor detection and in a group of patients with Crohn ' s disease and analyzed the specificity of these tumor markers . u PA , CA 19 9 , and CEA were indicative for colorectal cancer in 75 . 5 % , 51 . 5 % , and 51 . 5 % of tumor patients , respectively , with a specificity of 79 . 3 % , 94 % , and 97 . 5 % . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Blood samples were obtained on days 1 , 4 , and 7 after hospital admission to measure tissue type plasminogen activator antigen ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor antigen ( PAI 1 ) , plasminogen , alpha 2 antiplasmin , fibrinogen , antithrombin 3 , protein C , protein S , thrombin antithrombin complexes ( TAT ) , D dimer , and von Willebrand factor related antigen ( vWF : Ag ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Retinoids increase urokinase type plasminogen activator production by human retinal pigment epithelial cells in culture . ^^^ Both retinoids caused a twofold to sevenfold increase in urokinase type plasminogen activator in the medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interleukin 4 stimulates expression of urokinase type plasminogen activator in cultured human foreskin microvascular endothelial cells . ^^^ Only foreskin microvascular endothelial cells ( EC ) responded to IL 4 treatment with a dose and time dependent increase in urokinase type plasminogen activator ( u PA ) ( control : 3 . 0 + / 0 . 8 ng / 10 ( 5 ) cells / 24 h ; 200 U / mL IL 4 : 6 . 7 + / 0 . 8 ng / 10 ( 5 ) cells / 24 h ) , whereas human macrovascular EC remained unaffected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enhancement of in vitro cytotoxic activity of tumor necrosis factor alpha ( TNF alpha ) was observed in combination with lysosome labilizers , particularly with urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) and lipoprotein lipase ( LPL ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A chimeric protein ( rscu PA 40 kDa / Hir ) , consisting of the C terminal amino acids 53 65 of hirudin ( Hir ) , fused via a 14 amino acid linker sequence to the C terminal of a 40 kDa fragment ( Ser 47 Leu411 ) of recombinant ( r ) single chain ( sc ) urokinase type plasminogen activator ( rscu PA ) , was produced by expression of the corresponding chimeric cDNA in Escherichia coli cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator induced monocyte adhesion requires a carboxyl terminal lysine and cAMP dependent signal transduction . ^^^ Urokinase type plasminogen activator ( u PA ) or its amino terminal fragment ( ATF ) containing the u PA receptor ( u PAR ) binding domain , is known to promote monocyte adhesion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of urokinase type plasminogen activator expression by the 5 mos oncogene . ^^^ We undertook a study to determine if the serine threonine kinase encoding 5 mos oncogene regulated the expression of the urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mice with combined homozygous deficiency of tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) ( T U ) , of t PA and plasminogen activator inhibitor 1 ( PAI 1 ) ( T P ) , of u PA and PAI 1 ( U P ) or of t PA , u PA , and PAI 1 ( T U P ) were generated by inbreeding of mice with the respective deficiencies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prognostic value of urokinase type plasminogen activator in patients with superficial bladder cancer . ^^^ A number of studies have demonstrated that urokinase type plasminogen activator ( u PA ) is one of the major enzymes in the development of cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activity and antigen , euglobulin fibrinolytic activity , tissue type plasminogen activator activity and antigen urokinase type plasminogen activator antigen , plasminogen activator inhibitor 1 activity and antigen , plasminogen activator inhibitor 2 antigen , tissue type plasminogen activator / plasminogen activator inhibitor complexes , alpha 2 antiplasmin , histidine rich glycoprotein , and fibrinogen / fibrin degradation products were measured in blood samples taken from the umbilical vein of 100 healthy full term newborns . ^^^ We also observed high tissue type plasminogen activator activity levels ( P < 0 . 001 ) , whereas urokinase type plasminogen activator antigen levels were lower than in adults . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of the matrix metalloproteinases gelatinase A and stromelysin 1 and the serine proteinase urokinase type plasminogen activator , which was low during lactation , was strongly upregulated in parallel starting at day 4 after weaning , coinciding with start of the collapse of the lobulo alveolar structures and the intensive tissue remodeling in involution . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Diagnostic efficacy of plasma urokinase type plasminogen activator and plasminogen activator inhibitor 2 in differentiation of hepatocellular carcinoma from cirrhosis . ^^^ We determined the plasma antigen levels of urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor 2 ( PAI 2 ) in 41 patients with hepatocellular carcinoma and 28 patients with different stages of liver cirrhosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The parasite derived serine protease is not inhibited by amiloride which is a strong inhibitor of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two physiological plasminogen activators have been identified : tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Agents available for clinical use are : the physiologic tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) either in a single chain ( scu PA , prourokinase ) or a two chain ( tcu PA , urokinase ) form , and the bacterial activator plasminogen streptokinase or its anisoylated complex with plasminogen ( APSAC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : Urokinase type plasminogen activator and its inhibitor plasminogen activator inhibitor are associated with invasion and formation of metastases in tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The crystal structure of the catalytic domain of human urokinase type plasminogen activator . ^^^ BACKGROUND : Urokinase type plasminogen activator ( u PA ) promotes fibrinolysis by catalyzing the conversion of plasminogen to the active protease plasmin via the cleavage of a peptide bond . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor reversibly dissociates from complement receptor type 3 ( alpha M beta 2 ' CD11b / CD18 ) during neutrophil polarization . ^^^ Previous studies have shown that the leukocyte integrin CR 3 ( CD11b / CD18 ) is physically associated with the urokinase type plasminogen activator receptor ( uPAR ; CD 87 ) , a glycosyl phosphatidylinositol ( GPI ) linked protein , in resting neutrophil membranes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Three enzyme linked immunosorbent assays for the quantitation of murine tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , were developed using monoclonal antibodies raised against the autologous proteins in gene inactivated mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mice homozygously deficient for the urokinase type plasminogen activator ( u PA ) receptor ( u PAR 1 ) were generated by homologous recombination in D 3 , embryonic stem cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparative study of four extraction procedures for urokinase type plasminogen activator and plasminogen activator inhibitor 1 in breast cancer tissues . ^^^ The urokinase type plasminogen activator ( u PA ) and the plasminogen activator inhibitor 1 ( PAI 1 ) are among the best second generation prognostic tissue factors in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Basal as well as induced transcription from the human urokinase type plasminogen activator gene requires an enhancer containing two elements , a combined PEA3 / AP 1 and a consensus AP 1 site . ^^^ The COM region contains binding sites for four different unidentified urokinase type plasminogen activator enhancer factors ( UEF 1 to 4 ) , all four required for correct COM activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of urokinase type plasminogen activator in rat facial nucleus by axotomy of the facial nerve . ^^^ It is suggested that the urokinase type plasminogen activator might play a role in the events accompanying injury and regeneration in the facial nucleus following motoneuron lesion . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : Univariate analysis showed that a low tissue type plasminogen activator ( t PA ) activity in normal mucosa and in carcinomas and a high antigen level of inhibitor type 1 ( PAI 1 ) , and , to a lesser extent , of urokinase type plasminogen activator ( u PA ) receptor , in carcinomas are associated with a poor overall survival of the patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 2 ( PAI 2 ) in human keratinocytes regulates pericellular urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical localization of urokinase type plasminogen activator , type 1 plasminogen activator inhibitor , urokinase receptor and alpha ( 2 ) macroglobulin receptor in human breast carcinomas . ^^^ We have investigated the localization of urokinase type plasminogen activator ( u PA ) , type 1 plasminogen activator inhibitor ( PAI 1 ) , u PA receptor ( u PAR ) and alpha ( 2 ) macroglobulin receptor / low density lipoprotein receptor related protein ( alpha ( 2 ) MR / LRP ) in human breast tumors by immunohistochemical methods . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Exposure to EGF , dose dependently , led to an increased production of urokinase type plasminogen activator and M ( r ) 92 kD matrix metalloproteinase by the same cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Evidence for a novel binding protein to urokinase type plasminogen activator in platelet membranes . ^^^ Endogenous urokinase type plasminogen activator ( u PA ) has been identified in platelet membrane , and platelets have been shown to take up exogenous high molecular weight u PA from the ambient medium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Based on the staining results in xenografts and in human melanocytic lesions , we conclude that a strong correlation between expression of LRP and urokinase type plasminogen activator seems not to exist in in vivo melanomas . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We examined mRNA expressions of urokinase type plasminogen activator ( u PA ) , its specific receptor ( u PR ) , and plasminogen activator inhibitors ( PAI 1 and PAI 2 ) in 50 human breast cancers by the reverse transcriptase polymerase chain reaction method . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human plasminogen and urokinase type plasminogen activator bind to the surface of the spirochete where plasmin is generated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To further investigate possible involvement of the PA system , we quantified immunoreactive urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , both plasminogen activator inhibitors ( PAI 1 and PAI 2 ) and u PA receptor ( u PAR ) in synovial tissue extracts of 14 patients with rheumatoid arthritis ( RA ) and 12 with osteoarthritis ( OA ) . u PA , PAI 1 , PAI 2 and u PAR concentrations were significantly higher in RA than in OA patients . t PA antigen levels were significantly lower in RA than in OA synovial tissue extracts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Unexpectedly , changes in urokinase type plasminogen activator mRNA but not tissue type plasminogen activator mRNA correlated with fibrin deposition / dissolution in these tissues . ^^^ These results suggest that the absence of fibrin in some tissues reflects ongoing local fibrinolysis , and that increases in plasminogen activator inhibitory and tissue fac tor gene expression and decreases in urokinase type plasminogen activator expression are necessary for tissue specific fibrin deposition . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of single chain urokinase type plasminogen activator with a novel amino acid substitution in the kringle structure . ^^^ It was shown that ECV 304 secretes single chain urokinase type plasminogen activator ( scu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : In patients undergoing gastric surgery for malignant ( n = 18 ) or benign ( n = 21 ) disorders . , blood drawn from the portal vein and a peripheral vein was analysed for tissue type plasminogen activator antigen and activity ( tPA : Ag , tPA : Act ) , single chain urokinase type plasminogen activator activity ( scuPA : Act ) , and plasminogen activator inhibitor antigen and activity ( PAI : Ag , PAI : Act ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of a recombinant chimeric plasminogen activator composed of Gly Pro Arg Pro tetrapeptide and truncated urokinase type plasminogen activator expressed in Escherichia coli . ^^^ A chimeric plasminogen activator , GPRP u PA ( 144 411 ) , consisting of the Gly Pro Arg Pro tetrapeptide fused to the N terminal of a truncated urokinase type plasminogen activator ( comprising Leu 144 through Leu 411 ) , was produced by expression of the corresponding chimeric cDNA in Escherichia coli cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma samples for prothrombin fragment 1+2 , thrombin antithrombin 3 complex , plasmin alpha 2 antiplasmin complex , tissue type plasminogen activator antigen , urokinase type plasminogen activator antigen , u PA activity , plasminogen activity , alpha 2 antiplasmin and alpha 2 macroglobulin assays were obtained from AVF and contralateral large veins of ESRD patients and from peripheral veins of the control group . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Matrix proteases and the transcription factor c Ets 1 , which regulates in vitro stromelysin 1 , collagenase 1 , and urokinase type plasminogen activator gene promoters , are frequently expressed in invasive carcinomas . ^^^ Using in situ hybridization and immunohistochemistry , we analyzed collagenase 1 , stromelysins 1 and 3 , matrilysin , urokinase type plasminogen activator , and c Ets 1 gene expression on serial frozen sections of 39 intraepithelial bronchial lesions , including areas of hyperplasia , metaplasia , dysplasia , carcinoma in situ , and corresponding lung carcinomas in 13 patients . ^^^ Stromelysin 3 and urokinase type plasminogen activator were expressed only , but frequently , in preinvasive lesions ( dysplasia , carcinoma in situ ) and in carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A significant reduction in expression both of tissue type plasminogen activator and of urokinase type plasminogen activator occurred in PA 3 cells accompanying inhibition of IGF IR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor associates to a cell surface molecule in monocytic cells . ^^^ The monocyte like THP 1 cells express on their surface the urokinase type plasminogen activator receptor ( uPA R ) . ^^^ This molecule is probably linked to the cell surface by a glyco phospho inositol anchor since , by phospholipase C treatment , it is co eluted with the urokinase type plasminogen activator receptor from THP 1 cells . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( uPAR ) and integrins formed stable complexes that both inhibited native integrin adhesive function and promoted adhesion to vitronectin via a ligand binding site on uPAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Young and senescent cells were compared in quiescent and activated growth conditions for the secretion of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
IFN alpha significantly increased plasma levels of tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Similarly , a thrombolytic dose of urokinase type plasminogen activator ( 2 mg / kg , for 120 min , starting 15 min after thrombus induction ) caused a rapid and progressive resumption of blood flow ( up to 80 % of basal value ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Their enzymatic degradation by cultivated neurons , astrocytes , and microglia , as well as by purified urokinase type plasminogen activator , plasmin , thrombin , and trypsin , was investigated in an in vitro approach to elucidate the role of matrix degrading serine proteinases for inactivation of neuropeptides , especially those of higher amino acid chain length , in the brain . ^^^ The same cleavage pattern was observed , when neuropeptide Y and calcitonin gene related peptide were degraded by purified urokinase type plasminogen activator , plasmin , thrombin , and trypsin . ^^^ For further characterization of the neuropeptide degrading serine proteinase activities from cell cultures , urokinase type plasminogen activator was identified on microglia by immunostaining , whereas tissue type plasminogen activator mRNA occurred in neurons and astrocytes , but not in microglia . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increments in fibrinolytic parameters including tissue plasminogen activator antigen , urokinase type plasminogen activator antigen and activity , and plasminogen activity together with lower alpha 2 antiplasmin levels favor activation of fibrinolysis , except for higher alpha 2 macroglobulin concentrations , in arteriovenous fistula . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recently , generation of mice with single or combined deficiencies of main components of the fibrinolytic system , including plasminogen , tissue type and urokinase type plasminogen activator , plasminogen activator inhibitor 1 , and the cellular receptor for urokinase type plasminogen activator has allowed the role of the fibrinolytic system in vivo to be established more conclusively . ^^^ Tissue type plasminogen activator deficient mice have a reduced thrombolytic potential , whereas mice deficient in the urokinase type plasminogen activator occasionally develop spontaneous fibrin deposits in tissues and display deficient plasmin mediated macrophage function . ^^^ The urokinase type plasminogen activator / receptor system has been implicated in localized extracellular proteolytic activity . ^^^ Receptor deficient mice are , however , healthy , and fertile , and have a normal endogenous thrombolytic capacity ; their macrophages have , however , an impaired ( urokinase type plasminogen activator mediated ) plasminogen activating potential . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of urokinase type plasminogen activator expression by an ERK 1 dependent signaling pathway in a squamous cell carcinoma cell line . ^^^ The urokinase type plasminogen activator contributes to tissue remodeling by controlling the synthesis of the extracellular matrix degrading plasmin . ^^^ We undertook a study to determine the role of the extracellular signal regulated kinases ( ERKs ) in the regulation of urokinase type plasminogen activator expression in a squamous cell carcinoma cell line ( UM SCC 1 ) that contains a transcriptionally activated urokinase type plasminogen activator gene . ^^^ Transient transfection studies using a CAT reporter driven by the urokinase type plasminogen activator promoter , which had progressive 5 ' deletions or which had been point mutated , indicated the requirement of binding sites for AP 1 ( 1967 ) and PEA 3 ( 1973 ) for its maximal activation . ^^^ Expression of a mutant jun protein , which lacks the transactivation domain , caused a dose dependent repression of a CAT reporter driven by either the urokinase type plasminogen activator promoter or three tandem AP 1 repeats upstream of a thymidine kinase minimal promoter indicating the importance of AP 1 binding transcription factor ( s ) in the regulation of urokinase type plasminogen activator synthesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In gastric carcinomas the amount and activity of the tissue type plasminogen activator ( t PA ) have been reported to be decreased , whereas those of the urokinase type plasminogen activator ( u PA ) were increased , contributing to the neoplastic and invasive process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recombinant prourokinase ( r ProUK ) is a single chain urokinase type plasminogen activator that is produced from a mammalian cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Secretion of a variant of human single chain urokinase type plasminogen activator without an N glycosylation site in the methylotrophic yeast , Pichia pastoris and characterization of the secreted product . ^^^ Human single chain urokinase type plasminogen activator without an N glycosylation site ( scu PA Q 302 ) was produced in the methylotrophic yeast , Pichia pastoris using the shortened prepeptide sequence of a fungal aspartic proteinase , Mucor pusillus rennin ( MPR ) . ^^^ The level of urokinase type plasminogen activator ( u PA ) immunoreactive material in YPM medium was 0 . 47 mg / l ; however , most of the secreted product had been processed to smaller polypeptides . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombin and other mitogens regulate the expression of the urokinase type plasminogen activator receptor ( uPAR ) protein and mRNA levels in bovine vascular smooth muscle cells ( SMC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Minimally inflamed tissues did not react with antibody to urokinase type plasminogen activator , a potential activator of pro collagenase , but there was a consistent strong reaction in advanced disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Malignant tissue extracts had significantly higher levels of urokinase type plasminogen activator and plasminogen activator inhibitor 1 , but lower levels of tissue type plasminogen activator than normal mucosa . ^^^ Plasma levels of plasminogen activator inhibitor 1 antigen were significantly increased in cancer patients compared with controls , but there were no differences in tissue type and urokinase type plasminogen activator , in plasminogen activator inhibitor 2 , and D dimer levels . ^^^ Thus , the clinical relevance of plasma fibrinolytic parameters , especially urokinase type plasminogen activator antigen , as tumor markers in colorectal cancer remains to be established . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The recombinant bifunctional urokinase variant , M 23 ( rscu PA 40 kDA / Hir ) , comprising the kringle and protease domain of single chain urokinase type plasminogen activator and a C terminal fragment of hirudin in one single chain molecule , was evaluated for its thrombin inhibitory and fibrinolytic properties in vitro and in vivo . ^^^ In contrast , recombinant single chain urokinase type plasminogen activator ( saruplase ) inhibited neither blood coagulation nor platelet rich plasma aggregation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Apart from expression of urokinase type plasminogen activator , melanoma cells differ from cells derived from other tumors in the abundant expression of tissue type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Modulation of the receptor for urokinase type plasminogen activator in macrophage like U 937 cells by inflammatory mediators . ^^^ Macrophages in the tissues have been shown to express receptor for urokinase type plasminogen activator ( uPAR ) on their cell surface which plays an important role in cell invasion and attachment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Requirement of an upstream AP 1 motif for the constitutive and phorbol ester inducible expression of the urokinase type plasminogen activator receptor gene . ^^^ The urokinase type plasminogen activator receptor ( u PAR ) facilitates extracellular matrix proteolysis by accelerating plasmin formation at the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator induced monocyte adhesion is modulated by kininogen , kallikrein , factor 12 , and plasminogen . ^^^ Urokinase type plasminogen activator ( u PA ) was found to induce monocyte adhesion through a u PA receptor ( u PAR ) mediated cAMP dependent signal transduction pathway ( J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Antigen levels of tissue plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitors type 1 ( PAI 1 ) and PAI 2 in GCF were determined with ELISAs and 17 beta oestradiol and progesterone in serum with radioimmunoassays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effect of heat shock on the expression of urokinase type plasminogen activator receptor in human umbilical vein endothelial cells . ^^^ The level of urokinase type plasminogen activator ( u PA ) receptor ( u PAR ) mRNA increased up to 2 . 2 fold in response to heat shock , which was associated with the increased u PA binding and cell surface u PA activity determined by adding exogenous u PA to acid treated HUVECs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrin autography revealed that cultured bovine pulmonary artery endothelial cells spontaneously released tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) into the conditioned medium with no stimulus . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A sensitive bioimmunoassay for thrombin cleaved two chain urokinase type plasminogen activator in human body fluids . ^^^ Thrombin cleaves single chain urokinase type plasminogen activator ( scu PA ) into a two chain form ( tcu PA / T ) , which is virtually inactive in plasminogen activator assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study tissue plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and PAI 1 were localized in arterial biopsies of healthy and atherosclerotic vessels by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present report , we describe the thrombolytic and antithrombotic potencies of a hybrid recombinant plasminogen activator consisting of an antifibrin antibody 59D8 ( AFA ) and low molecular weight single chain urokinase type plasminogen activator ( scuPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Identification of urokinase type plasminogen activator receptor in human endothelial cells and its modulation by phorbol myristate acetate . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mRNAs for tissue inhibitor of metalloproteinase 1 and urokinase type plasminogen activator also showed a time dependent induction during the course of involution . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We examined the interference of endogenous inhibitors such as plasminogen activator inhibitor 1 ( PAI 1 ) and alpha 2 antiplasmin , and that of an endogenous activator namely single chain urokinase type plasminogen activator ( scu PA ) on the Coaset t PA assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Some of the enzymes which have been implicated in the spread of head and neck cancer include the urokinase type plasminogen activator and several members of the collagenase family such as type 1 and 4 collagenases and the stromelysins synthesized either by the tumor cells or in the surrounding fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of transforming growth factor beta 1 ( TGF beta 1 ) and urokinase type plasminogen activator ( u PA ) genes during arterial repair in the pig . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor associated urokinase type plasminogen activator : significance in breast cancer . ^^^ Urokinase type plasminogen activator ( u PA ) , a proteolitic enzyme , capable of degrading type 4 collagen , is supposed to be involved in degradation of extracellular matrix during cancer invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By immunocytochemical staining for the urokinase type plasminogen activator receptor ( uPAR ) which is a glycosyl phosphatidyl inositol ( GPI ) anchored protein expressed by normal , but not by PNH affected , neutrophils , it was shown that the uPAR positive subpopulation of normal neutrophils predominated among the faster migrating cells ( 60 80 % normal cells at the front of migration ) while uPAR negative ( i . e . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They bound melanoma t PA to a lower degree and did not bind urokinase type plasminogen activator at all , indicating specificity for t PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In patients , peritoneal fluid concentrations of tissue and urokinase type plasminogen activator were increased by factors of 65 and 10 respectively ( P < 0 . 05 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Domain domain interactions in hybrids of tissue type plasminogen activator and urokinase type plasminogen activator . ^^^ The plasminogen to plasmin conversion by urokinase type plasminogen activator ( u PA ) , in contrast to that of t PA , is not enhanced in the presence of fibrin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The increase seen for MCP 1 mRNA at 8 h was also observed for IL 8 mRNA but was not apparent for growth related gene expressions , urokinase type plasminogen activator ( u PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The antigen levels of plasminogen activator inhibitor type 1 ( PAI 1 ) , plasminogen activator inhibitor type 2 ( PAI 2 ) , tissue type plasminogen activator ( t PA ) , and urokinase type plasminogen activator ( u PA ) were measured during pregnancy and severe preeclampsia . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enhanced urokinase type plasminogen activator activity by extracellular matrix protein obtained from highly metastatic human lung adenocarcinoma cell line . ^^^ A protein which enhanced urokinase type plasminogen activator ( u PA ) activity was purified from the extracts of extracellular matrix of highly metastatic cell line HAL 8 derived from human lung adenocarcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The potential for hepatic metastasis in nude mice was studied by the intrasplenic implantation method with five human pancreatic cancer cell lines , Capan 1 , BxPC 3 , AsPC 1 , Panc 1 , and MIAPaCa 2 , especially in relation to serine protease expression , including urokinase type plasminogen activator and pancreatic trypsinogen 1 ( cationic form ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The two major fibrinolytic activators , urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) may play role in tumor spread and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Keratinocyte growth factor enhances urokinase type plasminogen activator activity in HPV 16 DNA immortalized human uterine exocervical epithelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator or pro urokinase is a zymogen with an intrinsic catalytic activity which is greater than that of most other zymogens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Secretory production of recombinant urokinase type plasminogen activator annexin 5 chimeras in Pichia pastoris . ^^^ To produce a thrombi targeting plasminogen activator , we expressed a fused gene that contains a modified pre sequence of Mucor pussilus rennin ( MPR ) followed by a chimeric gene of single chain urokinase type plasminogen activator ( scu PA ) : : annexin 5 ( AV ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of urokinase type plasminogen activator by fibroblast growth factor ( FGF ) 2 is dependent on expression of FGF receptors and does not require activation of phospholipase Cgamma 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the influence of increased liver blood flow on the pharmacokinetics and pharmacodynamics of recombinant tissue type plasminogen activator ( rt PA ) and to study the changes in endogenous urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PMA ( 10 nM ) induced a threefold increase in urokinase type plasminogen activator ( u PA ) mRNA , but u PA antigen levels in the HMC conditioned media remained below the detection level ( 0 . 5 ng / ml ) , possibly as a result of rapid uptake and degradation by the u PA receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Treatment of the endothelial cell monolayers with BFA resulted in an increase in the release of inhibitory material ( s ) against urokinase type plasminogen activator ( u PA ) activity of 3 LL cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The same concentrations of Dex led to a decrease in urokinase type plasminogen activator ( u PA ) synthesis and an increase in plasminogen activator inhibitor type 1 ( PAI 1 ) synthesis by SCC cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cell density dependent downregulation of urokinase type plasminogen activator in normal but not in transformed human epidermal keratinocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ets 1 regulates angiogenesis by inducing the expression of urokinase type plasminogen activator and matrix metalloproteinase 1 and the migration of vascular endothelial cells . ^^^ The expression of urokinase type plasminogen activator and matrix metalloproteinase 1 and the migration of ECs in response to growth factors were significantly inhibited by ets 1 antisense ODN but not by sense ODN . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The selected markers were growth regulators ( p 53 , Rb and myc ) , metastasis suppressor gene product ( nm 23 ) , adhesion molecules ( Ep CAM , E cadherin , CD44v5 and CD44v6 ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Renal biopsy tissue from 30 patients with MN along with specimens of normal kidney removed from six patients with renal tumors were examined for glomerular deposits of urokinase type plasminogen activator , tissue type plasminogen activator ( t PA ) , PAI 1 , VN , and fibrinogen by using immunofluorescence and immunoelectron microscopic techniques . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The elevated expression of the urokinase type plasminogen activator gene , which is necessary for the invasive phenotype of several types of cancers , is controlled by growth factors such as epidermal growth factor , transforming growth factor a , and fibroblast growth factor which bind to and activate protein tyrosine kinase transmembrane receptors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Analysis of cell conditioned medium by zymography and quantitative enzyme activity assays showed that clones transfected with a wild type CSF 1R expressed significantly higher levels of urokinase type plasminogen activator than did untransfected clones . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both cell lines expressed pro matrix metalloproteinase ( MMP ) 1 , proMMP 2 , proMMP 9 , membrane type MMP and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Finally , it was found that DC cultured cells produced lower levels of urokinase type plasminogen activator activity , while no differences were found in the metalloproteinase production compared to cells obtained from a tumor growing subcutaneously . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to combine plasminogen activating and thrombin inhibitory activities we constructed chimeric derivatives of recombinant single chain , urokinase type plasminogen activator ( rscu PA ) which comprise the kringle and protease domain of rscu PA fused via a linker sequence to a thrombin inhibitory domain . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin induced proteolysis of tenascin C : modulation by T lymphocyte derived urokinase type plasminogen activator and effect on T lymphocyte adhesion , activation , and cell clustering . ^^^ We demonstrate that stimulated T lymphocytes activate plasminogen by secreting the urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In a little group of persons because of small amounts of aqueous humor , other fibrinolytic parameters were also estimated and the presence of urokinase type plasminogen activator ( u PA ) , alfa 2 antiplasmin ( alfa 2AP ) and plasmin alfa 2 antiplasmin ( PAP ) complexes but no activity of protein C were observed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cysteine proteases ( cathepsin B and L ) , the serine protease urokinase type plasminogen activator and its inhibitor type 1 play an important part in cancer invasion and metastasis . ^^^ The Cox survival analysis showed that cathepsin B ( p = 0 . 002 ) , urokinase type plasminogen activator ( p = 0 . 0001 ) and the inhibitor type 1 ( p = 0 . 0004 ) significantly correlated with poor prognosis . ^^^ Urokinase type plasminogen activator was selected as the single independent variable in the Cox model ( p = 0 . 0001 ) . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibronectinase activity in cultured human trophoblasts is mediated by urokinase type plasminogen activator . ^^^ Cell mediated proteolytic activity could be partially inhibited by serum , whereas conditioned media containing fibronectinase activity was completely inhibited by serum , a serine protease inhibitor , and a selective inhibitor of urokinase type plasminogen activator . ^^^ CONCLUSIONS : Trophoblast derived urokinase type plasminogen activator has significant proteolytic activity in vitro with the capability of cleaving fibronectin into discrete fragments . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Treatment of human pleural mesothelioma ( MS 1 ) cells with phorbol myristate acetate ( PMA ) and cycloheximide results in 17 and 10 fold , respectively , increases in steady state expression of urokinase type plasminogen activator receptor ( uPAR ) mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Hemostatic changes after thrombolytic therapy with saruplase ( unglycosylated single chain urokinase type plasminogen activator ) and urokinase ( two chain urokinase type plasminogen activator ) . ^^^ Urokinase , or two chain urokinase type plasminogen activator ( tcu PA ) , is an effective thrombolytic agent . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) bound to its receptor , u PAR , initiates signal transduction pathways able to induce expression of the activator protein 1 ( AP 1 ) family member c fos [ 1 ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ratios of urokinase type plasminogen activator ( u PA ) to plasminogen activator inhibitor ( PAI l ) and matrix metalloprotease ( MMP ) 1 to tissue inhibitor of metalloprotease ( TIMP ) 1 were both markedly decreased . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MMP 9 ( gelatinase B ) and urokinase type plasminogen activator receptor ( u PAR ) , which are involved in cancer cell invasion and metastasis , are reported to be predominantly expressed by immune / inflammatory cells in human colorectal cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We compared globin mRNA , which is characteristically stable , and globin delta AU mRNA , which contains the 3 ' untranslated region of urokinase type plasminogen activator mRNA and is unstable . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Detailed analysis of the reaction products formed during the interaction between PAI 1 and its target proteinases tissue type plasminogen activator ( t PA ) or urokinase type plasminogen activator ( u PA ) , in the presence of these monoclonal antibodies , revealed two distinct mechanisms of PAI 1 inactivation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Detection of polymorphisms in the human urokinase type plasminogen activator gene . ^^^ The occurrence of published two amino acid sequences at position 194 of the human urokinase type plasminogen activator prompted us to search by SSCP for frequent polymorphisms in several exons of the gene . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Compared to the controls , plasma levels of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor ( PAI ) 1 antigen , D Dimer and enhanced thrombin generation were significantly ( P < 0 . 0001 ) increased in children with the common factor 5 mutation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both AGM 1470 and TEC 11 inhibited the production of urokinase type plasminogen activator ( u PA ) , an enzyme involved in the early steps of neovascularization . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Introduction of an RRHR motif into chicken urokinase type plasminogen activator ( ch uPA ) confers sensitivity to plasminogen activator inhibitor ( PAI ) 1 and PAI 2 and allows ch uPA mediated extracellular matrix degradation to be controlled by PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator receptor in hepatocellular carcinoma . ^^^ It is well known that a urokinase type plasminogen activator receptor ( uPAR ) is a key protein in the plasminogen activation system , which plays a proteolytically important role in the invasion and metastasis of various cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To give the donor cells a proliferative stimulus , a recombinant adenovirus vector that expresses a nonsecreted urokinase ( urokinase type plasminogen activator ) was transduced into the livers of recipient animals before transplantation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast , upregulation of urokinase type plasminogen activator expression was observed in bFGF treated microvascular MBECs and MHECs but not in MAECs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
On immunohistochemical studies , their specimens of prostatic carcinoma gave positive stain for PSA and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Relationship of urokinase type plasminogen activator , plasminogen activator inhibitor type 1 and activated protein C in fibrinolysis of human placenta . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Detection of heterologous Gs coupled receptor activity in LLC PK 1 cells based on expression of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interaction of urokinase type plasminogen activator with its receptor rapidly induces activation of glucose transporters . ^^^ The interaction of urokinase type plasminogen activator ( u PA ) or of u PA amino terminal fragment ( u PA ATF ) with the cell surface receptor ( u PAR ) was found to stimulate an increase of glucose uptake in many cell lines , ranging from normal and transformed human fibroblasts , mouse fibroblasts transfected with human u PAR , and cells of epidermal origin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The observed increase in urokinase type plasminogen activator ( u PA ) and its receptor ( u PAR ) in synovial tissue of patients with rheumatoid arthritis ( RA ) suggests pathophysiological involvement of the plasminogen activation ( PA ) system in inflammatory joint disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
During activation of the fibrinolytic system plasminogen is converted to plasmin by tissue plasminogen activator ( t PA ) or urokinase type plasminogen activator ( u PA ) . t PA is predominantly released from endothelial cells , u PA primarily by renal parenchymal cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Autocrine generated matrix metalloproteinases of tumor cells and the cascade network of protease activation mediated by membrane associated urokinase type plasminogen activator : involvement in the degradation of extracellular matrices ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of the low density lipoprotein receptor related protein ( LRP ) in the plasma clearance and liver uptake of recombinant single chain urokinase type plasminogen activator in rats . ^^^ Urokinase type plasminogen activator ( u PA ) is used as a thrombolytic agent in the treatment of acute myocardial infarction . ^^^ Treatment of isolated Kupffer cells with PI PLC reduced the binding of rscu PA by 40 % , suggesting the involvement of the urokinase type Plasminogen Activator Receptor ( u PAR ) in the recognition of rscu PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptors associate with beta 1 and beta 3 integrins of fibrosarcoma cells : dependence on extracellular matrix components . ^^^ We have shown previously that the urokinase type plasminogen activator receptor ( uPAR ) physically associates with beta 2 integrins on human leukocyte membranes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator , stromelysin 1 , stromelysin 3 , and matrilysin genes in lung carcinomas . ^^^ We have previously shown that the extracellular matrix degrading enzymes , urokinase type plasminogen activator ( u PA ) , stromelysin 1 , stromelysin 3 , and matrilysin , may play an important role in the transition from lung preneoplasia to invasive carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Wild type PAI 1 ( wtPAI 1 ) revealed the same conformational distribution pattern toward tissue type plasminogen activator ( t PA ) as toward urokinase type plasminogen activator ( u PA ) ( i . e . 53 + / 6 . 9 % active , 36 + / 6 . 8 % latent , and 12 + / 1 . 9 % substrate ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased expression of urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and collagenases in Caco 2 cells infected by Salmonella typhimurium . ^^^ Hypothesizing that the proteases might be provided by host cells , we investigated the changes of expression of urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and collagenases in epithelial cells ( Caco 2 ) infected with Salmonella typhimurium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Result showed decreased binding of plasminogen and the urokinase type plasminogen activator to the mesangial cell surface while the tissue type plasminogen activator and the plasminogen activator 1 associated with mesangial cells were increased . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Upregulation of urokinase type plasminogen activator by endogenous and exogenous HIV 1 Tat protein in tumour cell lines derived from BK virus / tat transgenic mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using this approach , the expression of RNAs for tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor 1 , plasminogen activator inhibitor 2 , protease nexin , and urokinase receptor isoform 1 ( uPAR 1 ) were detected in mouse osteoclasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : Significantly higher levels of cathepsins , urokinase type plasminogen activator , type 1 inhibitor , and significantly lower levels of tissue type plasminogen activator were found in active ulcers . ^^^ Bleeders had the highest cathepsins and urokinase type plasminogen activator levels . ^^^ Higher levels of urokinase type plasminogen activator , cathepsin B , and type 1 inhibitor were observed in Helicobacter pylori positive patients . ^^^ CONCLUSIONS : Our results suggest that impaired fibrinolysis ( tissue type plasminogen activator ) , intramucosal proteases ( cathepsins ) , tissue remodeling , and angiogenesis ( urokinase type plasminogen activator and type 1 inhibitor ) are involved in duodenal ulcer formation and healing . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Incubation with a two fold molar excess of ht PA ( n = 3 ) or human urokinase type plasminogen activator ( hu PA , n = 2 ) followed by analysis by SDS PAGE revealed reaction products corresponding to active ( 71 + / 7 % resp . 96 + / 3 . 6 % ) , latent ( 12 + / 0 . 4 % resp . 2 . 6 + / 2 . 4 % ) and substrate ( 16 . 6 + / 6 . 8 % resp . 1 . 5 + / 1 . 3 ) forms . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen dependent and independent proteolytic activity of marine endothelioma ( End ) cells that were derived from mice with targeted inactivation of the tissue type plasminogen activator ( t PA / ) , urokinase type plasminogen activator ( u PA / ) or plasminogen activator inhibitor 1 ( PAI 1 / ) genes was studied with the use of fibrin and extracellular matrix degradation assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , the ETS 1 antisense oligonucleotides reduced the levels of ETS 1 and urokinase type plasminogen activator mRNAs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Molecular modeling techniques combined with recently acquired biochemical / biophysical data were used to provide insight into the stable complex formation between plasminogen activator inhibitor 1 ( PAI 1 ) and the target proteinases : tissue type plasminogen activator , urokinase type plasminogen activator , and thrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we have significantly extended our earlier observations by identifying t PA variants that are substantially more resistant to inhibition by PAI 1 than any previously reported variants of t PA or urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Host urokinase type plasminogen activator participates in the release of malaria merozoites from infected erythrocytes . ^^^ Surface immunofluorescence and immunoprecipitation experiments , using specific polyclonal and monoclonal antibodies raised against human urokinase , demonstrate that this activity is due to the binding of host urokinase type plasminogen activator to the surface of erythrocytes infected by mature forms of Plasmodium falciparum malaria parasites . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cell surface urokinase type plasminogen activator receptor ( uPAR ) has been shown to be a key molecule in regulating plasminogen mediated extracellular proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Co expression of urokinase type plasminogen activator and its receptor in human gastric cancer cell lines correlates with their invasiveness and tumorigenicity . ^^^ The expression of urokinase type plasminogen activator ( u PA ) , its receptor ( u PAR ) and metalloproteases activity were analyzed in 4 human gastric cancer cell lines ( AGS , Hs746T , SNU 1 , and SNU 5 ) , in an attempt to relate these activities to their invasive potential and tumorigenicity on the modified chorioallantoic membranes ( CAM ) of chick embryos . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prostatic kallikrein hK 2 , but not prostate specific antigen ( hK 3 ) , activates single chain urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enzymes such as hyaluronidase , sialyltransferase , urokinase type plasminogen activator , plasmin , matrix metalloproteinases , and others , play central roles in the catabolism of extracellular matrix macromolecules . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
SF / HGF was found to stimulate ETS 1 mRNA and protein expressions , and these increases coincided with the dispersion of cells and the expression of protease mRNAs , such as urokinase type plasminogen activator and collagenase , but not with the protease inhibitor , plasminogen activator inhibitor type 1 . ^^^ Furthermore , we showed that SF / HGF was able to induce a transcriptional response involving ETS 1 by using artificial as well as cellular promoters , such as the urokinase type plasminogen activator and collagenase 1 promoters , containing RAS responsive elements with essential ETS binding sites . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen system , via its triggers , tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) , has been implicated in thrombosis , arterial neointima formation , and atherosclerosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Elevated urokinase type plasminogen activator receptor expression in a colon cancer cell line is due to a constitutively activated extracellular signal regulated kinase 1 dependent signaling cascade . ^^^ The urokinase type plasminogen activator receptor ( u PAR ) facilitates extracellular matrix degradation in part by accelerating plasmin formation at the cell surface . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of urokinase type plasminogen activator receptor by IL 1 beta . ^^^ We have previously reported that interleukin 1 beta ( IL 1 beta ) , an important inflammatory mediator in acute lung injury , upregulates urokinase type plasminogen activator expression by human A 549 cells ( 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have addressed this issue for two intimately related serine proteases , tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) , by modifying the technique of substrate phage display to create substrate subtraction libraries . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we investigated the presence of both LRP and urokinase type plasminogen activator receptor ( uPAR ) in astrocytoma tissues and in glioma cell lines by PCR and immunohistochemical analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator system in cancer metastasis : a review . ^^^ The urokinase type plasminogen activator ( u PA ) system consists of the serine proteinases plasmin and u PA ; the serpin inhibitors alpha 2 anti plasmin , PAI 1 and PAI 2 ; and the u PA receptor ( u PAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Physiological Plg activators are : tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our previous studies showed that glioblastomas express increased urokinase type plasminogen activator receptors ( uPARs ) in comparison to low grade gliomas ( Yamamoto et al . , Cancer Res . , 54 , 5016 5020 , 1994 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator in carcinomatous pleural fluid . ^^^ Urokinase type plasminogen activator ( u PA ) is known to be secreted by malignant cells during proliferation and migration , and is associated with tumour cell invasion and metastasis . ^^^ Our results suggest that local release of urokinase type plasminogen activator by viable cells , including cancer cells and mesothelial cells , may affect the levels of urokinase type plasminogen activator in pleural fluids . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Secretion of gelatinases ( probably MMP 2 / 72 kDa type i . v . collagenase ) and MMP 9 / 92 kDa type i . v . collagenase ) and urokinase type plasminogen activator were also investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The factors examined were cyclin D 1 , cyclin E , urokinase type plasminogen activator , 72 kd type 4 collagenase , vascular endothelial growth factor , platelet derived growth factor A ( PDGF A ) , transforming growth factor beta , and interleukin 10 . ^^^ Nodal metastasis correlated with cyclin D 1 ( p = 0 . 045 ) , cyclin E ( p = 0 . 037 ) , urokinase type plasminogen activator ( p = 0 . 047 ) , and PDGF A ( p = 0 . 003 ) mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We measured the antigen levels of urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in tissue extracts from nasopharyngeal carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human urokinase type plasminogen activator ( u PA ) is a member of the chymotrypsin family of serine proteases that can play important roles in both health and disease . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recently , a differential distribution of tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) has been demonstrated in bovine milk . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : Only the highly metastatic sublines AT 3 , MATLu , and MATLyLu showed a high extracellular matrix degradation mediated by urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The recombinant PSA failed to activate single chain urokinase type plasminogen activator , in contrast to the recombinant hK 2 , which readily activated single chain urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Significance in gene expression of matrix metalloproteinase 9 , urokinase type plasminogen activator and tissue inhibitor of metalloproteinase for metastases of gastric and / or colo rectal cancer ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Because of enhanced plasmin catalyzed proteolysis of plasminogen to des ( 1 77 ) plasminogen , activation by urokinase type plasminogen activator is also facilitated by denatured proteins ; activation of des ( 1 77 ) plasminogen is not affected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition of plasmin activity or inhibition of urokinase type plasminogen activator ( u PA ) activity resulted in approximately 40 % reduction of migration after 24 h in the wound assay and an even stronger reduction ( 70 80 % ) in the matrix invasion assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of urokinase type plasminogen activator in invasion of bile duct carcinoma ] . ^^^ OBJECTIVE : To study the role of urokinase type plasminogen activator ( u PA ) in the invasive process of bile duct carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase receptor ( uPAR ) binds urokinase type plasminogen activator ( u PA ) through specific interactions with uPAR domain 1 , and vitronectin through interactions with a site within uPAR domains 2 and 3 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present study was undertaken to evaluate in vitro the relative importance of tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) in the mitogenic and chemotactic potential of bovine fibroblast growth factor ( bFGF ) and platelet derived growth factor ( PDGF ) BB for smooth muscle cells ( SMC ) . ^^^ Aortic SMC were isolated from transgenic mice showing single inactivations of the t PA , u PA , plasminogen activator inhibitor 1 , or urokinase type plasminogen activator receptor ( u PAR ) genes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Identification of a flexible loop region ( 297 313 ) of urokinase type plasminogen activator , which helps determine its catalytic activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
E cadherin ) and to matrix components ( e . g . integrins , hyaluronic acid receptors ) ; ( 4 ) factors which regulate detachment from the extracellular matrix ( e . g . urokinase type plasminogen activator , matrix metalloproteinases ) ; and ( 5 ) molecules which regulate cytoskeletal function ( e . g . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , the anti apoptotic activity of TGFbeta on cultured vascular smooth muscle cells ( SMC ) isolated from the aorta of transgenic mice with single inactivation of genes encoding the tissue type plasminogen activator ( t PA ( / ) ) , urokinase type plasminogen activator ( u PA ( / ) ) , urokinase receptor ( u PAR ( / ) ) or plasminogen ( Plg ( / ) ) genes was examined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The recombinant unglycosylated single chain urokinase type plasminogen activator saruplase is cleared for a large part by the liver . ^^^ Blood samples were taken at regular time intervals to measure plasma levels of urokinase type plasminogen activator ( u PA ) antigen and activity , the two chain form ( tcu PA ) activity , indocyanine green , fibrinogen , fibrin and fibrin degradation products , alpha 2 antiplasmin and thrombin antithrombin 3 complex . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In human colon carcinomas , urokinase type plasminogen activator ( u PA ) produced by stroma cells can bind to cancer cell associated u PA receptor ( u PAR ) , and then catalyze the conversion of plasminogen ( Pg ) into plasmin ( Pm ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary physiological inhibitor of both tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : We examined the patterns of expression of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and vitronectin in head and neck squamous cell carcinomas using immunohistochemical techniques . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previous studies have shown that activity of urokinase type plasminogen activator ( u PA ) increases very rapidly ( within 1 minute ) after partial hepatectomy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombin stimulation of platelets induces plasminogen activation mediated by endogenous urokinase type plasminogen activator . ^^^ Gene knockout mice studies indicate that urokinase type plasminogen activator ( u PA ) is importantly involved in fibrinolysis , but its physiologic mechanism of action remains poorly understood . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
All subjects achieved successful reperfusion [ thrombolysis in myocardial infarction ( TIMI ) flow grade 3 ] through intracoronary thrombolysis with single chain urokinase type plasminogen activator ( SCU PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Efficient production of a functional mouse / human chimeric Fab ' against human urokinase type plasminogen activator by Bacillus brevis . ^^^ For the production of Fab ' , the cDNAs encoding the L chain and Fd ' fragment ( Fd with the hinge region ) of a mouse human chimeric Fab ' against human urokinase type plasminogen activator were fused directly with the translation start and signal peptide encoding regions of the mwp gene , the gene for one of the major cell wall proteins of Bacillus brevis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A prospective study on the prognostic significance of urokinase type plasminogen activator levels in breast cancer tissue . ^^^ Urokinase type plasminogen activator ( u PA ) , which cleaves plasminogen to yield plasmin , is a serine protease of fibrinolysis and is presumed to play a key role in extracellular proteolysis and facilitate the migration of cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Staphylococcus aureus stimulates urokinase type plasminogen activator expression by bovine mammary cells . ^^^ By use of bovine mammary epithelial and myoepithelial cell lines , it was found that bovine S . aureus M 60 and its culture filtrates induce a 3 to 10 fold increase in urokinase type plasminogen activator activity in mammary cell conditioned media and cellular lysates . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin , generated from the zymogen plasminogen by tissue type plasminogen activator ( t PA ) or urokinase type plasminogen activator ( u PA ; refs 14 , 15 ) , has been proposed as a possible activator in vitro , but evidence for such a role in vivo is lacking . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased focal adhesion kinase and urokinase type plasminogen activator receptor associated cell signaling in endothelial cells exposed to asbestos . ^^^ Affinity purification of GPI anchored urokinase type plasminogen activator receptor ( uPAR ) from chrysotile exposed cells demonstrated that asbestos altered the profile of proteins and phosphoproteins complexed with this receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Organ site dependence for the production of urokinase type plasminogen activator and metastasis by human renal cell carcinoma cells . ^^^ We examined the role of urokinase type plasminogen activator ( u PA ) in the metastasis of the human renal cell carcinoma ( HRCC ) implanted in athymic nude mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Only the full agonist mAb is able to induce and sustain the expression of urokinase type plasminogen activator receptor for prolonged periods of time , while both mAbs up regulate the constitutive expression of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
However , unlike other known plasminogen activator inhibitors , neuroserpin is a more effective inactivator of tPA than of urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Components of the coagulation ( thrombin antithrombin complexes , prothrombin fragment F 1 + 2 , activated factor 7 , and factor 7 antigen ) and fibrinolytic ( fibrin split product D dimer , plasmin plasmin inhibitor complex , tissue type plasminogen activator , urokinase type plasminogen activator , and plasminogen activator inhibitor 1 ) systems and markers of endothelial cell perturbation / dysfunction ( von Willebrand factor and thrombomodulin ) were measured before the start of infusion and after 2 , 6 , 12 , and 24 hours . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We here demonstrate by immunofluorescence that TFPI colocalizes in EC with caveolin , urokinase type plasminogen activator receptor , and glycosphingolipids . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect of TNP 470 on cell proliferation and urokinase type plasminogen activator and its inhibitor in human lung cancer cell lines . ^^^ We therefore investigated the inhibitory effect of TNP 470 on cancer cell proliferation , and the suppressive effect of TNP 470 on urokinase type plasminogen activator ( u PA ) and its inhibitor ( PAI 1 ) , in human lung cancer cell lines in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Receptor independent role of urokinase type plasminogen activator in pericellular plasmin and matrix metalloproteinase proteolysis during vascular wound healing in mice . ^^^ It has been proposed that the urokinase receptor ( u PAR ) is essential for the various biological roles of urokinase type plasminogen activator ( u PA ) in vivo , and that smooth muscle cells require u PA for migration during arterial neointima formation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An efficient system for production of recombinant urokinase type plasminogen activator . ^^^ A system was developed to produce recombinant urokinase type plasminogen activator in Escherichia coli . ^^^ The urokinase type plasminogen activator was produced with a 6 10 His tag at the C terminus which was shown to have the same activity , after refolding , as the wild type protein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fourth , 38 kDa C terminal truncated rMaspin ( 1 ) further stimulates fibrinogen / gelatin associated sctPA . rMaspin ( 1 ) acts specifically ; it does not inhibit urokinase type plasminogen activator , plasmin , chymotrypsin , trypsin , or elastase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Based on the full length cDNAs obtained from RACE , these mRNAs presumably encode an 88 amino acid protein ( DRTP 1 , differentially regulated trout protein 1 ) that is homologous to a gene superfamily composed of snake venom neurotoxins , a CD 59 complement regulatory protein , Ly 6 alloantigens , and a urokinase type plasminogen activator receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The influence of loop size and target sequence on selective proteolysis by tissue type plasminogen activator and urokinase type plasminogen activator . ^^^ We have previously used substrate phage display to identify peptide sequences that are efficiently and selectively cleaved by tissue type plasminogen activator ( t PA ) or urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of urokinase type plasminogen activator is a novel prognostic factor in dukes B and C colorectal cancer . ^^^ Urokinase type plasminogen activator ( u PA ) secreted by cancer cells is considered to play a key role in promoting invasion and metastasis of cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The tested hemostatic parameters were urokinase type plasminogen activator , tissue type plasminogen activator and plasminogen activator inhibitor as fibrinolytic parameters and von Willbrand factor , tissue factor , antithrombin 3 , factor 10 and factor Xa as coagulation parameters . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cellular localization of urokinase type plasminogen activator , its inhibitors , and their mRNAs in breast cancer tissues . ^^^ Urokinase type plasminogen activator ( u PA ) mRNA was detected in cancer cells and fibroblasts adjacent to them and its expression was found to be more intense in invasive than in intraductal regions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zymography for proteolytic enzymes demonstrated high levels of type 4 collagenase and urokinase type plasminogen activator ( u PA ) activity in GB d 1 , GB h 3 and GB d 2 even in the absence of HGF . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We found that inhibition of p 38 by SB 203580 resulted in the almost complete reduction of phorbol myristate acetate induced MMP 9 secretion but not of urokinase type plasminogen activator secretion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prognostic impact of urokinase type plasminogen activator ( PA ) , PA inhibitor type 1 , and tissue type PA antigen levels in node negative breast cancer : a prospective study on multicenter basis . ^^^ Urokinase type plasminogen activator ( u PA ) is a key protease in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The relative distribution of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and plasminogen activator inhibitor 2 ( PAI 2 ) was studied in cultured human gingival fibroblasts , healthy gingival tissues and inflamed gingival tissues by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator is implicated in cell invasion and migration . ^^^ In this study , we provide evidence for the presence of urokinase type plasminogen activator ( 3 . 5 + / 1 . 5 ng / mg of cell protein ) in isolated peripheral blood eosinophils , in comparison to 6 . 7 + / 2 . 3 ng / mg of protein in neutrophils . ^^^ Both immunocytochemical and immunogold staining of urokinase type plasminogen activator on electron microscopy showed its localization in granules of resting cells . ^^^ Mild stimulation by platelet activating factor induced a translocation of urokinase type plasminogen activator from the granules to the cell membrane and a formation of urokinase type plasminogen activator capping , suggesting a role for urokinase type plasminogen activator in eosinophil migration . ^^^ The activation of eosinophils led to the release of factor ( s ) which , after activation of pro urokinase type plasminogen activator , induced an inactivation of generated urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transcriptional regulation of urokinase type plasminogen activator receptor by cyclic AMP in PL 21 human myeloid leukemia cells : comparison with the regulation by phorbol myristate acetate . ^^^ We investigated the effect of dibutyryl cyclic AMP ( Bt 2 cAMP ) on urokinase type plasminogen activator receptor ( uPAR ) expression in human PL 21 myeloid leukemia cells and compared it with the effect of phorbol myristate acetate ( PMA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Human RPE cells were transduced with retroviral vectors bearing either a urokinase type plasminogen activator ( u PA ) or a tissue type plasminogen activator ( t PA ) cDNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : We examine here the prognostic significance of cyclin D 1 , urokinase type plasminogen activator , vascular endothelial growth factor ( VEGF ) , platelet derived growth factor , and c erbB 2 expression in pre operatively obtained fine needle aspirates from breast carcinomas less than or equal to 3 cm in size . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Because earlier studies have shown that the receptor for urokinase type plasminogen activator ( uPAR ) may facilitate such events , we studied the effect of hypoxia on the expression of uPAR by first trimester human trophoblasts ( HTR 8 / SVneo ) and human umbilical vein endothelial cells ( HUVEC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( uPAR ) plays an important role on the cell surface in mediating extracellular degradative processes and formation of active TGF beta , and in nonproteolytic events such as cell adhesion , migration , and transmembrane signaling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By using specific inhibitors and different anti plasminogen activators antibodies it is shown that only one type of the enzyme , the urokinase type plasminogen activator , is expressed during the cerebellum ontogeny . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The level of urokinase type plasminogen activator receptor is increased in serum of ovarian cancer patients . ^^^ Ascites and serum of patients with ovarian carcinoma contain a soluble form of urokinase type plasminogen activator receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Involvement of vascular endothelial growth factor and urokinase type plasminogen activator receptor in microvessel invasion in human colorectal cancers . ^^^ To evaluate the association among known angiogenic growth factors or factors related to the plasminogen activation system and clinicopathological factors in patients with colorectal cancer , we examined the expression of vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , transforming growth factor alpha ( TGF alpha ) , urokinase type plasminogen activator ( u PA ) , u PA receptor ( u PA R ) and plasminogen activator inhibitor 1 ( PAI 1 ) in clinical specimens of colorectal cancers by Northern blot analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proteolytic cleavage of urokinase type plasminogen activator by stromelysin 1 ( MMP 3 ) . ^^^ Matrix metalloproteinase 3 ( MMP 3 , or stromelysin 1 ) specifically hydrolyzes the Glu 143 Leu144 peptide bond in 45 kDa single chain urokinase type plasminogen activator ( scu PA ) and in its two chain ( tcu PA ) derivative , yielding a 17 kDa NH 2 terminal domain comprising the u PA receptor ( u PAR ) binding site and a 32 kDa COOH terminal moiety containing the serine proteinase domain of u PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Data from past studies of colon carcinoma indicate that the tissue expression of matrix metalloproteinase 9 and urokinase type plasminogen activator receptor is inversely associated with simultaneous liver metastasis and infiltrating growth pattern . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To assess this hypothesis , Namalwa , Raji , and Daudi cell lines ( Burkitt ' s lymphoma ) , LIK and SB cell lines ( B cell lymphoblastic leukemia ) , CEM and Jurkat cell lines ( T cell lymphoblastic leukemia ) , and U 266 cell line ( multiple myeloma ) were evaluated for their capacity to produce matrix metalloproteinase 2 and 9 , and urokinase type plasminogen activator . ^^^ In contrast , urokinase type plasminogen activator was secreted only by SB cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator ( u PA ) , u PA receptor , and tissue type PA messenger RNAs in human hepatocellular carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serine proteinase inhibitor ( serpin ) plasminogen activator inhibitor type 2 ( PAI 2 ) is well characterized as an inhibitor of extracellular urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The relation between the growth patterns of gastric carcinoma and the expression of hepatocyte growth factor receptor ( c met ) , autocrine motility factor receptor , and urokinase type plasminogen activator receptor . ^^^ BACKGROUND : Hepatocyte growth factor receptor ( c met ) , autocrine motility factor receptor ( AMFR ) , and urokinase type plasminogen activator receptor ( uPAR ) are known to play important roles in tumor cell migration , invasion , and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Correlates of urokinase type plasminogen activator in colorectal cancer : positive relationship with nm 23 and c erbB 2 protein expression . ^^^ We undertook a study to analyze the expression of urokinase type plasminogen activator ( u PA ) protein in colorectal cancer ( CRC ) and to compare it with c erbB 2 ( HER2 / neu ) and nm 23 protein expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Vascular endothelial growth factor expression is reduced in liver metastasis from colorectal cancer and correlates with urokinase type plasminogen activator . ^^^ We undertook a retrospective study to investigate the correlation between the expression of vascular endothelial growth factor ( VEGF ) and urokinase type plasminogen activator ( u PA ) proteins with progression of colorectal carcinoma ( CRC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We compared the fibrinolytic activities of IVEC and HUVEC , and observed that IVEC express a more profibrinolytic phenotype than HUVEC , since they bind and activate plasminogen more efficiently , produce more tissue plasminogen activator and urokinase type plasminogen activator antigens , and secrete less plasminogen activator inhibitor 1 antigen both under basal conditions and after stimulation with lipopolysaccharide , phorbol ester and tumor necrosis factor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Testicular LHRH peptidase and testicular urokinase type plasminogen activator are Sertoli cell secreted proteases which display similar molecular properties . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cell cycle dependent fluctuation of urokinase type plasminogen activator , its receptor , and inhibitors in cultured bovine mammary epithelial and myoepithelial cells . ^^^ Bovine mammary epithelial ( BME UV 1 , clone E T and BME UV , clone E T 2 ) and myoepithelial ( BMM UV , clone m T 2 ) cell lines were used to study the modulation of cell associated activity of urokinase type plasminogen activator ( u PA ) , as well as mRNA transcripts of u PA , its receptor ( u PAR ) , and inhibitors ( PAI 1 and PAI 2 ) during the cell cycle . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Samples acquired by directional coronary atherectomy from 25 patients with type 2 diabetes and 18 patients without diabetes were characterized qualitatively histologically for cellularity and by immunohistochemistry visually and qualitatively and by quantitative image analysis for assessment of urokinase type plasminogen activator ( u PA ) and PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Overexpression of urokinase type plasminogen activator and its receptor correlates with metastatic capacity in breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Size exclusion HPLC and rates of plasminogen activation by urokinase type plasminogen activator were used to determine the conformational states of these variants . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Suppression of type 1 collagenase expression by antisense RNA in melanoma cells results in reduced synthesis of the urokinase type plasminogen activator receptor . ^^^ This increased extracellular plasmin activity coincided with reduced membrane associated plasminogen levels and decreased expression of the urokinase type plasminogen activator receptor at both the mRNA ( up to 83 % reduction ) and cell surface ( up to 48 % reduction ) levels , while urokinase mRNA levels remained unchanged . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This compound was a rapid inactivator of urokinase type plasminogen activator . ^^^ These results suggest the involvement of a serine proteinase ( s ) with trypsin like specificity , possibly urokinase type plasminogen activator , in chondrocyte mediated cartilage proteoglycan breakdown occurring as a result of stimulation with proinflammatory cytokines . ^^^ It remains to be shown whether the target serine proteinase is urokinase type plasminogen activator . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using chorio allantoic membranes ( CAMs ) of chick embryos and severe combined immunodeficient ( SCID ) mice , we investigated the effects of urokinase type plasminogen activator receptor ( u PAR ) over expression on the process of invasion and tumorigenicity . ^^^ By the transfection of u PAR cDNA , 3 u PAR over expressing clones expressing 1 . 6 to 4 . 6 fold more u PAR mRNA than parent cells were obtained from a human epidermoid carcinoma cell line , HEp 3 , that expresses urokinase type plasminogen activator ( u PA ) and u PAR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Bronchoalveolar fibrinolytic activity , due to urokinase type plasminogen activator ( u PA ) , was significantly depressed after endotoxin injection , mainly due to a striking increase in plasminogen activator inhibitor 2 levels in BAL fluid . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate a potential physiological role of the plasminogen / plasmin system in activation of the matrix metalloproteinase ( MMP ) system , the distribution of latent and active MMP 2 ( gelatinase A ) or MMP 9 ( gelatinase B ) was monitored in aorta extracts and in serum free conditioned cell culture medium obtained from wild type ( WT ) mice and from mice with deficiency of tissue type plasminogen activator ( t PA ( / ) ) , urokinase type plasminogen activator ( u PA ( / ) ) , plasminogen activator inhibitor 1 ( PAI 1 ( / ) ) or plasminogen ( Plg ( / ) ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Endothelial cells ( ECs ) play a pivotal role in maintaining normal hemostasis by regulating fibrinolysis through the synthesis of plasminogen activators ( PAs ) , tissue type plasminogen activator ( t PA ) , and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A previous study has reported a high frequency of rare alleles of the urokinase type plasminogen activator receptor ( uPAR ) in a set of 15 colorectal cancer ( CRC ) cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Role of tissue specific transcription factor LFB 3 in a cyclic AMP responsive enhancer of the urokinase type plasminogen activator gene in LLC PK 1 cells . ^^^ A cyclic AMP ( cAMP ) inducible enhancer in the pig urokinase type plasminogen activator gene located 3 . 4 kb upstream of the transcription initiation site is composed of three protein binding domains , A , B , and C . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stimulation of urokinase type plasminogen activator receptor expression by PMA requires JNK 1 dependent and independent signaling modules . ^^^ The urokinase type plasminogen activator receptor ( u PAR ) has been implicated in tumor progression , and previous studies have shown that the expression of this gene is strongly up regulated by PMA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Leukaemic and normal bone marrow samples were compared in terms of their content of the fibrinolytic agents , tissue plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) and their inhibitors . plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effects of testosterone , dexamethasone , at RA , and 9 cis RA on tube formation were accompanied by parallel changes in urokinase type plasminogen activator ( u PA ) expression , at both mRNA and antigen levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The inactivation of single chain urokinase type plasminogen activator by thrombin may provide an additional explanation for the antifibrinolytic effect of factor 11 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using equilibrium binding studies with radiolabeled 125I labeled single chain urokinase type plasminogen activator ( scu PA ) , we determined that SMC cultured from atherosclerotic arteries expressed a higher maximal number of binding sites / cell ( 3 . 6 + / 0 . 4 10 10 ( 5 ) sites / cell vs . 2 . 1 + / 0 . 3 10 10 ( 5 ) , + / SEM , p < 0 . 05 ) with a similar affinity ( Kd = 1 . 5 + / 0 . 1 vs . 1 . 2 + / 0 . 2 nM , p = ns ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The production of urokinase type plasminogen activator ( u PA ) by synovial cells and chondrocytes and the subsequent interaction of u PA with its membrane receptor ( u PAR ) is under the control of a variety of growth factors and cytokines released within the inflamed joints . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Production of urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) in human brain tumours . ^^^ The amounts of urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor 1 ( PAI 1 ) , and the activity of u PA and t PA were determined by enzyme linked immunosorbent assay ( ELISA ) , and u PA and PAI 1 were immunolocalized using monoclonal antibodies in human brain tumours and normal brain tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , we determined the plasma and tissue concentrations of tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor 1 , plasminogen activator inhibitor 2 and urokinase type plasminogen activator receptor in 32 patients with pathology proved gastric cancer . ^^^ Plasma plasminogen activator inhibitor 1 was significantly higher in gastric cancer than in benign gastric disease ( p < 0 . 0005 ) , whereas plasma urokinase type plasminogen activator was significantly lower in patients with gastric cancer than in those with benign ulcer ( p = 0 . 003 ) . ^^^ The plasma and tissue levels of fibrinolytic parameters were not affected by tumor size or distant metastasis , whereas tumor tissue concentration of urokinase type plasminogen activator receptor and plasminogen activator inhibitor 2 were significantly higher in N 0 than in N 1 and N 2 , and tissue plasminogen activator inhibitor 1 was significantly higher in N 0 than in N 1 . ^^^ Tissue concentrations of urokinase type plasminogen activator receptor and plasminogen activator inhibitor 2 , especially the latter , can be used to predict lymph node involvement in patients with gastric cancer . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The inactivation of single chain urokinase type plasminogen activator by thrombin in a plasma milieu : effect of thrombomodulin . ^^^ Thrombin cleaves single chain urokinase type plasminogen activator ( scu PA ) into a virtually inactive two chain form ( tcu PA / T ) , a process which may contribute to the maintenance of a fresh blood clot . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since urokinase type plasminogen activator and plasmin are known to play significant roles in tumour cell metastasis , the dose dependent inhibitory effect of FBPI with antiplasmin activity on tumour cell metastasis suggests that its antimetastatogenic action is probably mediated through its plasmin inhibitory action . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The transmembranous urokinase type plasminogen activator receptor ( uPAR ; CD 87 ) focuses the formation of active plasmin at the cell surface , thus enhancing directional extracellular proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although thrombin and urokinase type plasminogen activator were not detected in the primary screen , low level expression was confirmed using specific polymerase chain reaction primers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of urokinase type plasminogen activator ( u PA ) , u PA receptor ( u PAR ) and plasminogen activator inhibitor ( PAI ) 1 and 2 was examined in 105 cases of primary lung cancer tissue using immunohistochemical staining and reverse transcriptase polymerase chain reaction ( RT PCR ) techniques . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To evaluate a gene complement leading to matrix metalloproteinase 2 ( MMP 2 ; Mr 72 , 000 gelatinase ) activity , membrane type 1 MMP ( MT 1 MMP ) , urokinase type plasminogen activator , MMP 2 , and tissue inhibitor of metalloproteinase 2 transcriptional levels were measured in gastric carcinoma biopsies . ^^^ Instead , the expression of urokinase type plasminogen activator , which trips off a cascade ending in the activation of MMP 2 , as well as the expression of MMP 2 itself and its inhibitor , tissue inhibitor of metalloproteinase 2 , lacked correlation with patient follow up . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding of VEGF B to its receptor on endothelial cells leads to increased expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1 , suggesting a role for VEGF B in the regulation of extracellular matrix degradation , cell adhesion , and migration . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , we demonstrated that the urokinase type plasminogen activator ( u PA ) induced neovascular growth in the avascular rabbit cornea and dose dependently promoted growth , chemotaxis , and matrix invasion of cultured endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The results suggest that ets 1 , probably through transactivation of genes such as urokinase type plasminogen activator and matrix metalloproteinases , might play a crucial role in the differentiation of the cushion and subepicardial mesenchyme , the formation of the intratrabecular sinusoids and the early development of the cardiac vessels . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We recently reported that in vivo phosphorylation of urokinase type plasminogen activator on Ser138 / 303 prevents its catalytic independent ability to promote myelomonocytic cell adherence and motility . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The tissue concentrations of urokinase type plasminogen activator ( u PA ) , urokinase type plasminogen activator receptor ( u PAR ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) were investigated by an ELISA technique in normal and malignant samples of the prostate from 24 patients undergoing radical prostatectomy for organ confined prostate cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : We studied seven variables of the coagulation pathway ( PT , aPTT , fibrinogen , FVIII : c , von Willebrand factor , thrombin antithrombin complexes , prothrombin fragments 1+2 ) and seven parameters of the fibrinolytic system ( plasminogen , alpha 2 antiplasmin , C 1 esterase inhibitor , tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor type 1 , D dimer ) in 24 pediatric patients with diarrhea associated hemolytic uremic syndrome and in 15 children with acute renal failure not due to hemolytic uremic syndrome . ^^^ In contrast , plasma levels of tissue type plasminogen activator and urokinase type plasminogen activator are significantly higher in the hemolytic uremic patients than in those with acute renal failure of other causes ( P < 0 . 01 and P < 0 . 05 respectively ) . ( 4 ) Hemodialysis leads to an increase in tissue type plasminogen activator antigen and a decrease of plasminogen activator inhibitor 1 activity levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A 150 156 amino acids deleted single chain urokinase type plasminogen activator ( dscu PA ) and its recombinant wild type counterpart ( rscu PA ) were both expressed in Escherichia coli . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
All of the neurotrophins tested nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , neurotrophin 3 ( NT 3 ) , and neurotrophin 4 ( NT 4 ) , increased the secretion of plasminogen and urokinase type plasminogen activator and specific activity of acid phosphatase , but suppressed the release of constitutively produced and lipopolysaccharide stimulated nitric oxide ( NO ) from microglia . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression and functional role of urokinase type plasminogen activator receptor in normal and acute leukaemic cells . ^^^ Urokinase type plasminogen activator receptor ( UPA R CD 87 ) is a GPI anchored membrane protein which promotes the generation of plasmin on the surface of many cell types , probably facilitating cellular extravasation and tissue invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of our study was to measure fibrinogen and fibrin degradation products ( D dimer ) , interleukin 6 tissue plasminogen activator , plasminogen activator inhibitor 1 and urokinase type plasminogen activator in the plasma and arterial walls of 45 patients who had arterial surgery between April 1993 and November 1995 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator in colorectal cancer : relationship with clinicopathological features and patient outcome . ^^^ Urokinase type plasminogen activator ( u PA ) is a serine protease that has been implicated in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Retinoic acid modulates extracellular urokinase type plasminogen activator activity in DU 145 human prostatic carcinoma cells . ^^^ Effects of all trans retinoic acid ( RA ) on the net enzymatic activity of secreted , extracellular urokinase type plasminogen activator ( u PA ) in DU 145 human prostatic carcinoma cells were examined to assess the potential use of retinoids in human prostate cancer prevention and treatment . u PA is associated with tumor progression involving invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both normal and malignant prostatic epithelial cells in culture secrete urokinase type plasminogen activator ( u PA ) into the culture medium . u PA has been shown to have a direct association with invasive and metastatic potential of many types of cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVES : To establish an orthotopic murine floor of mouth cancer model for the analysis of the role of proteases such as urokinase type plasminogen activator ( u PA ) and the matrix metalloprotease MMP 9 ( MMP 9 ) in in vivo invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Treatment of LRP expressing cell lines with the LRP ligands lactoferrin or urokinase type plasminogen activator caused a significant elevation in cAMP and stimulated PKA activity in a dose dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor expression in colorectal neoplasms . ^^^ BACKGROUND : The urokinase type plasminogen activator receptor ( uPAR ) may play a critical role in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) specific staining was observed in pseudostratified ciliated epithelium and was predominant in mucous cells of the seromucinous gland , while serous cells were almost devoid of stain . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This protein may prove to be a new member of the family of integrin associated , GPI anchored proteins , which includes urokinase type plasminogen activator receptor ( uPAR ) , lipopolysaccharide ( LPS ) / LPS binding protein ( LBP ) receptor ( CD 14 ) , and Fcgamma receptor IIIB ( CD16b ) ; all of which are regulators of integrin function . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The structural and functional properties of the urokinase type plasminogen activator ( u PA ) that are involved in the mitogenic effect of this proteolytic enzyme on human melanoma cells M 14 and IF 6 and the role of the u PA receptor ( u PAR ) in transducing this signal were analyzed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , zymographic analysis revealed that noncytotoxic concentrations ( < 10 microM ) of TAC 101 inhibited TPA induced production of urokinase type plasminogen activator ( u PA ) and matrix metalloproteinase ( MMP ) 9 from tumor cells , which is considered to be associated with their invasive and metastatic potentials . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of urokinase type plasminogen activator and plasminogen activator inhibitor type 1 in particular have been reported to be of clinical and prognostic value . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression and functional role of urokinase type plasminogen activator receptor ( UPA R ; CD 87 ) in normal and acute leukemia cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Evidence for the expression of urokinase type plasminogen activator by human venous endothelial cells in vivo . ^^^ Endothelial cells ( ECs ) in culture synthesize and secrete urokinase type plasminogen activator ( u PA ) , but the normal vascular endothelium is believed to synthesize only tissue plasminogen activator ( t PA ) , which is thought to be responsible for intravascular fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stromal expression of urokinase type plasminogen activator receptor ( uPAR ) is associated with invasive growth in primary liver cancer . ^^^ AIMS / BACKGROUND : Expression of urokinase type plasminogen activator receptor ( uPAR ) was studied in 25 hepatocellular carcinomas ( HCCs ) and seven cholangiocellular carcinomas ( CCCs ) by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BCL 2 expressing cells showed an increase in MMP 2 / 3 / 12 ( LN 18 ) , and MMP 9 / 12 and cell surface urokinase type plasminogen activator ( u PA ) ( LN 229 ) mRNA and a reduction in tissue inhibitors of metalloproteinases ( TIMP ) 2 mRNA ( LN 229 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
F 3 fraction enhanced mRNA expression of urokinase type plasminogen activator ( u PA ) , matrix metalloproteinase 2 ( MMP 2 ) , and membrane type MMP ( MT MMP ) in CPAE cells whereas the expression of plasminogen activator inhibitor 1 ( PAI 1 ) mRNA was not changed . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Metastatic conversion correlated with upregulation of urokinase type plasminogen activator receptor RNA expression and downregulation of plasminogen activator inhibitor 1 , collagen alpha 1 ( 1 ) , and cytokeratin 14 ( K 14 ) RNA expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we investigated the presence of both LRP and urokinase type plasminogen activator receptor ( uPAR ) in glioblastoma by reverse transcriptase polymerase chain reaction ( RT PCR ) , and the cellular localization of LRP in glioblastoma tissues by immunohistochemical analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These compounds inhibited the activity of urokinase type plasminogen activator ( u PA ) which is one of serine proteases and considered to be associated with tumor invasion and metastasis in fibrin zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Human mesothelial cells ( HMCs ) have an important role in maintaining an adequately functioning fibrinolytic system in the peritoneal cavity by secreting the fibrinolytic enzymes tissue type and urokinase type plasminogen activator ( t PA and u PA ) , as well as a specific PA inhibitor , PA inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : In culture condition metastasis related genes , including matrix metalloproteinases , urokinase type plasminogen activator , and integrins alpha 2 and alpha 3 were continually expressed in T 24 but only slightly or not at all in RT 4 and KK 47 , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Transactivation of the urokinase type plasminogen activator receptor gene through a novel promoter motif bound with an activator protein 2alpha related factor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biological significance of the expression of urokinase type plasminogen activator receptors ( uPARs ) in brain tumors . ^^^ The urokinase type plasminogen activator receptor ( uPAR ) plays a critical role in the regulation of cell surface plasminogen activation in several physiological and pathological conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There are four genes affected by beta catenin overexpression ; three overexpressed genes code for two components of the AP 1 transcription complex , c jun and fra 1 , and for the urokinase type plasminogen activator receptor ( uPAR ) , whose transcription is activated by AP 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The sequences of LsPLIgamma A and LsPLIgamma B showed 65 and 74 % homology , respectively , to those of the corresponding subunits of N . naja kaouthia PLIgamma , and had two tandem patterns of cysteine residues , characteristic of the urokinase type plasminogen activator receptor ( uPAR ) and members of the Ly 6 superfamily . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Western blot analyses and co immunoprecipitations were used to investigate the association of proteins involved in epidermal growth factor and hepatocyte growth factor activation and signaling : epidermal growth factor receptor , hepatocyte growth factor receptor ( MET ) , urokinase type plasminogen activator and its receptor , and a member of the signal transducer and activator of transcription family , STAT 3 . ^^^ MET , urokinase type plasminogen activator and urokinase type plasminogen activator receptor co precipitated to varying degrees during the culture period . ^^^ This involves complex formation of the receptors with other plasma membrane components for MET ( urokinase type plasminogen activator in context of its receptor ) and activation of STAT 3 for both receptors . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A cDNA fragment containing the putative SA sequence of a target clone was fused in frame to the 5 ' end of a cDNA fragment encoding the protease domain of urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown that the glycosylphosphatidyl inositol ( GPI ) linked urokinase type plasminogen activator receptor ( uPAR ) reversibly associates with the integrins complement receptor type 3 ( CR 3 ; alphaMbeta 2 ) and CR 4 ( alphaxbeta 2 ) during leukocyte motility . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
DESIGN AND METHODS : Samples of stimulated conditioned media were collected over a period of 24 hours to determine : plasminogen activator ( PA ) and plasminogen activator inhibitor ( PAI ) activity , PAI 1 mRNA , tissue type plasminogen activator ( t PA ) antigen and urokinase type plasminogen activator ( u PA ) antigen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Angiostatic activity of synthetic inhibitors of urokinase type plasminogen activator . ^^^ We hypothesize that tumor angiogenesis can be limited by the reduction of enzymatic activity of the urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin , generated by the endogenous urokinase type plasminogen activator , is not an efficient activator of pro MMP 9 , neither the secreted pro MMP 9 nor the very low levels of pro MMP 9 associated with intact cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Four different classes of ligands are presently known , binding to distinct sites at the extracytoplasmic receptor domain : mannose 6 phosphate containing lysosomal enzymes , the non glycosylated IGF 2 , retinoic acid , and urokinase type plasminogen activator receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The role of fibrinolytic system components in thrombus formation and removal in vivo was investigated in groups of six mice deficient in urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , or plasminogen activator inhibitor 1 ( PAI 1 ) ( u PA / , t PA / or PAI 1 / , respectively ) or of their wild type controls ( u PA+ / + , t PA+ / + or PAI 1+ / + ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increase of urokinase type plasminogen activator receptor expression in human gingival fibroblasts by Porphyromonas gingivalis lipopolysaccharide . ^^^ BACKGROUND : Urokinase type plasminogen activator receptor ( uPAR ) presenting on the cell surface with glycosylphosphatidylinositol ( GPI ) anchor is a key component in the plasminogen activator ( PA ) plasmin system and plays a critical role in extracellular matrix degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Single chain urokinase type plasminogen activator ( scu PA ) possesses enzymatic activity that increases by 2 3 orders of magnitude upon binding to its cellular cofactor , the u PA receptor ( u PAR ) , hence activating an enzymatic cascade initially composed of zymogens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : High levels of urokinase type plasminogen activator ( u PA ) were demonstrated in gastric carcinomas along with inhibitors of plasminogen activators ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These results are particularly relevant in the light of the recent report that the urokinase type plasminogen activator receptor , a protein that also binds VN , has been shown to associate with the cation independent mannose 6 phosphate / IGF 2 receptor and suggest a possible role for IGF 2 in cell adhesion and invasion . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cerebral , cutaneous , and i . m . lesions consisted of blood filled cysts , histologically similar to human cavernous hemangiomas and stained positive for proliferating cell nuclear antigen , urokinase type plasminogen activator , and vascular endothelial growth factor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation mechanisms of the urokinase type plasminogen activator promoter by hepatocyte growth factor / scatter factor . ^^^ One of the effectors of HGF / SF is the urokinase type plasminogen activator , a serine protease that facilitates tumor progression and metastasis by controlling the synthesis of the extracellular matrix degrading plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We measured urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) activity in the brain of 2 3 month old and 6 8 month old mice subjected to 4 h of middle cerebral artery ( MCA ) occlusion . t PA activity was present in all non ischemic and ischemic young mouse brain . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Presence of urokinase type plasminogen activator receptor in urine of cancer patients and its possible clinical relevance . ^^^ High levels of urokinase type plasminogen activator receptor ( uPAR ) are expressed in various types of cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator and plasminogen activator inhibitor antigen in tissue extracts of paranasal sinus mucous membranes affected by chronic sinusitis and antrochoanal polyps . ^^^ We examined the effect of pH on the extraction of urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) from paranasal sinus mucous membrane associated with chronic sinusitis and antrochoanal polyps . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Assay and detection methods for urokinase type plasminogen activator and its related factors ] . ^^^ The urokinase type plasminogen activator ( u PA ) has been implicated in invasion and metastasis of various cancers including lung , breast , gastric and colon cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The catalytically inactive precursor of urokinase type plasminogen activator ( pro u PA ) induced a chemotactic response in rat smooth muscle cells ( RSMC ) through binding to the membrane receptor of urokinase ( u PA receptor [ u PAR ] ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
UVB increases urokinase type plasminogen activator receptor ( uPAR ) expression . ^^^ Keratinocytes synthesize and secrete urokinase type plasminogen activator , which binds to its specific receptor on keratinocytes . ^^^ When bound to urokinase type plasminogen activator receptor , urokinase type plasminogen activator proteolytically converts surface bound plasminogen to plasmin , which in turn cleaves many extracellular components leading to pericellular proteolysis . ^^^ As pemphigus is photoinducible , we investigated the effect of ultraviolet B on urokinase type plasminogen activator and urokinase type plasminogen activator receptor expression in the epidermal keratinocyte cell line A 431 . ^^^ Ultraviolet B increased cellular and secreted urokinase type plasminogen activator protein ( enzyme linked immunosorbent assay ) and urokinase type plasminogen activator receptor cell surface expression ( flow cytometry ) 24 h postirradiation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) play important roles in fibrinolysis , cell migration , tissue destruction , angiogenesis and tissue remodeling . u PA and t PA activity in tissue are tightly regulated by plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Saruplase ( unglycosylated human type high molecular weight single chain urokinase type plasminogen activator ) was given to 1698 patients in the open label Practical Applicability of Saruplase Study ( PASS ) , which assessed the safety and efficacy of saruplase in the treatment of acute myocardial infarction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inactivation of single chain urokinase type plasminogen activator by thrombin in human subjects . ^^^ Thrombin cleaves single chain urokinase type plasminogen activator ( scu PA ) into a virtually inactive two chain form ( tcu PA / T ) , a process that may protect a blood clot from early fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A region between 141 and 61 bp containing a proximal AP 1 is essential for constitutive expression of urokinase type plasminogen activator receptor . ^^^ An 8 . 5 kb 5 ' flanking region of the human urokinase type plasminogen activator receptor ( uPAR ) gene was cloned and the detailed uPAR promoter region defined in an 188 bp fragment between bases 141 and +47 relative to the transcription start site . 5 ' Deletion to 100 and 60 in the region abolished its promoter activity , indicating that an 81 bp segment between 141 and 61 , which contains a proximal AP 1 site at position 70 , is required for uPAR promoter activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To explore the potential of this approach , urokinase type plasminogen activator receptor ( uPAR ) , a membrane protein extensively modified post translationally , was selected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , 1 min after PHx , but not sham operation , urokinase type plasminogen activator ( u PA ) and u PA receptor were observed in the immunoprecipitates of c Met . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator cascade initiated by urokinase type plasminogen activator ( u PA ) is involved in extracellular matrix degradation during the tumor invasion process . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , the overexpression of HOXD 3 increased mRNA expressions of urokinase type plasminogen activator and transcription factors ets 1 and 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Likewise , HGF strongly enhanced urokinase type plasminogen activator activity and invasion of MMT cells through Matrigel : a 15 fold stimulation in the invasion of MMT cells was seen by HGF . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Five proteases were identified : urokinase type plasminogen activator , factor 12 , protein C , trypsinogen 4 , and a protease that we refer to as membrane type serine protease 1 ( MT SP 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Saruplase , or unglycosylated , single chain urokinase type plasminogen activator ( scu PA ) selectively activates fibrin bound plasminogen , and is subsequently converted to its two chain derivative tcu PA ( urokinase ) by plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Instead , deficiency of urokinase type plasminogen activator ( u PA / ) completely protected against rupture , whereas lack of gelatinase B partially protected against rupture . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Acetaldehyde increases the activity and gene expression of urokinase type plasminogen activator in a hepatic stellate cell line . ^^^ The aim of this study was to investigate the effect of acetaldehyde on the activity and expression of urokinase type plasminogen activator gene in a clone of hepatic stellate cells . ^^^ The treatment of the cells with doses of 100 and 175 micromol / l acetaldehyde , produced an increase in the urokinase type plasminogen activator activity not only in the cell extract , but also in conditioned medium . ^^^ However , the use of higher doses of acetaldehyde ( 250 and 350 micromol / l ) produced an inhibitory effect on the urokinase type plasminogen activator activity . ^^^ In contrast , the higher urokinase type plasminogen activator gene expression was observed with doses of 175 , 250 , and 350 micromol / l . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Therefore , expression analysis performed by Northern blotting and immunohistochemistry were extended to include IFN gamma , the IFN gamma receptor beta subunit ( IFN gammaRbeta ) , three secondary response genes induced by interaction of IFN gamma with IFN gamma receptor complexes , ie , IL 1beta converting enzyme ( ICE ) , intercellular adhesion molecule 1 ( ICAM 1 ) , and urokinase type plasminogen activator receptor ( uPAR ) , and a cytokine inducing IFN gamma expression , ie , interleukin 18 ( IL 18 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mediators of the plasminogen activator system , namely urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) , are a key complex involved in fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator inhibits alpha 4 beta 1 integrin mediated T lymphocyte adhesion to fibronectin independently of its catalytic activity . ^^^ The urokinase type plasminogen activator ( u PA ) / plasmin system plays an important role in promoting cell migration and invasion , an effect which is largely ascribed to the proteolytic activity of these enzymes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Exogenous VEGF had no significant effects on melanoma cell proliferation or on production of a transcriptional target for VEGF , urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The zymography of tumor conditioned medium showed that the treatment of tumor cells with EGF and TGF alpha resulted in the increase of type 4 collagenases , stromelysin and urokinase type plasminogen activator which was partly confirmed by immunoblot analysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study we compared the proliferation rate , the anchorage independent growth , the secretion of matrix metalloproteases and of the urokinase type plasminogen activator . ^^^ The clones expressing the c erbB 2 single chain antibody , the control cells harbouring the empty vector and the parental SK OV 3 cells they all had similar proliferation rates in the presence of 10 % serum and secreted similar amounts of matrix metalloproteases and of the urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction products revealed positive expressions of integrin alpha 5 and beta 1 , urokinase type plasminogen activator receptor ( uPAR ) , vascular endothelial growth factor and nm 23 H1 mRNAs of cell line MHCC 97 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cleavage of the Arg ( 561 ) Val ( 562 ) activation site in plasminogen by either tissue or urokinase type plasminogen activator results in formation of the fibrinolytic enzyme plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The in vitro control of MMPs 2 and 9 , TIMPs 1 and 2 , and urokinase type plasminogen activator by microglia was examined in response to a panel of chemokines ( chemotactic cytokines ) , using ELISA and zymography techniques . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cell conditioned medium was assayed for tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation of plasminogen coated bacteria occurred following incubation with either streptokinase , urokinase type plasminogen activator ( u PA ) , or a Porphyromonas gingivalis culture supernatant . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator urokinase type plasminogen activator and plasminogen activator inhibitor 1 mRNA were expressed in glioma cells during the entire tumor growth . ^^^ Our finding may suggest a functional co operation of tissue type plasminogen activator , urokinase type plasminogen activator , plasminogen activator inhibitor 1 and vascular endothelial growth factor during glioma progression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We compared the efficacy of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , activated anisoylated streptokinase plasminogen activator complex ( APSAC ) , and plasmin to dissolve surfactant incorporating fibrin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the in vitro effect of all trans retinoic acid ( ATRA ) on the production of two major fibrinolytic components , urokinase type plasminogen activator ( u PA ) and PA inhibitor 2 ( PAI 2 ) , by human blood mononuclear cells ( MNC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) functions as an important regulator of fibrinolysis by inhibiting both tissue type and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A marked in vitro selectivity was demonstrated by incubation of the doxorubicin prodrugs with a plasmin generating MCF 7 breast cancer cell line transfected with urokinase type plasminogen activator ( u PA ) in comparison with the nontransfected nonplasmin generating cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cathepsin B is able to directly facilitate invasion by degrading extracellular matrix components or to indirectly facilitate invasion by activating other matrix degrading proteases like the urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Mapping part of the functional epitope for ligand binding on the receptor for urokinase type plasminogen activator by site directed mutagenesis . ^^^ The urokinase type plasminogen activator receptor ( uPAR ) is a glycolipid anchored multidomain member of the Ly 6 / uPAR protein domain superfamily . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Of the genes studied , urokinase type plasminogen activator and plasminogen activator inhibitor 1 were regulated in RPE cells similar to fibroblasts at senescence . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The anti activating protein 1 ( AP 1 ) activity of TAC 101 caused inhibition of LLC cell invasion through the repression of expression of urokinase type plasminogen activator and its receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore chromogranin A fragment increased the fibrinolytic activities of urokinase type plasminogen activator ( u PA ) in fibrin zymograms of both PC 3 and DU 145 cells and the expression of u PA mRNA of PC 3 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cellular distribution and clinical value of urokinase type plasminogen activator , its receptor , and plasminogen activator inhibitor 2 in esophageal squamous cell carcinoma . ^^^ To assess the participation of the plasminogen activation system in the invasiveness of esophageal squamous cell carcinoma , we performed immunohistochemistry and in situ hybridization to study the distribution of a urokinase type plasminogen activator ( u PA ) , u PA receptor ( u PAR ) , and plasminogen activator inhibitor 2 ( PAI 2 ) . u PA and PAI 2 were expressed heterogeneously in cancer cells , and restricted expression was found in stromal cells , especially fibroblasts , that were located in the immediate proximity of the cancerous cells . u PAR was found only in cancer cells located at the periphery of tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BALF levels of urokinase type plasminogen activator were significantly reduced throughout , whereas the lavage concentrations of tissue type plasminogen activator did not differ from those in control subjects . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Northern blot analysis showed that curcumin inhibited serum and phorbol 12 myristate 13 acetate induced expression of tissue factor and urokinase type plasminogen activator receptor mRNA in fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin is converted from its zymogen plasminogen by tissue type or urokinase type plasminogen activator ( PA ) and degrades many components of the extracellular matrix ( ECM ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Sp 1 mediates constitutive and transforming growth factor beta inducible expression of urokinase type plasminogen activator receptor gene in human monocyte like U 937 cells . ^^^ Urokinase type plasminogen activator receptor ( uPAR ) is known to be involved in conversion of plasminogen into plasmin and its expression can be regulated by a variety of biological agents including transforming growth factor beta ( TGF beta ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Pocket protein independent repression of urokinase type plasminogen activator and plasminogen activator inhibitor 1 gene expression by E2F1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An integrative mechanism is proposed whereby gonadotrophic stimulation of urokinase type plasminogen activator secretion by the follicular contiguous ovarian surface epithelium elicits a localized increase in tissue plasmin , which activates collagenolysis and tumour necrosis factor alpha induced cell death within the formative ovulatory stigma . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Additional experiments revealed that two of the 14 mAbs neutralized and one monoclonal antibodies increased plasminogen activator inhibitor type 1 activity toward urokinase type plasminogen activator , one of its target proteases . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase type plasminogen activator ( uPAR ) ( CD 87 ) plays an important role in leukocyte adhesion and migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase mediated fibrinolysis in the synovial fluid of rheumatoid arthritis patients may be affected by the inactivation of single chain urokinase type plasminogen activator by thrombin . ^^^ BACKGROUND : Excessive fibrin deposition within the inflamed joints of rheumatoid arthritis ( RA ) patients suggests that local fibrinolysis is inefficient , which seems to be in contrast with the observed increased levels of urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our aim was to compare the level of several proteases : urokinase type plasminogen activator ( u PA ) , matrix metalloproteinase 2 ( MMP 2 ; 72 kDa type 4 collagenase , also known as gelatinase A ) , MMP 11 [ also known as stromelysin 3 ( STR 3 ) ] , and cathepsins B and L in resected non small cell lung cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As an inhibitor of serine proteinases , SERP 1 acts against tissue type plasminogen activator , urokinase type plasminogen activator , plasmin , thrombin and Factor Xa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of stromelysin and urokinase type plasminogen activator protein in resection specimens and biopsies at different stages of osteoarthritis of the knee . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The antigen of urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) were measured by ELISA , and u PA activity was evaluated by electrophoretic enzymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The sequences of these subunits contained 2 internal repeats of disulfide bonding pattern characteristic to those of urokinase type plasminogen activator receptor and members of the Ly 6 superfamily . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enhanced increases in tissue type plasminogen activator ( t PA ) antigen and activity and single chain ( sc ) urokinase type plasminogen activator ( u PA ) at maximal exercise and 5 min of recovery were observed in all age groups after training . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Human urokinase type plasminogen activator has been implicated in the regulation and control of basement membrane and interstitial protein degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Vascular endothelial cells ( ECs ) modulate the blood fibrinolytic system by secreting tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , and their inhibitor , type 1 plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effects of heat treatment on the viability and fibrinolytic potential of four cultured human carcinoma cell lines , fibrosarcoma cells ( HT 1080 ) , lung adenocarcinoma cells with highly metastatic potential ( HAL 8 ) , melanoma cells ( Bowes ) and osteosarcoma cells ( NY ) , determined by measuring their levels of urokinase type plasminogen activator ( u PA ) and its specific receptor ( u PAR ) , were investigated by comparing them with those of human umbilical vein endothelial cells ( HUVECs ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An integrative mechanism is presented whereby gonadotropic stimulation of urokinase type plasminogen activator secretion by ovarian surface epithelial cells bordering the preovulatory follicle elicits a localized increase in tissue plasmin , which activates latent collagenases and secretion of tumor necrosis factor alpha ( TNF alpha ) from thecal endothelium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This indicated that the inhibitory activity of PAI 1 was required for its anti apoptotic effect but the urokinase type plasminogen activator receptor was not involved . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Identification of a novel nuclear factor kappaB sequence involved in expression of urokinase type plasminogen activator receptor . ^^^ We have previously defined the promoter of human urokinase type plasminogen activator receptor ( uPAR ) gene in a 188 bp fragment between bases 141 and +47 relative to the translation start site . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cellular localization of membrane type serine protease 1 and identification of protease activated receptor 2 and single chain urokinase type plasminogen activator as substrates . ^^^ Protease activated receptor 2 ( PAR 2 ) and single chain urokinase type plasminogen activator are proteins that are localized to the extracellular surface and contain the preferred MT SP 1 cleavage sequence . ^^^ Single chain urokinase type plasminogen activator was activated using catalytic amounts of MT SP 1 ( 1 nm ) , but plasminogen was not cleaved under similar conditions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Wnt 1 decreased the NGF induced expression of the late response gene SCG 10 but not of the immediate early genes , fos , Nur 77 and UPAR ( urokinase type plasminogen activator receptor ) nor of the late response genes GAP 43 and collagenase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
More importantly , we found extensive areas of delayed hemorrhage near the sprouting tips of microvessels between days 7 and 14 , which temporally coincided with increased urokinase type plasminogen activator and tissue type plasminogen activator activity by zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Downregulation of the urokinase type plasminogen activator receptor through inhibition of translation by antisense oligonucleotide suppresses invasion of human glioblastoma cells . ^^^ We previously showed that downregulation of the urokinase type plasminogen activator receptor ( uPAR ) in the SNB 19 human glioblastoma cell line by the stable transfection of a plasmid expressing a 300 bp antisense sequence to the 5 ' end of the uPAR gene produced a decrease in the amount of target mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 2 and urokinase type plasminogen activator in plasma and leucocytes in patients with severe sepsis . ^^^ Proteins influencing plasminogen activation to plasmin , namely plasminogen activators tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) and their principal inhibitors , plasminogen activator inhibitor 1 ( PAI 1 ) and PAI 2 , were measured in the plasma , the polymorph and mononuclear cell fractions taken from patients with major sepsis who were entering a general intensive care unit . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mRNA expression of proteolytic proteases , including matrix metalloproteinase ( MMP ) 2 , MMP 9 , membrane type 1 ( MTI ) MMP , and urokinase type plasminogen activator ( u PA ) , were increased in the tumor and liver tissues of the isolated mice compared with the control mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A novel role for the urokinase type plasminogen activator receptor in apoptosis of malignant gliomas . ^^^ Glioblastomas express more urokinase type plasminogen activator receptor ( uPAR ) than do low grade gliomas and normal brain tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Solid and solution phase synthesis of peptidomimetic inhibitors of urokinase type plasminogen activator based on the sequence dSerAlaArg al are described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The distribution of the urokinase type plasminogen activator receptor ( uPAR ) on human glioma cells was examined as a function of culture conditions , using immunofluorescence and immunophotoelectron microscopy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( uPAR ) exists as a GPI anchored glycoprotein ( Mr=50 60 kDa ) on the surface of various cell types . ^^^ The cleaved receptor , soluble urokinase type plasminogen activator receptor ( suPAR ) , with an Mr=35 kDa has no known physiological function and can be identified circulating in the blood of normal individuals . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Applying a novel , highly specific and sensitive immunoabsorption / Western blotting technique we have identified in vitro in conditioned cell culture medium and in vivo in human urine different soluble forms of the urokinase type plasminogen activator receptor ( uPAR / CD87 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The types of agents being developed fall into five broad categories : * mutants or variants of single chain urokinase type plasminogen activator ; * mutants or variants of tissue type plasminogen activator ; * recombinant chimaeric plasminogen activators ; * conjugates of plasminogen activators and anti fibrin monoclonal antibodies ; * compounds derived from haemophagous animals . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Employing this screening method , we profiled the substrate specificities of a diverse array of proteases , including the serine proteases thrombin , plasmin , factor Xa , urokinase type plasminogen activator , tissue plasminogen activator , granzyme B , trypsin , chymotrypsin , human neutrophil elastase , and the cysteine proteases papain and cruzain . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Endothelial cells ( ECs ) synthesize plasminogen activators , tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , receptors for plasminogen activators , and a receptor for plasminogen , annexin 2 ( Ann 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunofluorescence and quantitative immunofluorescence with antibodies against alpha smooth muscle actin , desmin , matrix metalloproteinases 2 ( MMP 2 ) , MMP 9 , tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) and 2 ( TIMP 2 ) , urokinase type plasminogen activator ( u PA ) and its inhibitor 1 ( PAI 1 ) were studied with confocal microscopy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The interaction of NG 2 with plasminogen and angiostatin has very interesting functional consequences . 1 ) Soluble NG 2 significantly enhances the activation of plasminogen by urokinase type plasminogen activator . 2 ) The antagonistic effect of angiostatin on endothelial cell proliferation is inhibited by soluble NG 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Regulation of collagenase , stromelysin , and urokinase type plasminogen activator in primary pterygium body fibroblasts by inflammatory cytokines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This structural modification is associated with profound functional alterations : the rate of activation by streptokinase , tissue type plasminogen activator , urokinase type plasminogen activator and trypsin decreases and the amidolytic activity of the generated plasmin is impaired . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator up regulates the expression of its cellular receptor . ^^^ The expression of the receptor for the urokinase type plasminogen activator ( uPAR ) can be regulated by several hormones , cytokines , tumor promoters , etc . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PIP showed about 60 % sequence homology with those PLA ( 2 ) inhibitors having a urokinase type plasminogen activator receptor like domain structure . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A significant inverse correlation was found between c met mRNA expression level and survival ( in months ) of patients ( P = 0 . 007 ) , as previously shown for urokinase type plasminogen activator ( u PA ) mRNA ( P = 0 . 027 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Crystals of the urokinase type plasminogen activator variant beta ( c ) uPAin complex with small molecule inhibitors open the way towards structure based drug design . ^^^ These inhibitor complex structures have been obtained from crystals of engineered urokinase type plasminogen activator designed to obtain a crystal form open for inhibitor soaking . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A stepwise method was used to construct the models to define the best combination of risk factors among eleven prognostic factors : age , menopausal status , Scarff Bloom Richardson grade , clinical tumor size , pathological tumor size , estrogen and progesterone receptor status , urokinase type plasminogen activator , p 53 protein level , c erbB 2 protein and epidermal growth factor receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Matrix metalloproteinases ( MMPs ) and urokinase type plasminogen activator ( u PA ) / plasmin , are known to be associated with the invasive properties of various types of tumor cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : The results indicate that cartilage matrix degradation by rheumatoid synovial fibroblasts is plasmin mediated and depends on urokinase type plasminogen activator for activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Insulin like growth factor 1 and urokinase type plasminogen activator bioregulation system as a survival mechanism of prostate cancer cells in osteoblastic metastases : development of anti survival factor therapy for hormone refractory prostate cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The balance between urokinase type plasminogen activator and plasminogen activator inhibitor 1 is modified ; RNA and catalytic activity for the former are elevated while PAI 1 RNA is reduced . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PTEN overexpression downregulated genes , including integrin alpha ( 6 ) , laminin beta ( 3 ) , heparin binding epidermal growth factor like growth factor , urokinase type plasminogen activator , myb protein B , Akt 2 , and some expressed sequence tag ( EST ) clones . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
C 1 H enhanced the activation of Glu and Lys plasminogen by high molecular weight urokinase type plasminogen activator ( HMW u PA ) very effectively , but the activation by low molecular weight u PA was hardly enhanced with C 1 H . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The inactivation of single chain urokinase type plasminogen activator by thrombin on cultured human endothelial cells . ^^^ Single chain urokinase type plasminogen activator ( scu PA ) is cleaved by thrombin , resulting in an inactive molecule called thrombin cleaved two chain urokinase type plasminogen activator ( tcu PA / T ) . ^^^ Cell bound urokinase type plasminogen activator ( u PA ) was released and levels of scu PA , tcu PA / T and active two chain u PA were measured using sensitive bioimmunoassays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator expression is induced in the mouse mammary gland during development and post lactational involution . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We also investigated the expression of matrix metalloproteinases ( MMPs ) , urokinase type plasminogen activator ( u PA ) , u PA receptor ( u PAR ) and c MET in these cell lines in vitro and in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The very low density lipoprotein receptor ( VLDL R ) binds and internalizes several ligands , including very low density lipoprotein ( VLDL ) , urokinase type plasminogen activator : plasminogen activator inhibitor type 1 complexes , lipoprotein lipase , and the 39 kDa receptor associated protein that copurifies with the low density lipoprotein receptor related protein / alpha ( 2 ) macroglobulin receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As the proteases of the plasminogen activator system have been implicated in tissue remodelling , cell migration and tumour invasion , we performed an immunohistochemical study to look for evidence of alteration in the expression of plasminogen / plasmin , urokinase type plasminogen activator , tissue type plasminogen activator and alpha 2 antiplasmin in biopsies of clinically typical vulval lichen sclerosus obtained from 11 untreated adult women . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The pTRIDENT vector family has been expanded by construction of a new set of pTRIDENT based vectors containing constitutive promoters of human origin ( ubiquitin C and EF 1alpha promoters ) and selectable markers ( zeocin resistance ) and expressing different reporter genes ( secreted alkaline phosphatase ( SEAP ) and the secreted single chain urokinase type plasminogen activator ( low M ( r ) u PA ) ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The interaction of fibrinolytic components with GPIb / V / IX of platelets on thrombus formation , was investigated in mice deficient in tissue type ( tPA / ) , urokinase type plasminogen activator ( uPA / ) or plasminogen activator inhibitor 1 ( PAI 1 / ) and in their wild type control ( tPA+ / + , uPA+ / + , PAI 1+ / + ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Less microcapiral formation on tumors were observed for the treated group with a hemorrhage than the control group under microscopy . 4PyDAT significantly inhibited the production of urokinase type plasminogen activator ( u PA ) in Co26Lu ( F 55 ) cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor development is retarded in mice lacking the gene for urokinase type plasminogen activator or its inhibitor , plasminogen activator inhibitor 1 . ^^^ In vivo tumor progression in mice with targeted deficiencies in urokinase type plasminogen activator ( UPA / ) and its inhibitor , plasminogen activator inhibitor 1 ( PAI 1 / ) , was studied using a fibrosarcoma tumor model . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cancer treatment with inhibitors of urokinase type plasminogen activator and plasmin . ^^^ The urokinase type plasminogen activator plasmin system plays an important role in many normal physiological processes including clot lysis , wound healing , embryogenesis and tissue remodelling . ^^^ Inhibition of the urokinase type plasminogen activator plasmin system remains a promising , but largely untested , area of experimental cancer therapeutics . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Lesion associated expression of urokinase type plasminogen activator receptor ( uPAR , CD 87 ) in human cerebral malaria . ^^^ Recent data provide convincing evidence that the serine protease urokinase type plasminogen activator receptor ( uPAR ) is a key molecule in promoting cell adhesion and spreading . ^^^ We have now analyzed expression of urokinase type plasminogen activator receptor ( uPAR , CD 87 ) , which is part of a cell surface associated proteolytic system , in brains of eight CM patients and seven neuropathologically unaltered and diseased controls by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
On the other hand , there is now good evidence that parts of the fibrinolytic system , such as urokinase type plasminogen activator and its receptor ( `` uPAR ' ' ) , can be used as strong predictors of outcome in several types of cancer , specifically breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It was recently demonstrated that Ets 1 protein interacts with the promoter region of the genes coding for proteinases , including matrix metalloproteinase 1 ( MMP 1 ) , MMP 3 , and urokinase type plasminogen activator , suggesting that it may play an important role in the regulation of MMP expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , in vitro invasiveness , synthesis of mRNA for urokinase type plasminogen activator , and cell proliferation of both cell lines were also inhibited to 40 % by the transfection of only Sp 1 decoy ODNs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The production of PE was thus associated with the ability of tumor cells to invade the pleura , a property associated with expression of high levels of urokinase type plasminogen activator and low levels of TIMP 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Serum level of soluble urokinase type plasminogen activator receptor is a strong and independent predictor of survival in human immunodeficiency virus infection . ^^^ Human immunodeficiency virus 1 ( HIV 1 ) infection has been shown to result in up regulation of the urokinase type plasminogen activator receptor ( uPAR / CD87 ) on leukocytes in vitro and in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There was no difference in the levels of urokinase type plasminogen activator , plasminogen activator inhibitor 1 and 2 , tissue inhibitor of metalloproteinases 2 or vascular endothelial growth factor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( uPAR , CD 87 ) is a highly glycosylated 55 to 60 kDa protein anchored to the cell membrane through a glycosylphosphatidylinositol moiety that promotes the acquisition of plasmin on the surface of cells and subsequent cell movement and migration by binding urokinase type plasminogen activator . uPAR also occurs in a soluble form in body fluids and tumor extracts , and both membrane and soluble uPAR are overexpressed in patients with tumors . uPAR may be a factor in inflammatory disorders as well . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( u PA ) has been suggested to play a role in the early initiation and progression of atherosclerosis and coronary artery disease ( CAD ) ( Grenett et aL , 1998 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical expression of Ets 1 transcription factor and the urokinase type plasminogen activator is correlated with the malignant and invasive potential in meningiomas . ^^^ BACKGROUND : The Ets 1 transcription factor has been proposed to play an important role in the invasive process of tumor cells through the induction of urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The murine receptor for urokinase type plasminogen activator is primarily expressed in tissues actively undergoing remodeling . uPAR is a cellular receptor for urokinase plasminogen activator , an enzyme involved in extracellular matrix degradation during processes involving tissue remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Also , the conditioned medium of Tet FGF 2 transfectants caused proliferation , urokinase type plasminogen activator up regulation , migration , and sprouting of cultured endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In additional experiments , low concentrations of tannic acid significantly inhibited tissue type plasminogen activator , urokinase type plasminogen activator and plasmin activity in a concentration dependent manner over the range 0 . 25 200 micromol l ( 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
ECs synthesize fibrinolytic proteins , tissue plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our previous works demonstrated that ligands of macrophage scavenger receptor ( MSR ) induce protein kinases ( PKs ) including protein tyrosine kinase ( PTK ) and up regulate urokinase type plasminogen activator expression ( Hsu , H . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These cells showed reduced invasiveness that paralleled decreased mRNA levels of urokinase type plasminogen activator and its receptor , tissue type plasminogen activator , and plasminogen activator inhibitor 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , the PAI 1 activity toward urokinase type plasminogen activator and tissue type plasminogen activator was significantly prolonged in the presence of alpha ( 1 ) acid glycoprotein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previous investigations suggested a cell cycle dependent expression of urokinase type plasminogen activator ( u PA ) in epithelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We recently demonstrated that urokinase type plasminogen activator , an extracellular protease to which no DNA repair functions have been assigned , was induced by MNNG . ^^^ Here , we show that the physiological inhibitor of urokinase type plasminogen activator , PAI 1 , is also induced by MNNG in a p 53 dependent fashion , because MNNG induced PAI 1 in p 53 expressing cells but not in p 53 / cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical analyses of the healing tissues and an analysis of the periodontal wound healing fluid by ELISA were carried out for the detection of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , and 2 plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Development of serine protease inhibitors displaying a multicentered short ( < 2 . 3 A ) hydrogen bond binding mode : inhibitors of urokinase type plasminogen activator and factor Xa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Free and immobilized vitronectin and fibronectin stimulated the proliferation of cells under serum free conditions and the production and release of urokinase type plasminogen activator , and increased the release of the activated forms of matrix metalloproteinase 2 and matrix metalloproteinase 9 in an alpha ( 5 ) beta ( 3 ) integrin dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At the cellular level , particulate nickel subsulfide inhibits fibrinolysis by transcriptionally inducing expression of plasminogen activator inhibitor ( PAI ) 1 , an inhibitor of the urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical staining for urokinase type plasminogen activator was positive in primary sigmoid colon cancer and splenic metastasis , but negative in lymph node metastasis ; results that possibly reflect the difference in progenitor cells between splenic metastasis and lymph node metastasis or the difference in the microenvironment of cancer cells between the spleen and lymph nodes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of urokinase type plasminogen activator by the anthracycline antibiotic in human RC K 8 lymphoma and H 69 lung carcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of matrix metalloproteinase 9 , stromelysin 1 and urokinase type plasminogen activator was also observed in the mastitic tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prognostic impact of proteolytic factors ( urokinase type plasminogen activator , plasminogen activator inhibitor 1 , and cathepsins B , D , and L ) in primary breast cancer reflects effects of adjuvant systemic therapy . ^^^ EXPERIMENTAL DESIGN : Antigen levels of urokinase type plasminogen activator , plasminogen activator inhibitor 1 ( PAI 1 ) , Cathepsins B , D , and L were determined using immunochemical assays in primary tumor tissue of 276 patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator system predicts risk of cardiovascular events in patients with angina pectoris : results of the ECAPTURE study . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunocytochemistry revealed enhanced accumulation of macrophages in atherosclerotic lesions of ApoE ( / ) : MMP 3 ( + / + ) mice ( P < 0 . 01 ) and expression of urokinase type plasminogen activator ( u PA ) and MMP 3 colocalizing with macrophages . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We observed a preferential expression of MAGE A in patients at a higher risk of recurrence : those harboring tumors with high levels of the protease urokinase type plasminogen activator and its inhibitor plasminogen activator inhibitor 1 , high score of the Ki 67 proliferation antigen , and lesser degree of differentiation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Experimental data indicate that urokinase type plasminogen activator ( u PA ) contributes significantly to endogenous fibrinolysis and vascular remodeling in proportion to its local concentrations . ^^^ Single chain urokinase type plasminogen activator ( scu PA ) was measured by bioimmunoassay , and total u PA antigen ( including scu Pa and two chain urokinase type plasminogen activator complexed with inhibitors ( tcu PA ) ) by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
For instance , both single chain urokinase type plasminogen activator ( scu PA ) and staphylokinase have a high preference for this type of plasminogen , which explains their fibrin selective action . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Down regulation of integrin alpha ( 5 ) beta ( 3 ) expression and integrin mediated signaling in glioma cells by adenovirus mediated transfer of antisense urokinase type plasminogen activator receptor ( uPAR ) and sense p 16 genes . ^^^ The expression of urokinase type plasminogen activator receptor ( uPAR ) was recently shown to co regulate with the expression of alpha ( 5 ) beta ( 3 ) integrin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Motility assays , as well as zymographic assays carried out to analyze gelatinolytic metalloproteinases and urokinase type plasminogen activator , were performed in one of the endothelial cell lines . ^^^ Non cytotoxic PTX concentrations assayed in one of the endothelial cell lines did not inhibit its in vitro cell motility nor its gelatinase secretion , but its low molecular weight urokinase type plasminogen activator expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This hypothesis was confirmed because recombinant SERPINB 12 inhibited human trypsin and plasmin but not thrombin , coagulation factor Xa , or urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Further studies are required to establish with confidence the associations for plasminogen activator inhibitor type 1 , urokinase type plasminogen activator , and other fibrinolytic variables . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator up regulates its own expression by endothelial cells and monocytes via the u PAR pathway . ^^^ Signal transduction by urokinase type plasminogen activator ( u PA ) bound to its cell receptor has been well established . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this review , we will discuss the defects in infarct healing that have been observed in null mutants for plasminogen , urokinase type plasminogen activator ( u PA ) , matrix metalloproteinases ( MMPS ) , thrombospondin 2 and dishevelled 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Differential effects of acute and chronic wound fluids on urokinase type plasminogen activator , urokinase type plasminogen activator receptor , and tissue type plasminogen activator in cultured human keratinocytes and fibroblasts . ^^^ Urokinase type plasminogen activator , tissue type plasminogen activator , urokinase type plasminogen activator receptor , and plasminogen activator inhibitor 1 expression were studied . ^^^ The results indicated that the later the acute wound fluid was collected , the greater the urokinase type plasminogen activator and the lower the plasminogen inhibitor 1 level in treated cells . ^^^ In contrast , the level of urokinase type plasminogen activator receptor remained stable irrespective of wound fluid treatment . ^^^ Immunostaining for urokinase type plasminogen activator of acute wound fluid treated cells showed a disseminated punctate pattern over the cell surface , but with chronic wound fluid , urokinase type plasminogen activator was localized to focal contacts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Identification of the epidermal growth factor like domains of thrombomodulin essential for the acceleration of thrombin mediated inactivation of single chain urokinase type plasminogen activator . ^^^ Single chain urokinase type plasminogen activator ( scu PA ) can be cleaved by thrombin into a virtually inactive form called thrombin cleaved two chain urokinase type plasminogen activator ( tcu PA / T ) , a process accelerated by thrombomodulin , which contains six epidermal growth factor ( EGF ) like domains . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombolytic efficacy of lonomin 5 ( LV ) , a protein isolated from Lonomia achelous caterpillars haemolymph , administered either as a single intravenous bolus or as a continuous infusion , was evaluated in a rabbit jugular vein thrombosis model , and compared with those of single chain tissue type plasminogen activator ( sct PA ) and two chain urokinase type plasminogen activator ( tcu PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human tissue factor pathway inhibitor 2 ( TFPI 2 ) is a Kunitz type serine protease inhibitor that inhibits plasmin , trypsin , chymotrypsin , cathepsin G , and plasma kallikrein but not urokinase type plasminogen activator , tissue plasminogen activator , or thrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The downregulation of transcriptional AP 1 activity by curcumin as seen in the dual luciferase assay caused inhibition of LLC cell invasion through the repression of expression of the mRNAs for urokinase type plasminogen activator ( u PA ) and its receptor ( u PAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Downregulation of urokinase type plasminogen activator receptor ( uPAR ) induces caspase mediated cell death in human glioblastoma cells . ^^^ Urokinase type plasminogen activator receptors ( uPARs ) play an important role in tumor invasion by localizing degradative enzymes at the invasive zone . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We reported previously that the stable transfection of the dominant negative form of Ets 1 ( Ets DN ) in the glioma cell line U 251 induced down regulation of urokinase type plasminogen activator mRNA expression and invasiveness ( M . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The content of urokinase type plasminogen activator increased , while the concentration of tissue type plasminogen activator decreased in bone tumors of various histological compositions compared to osteochondral exostoses . ^^^ Expression of urokinase type plasminogen activator increased in patients with primary osteosarcomas characterized by early generalization of the pathological process . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , ET 1 induced overexpression of urokinase type plasminogen activator , its receptor , and plasminogen activator inhibitor type 1 and 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen can be converted to plasmin either via the tissue type plasminogen activator ( t PA ) or via the urokinase type plasminogen activator ( u PA ) / u PA receptor ( u PAR ) pathway . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of hK 4 ( prostase ) , a prostate specific serine protease : activation of the precursor of prostate specific antigen ( pro PSA ) and single chain urokinase type plasminogen activator and degradation of prostatic acid phosphatase . hK 4 ( prostase , KLK 4 ) , a recently cloned prostate specific serine protease and a member of the tissue kallikrein family , is a zymogen composed of 228 amino acid residues including an amino terminal propiece , Ser Cys Ser Gln . ^^^ The rhK 4 was inhibited by aprotinin ( 6 kDa ) , forming an equimolar 27 kDa complex . rhK 4 readily activated both the precursor of PSA ( pro PSA ) and single chain urokinase type plasminogen activator ( scuPA , pro uPA ) . rhK 4 also completely degraded prostatic acid phosphatase but failed to cleave serum albumin , another protein purified from human seminal plasma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There was a significant correlation between NF kappaB activation ( nuclear translocation of RelA ) and expression of urokinase type plasminogen activator , an invasion related factor and target of NF kappaB in tumor cells ( rho = 0 . 393 ; P = 0 . 0013 ) . ^^^ CONCLUSIONS : Collectively , the data show that NF kappaB is constitutively activated in human gastric carcinoma tissues and suggest that NF kappaB activity is related to tumor progression due to its transcriptional regulation of invasion related factors such as urokinase type plasminogen activator . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We conducted an anatomical and histological study of the perineum of the normal mouse and of a transgenic mouse strain deficient in urokinase type plasminogen activator ( uPA / ) that was previously reported to develop a high incidence of rectal prolapse . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our previous studies showed that the urokinase type plasminogen activator receptor ( uPAR ) and the p 16 tumor suppressor gene play a significant role in glioma invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : The plasma level of tissue factor ( TF ) , tissue factor pathway inhibitor ( TFPI ) , thrombin antithrombin complex ( TAT ) , plasmin antiplasmin complex ( PAP ) , urokinase type plasminogen activator ( u PA ) , urokinase type plasminogen activator receptor ( u PAR ) and the TF level of bone marrow blasts lysate were measured by ELISA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The recombinant PAI 1 / GFP had significant inhibition activity on urokinase type plasminogen activator and displayed autonomous fluorescence . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression and significance of urokinase type plasminogen activator , its receptor , and type 2 inhibitor in giant cell tumor of bone ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have examined expression of the components of the plasminogen activation system in human astrocytoma U 373 MG cells and found that interleukin 1beta ( IL 1beta ) , tumour necrosis factor alpha TNF alpha ) , interferon gamma ( INF gamma ) and epidermal growth factor ( EGF ) specifically regulate the expression of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor type 1 ( PAI 1 ) and protease nexin 1 ( PN 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
SAMY expression and detection was fully compatible with established SEAP ( human secreted alkaline phosphatase ) and u PA ( LMW ) ( low molecular weight urokinase type plasminogen activator ) reporter systems and could be used to quantify expression of up to three independent genes in one culture supernatant . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A study of the S 1 binding of lead 5 methylthiothiophene amidine 3 , an inhibitor of urokinase type plasminogen activator , was undertaken by the introduction of a variety of substituents at the thiophene 5 position . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have reported that alpha 1 : Ser ( 2091 ) Arg ( 2108 ) , a peptide derived from the alpha 1 chain of laminin 1 , triggers protein kinase C dependent activation of MAPK ( erk1 / 2 ) , leading to the up regulation of macrophage urokinase type plasminogen activator and matrix metalloproteinase ( MMP ) 9 expression . ^^^ A peptide from the first loop of the globular domain ( alpha 1 : Ser ( 2179 ) Ser ( 2198 ) ) triggered the phosphorylation of MAPK ( erk1 / 2 ) and stimulated the expression of macrophage urokinase type plasminogen activator and MMP 9 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Association of nuclear beta catenin with the T cell factor ( TCF ) / lymphoid enhancer factor ( LEF ) family of transcription factors promotes the expression of several compounds that have important roles in the development and progression of colorectal carcinoma , namely : c myc , cyclin D 1 , gastrin , cyclooxygenase ( COX ) 2 , matrix metalloproteinase ( MMP ) 7 , urokinase type plasminogen activator receptor ( aPAR ) , CD 44 proteins , and P glycoprotein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( urokinase ) plays a pivotal role in the regulation of cell adhesion and migration during tissue remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effect of malondialdehyde acetaldehyde protein adducts on the protein kinase C dependent secretion of urokinase type plasminogen activator in hepatic stellate cells . ^^^ We observed that BSA MAA induces the increased secretion of urokinase type plasminogen activator , a key component of the plasmin generating system , and that PKC activation is necessary for this enhanced urokinase type plasminogen activator secretion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To investigate the potential role of tissue type plasminogen activator ( t PA ) or urokinase type plasminogen activator ( u PA ) in development of adipose tissue , we have used a nutritionally induced obesity model in t PA ( t PA / ) and u PA ( u PA / ) deficient mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since our discovery of the CR 3 FcgammaRIIIB interaction , the plasma membrane protein repertoire of beta 1 , beta 2 , and beta 3 integrins has grown to include : FcgammaRIIA ( CD 32 ) , uPAR ( urokinase type plasminogen activator receptor ; CD 87 ) , CD 14 , voltage gated K+channels ( Kv1 . 3 ) , integrin associated protein ( IAP ) , CD 98 , tetraspans ( TM4SF ) , insulin receptors , and PDGFbeta receptors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To compare the effects of thrombin on the expression of the tissue factor ( TF ) and urokinase type plasminogen activator receptor ( uPAR ) mRNA in the cultured vascular endothelial cells ( VEC ) and U 937 cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
One consists of four parts : a portion of the low molecular mass single chain urokinase type plasminogen activator ( scu PA 32K , residues 144 411 ) , a 15 mer linker sequence , the C terminal amino acid sequence ( residues 53 65 ) of hirudin ( Hir ) , and an RGD sequence derived from the leech protein decorsin , i . e . scu PA ( 32 k ) linker Hir ( residues 53 65 ) RGD peptide . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In total , 22 functional and immunologic assays were applied to plasma obtained from domestic pigs , and the following blood coagulation and fibrinolysis variables were measured : prothrombin time , activated partial thromboplastin time , tissue factor , tissue factor pathway inhibitor , factor 7 , protein C , protein S , prothrombin fragment 1+2 , antithrombin , thrombin antithrombin complexes , fibrinogen , soluble fibrin , urokinase type plasminogen activator , plasmin inhibitor , plasminogen activator inhibitor 1 , and D dimer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Chemokine inducible transcripts could be clustered in functional groups , including selected cytokines and receptors ( e . g . , IL 1beta , CCL2 / monocyte chemotactic protein 1 , and the CCL 5 receptor CCR 1 ) and molecules involved in extracellular matrix recognition and digestion ( e . g . , CD 44 splice transcripts , urokinase type plasminogen activator receptor , matrix metalloprotease ( MMP ) 9 , and MMP 19 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Hypoxia promotes lymph node metastasis in human melanoma xenografts by up regulating the urokinase type plasminogen activator receptor . ^^^ The present study demonstrates that hypoxia promotes spontaneous lymph node metastasis in R 18 human melanoma xenografts by up regulating the urokinase type plasminogen activator receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Diagnostic significance of measurement of the receptor for urokinase type plasminogen activator on granulocytes and in plasma from patients with paroxysmal nocturnal hemoglobinuria ] . ^^^ OBJECTIVE : To investigate the expression levels of the receptor for urokinase type plasminogen activator ( uPAR ) on granulocytes and the soluble uPAR ( suPAR ) level in plasma from patients with paroxysmal nocturnal hemoglobinuria ( PNH ) and its clinical application in the diagnosis of this disorder . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The activity of MMP 2 and 9 , and expression of urokinase type plasminogen activator were investigated with gelatin zymography and immunoblot , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To study the invasion related molecule urokinase type plasminogen activator receptor ( u PAR ) expressed by disseminated tumor cells as a biologic predictor of poor survival in a large prospective series of patients with gastric cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expressions of MMP 2 and MMP 9 and their activators ( membrane type 1 MMP and urokinase type plasminogen activator ) were thought to be associated with the growth , invasion and metastasis of the human lung adenocarcinoma cell lines examined . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase type plasminogen activator receptor ( uPAR ) plays an important role in cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In an in vivo model of atherosclerosis , using mice that had a combined deficiency of apolipoprotein E ( ApoE ) and urokinase type plasminogen activator ( u PA ) and that were maintained on a cholesterol rich diet , it was observed that u PA deficiency protects against aneurysm formation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The direct and indirect contributions of the urokinase type plasminogen activator receptor ( uPAR ) system in inflammatory processes , as they relate to recruitment of leukocytes , define novel functions and could serve as therapeutic targets for related vasculopathies . ^^^ Urokinase type plasminogen activator was identified as a potent mitogen for vascular smooth muscle cells , an observation that was independent of the presence of uPAR and its proteolytic activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A central mechanism for regulating plasmin generation is through the binding of the two plasminogen activators to specific cellular receptors : urokinase type plasminogen activator to the glycolipid anchored membrane protein uPAR , and tissue plasminogen activator to a type 2 transmembrane protein recently identified on vascular smooth muscle cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Diagnostic significance of measurement of the receptor for urokinase type plasminogen activator on granulocytes and in plasma from patients with paroxysmal nocturnal hemoglobinuria . ^^^ The receptor for urokinase type plasminogen activator ( uPAR ) also is attached to the cell membrane by a GPI anchor , and that soluble uPAR ( suPAR ) is present in plasma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
No evidence was found for the presence of urokinase type plasminogen activator , plasminogen activator inhibitors or plasmin inhibitors in bovine oocyte cortical granule extracts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ovarian surface epithelial cells in close contact with the apical wall of preovulatory ovine follicles secrete a urokinase type plasminogen activator in response to surge concentrations of ( locally delivered ) gonadotrophins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The novel serine protease HGF activator and urokinase type plasminogen activator ( u PA ) are responsible for the latter extracellular processing . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Antigen levels of urokinase type plasminogen activator and its inhibitors in primary breast cancer . ^^^ Primary Breast Cancer , Urokinase Type Plasminogen Activator , Inhibitors The aim of the study was to monitor urokinase plasminogen activator antigen concentrations and its type 1 ( PAI 1 ) and type 2 ( PAI 2 ) inhibitors in histologically defined forms of primary breast cancer and a comparison with these antigens levels in normal tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator ( u PA ) receptor ( u PAR ) is under hypoxic control and cooperates with other serine proteases of the blood coagulation pathways that may extravasate in the tumor milieu as a result of hypoxia simulated increase of vessel permeability . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our focus was to develop an anti angiogenic drug possessing the inhibitory activity of urokinase type plasminogen activator ( u PA ) production . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since different enzymatic mechanisms were described to explain the generation of angiostatin , we also studied gelatinase and urokinase type plasminogen activator secretion in conditioned media by zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Clinical significance of plasma tissue factor pathway and urokinase type plasminogen activator system in cancer patients . ^^^ OBJECTIVE : To evaluate variations in the plasma tissue factor ( TF ) and urokinase type plasminogen activator ( u PA ) system and their relationship with clinical cancer type , pathological classification and metastatic status in cancer patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Up regulation of the urokinase type plasminogen activator receptor by monocyte chemotactic proteins . ^^^ The urokinase type plasminogen activator receptor ( uPAR ) is a multifunctional molecule involved in migration and adhesion of leukocytes to sites of inflammation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast , binding and activation of plasminogen by single chain urokinase type plasminogen activator ( scu PA ) at the surface of blood monocytes and alveolar macrophages were not different from those of control values . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , the primary physiological inhibitor of both tissue type plasminogen activator and urokinase type plasminogen activator in plasma , is a well established risk factor in thrombotic diseases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of the binding of urokinase type plasminogen activator to the asialoglycoprotein receptor . ^^^ In order to study the role of the asialoglycoprotein receptor ( ASGPr ) in the rapid plasma clearance of urokinase type plasminogen activator ( u PA ) , a microtiter plate binding assay was developed using ASGPr purified from rat liver extracts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Among the most highly regulated genes in endothelial cells were the cytokines interleukin 6 and GRO alpha ; several genes affecting endothelial / vascular growth and remodeling were also induced , including plasminogen , thrombomodulin , the urokinase type plasminogen activator receptor , and to a modest extent vascular endothelial growth factor C . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Quantitative reverse transcriptase polymerase chain reaction showed increased mRNA levels of MMP 12 , MMP 14 , and urokinase type plasminogen activator after allergen exposure in the lung extracts of WT mice but not in MMP 9 deficient mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MMP 9 and urokinase type plasminogen activator were expressed by Isreco 1 cells , and bombesin did not significantly alter their level of secretion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PAI 1 inhibits tissue plasminogen activator and urokinase type plasminogen activator , resulting in reduced plasminogen activity and attenuated fibrinolysis and proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
As demonstrated by ELISA , we found that the secretion of matrix metalloproteinase ( MMP ) 2 and MMP 9 , urokinase type plasminogen activator and plasminogen activator inhibitor type 1 and 2 was upregulated by ET 1 in a dose dependent manner in the HEY cell line . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Characterization of urokinase type plasminogen activator modified by phenylglyoxal ] . ^^^ A chemical modification of single chain urokinase type plasminogen activator ( scu PA ) with phenylglyoxal under mild conditions resulted in the scu PA derivatives with various numbers of the modified Arg residues . ^^^ The neutralization of positively charged Arg residues in this cluster decreases the affinity of scu PA and the double chain urokinase type plasminogen activator for PAI 1 , which results in an enhancement of the stability in plasma and the fibrinolytic efficiency of the activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Decreased expression of intercellular adhesion molecule 1 ( ICAM 1 ) and urokinase type plasminogen activator receptor ( uPAR ) is associated with tumor cell spreading in vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The genome wide expression of the two samples exhibited partial overlaps ; different sets of up regulated genes but the same set of down regulated genes . 8 Cl adenosine treatment up regulated genes involved in differentiation and development ( LIM protein , connexin 26 , neogenin , neurofilament triplet L protein and p 21 ( WAF1 / CIP1 ) ) and immune response such as natural killer cells protein 4 , and down regulated ones involved in proliferation and transformation ( transforming growth factor beta , DYRK 2 , urokinase type plasminogen activator and proteins involved in transcription and translation ) which were in close parallel with those by 8 Cl cAMP . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Role of beta ( 3 ) endonexin in the regulation of NF kappaB dependent expression of urokinase type plasminogen activator receptor . ^^^ Previous studies showed that beta ( 3 ) integrins regulate expression of the urokinase type plasminogen activator receptor ( uPAR ) through outside in signalling involving the cytoplasmic domain . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we examined the effect of platelet endothelial cell interactions on the expression of urokinase type plasminogen activator ( u PA ) by human microvascular endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The objective of this study was to evaluate the role of plakoglobin in the pathogenesis of acantholysis in pemphigus vulgaris ( PV ) and its relation with the urokinase type plasminogen activator receptor ( uPAR ) expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparison of metastatic and nonmetastatic cells revealed several significant alterations in the expression of metastasis related genes , including up regulation of the urokinase type plasminogen activator receptor , integrin beta 1 , membrane type 1 matrix metalloproteinase , and down regulation of protease activated receptor 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : Expression of matrix metallsoproteinase ( MMP ) 2 , MMP 9 , tissue inhibitor of metalloproteinases ( TIMP ) 1 and urokinase type plasminogen activator ( u PA ) , other than that of TIMP 2 and plasminogen activator inhibitor type ( PAI ) 1 , was observed in normal trophoblastic cells in in vitro culture . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Molecular proximity of seprase and the urokinase type plasminogen activator receptor on malignant melanoma cell membranes : dependence on beta 1 integrins and the cytoskeleton . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Transient ( 60 minute ) adhesion of thrombin prestimulated platelets enhanced HUVEC expression of urokinase type plasminogen activator receptor and membrane type 1 matrix metalloproteinase ( MT 1 MMP ) ( reverse transcriptase polymerase chain reaction , flow cytometry ) and secretion of urokinase type plasminogen activator , tissue type plasminogen activator , and MMP 1 ( ELISA ) and induced proteolytic activity via MMP 2 and MMP 9 ( gelatin zymography ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have compared the effects of maspin with those of PAI 1 in a range of situations in which plasminogen activation is potentiated , reflecting the biological context of this proteolytic system : urokinase type plasminogen activator bound to its receptor on the surface of tumor cells , tissue type plasminogen activator specifically bound to vascular smooth muscle cells , fibrin , and the prion protein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Previous studies demonstrated that integrin alpha ( M ) beta ( 2 ) ( CD11b / 18 , Mac 1 ) forms a physical complex with the urokinase type plasminogen activator receptor ( uPAR / CD87 ) on leukocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Clinical relevance of urokinase type plasminogen activator and its inhibitor type 1 ( PAI 1 ) in squamous cell carcinoma of the uterine cervix . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Hormone receptor status , Ki 67 , S phase fraction , DNA ploidy , HER 2 / neu , p 53 , epidermal growth factor receptor , urokinase type plasminogen activator , plasminogen activator inhibitor 1 , bone marrow micrometastases as well as patient age , menopausal status , tumor site , tumor size , histologic type , tumor grade , carcinoma in situ , multifocality , and lymph vascular invasion ( LVI ) were studied to predict axillary lymph node status . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In cells lacking Sp 1 and KLF 6 , transfected KLF 6 and Sp 1 cooperatively transactivated the endoglin promoter and those of collagen alpha 1 ( 1 ) , urokinase type plasminogen activator , TGF beta 1 , and TGF beta receptor type 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Gene transfer of the urokinase type plasminogen activator receptor targeted matrix metalloproteinase inhibitor TIMP 1 . ^^^ By binding to the u PA receptor this protein will not only anchor the TIMP 1 moiety directly to the cell surface , it will also prevent the local activation of plasminogen by blocking the binding of urokinase type plasminogen activator ( u PA ) to its receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We further studied the relationship of sCD 13 with tumor load as well as with vascular endothelial growth factor ( VEGF ) , endostatin , matrix metalloproteinase ( MMP ) 2 , MMP 9 , urokinase type plasminogen activator , and plasmin . ^^^ The concentrations of VEGF and endostatin and the activities of urokinase type plasminogen activator and MMP 9 , but not MMP 2 , were significantly higher in TF compared with all other effusions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We also measured D dimer , lipid pattern , soluble E selectin , platelet surface P selectin , prothrombin fragments 1 + 2 ( F 1 + 2 ) , lipoprotein ( a ) , factor 7 , von Willebrand factor , plasma insulin , fibrinogen , homocysteine , thrombin activable fibrinolysis inhibitor ( TAFI ) activity , thrombomodulin , factor 13 , urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is a factor in urokinase type plasminogen activator proteolytic system , which is important for tumour invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The low density lipoprotein receptor related protein ( LRP 1 ) binds and mediates the endocytosis of multiple ligands , transports the urokinase type plasminogen activator receptor ( uPAR ) and other membrane proteins into endosomes , and binds intracellular adaptor proteins involved in cell signaling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : TNF alpha increased secretion of urokinase type plasminogen activator up to 3 fold of basal unstimulated production . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study demonstrates that recombinant headpin indeed inhibits cathepsins K and L , but not chymotrypsin , elastase , trypsin , subtilisin A , urokinase type plasminogen activator , plasmin , or thrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine whether these factors contribute to the distinct etiologies of fibrolamellar hepatocellular carcinoma and traditional hepatocellular carcinoma , we assayed hepatocyte growth factor , the hepatocyte growth factor receptor , and two hepatocyte growth factor activators , hepatocyte growth factor activator and urokinase type plasminogen activator , in hepatocellular carcinoma , fibrolamellar hepatocellular carcinoma , cirrhotic liver and normal liver . ^^^ In addition , we examined the urokinase type plasminogen activator receptor , the type 1 plasminogen activator inhibitor , plasmin , fibrinogen , and the type 4 matrix metalloproteinases . ^^^ Antibodies against urokinase type plasminogen activator , urokinase type plasminogen activator receptor , hepatocyte growth factor and hepatocyte growth factor receptor were used to analyze immunoperoxidase stained slides from the paraffin blocks . ^^^ Western blot analyses using antibodies against hepatocyte growth factor , hepatocyte growth factor receptor , phosphotyrosine , hepatocyte growth factor activator , urokinase type plasminogen activator receptor , urokinase type plasminogen activator , plasminogen activator inhibitor 1 , fibrinogen and plasmin were performed on membrane enriched fractions from the frozen tissue , as was collagen zymography for matrix metalloproteinase 2 and matrix metalloproteinase 9 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this study was to determine the effects of hypoxia on mRNA levels , cell associated and secreted protein concentration , activity , and protein complex formation of urokinase type plasminogen activator , its receptor , and plasminogen activator inhibitor type 1 in corneal epithelium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator and its receptor ( u PAR ) contribute to prostate cancer metastases by promoting extracellular matrix degradation and growth factor activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A 15 mer asODNs targeted against the translation start site of UPAR ( urokinase type plasminogen activator receptor ) mRNA were introduced into CCL 229 cells by lipid mediated DNA transfection and the variation of the levels of uPAR mRNA , uPAR antigen expression of the levels of uPAR mRNA , uPAR antigen expression on the cell sruface and invasion properties were observed by reverse transcription polymerase chain reaction ( RT PCR ) , flowcytometry ( FCM ) and aminion invasion assay , the morphological feature of the cell after asODNs treatment was observed by scanning electron microscope ( SEM ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this article , we review the role of proteolytic enzymes , urokinase type plasminogen activator and matrix metalloproteases , for diffuse infiltrative growth of glioblastoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At the membrane level , gelatinase MMP 2 mainly the activated form was restrained by CNTF and BDNF to a residual 34 % with both factors ; membrane type 1 MMP was down regulated to 50 % ( 10 h ) and 34 % ( 24 h ) with both factors ; and urokinase type plasminogen activator was restrained mainly by BDNF to 70 % . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Most cancer cells secrete the urokinase type plasminogen activator , which converts cell bound plasminogen to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor expression in adenoid cystic carcinoma of the skull base . ^^^ The urokinase type plasminogen activator and its receptor ( uPAR ) have an important role in tumor invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of factors of the urokinase type plasminogen activation system , including the urokinase type plasminogen activator ( u PA ) and the plasminogen activator inhibitor type 1 ( PAI 1 ) , which have been described to be frequently implicated in the process of degradation of the extracellular matrix during the metastatic process , were also analyzed . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Exogenous VEGF A ( 165 ) also decreased the cell surface expression of the urokinase type plasminogen activator ( a molecule required for invasion ) by the breast carcinoma and trophoblast cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A subsequent gene specific RT PCR screen for 22 serine proteases confirmed expression of these 5 genes and identified 7 additional serine protease genes expressed by human endothelial cells , urokinase type plasminogen activator , protein C , TMPRSS 2 , hepsin , matriptase / MT SP 1 , dipeptidylpeptidase 4 , and seprase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In vascular injury models , Serp 1 altered early cellular plasminogen activator ( tissue plasminogen activator ) , inhibitor ( PAI 1 ) , and receptor ( urokinase type plasminogen activator ) expression ( p < 0 . 01 ) . ^^^ Treatment of endothelial cells with antibody to urokinase type plasminogen activator and vitronectin blocked Serp 1 induced changes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
During incubation with urokinase type plasminogen activator , the polymers were slowly converted to reactive centre cleaved monomers , indicating substrate behaviour of the terminal PAI 1 molecules in the polymers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of urokinase type plasminogen activator receptor ( uPAR ) to the mannose 6 phosphate / insulin like growth factor 2 receptor : contrasting interactions of full length and soluble forms of uPAR . ^^^ Urokinase type plasminogen activator receptor ( uPAR ) binding by the mannose 6 phosphate / insulin like growth factor 2 receptor ( Man 6 P / IGF2R ) is considered important to Man 6 P / IGF2R tumor suppressor function via regulation of cell surface proteolytic activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cellular receptor for urokinase type plasminogen activator , uPAR , plays a central role in both cell surface associated proteolysis and cellular adhesion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purpose of this study was to investigate the association between tumor tissue levels of total tissue inhibitor of metalloproteinases 1 ( TIMP 1 ) and prognosis in patients with primary breast cancer and to analyze whether measurement of TIMP 1 in tumor extracts added prognostic information to that obtained from measurements of urokinase type plasminogen activator and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To establish an introductory understanding of the quantitative mRNA expression of MT SP 1 and to correlate these levels with urokinase type plasminogen activator receptor ( uPAR ) , a key component of extracellular proteolysis , quantitative RT PCR was carried out . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
After 6 h incubation , the tissues were collected , NF kappaB DNA binding activity was assessed by gel shift binding assays , and matrix metalloproteinase 9 and urokinase type plasminogen activator activity were determined by zymography . ^^^ Treatment of fetal membranes with NAC significantly suppressed lipopolysaccharide stimulated type 2 phospholipase A ( 2 ) release and content ; PGF ( 2alpha ) , IL 6 , IL 8 , TNFalpha , and 8 isoprostane release ; and matrix metalloproteinase 9 and urokinase type plasminogen activator enzyme activity and suppressed NF kappaB DNA binding activity ( by ANOVA , P < 0 . 05 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Antithrombotic thrombocytes : ectopic expression of urokinase type plasminogen activator in platelets . ^^^ To test our hypothesis , we directed murine urokinase type plasminogen activator transgene expression to platelets using a platelet factor 4 promoter . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
While matrix metalloproteinases , urokinase type plasminogen activator , and cytokines play a major role in invasion and angiogenesis , chemokines such as stromal derived factor 1alpha ( SDF 1alpha ) and their receptors such as CXCR 4 are thought to play a critical role in motility , homing , and proliferation of cancer cells at specific metastatic sites . ^^^ We and others have previously reported that the extracellular signal activated transcription factor NF kappaB up regulates the expression of matrix metalloproteinases , urokinase type plasminogen activator , and cytokines in highly metastatic breast cancer cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The serpin plasminogen activator inhibitor 1 ( PAI 1 ) is a fast and specific inhibitor of the plasminogen activating serine proteases tissue type and urokinase type plasminogen activator and , as such , an important regulator in turnover of extracellular matrix and in fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The study also revealed that tetranectin did not interact with the kindred proteins : macrophage stimulating protein , urokinase type plasminogen activator and prothrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor crosslinking in an NK cell line increases integrin surface expression by the MAP kinase / ERK 1 / 2 signaling pathway . ^^^ Urokinase type plasminogen activator receptor ( uPAR ) is attached to cell membranes by a glycosylphosphatidylinositol ( GPI ) anchor , and as such is devoid of an intracellular domain , but is nevertheless able to initiate signal transduction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of nonphysiological protein and peptide substrates to proteases : differences between urokinase type plasminogen activator and trypsin and contributions to the evolution of regulated proteolysis . ^^^ We have obtained values for enthalpy , entropy , and K ( d ) for binding of proteins and peptides by the nonspecific protease trypsin and the highly specific protease urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The specific induction of urokinase type plasminogen activator ( u PA ) in the damaged tissue was further confirmed by the fact that both u PA protein mass and mRNA were markedly increased in the damaged cerebral areas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This is not explained by modifications of proteases involved in cell invasiveness ( matrix metalloproteinases and urokinase type plasminogen activator ) , but by a disorganisation of actin cytoskeleton due to RhoA inhibition related to its defective prenylation as it was reversed by geranylgeraniol ( GGOH ) and mimicked by the Rho selective inhibitor C 3 exoenzyme . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : The numbers of lung metastases on the lung surface and the messenger RNA expression of MMP 9 , membrane type IBMMP , and urokinase type plasminogen activator at 24 hours after surgery were enhanced in proportion to the degree of surgical stress . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator is activated in stratum corneum after barrier disruption . ^^^ The protease was identified as urokinase type plasminogen activator , because flybrinolytic activity of the collected SC was abolished by addition of anti urokinase antibody . ^^^ These findings indicated that urokinase type plasminogen activator is activated in SC after barrier disruption and this activation might trigger the plasminogen / plasmin system in the epidermis . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the effect of HGF on the invasion of PC 3 and DU 145 prostate cancer cells through a reconstituted basement membrane ( Matrigel ) , the haptotactic migration to fibronectin substrate , the expression of protein and mRNA for matrix metalloproteinases ( MMP ) 1 and 9 , membrane type 1 MMP ( MT 1 MMP ) , urokinase type plasminogen activator ( u PA ) and its receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor specific transcription factor binding to an activator protein 2 / Sp1 element of the urokinase type plasminogen activator receptor promoter in a first large series of resected gastrointestinal cancers . ^^^ This study gives the first clinical evidence that urokinase type plasminogen activator receptor ( u PAR ) gene expression is tumor specifically regulated via an activator protein ( AP ) 2 / Sp1 promoter element in a large patient subpopulation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To quantify the ex vivo production of proangiogenic proteins ( vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( tPA ) ) and angiogenesis inhibitors ( plasminogen activator inhibitor type 1 ( PAI 1 ) and angiostatin ) from epithelial and stromal components of primary prostate cancer ( CaP ) and benign prostatic hyperplasia ( BPH ) cultures . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( u PAR ) contributes to cell migration and proteolysis in normal and cancerous tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Infections were initiated through noninvasive intratracheal administration of M . avium 724 in mice individually deficient for plasminogen , tissue type plasminogen activator , urokinase type plasminogen activator , and urokinase type plasminogen activator receptor , along with wild type control mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator system in cancer and other pathological conditions : introduction and perspective . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The structural basis of the interaction between single chain urokinase type plasminogen activator ( scuPA ) and its receptor ( uPAR ) is incompletely defined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The Quebec Platelet Disorder ( QPD ) is an unusual bleeding disorder associated with increased platelet stores of urokinase type plasminogen activator ( u PA ) and proteolysis of platelet alpha granule proteins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This depression of RhoA activity is necessary to permit a second ERK dependent signaling event via uPAR , the receptor for urokinase type plasminogen activator , to activate Rac and to drive motility through polarized lamellipodia extension . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Taken together with results of previous studies showing that beta ( 1 ) integrins were also associated with other surface receptors and proteins in chondrocytes that cause cartilage destruction in arthritis ( for example , urokinase type plasminogen activator receptor and matrix metalloproteinases ) , we can hypothesize that signal transduction by these receptor complexes via beta ( 1 ) integrins may play a crucial role in pathogenesis of osteoarticular disorders . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of Pdef also results in the down regulation of urokinase type plasminogen activator and activation of the promoter of the tumor suppressor gene , MASPIN : Growth suppressive effects of Pdef expression are mediated in part by a G ( 0 ) G ( 1 ) cell cycle arrest associated with elevated p 21 levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Clinical significance of expression of urokinase type plasminogen activator in patients with prostate cancer . ^^^ BACKGROUND : Urokinase type plasminogen activator ( u PA ) has been reported to overexpress in several types of human cancers and correlate with cancer progression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ovarian surface epithelial cells in close contact with the apical wall of preovulatory ovine follicles secrete a urokinase type plasminogen activator in response to surge levels of gonadotropins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue specimens from eight patients with failing or failed infra inguinal vein bypasses and three specimens from normal veins were harvested to study urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) by in situ hybridization and immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The down regulation of transcriptional anti activator protein 1 ( AP 1 ) activity by TAC 101 paralleled the inhibition of cell invasion and migration through the repression of expression of the mRNAs for urokinase type plasminogen activator ( u PA ) and its receptor ( u PAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here we provide evidence that PGE 2 transactivates c Met R ( contingent upon functional EGFR ) , increases tyrosine phosphorylation and nuclear accumulation of beta catenin , and induces urokinase type plasminogen activator receptor ( uPAR ) mRNA expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Soluble urokinase type plasminogen activator receptor inhibits cancer cell growth and invasion by direct urokinase independent effects on cell signaling . ^^^ The urokinase type plasminogen activator receptor ( uPAR ) is released from human cancers and is readily detected in blood . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A new synthetic matrix metalloproteinase inhibitor modulates both angiogenesis and urokinase type plasminogen activator activity . ^^^ However , this new compound also inhibited urokinase type plasminogen activator activity on fibrin zymogram , while BB 94 and BB 2516 did not . ^^^ Furthermore , the addition of urokinase type plasminogen activator reduced the inhibitory effect of the tubular morphogenesis of vascular endothelial cells by OPB 3206 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our studies ruled out the possibility that endogenously produced plasminogen activators ( i . e . tPA and urokinase type plasminogen activator ) are responsible for the LRP mediated internalization of active neuroserpin , but could not rule out the possibility that another cell associated proteases capable of binding active neuroserpin functions in this capacity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparative evaluation of urokinase type plasminogen activator receptor expression in primary breast carcinomas and on metastatic tumor cells . ^^^ The urokinase type plasminogen activator receptor ( uPAR , CD 87 ) plays a central role in the plasminogen activation cascade , which participates in extracellular matrix degradation , cell migration and invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor for urokinase type plasminogen activator regulates fibronectin matrix assembly in human skin fibroblasts . ^^^ Previous studies have indicated that the receptor for urokinase type plasminogen activator , uPAR , can form functional complexes with integrin receptors thereby modulating integrin activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( uPAR / CD87 ) is a glycosylphosphatidylinositol anchored membrane protein with multiple functions in extracellular proteolysis , cell adhesion , cell migration and proliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We show here that alpha5beta1 expression , and hence function , during angiogenesis is regulated by the transcription factor Hox D 3 , a homeobox gene that also controls the expression of endothelial cell integrin alphavbeta 3 and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The aim of this work was to evaluate by immunohistochemistry ( IHC ) the expression of both LRP 1 and urokinase type plasminogen activator receptor ( uPAR ) at different developmental stages of rat prostate disease by using a prostate cancer model previously developed in our laboratory . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Synergy of in vitro thrombolytic action of combinations of recombinant staphylokinase and single chain urokinase type plasminogen activator . ^^^ Kinetics of lysis of human plasma clots immersed in plasma were studied in vitro at 37 degrees C under the influence of recombinant staphylokinase , single chain urokinase type plasminogen activator ( scu PA ) , and their simultaneous and consecutive combinations . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Evidence for similar expression of protein C inhibitor and the urokinase type plasminogen activator system during mouse testis development . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
CD 87 ( urokinase type plasminogen activator receptor ) , function and pathology in hematological disorders : a review . ^^^ The analysis of CD 87 ( urokinase type plasminogen activator receptor uPAR ) expression has a potential role in the diagnostic or prognostic work up of several hematological malignancies , particularly acute leukemia and multiple myeloma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The glycolipid anchored urokinase type plasminogen activator receptor ( uPAR ) is engaged in various signal transduction events related to cell adhesion , migration and proliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma levels of the thrombin activation system ( thrombin antithrombin complex [ TAT ] , tissue factor pathway inhibitor [ TFPI ] , and prothrombin fragments 1+2 [ F1+2 ] ) and plasmin activation system ( tissue and urokinase type plasminogen activator [ t PA and u PA ] , plasminogen activator inhibitor 1 [ PAI 1 ] , and D dimer ) were measured with enzyme linked immunosorbent assay kits before angiography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Interleukin 1beta induced expression of the urokinase type plasminogen activator receptor and its co localization with MMPs in human articular chondrocytes . ^^^ The urokinase type plasminogen activator receptor ( uPAR ) plays a critical role in cartilage degradation during osteoarthritis as it regulates pericellular proteolysis mediated by serine proteinases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Overexpression of urokinase type plasminogen activator receptor ( uPAR ) on tumor cell surface is essential for invasion and metastasis in a variety of tumor cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
INTERVENTIONS : We characterized the expression of tissue type and urokinase type plasminogen activator ( t PA and u PA ) as well as plasminogen activator inhibitor ( PAI ) 1 and PAI 2 in human endothelial cells ( EC ) from the microvascular pulmonary circulation ( HMVEC L ) and compared it with that of EC from pulmonary artery ( HPAEC ) and umbilical vein ( HUVEC ) under baseline conditions and upon stimulation with either tumor necrosis factor alpha or lipopolysaccharide . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Detection and clinical significance of plasma urokinase type plasminogen activator and its soluble receptor in patients with multiple myeloma ] . ^^^ OBJECTIVE : To study the plasma levels of urokinase type plasminogen activator ( u PA ) and its soluble receptor ( suPAR ) in patients with multiple myeloma ( MM ) and to evaluate their clinical significance . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Single chain urokinase type plasminogen activator ( sc uPA ) is one of the key serine proteases involved in modulating cellular and extracellular matrix responses during tissue remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor ( uPAR ) and epidermal growth factor receptor ( EGFR ) are ubiquitous receptors involved in the control of a variety of cellular processes frequently found altered in cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased metastatic dissemination in human melanoma xenografts after subcurative radiation treatment : radiation induced increase in fraction of hypoxic cells and hypoxia induced up regulation of urokinase type plasminogen activator receptor . ^^^ Pimonidazole was used as a hypoxia marker , and hypoxia and urokinase type plasminogen activator receptor ( uPAR ) expression were detected by immunohistochemistry . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , some of the GPI 80 on pseudopodia of migrating neutrophils during the late phase was associated with urokinase type plasminogen activator receptor ( uPAR ) , a regulator of beta 2 integrin dependent adherence and migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Oleic acid also enhanced u PA ( urokinase type plasminogen activator ) mediated plasminogen activation over 50 fold . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We also demonstrated that the HGF stimulated stromal cell invasion was due in part to the induction of urokinase type plasminogen activator , a member of the extracellular proteolysis system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The authors also obtained preliminary data on its effect on urokinase type plasminogen activator expression at the highest dose level . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( uPAR ) is involved in cell migration and tissue remodelling , as a receptor for pro uPA , as a cell adhesion component , and in a soluble form as a chemoattractant . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using a model of standardized aerosol delivery of 1 . 8 U bleomycin / kg body weight in rabbits , we investigated the influence of repetitive nebulization of heparin or urokinase type plasminogen activator ( u PA ) on the development of lung fibrosis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we show that LRP1B , a member of LRs , modulates the migration of smooth muscle cells ( SMCs ) by increasing the degradation of membrane receptors , urokinase type plasminogen activator receptor ( uPAR ) , and platelet derived growth factor receptor ( PDGFR ) beta . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study of nasal mucosa , we investigated the presence of mRNA of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and plasminogen activator inhibitor 2 ( PAI 2 ) using reverse transcription polymerase chain reaction ( RT PCR ) and in situ hybridization , and compared these results with their localization in immunostained tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously established that polymorphonuclear leukocytes ( PMN ) from healthy individuals have the capacity to degrade fibrin via urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Serial bronchoalveolar lavage fluid ( BALF ) samples were assayed for prothrombin activation fragment ( F1+2 ) , thrombin antithrombin ( TAT ) complex , fibrinolytic activity , urokinase type plasminogen activator ( u PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) on days 1 , 4 , and 7 after VAP onset . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The glycolipid anchored receptor for urokinase type plasminogen activator ( uPAR ) is essential for cell surface associated plasminogen activation and is overexpressed at the invasive tumor stromal microenvironment in many human cancers . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of extracellular matrix degrading proteinases , such as matrix metalloproteinase 2 and urokinase type plasminogen activator , was up regulated in these cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Quantitative transcript profiling of 17 inflammation linked genes revealed a specific modulation pattern of astrocyte activation by MLK inhibition , for instance , characterized by up regulation of the anti stress factors inhibitor of apoptosis protein 2 and activated transcription factor 4 , no effect on manganese superoxide dismutase and caspase 11 , and down regulation of major inflammatory players like TNF , GM CSF , urokinase type plasminogen activator , and IL 6 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor cell motility and invasion have been linked to upregulated signaling from both the epidermal growth factor receptor ( EGFR ) and that for urokinase type plasminogen activator ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Relationship between urokinase type plasminogen activator receptor and vascular endothelial growth factor expression and metastasis of gallbladder cancer . ^^^ AIM : To investigate the relationship of urokinase type plasminogen activator receptor ( uPAR ) and vascular endothelial growth factor ( VEGF ) expression with clinical and pathological characteristics of human gallbladder cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PHBP digested alpha chain and beta chain of fibrinogen to prevent coagulation and cleaved single chain urokinase type plasminogen activator ( scuPA ) to the active hetero dimer form ( tcuPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Supernatant prostaglandin E 2 and mRNA expressions of COX 2 , vascular endothelial growth factor ( VEGF ) , urokinase type plasminogen activator ( u PA ) , u PA receptor , plasminogen activator inhibitor type 1 ( PAI 1 ) , and PAI 2 were measured . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Hyp DCHA inhibited in a dose dependent and noncompetitive manner various proteinases instrumental to extracellular matrix degradation ; the activity of leukocyte elastase was inhibited the most ( IC 50 = 3 micromol / L ) , followed by cathepsin G and urokinase type plasminogen activator , whereas that of the matrix metalloproteinases ( MMPs ) 2 and 9 showed an IC 50 > 100 micromol / L . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These in vitro findings had their in vivo correlates : urokinase type plasminogen activator , MMP 2 , and MMP 9 were detectable in both early and advanced human atherosclerotic lesions in colocalization with E LDL . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator by hepatocellular carcinoma cells enhances tumor cell proliferation , motility , invasiveness and metastatic dissemination . ^^^ Inhibition of the urokinase type plasminogen activator / plasmin system by protease inhibitors such as aprotinin ( Trasylol , Bayer ) have shown improvement in the clinical course of certain tumor types . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PAI 1 , the physiological inhibitor of tissue type and urokinase type plasminogen activator , is a unique member of the serpins as it exists in three distinct conformations : an active inhibitory conformation , a non inhibitory substrate conformation , and a non reactive latent conformation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is an important component of the plasminogen / plasmin system as it is the main inhibitor of tissue type and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Reduced beta 3 endonexin levels are associated with enhanced urokinase type plasminogen activator receptor expression in ApoE / mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator receptor was determined by quantitative RT PCR and enzyme linked immunoabsorption assay . ^^^ Expression of FGFR 4 Arg 388 in immortalized prostatic epithelial cells results in increased cell motility and invasion through Matrigel and was associated with increased expression of urokinase type plasminogen activator receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These targets include the activation of apoptosis ; suppression of growth factor expression or signalling ; downregulation of antiapoptotic proteins ; suppression of phosphatidylinositol 3 ' kinase / Akt , NF kappaB , Janus kinase signal transducer and activator of transcription and activator protein 1 signalling pathways ; and downregulation of angiogenesis through inhibition of vascular endothelial growth factor expression , cyclooxygenase 2 , matrix metalloproteinase 9 , urokinase type plasminogen activator , adhesion molecules and cyclin D 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Within the group of increased abundance , seven families of proteins were of particular interest : fatty acid binding proteins , chitinase like enzymes , cyclophilins , cathepsins , proteoglycans , urokinase type plasminogen activator receptor , and a macrophage scavenger receptor . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Binding of vitronectin to NBD P 9 PAI 1 does not affect SI but results in a 2 . 0 6 . 5 fold decrease in the limiting rate constant ( klim ) of RCL insertion for urokinase type plasminogen activator at pH 6 . 2 8 . 0 and for tissue type plasminogen activator at pH 6 . 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Moreover , AdCC cell lines expressed higher surface levels of urokinase type plasminogen activator receptor ( uPAR ) than did SCC cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activation system was also altered in tumors with a decrease of urokinase type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) and a strong increase of plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increases in cell size and shape correlate with over expression of two integrins and the urokinase type plasminogen activator receptor ( uPAR ) , which is also involved in cell adhesion and is often over expressed in metastatic cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Western blotting confirmed these enzymes to be tissue type plasminogen activator and urokinase type plasminogen activator , respectively . ^^^ High activities of tissue type plasminogen activator and urokinase type plasminogen activator were detected in the CSF of mice with eosinophilic meningitis , and correlated positively with CSF eosinophil numbers and total protein , respectively . ^^^ Immunohistochemistry demonstrated that tissue type plasminogen activator and urokinase type plasminogen activator localised in the endothelial cells of blood vessels , in blood clots and in infiltrated leukocytes . ^^^ These results suggest that tissue type plasminogen activator and urokinase type plasminogen activator may be play a role in the pathogenesis of eosinophilic meningitis of angiostrongyliasis . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Real time quantitative PCR determination of urokinase type plasminogen activator receptor ( uPAR ) expression of isolated micrometastatic cells from bone marrow of breast cancer patients . ^^^ Urokinase type plasminogen activator receptor ( uPAR ) was quantified by real time quantitative RT PCR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effect on proteases expression seems to be specific for urokinase type plasminogen activator and metalloproteinase 9 ( MMP 9 ) because no modulation in MMP 2 and MMP 3 production could be detected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using amide hydrogen exchange combined with electrospray ionization mass spectrometry , we have in this study determined the number of amide hydrogens on several peptides that become solvent inaccessible as a result of their high affinity interaction with the urokinase type plasminogen activator receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effect of down regulation of laminin receptor on urokinase type plasminogen activator expression in human bile duct carcinoma cells ] . ^^^ OBJECTIVE : To investigate the effect of antisense oligonucleotide ( AS OD ) of laminin receptor ( LNR ) on urokinase type plasminogen activator ( u PA ) expression in human bile duct carcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The MGpUK 5 cell line , transformed with a single chain urokinase type plasminogen activator ( scu PA ) minigene , generated mRNA transcripts and scu PA titers corresponding to 65 % or 86 % of the amount generated before serum free adaptation , despite significant loss of scu PA gene copies during adaptation to serum free culture . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Human adult bone marrow derived endothelial progenitors , or angioblasts , induce neovascularization of infarcted myocardium via mechanisms involving both cell surface urokinase type plasminogen activator , and interactions between beta integrins and tissue vitronectin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The transformed cells also showed a small increase in the activity of gelatin degrading matrix metalloproteinases ( MMPs ) and urokinase type plasminogen activator activities , neither of which was sensitive to the inhibitors of cathepsin L and B . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A recombinant gene coding for an antibody targeted urokinase type plasminogen activator was constructed for the purpose of enhancing the thrombolytic specificity of urokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Elevated amyloid beta protein ( Abeta 42 ) and late onset Alzheimer ' s disease are associated with single nucleotide polymorphisms in the urokinase type plasminogen activator gene . ^^^ The PLAU gene within this region encodes urokinase type plasminogen activator , which converts plasminogen to plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Leucocyte expression of the urokinase receptor [ urokinase type plasminogen activator receptor ( uPAR ) ] is regulated by inflammatory mediators . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptors ( uPARs ) , up regulated during tumor progression , associate with beta 1 integrins , localizing urokinase to sites of cell attachment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
NSO produced a concentration dependent inhibition of tissue type plasminogen activator ( t PA ) , urokinase type plasminogen activator ( u PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
DPPIV reexpression in prostate cancer cells blocks nuclear localization of bFGF , reduces bFGF levels , inhibits mitogen activated protein kinase ( MAPK ) extracellular signal regulated kinase ( ERK ) 1 / 2 activation , and decreases levels of urokinase type plasminogen activator , known downstream effectors of bFGF signaling pathway . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Involvement of the urokinase type plasminogen activator receptor in hematopoietic stem cell mobilization . ^^^ We investigated the involvement of the urokinase type plasminogen activator receptor ( uPAR ) in granulocyte colony stimulating factor ( G CSF ) induced mobilization of CD34+ hematopoietic stem cells ( HSCs ) from 16 healthy donors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma concentrations of soluble urokinase type plasminogen activator receptor are increased in patients with malaria and are associated with a poor clinical or a fatal outcome . ^^^ BACKGROUND : Blood concentrations of soluble urokinase type plasminogen activator receptor ( suPAR ) are increased in conditions with immune activation , and high concentrations of suPAR often predict a poor clinical outcome . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Based on earlier studies , we hypothesized that metastasis was governed primarily by the proangiogenic factor interleukin 8 ( IL 8 ) in D 12 tumors and by the invasive growth promoting receptor urokinase type plasminogen activator receptor ( uPAR ) in R 18 tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Four tumour markers for urinary bladder cancer tissue polypeptide antigen ( TPA ) , HER 2 / neu ( ERB B 2 ) , urokinase type plasminogen activator receptor ( uPAR ) and TP 53 mutation . ^^^ The urokinase type plasminogen activator receptor ( uPAR ) is a GPI linked single chain glycoprotein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Specific interference of urokinase type plasminogen activator receptor and matrix metalloproteinase 9 gene expression induced by double stranded RNA results in decreased invasion , tumor growth , and angiogenesis in gliomas . ^^^ We have previously demonstrated the effectiveness of adenovirus mediated expression of antisense urokinase type plasminogen activator receptor ( uPAR ) and matrix metalloproteinase 9 ( MMP 9 ) in inhibiting tumor invasion in vitro and ex vivo . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The addition of thrombin to human pulmonary artery endothelial cells resulted in an upregulation of PDGF A , tissue factor ( TF ) , ICAM 1 , and urokinase type plasminogen activator ( u PA ) , whereas TNF alpha and LPS failed to induce PDGF A . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrin specific plasminogen activators , including tissue type plasminogen activator ( t PA ) , single chain urokinase type plasminogen activator ( scu PA ) and staphylokinase ( Sak ) , preferentially activate fibrin associated plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously demonstrated that human recombinant soluble melanotransferrin ( hr sMTf ) interacts with the single chain zymogen pro urokinase type plasminogen activator ( scu PA ) and plasminogen . ^^^ In addition , in hr sMTf treated HMEC 1 , the expression of both urokinase type plasminogen activator receptor ( u PAR ) and low density lipoprotein receptor related protein ( LRP ) are down regulated . ^^^ However , fluorescence activated cell sorting analysis revealed a 25 % increase in cell surface u PAR in hr sMTf treated HMEC 1 , whereas the binding of the urokinase type plasminogen activator ( u PA ) * plasminogen activator inhibitor 1 ( PAI 1 ) complex is decreased . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor ( UPA R ; CD 87 ) is a membrane protein responsible for plasmin expression on cells facilitating cellular extravasations and tissue invasions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study investigated whether the release of urokinase type plasminogen activator receptor ( uPAR ) in whole blood cultures was affected by HIV infection . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effects of inhibition of urokinase type plasminogen activator ( u PA ) by amiloride in the cornea and tear fluid of eyes irradiated with UVB . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator receptor ( uPAR ) sustains cell migration through its capacity to promote pericellular proteolysis , regulate integrin function , and mediate chemotactic signaling in response to urokinase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activation of hepatocyte growth factor by urokinase type plasminogen activator is ionic strength dependent . ^^^ Various proteases reportedly cleave scHGF to generate the active two chain form ( HGF ) , including u PA ( urokinase type plasminogen activator ) , t PA ( tissue type plasminogen activator ) , kallikrein , Factor XIa , Factor XIIa , HGF activator and matriptase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Epidermal growth factor modulates prostate cancer cell invasiveness regulating urokinase type plasminogen activator activity . ^^^ Urokinase type plasminogen activator receptor ( uPAR ) and Epidermal Growth Factor Receptor ( EGFR ) are ubiquitous receptors involved in the control of a variety of cellular processes frequently found altered in cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of urokinase type plasminogen activator receptor ( uPAR ) in primary central nervous system neoplasms . ^^^ The cellular receptor for urokinase type plasminogen activator receptor ( uPAR ) is a member of the glycosylphosphatidylinositol ( GPI ) anchored protein family . ^^^ It is a specific cell surface receptor for its ligand , urokinase type plasminogen activator , which catalyzes the formation of plasmin from plasminogen to generate the proteolytic cascade and leads to the breakdown of the extracellular matrix . uPAR has been shown to correlate with a propensity to tumor invasion and metastasis in several types of non central nervous system tumors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A critical review of classical and newer individual molecular markers , such as hormone receptors , HER 2 , urokinase type plasminogen activator and plasminogen activator inhibitor 1 , cyclin E , topoisomerase 2 , and p 53 , was performed , and the preliminary results obtained using the new gene expression profiling technology are described along with their potential clinical implications . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here we show that HOXA 3 increases endothelial cell migration , induces angiogenesis in vivo , and leads to increased expression of the matrix metalloproteinase 14 ( MMP 14 ) and urokinase type plasminogen activator receptor ( uPAR ) genes in endothelial cells in culture and in vivo in response to injury . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the inhibitory effects of triptolide on the migration , gene and protein expression of urokinase type plasminogen activator ( u PA ) of endothelial cells ( ECs ) cultured in vitro and to elucidate the anti angiogenic mechanism of triptolide . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator , its receptor and u PA inhibitor type 1 affect in vitro growth and invasion of Kaposi ' s sarcoma and capillary endothelial cells : role of HIV Tat protein . ^^^ The urokinase type plasminogen activator ( u PA ) is chemotactic , chemoinvasive and mitogenic for KS cells and for MVEC . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Posttranscriptional regulation of urokinase type plasminogen activator receptor ( uPAR ) mRNA involves the interaction of a uPAR mRNA coding region sequence with phosphoglycerate kinase ( PGK ) , a 50 kDa uPAR mRNA binding protein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical staining of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , and urokinase type plasminogen activator were performed . ^^^ RESULTS : The depth of submucosal invasion was not significantly correlated with the expression of MMP 2 , MMP 9 , TIMP 1 , TIMP 2 , or urokinase type plasminogen activator , but the microvessel count was significantly correlated with the absolute depth of invasion ( r=0 . 312 , p < 0 . 05 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the association between beta 2 microglobulin , neopterin , serum levels of soluble urokinase type plasminogen activator receptor ( suPAR ) , CD 4 count , and plasma viremia with survival in 133 HIV 2 infected villagers and 160 controls living in rural Guinea Bissau . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Saruplase is the non glycosylated form of single chain urokinase type plasminogen activator ; it is produced by recombinant technique in Escherichia coli . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Messenger RNA levels of tissue type plasminogen activator , urokinase type plasminogen activator , and plasminogen activator inhibitor 1 were analysed using Taqman real time reverse transcriptase polymerase chain reaction and protein release by enzyme linked immunosorbent assay . ^^^ A similar pattern was observed for urokinase type plasminogen activator mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown that in adult mice with a null mutation in the urokinase type plasminogen activator receptor ( uPAR ) gene , maintained on a C57BL / 6J / 129Sv background , there is a selective loss of GABAergic interneurons in anterior cingulate and parietal cortex , with the parvalbumin expressing subpopulation preferentially affected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , expression of urokinase type plasminogen activator ( u PA mRNA ) and plasminogen activator activity ( PAA ) were examined in porcine oviducts by reverse transcription and polymerase chain reaction ( RT PCR ) and activity assays , respectively . ^^^ This study supports the suggestion that urokinase type plasminogen activator has a biological role within the porcine oviduct , especially at or near the time of fertilization . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Moreover , functional analysis by Madine Darby canine kidney cell scattering and urokinase type plasminogen activator assays demonstrated that hFab Met 1 is not an agonist to HGF / Met signaling compared with a murine intact monoclonal antibody ( MAb ) Met 5 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrinolysis requires the activation of plasminogen to plasmin , which is mediated by tissue type plasminogen activator and urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both urokinase type plasminogen activator ( u PA , 50 kDa ) and t PA ( 72 kDa ) were detected by Western blot ; they showed differences in their relative concentration between Post Ov and Pre Ov oviductal flushing . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This was also associated with a decrease in urokinase type plasminogen activator mRNA expression , decreased plasmin activity , and increased collagen 1 mRNA expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore Serp 1 has demonstrated the capacity to utilize a mammalian serine protease receptor , the urokinase type plasminogen activator receptor ( uPAR ) , to alter cellular signaling in part through the actin binding protein cytoskeletal system ( via filamin B ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Shifting the cells to nonpermissive temperature ( 39 . 5 degrees C ) induced their differentiation and a marked increase in total fibrinolytic activity due to the induction of urokinase type plasminogen activator ( u PA ) synthesis and secretion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study , we report activation of TGF beta by functional and physical co operation of the mannose 6 phosphate / insulin like growth factor 2 receptor ( CD 222 ) and the urokinase type plasminogen activator receptor ( CD 87 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OE DEP and OE UFP exposure reduced plasminogen activator inhibitor 1 ( PAI 1 ) production by the cells but did not affect the production of thrombomodulin , tissue type plasminogen activator , or urokinase type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we show that the RET / PTC1 oncogene , when exogenously expressed in primary normal human thyrocytes , induces the expression of a large set of genes involved in inflammation and tumor invasion , including those encoding chemokines ( CCL 2 , CCL 20 , CXCL 8 , and CXCL 12 ) , chemokine receptors ( CXCR 4 ) , cytokines ( IL1B , CSF 1 , GM CSF , and G CSF ) , matrix degrading enzymes ( metalloproteases and urokinase type plasminogen activator and its receptor ) , and adhesion molecules ( L selectin ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The increase in hepatic activity of urokinase type plasminogen activator was also accompanied by more activation of the hepatocyte growth factor receptor c Met . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Potential up regulated targets of note from this study include urokinase type plasminogen activator receptor , serine / threonine kinase 15 , thioredoxin reductase , and CDC 28 protein kinase 2 , as well as several others . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It has been shown previously that increased levels of urokinase type plasminogen activator receptor ( uPAR ) correlate well with higher invasive phenotype . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Aminomethyl ) cyclohexane carboxylic acid methylamide ( t AMCHA methylamide ) inhibits the physical interaction between urokinase type plasminogen activator and stratum corneum , and accelerates the recovery of barrier function . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Herpes simplex virus 1 recombinant virions exhibiting the amino terminal fragment of urokinase type plasminogen activator can enter cells via the cognate receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The activity of matrix metalloproteinases 2 and 9 and protein expressions of plasminogen activators ( urokinase type plasminogen activator and its receptor ) , which are the indicators of invasion and migration processes , were increased by nicotine but blocked by COX 2 and VEGFR inhibitors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator ( u PA ) and matrix metalloproteinases ( MMP ) have been implicated in cardiac inflammation , matrix remodeling , and wound healing after cardiac injury . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Recently , signaling events involving the urokinase type plasminogen activator receptor , which is a key regulator for the activation of hepatocyte growth factor ( HGF ) , have been implicated in modulating cortical neuron migration . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
No upregulation was observed for MMP 2 , urokinase type plasminogen activator , tissue type plasminogen activator , and TLRs 3 , 4 , and 9 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cell clone with the lowest level of DBC 1 expression showed induced expression of 26 genes including plasminogen activator inhibitor 2 ( SERPINB 5 ; 4 . 6 fold ) , heparin binding EGF like growth factor precursor ( DTR ; 4 . 2 fold ) , small proline rich protein 2B ( SPRR2B ; 3 . 6 fold ) , metallothionein 1 isoforms ( MT1B / MT1A / MT 1F ; from 2 . 9 to 3 . 2 fold ) , tissue type plasminogen activator precursor ( PLAT ; 2 . 8 fold ) and urokinase type plasminogen activator precursor ( PLAU ; 2 . 7 fold ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , PTX B inhibited the secretion of IL 6 induced , AP 1 dependent genes , including urokinase type plasminogen activator , CXCL8 / IL 8 , and CCL2 / monocyte chemotactic protein 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Multivariate analysis revealed that , after lymph node status ( P=0 . 0002 ) and urokinase type plasminogen activator ( P=0 . 004 ) , TGFbeta 1 was an independent prognostic marker for DFS ( P=0 . 005 ) in the overall population . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase type plasminogen activator and receptor form complexes that aid inflammatory cell invasion at sites of arterial injury . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Moreover , ErbB 2 mediated upregulation of urokinase type plasminogen activator receptor ( uPAR ) is reduced by either the PKCalpha inhibitor Go 6976 or the Src inhibitor PP 2 , and the combination of Go 6976 with PP 2 is superior to either agent alone in suppressing uPAR expression and cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , the expressions of molecules involved in MMP activating and inhibitory pathways ( urokinase type plasminogen activator and tissue type plasminogen activator ; TIMP 1 , TIMP 2 , and plasminogen activator inhibitor 1 ) were found to be increased after abdominal irradiation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND AND PURPOSE : This study was the first clinical trial in Taiwan of a new thrombolytic agent human tissue urokinase type plasminogen activator ( HTUPA ) in patients with acute ischemic stroke . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor plays a role in neutrophil migration during lipopolysaccharide induced peritoneal inflammation but not during Escherichia coli induced peritonitis . ^^^ BACKGROUND : Urokinase type plasminogen activator receptor ( uPAR ) is expressed on many different cells , including leukocytes . uPAR has been implicated to play a role in neutrophil migration to sites of inflammation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here , we have transfected endothelial cells with a GPI anchored thermotolerant green fluorescent protein ( ttGFP ) to show colocalization of this fluoroprobe with another marker of lipid rafts , urokinase type plasminogen activator receptor 1 . ttGFP was used to quantify the cell surface area occupied by lipid rafts and to examine the effect of various proatherogenic signals on lipid rafts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The prognostic relevance of urokinase type plasminogen activator ( u PA ) , u PA receptor ( u PAR ) , and plasminogen activator inhibitor 1 ( PAI 1 ) in gastric carcinoma was demonstrated in an independent patient series . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : Patients in group 1 were characterized by increased factor VIIa levels , increased prothrombin fragment 1+2 levels , reduced factor XIIa levels , diminished functional urokinase type plasminogen activator levels , and decreased levels of plasmin / alpha 2 plasmin inhibitor complexes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator receptor regulates a novel pathway of fibronectin matrix assembly requiring Src dependent transactivation of epidermal growth factor receptor . ^^^ Previous studies have indicated that the urokinase type plasminogen activator receptor ( uPAR ) can functionally interact with integrins thereby modulating integrin activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Simvastatin suppressed MMP 9 at both the mRNA and protein levels as well as at the urokinase type plasminogen activator protein level , resulting in the dramatic suppression of MMP 9 activity induced by tumor necrosis factor alpha . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this last decade , the structure and functions of the receptor for the urokinase type plasminogen activator have been extensively studied and characterized . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator , receptor , and inhibitor correlating with gelatinase B ( MMP 9 ) contribute to inflammation in gouty arthritis of the knee . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Decoy molecules based on PNA DNA chimeras and targeting Sp 1 transcription factors inhibit the activity of urokinase type plasminogen activator receptor ( uPAR ) promoter . ^^^ The expression levels of urokinase type plasminogen activator receptor ( uPAR ) are strongly correlated with metastatic potential in human cancer cell lines of melanoma , breast , lung , and colon . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The polycythemia rubra vera 1 gene ( PRV 1 ) , a member of the urokinase type plasminogen activator receptor superfamily , is overexpressed in granulocytes isolated from the peripheral blood of patients with polycythemia vera ( PV ) and essential thrombocythemia ( ET ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At present , antithrombotics , the urokinase type plasminogen activator system , the membrane bound membrane type 1 MMP , cathepsin L and the proteasome seem the most promising candidates as targets for anticancer strategies in early stages of cancer in combination with cytotoxic drugs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We examined the antigenic values of urokinase type plasminogen activator and plasminogen activator inhibitor Type 1 , cell counts , and levels of matrix metalloproteinase 2 and metalloproteinase 9 in 135 knee effusions from 80 patients with septic arthritis , rheumatoid arthritis , gouty arthritis , and osteoarthritis . ^^^ Urokinase type plasminogen activator and plasminogen activator inhibitor Type 1 antigenic values in effusions of septic arthritis were greater than those in effusions of aseptic arthritis . ^^^ The increases of urokinase type plasminogen activator and plasminogen activator inhibitor Type 1 antigenic values in effusions were associated with increased levels of prometalloproteinase 9 and the appearance of activated metalloproteinase 2 . ^^^ Antigenic values of urokinase type plasminogen activator also correlated with the appearance of activated metalloproteinase 9 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It has been reported that ligands of the macrophage scavenger receptor ( MSR ) induce a range of cellular responses including urokinase type plasminogen activator and the production of inflammatory cytokines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HGF activator ( HGFA ) , matriptase , urokinase type plasminogen activator and hepsin are the main factors responsible for converting pro HGF into active HGF . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To elucidate some of the links between homocysteine and vascular disease , we have evaluated the effect of the amino acid on the formation ( by kinetics studies ) , structure ( by electron microscopy ) and lysis of the fibrin network , using tissue type plasminogen activator ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : Immunohistochemistry was performed in paraffin embedded tissue specimens from 149 invasive breast carcinomas to detect the proteins ets 1 , p 53 , topoisomerase IIalpha , matrix metalloproteinase 7 ( MMP 7 ) and urokinase type plasminogen activator receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition of urokinase type plasminogen activator and modulation of field strengths by amiloride seem to be responsible for this effect . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Other characteristics include increased expression and storage of active urokinase type plasminogen activator ( u PA ) in platelets in the setting of normal to increased u PA in plasma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This diptheria toxin uPA fusion protein ( DTAT ) has the advantage over other fusion proteins of targeting malignant glioma cells and the endothelial cells of the neovasculature that express the urokinase type plasminogen activator receptor ( uPAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type plasminogen activator modulates airway eosinophil adhesion in asthma . ^^^ While the mechanism ( s ) of this process is not known , the expression of urokinase type plasminogen activator receptor ( uPAR ) has been found to modulate neutrophil adhesion and migration to inflammatory sites . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It has been recently shown that plasminogen binding to M . fermentans in the presence of the urokinase type plasminogen activator promotes the invasion of host cells by this organism . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Prognostic significance was lost when further adjusted for Her 2 / neu and urokinase type plasminogen activator ( p = 0 . 284 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast , in renal carcinoma cells that constitutively express HIF 1alpha and HIF 2alpha due to loss of VHL function , we found that high basal VEGF , glucose transporter 1 , urokinase type plasminogen activator receptor , and plasminogen activator inhibitor 1 expression was predominantly dependent on HIF 2alpha . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor ( PAI 1 ) is an important component of the plasminogen / plasmin system as it is the main inhibitor of tissue type ( t PA ) and urokinase type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor cell derived D6 . 1A stimulates angiogenic factor transcription , which includes increased matrix metalloproteinase and urokinase type plasminogen activator secretion , pronounced vascular endothelial growth factor expression in fibroblasts , vascular endothelial growth factor receptor expression , and strong D6 . 1A up regulation in sprouting endothelium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Aspirin upregulates expression of urokinase type plasminogen activator receptor ( uPAR ) gene in human colon cancer cells through AP 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Down regulation of integrin alpha M beta 2 ligand binding function by the urokinase type plasminogen activator receptor . ^^^ The cell adhesion molecule integrin alphaMbeta 2 associates with the urokinase type plasminogen activator receptor ( uPAR ) on monocytes and neutrophils . uPAR also associates with members of the beta 1 and beta 3 integrins , and it modulates the ligand binding function of these integrins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The in vitro thrombolytic and side effects of double strand urokinase type plasminogen activator ( tcu PA ) , p guanidinobenzoyl tcu PA ( GB tcu PA ) , and their combinations were compared . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of LR 11 , a member of the LDL receptor family and an enhancer of cell surface localization of urokinase type plasminogen activator receptor ( uPAR ) , is increased in cultured SMCs by treatment with PDGF BB . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Purification of c phycocyanin from Spirulina fusiformis and its effect on the induction of urokinase type plasminogen activator from calf pulmonary endothelial cells . c Phycocyanin ( c pc ) , a blue coloured , fluorescent protein was purified from blue green alga , Spirulina fusiformis and its effect on fibrinolytic system in vascular endothelial cells was investigated . ^^^ Fibrin zymographic studies indicated that c pc induced urokinase type plasminogen activator in the cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Assay indicators for the therapeutic effect include worm recovery , histopathological score of the fourth ventricle , tissue type plasminogen activator , urokinase type plasminogen activator , matrix metalloproteinase 9 , cerebrospinal fluid total protein , leukocyte counts , and proinflammatory cytokines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Severe combined immunodeficiency mice transgenic for urokinase type plasminogen activator ( uPA / SCID ) , with the liver replaced for human hepatocytes , were inoculated with virions passed in mouse and recovered from culture supernatants . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Microarray studies showed no increase in these ECM factors , rather down regulation of urokinase type plasminogen activator and matrix metalloproteinase 16 in glioblastoma cells treated with OA 5D5 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purified amino terminal fragment ( ATF ) of human urokinase plasminogen activator ( residues 1 135 ) , which is not required for activation of plasminogen , binds with high affinity to specific plasma membrane receptors on U 937 monocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BPTI , was constructed encoding a hybrid protein , which consists of the aminoterminal fragment ( ATF ) of urokinase type plasminogen activator ( u PA ) linked to bovine pancreas trypsin inhibitor ( BPTI ) , a potent inhibitor of plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since 80 % of synovial cells express urokinase plasminogen activator receptor ( uPAR ) , we investigated the inhibition of plasmin activation in a collagen induced arthritis ( CIA ) mice model , by expressing a uPA / uPAR antagonist molecule ( ATF ) fused to human serum albumin ( HSA ) to extend its serum half life . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The genes mapped include : vimentin ( VIM ) on HSA 10 and MMU 2 ; interleukin 2 receptor ( IL2R ) on HSA 10 and MMU ? ; ornithine aminotransferase ( OAT ) on HSA 10 and MMU 7 ; hexokinase 1 ( HK 1 ) on HSA 10 and MMU 10 ; retinol binding protein 3 ( RBP 3 ) on HSA 10 and MMU 14 ; plasminogen activator , urokinase type ( PLAU ) on HSA 10 and MMU 14 ; and alpha 2 adrenergic receptor ( ADRA 2 ) on HSA 10 and MMU 19 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The genes coding for glucose regulated protein , 78kDal ( GRP 78 ) , hormone sensitive lipase ( LIPE ) , plasminogen activator or urokinase ( PLAU ) , and D amino acid oxidase ( DAO ) were localized in the pig by radioactive in situ hybridization . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The differentiation inducer sodium butyrate ( NaB ) arrested growth of N . 1 ovarian carcinoma cells and repressed expression of cyclin D1 / prad1 and the invasiveness related protease plasminogen activator urokinase ( plau ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The mRNA levels of invasion related genes were evaluated based on a DNA microarray data set , and we found two gene abnormalities most relevant for the invasion of malignant germ cells : matrix metalloproteinase 9 ( MMP 9 ) and plasminogen activator , urokinase ( PLAU ) genes were up regulated in a study comparing tissue samples of TGCT and IGCN . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Carbachol ( 1 microM ) induced significant increases in the expression of cyclooxygenase ( COX ) 1 , COX 2 , IL 8 , and plasminogen activator , urokinase type ( PLAU ) to levels ranging from 1 . 3 to 3 . 1 fold of control . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Remarkably , almost one quarter of the differentially expressed genes encode proteins associated with inflammation and tissue remodelling , including members of the interferon ( IFN ) , tumour necrosis factor ( TNF ) , and transforming growth factor beta ( TGF beta ) signalling pathways as well as metalloproteinases ( MMP 1 , 9 , 13 , 23 ) , urokinase plasminogen activator ( PLAU ) , and cathepsins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A functional polymorphism within plasminogen activator urokinase ( PLAU ) is associated with Alzheimer ' s disease . ^^^ Within this region the plasminogen activator urokinase gene ( PLAU ) was considered as a reasonable candidate from its functional implication in plasmin generation , a serine protease capable of degrading beta Amyloid ( Abeta ) protein . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
At rest , heparin and Org 10172 did not influence the plasma concentrations of endogenous t PA antigen and activity , urokinase type PA ( u PA ) antigen , plasmin activatable pro urokinase ( scu PA ) , active urokinase ( tcu PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) antigen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the effect of phorbol myristate acetate ( PMA ) , dexamethasone ( Dex ) and reagents which raise intracellular cyclic AMP , on the production of plasminogen activator inhibitor type 2 ( PAI 2 ) in human promyelocytic leukemia cell line , PL 21 and on the production of urinary type plasminogen activator ( u PA ) in human pre B cell lymphoma cell line , RC K 8 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) and plasminogen activator inhibitor ( PAI 1 ) have been determined in endometrial curettings obtained from 46 subfertile women during proliferative , early or late secretory phases of the menstrual cycle . t PA activity and antigen concentrations was significantly higher ( P < 0 . 001 ) in late secretory endometrium than in proliferative or early secretory endometrium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) and plasminogen activator inhibitors ( PAI ) are elevated in late pregnancy with t PA and u PA remaining so at 6 weeks postnatal . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have recently shown that spermatozoa of various species contain both types of plasminogen activator , the tissue type ( t PA ) and the urokinase type ( u PA ) . ^^^ The identification of the type of the plasminogen activator ( t PA or u PA ) was made immunologically . ^^^ In human spermatozoa , the outer acrosomal membrane and plasma membrane contained both types of plasminogen activator ( t PA and u PA ) ; in addition , t PA antigen was measured . ^^^ In boar spermatozoa , the outer acrosomal membrane contained only t PA , whereas plasma membrane contained both types of plasminogen activator ( t PA and u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition of the endothelial cell autocrine angiotensin system , with either the angiotensin converting enzyme inhibitor lisinopril or the angiotensin 2 receptor antagonist sar 1 , ile 8 angiotensin 2 , leads to increased endothelial cell migration and urokinase like plasminogen activator ( u PA ) activity ( Bell , L . , and J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both urokinase plasminogen activator ( u PA ) and t PA activity increased in injured arteries and reached a maximum at 7 days . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Macrophage colony stimulating factor ( CSF 1 ) increases the tissue invasive potential of the CSF 1 receptor bearing lung carcinoma cell lines A 549 and Calu 1 by increasing the number of endogenously bound urokinase type plasminogen activators ( u PA ) s on these cells . ^^^ Preincubation of A 549 and Calu 1 cells with the anti u PA monoclonal antibody MPW5UK ( 25 micrograms / ml ) or with a 20 to 40 fold stoichiometric excess of fluid phase type 2 plasminogen activator inhibitor abrogates invasiveness , indicating that functionally active cell surface u PA is essential for tissue invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This enhancing effect of plasminogen was markedly suppressed by adding anti t PA IgG and plasmin inhibitors into the gel matrix , but less affected by anti urokinase type PA ( u PA ) IgG , offering more evidence to the hypothesis that the activation of the fibrinolytic system by PAs plays an important role in the invasiveness of murine melanoma B 16 cell lines , and indicating that t PA contributed more than u PA to the invasive potential of these cells into the pericellular matrix . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have now studied whether the initiating factor of the fibrinolytic system , urokinase plasminogen activator ( u PA ) , may also function in the early steps of activation of one of the metalloproteinases , the M ( r ) 72 , 000 gelatinase / type 4 collagenase produced by cultured fibroblasts . ^^^ On the contrary , the tissue activator of plasminogen , t PA , did not cleave the type 4 collagenase in similar assays . u PA catalyzed cleavage of recombinant type 4 collagenase , produced in a baculovirus expression system , yielded a similar M ( r ) 62 , 000 activity in gelatinolysis assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma levels of plasminogen activators ( t PA , u PA ) and their inhibitor ( PAI 1 ) were studied in patients suffering from Buerger ' s disease and healthy volunteers before and after 15 minutes of venous occlusion test . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A comparison of the plasminogen activator activity units of urinary type plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) . ^^^ The earliest tissue plasminogen activator ( t PA ) preparations prepared from melanoma cells were expressed in urinary type plasminogen activator ( u PA ) units of activity using the u PA International Standard ( 66 / 46 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 2 ( PAI 2 ) is an inhibitor of u PA and is present in several neoplastic cell lines and malignant ascites . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma concentration of thrombin antithrombin 3 complex ( TAT ) , tissue type plasminogen activator ( t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , PAI 2 , D dimer complex and urokinase plasminogen activator ( u PA ) activity were studied in 30 patients with acute nonlymphoblastic leukemia ( ANLL ) , before and during antileukemic therapy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The latter was associated with elevated FXII and PKK , while C 1 INH was decreased , ATIII and alpha 2M were unchanged ; it could not be accounted for by changes in t PA , u PA , PAI , plasminogen , alpha 2 AP , proteins C or S . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These data provide further biochemical evidence for the proteolytic cleavage of fibronectin by plasminogen activators and substantiate that u PA digestion of Fn may be an initial event in the local degradation of the extracellular matrix by malignant cells , possessing elevated levels of these enzymes . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The hormonal regulation of two plasminogen activators , tissue type plasminogen activator ( t PA ) and urokinase ( u PA ) , was studied both in 7 , 12 dimethylbenz [ a ] anthracene ( DMBA ) induced rat mammary carcinoma and in DMBA induced rat mammary dysplasia . t PA activity in DMBA mammary carcinoma was decreased markedly by oophorectomy and recovered upon estradiol administration to reach the maximum level at 12 hr . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since nidogen is suggested to be an important bridging molecule in the maintenance of the supramolecular organization in basement membranes , the presence of a binding site for u PA in nidogen indicates a role for plasminogen activation in basement membrane remodelling . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have previously shown that alpha thrombin exerted a mitogenic effect on human glomerular epithelial cells and stimulated the synthesis of urokinase type ( u PA ) and tissue type plasminogen activator ( t PA ) and of their inhibitor , plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The analysis of normal human plasma by fibrin autography revealed four species of plasminogen activator ( PA ) activity related to tissue type PA , factor 12 , prekallikrein and urokinase type PA ( u PA ) . ^^^ The dextran sulfate dependent activation of u PA required both factor 12 and prekallikrein , but did not require either plasminogen or factor 11 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of urinary type plasminogen activator ( u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , and PAI 2 were measured in gastric cancer tissues and adjacent healthy mucosal tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The goal of the present study was to assess the relative importance of receptor bound and secreted plasminogen activator urokinase ( u PA ) in generating cell surface plasmin and fostering destruction of normal tissue by tumor cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Thrombin cleaves single chain urokinase ( scu PA ) , causing its inactivation , but not two chain u PA [ tcu plasminogen activator ( PA ) ] or tissue type PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the principal inhibitor of the two activators of the fibrinolytic system : tissue and urokinase type PAs ( t PA and u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zymographic analysis of secreted PA related compounds revealed production of free urokinase ( u PA ) and type 1 plasminogen activator inhibitor ( PAI 1 ) complexed to tissular plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The respective roles of urokinase plasminogen activator ( u PA ) and the u PA receptor in extracellular matrix degradation was investigated . ^^^ Although pro u PA producing cells alone degrade the matrix in the presence of plasminogen , u PA receptor producing cells do not . ^^^ The u PA receptor must be cell associated , as binding of pro u PA to a receptor solubilized from the cell surface with phosphatidyl inositol specific phospholipase C did not enhance the activation of pro u PA in the presence of plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The authors examined the effect of insulin like growth factor 1 ( IGF 1 ) , epidermal growth factor ( EGF ) , and acidic fibroblast growth factor ( AFGF ) on the synthesis by human retinal endothelial cell ( HREC ) of plasminogen activators ( PA ; tissue type [ t PA ] and urokinase type [ u PA ] ) and plasminogen activator inhibitor ( PAI ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) , the fast acting inhibitor of tissue type plasminogen activator ( t PA ) and urokinase ( u PA ) , is a member of the serpin superfamily of proteins . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present in vitro study investigated dose , time and temperature dependent effects of two chain urokinase plasminogen activator ( u PA , urokinase ) on normal citrated plasma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) , an essential regulatory protein of the fibrinolytic system , harbors interaction sites for plasminogen activators ( tissue type [ t PA ] and urokinase type [ u PA ] ) and for fibrin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the fast acting inhibitor of both tissue type and urokinase type plasminogen activators ( t PA , u PA ) and is an essential regulatory protein of the fibrinolytic system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrinolytic system components like tissue plasminogen activators ( t PA ) , and urokinase ( u PA ) , and their inhibitors PAI 1 and PAI 2 were all studied . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The antigen levels of plasminogen activators ( PAs ) , tissue type PA ( t PA ) and urokinase type PA ( u PA ) , were measured in extracts from 30 gastric carcinomas and corresponding normal gastric mucosa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although the spontaneous dissolution of emboli was observed during the infusion of saline , tissue plasminogen activator ( t PA ) as well as urokinase plasminogen activator ( u PA ) produced a further dissolution . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators t PA , u PA and its inhibitor ( PAI ) in normal males and females . ^^^ We determined the plasma levels of tissue plasminogen activator ( t PA ) , plasminogen activator inhibitor ( PAI ) activity and their antigen levels including urokinase plasminogen activator ( u PA ) in 33 male and 27 female normal subjects . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Evidence has recently been presented that activated macrophages ( M phi ) express both urinary ( u PA ) and tissue type ( t PA ) plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine whether a relationship exists among urokinase plasminogen activator ( u PA ) activity , tissue plasminogen activator ( t PA ) activity , and the malignant transformation of human fibroblasts , we measured receptor bound and secreted u PAs and t PA activity in fibroblast cell strains of a unique cell lineage and compared the results with the values obtained in human fibrosarcoma derived cell lines and control cell lines . ^^^ The data suggest that the malignant state of human fibroblasts is always associated with high levels of activity of receptor bound u PA , and in addition cells transformed to the malignant state are very likely to exhibit high levels of receptor bound t PA and secreted forms of plasminogen activators . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We analyzed the effect of IL 1 on the synthesis of collagenase , stromelysin , and tissue inhibitor of metalloproteases ( TIMP ) in human cartilage explants and culture chondrocytes , as well as its effect on the secretion of plasminogen activators ( t PA , u PA ) and inhibitors ( PAI 1 , PAI 2 ) in cartilage explants . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The activation of native human plasminogen ( Glu 1 Pg ) by tissue plasminogen activator , urinary plasminogen activator ( u PA ) , and streptokinase is inhibited by the divalent cations Ca2+ , Mg2+ , and Mn2+ . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By the combined use of zymographies on tissue sections and in situ hybridizations , we have explored the cellular distribution of urokinase type ( u PA ) and tissue type ( t PA ) plasminogen activators and of their mRNAs in developing and adult mouse kidneys . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators ( PA ) , particularly the lower Mr urokinase ( u PA ) type , have been associated with tumor cell invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
On the other hand , when [ 125I ] urokinase plasminogen activator [ ( 125I ] u PA ) was administered intravenously to DIC rats , many silver grains were observed on MARGM of the proximal tubules in the kidney but not seen on MARGMs of the glomeruli in the kidney , nor in the lung , liver , and spleen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type ( u PA ) and tissue type plasminogen activator antigen ( t PA ) as well as plasminogen activator inhibitor activity were determined in seminal plasma and lysates of the respective spermatozoas in 67 ejaculate of males in infertile marriage without genito urinary pathology . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have determined in vivo the relative antithrombotic efficacy and hemostatic safety of combining low dose activated protein C ( APC ) and urokinase ( urinary plasminogen activator , u PA ) , two natural proteins that regulate thrombogenesis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fast acting inhibitors of both urokinase ( u PA ) and tissue plasminogen activator ( two chain t PA ) were noted in harvest fluids of Hep G 2 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using site directed mutagenesis , we have changed the asparagine in human single chain urinary plasminogen activator ( u PA ) at position 302 to an alanine . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In 22 human tumor cell lines the regulation of production of plasminogen activators urokinase ( u PA ) and tissue type ( t PA ) and their inhibitors PAI 1 and PAI 2 was studied . ^^^ The frequent finding of t PA ( alone or in combination with u PA ) suggests that t PA can also be a tumor associated plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In order to investigate this question , we studied human renal biopsies by immunofluorescence technique with specific antibodies for fibrin , tissue type plasminogen activator ( t PA ) , urokinase ( u PA ) , PAI 1 and PAI 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 1 ( PAI 1 ) was identified in extracts of Lewis lung carcinoma , and its immunohistochemical localization was studied together with that of urokinase type ( u PA ) and tissue type ( t PA ) plasminogen activators . ^^^ The results are consistent with an assumption that the plasminogen activation system , and particularly u PA and PAI 1 , plays a role in regulation of breakdown of extracellular matrix proteins during invasive growth in this carcinoma . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We now report the synthesis and secretion of PA by rat alveolar epithelial cells with the catalytic properties of a urokinase type ( u PA ) rather than tissue type plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Todd ' s autohistographic method , modified by Lotti , was used to investigate the FA and the monoclonal antibodies against u PA and t PA were used to identify the main plasminogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The thrombolytic effects of tissue type of plasminogen activator ( t PA ) and urokinase type of plasminogen activator ( u PA ) were studied in experimental rabbit model of pulmonary thromboemboli with or without endotoxin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A collaborative study to establish a standard for high molecular weight urinary type plasminogen activator ( HMW / u PA ) . ^^^ An international collaborative study involving eleven laboratories located in eight countries was undertaken to establish an International Standard for high molecular weight urinary type plasminogen activator ( HMW / u PA ) . ^^^ The current International Reference Preparation ( IRP code numbered 66 / 46 ) for urinary type plasminogen activator ( u PA ) or urokinase ( see Nomenclature footnote ) is a 66 / 34 molar ratio mixture of low molecular weight ( LMW ) and high molecular weight ( HMW ) u PA ' s and is considered unsuitable as a standard for homogeneous preparations of HMW / u PA . ^^^ The putative standard for HMW / u PA ( code number , 87 / 594 ) was compared for potency in a clot lysis assay with the current IRP for u PA ( code number , 66 / 46 ) and a lyophilised preparation of single chain urinary type plasminogen activator ( SCuPA ) , the latter being used in the assay without prior activation by plasmin to its active two chain form ( TCuPA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The binding affinity ; incorporation and adsorption , of human tissue plasminogen activator ( t PA ) and urokinase plasminogen activator ( u PA ) for blood clots was investigated in vitro . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The localization of tissue plasminogen activator ( t PA ) or urokinase plasminogen activator ( u PA ) on thrombi was investigated in disseminated intravascular coagulation rats ( DIC rats ) induced by thrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A number of hybrid plasminogen activator genes were constructed from the t PA and u PA cDNAs and expressed using a bovine papilloma virus vector and mouse C 127 cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cultured human endothelial cells synthesize and secrete two types of plasminogen activator , tissue plasminogen activator ( t PA ) and urokinase ( u PA ) . ^^^ The results suggest that in addition to sharing a matrix associated binding site ( plasminogen activator inhibitor type 1 ) , both t PA and u PA have unique membrane binding sites which may regulate their function . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and urokinase ( u PA ) are proteins with partial structural similarity and which are of importance in the therapy of thrombotic diseases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The increased propensity to form tumors did not correlate with the expression of urinary or tissue plasminogen activators ( u PA or t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In addition , several forms of the plasminogen activator urokinase ( u PA ) , which shares partial structural homology with t PA , were evaluated as competitors of cellular binding . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This inhibition was correlated with an increase in PAI 1 antigen expression , whereas urinary type plasminogen activator ( u PA ) secretion was unaffected . ^^^ Coculture experiments demonstrated that LPAI cells prevented matrix degradation by Lu PA cells ( L cells expressing high levels of u PA ) or Co 115 human colon carcinoma cells ( expressing tissue type plasminogen activator ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TPA treated K 562 cells also synthesize and secrete platelet derived growth factor ( PDGF ) , transforming growth factor beta 1 ( TGF beta 1 ) , urokinase plasminogen activator ( u PA ) and its specific inhibitor , type 1 plasminogen activator inhibitor ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urine Plasminogen activator ( U PA ) activity was measured by the fibrin plate method in 73 patients with various degrees of renal function deterioration from various underlying diseases and in one healthy individual in order to evaluate the plasminogen activator activity of remnant nephrons . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These proteases fall into two classes : serine proteases include plasminogen activators ( t PA ) and urokinase ( u PA ) and play a major role in fibrinolysis , tissue repair and carcinogenesis ; and metalloproteases include collagenases and stromelysine , two enzymes involved in the tissue remodelling that occurs during angiogenesis and tumor growth . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Abbokinase is a commercially available two chain lower molecular weight urinary plasminogen activator ( u PA ) with low affinity for fibrin . ^^^ Therefore , therapeutic use of this u PA form ( UK ) may cause activation of not only clot bound plasminogen but also systemic plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase ( u PA ) was mixed with increasing amounts of monocyte extract for 10 min and the mixtures were added to 125I fibrin coated wells containing plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
During pregnancy , the t PA and prourokinase ( u PA ) antigen concentrations increased 50 % and 200 % , respectively , whereas the plasminogen and alpha 2 antiplasmin levels remained constant . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To assess in vivo the postulated participation of urokinase type ( u PA ) and tissue type ( t PA ) plasminogen activators in processes involving tissue remodeling and cell migration , we have studied the cellular distribution of u PA and t PA mRNAs during mouse oogenesis and embryo implantation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma concentrations of tissue type plasminogen activator ( t PA ) , urokinase ( u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and PAI 2 were studied in 53 patients with liver deficiency caused by chronic alcoholism ( n = 40 ) , viral hepatitis ( n = 10 ) or malignant disease of the liver ( n = 3 ) and compared to that of a control group ( n = 20 ) of healthy subjects . u PA and PAI 1 levels were significantly increased in all patients with chronic alcoholism , whereas high t PA was only observed in combination with disturbed liver function tests or with liver cirrhosis ( two and six fold above control values , respectively ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Functional determination of tissue type ( t PA ) or urinary type ( u PA ) plasminogen activator , the key enzymes of fibrinolysis , is of clinical importance . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The properties of porcine urokinase plasminogen activator ( u PA ) , produced and secreted by Saccharomyces cerevisiae , were studied to evaluate processing of the enzyme by yeast . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The PAA was due to the tissue type plasminogen activator ( t PA ) , but not to the urokinase type ( u PA ) , as judged by addition of respective antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Macrophages have a marked capacity to invade tissue in the course of cellular immune reactions that is thought to be based on the action of urokinase ( u PA ) . u PA is an ubiquitous serine protease that converts the zymogen plasminogen into the active protease plasmin . u PA binds to specific receptors on the macrophage thereby enabling the cell to degrade interstitial tissue in the microenvironment . ^^^ This conclusion is further supported by the findings that : 1 ) saturation of monocytes with u PA does not further increase their invasiveness and that 2 ) plasminogen activator inhibitor 2 , a specific inhibitor of u PA associated with endogenously occupied , but not of u PA bound to saturable receptors , inhibits monocyte invasiveness completely . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
SDS PAGE in the presence of casein and plasminogen is consistent with the major PA species of urinary type ( u PA ) and with the absence of PA inhibitor in the parent and revertant clones . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They were specifically bound to 125I t PA but not 125I urokinase ( u PA ) and inhibited t PA , but not u PA , activity in plasminogen rich 125I fibrin wells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma concentrations of urokinase plasminogen activator ( u PA / competitive radioimmunoassay ) , tissue type plasminogen activator ( t PA / sandwich ELISA ) and plasminogen activator inhibitor ( PAI / functional assay ) were determined in 44 women ( age : 24 . 3 + / 4 . 3 years ) with normal pregnancy near term . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
They synthesize and secrete tissue type plasminogen activators ( t PA ) , urinary type plasminogen activators ( u PA ) , as well as plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Culture of human mammary HBL 100 cells in the presence of dexamethasone , a synthetic glucocorticoid , resulted in opposite effects on the production of the two plasminogen activators ( PAs ) : a decrease in urokinase type PA ( u PA ) and a concomitant increase in tissue type PA ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two types of plasminogen activator ( PA ) , t PA ( tissue type ) and u PA ( urokinase type ) , are released from endometrial tissue in organ culture , as judged by immunological identification and molecular weight . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In women undergoing conization of the uterine cervix , peripheral blood samples and blood samples from the cone cavity were obtained and analysed for their concentration of plasminogen activator of tissue type ( t PA ) and of urokinase type ( u PA ) and the concentration of fibrinogen / fibrin degradation products ( FDP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
There are two distinct forms of PA : urokinase ( u PA ) and tissue type plasminogen activator ( t PA ) . t PA has higher affinity for fibrin and is the main form involved in thrombolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Rapid release and deactivation of plasminogen activators in human endothelial cell cultures in the presence of thrombin and ionophore A 23187 . alpha Thrombin , DFP thrombin , and ionophore A 23187 induce the rapid release ( less than 5 minutes ) of a variety of proteins , including t PA forms ( Mr 110 and 70 k , after SDS PAGE ) from primary cultures , and both t PA and u PA ( Mr 90 and 54 k ) from subcultured human HUVECs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Rat adrenal glands were stained immunocytochemically using antibodies against plasminogen activators of the tissue type ( t PA ) and urokinase type ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biopsies of involved and uninvolved skin from psoriatic patients and of normal skin were stained immunocytochemically with monoclonal antibodies against urokinase type ( u PA ) and tissue type ( t PA ) plasminogen activator using a multilayer peroxidase technique . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Assays for tissue like plasminogen activator ( t PA ) and urokinase like plasminogen activator ( u PA ) in cell conditioned medium revealed an increased appearance of both enzymes shortly after the S phase of the cycle . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Comparison of the mobility of the plasminogen activator species on SDS polyacrylamide gel electrophoresis with human urokinase ( u PA ) and human melanoma tissue type plasminogen activator ( t PA ) and studies with antibodies to these enzymes indicate that the Mr approximately 50 , 000 species is a u PA and the Mr approximately 65 , 000 a t PA . ^^^ Abolition of plasminogen activator activity in the fibrin plate assay with antibodies to t PA and u PA also confirms enhanced t PA production on interleukin 1 stimulation , though there is also evidence for increased cell associated production of u PA . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase type ( u PA ) and tissue type ( t PA ) plasminogen activators are decreased in the culture media of TGF beta treated cells concomitantly with the increase in PAI 1 accumulation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma levels of t PA antigen , t PA activity , u PA antigen and plasminogen activator inhibitor were determined by means of immunological and functional assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue plasminogen activator ( t PA ) and urokinase ( u PA ) , the major activators of plasminogen , are synthesized and released from endothelial cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The distribution of tissue plasminogen activator ( t PA ) and urokinase ( u PA ) was investigated immunohistochemically in squamous epithelium of the uterine cervix , in normal and dysplastic conditions as well as in preinvasive and invasive carcinomas . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Four fibrinolytic parameters , i . e . fibrinolytic activity at rest ( FAr ) , fibrinolytic response after 10 minutes of venous occlusion ( delta FA 10 ' ) , and resting antigenic levels of the plasmatic inhibitor of the plasminogen activators ( PAI 1 ) and of urokinase ( u PA ) , showed significant differences between the two study groups . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Constitutive gene expression of four components of plasminogen activating enzyme system , urinary and tissue type plasminogen activator ( u PA and t PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) and PAI 2 in HT 1080 human fibrosarcoma cells , was modulated by the synthetic glucocorticoid dexamethasone ( Dex , 10 ( 7 ) M ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase and tissue type plasminogen activators ( u PA and t PA ) were identified immunohistochemically during reepithelialization of mouse and human skin wounds , by means of polyclonal and monoclonal antibodies . ^^^ The function of the plasminogen activators during reepithelialization is discussed and it is suggested that the keratinocytes use plasmin activated by u PA for dissecting their way through the provisional matrix in the upper part of the granulation tissue . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The results of the present study on the kinetics of u PA activity in cultured macrophages , its subcellular localization and the effect of an organophosphorus ester are consistent with the concept that the development of pericellular proteolysis proceeds through a series of stages , namely , ( a ) synthesis of pro u PA , ( b ) binding to membrane receptors , ( c ) activation to a double chain u PA , and ( d ) conversion of plasminogen into plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Artificial exon shuffling between tissue type plasminogen activator ( t PA ) and urokinase ( u PA ) : a comparative study on the fibrinolytic properties of t PA / u PA hybrid proteins . ^^^ We constructed two human tissue type plasminogen activator / urokinase ( t PA / u PA ) hybrid cDNAs which were expressed by transfection of mouse Ltk cells . ^^^ Plasminogen activation by either one of the hybrid proteins in the absence of a fibrin substitute was similar to that by HMW u PA , while rt PA was much less active . ^^^ The hybrid molecules form a 1 : 1 molar complex with the human endothelial plasminogen activator inhibitor ( PAI 1 ) , analogous to that formed by rt PA and HMW u PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our knowledge about the components of the fibrinolytic system has greatly increased during the last few years thanks to the cloning of the cDNA of the two plasminogen activators t PA and u PA , of the two plasminogen activator inhibitors PAI 1 and 2 , and of other profibrinolytic and of inhibitory factors of this system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Breast cyst fluids obtained by needle aspiration from 20 patients were analysed for the presence of urokinase like ( u PA ) and tissue type ( t PA ) plasminogen activators . ^^^ Plasminogen , the natural substrate for these PAs , was also measured . u PA activity was detected in 18 samples ( mean + / S . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The following reaction conditions were found optimal : 50 microliter of undiluted plasma , anticoagulated with citrate or EDTA , were first incubated with 1 IU u PA in a Tris buffer , pH 8 . 4 and then with Glu plasminogen ( 0 . 85 mumol / l final ) , CT ( 2 . 5 mmol / l final ) , tranexamic acid ( 0 . 9 mmol / l final ) for 5 min . at 37 degrees C . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The regulation of urokinase ( u PA ) and tissue plasminogen activator ( t PA ) in cultured normal and neoplastic urothelium was examined because plasminogen activators ( PAs ) are thought to be important in malignancy . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The following tests were performed : euglobulin clot lysis time ( ECLT ) , fibrinolytic activity of euglobulins on fibrin plates in the presence and absence of blocking antibodies to tissue type plasminogen activator ( t PA ) and / or urokinase ( u PA ) , overall plasminogen activator inhibitor ( PAI ) activity , antigen levels of t PA , u PA and PAI 1 and zymography of the euglobulin fraction after SDS PAGE . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The current investigation involved the secretion of plasminogen activators ( PA ) of tissue type ( t PA ) or urokinase type ( U PA ) by testing supernatants of 21 permanent human leukemic cell lines originating from various hematopoietic lineages and one induced lymphoblastoid cell line . ( LCL ) The amount of PA in each supernatant was determined by biologic and immunoenzymologic assays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two hybrid plasminogen activators , plasmin A chain / t PA B chain and plasmin A chain / u PA B chain have been synthesized and purified in sufficient yield to permit measurement of clearance in small laboratory animals . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator ( PA ) activity , in particular urokinase ( u PA ) , has been shown to be markedly increased in adenocarcinomas of the colon . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The cells secreted tissue type plasminogen activator ( t PA ) and PA inhibitor 1 , and , after subculturing , urokinase type PA ( u PA ) antigen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators in alveolar bone in man was studied in quenching experiments by a fibrin slide technique with addition of monospecific antibodies against tissue plasminogen activators ( t PA ) and urokinase ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
It formed complexes with urokinase ( u PA ) and with plasminogen activator of the tissue type ( t PA ) , similar to those formed by the placental plasminogen activator inhibitor ( PI PA 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activation is mediated by plasminogen activators , which are classified as either tissue type plasminogen activators ( t PA ) or urokinase type plasminogen activators ( u PA ) . ^^^ Two physiological plasminogen activators , t PA and single chain u PA ( scu PA ) induce clot specific thrombolysis , via entirely different mechanisms , however . t PA is relatively inactive in the absence of fibrin , but fibrin strikingly enhances the activation rate of plasminogen by t PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrinolysis induced by human tumor cells producing either urokinase ( u PA ) or tissue type plasminogen activators ( t PA ) was inhibited by growth in vitro of the tumor cells together with aortic bovine smooth muscle cells ( SMC ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A plasminogen activator inhibitor ( PA 1 ) which inhibits primarily plasminogen activator of the urokinase type ( u PA ) was isolated from the cytosol of human peripheral leukocytes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We immunocytochemically stained rat pituitary glands using antibodies against plasminogen activators of the tissue type ( t PA ) and the urokinase type ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The fibrinolytic activity of these cells results from the production of both a tissue type plasminogen activator ( t PA ) and a urokinase like activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Serum free medium conditioned by SMC neutralized both tissue type ( t PA ) and urokinase like ( u PA ) plasminogen activators . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Monoclonal antibodies to tissue type plasminogen activator ( t PA ) , urokinase ( u PA ) and alpha 2 antiplasmin were obtained by immunizing Balb C mice with the respective purified antigens and fusion of spleen cells with mouse myeloma cells ( NSO ) . ^^^ Only one out of 5 monoclonal antibodies directed against u PA inhibited plasminogen activation by u PA . ^^^ None of the monoclonal antibodies directed against t PA or u PA inhibited the cleavage of low molecular weight paranitroanilide substrates by the respective plasminogen activators . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , the relative contribution of the two known types of plasminogen activators , urokinase type ( u PA ) and tissue type ( t PA ) , was evaluated using specific antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The major species of plasminogen activator in the harvest medium of the stimulated cultures resembled u PA when analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by fibrin zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inhibition studies with antibodies directed against human urokinase ( u PA ) or tissue plasminogen activator ( t PA ) revealed the presence of a plasminogen activator completely inhibitable by anti t PA IgG in the vessel walls , while in collecting ducts the plasminogen activator activity was inhibited by anti u PA IgG to only 80 % and by anti t PA IgG to about 20 % . ^^^ In carcinomatous prostate glands total fibrinolytic activity was significantly higher , but localization and relative contribution of anti u PA inhibitable and anti t PA inhibitable plasminogen activators remained the same . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We now report that purified human tissue type plasminogen activator ( t PA ) , but not urokinase ( u PA ) , has a similar TEF activity , at doses as low as 2 ng / ml ( 30 pM ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen , plasminogen activator inhibitor 1 ( PAI 1 ) and 2 ( PAI 2 ) , tissue type plasminogen activator ( t PA ) , urokinase ( u PA ) and D dimers ( D D ) were quantified in plasma samples and pleural effusion specimens . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The thrombi , adjacent vein wall , and distant veins ( the superior vena cava ) were removed at intervals from 1 hour to 21 days from formation and then cryohomogenized and assayed with specific bioimmunoassays for tissue type ( t PA ) and urokinase type plasminogen activators ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Biochemical and histochemical studies of plasminogen activator of urokinase type ( u PA ) activity . 1 . ^^^ A simple rapid fluorescent method for the detection of plasminogen activator activity of urokinase type ( u PA ) in the tear fluid is described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This study investigated the contribution of urokinase plasminogen activator ( u PA ) to hyperfibrinolysis and the effects of high dose perioperative aprotinin ( Trasylol ) on fibrinolytic activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Specific binding of urinary type plasminogen activator ( u PA ) to vitronectin and its role in mediating u PA dependent adhesion of U 937 cells . ^^^ The present paper described interactions of urinary type plasminogen activator ( u PA ) with isolated protein components of the extracellular matrix ( ECM ) using kinetic and ligand blotting analyses , as well as adhesion studies with u PA saturated U 937 monocytic cells . ^^^ Kinetic analyses showed that fibronectin and laminin were moderately effective at decreasing activation of plasminogen by u PA ( 3 4 fold decrease in kcat / Km ) , while activation was stimulated slightly by collagen types 1 and 4 ( 2 4 fold increase in kcat / Km ) . ^^^ Plasminogen activator inhibitor type 1 did not compete with u PA for binding to vitronectin , suggesting both molecules may co localize on vitronectin . ^^^ In contrast , binding of u PA to vitronectin was significantly inhibited by plasminogen , suggesting these molecules share a common binding site on vitronectin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The low density lipoprotein receptor related protein / alpha 2 macroglobulin receptor ( LRP ) mediates endocytosis of a number of structurally unrelated ligands , including complexes of plasminogen activator inhibitor type 1 ( PAI 1 ) and tissue type plasminogen activator ( t PA ) or urokinase plasminogen activator ( u PA ) , free t PA , single chain urokinase ( pro u PA ) , alpha 2 macroglobulin protease complexes , and lipoprotein lipase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although procoagulant molecules ( e . g . , tissue factor [ TF ] ) and fibrinolytic components ( e . g . , urokinase [ u PA ] and type 1 plasminogen activator inhibitor [ PAI 1 ] ) have been detected in alveolar fluid from injured lungs , the origin of these molecules remains unknown . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase and tissue type plasminogen activators ( u PA and t PA ) in normal and neoplastic tissues of cervix and of vulva were immunohistochemically identified by means of polycyclonal antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Retinal pigment epithelial cell surface u PAR was assayed by measuring the amount of functional urokinase plasminogen activator ( u PA ) bound to cells at saturation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We report that in addition to the transcriptional induction of PAI 2 , treatment of SCC 12F cells with 10 nM TCDD also resulted in an increase in urokinase plasminogen activator ( u PA ) mRNA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The purified receptor was found to bind urokinase type plasminogen activator type 1 plasminogen activator inhibitor complex ( u PA . ^^^ PAI 1 ) , while there was no or very weak binding of active site blocked u PA ( DFP u PA ) , PAI 1 or u PA type 2 plasminogen activator inhibitor complex . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Progesterone stimulates degradation of urokinase plasminogen activator ( u PA ) in endometrial stromal cells by increasing its inhibitor and surface expression of the u PA receptor . ^^^ Activation of plasminogen by receptor bound u PA initiates a cascade of proteolytic events in the extracellular matrix that is important during tissue proliferation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrin degradation is plasmin dependent and is regulated by the balance between plasminogen activators ( PA ) , tissue PA ( t PA ) , urokinase PA ( u PA ) , and their inhibitors ( PAI ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
FGF 2 internalization occurring through HSPGs was paralleled by an increase in the activity of urokinase plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , TSV PA lacks the sequence site that has been demonstrated to be responsible for the interaction of t PA ( KHRR ) and u PA ( RRHR ) with plasminogen activator inhibitor type 1 . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Methods for measuring antigen and activity of plasminogen activators ( t PA , u PA ) , plasminogen activator inhibitors ( PAI 1 , PAI 2 ) and their complex have been improved in the past few years , but few comparative data are available and they should be standardized . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase ( u PA ) is expressed in stromal cells and only in tumour cells at invasive foci , urokinase receptor ( u PAR ) in tumour cells , plasminogen activator inhibitor type 1 ( PAI 1 ) in the intratumoral extracellular matrix and plasminogen activator inhibitor type 2 ( PAI 2 ) in tumour cells and stromal cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study the expression of tissue type plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) and plasminogen activator inhibitors 1 and 2 ( PAI 1 and PAI 2 ) antigen in conditioned medium from cultured human umbilical vein ( HUVEC ) and human saphenous vein ( HSVEC ) endothelial cells was investigated under basal conditions and after stimulation with LPS , TNF alpha , interferon gamma ( IFN gamma ) or interleukin 6 ( IL 6 ) alone or in combinations . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We report binding of plasminogen , tissue type plasminogen activator ( t PA ) and urinary type plasminogen activator ( u PA ) to intact SMCECM with concentrations of ligand yielding half maximal binding ( B 50 ) of 34 , 5 and 15 nM , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The activation of collagen bound plasminogen by cellular urokinase like plasminogen activator ( u PA ) was 60 % more efficient than the activation of the soluble ( not bound ) form of plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of the plasminogen activators u PA and t PA as well as the inhibitor PAI 1 were studied immunohistochemically in 35 osteosarcoma specimens compared to 15 Ewing ' s sarcomas and various other bone lesions . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Using various goat immunoglobulin G ( IgG ) containing antibodies ( anti human uterine tissue type PA ( t PA ) , anti human low molecular weight ( LMW ) urokinase , and nonspecific goat IgG ) and plasminogen free fibrin agarose plates , we confirmed that the cholesteatoma tissue extracts contained 72 kd t PA and 64 kd urokinase type PA ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase ( u PA ) mediated cell surface plasminogen activation is required for cellular tissue invasion . ^^^ This invasion occurs in environments rich in plasminogen activator inhibitors ( PAIs ) , which efficiently inhibit receptor bound two chain u PA . ^^^ Single chain u PA ( scu PA ) was recently found to efficiently initiate cell surface plasminogen activation , and we herein describe the interaction of scu PA with PAI type 2 ( PAI 2 ) . ^^^ These data suggest that the initiation of cell surface plasminogen activation may involve the partitioning of scu PA between PAI 2 ( a `` negative modulator ' ' ) and the u PA receptor ( a `` positive modulator ' ' ) and that the enzymatic activity of receptor bound scu PA may allow initiation of cell surface proteolysis even in PAI 2 rich environments . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The conversion of plasminogen into serine protease plasmin with fibrinolytic activity depends on the actual balance between plasminogen activators ( urokinase type ; u PA and tissue type ; t PA ) and their inhibitors ( type 1 and 2 plasminogen activator inhibitors ; PAI 1 and PAI 2 ) . ^^^ Cancer cells strongly labelled for both u PA and t PA , but epithelial cells of fibroadenoma samples were also stained for plasminogen activators at least as intensively as tumour cells in cancerous tissues . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( u PA ) receptor ( u PAR ) is a glycosyl phosphatidylinositol anchored membrane protein that promotes pericellular proteolysis and cellular migration . ^^^ Due to decreased u PA binding , SPE B treated U 937 cells expressed decreased activity against a u PA specific fluorogenic substrate and plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We quantitated urokinase and tissue plasminogen activator ( u PA , t PA ) , plasminogen activator inhibitor 1 and 2 ( PAI 1 , PAI 2 ) , and fibrinolytic activity in peripheral blood ( PB ) , tumour blood ( TB ) , peritoneal / ascitic fluid ( PAF ) and cystic fluid ( CF ) from 104 patients with benign and 36 patients with malignant ovarian tumours , and in peripheral blood from 62 healthy controls . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effects of heparan sulfate analogue or other sulfated polysaccharides on the activation of plasminogen by t PA or u PA . ^^^ Activation rate of native plasminogen ( Glu plg ) by tissue plasminogen activator ( t PA ) , urinary plasminogen activator ( u PA ) and single chain u PA ( scu PA ) was enhanced in the presence of HHS 5 dose dependently up to 10 micrograms / ml . ^^^ The enhancement of the activation of plasminogen by t PA or u PA was more significant in the presence of HHS 5 than in the presence of chondroitin sulfate C , dextran sulfate or heparin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study we have verified the mitogenic effect of urokinase type ( u PA ) and tissue type plasminogen activators ( t PA ) on human normal fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator type was determined by anti human t PA and urokinase ( u PA ) antibody blocking before activity assay ( n = 7 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The B 16 cell lines did not secrete any gelatinase , but they secreted TIMP 2 , tissue type ( t PA ) , urokinase type ( u PA ) plasminogen activators and PAI 1 like activities . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
TNF alpha enhances urokinase plasminogen activator ( u PA ) activity and steady state mRNA levels twofold without affecting tissue plasminogen activator ( t PA ) or PAI 1 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , we measured the antigen levels of urokinase ( u PA ) , tissue plasminogen activator ( t PA ) , type 1 plasminogen activator inhibitor ( PAI 1 ) , and type 2 plasminogen activator inhibitor ( PAI 2 ) , and cancer tissue ( 19 adenocarcinomas and 19 squamous cell carcinomas ) and normal lung tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urinary type plasminogen activator ( u PA ) production was one order of magnitude greater than production of tissue type plasminogen activator ( t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( u PA ) is a serine protease which plays an important part in the regulation of plasmin production from plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Indirect evidence suggests a crucial role for the fibrinolytic system and its physiological triggers , tissue type ( t PA ) and urokinase type ( u PA ) plasminogen activator , in many proteolytic processes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Here we report that mast cell tryptase is a potent activator of single chain urinary type plasminogen activator ( scu PA , or prourokinase ) , the zymogen form of urinary type plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma levels of urokinase type ( u PA Ag ) and tissue type ( t PA Ag ) plasminogen activator are both enhanced during physical exercise . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Potential activators of pro stromelysin 1 , plasmin , and u PA ( urinary plasminogen activator ) , were also detected in conditioned media of Fn f treated cartilage . u PA levels were increased in the presence of the Fn f but by only a few fold . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of plasminogen activators ( PA ) , tissue type ( t PA ) and urokinase type ( u PA ) , as well as PA inhibitors ( PAI ) , type 1 ( PAI 1 ) and type 2 ( PAI 2 ) , were investigated immunohistochemically in 97 human pancreatic carcinomas . u PA expression predominated in pancreatic carcinomas , compared with t PA [ u PA expression in 76 specimens ( 78 . 4 % ) and t PA in eight specimens ( 8 . 2 % ) ] . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor type 2 ( PAI 2 ) is an inhibitor of u PA and is present in several neoplastic cell lines and malignant ascites . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine the levels of free plasminogen activator activity in human aqueous humor and to identify the type of activity ( i . e . , tissue type t PA or urokinase type u PA ) that is responsible . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In pre eclampsia ( PE ) , reduced levels of plasma urokinase like plasminogen activator ( u PA ) and plasminogen activator inhibitor 2 ( PAI 2 ) , and increased levels of plasma tissue type plasminogen activator ( t PA ) antigen were seen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We studied the plasminogen activation system in tumor tissue by measuring the antigen level of the 2 plasminogen activators , tissue type ( t PA ) and urokinase type ( U PA ) and their inhibitors , plasminogen activator inhibitors type 1 ( PAI 1 ) and type 2 ( PAI 2 ) in the tissue extracts of 43 human benign and malignant ovarian tumors . ^^^ These results were integrated in a plasminogen activation dependent malignancy index ( U PA 10 PAI I / t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
HT 29 cells express u PA receptors ( 100 , 000 sites / cell , KD = 1 . 5 nM ) , but no PA activity and therefore are unable to generate plasmin in the presence of plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ala 44 Lys50 , Ala 44 Glu51 and Lys 19 Lys20 . enhanced the activation of Glu plg by tissue plasminogen activator ( t PA ) or urinary plasminogen activator ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both plasminogen ( Pg ) and urinary type Pg activator ( u PA ) , but not tissue type Pg activator ( t PA ) , bind to normal and rheumatoid arthritis ( RA ) human synovial fibroblasts in culture with high affinity and in a dose dependent manner . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The pharmacokinetic and thrombolytic properties were determined of two recombinant single chain chimeric plasminogen activators ( PA ) consisting of u PA 33k , a low molecular weight derivative of single chain urokinase type PA ( scu PA ) comprising amino acids Ala 132 through Leu 411 , and of either a single chain variable region fragment ( Fv ) derived from the fibrin fragment D dimer specific monoclonal antibody MA 15C5 ( K12G0S32 ) or of the deglycosylated single chain Fv fragment obtained by substitution of Asn 88 with Glu ( K12G2S32 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the present study we found that , in normal cell culture conditions in 10 % FCS the plasminogen activation cascade works continuously on monocytoid leukemia cells , which expressed plasmin activity and active u PA on their cell surface . ^^^ This plasmin catalyzed the conversion of the produced pro u PA to active 2 chain urokinase ( tcu PA ) , and was derived from bovine serum plasminogen by the activity of cell bound tcu PA . ^^^ Plasmin generation was abolished and pro u PA accumulated in cell cultures that were grown for several days , either in the presence of serum thoroughly depleted of plasminogen , or in the presence of 1 mM tranexamic acid . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator ( PA ) is a serine protease which exists in two forms : tissue type ( t PA ) and urokinase type ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the absence of accessory components , plasminogen activator inhibitor 1 ( PAI 1 ) rapidly forms equimolar , inactive complexes both with tissue type ( t PA ) and with urokinase type ( u PA ) plamsinogen activator . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Venous blood samples were taken at time 0 , 3 , 6 , 25 and 49 h for assay of fibrinogen ( Fbg ) , fibrinopeptide A ( FPA ) , total fibrin ( ogen ) degradation products ( TDP ) , fibrin degradation products ( FbDP ) , fibrinogen degradation products ( FgDP ) , cross linked fibrin degradation products ( XL FDP ) , tissue plasminogen activator ( tPA ) , urinary type plasminogen activator ( u PA ) , plasminogen , alpha 2 antiplasmin ( alpha 2 AP ) and plasminogen activator inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasma is incubated with either streptokinase , urokinase ( u PA ) , tissue plasminogen activator ( t PA ) or plasminogen streptokinase activator complex ( PSAC ) at room temperature . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type and urokinase type plasminogen activators ( t PA and u PA ) coexist in numerous biological fluids , where their fibrinolytic activities are determined by the concentration of the free , uncomplexed species . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator activity ( PAA ) and plasminogen activator inhibition ( PAI ) , against t PA ( t PAI ) or u PA ( u PAI ) , in spermatozoa and seminal plasma as well as testosterone in the blood of Friesland , Chios , and Karagouniki rams all showed a seasonal variation with the highest values during the corresponding breeding season of the ewes ( Autumn Winter ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These water soluble cyclopeptides behave as time dependent inhibitors of bovine trypsin and human urokinase ( u PA ) but have no effect on tissue plasminogen activator ( t PA ) and no or poor effect on plasmin and thrombin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Lysis of blood clots from vehicle and retinoic acid treated rats could be completely blocked by addition of tranexamic acid or antibodies against rat t PA before clot formation . t PA activity in plasma was slightly increased after retinoic acid treatment ; no effects were measured on plasma PAI 1 , u PA , plasminogen , and alpha 2 antiplasmin levels . t PA activity in lung and kidney was marginally enhanced by retinoic acid but in heart and aortic tissue extracts t PA activity was increased by about 50 % . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Whereas plasminogen activator of the tissue type ( t PA ) is present in extracts of kidney parenchyma , only small amounts of the enzyme can be detected in normal urine where the major plasminogen activator is of the urokinase type ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Analysis of canine PPP , WBS , platelet lysates , and primary canine endothelial cell ( EC ) cultures by sodium dodecyl sulfate polyacrylamide gel electrophoresis and fibrin autography revealed a plasminogen dependent lytic zone at 45 kd relative molecular mass that was shown to be related to urokinase type PA ( u PA ) by its selective inhibition through amiloride . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The former which cleaved plasminogen directly and produces plasmin , includes urokinase type PA , tissue type PA , single chain u PA , bat PA , mutant PA , hybrid PA , IgG carrying PA and bispecific monoclonal antibody . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We , therefore , performed immunohistological studies on human colon tumours using monoclonal antibodies against urokinase ( u PA ) and tissue type plasminogen activator ( t PA ) as well as against plasminogen activator inhibitors 1 and 2 ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase plasminogen activator ( u PA ) and its inhibitor ( PAI 1 ) in embryo fetal bone formation in the human : an immunohistochemical study . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The balance of proteases and inhibitors differed dramatically from that observed in plasma , with higher levels of t PA , PAI 1 and PAI 2 , and lower levels of u PA ( urokinase ) , plasminogen , alpha 2 AP and t PA PAI 1 complex in bone marrow , and resulted in favourable conditions for fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The expression of tissue type plasminogen activator ( t PA ) , urokinase type PA ( u PA ) and PA inhibitor 1 ( PAI 1 ) in such treated cells was investigated by specific ELISAs on the protein level and by Northern blotting on the mRNA level . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We investigated the effects of IL 1 on the production of tissue type plasminogen activator ( t PA ) , urokinase ( u PA ) and PAI 1 by glomerular cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Urokinase plasminogen activator ( u PA ) plays a key role in the metastatic process by promoting plasmin mediated tissue degradation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Direct and reverse fibrin zymography indicated the presence of urokinase like plasminogen activator ( u PA ) and PAI 1 in U 251 conditioned media and cell lysates . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Since PAI 1 inhibits the 2 major plasminogen activators , tissue type plasminogen activator ( t PA ) and urinary type plasminogen activator ( u PA ) in an equimolar manner it was important to establish the potency of the PAI 1 inhibitor in terms of both t PA and u PA neutralisation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : In 50 consecutive node positive breast cancer patients , serial coagulation studies ( fibrinogen method of Clauss , antithrombin 3 , protein C amidolytic methods , D dimer enzyme linked immunoadsorbent assay [ ELISA ] techniques , and plasminogen activator inhibitor [ PAI ] activity u PA inhibition test ) and impedance plethysmography ( IPG ) for screening of deep vein thrombosis ( DVT ) were performed preoperatively and postoperatively , before each of six cycles of adjuvant chemotherapy ( 60 mg / m2 epirubicin and 600 mg / m2 cyclophosphamide ) and 3 months thereafter . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The enhanced fibrinolytic potential was attributed to increased urokinase plasminogen activator ( u PA ) , probably released from perturbed endothelial cells by IPC . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A potential activator of pro Sln 1 , urokinase ( u PA ) , was released at elevated levels in the presence of the Fn f while other activators , tissue plasminogen activator ( t PA ) and plasmin activities were not detected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This exceptional property of t PA , however , is not shared by urokinase ( u PA ) , a plasminogen activator that is very closely related to t PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULTS : LIF increased the accumulation of urokinase plasminogen activator ( u PA ) in the Hep G 2 cell culture supernatants determined by enzyme linked immunosorbent assay ( ELISA ) ( 0 . 21 + / 0 . 03 ( SE ) ng / ml at baseline ; 0 . 40 + / 0 . 05 ng / ml at 100 U / ml , P < 0 . 05 ; 0 . 57 + / 0 . 06 ng / ml at 500 U / ml , P < 0 . 01 ; n = 9 ) without altering total protein content . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In AAA tissue , elevated levels of both urokinase type and tissue type plasminogen activators ( u PA and t PA ) have been documented . u PA and t PA have been localized to macrophages within the inflammatory infiltrate which is characteristic of AAA . mRNA expression of both type PAs is elevated as well in comparison to both normal and atherosclerotic occlusive aorta . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this study , the expression of three glycoproteins that play a role in the plasminogen activator system as activators of proteolysis urokinase type plasminogen activator ( u PA ) , tissue type plasminogen activator ( t PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) were studied in various components of dedifferentiated chondrosarcomas of bone . ^^^ CONCLUSIONS : The current study documents the overexpression of u PA , t PA , and PAI 1 in dedifferentiated chondrosarcoma and suggests involvement of the plasminogen activator system in the biology of these tumors . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Zymographic analysis of plasminogen activator ( u PA ) revealed an inhibition of u PA secretion in MC transfected with c src 295 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The T cell lines were found to express either urokinase ( u PA ) and high levels of u PA receptor or tissue type plasminogen activator ( t PA ) and low levels of u PA receptor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Incubation with transforming growth factor beta 1 resulted in a decrease in plasminogen activation , primarily because of an increase in plasminogen activator inhibitor 1 RNA and protein and a decrease in u PA RNA as noted by quantitative reverse transcriptase polymerase chain reaction , Western analysis , and zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both plasminogen and plasminogen activators ( t PA and u PA ) bind to specific cellular receptors ; assembly of components of the fibrinolytic system at the endothelial cell surface results in stimulation of fibrinolytic activity . ^^^ Several mechanisms contribute to this stimulation , eg , enhanced plasminogen activation by t PA or u PA , enhanced conversion of scu PA to tcu PA , and impaired inhibition of plasmin by alpha 2 antiplasmin or of PAs by PAIs . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
This PAI 1 was partly co localized with macrophages , as was the urokinase plasminogen activator ( u PA ) , suggesting an involvement of these cells in peritoneal tissue fibrinolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
CONCLUSION : The inhibition of cartilage degradation by glucocorticosteroids may be due to down regulation of urokinase plasminogen activator ( u PA ) activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Amilorid , previously known as a diuretic , is found to be a competitive inhibitor of plasminogen activator urokinase ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The receptor ( u PAR ) for urokinase plasminogen activator ( u PA ) is a three domain protein , GPI anchored to the cell surface , which focuses the enzymatic activity of u PA , and allows the cell surface activation of plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In previous studies we found that colorectal carcinomas have a marked increase of the urokinase type of plasminogen activator ( u PA ) , and the inhibitors PAI 1 and PAI 2 , whereas the tissue type plasminogen activator ( t PA ) is found to be decreased in comparison with adjacent normal mucosa . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Several clones expressing mutant u PA and luciferase were identified by Western blotting , plasminogen zymography , and reverse transcription PCR . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase receptor ( u PAR ) , a protein anchored to cell membrane by a glycosyl phosphatidylinositol , plays a central role in cancer cell invasion and metastasis by binding urokinase plasminogen activator ( u PA ) , thereby facilitating plasminogen activation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Quantitative reverse transcription polymerase chain reaction and in situ hybridization were employed to investigate the expression of tissue type and urokinase type plasminogen activators ( t PA and u PA , respectively ) , of their specific inhibitor ( PAI 1 ) , and of the procoagulant molecule tissue factor ( TF ) in tissues from mice that develop autoimmune disease ( MRL lpr / lpr ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : The authors analyzed the secretion of extracellular matrix degrading proteinases , including urinary type plasminogen activator ( u PA ) , matrix metalloproteinase 2 ( MMP 2 , gelatinase A ) , and MMP 9 ( gelatinase B ) , by short term primary cultures of epithelial ovarian carcinoma cells derived from primary ovarian tumors , intraperitoneal metastases , or ascites . ^^^ METHODS : A coupled spectrophotometric assay was utilized to evaluate the initial rate of plasminogen activation by u PA in conditioned medium ; this involved monitoring the activity of generated plasmin with a colorimetric substrate . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To define the role of the plasminogen activators ( PAs ) tissue PA ( t PA ) and urokinase PA ( u PA ) in vascular wound healing , neointima formation and reendothelialization were evaluated after electric or mechanical arterial injury in mice with a single or combined deficiency of t PA ( t PA / ) and / or u PA ( u PA / ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and urokinase plasminogen activator ( u PA ) influence persistence of luminal thrombi and proteolysis of extracellular matrix , respectively . ^^^ The major physiologic inhibitor of t PA and u PA is plasminogen activator inhibitor type 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cultured fibroblasts from patients with systemic sclerosis ( SSc ) and normal individuals were examined for gene expression of types 1 and 3 collagen , decorin , matrix metalloproteinases ( MMP ) MMP 1 , MMP 2 , and MMP 3 , tissue inhibitors of metalloproteinases ( TIMP ) TIMP 1 and TIMP 2 , urokinase and tissue type plasminogen activators ( u PA and t PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , Sertoli cell tryptase expression as well as urokinase plasminogen activator ( u PA , also a serine protease ) increased significantly when germ cells were adhering to Sertoli cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of the urokinase ( u PA ) and tissue type plasminogen activator ( t PA ) related fibrinolysis in their urine was followed using sensitive and specific assays , and the changes related to post operative blood loss . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue and urokinase plasminogen activators ( t PA , u PA ) and their inhibitor PAI 1 in blood of patients with laryngeal neoplasms ] . ^^^ In this study we measured the concentrations of tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) , activity of plasminogen activator inhibitor type 1 ( PAI 1 ) and euglobulin lysis time ( ELT ) in the blood of 20 men aged 42 75 years old with planoepitheliale larynx carcinoma , and 10 healthy persons in similar age . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the acute phase , intense plasminogen activator inhibitor 1 ( PAI 1 ) gene expression was apparent in areas interfacing the dissecting hematoma , but no tissue type PA ( t PA ) , urokinase type PA ( u PA ) , or MMP 9 mRNAs were detected . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Physiological concentration of factor XIIa was as more efficient than soluble u PA to lyse fibrin as a result of activation of Glu plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Although systemic tissue plasminogen activator ( t PA ) and urokinase like plasminogen activator ( u PA ) levels showed no significant differences from normal adults , enhanced fibrinolysis was indicated by elevated D dimer and low plasminogen levels in the neonates in this study . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tissue type plasminogen activator ( t PA ) and urokinase like plasminogen activator ( u PA ) activities and antigen levels have been determined in polyp tissue and control nasal mucosa . t PA activity is higher in nasal mucosa ( median : 4 . 26 i . u . / mg ) as compared with nasal polyps ( median : 0 . 65 i . u . / mg ; p = 0 . 03 ) ; u PA activity is slightly lower in nasal mucosa ( median : 0 . 040 i . u . / mg ) as compared to polyps ( median : 0 . 065 i . u . / mg ; not significant ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : Definition of the changes in the activators of plasminogen , u PA and t PA , and examination of the possible generation of plasmin in the circulation in overwhelming sepsis . ^^^ MEASUREMENT AND RESULT : t PA , PAI 1 , t PA PAI 1 complexes , plasminogen , fibrinogen and plasmin alpha 2 antiplasmin complexes were measured serially by ELISA and free u PA by SDS PAGE with zymography . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Discordant expression of mRNA and protein for urokinase and tissue plasminogen activators ( u PA , t PA ) in endometrial carcinoma . ^^^ Urokinase and tissue plasminogen activators ( u PA , t PA ) were assayed in acetate detergent buffer extracts and their mRNAs quantitated in autoradiograms of Northern blots . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We applied monoclonal antibodies against MMP 1 , 2 , 3 , 9 and their inhibitors TIMP 1 / 2 , as well as against urokinase plasminogen activator u PA and its inhibitor PAI to investigate their influence on articular cartilage degradation in patients with varusgonarthritis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Conditioned medium concentrations of urokinase plasminogen activator ( u PA ) were increased by TGFbeta 1 , but decreased by estradiol + progesterone . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
IL 1 ( 2 ng / mL ) specifically increased the accumulation of PAI 1 ( 4 . 4 + / 0 . 6 fold ; mean + / SD ; n = 9 ) without affecting tissue plasminogen activator ( t PA ) or urokinase plasminogen activator ( u PA ) levels , which remained unchanged . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cell motility is accompanied by a rapid decrease in the cytoskeleton bound E cadherin , an acceleration of cellular adhesion to the substrate , an up regulation of urokinase plasminogen activator ( u PA ) RNA and protein , and a change in the solubility and proteolysis of the u PA receptor . ^^^ Cell motility is significantly reduced by inhibitors of u PA proteolytic activity such as antibodies neutralizing u PA activity , plasminogen activator inhibitor 1 , and amiloride . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator , urokinase ( u PA ) , interacts with the u PA receptor ( u PAR ) which results in enhanced plasminogen activation on cell surfaces . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
BACKGROUND : Arterial aneurysms exhibit a loss of elastin and an increase in the plasminogen activators urokinase plasminogen activator ( u PA ) and tissue plasminogen activator ( t PA ) . ^^^ Because u PA , t PA , and plasmin have a limited proteolytic activity against elastin , the role of plasminogen activators in the aneurysmal disease is unclear . ^^^ To investigate this question , we overexpressed plasminogen activator inhibitor 1 ( PAI 1 ) , an inhibitor of t PA and u PA , in a rat model of aortic aneurysm . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Abnormal expression of fibrinolytic genes [ e . g . , tissue type and urokinase type plasminogen activators ( t PA and u PA ) and their specific inhibitor ( PAI 1 ) ] and of the procoagulant molecule tissue factor ( TF ) , has been reported in various types of renal diseases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The following haemostatic parameters were determined ; thrombelastography , fibrinogen , antithrombin 3 ( ATIII ) , thrombin antithrombin ( TAT ) complex , beta thromboglobulin ( beta TG ) , plasminogen activators ( t PA , u PA ) , plasminogen activator inhibitors ( PAI 1 , PAI 2 ) , and plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Synoviocytes from osteoarthritis and rheumatoid arthritis produce plasminogen activators and plasminogen activator inhibitor 1 and display u PA receptors on their surface . ^^^ The presence of urokinase Plasminogen Activator ( u PA ) receptors ( u PAR ) on the surface of synovial cells was investigated by radio ligand binding assay and cross linking and by transmission electron microscopy ( TEM ) of a gold u PA complex . ^^^ Our results showed a low production of tissue type Plasminogen Activator ( t PA ) in both OA and RA SF , but relatively high levels of u PA , until confluence , both in OA and in RA . ^^^ Our data show that SF in vitro produce mainly u PA , the most important plasminogen activator involved in tissue modifications . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Endothelial cells express fibrinolytic proteins including : urokinase ( u PA ) and tissue type ( t PA ) plasminogen activators , type 1 ( PAI 1 ) and 2 ( PAI 2 ) plasminogen activator inhibitors , and u PA receptor ( u PAR ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of urinary type plasminogen activator ( u PA ) activity and tissue inhibitor of matrix metalloproteinase ( TIMP ) 1 was observed in all 4 cell lines . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The secretion of plasminogen activator ( u PA ) and the capacity of DU 145 cells to migrate through a Matrigel coated membrane were significantly inhibited in the presence of micromolar concentrations of SDA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
RESULT ( S ) : The concentrations of urokinase plasminogen activator ( u PA ) and PAI 1 were higher in endometrium from women with endometriosis than in endometrium from controls and even higher in endometriotic tissue than in endometrium from both groups . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Expression of the plasminogen activators ( PA ) , urokinase type PA ( u PA ) and tissue type PA ( t PA ) , and expression of the matrix metalloproteinases ( MMPs ) , MMP 3 , MMP 9 , MMP 12 , and MMP 13 , were significantly increased within 15 d of transplantation when cells actively migrate . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
High tissue content of urokinase plasminogen activator ( u PA ) is associated with high stromal expression of u PA mRNA in poorly differentiated serous ovarian carcinoma . ^^^ Urokinase plasminogen activator ( u PA ) plays a pivotal role in tissue degradation during tumor spread and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator / U PA / in blood of patients with lung cancer ] . ^^^ Clinical and experimental studies suggest that urokinase plasminogen activator / u PA / is associated with cancer invasion and metastasis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Ets 1 is a member of the ets gene family of transcription factors , which binds to the ets binding motif in the cis acting elements and regulates the expression of certain genes such as proteases including urokinase plasminogen activator ( u PA ) and matrix metalloproteinase 1 ( MMP 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator receptors ( u PAR ) were measured by radioligand binding , cell cross linking , immunoassay , and RNAse protection assay . u PA and plasminogen activator inhibitors ( PAIs ) expression and activities were analyzed by zymography , immunoassay , and RNase protection assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : The expression patterns of urokinasase plasminogen activator ( u PA ) , its cell surface receptor ( u PAR ) , and cathepsin B were analyzed by immunohistochemical techniques in 11 cases of parosteal osteosarcoma and in 4 cases of dedifferentiated parosteal osteosarcoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the multistep process of tumor metastasis , different cellular functions are known to be influenced by the urokinase plasminogen activator ( u PA ) . ^^^ The antisense transfection resulted in : ( 1 ) stable incorporation of the vector construct into cellular genome , as demonstrated in Southern blot ; ( 2 ) decreased u PA expression in Northern blot ; ( 3 ) 50 % reduced u PA protein expression in both cell homogenate and cell culture medium ; ( 4 ) unchanged cellular proliferation and u PAR ( urokinase plasminogen activator receptor ) expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To estimate the individual role of the plasminogen activators ( PA ) urokinase ( u PA ) and tissue ( t PA ) in the development of two renal diseases ( the nephrotic forms of chronic glomerulonephritis ( CGN ) and amyloidosis , the baseline plasma and urine levels of u PA and t PA antigens , their functional activity ( FPAA ) , and changes in these parameters were determined after protein loading test ( 0 . 7 g / kg ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Monocyte plasminogen activator inhibitor 2 ( PAI 2 ) inhibits u PA mediated fibrin clot lysis and is cross linked to fibrin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A cell line , TKM 33 , has been established and cloned from human umbilical vein endothelial cells , was previously reported to produce a large amount of urokinase type PA ( u PA ) and small amounts of tissue type plasminogen activator ( t PA ) and PA inhibitor 1 ( PAI 1 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The activation of ubiquitous plasminogen by urokinase ( u PA ) and tissue type plasminogen activator ( t PA ) , which is associated with various neuropathologies , including multiple sclerosis ( MS ) , is the key initiator of the activation cascade of the four classes of matrix metalloproteinases ( MMPs ) : collagenases , stromelysins , membrane type metalloproteinases and gelatinases . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine the ability of radiation to modulate mesangial cell expression of various molecules involved in promoting extracellular matrix ( ECM ) accumulation [ fibronectin , plasminogen activator inhibitor 1 ( Pai 1 ) , and tissue inhibitor of metalloproteinase 2 ( Timp 2 ) ] and degradation ( Tgfb , plasminogen activators u PA or t PA , matrix metalloproteinases Mmp 2 and Mmp 9 ) , primary cultures of rat mesangial cells ( passage number 6 11 ) were placed in serum free medium 24 h prior to irradiation with single doses of 0 . 5 20 Gy ( 137 ) Cs gamma rays . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The appearance and possible role of plasminogen activator of urokinase type ( u PA ) activity in the cornea related to soft contact lens wear in rabbits . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Migration through Transwell inserts in vitro in the presence of plasminogen was increased up to 22 % by overexpression of u PA , whereas t PA had no effect . ^^^ The migratory and metastatic phenotype of HT 1080 tumor cells is thus directly dependent on u PA expression levels and can be altered by gene transfer of u PA or plasminogen activator inhibitors . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The following parameters were determined : plasminogen activators ( t PA , u PA ) , plasminogen activator inhibitor 1 ( PAI 1 ) , D dimer , beta thromboglobulin ( beta TG ) , thrombin antithrombin ( TAT ) complex , fibrinogen , Factor 7 , platelets , haematocrit and haemoglobin levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Stromelysin 1 ( MMP 3 ) cleaves a 55 kDa kringle 1 4 fragment , containing the lysine binding site ( s ) involved in cellular binding , from 92 kDa plasminogen and removes a 17 kDa NH 2 terminal fragment , containing the cellular receptor binding site , from 45 kDa urokinase ( u PA ) , but a potential role of MMP 3 in the regulation of cellular fibrinolytic activity by affecting binding and / or activation of plasminogen and / or single chain u PA has not been established . ^^^ Treatment with MMP 3 ( final concentration 0 50 nM ) of cells saturated with bound plasminogen ( about 25 nM ) , overnight at 37 degrees C , resulted in a dose dependent reduction of the amount of u PA activatable plasminogen ( reduction to 25 40 % of the value in the absence of MMP 3 ) . ^^^ Treatment with MMP 3 ( final concentration 0 25 nM ) of cell bound u PA ( about 17 nM for THP 1 and 47 nM for HT 1080 cells ) , overnight at 37 degrees C , did not alter cell associated u PA activity , measured in a direct chromogenic substrate assay or in a plasminogen coupled chromogenic substrate assay ( residual u PA activity always > or =85 % of that without MMP 3 treatment ) . ^^^ These data indicate that MMP 3 may downregulate cell associated plasmin activity by decreasing the amount of activatible plasminogen , without affecting cell bound u PA activity . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The present morphological study was therefore designed to investigate the occurrence and distribution of the tissue and urokinase plasminogen activators ( t PA and u PA ) , the u PA receptor ( u PAR ) and the plasminogen activator inhibitors ( PAI 1 and PAI 2 ) in normal human testis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator ( u PA ) and its fast acting type 1 inhibitor ( PAI 1 ) localize to cellular focal adhesive structures and the adjoining proximal undersurface region , respectively ( Kutz et al . , J . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Two relatively simple electrochemical assay methods suitable for the measurement of plasminogen activators ( including urokinase ( u PA ) , streptokinase ( SK ) , and tissue plasminogen activator ( t PA ) ) in plasma samples are described . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The increase in matrix accumulation was associated with little or no increases in the proteinases , urokinase plasminogen activator ( u PA ) and transin , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen , plasminogen activator inhibitor ( PAI ) 1 , tissue type plasminogen activator ( t PA ) and urokinase plasminogen activator ( u PA ) were investigated in the pre surgical period and in the postoperative follow up period in children suffering from Ewing sarcoma ( ES ; n = 36 ) or osteosarcoma ( OS ; n = 39 ) . ^^^ Besides a short lasting increase of PAI 1 in patients with OS on day 1 and in children with Es on day 14 , a small and significant but clinically irrelevant difference was found on days 7 10 for plasminogen , t PA and u PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators u PA ( urokinase ) and t PA ( tissue ) as well as the inhibitors PAI 1 and PAI 2 are present in gingival crevicular fluid in concentrations significantly greater than in plasma . ^^^ The present study describes , by means of in situ hybridization and immunohistochemistry , the localization of the plasminogen activators and their inhibitors in gingival tissues from patients undergoing periodontal surgery . t PA mRNA and t PA antigen were primarily found in the epithelial tissues , predominantly in the sulcular and junctional regions , although occasionally in the oral epithelium and in blood vessels of the connective tissue . u PA and u PA receptor signals were seen in single cells within the junctional and sulcular epithelia and adjacent to blood vessels close to the junctional epithelium , but rarely in the oral epithelium . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A fragment of recombinant urokinase plasminogen activator ( u PA ) , was expressed in E . coli in the form of inclusion bodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this investigation , we show that the serine protease urokinase plasminogen activator ( u PA ) , which is causally involved in metastasis , is expressed at lower levels in BCCs compared to other skin cancers , such as squamous cell carcinomas ( SCCs ) or malignant melanomas . ^^^ In contrast to u PA , tissue plasminogen activator , which is not thought to be involved in metastasis , was present at similar levels in the different types of skin lesion investigated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Decreased t PA antigen was seen at 18 months and decreased urokinaselike plasminogen activator ( u PA ) antigen occurred throughout the 24 months of both OCs use . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The concentrations of tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) in cell conditioned medium were determined by ELISA and the level of PAI 1 mRNA was determined using northern hybridisation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : To determine the ability of radiation to modulate kidney tubule epithelial cell expression of various molecules involved in regulating extracellular matrix accumulation ( collagen types 1 and 3 , fibronectin , plasminogen activator inhibitor 1 ( PAI 1 ) , TGF beta and tissue inhibitor of metalloproteinases 2 ( TIMP 2 ) ) and degradation ( plasminogen activators u PA or t PA , MMP 2 and MMP 9 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Activity of plasminogen activator inhibitor ( PAI 1 ) , platelet adhesion and aggregation , antigens of tissue and urokinase plasminogen activators ( respectively , t PA Ag and u PA Ag ) , euglobulin lysis time ( ELT ) , complexes of plasmin antiplasmin ( PAP ) and fibrin degradation products ( FDP ) were tested before and after fourteen days administration of 20 30 mg / d prednisone . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In a previous work we have reported evidences on the mitogenic activity of urokinase type and tissue type plasminogen activator ( u PA , t PA ) on serum deprived human dermal fibroblasts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To determine the role of the fibrinolytic system in regulating the pathologies associated with lung injury , we examined the effect of bleomycin , an agent that induces the development of pulmonary fibrosis , in mice deficient for plasminogen ( Pg ( ) ( / ) ) , urokinase ( u PA ( ) ( / ) ) , urokinase receptor ( u PAR ( ) ( / ) ) , or tissue plasminogen activator ( t PA ( ) ( / ) ) , and in control wild type ( WT ) mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Moreover , rhIL 1alpha induced the secretion of proMMP 3 and plasminogen activator urokinase ( u PA ) from the epithelial cells , and converted the secreted proMMP 3 to the active form in the presence of plasminogen . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Fibrinopeptide A ( FP A ) , D Dimer , plasminogen activators ( PA ) of the urokinase ( u PA ) or tissue type ( t PA ) , PA inhibitor 1 ( PAI 1 ) and alpha 2 antiplasmin ( alpha 2 AP ) were determined by ELISA technique . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Increased urokinase plasminogen activator ( u PA ) production is associated with tumor invasion and metastasis in several malignancies , including breast cancer . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , the longstanding belief that t PA is responsible for physiological fibrinolysis and urokinase type PA ( u PA ) for pericellular plasminogen activation is belied by extensive experimental animal data , but these findings have had little impact on traditional thinking . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Cell surface , urokinase ( u PA ) mediated , plasminogen activation has recently been recognised as a process integral to extracellular matrix degradation . ^^^ The primary inhibitor of u PA activity in the extracellular matrix is plasminogen activator inhibitor type 2 ( PAI 2 ) , a serine protease inhibitor . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
By means of replacing part of Cytopore porous microcarriers at regular intervals , a rCHO cell line , which produces urokinase type plasminogen activitor ( u PA ) , was cultivated continuously with serum free medium in a 30 L stirred tank for 91 days . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Differences between neonates and adults in the urokinase plasminogen activator ( u PA ) pathway of the fibrinolytic system . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Blast cells from the myeloid forms of acute leukaemia ( M 2 4 ) and ' myeloid ' cell fractions from patients with chronic myeloid leukaemia were capable of lysing plasminogen containing clots ; this activity was neutralized by addition of immunoglobulin against urokinase plasminogen activator ( u PA ) , but not by anti tissue plasmogen activator ( t PA ) . ^^^ Lysed blast cells showed the presence of free plasminogen activator on sodium dodecyl sulphate polyacrylamide gel electrophoresis ( SDS PAGE ) with overlay zymography , also neutralized by anti u PA , whereas normal polymorphonuclear and mononuclear cells did not . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Importance of GlcUAbeta 1 3GalNAc ( 4S , 6S ) in chondroitin sulfate E for t PA and u PA mediated Glu plasminogen activation . ^^^ Chondroitin sulfate E ( CSE ) markedly enhanced plasminogen activation by tissue plasminogen activators ( t PAs ) and urinary plasminogen activator ( u PA ) in vitro ; in the presence of 10 microg / ml of CSE , the potentiation factors of single chain of t PA , two chain of t PA and u PA were 400 , 140 and 130 , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In these subjects , the following parameters were established : euglobulin lysis time ( ELT ) , the concentration of tissue plasminogen activator antigen ( t PA Ag ) , the concentration of urokinase plasminogen activator antigen ( u PA Ag ) , the activity of plasminogen activator inhibitor type 1 ( PAI 1 ) , the concentration of plasmin antiplasmin complex ( PAP ) and fibrinogen / fibrin degradation products ( FDP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators ( t PA and u PA ) and plasminogen activators inhibitors ( PAI 1 and PAI 2 ) in some myeloproliferative syndromes . ^^^ In the study we aimed to evaluate fibrinolysis system in blood plasma of the patients with selected myeloproliferative syndromes on the basis of examinations of plasminogen activators ( tissue t PA and urokinase type u PA ) and type 1 and type 2 plasminogen activator inhibitors ( PAI 1 and PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasmin degrades fibrin into soluble fibrin degradation products , by two physiological plasminogen activators ( PA ) , the tissue type PA ( t PA ) and the urokinase type PA ( u PA ) . t PA mediated plasminogen activation is mainly involved in the dissolution of fibrin in the circulation . u PA binds to a specific cellular receptor ( u PAR ) , resulting in enhanced activation of cell bound plasminogen . ^^^ Thus , stromelysin 1 ( MMP 3 ) cleaves a 55 kDa kringle 1 4 fragment , containing the lysine binding site ( s ) involved in cellular binding , from plasminogen and removes a 17 kDa NH 2 terminal fragment , containing the cellular receptor binding site , from urokinase ( u PA ) . ^^^ Thereby , MMP 3 may downregulate cell associated plasmin activity by decreasing the amount of activatable plasminogen , without affecting cell bound u PA activity . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
All the tumours expressed matrix metalloproteinases ( MMP ) 2 and 9 , tissue inhibitor of metalloproteases ( TIMP ) 2 , urokinase plasminogen activator ( u PA ) , plasminogen activator inhibitor ( PAI ) 1 and PAI 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The urokinase like plasminogen activator ( u PA ) present in the media conditioned by tumoral prostatic cells acting as a ligand of the cellular membrane receptor ( u PAR ) , has been identified as the specific factor that modulates this proliferative reaction . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
A growing body of evidence suggests that this process may be dependent upon plasmin , a serine protease generated from plasminogen ( Plg ) by either urokinase Plg activator ( u PA ) or tissue Plg activator ( t PA ) . ^^^ Neuritogenesis is also impaired by alpha ( 2 ) plasmin inhibitor , antibodies directed against t PA and u PA , and epsilon aminocaproic acid , a lysine analog that inhibits Plg activation and the binding of Plg to Ann 2 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Components of the urokinase plasminogen activator ( u PA ) system are involved in the metastatic process , and have accordingly been associated with clinical outcome in a variety of malignant tumours . ^^^ We investigated the prognostic importance of u PA and plasminogen activator inhibitor type 1 ( PAI 1 ) in endometrial cancer , analysed with luminometric immunoassay ( LIA ) and enzyme linked immunosorbent assay ( ELISA ) , respectively . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
DATA SOURCES : An extensive literature search ( MEDLINE , EMBASE , Conference Proceedings ) was conducted to identify articles in English published between 1966 and May 2000 pertaining to the pathophysiology of IVH and its treatment by intraventricular administration of recombinant tissue plasminogen activator ( alteplase ) or urokinase ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators ( tissue type ; t PA and urokinase type ; u PA ) and plasminogen activator inhibitors ( PAI 1 , PAI 2 ) are found in high concentrations in gingival crevicular fluid ( GCF ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators ( urokinase type , u PA and tissue type , t PA ) are serine proteases that have been suggested to play important roles in synaptic remodeling . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Urokinase plasminogen activator receptors ( u PAR ) , u PA and plasminogen activator inhibitor type 1 ( PAI 1 ) were determined by immunoassay and RNase protection assay . ^^^ TGF beta 1 dependent chemoinvasion of reconstituted basement membrane exploits the cell associated plasminogen activation system , since it is blocked by monoclonal antibodies against u PA and against various u PAR domains , as well as by anti u PAR aODN . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) is the primary inhibitor of both tissue and urokinase type plasminogen activators ( t PA , u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Treatment with MMP 3 of monocytoid THP 1 cells saturated with bound plasminogen , resulted in a dose dependent reduction of the amount of u PA activatible plasminogen . ^^^ These data thus indicate that MMP 3 may downregulate cell associated plasmin activity by decreasing the amount of activatible plasminogen , without affecting cell bound u PA activity . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activators ( t PA and u PA ) and other fibrinolysis parameters in patients with atherosclerosis obliterans and diabetic macroangiopathy ] . ^^^ Concentration of : tissue plasminogen activator antigen ( t PA : Ag ) , urokinase plasminogen activator antigen ( u PA : Ag ) , plasminogen activator inhibitor type 1 antigen ( PAI 1 : Ag ) ( ELISA ) , PAI 1 activity ( PAI 1 act . ) , euglobulin lysis time ( ELT ) ( Kowarzyk Buluk method ) , fibrinogen , fibrin degradation products ( FDP ) ( Merskey method ) in blood plasma were evaluated . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The following fibrinolysis system parameters were determined in the sera of all patients : tissue plasminogen activator concentration ( t PA : Ag ) , urokinase plasminogen activator concentration ( u PA : Ag ) , plasminogen activator inhibitor concentration ( PAI Ag ) , plasminogen level , alpha 2 antiplasmin activity ( alpha 2 AP ) , plasmin alpha 2 antiplasmin complexes concentration ( PAP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activators , urokinase PA ( u PA ) and tissue type PA ( t PA ) , are believed to play important roles in inflammatory cell infiltration , fibrin deposition , and joint destruction associated with rheumatoid arthritis ; however , their precise roles in such processes , particularly u PA , have yet to be defined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Plasminogen activator inhibitor 1 ( PAI 1 ) inhibits plasminogen activators ( u PA and t PA ) by forming stable complexes endocytosed via a low density lipoprotein receptor superfamily member dependent mechanism . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We have observed that the urokinase plasminogen activator ( u PA ) receptor is weakly upregulated by bFGF in normal satellite cells , while it is strongly up regulated by TGFbeta , mainly in dystrophic myoblasts . bFGF up regulated u PA in both normal and dystrophic myoblasts grown in primary culture , while a striking down regulation was observed with TGFbeta . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Induction of fibronectin mRNA by urokinase and tissue type plasminogen activator in human skin fibroblasts : differential role of u PA and t PA at the fibronectin protein level . ^^^ Plasminogen activators of the urokinase and tissue type and fetal calf serum ( u PA , t PA , FCS ) exert their mitogenic effect on quiescent human dermal fibroblasts and modulate the mRNA expression of cell cycle related genes . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Studies on the plasminogen activating system in gingival crevicular fluid ( GCF ) as well as gingival tissue are reviewed . t PA , u PA , PAI 1 and PAI 2 have all been detected in GCF . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
We report here that human astrocytoma cell line U 373 MG is able to express genes of the following components of plasminogen activation system : PA 1 1 , PN 1 , u PA and t PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Structural similarity of the covalent complexes formed between the serpin plasminogen activator inhibitor 1 and the arginine specific proteinases trypsin , LMW u PA , HMW u PA , and t PA : use of site specific fluorescent probes of local environment . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The plasminogen activator isolated from the venom of the snake Trimeresurus stejnegeri ( TSV PA ) triggers plasmin production , along with tissue type plasminogen activators ( t PA ) and urokinase ( u PA ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These findings illustrate the dual role of PMN in the breakdown of thrombi , in that they contribute both u PA , which lyses fibrin , and other proteases , including elastase , which can cleave fibrin and plasminogen activators / plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Enzyme linked immunosorbent assays ( ELISAs ) were used to measure cysteine cathepsin B ( CatB ) and cathepsin L ( CatL ) and their inhibitors , stefin A ( StA ) and stefin B ( StB ) , together with urokinase ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , in 150 cytosols of primary invasive breast carcinoma . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PURPOSE : The serine proteases tissue plasminogen activator ( t PA ) and urokinase plasminogen activator ( u PA ) and their inhibitor , plasminogen activator inhibitor ( PAI ) 1 , regulate a variety of processes involved in tissue morphogenesis and differentiation . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Monocytes synthesize fibrinolytic proteins , tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) , and their receptors . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
PA type was identified by fibrinography and antihuman t PA and urokinase plasminogen activator ( u PA ) blocking antibodies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Changes in urokinase plasminogen activator ( u PA ) and u PA receptor ( u PAR ) expression at the protein and mRNA level in resting neutrophils and in neutrophils activated by phorbol myristate acetate ( PMA ) were examined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Formation of covalent acyl enzyme complexes , but not noncovalent Michaelis complexes , with tissue type plasminogen activator ( t PA ) or urokinase ( u PA ) resulted in rapid decreases of fluorescence coinciding with insertion of the reactive center loop and expansion of beta sheet A . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In contrast , u PA and t PA were detectable only in the wall , suggesting that plasminogen present in the thrombus could be activated by factors secreted by the arterial wall . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both u PA inhibition and vitronectin binding by plasminogen activator inhibitor 1 regulate HT 1080 fibrosarcoma cell metastasis . ^^^ Overexpression of plasminogen activator inhibitor 1 ( PAI 1 ) reduces tumor cell migration in vitro and metastasis in mice in vivo by mechanisms involving either inhibition of urokinase plasminogen activator ( u PA ) activity or competition for an integrin binding site on vitronectin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVE : Study the expression pattern of tissue type ( t PA ) and urokinase type ( u PA ) plasminogen activators during uterine serosal healing after lesioning in a rat model . ^^^ CONCLUSION ( S ) : Altered levels of t PA and u PA transcripts and its expression in newly formed blood vessels during healing and adhesion development indicate involvement of these plasminogen activators in serosal healing and adhesion development . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Measurement of activities of urinary ( u PA ) and tissue plasminogen activator ( t PA ) and analysis of euglobulin lysis times ( ELTs ) and prekallikrein were performed simultaneously using the plasma of normal pregnant women and nonpregnant women . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
SPUC was 2 3 fold more effective in lysis of surfactant containing clots and 5 fold more resistant against plasminogen activator 1 ( PAI 1 ) as compared to the native u PA . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Plasminogen activators ( t PA and u PA ) and their inhibitors ( PAI 1 and PAI 2 ) secretions were assayed in cultures of epithelial , stromal , and trophoblast cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Furthermore , semiquantitative reverse transcription ( RT ) polymerase chain reaction ( PCR ) analysis of a panel of genes involved in the plasminogen activation system , including plasminogen activator inhibitor 1 ( PAI 1 ) , urokinase plasminogen activator ( u PA ) , and urokinase receptor ( u PAR 1 ) , demonstrated a significant upregulation ( approximately fourfold to sixfold , P < 0 . 05 ) in the expression of each of these genes in the tumor tissue . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Type 1 plasminogen activator inhibitor ( PAI 1 ) is the primary inhibitor of both tissue and urokinase type plasminogen activators ( t PA , u PA ) and is thus a primary regulator of plasminogen activation and possibly of extracellular proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Experiments were performed in a model of TNF induced hepatitis , consisting of administration of TNF in combination with D ( + ) galactosamine ( GalN ) to mice deficient in urokinase type plasminogen ( PG ) activator ( u PA ) , tissue type PG activator ( t PA ) or PG . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The following variables were determined in resting and phorbol myristate acetate activated ( 65 microM ) macrophages and heterophils : total cell associated urokinase plasminogen activator ( u PA ) , membrane bound u PA , free u PA binding sites and superoxide production . 3 . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
High molecular weight ( HMW ) urokinase plasminogen activator ( u PA ) antigen was not detected , but weak and inconsistent staining for low molecular weight ( LMW ) u PA was demonstrated on cancer cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Low molecular weight urokinase plasminogen activator ( LMW u PA ) was primarily a feature of more undifferentiated laryngeal carcinoma cells . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Several plasminogen activators ( PA ) are clinically available , including urokinase ( u PA ) , tissue plasminogen activator ( t PA ) , streptokinase ( SK ) , plasminogen streptokinase activator complex ( PSAC ) , or mutants of t PA such as reteplase ( RP ) or tenecteplase ( TP ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In this paper , we have studied the contribution of the plasminogen activation system in the development of atherosclerosis by cross breeding apoE 3 Leiden mice , which have a human like lipid profile , with mice deficient in PAI 1 ( plasminogen activator inhibitor 1 ) , u PA ( urokinase plasminogen activator ) , and t PA ( tissue plasminogen activator ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
These data indicate that the formation and stability of tubular structures by hMVEC in fibrin is accompanied by controlled fibrinolysis and depends critically not only on cell bound u PA dependent plasminogen activation , but also on the fibrin structure . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : The mRNA steady state level of a large spectrum of proteolytic enzymes ( matrix metalloproteinases : MMP 1 , 2 , 3 , 8 , 9 , 11 , 12 , 13 , 14 ; urokinase plasminogen activator : u PA ) , their physiological inhibitors ( tissue inhibitors of MMPs : TIMP 1 , 2 , 3 ; plasminogen activator inhibitor : PAI 1 ) and that of structural matrix proteins ( collagens type 1 and 3 , decorin , elastin , fibrillins 1 and 2 ) was determined by RT PCR made quantitative by using a synthetic RNA as internal standard in each reaction mixture . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The type 1 plasminogen activator inhibitor ( PAI 1 ) , the primary inhibitor of both tissue type and urokinase type plasminogen activators ( t PA , u PA ) , is the primary regulator of plasminogen activation and possibly of extracellular proteolysis . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Effects of two natural ( retinol and retinoic acid , RA ) and one synthetic N ( 4 hydroxyphenyl ) retinamide ( 4 HPR ) retinoids on proliferation and expression of urokinase plasminogen activator ( u PA ) by bovine mammary epithelial cells were examined . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both plasminogen activators ( PAs ) , tissue type ( t PA ) and urokinase ( u PA ) , were immunologically identified in all uterine biopsies . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Immunohistochemical and in situ hybridization studies of the expression of the direct activators of MMP 2 , MT 1 MMP and MT 3 MMP , and the indirect activation system tissue plasminogen activator , urokinase ( u PA ) , its receptor ( u PAR ) , and its inhibitor PAI 1 after middle cerebral artery occlusion / reperfusion were undertaken in basal ganglia samples from 26 adolescent male baboons . ^^^ The expressions of all three MMPs , u PA , u PAR , and PA 1 1 , but not tissue plasminogen activator , were increased from 1 hour after middle cerebral artery occlusion in the ischemic core . mRNA transcripts confirmed the increases in latent MMP 2 , u PA , u PAR , and PAI 1 antigen very early after middle cerebral artery occlusion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Components of the plasmin system including tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) , and plasminogen activator inhibitors PAI 1 and PAI 2 are synthesised by airway cells , and inflammatory mediators affect their expression . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Reciprocal activation of prourokinase ( pro u PA ) and plasminogen is an important mechanism in the initiation and propagation of local fibrinolytic activity . ^^^ The reciprocal activation system consists of ( 1 ) plasminogen activation by pro u PA to form plasmin , ( 2 ) pro u PA activation by the resulting plasmin to form two chain u PA ( tcu PA ) , and ( 3 ) plasminogen activation by the resulting tcu PA . ^^^ GDG significantly increased the intrinsic fluorescence of pro u PA ( 6 . 7 % ) , but not that of tcu PA or plasminogen . ^^^ The large change in intrinsic fluorescence suggests that GDG selectively affects pro u PA to alter its conformation , and this mechanism may account for enhancement of its intrinsic plasminogen activator activity . . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Quantitative gene expression analysis ( real time RT PCR ) was performed for tissue factor ( TF ) , TF pathway inhibitor ( TFPI ) , tissue type plasminogen activator ( t PA ) , urokinase type PA ( u PA ) , PA inhibitor 1 ( PAI 1 ) , and PAI 2 in peripheral white blood cells ( PBC ) as well as in alveolar macrophages ( AM ) , type 2 pneumocytes ( ATII ) , endothelial cells ( EC ) and smooth muscle cells ( SMC ) , all obtained by laser microdissection . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Blood samples were collected weekly for 8 weeks starting 4 weeks before parturition , neutrophils were isolated and the following parameters were determined in neutrophils activated by phorbol myristate acetate : total cell associated and membrane bound urokinase plasminogen activator ( u PA ) activity and superoxide production . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Factors found include tissue plasminogen activator ( t PA ) , urinary plasminogen activator ( u PA ) and plasmin . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
To evaluate the role of the 2 major matrix degrading systems , plasminogen activators ( PAs ) and matrix metalloproteinases ( MMPs ) , we compared the expression of u PA , t PA , MMP 2 and MMP 9 in ligated carotids of C 57 and FVB mice . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
METHODS : The plasma levels of tissue factor ( TF ) , thrombin antithrombin complex ( TAT ) , tissue plasminogen activator ( t PA ) , urokinase plasminogen activator ( u PA ) , urokinase plasminogen activator receptor ( u PAR ) and plasmin antiplasmin complex ( PAP ) were measured by ELISA . ^^^ CONCLUSIONS : Their existed over expression of TF and u PA , increasing formation of thrombin and plasminogen in gastric or intestinal cancer patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Inflammatory markers ( tumour necrosis factor alpha , interleukin 8 , polymorphonuclear elastase ) , fibrinolytic system variables ( tissue plasminogen activator ( PA ) , urokinase PA ( u PA ) , plasminogen activation inhibitor ( PAI ) 1 , PAI 2 ) , and several MMPs ( MMP 1 , MMP 2 , MMP 8 , MMP 9 ) and TIMPs ( TIMP 1 , TIMP 2 ) were determined by ELISA in plasma and pleural fluid . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Particularly , ras related protease expression such as the serine protease urokinase plasminogen activator ( u PA ) has been implicated in mediating cancer cell invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Brain tissue plasminogen activator ( t PA ) and urokinase ( u PA ) were quantified by using casein dependent plasminogen zymography , matrix metalloproteinase ( MMP ) 2 and MMP 9 , by MMP zymography , and tissue inhibitor of MMP ( TIMP ) 1 and 2 , by reverse zymography . ^^^ Plasminogen zymography showed a 39 % reduction of u PA in the basal ganglia ( p < 0 . 0001 ) ; t PA expression was reduced by 26 % in the cortex and by 33 % in the basal ganglia ( p < 0 . 0001 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The results showed that cyanidin 3 glucoside and cyanidin 3 rutinoside treatments could decrease the expressions of matrix matalloprotinase 2 ( MMP 2 ) and urokinase plasminogen activator ( u PA ) in a dose dependent manner and enhance the expression of tissue inhibitor of matrix matalloprotinase 2 ( TIMP 2 ) and plasminogen activator inhibitor ( PAI ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
For optimization of plasma stabilization by arginine , 50 microL pooled normal citrated plasma was incubated with 50 microL of 0 to 1500 mM arginine , pH 8 . 7 , and 25 microL 100 IU / mL u PA , 1250 IU / mL t PA , 10000 U / mL reteplase , 400 U / mL plasminogen streptokinase activator complex , 10 microg / mL tenecteplase in 6 % BSA PBS or 25 microL 25 microg / mL plasmin in 20 % glycerol . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Both urokinase plasminogen activator ( u PA ) and plasminogen activator inhibitor type 1 ( PAI 1 ) are associated with a poor prognosis in cancer patients . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Tumor associated prognostic factors of the plasminogen activator family : determination and clinical value of u PA , t PA , PAI 1 , and PAI 2 ] . ^^^ Proteolytic factors belonging t the plasminogen activator family ( plasmin , u PA , t PA , u PAR , PAI 1 , and PAI 2 ) , which usually are involved in blood clotting and degradation of blood clots , are also present in healthy and diseased tissue of the kidney , lung , liver , gastro intestinal tract , breast , prostate , ovary , and brain . ^^^ Plasminogen activators u PA and t PA , their inhibitors PAI 1 and PAI 2 , and the u PA receptor ( u PAR , CD 87 ) are often elevated in solid malignant tumour tissues compared to their normal counterparts . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Compared with those in patients without BS , plasma levels of serum creatinine ( SCr ) , triglyceride ( TG ) and plasma fibrinogen ( FIB ) were obviously higher , activated partial thromboplastin time ( APTT ) , and urokinase type plasminogen activators ( u PA ) significantly lower ; and scores of glomerular sclerosis , tubular interstitial lesions , interstitial inflammatory cell infiltration , interstitial fibrosis , tubular atrophy and vascular sclerosis significantly higher . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
An inhibitory effect of silibinin on tumor invasion and matrix metalloproteinase 2 ( MMP 2 ) and urokinasetype plasminogen activator ( u PA ) activities in culture medium has been observed in our previous study and the impacts of silibinin on enzyme activities of MMPs , u PA , mitogen activated protein kinase ( MAPK ) and Akt in A 549 cells were continued to explore in this study . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
OBJECTIVES : To evaluate urokinase plasminogen activator ( u PA ) , urokinase plasminogen activator soluble receptor ( su PAR ) , plasminogen activator inhibitor 1 ( PAI 1 ) and tissue plasminogen activator ( t PA ) plasma levels in SSc patients ( pts ) versus healthy controls and their modulation by intravenous alphacyclodestrine ( Alprostadil ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Preeclamptic women in labor showed further enhanced coagulation activation ( F ( 1+2 ) ) with raised urokinase like plasminogen activator ( u PA ) activity and reduced plasminogen activator inhibitor 2 ( PAI 2 ) levels . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Proliferation was evaluated as cell number increase , and synoviocytes were treated with 0 . 5 microM and 1 microM RAL with and without urokinase plasminogen activator ( u PA ) and anti u PA / anti u PA receptor ( u PAR ) antibodies . ^^^ Fibrinolytic system components ( u PA , u PAR and plasminogen activator inhibitor ( PAI ) 1 ) were assayed by ELISA with cells treated with 0 . 5 microM and 1 microM RAL for 48 h . u PA activity was evaluated by zymography and a direct fibrinolytic assay . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
In the absence of TGF beta 1 , the expression of thrombomodulin , the plasminogen activators u PA and t PA , and their inhibitor PAI 1 was significantly increased in the S / G2 compared to the G 1 phase . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The central components of the PA system are the proteolytic activators , urokinase plasminogen activator ( u PA ) and tissue type plasminogen activator ( t PA ) , plasminogen ( plg ) and its degradation product , plasmin , together with the major inhibitors of this system , plasminogen activator inhibitor 1 and 2 ( PAI 1 , PAI 2 ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Our results indicate that , prior to inducing a state of competency for plasminogen dependent scattering , PMA triggers an ordered succession of events where upregulation of the activity of u PA precedes proteolysis of u PAR and active degradation of the extracellular matrix ( ECM ) . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
Serum urokinase , like plasminogen activator ( u PA ) was also increased in animals with tumor , indicating tumor invasion . ^^^
Interacting proteins: P00749 and P00747	Pubmed	SVM Score :0.0
The effects of two bovine beta casein peptides on the urokinase plasminogen activator ( u PA ) system and superoxide anion ( SA ) production by porcine macrophages and neutrophils activated by phorbol myristate acetate ( PMA ) were investigated . ^^^