| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Hepatic microsomes from rats fed a crude or a purified diet were compared by measureing their contents of protein , cytochrome P 450 , and cytochrome b 5 , their rates of activity of NADPH and NADH cytochrome c reductases , NADPH cytochrome P 450 reductase , NADPH oxidase , lipid peroxidase , ethylmorphine N demethylase , aniline hydroxylase , benzpyrene hydroxylase , and their substrate binding spectra ( ethylmorphine , hexobarbital , aniline , and ethyl isoyanide ) . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Antibodies to NADH cytochrome b 5 reductase [ EC 1 . 6 . 2 . 2 ] and cytochrome b 5 inhibited NADH dependent lipid peroxidation in the presence of ADP Fe , whereas the antibody against NADPH cytochrome c reductase [ EC 1 . 6 . 2 . 4 ] showed no inhibition . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The purified proteins contain no detectable NADPH cytochrome P 450 reductase , cytochrome b 5 , or NADH cytochrome b 5 reductase , and only low levels of phospholipid ( about 1 molecule per subunit ) . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| C . 1 . 9 . 3 . 1 . ) , NADH cytochrome b 5 reductase ( NADH red . ) , NADPH cytochrome P 450 reductase ( NADPH red . ) , contents of cytochrome P 450 ( cyt . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Rabbit antisera were prepared against cytochrome b 5 and NADPH cytochrome c reductase [ EC 1 . 6 . 2 . 4 ] purified from rat liver microsomes , and utilized in examining the distribution of these and other membrane bound enzymes among the vesicles of rat liver microsomal preparations by immunoprecipitation and immunoadsorption methods . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| In rats fed commercial chow diet , cytochrome P 450 content and drug metabolizing enzyme activity in villous tip cells of duodenal mucosa were higher than in animals fed a semisynthetic diet , but cytochrome b 5 and NADPH cytochrome P 450 reductase were unaffected . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Washing the column stepwise with buffers containing suitable detergents in addition to cholate resulted in the elution of cytochrome P 450 , NADH cytochrome b 5 reductase [ EC 1 . 6 . 2 . 2 ] , NADPH cytochrome c reductase [ EC 1 . 6 . 2 . 4 ] , and cytochrome b 5 ; the former two enzymes were eluted with Emulgen 913 ( a polyoxyethylene nonylphenyl ether ) , and then the latter two were obtained separately by increasing the concentration of added deoxycholate . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| However , the extra bound detergent solubilized cytochrome b 5 did inhibit NADPH dependent N demethylations , the NADH synergism and NADPH cytochrome P 450 reductase activity . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The developmental time course fo the liver microsomal drug metabolizing activities was independent of any factor in the 105 000 g supernatant fractions and of such microsomal parameters as cytochrome b 5 and cytochrome P 450 content , and NADPH cytochrome c reductase activity , but was related to changes in NADPH cytochrome P 450 reductase levels . 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Liver weight and the ratio of liver weight to body weight were lower in the smoke exposed group , and cytochrome b 5 content and NADPH cytochrome P 450 reductase activity were higher , but cytochrome P 450 content did not differ from the control group . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Attempts to covalently link NADPH cytochrome P 450 reductase to cytochrome P 450 2B4 using a water soluble carbodiimide , 1 ethyl 3 ( 3 dimethylisopropyl ) carbodiimide , were unsuccessful , despite the fact that under the same conditions about 30 % of P 450 2B4 could be covalently linked with cytochrome b 5 in a functionally active complex ( Tamburini , P . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| In addition , a substantial similarity was shown for sequence fragments 204 224 of NADPH cytochrome P 450 reductase and 40 60 of cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Upon reconstitution with NADPH cytochrome P 450 reductase and cytochrome b 5 , the P 450 enzyme showed a high activity of 12 alpha hydroxylation with a turnover number of 36 . 6 min 1 at 37 degrees C . ^^^ The omission of either cytochrome P 450 or NADPH cytochrome P 450 reductase resulted in complete loss of activity , and the omission of cytochrome b 5 resulted in 40 % loss of activity . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Addition of purified rat liver cytochrome b 5 stimulated the 17 , 20 lyase reaction for the conversion of 17 hydroxypregnenolone to dehydroepiandrosterone , and addition of purified rat NADPH cytochrome P 450 reductase enhanced the formation of omega 1 metabolites from lauric and arachidonic acids . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Low levels of NADH cytochrome b 5 reductase ( 15 . 6 + / 6 . 3 nmol / min / mg ) and NADPH cytochrome P 450 reductase ( 14 . 5 + / 4 . 0 nmol / min / mg ) were detectable in microsomes . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The role of cytochrome b 5 in adrenal microsomal steroidogenesis was studied in guinea pig adrenal microsomes and also in the liposomal system containing purified cytochrome P 450s and NADPH cytochrome P 450 reductase . ^^^ In liposomes containing NADPH cytochrome P 450 reductase and P 450C21 or P 450 ( 17 ) alpha , lyase , addition of a small amount of cytochrome b 5 stimulated the hydroxylase activity while a large amount of cytochrome b 5 suppressed the hydroxylase activity . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Optimization of yeast expressed human liver cytochrome P 450 3A4 catalytic activities by coexpressing NADPH cytochrome P 450 reductase and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The apparent Km of p nitrophenol is also the same , as is that for NADPH cytochrome P 450 reductase and for cytochrome b 5 , which stimulates p nitrocatechol formation about 3 fold . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| However , purified P 450 IIIA1 catalyzes the oxidation of testosterone when reconstituted with NADPH cytochrome P 450 reductase , cytochrome b 5 , an extract of microsomal lipid , and detergent ( Emulgen 911 ) . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| These results , together with decreased activities of enzymes related to cytochrome P 450 mono oxygenase system , NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase , suggest that liver cells are not protected against the peroxidative stress produced by chronic administration of TAM . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| A P 450 IIIA 4 cDNA clone was expressed in yeast and the partially purified protein was used in reconstituted systems containing NADPH cytochrome P 450 reductase and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Daily administration of PB , TP , TA , or PT induced cytochrome P 450 , NADPH cytochrome P 450 reductase and / or cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The rate of testosterone oxidation was greatest when purified cytochrome P450p ( 50 pmol / ml ) was reconstituted with a fivefold molar excess of NADPH cytochrome P 450 reductase , an equimolar amount of cytochrome b 5 , 200 micrograms / ml of a chloroform / methanol extract of microsomal lipid ( which could not be substituted with dilauroylphosphatidylcholine ) , and the nonionic detergent , Emulgen 911 ( 50 micrograms / ml ) . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Distribution of cytochromes P 450 , cytochrome b 5 , and NADPH cytochrome P 450 reductase in an entire human liver . ^^^ The differences observed between locations in the microsomal concentrations of carbon monoxide binding protein ( total cytochrome P 450 ) , cytochrome b 5 , and NADPH cytochrome P 450 reductase appeared to be small and were not statistically significant . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NADPH cytochrome P 450 reductase gave a dynamic equilibrium of oxidation reduction of cytochrome b 5 in the presence of menadione ( MK ) , the level of which depended on the concentration of O 2 and superoxide dismutase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| When added to a reconstituted system containing cytochrome P 450coh , NADPH cytochrome P 450 reductase and phospholipid , cytochrome b 5 stimulates hydroxylation of coumarin and O deethylation of 7 ethoxycoumarin . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The level of quinone oxidoreductases ( microsomal and cytosolic DT diaphorase , NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase ) , superoxide dismutase and glutathione related enzymatic activities in diethylstilbestrol ( DES ) induced carcinogenesis in kidney from Syrian golden hamsters are presented . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The dependence of the mouse P 450 IA 1 specific activity on the cytochrome expression level in yeast microsomes is studied and results demonstrate that the low amounts of endogenous NADPH cytochrome P 450 reductase and cytochrome b 5 which are naturally present , are limiting for the heterologous monooxygenase activities . ^^^ The sequences encoding human liver cytochrome b 5 , the native and a modified form of the yeast NADPH cytochrome P 450 reductase were cloned by making use of PCR techniques , over expressed in yeast as functional forms , and characterized . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| In addition to NADPH cytochrome P 450 reductase , cytochrome b 5 appears to participate in the LTB 4 omega hydroxylating system , since the reaction is inhibited by an antibody raised against the cytochrome b 5 as well as one raised against the reductase . . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The content of spectrally measured cytochrome P 450 in the renal microsomes of male rats increased 2 fold with 72 h starvation , but cytochrome b 5 and NADPH cytochrome P 450 reductase were not induced . 7 Ethoxycoumarin O dealkylation and aniline hydroxylation activities of the renal microsomes of control male rats were very low but were induced 2 . 5 3 fold by 72 h starvation . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that the activation of cytochrome b 5 reduction by divalent cations in the NADH system is mainly due to an increasing accessibility of the substrate , and in the NADPH system , in addition to this , a direct effect of divalent cations on NADPH cytochrome P 450 reductase is also involved . . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The zona glomerulosa , zona fasciculata , zona reticularis , and medulla were separated from bovine adrenal glands and cytochromes P 450 and related enzymes in each zone were investigated immunochemically by Western blotting using antisera from chickens or rabbits against cytochromes P 450scc , P 450 ( 11 ) beta , P 450s21 , and b 5 , NADH cytochrome b 5 reductase , NADPH cytochrome P 450 reductase , NADPH adrenodoxin reductase , and adrenodoxin . ^^^ Concentrations of cytochrome P 450 ( 11 ) beta , NADPH cytochrome P 450 reductase , and cytochrome b 5 per milligram of protein of homogenate were higher in the zona glomerulosa than in the other zones ; the levels of the other components were higher in the zona fasciculata . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| This cytochrome P 450 efficiently catalyzed the omega hydroxylation of prostaglandin E 1 ( PGE 1 ) , prostaglandin E 2 ( PGE 2 ) , prostaglandin D 2 ( PGD 2 ) , prostaglandin F 2 alpha ( PGF 2 alpha ) , prostaglandin A 1 ( PGA 1 ) , and prostaglandin A 2 ( PGA 2 ) , as well as the omega and ( omega 1 ) hydroxylation of myristate and palmitate , in a reconstituted system containing cytochrome P 450 , NADPH cytochrome P 450 reductase , phospholipid , and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The interaction of heme nonapeptide ( a proteolytic product of cytochrome c ) with purified NADH : cytochrome b 5 ( EC 1 . 6 . 2 . 2 ) and NADPH : cytochrome P 450 ( EC 1 . 6 . 2 . 4 ) reductases was investigated . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Evidence of binary complex formations between cytochrome P 450 , cytochrome b 5 , and NADPH cytochrome P 450 reductase of hepatic microsomes . ^^^ Water soluble carbodiimide catalyzed cross linking of purified cytochrome P 450 LM 2 , cytochrome b 5 , and NADPH cytochrome P 450 reductase was used to identify stable complexes formed between these proteins . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| We applied this irradiation technique to the rat liver microsomal membrane proteins : cytochrome b 5 , epoxide hydrolase , flavin containing monooxygenase , NADH ferricyanide reductase , NADPH cytochrome P 450 reductase , and seven different forms of cytochrome P 450 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The cholesterol 7 alpha hydroxylase system of untreated rabbit liver microsomes was reconstituted with the purified P 450 , NADPH cytochrome P 450 reductase , and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Treatment of phenobarbital at the oral dose of 120 mg / kg induced significant increases in the contents of cytochrome P 450 , cytochrome b 5 and NADPH cytochrome P 450 reductase and in the activity of ethoxycoumarin O deethylase , and significant decreases in the activities of benzphetamine N demethylase and aniline hydroxylase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Cytochrome P 450 , NADPH cytochrome P 450 reductase and cytochrome b 5 levels were also unchanged , while slight increases were detected in NADH cytochrome b 5 reductase and epoxide hydrase activities . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Cytochrome P 450A had a requirement for conspecific ( scup ) NADPH cytochrome P 450 reductase and all reconstituted activities examined were stimulated by the addition of purified scup cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The partially purified cytochrome P 450 efficiently catalyzed the omega hydroxylation of various prostaglandins such as PGE 1 , PGE 2 , PGF 2 alpha , PGD 2 , and PGA 1 in a reconstituted system containing NADPH cytochrome P 450 reductase , cytochrome b 5 , and phosphatidylcholine . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| In contrast , amidination , or acetylation of amino groups , or amidination of amino groups plus methylamidination of the carboxyl groups did not appreciably slow the rate of reduction of the cytochrome b 5 by NADPH cytochrome P 450 reductase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NADPH cytochrome P 450 reductase cytochrome b 5 interactions : crosslinking of the phospholipid vesicle associated proteins by a water soluble carbodiimide . ^^^ Detergent solubilized and purified rabbit liver microsomal NADPH cytochrome P 450 reductase and cytochrome b 5 were coreconstituted into phospholipid vesicles . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| This study compared the NADH and NADPH supported p nitrophenetole ( NP ) O deethylase , ethylmorphine ( EM ) O deethylase and EM N demethylase activities of rat hepatic microsomes with respect to dioxygen requirement , inhibition by carbon monoxide , inhibition by classical inhibitors of cytochrome P 450 systems , and the involvement of NADH cytochrome b 5 , cytochrome b 5 reductase and NADPH cytochrome P 450 reductase . ^^^ The use of antibodies against NADPH cytochrome P 450 reductase , NADH cytochrome b 5 reductase and cytochrome b 5 demonstrated that both the NADH and the NADPH supported reactions depend on established components of cytochrome P 450 systems . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Cytochrome P 450ca efficiently catalyzed the omega hydroxylation of prostaglandin A 1 ( PGA 1 ) and the omega and ( omega 1 ) hydroxylation of caprate , laurate , and myristate in the reconstituted system containing cytochrome P 450ca , NADPH cytochrome P 450 reductase , cytochrome b 5 , and phosphatidylcholine . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The effects of cytochrome b 5 with manganese protoporphyrin 9 substituted for heme were compared with those of native cytochrome b 5 and the apoenzyme on the oxygenation of substrates in the reconstituted system containing liver microsomal cytochrome P 450 , NADPH cytochrome P 450 reductase , and phosphatidylcholine . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The content of cytochrome b 5 and the activities of NADPH cytochrome P 450 reductase and of NADH cytochrome b 5 reductase , however , corresponded to those of rodent lung microsomes , with mean values of 0 . 12 nmol / mg protein , 0 . 039 and 3 . 58 mumol / min / mg protein , respectively . . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The rate of LTB metabolism was also measured in reconstituted systems of both micelles and phospholipid vesicles containing cytochrome P 450 LM 2 , NADPH cytochrome P 450 reductase , and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| A mechanism for electron transfer from NADH to cytochrome b 5 is discussed on the basis of the one electron redox potentials of the enzyme and is compared with the electron transfer mechanism of NADPH cytochrome P 450 reductase . . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Possible involvement of NADPH cytochrome P 450 reductase and cytochrome b 5 on beta ketostearoyl CoA reduction in microsomal fatty acid chain elongation supported by NADPH . ^^^ When anti NADPH cytochrome P 450 reductase or anti cytochrome b 5 immunoglobulin G were included in this system , not only was the overall chain elongation significantly suppressed , but also a new radioactive product , which was identified as 2 heptadecanone derived from an intermediate beta ketostearate , is accumulated depending upon the amounts of IgG added . ^^^ These results suggest that NADPH cytochrome P 450 reductase and cytochrome b 5 participate in the conversion from beta ketostearoyl CoA to beta hydroxystearoyl CoA , which is the first reductive step of the microsomal chain elongating system . . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The enhancement by cytochrome b 5 was more pronounced when lower concentrations of either the substrate or NADPH cytochrome P 450 reductase were added to the reconstituted system . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Recent reports have shown the participation of cytochrome b 5 and NADPH cytochrome P 450 reductase ( FPT ) on NADPH initiated microsomal fatty acid chain elongation . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Immunoreactive cytochrome b 5 and NADPH cytochrome P 450 reductase ( EC 1 . 6 . 2 . 4 ) from rat liver microsomal fractions were measured by using an enzyme linked immunoadsorbent assay ( e . l . i . s . a . ) as a function of age , sex and type of inducer ( phenobarbital or 3 methylcholanthrene ) , and the values were compared with those obtained by spectral measurement ( for cytochrome b 5 ) or enzymic assay ( for reductase ) . ^^^ An enzyme linked immunoadsorbent assay for measuring cytochrome b 5 and NADPH cytochrome P 450 reductase in rat liver microsomal fractions . ^^^ In untreated animals , there was more cytochrome b 5 and NADPH cytochrome P 450 reductase when measured by an e . l . i . s . a . than was seen spectrally or enzymically . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Four protein components of the hepatic microsomal electron transfer system , NADPH cytochrome P 450 reductase , cytochrome P 450 , NADH cytochrome b 5 reductase , and cytochrome b 5 , all purified from liver microsomes of phenobarbital pretreated rabbits , were co reconstituted into liposomes of egg yolk phosphatidylcholine . ^^^ The results obtained led to the following conclusions : ( 1 ) The first of the two electrons required for the monooxygenase reaction is exclusively supplied via NADPH cytochrome P 450 reductase , whereas the second one is preferentially supplied via cytochrome b 5 . ( 2 ) The rate limiting step of the overall monooxygenase reaction is the introduction of the second electron , or a step later than that , if the second electron is sufficiently supplied . ( 3 ) All four proteins seem to distribute randomly on the plane of liposomal membranes , and the interaction between them is caused by the lateral diffusion of the proteins . . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Both the cytochrome b 5 level and NADH cytochrome b 5 reductase activity in rat liver microsomes were increased 2 fold by repeated i . p . administration of 1 . 5 mmol / kg propylthiouracil ( PTU ) for 2 weeks , but neither the cytochrome P 450 level nor NADPH cytochrome P 450 reductase activity were affected by the treatment . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| These reactions were absolutely dependent on cytochrome P 450p 2 and NADPH cytochrome P 450 reductase , and stimulated by addition of phosphatidylcholine and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The cytochrome efficiently catalyzed the hydroxylation of fatty acids as well as prostaglandins in a reconstituted system containing cytochrome P 450 , NADPH cytochrome P 450 reductase , phospholipid , and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Neither agent decreases the microsomal concentration of cytochrome b 5 , NADH cytochrome b 5 reductase , or NADPH cytochrome P 450 reductase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Activation or inhibition by cytochrome b 5 of benzphetamine N demethylation was studied in micelle reconstituted systems containing cytochrome P 450 LM 2 , NADPH cytochrome P 450 reductase , and dilauroyl phosphatidylcholine . ^^^ The function of cytochrome b 5 was interpreted in terms of a model in which inhibition of cytochrome P 450 mediated reactions results from changes in phospholipid protein interactions and activation occurs via facilitation of electron transfer between NADPH cytochrome P 450 reductase and cytochrome P 450 in the membrane . . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| A protein protein association of cytochrome P 450 LM 2 with NADPH cytochrome P 450 reductase , with cytochrome b 5 , and with both proteins was demonstrated in reconstituted phospholipid vesicles by magnetic circular dichroism difference spectra . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The syntheses of cytochrome b 5 and NADPH cytochrome P 450 reductase ( fpT ) were also examined . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| This cytochrome hydroxylated PGA 1 at the omega position with a turnover rate of 38 . 2 nmol / min / nmol of cytochrome P 450 in the reconstituted system containing cytochrome P 450 , NADPH cytochrome P 450 reductase , cytochrome b 5 and phosphatidylcholine . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The structural identification of the reactive free radical metabolite and the product of its addition to phospholipids was accomplished by use of a reconstituted system of human cytochromes P 450 , NADPH cytochrome P 450 reductase , and cytochrome b 5 in phospholipid vesicles . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| On the other hand , the rate of P 450 ( 1 ) reduction was not affected by the addition of cytochrome b 5 to the system and that of cytochrome b 5 reduction by NADPH cytochrome P 450 reductase was sufficient to support electron flow to cytochrome P 450 via cytochrome b 5 as the second electron . ^^^ The stimulatory effect of cytochrome b 5 on the P 450 ( 1 ) catalyzed monooxygenase reactions can be explained by assuming that the rate of the second electron supply to the oxygenated P 450 ( 1 ) substrate complex from cytochrome b 5 is higher than that directly from NADPH cytochrome P 450 reductase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| A similar linear relationship between the reducibility of cytochrome b 5 and the negative charge of the liposomes was also evident in membranes containing NADPH cytochrome P 450 reductase and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| In hepatic microsomes , cytochrome b 5 reductase ( EC 1 . 6 . 2 . 2 ) activity was unaffected by riboflavin deficiency , although NADPH cytochrome c reductase ( EC 1 . 6 . 2 . 4 ) and microsomal flavin content were diminished to approximately half the control values . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Both P 450 isoenzymes , termed PB 1 and PB 4 , when reconstituted with purified rat liver NADPH cytochrome P 450 reductase and cytochrome b 5 , generated 4 tolyl ethyl sulfoxide which was predominantly in the S ( ) configuration . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| In this paper we showed age effect on NADPH cytochrome P 450 reductase activity , cytochrome b 5 and NADH cytochrome b 5 reductase from rat liver . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Dynamic interactions of rabbit liver cytochromes P450IA2 and P450IIB4 with cytochrome b 5 and NADPH cytochrome P 450 reductase in proteoliposomes . ^^^ Purified liver microsomal cytochrome P450IA2 or P450IIB4 was co reconstituted with cytochrome b 5 or NADPH cytochrome P 450 reductase in phosphatidylcholine phosphatidylethanolamine phosphatidylserine vesicles at a lipid to P 450 weight ratio of 2 by cholate dialysis procedures . ^^^ The two cytochrome P450s have shown very different interactions with cytochrome b 5 and NADPH cytochrome P 450 reductase . ^^^ By the presence of the redox partner , the mobile population of cytochrome P450IA2 was increased significantly from 80 % to 96 % ( plus cytochrome b 5 ) and to 89 % ( plus NADPH cytochrome P 450 reductase ) due to dissociation of P 450 oligomers . ^^^ On the other hand , the mobility of cytochrome P450IIB4 was not considerably affected by the presence of cytochrome b 5 or NADPH cytochrome P 450 reductase as judged by little difference in phi and r 3 , keeping the mobile population of 100 % . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| A catalytic activity of the CYP3A7 expressed in the Sf 9 cells was confirmed by the umu gene expression mutation assay , in which aflatoxin B 1 was activated to a mutagen by the expressed CYP3A7 in the presence of NADPH cytochrome P 450 reductase and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Purified CYP2B4 , reconstituted in the presence of NADPH cytochrome P 450 reductase and cytochrome b 5 , metabolized arachidonic acid , producing primarily EETs . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Parameters investigated were : microsomal protein , NADPH cytochrome P 450 reductase activity , NADH cytochrome b 5 reductase activity , cytochrome b 5 content , cytochrome P 450 content , aminopyrine N demethylase activity , aniline p hydroxylase activity , p nitroanisole O demethylase activity , uridine diphosphate ( UDP ) glucuronyl transferase activity and glutathione S transferase activity . ^^^ By 48 h post mortem , NADPH cytochrome P 450 reductase activity decreased to 91 % , NADH cytochrome b 5 reductase activity decreased to 94 % , and cytochrome P 450 content decreased to 92 % of relative activities . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Hydroxylation rates were determined under a variety of conditions , including two concentrations of NADPH cytochrome P 450 reductase ( 2 and 16 nmol / nmol P 450 ) and the absence or presence of cytochrome b 5 ( 2 nmol / nmol P 450 ) . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| These enzymes included DT diaphorase , NADH cytochrome b 5 reductase , and NADPH cytochrome P 450 reductase . ^^^ All the tumors subjected to enzyme profiling contained the bioreductive enzymes , DT diaphorase , NADH cytochrome b 5 reductase and NADPH cytochrome P 450 reductase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The same treatment did not alter the liver microsomal cytochrome b 5 content , NADH cytochrome b 5 reductase and NADPH cytochrome P 450 reductase activities . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The specific content of cytochrome b 5 was 16 . 5 nmol / mg protein and purified cytochrome b 5 fractions were free of cytochrome P 450 , NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase activities . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Xenobiotic metabolism in humanized yeast : engineered yeast cells producing human NADPH cytochrome P 450 reductase , cytochrome b 5 , epoxide hydrolase and P 450s . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : The electron transfer system molecules , NADPH cytochrome P 450 reductase ( Red ) and cytochrome b 5 ( b 5 ) are known to increase the relative activity of 17 , 20 lyase to 17 alpha hydroxylase in vitro . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Purified CYP3A5 in a reconstituted system , consisting of dilauroylphosphatidylcholine , cytochrome b 5 , and NADPH cytochrome P 450 reductase , and an NADPH regenerating system displayed a 2 fold greater rate of 1 ' hydroxymidazolam formation and a similar rate of 4 hydroxymidazolam formation compared with a reconstituted system with CYP3A4 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The production of adrenal androgens can be modulated by the activities of steroidogenic enzymes and by the electron transfer system , NADPH cytochrome P 450 reductase ( Red ) and cytochrome b 5 ( b 5 ) , both of which have been shown to increase 17 , 20 lyase activity in vitro . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Because of its strategic location in the protein , the Tyr 74 residue was mutated to a lysine in order to investigate how it affected the interaction of heme with the protein and whether it might be an alternative binding site and an electron transfer path which cytochrome b 5 would use with its amphipathic electron transfer partners cytochrome P 450 and its corresponding NADPH cytochrome P 450 reductase . ^^^ The mutant protein receives electrons from NADPH cytochrome P 450 reductase and provides the second electron to cytochrome P 450 to catalyze the metabolism of methoxyflurane , a substrate which requires cytochrome b 5 for its metabolism , at the same rate as the wild type protein . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| We investigated whether the electron transfer systems NADPH cytochrome P 450 reductase and cytochrome b 5 , both of which had been shown to increase 17 , 20 lyase activity in vitro , were involved in the reaction selectivity between 17 alpha hydroxylase and 17 , 20 lyase in adrenocortical adenomas obtained from eight patients with Cushing ' s syndrome producing different concentrations of adrenal androgen . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| After preservation or reperfusion , the liver microsomes were prepared and the concentration of each component of the P 450 system [ NADPH cytochrome b 5 reductase ( b 5 reductase ) , cytochrome b 5 ( b 5 ) , NADPH cytochrome P 450 reductase ( P 450 reductase ) and cytochrome P 450 ( P 450 ) ] and their drug metabolizing activities and concentration of apo cytochrome P 450 2E1 ( apo P 450 2E1 ) were measured . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The cytochrome P 450 and cytochrome b 5 contents , the NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase activities , aniline hydroxylase and 4 aminopyrine N demethylase activities were measured in the hepatic microsomal fraction . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Histidine residues in rabbit liver microsomal cytochrome P 450 2B4 control electron transfer from NADPH cytochrome P 450 reductase and cytochrome b 5 . ^^^ However , there was a steady attenuation of NAD ( P ) H driven electron flux with increasing extent of P 450 2B4 carbethoxylation in reconstituted systems fortified with either NADPH cytochrome P 450 reductase or NADH cytochrome b 5 reductase / cytochrome b 5 as the redox partners , with 50 % inhibition occurring when 6 7 histidines were blocked . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Glycerol had no effect on the content of cytochrome b 5 or activities of NADPH cytochrome P 450 reductase or NADH cytochrome b 5 reductase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| This treatment also results in increases in NADPH cytochrome P 450 reductase and P glycoprotein ( the MDR 1 gene product ) but has no detectable effect on expression of CYP1A1 , CYP2D6 , cytochrome b 5 , liver or intestinal fatty acid binding proteins , or villin . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Recombinant CYP2C8 , CYP2C19 , two allelic variants of CYP2C18 , and six variants of CYP2C9 expressed in a yeast cDNA expression system were each enzymatically active , as judged by the ability of the isolated microsomes to catalyse 7 ethoxycoumarin O deethylation after reconstitution with purified NADPH cytochrome P 450 reductase and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The mechanism of cytochrome P 450 2B4 self inactivation during catalytic turnover has been studied in monooxygenase reconstituted system containing from monomers of the membrane proteins : NADPH cytochrome P 450 reductase , cytochrome P 450 2B4 and cytochrome b 5 in presence of detergent emulgen 913 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The present study reports on the effects of horminone on serum alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) levels , on hepatic cytochrome P 450 ( P 450 ) and cytochrome b 5 ( cyt b 5 ) contents and on the activities of NADPH cytochrome P 450 reductase ( NR ) , mixed function mono oxygenases ( MFO ) , glutathione S transferase ( GST ) and glutathione reductase ( GR ) of Wistar male rat . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Recombinant P 450 3A7 was partially purified using ion exchange and hydroxylapatite chromatography and reconstituted with NADPH cytochrome P 450 reductase , cytochrome b 5 , and lipids . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Reconstitution premixes for assays using purified recombinant human cytochrome P 450 , NADPH cytochrome P 450 reductase , and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Metabolism in lysates of cells infected with recombinant baculovirus was greatly enhanced by the addition of purified housefly NADPH cytochrome P 450 reductase and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| A preliminary study of microsomal enzyme activity analysis revealed a suppression effect of liver cytochrome P 450 dependent monooxygenase activities , including cytochrome P 450 , cytochrome b 5 , and benzo ( a ) pyrene hydroxylase , but an increased level of kidney cytochrome P 450 dependent monooxygenase activities , including NADPH cytochrome P 450 reductase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| BG was shown to inactivate P 450 3A4 in a reconstituted system consisting of purified P 450 3A4 , NADPH cytochrome P 450 reductase , cytochrome b 5 , and phospholipids . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The role of the NH 2 terminal hydrophobic patch of cytochrome P4502B4 ( CYP2B4 ) in interactions with NADPH cytochrome P 450 reductase ( P450R ) and cytochrome b 5 ( b 5 ) was assessed using a variant lacking the signal anchor sequence ( Delta 2 27 ) . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Reaction rates were maximal when CYP 4A2 was reconstituted in the presence of an equimolar concentration of cytochrome b 5 and a 10 fold molar excess of NADPH cytochrome P 450 reductase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Cytochrome P 450 and cytochrome b 5 contents , NADPH cytochrome P 450 reductase , NADH cytochrome b 5 reductase , aniline hydroxylase and 4 aminopyrine N demethylase activities were measured in a hepatic microsomal fraction . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Betaine treated either 4 or 24 hr prior to sacrifice did not alter the hepatic contents of cytochrome P 450 , cytochrome b 5 , or NADPH cytochrome P 450 reductase activity . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| In a reconstituted system with insect NADPH cytochrome P 450 reductase , cytochrome b 5 , and NADPH , the purified CYP4C7 metabolized ( 2E , 6E ) farnesol to a more polar product that was identified by GC MS and by NMR as ( 10E ) 12 hydroxyfarnesol . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The following parameters were determined : cytochrome P 450 content , cytochrome b 5 content , NADPH cytochrome P 450 reductase activity , and NADH cytochrome b 5 reductase activity , succinate dehydrogenase ( SDH ) activity , and lactate dehydrogenase ( LDH ) activity . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Major increases were observed in several microsomal proteins , including NADPH cytochrome P 450 reductase , cytochrome b 5 and serine protease inhibitor . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The recombinant 6beta B protein showed similar turnover rate and regioselectivity for testosterone with purified P4506beta 4 by the simultaneous addition of NADPH cytochrome P 450 reductase and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The primate adrenal zona reticularis is defined by expression of cytochrome b 5 , 17alpha hydroxylase / 17 , 20 lyase cytochrome P 450 ( P450c17 ) and NADPH cytochrome P 450 reductase ( reductase ) but not 3beta hydroxysteroid dehydrogenase / delta5 4 isomerase ( 3beta HSD ) . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The widely accepted catalytic cycle of cytochromes P 450 ( CYP ) involves the electron transfer from NADPH cytochrome P 450 reductase ( CPR ) , with a potential for second electron donation from the microsomal cytochrome b5 / NADH cytochrome b 5 reductase system . ^^^ This alternative catalytic cycle , where both the first and second electrons were donated via the NADH cytochrome b 5 electron transport system , can account for the continued ergosterol production seen in yeast strains containing a disruption of the gene encoding CPR . . ^^^ Using purified proteins including Candida albicans CYP 51 and yeast NADPH cytochrome P 450 reductase , cytochrome b 5 and NADH cytochrome b 5 reductase , we show here that fungal CYP 51 mediated sterol 14alpha demethylation can be wholly and efficiently supported by the cytochrome b5 / NADH cytochrome b 5 reductase electron transport system . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| EE / LE inhibited not only the metabolic capacity of P 450 , a protein which directly undergoes suicide inhibition , but also the level of rat liver cytochrome b 5 ( dependent on the heme pool ) as well as the activities of NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase in the liver and kidney . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Cotransfection of vectors expressing cytochrome b 5 and NADPH cytochrome P 450 reductase indicates that both are required for an optimum production of DHEA . . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The obtained results have shown an overall decrease of liver microsomal cytochrome P 450 , cytochrome b 5 , NADPH cytochrome P 450 reductase , NADH cytochrome b 5 reductase , and aniline hydroxylase with a subsequent decrease of phase 2 enzymes , glutathione S transferase and UDP glucuronyl transferase in cancer bearing animals . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Significant amounts of P 450 and cytochrome b 5 and activities of NADPH cytochrome P 450 reductase were detected in microsomes from rabbit duodenal , jejunal , ileac and colon mucosa . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Extract treatment significantly increased the basal levels of acid soluble sulphydryl ( SH ) content , cytochrome P 450 , NADPH cytochrome P 450 reductase , cytochrome b 5 , NADH cytochrome b 5 reductase , glutathione S transferase , DT diaphorase , superoxide dismutase , catalase , glutathione peroxidase and glutathione reductase in the liver . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The hepatic content of cytochrome P 450 , cytochrome b 5 and NADPH cytochrome P 450 reductase were determined at 4 , 8 , 24 , 72 h , one and two weeks after the second dose of treatment . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Significant increase in the activities of acid soluble sulfhydryl ( SH ) content , cytochrome P 450 , NADPH cytochrome P 450 reductase , cytochrome b 5 , NADH cytochrome b 5 reductase , glutathione S transferase ( GST ) , DT diaphorase ( DTD ) , superoxide dismutase ( SOD ) , catalase ( CAT ) , glutathione peroxidase ( GPx ) and glutathione reductase ( GR ) were observed in the liver at both dose levels of treatments . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Significant increases in the levels of acid soluble sulfhydryl ( SH ) content , NADPH cytochrome P 450 reductase , NADH cytochrome b 5 reductase , glutathione S transferase ( GST ) , DT diaphorase ( DTD ) , superoxide dismutase ( SOD ) , catalase , glutathione peroxidase ( GPX ) and glutathione reductase ( GR ) were observed in the liver . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Estimations of the rate of CPR ( 0 . 3 s ( 1 ) ) or cytochrome b 5 ( 9 . 1 s ( 1 ) ) binding with CYP4A7 were also determined . . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Drug metabolizing enzymes are called mixed function oxidase or monooxygenase and containing many enzymes including cytochrome P 450 , cytochrome b 5 , and NADPH cytochrome P 450 reductase and other components . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Selection for azole resistance following expression of a cDNA library constructed with RNA from progesterone treated R . nigricans in the yeast Saccharomyces cerevisiae led to the identification of CPR 1 FL and CYB 5 1 cDNAs , which code for functionally competent NADPH cytochrome P 450 reductase and cytochrome b 5 , respectively . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| A significant increase was observed in the levels of NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductases after antitubercular drug administration . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Homotropic and heterotropic phenomena are encountered in both substrate activation and productive coupling of the electron donors NADPH cytochrome P 450 reductase and cytochrome b 5 , and the lipid environment of the system also appears to play a role as an effector . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| We transfected human embryonic kidney ( HEK 293 ) cells with various amounts of vectors expressing P450c17 , NADPH cytochrome P 450 reductase , and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| In a low but physiological concentration of NADPH cytochrome P 450 reductase and excess amount of progesterone , outer mitochondrial membrane cytochrome b 5 stimulated the cytochrome P 45017alpha catalyzed reactions , 17alpha hydroxylation and C 17 C20 bond cleavage . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Ascorbic acid and alpha tocopherol treatment decreases the activity of haem oxygenase , whereas it increases the levels / activity of cytochrome P 450 , cytochrome b 5 and NADPH cytochrome P 450 reductase in arsenic intoxicated rats . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| METHODS : Real time reverse transcriptase polymerase chain reaction was used to quantify transcripts encoding steroidogenic enzymes and the cofactors steroidogenic acute regulatory protein ( StAR ) , cytochrome b 5 ( CYb 5 ) , and P 450 oxidoreductase ( POR ) . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Biphasic effects of the flavonoids quercetin and naringenin on the metabolic activation of 2 amino 3 , 5 dimethylimidazo [ 4 , 5 f ] quinoline by Salmonella typhimurium TA 1538 co expressing human cytochrome P 450 1A2 , NADPH cytochrome P 450 reductase , and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Topological changes in the CYP3A4 active site probed with phenyldiazene : effect of interaction with NADPH cytochrome P 450 reductase and cytochrome b 5 and of site directed mutagenesis . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| On the other hand , molecular diversity has also been reported for NADPH cytochrome P 450 reductase , cytochrome b 5 , and cytochrome b 5 reductase in plants . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| The AFM images of the full length P 450 2B4 system ' s constituent components cytochrome P 450 2B4 ( 2B4 ) , NADPH cytochrome P 450 reductase and cytochrome b 5 ( b 5 ) , were obtained on highly oriented pyrolitic graphite . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Two cistronic expression plasmids for the wild type solubilized domain of porcine NADPH cytochrome P 450 reductase ( PsCPR ) gene in Escherichia coli were systematically constructed using a solubilized domain of porcine cytochrome b 5 gene ( Psb 5 gene ) or a derivative of it as the first cistron to examine their utility for second gene expression preventing the translational inhibition caused by the intramolecular local secondary structure of mRNA at the ribosome binding site ( RBS ) . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Various spectrophotometric assays have been performed to examine electron accepting properties of CPR and its ability to reduce cytochrome b 5 , cytochrome c , and ferricyanide . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Reduction of cytochrome b 5 by NADPH cytochrome P 450 reductase . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| This ratio is regulated posttranslationally by at least three factors : the abundance of the electron donating protein P 450 oxidoreductase ( POR ) , the presence of cytochrome b 5 and the serine phosphorylation of P450c17 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| However , the requirement for NADPH cytochrome P 450 reductase , often cytochrome b 5 , and an expensive cofactor , NADPH , complicates the design of mammalian P 450 enzymes as biocatalysts . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| Testosterone , androstenedione and progesterone were oxidized with housefly cytochrome P 450 6A1 enzyme reconstituted in vitro with housefly NADPH cytochrome P 450 reductase and cytochrome b 5 . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| L carnitine , combined with the lower dose of methanol , stimulated NADPH cytochrome P 450 reductase after 48 h and cytochrome b 5 and NADH cytochrome b 5 reductase over the whole period of observation . ^^^ |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P00167 and P16435 |
Pubmed |
SVM Score :0.0 |
| NA |
|