Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.79941254
When a small amount of NADH cytochrome b 5 reductase is bound liposomes simultaneously with cytochrome b 5 , the two proteins catalyze the reduction of cytochrome c by NADH . 0.79941254^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.61485954
Interactions between NADH cytochrome b 5 reductase and cytochrome b 5 preparations purified from liver microsomes . 0.61485954^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.88559599
To identify the cytochrome b 5 residues responsible for the electrostatic interaction with NADH cytochrome b 5 reductase ( b5R ) , we prepared and characterized the cytochrome b 5 mutants in which Glu 41 , Glu 42 , Glu 63 , Asp 70 , and Glu 73 were replaced by Ala , utilizing site directed mutagenesis and the expression system for cytochrome b 5 in Escherichia coli . 0.88559599^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Immunological similarity between NADH cytochrome b 5 reductase of erythrocytes and liver microsomes . ^^^ In a number of animal species soluble NADH cytochrome b 5 reductase of erythrocytes was compared with membrane bound NADH cytochrome b 5 reductase of liver microsomes by using an antibody to purified NADH cytochrome b 5 reductase from rat liver microsomes . ^^^ The data with erythrocyte ghosts suggested that cytochrome b 5 and NADH cytochrome b 5 reductase are also present in the ghost . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The results indicate that the oxygen consumption with alpha NADH is due to electron transfer from alpha NADH via NADH cytochrome b 5 reductase and cytochrome b 5 , in which the rate determining step lies at some reaction after the reduction of cytochrome b5 . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The three purified proteins which are required for microsomal stearyl CoA desaturation , NADH cytochrome b 5 reductase , cytochrome b 5 , and desaturase , have been combined with egg lecithin or dimyristyl lecithin vesicles to reconstruct a functional electron transport system capable of utilizing NADH and O 2 in the desaturation of stearyl CoA . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The NADH dependent stearoyl CoA desaturase of hepatic microsomes ( EC 1 . 14 . 99 . 5 ) is an enzyme system consisting of cytochrome b 5 reductase ( EC 1 . 6 . 2 . 2 ) , cytochrome b 5 , and the terminal desaturase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The presence of NADH cytochrome b 5 reductase [ EC 1 . 6 . 2 . 2 ] in microsomes from anaerobically grown yeast was confirmed by its isolation and purification . ^^^ Purification and characterization of NADH cytochrome b 5 reductase . ^^^ Although some differences exist between the properties of NADH cytochrome b 5 reductase from yeast and from mammalia , the results indicate a functional similarity of the present enzyme to mammalian NADH cytochrome b 5 reductase in the microsomal electron transport system . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The heme fragment can mediate electron transfer between pig liver microsomal NADH cytochrome b 5 reductase and cytochrome c . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Both cytochrome b 5 and NADH cytochrome b 5 reductase ( EC 1 . 6 . 2 . 2 ) are required for these studies and a method is described for the purification of homogeneous preparations of detergent isolated cytochrome b 5 from rat liver . 6 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Hepatic NADH cytochrome b 5 reductase was reduced by 1 mol of dithionite or NADH per mol of enzyme bound FAD , without forming a stable semiquinone or intermediate during the titrations . ^^^ Potentiometric titration of NADH cytochrome b 5 reductase at pH 7 . 0 with dithionite gave a midpoint potential of 258 mV ; titration with NADH gave 160 mV . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Measurement of the microsomal electron transfer components showed no significant changes in the NADH cytochrome b 5 reductase activity or in the concentration of cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Among the respiratory pigments analysed spectrophotometrically in the microsomal fraction prepared from ascites cells , only small amounts of flavoproteins ( NADH cytochrome b 5 reductase and NADPH cytochrome c reductase ) were detectable . 3 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase [ EC 1 . 6 . 2 . 2 ] has been solubilized with Triton 10 100 and purified to homogeneity from rabbit liver microsomes . ^^^ Purification and properties of the intact form of NADH cytochrome b 5 reductase from rabbit liver microsomes . ^^^ NADH cytochrome b 5 reductase [ EC 1 . 6 . 2 . 2 ] has been solubilized with Triton 10 100 and purified to homogeneity from rabbit liver microsomes . ^^^ It is concluded that the intact form of NADH cytochrome b 5 reductase is an amphipathic protein and its hydrophobic moiety , which is removable by lysosomal digestion , is responsible for the tight binding of the reductase to microsomes and for its normal functioning in the membrane . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The purified proteins contain no detectable NADPH cytochrome P 450 reductase , cytochrome b 5 , or NADH cytochrome b 5 reductase , and only low levels of phospholipid ( about 1 molecule per subunit ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Carboquone was found to greatly stimulate the aerobic NADH oxidation in the presence of both the partially purified NADH cytochrome b 5 reductase and cytochrome b 5 prepared from hepatic microsomes by acting as an electron carrier from cytochrome b 5 to molecular oxygen . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Antibodies to NADH cytochrome b 5 reductase [ EC 1 . 6 . 2 . 2 ] and cytochrome b 5 inhibited NADH dependent lipid peroxidation in the presence of ADP Fe , whereas the antibody against NADPH cytochrome c reductase [ EC 1 . 6 . 2 . 4 ] showed no inhibition . ^^^ These oberservations suggest that the electron from NADH was supplied to the lipid peroxidation reaction via NADH cytochrome b 5 reductase and cytochrome b 5 . ^^^ In this case , the antibody against cytochrome b 5 could not inhibit the reaction , while the antibody against NADH cytochrome b 5 reductase did inhibit it , suggesting the direct transfer of electrons from NADH cytochrome b 5 reductase to EDTA Fe complex . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
A soluble form of NADH cytochrome b 5 reductase ( NADH : ferricytochrome b 5 oxidoreductase , EC 1 . 6 . 2 . 2 ) was found in the cytosolic fraction of rabbit liver . ^^^ Soluble NADH cytochrome b 5 reductase from rabbit liver cytosol : partial purification and characterization . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Evidence for the involvement of a microsomal electron transport system in the enzymic conversion of alkylacyl glycero 3 phosphorylethanolamine to ethanolamine plasmalogen was obtained by using specific antibodies against NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
C . 1 . 9 . 3 . 1 . ) , NADH cytochrome b 5 reductase ( NADH red . ) , NADPH cytochrome P 450 reductase ( NADPH red . ) , contents of cytochrome P 450 ( cyt . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
When the antibody against NADPH cytochrome c reductase was used , however , immunoadsorption of microsomes on Sepharose bound antibody produced some separation of NADPH cytochrome c reductase and cytochrome P 450 from NADH cytochrome b 5 reductase and cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The activities and contents of microsomal mono electron carriers ( NADPH cytochrome c reductase , NADH cytochrome b 5 reductase , cytochrome P 450 and cytochrome b 5 ) in the liver of 160 pregnant rabbits and their response to phenobarbital ( PB ) administration were found to be different to that of non pregnant animals and to vary depending on the period of gestation . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Kinetic studies on methemoglobin reduction by human red cell NADH cytochrome b 5 reductase . ^^^ The intermediate hemoglobins which were produced by the partial reduction of methemoglobin with human red cell NADH cytochrome b 5 reductase were fractionated by the preparative isoelectric focusing . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The extent of solubilization was compared with that of protein , rotenone insensitive NADH cytochrome c reductase or NADH cytochrome b 5 reductase , which have been shown to associate with microsomal membrane rather than luminar contents . ^^^ When 0 . 05 % of DOC which was capable of releasing luminar contents of microsomal vesicles was applied to microsomal suspension , only a limited part of NADH cytochrome b 5 reductase , rotenone insensitive NADH cytochrome c reductase or T 4 5 ' deiodinase activity was solubilized . ^^^ Solubilization of protein , NADH cytochrome b 5 reductase or rotenone insensitive NADH cytochrome c reductase was increased abruptly to 66 % , 58 % or 63 % , respectively . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Microsomal NADH cytochrome b 5 reductase is an amphiphilic protein consisting of a hydrophilic ( catalytic ) region and a hydrophobic ( membrane binding ) segment . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Both activities were reconstituted in the presence of deoxycholate on addition of the other components of the cytochrome b 5 electron transport chain ( cytochrome b 5 and NADH cytochrome b 5 reductase ) to the solubilized desaturase ; addition of lecithin further stimulated the activities . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Microsomes , peroxisomes , and outer mitochondrial membranes , all containing endogenous cytochrome b 5 , incorporated large amounts of the hemoprotein in such a way that it was reducible by an inherent NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The activities and the content of enzymes involved in mixed function oxidase systems ( cytochromes P 450 and b 5 , NADPH cytochrome c reductase and NADH cytochrome b 5 reductase ) in microsomes from 114 human placentae were found to increase from the 34th up to the 38th 39th week of gestation and later to decrease until the 43rd week . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Dimyristoyl lecithin liposomes , containing cytochrome b 5 reductase ( NADH : ferricytochrome b 5 oxidoreductase , EC 1 . 6 . 2 . 2 ) and varying amounts of cytochrome b 5 , were used to measure flavoprotein catalysis alone and catalysis requiring electron transfer between the reductase and cytochrome as a function of temperature . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The present experiment suggests that the protein factor is not NADH cytochrome b 5 reductase , cytochrome b 5 , or the cyanide sensitive factor . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The microsomal NADH cytochrome b 5 reductase activity was definitely low in the patient ' s thyroid . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Electron transport from untreated to mersalyzed microsomal vesicles at the level of NADH cytochrome b 5 reductase or cytochrome b 5 has been demonstrated in the absence of added water soluble electron carriers . ^^^ The obtained data suggest that the capability to carry out intermembrane electron transfer is specific for NADH cytochrome b 5 reductase and / or cytochrome b 5 , localized in microsomal and outer mitochondrial membranes . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Immunochemical similarity of soluble methemoglobin reductase and cytochrome b 5 of human erythrocytes with NADH cytochrome b 5 reductase and cytochrome b 5 of rat liver microsomes . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
On the contrary , cytochrome b 5 and NADH cytochrome b 5 reductase activity was inhibited after phenobarbital injection . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Washing the column stepwise with buffers containing suitable detergents in addition to cholate resulted in the elution of cytochrome P 450 , NADH cytochrome b 5 reductase [ EC 1 . 6 . 2 . 2 ] , NADPH cytochrome c reductase [ EC 1 . 6 . 2 . 4 ] , and cytochrome b 5 ; the former two enzymes were eluted with Emulgen 913 ( a polyoxyethylene nonylphenyl ether ) , and then the latter two were obtained separately by increasing the concentration of added deoxycholate . ^^^ NADH cytochrome C reductase activity could be observed at high efficiency under appropriate conditions in a system containing the cytochrome b 5 and NADH cytochrome b 5 reductase preparations . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase readily binds to preformed phospholipid vesicles either below or above the phase transition temperature of the lipid and in the absence of detergents . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Similarity to the liver microsomal NADH cytochrome b 5 reductase and cytochrome b 5 enzyme system . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Addition of AA to the PTU containing diet further increased the content of cytochromes P 450 and b 5 , but not the activities of NADPH cytochrome c and NADH cytochrome b 5 reductase , and the drug metabolizing enzymes . 3 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Twenty four hours after poly 1 : poly C administration , hepatic P 450 content decreased to 57 % of control , whereas depression of other microsomal enzymes was less pronounced : P 450 reductase ( 69 % ) , cytochrome b 5 ( 74 % ) and NADH cytochrome b 5 reductase ( 85 % ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Low levels of NADH cytochrome b 5 reductase ( 15 . 6 + / 6 . 3 nmol / min / mg ) and NADPH cytochrome P 450 reductase ( 14 . 5 + / 4 . 0 nmol / min / mg ) were detectable in microsomes . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Two forms of soluble NADH cytochrome b 5 reductase were purified from human erythrocytes . ^^^ Two distinct fractions both having the NADH cytochrome b 5 reductase activity eluted from the second DEAE cellulose column were further purified by ultrafiltration and 5 ' ADP agarose affinity chromatography . 2 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
These results , together with decreased activities of enzymes related to cytochrome P 450 mono oxygenase system , NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase , suggest that liver cells are not protected against the peroxidative stress produced by chronic administration of TAM . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The treatment of ZD rats with Cd reduced the activity of liver microsomal stearoyl CoA desaturase by 90 % without any changes in the activity of NADH cytochrome b 5 reductase or in the microsomal level of cytochrome b 5 whereas the activity of stearoyl CoA desaturase was reduced by only 35 % by the treatment of ZA rats with Cd . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Calf thymus DNA was incubated with bleomycin and FeCl 3 in the presence of isolated rat liver microsomal NADH cytochrome b 5 reductase , cytochrome b 5 and NADH which catalyze redox cycling of the bleomycin Fe complex . ^^^ These results indicate that NADH cytochrome b 5 reductase catalyzes redox cycling of the bleomycin Fe complex which results in the formation of reactive oxygen species which oxidize deoxyribose as well as bases of DNA . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Characterization of lysyl residues of NADH cytochrome b 5 reductase implicated in charge pairing with active site carboxyl residues of cytochrome b 5 by site directed mutagenesis of an expression vector for the flavoprotein . ^^^ An expression vector for bovine NADH cytochrome b 5 reductase was constructed from two DNA fragments that were derived from beef liver poly ( A+ ) RNA using the polymerase chain reaction . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The content of cytochrome P 450 , cytochrome b 5 and activity of NADH cytochrome b 5 reductase did not change . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
As . reacted with both fully reduced and semiquinone forms of hepatic NADH cytochrome b 5 reductase with second order rate constants of 4 . 3 10 10 ( 6 ) and 3 . 7 10 10 ( 5 ) M 1 s 1 , respectively , at pH 7 . 0 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Structural role of serine 127 in the NADH binding site of human NADH cytochrome b 5 reductase . ^^^ Serine 127 of human NADH cytochrome b 5 reductase was replaced by proline and alanine by site directed mutagenesis . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The pooled samples of respiratory nasal epithelium contained , relative to liver , a low amount of cytochrome P 450 ( about 25 pmol / mg protein ) and associated biotransformation activities , and a low level of other components of the mixed function oxidase system such as cytochrome b 5 , NADH and NADPH cytochrome c reductase however the NADH cytochrome b 5 reductase activity was comparable to that of liver . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Furthermore , when the fraction containing cytochrome b 5 and NADH cytochrome b 5 reductase was added to the fraction containing beta ketoreductase activity , no increase in beta ketoreductase activity was observed . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Hereditary methemoglobinemia is an autosomal recessive disorder characterized by NADH cytochrome b 5 reductase ( b5R ) deficiency . ^^^ Serine proline replacement at residue 127 of NADH cytochrome b 5 reductase causes hereditary methemoglobinemia , generalized type . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The level of quinone oxidoreductases ( microsomal and cytosolic DT diaphorase , NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase ) , superoxide dismutase and glutathione related enzymatic activities in diethylstilbestrol ( DES ) induced carcinogenesis in kidney from Syrian golden hamsters are presented . ^^^ A dramatic decrease in microsomal and cytosolic DT diaphorase activities ( 13 . 6 and 37 . 8 % of controls ) , as well as in glutathione disulphide reductase ( 39 . 5 % ) , and less marked in superoxide dismutase ( 45 . 6 % ) , NADH cytochrome b 5 reductase ( 61 . 9 % ) glutathione transferase ( GST ) towards 1 chloro 2 , 4 dinitrobenzene ( CDNB ) ( 66 . 2 % ) and glutathione peroxidase ( GSH Px ) ( 80 % ) activities , were observed in kidneys with pre and neoplastic lesions . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Microsomal cytochrome P 450 concentration and NADH cytochrome b 5 reductase activity were increased both in liver ( to 1 2 months ) and in kidney ( to 2 4 months ) , and then were decreased . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Also , amounts of cytochrome b 5 in testicular microsomal fraction were decreased markedly after irradiation , but no significant change of NADH cytochrome b 5 reductase activity was observed in the treated pups . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Purification of NADH cytochrome b 5 reductase from sheep lung and its electrophoretic , spectral and some other properties . 1 . ^^^ NADH cytochrome b 5 reductase was purified from sheep lung microsomes in the presence of non ionic and ionic detergents , Emulgen 913 and cholate , respectively . 2 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase is known to be located on two distinct membranes , i . e . endoplasmic reticulum and outer mitochondrial membranes . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
A significantly changing influence of benzene on the activity of NADH cytochrome b 5 reductase was observed both in smooth and rough fractions . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The zona glomerulosa , zona fasciculata , zona reticularis , and medulla were separated from bovine adrenal glands and cytochromes P 450 and related enzymes in each zone were investigated immunochemically by Western blotting using antisera from chickens or rabbits against cytochromes P 450scc , P 450 ( 11 ) beta , P 450s21 , and b 5 , NADH cytochrome b 5 reductase , NADPH cytochrome P 450 reductase , NADPH adrenodoxin reductase , and adrenodoxin . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Isolated and purified microsomal NADH cytochrome b 5 reductase ( EC 1 . 6 . 2 . 2 ) was incubated with bleomycin ( BLM ) and FeCl 3 in the presence of NADH . ^^^ Redox cycling of bleomycin Fe ( 3 ) and DNA degradation by isolated NADH cytochrome b 5 reductase : involvement of cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
No significant change was observed in NADH cytochrome b 5 reductase and NADPH cytochrome c reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Purification and properties of a membrane bound NADH cytochrome b 5 reductase from erythrocytes of the sipunculid worm , Phascolopsis gouldii . ^^^ The purification to homogeneity of the membrane bound NADH cytochrome b 5 reductase from erythrocytes of the sipunculid , Phascolopsis gouldii is reported . ^^^ The P . gouldii NADH cytochrome b 5 reductase is the first from hemerythrin containing erythrocytes to be purified and characterized . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Autosomal recessive hereditary methemoglobinemia associated with mental retardation is a rare syndrome caused by a deficiency of the enzyme NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
In a female newborn presenting with pronounced cyanosis in the absence of cardiopulmonary disease , the cyanosis was due to methemoglobinemia of 33 % at birth and 17 % at 24 hours ( upper limit 0 . 5 % ) which was found to be secondary to deficiency of red blood cell cytochrome b 5 reductase ( EC 1 . 6 . 2 . 2 . ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Correlation of enzyme activities with fluorescence anisotropy of dansyl labeled cytochrome b5 / NADH cytochrome b 5 reductase systems in phosphatidylcholine vesicles . ^^^ The changes in steady state fluorescence lifetimes and anisotropy decay parameters , as well as enzyme activities , of dansyl labeled cytochrome b 5 ( DNS cytochrome b 5 ) , on interaction with NADH cytochrome b 5 reductase in DMPC vesicles , have been measured as a function of temperature . ^^^ These results show that interaction of vesicle bound DNS cytochrome b 5 and NADH cytochrome b 5 reductase leads to an increased degree of order of the dansyl labeled cytochrome with little change in its rotational flexibility , and suggests that the increased order can be correlated with increased enzyme activity . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Biochemical studies had already suggested that mutation at the cytochrome b 5 reductase locus ( DIA 1 ) is responsible for RCM . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Purification and characterization of the human neutrophil NADH cytochrome b 5 reductase . ^^^ NADH cytochrome b 5 reductase is the predominant NADH diaphorase found in the human neutrophil ( Blood 62 : 152 , 1983 ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Palmitoyl CoA elongation in brain microsomes : dependence on cytochrome b 5 and NADH cytochrome b 5 reductase . ^^^ Mercuric chloride and p chloromercuriphenylsulfonate , inhibitors on NADH cytochrome b 5 reductase , at 32 microM inhibited NADH supported palmitoyl CoA elongation to 30 and 60 % of control activity , respectively , whereas NADPH supported palmitoyl CoA elongation was unaffected by these mercurials . ^^^ An antibody to rat liver NADH cytochrome b 5 reductase inhibited brain microsomal NADH cytochrome b 5 reductase activity and NADH dependent palmitoyl CoA elongation . ^^^ Treatment of brain microsomes with trypsin diminished the cytochrome b 5 content ; NADH and NADPH cytochrome c reductase activities were significantly decreased , but the decrease in NADH cytochrome b 5 reductase activity was relatively small . ^^^ These results indicate the presence of an electron transport system , NADH NADH cytochrome b 5 reductase cytochrome b 5 fatty acid elongation , in brain microsomes . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The interaction of heme nonapeptide ( a proteolytic product of cytochrome c ) with purified NADH : cytochrome b 5 ( EC 1 . 6 . 2 . 2 ) and NADPH : cytochrome P 450 ( EC 1 . 6 . 2 . 4 ) reductases was investigated . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
A cDNA coding for human liver NADH cytochrome b 5 reductase ( cytochrome b 5 reductase , EC 1 . 6 . 2 . 2 ) was cloned from a human liver cDNA library constructed in phage lambda gt 11 . ^^^ Molecular cloning of cDNAs of human liver and placenta NADH cytochrome b 5 reductase . ^^^ The library was screened by using an affinity purified rabbit antibody against NADH cytochrome b 5 reductase of human erythrocytes . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Cytochrome b 5 and NADH cytochrome b 5 reductase from sipunculan erythrocytes ; a methemerythrin reduction system from Phascolopsis gouldii . ^^^ We also report the isolation and purification of a membrane bound NADH cytochrome b 5 reductase from these erythrocytes . ^^^ These observations suggest that cytochrome b 5 and NADH cytochrome b 5 reductase in sipunculan erythrocytes function to counteract autoxidation of oxyHr . ^^^ The sequence of electron flow in the system of purified components is : NADH NADH cytochrome b 5 reductase cytochrome b 5 metHr . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Posttranslational iron insertion into the apoproteins , either in vitro with membrane preparations or by iron addition during induction , results in the formation of active holoenzyme which can be reconstituted with NADH cytochrome b 5 reductase and cytochrome b 5 to form an active stearyl CoA desaturase system . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Cloning and chromosomal mapping of human cytochrome b 5 reductase ( DIA 1 ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Cytochrome P 450 , NADPH cytochrome P 450 reductase and cytochrome b 5 levels were also unchanged , while slight increases were detected in NADH cytochrome b 5 reductase and epoxide hydrase activities . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
By comparing the Arabidopsis protein sequence with other protein sequences , three functional domains were deduced : ( 1 ) a molybdenum pterin binding domain that is similar to the molybdenum pterin binding domain of rat liver sulfite oxidase , ( 2 ) a heme binding domain that is similar to proteins in the cytochrome b 5 superfamily , and ( 3 ) an FAD binding domain that is similar to NADH cytochrome b 5 reductase . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
But INH had little effect on the content of cytochrome b 5 and NADH cytochrome b 5 reductase activity . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Furthermore , the Km for the flavoprotein in supporting either free LM 2 or LM 2 cyt b 5 dependent p nitroanisole demethylation were similar . ( 4 ) Less than 20 25 % of the cyt b 5 within the complex could be reduced by free NADH cytochrome b 5 reductase ( NADH cyt b 5 reductase ) albeit at a slow rate . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
This phenomenon was ascribed to a decrease in the activity of NADH cytochrome b 5 reductase in the mitochondrial outer membrane due to CCl 4 treatment . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Cytochrome P 450 and monooxygenase activity shows decrease , but cytochrome b 5 and NADH cytochrome b 5 reductase activity increases . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase and cytochrome b 5 were not affected by the phosphatase either . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The toxin treated animals exhibited reduced levels of liver cytochrome P 450 and cytochrome b 5 , accompanied by significant lowering of NADPH cytochrome c reductase and NADH cytochrome b 5 reductase activities . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The purified preparations of hepatic P 450MCI and P 450MCII contained 9 . 6 and 8 . 3 nmol cytochrome P 450 ( P 450 ) per mg protein , respectively , and were essentially free from NADPH cytochrome c ( P 450 ) reductase ( fpT ) , NADH cytochrome b 5 reductase and cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Increases in cytochrome b 5 content and NADH cytochrome b 5 reductase activity were slower than increases in cytochrome P 450 content and NADPH cytochrome c reductase activity . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Recessively inherited NADH cytochrome B 5 reductase deficiency , when present in the homozygous or doubly heterozygous form , is manifested by two different clinical presentations , depending on the nature and cellular distribution of the mutant enzyme . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Identification of the essential cysteine residue of NADH cytochrome b 5 reductase . ^^^ The soluble catalytic domain of NADH cytochrome b 5 reductase was radiolabeled with [ 14C ] N ethylmaleimide . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Indomethacin in vitro also caused a loss of cytochrome b 5 , NADH cytochrome b 5 reductase , NADPH cytochrome c reductase and epoxide hydrolase activities , but an activation of UDP glucuronyltransferase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Propylthiouracil , a selective inhibitor of NADH cytochrome b 5 reductase . ^^^ Propylthiouracil inhibited the activity of NADH cytochrome b 5 reductase of rat liver microsomes using potassium ferricyanide as electron acceptor . ^^^ NADH supported reduction of cytochrome b 5 was also inhibited by propylthiouracil in the reconstituted system consisting of cytochrome b 5 and partially purified NADH cytochrome b 5 reductase . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Complete amino acid sequence of steer liver microsomal NADH cytochrome b 5 reductase . ^^^ The complete covalent structure of liver microsomal NADH cytochrome b 5 reductase from steer liver microsomes was determined . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The use of antibodies against NADPH cytochrome P 450 reductase , NADH cytochrome b 5 reductase and cytochrome b 5 demonstrated that both the NADH and the NADPH supported reactions depend on established components of cytochrome P 450 systems . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Substantial one step enrichments of NADH cytochrome b 5 reductase and an unstable cytochrome b 5 dependent oxidase of cholesterol synthesis , 4 methyl sterol oxidase , have been obtained on d b 5 columns which further supports this conclusion . ^^^ NADH cytochrome b 5 reductase and the high affinity isozyme of cytochrome P 450 purified by d b 5 affinity chromatography are poorly retained on t b 5 columns . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Gastric microsomal NADH cytochrome b 5 reductase : characterization and solubilization . ^^^ NADH cytochrome b 5 reductase from hog gastric microsomes was studied with respect to substrate dependence , optimum pH , thermal denaturation as well as anti cytochrome b 5 antibodies and different ions . ^^^ We therefore suggest that gastric microsomes contain a Triton soluble membrane bound NADH cytochrome b 5 reductase which is in many respects similar to the liver and red cell enzymes . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Optimizations of conditions for reconstitution have been obtained ; both cytochrome b 5 and NADH cytochrome b 5 reductase serve as electron carriers . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The final enzyme preparation was free from other electron transfer components and phospholipids in microsomes , and the desaturation reaction was reconstituted with the following components : NADH , molecular oxygen , phospholipids and three proteins , i . e . , NADH cytochrome b 5 reductase , cytochrome b 5 and the terminal oxidase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Fly ash , its benzene extract and benzene extracted residue significantly increased the levels of cytochrome P 450 , cytochrome b 5 and the activities of NADPH cytochrome c reductase , NADH cytochrome b 5 reductase , aminopyrine N demethylase and glutathione S transferase in a dose dependent manner . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The inhibitory effect of several halides and carboxylates on bovine liver microsomal NADH : cytochrome b 5 oxidoreductase ( EC 1 . 6 . 2 . 2 ) was examined . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Cucumber monodehydroascorbate reductase occurs in soluble form and can be distinguished from NADPH dehydrogenase , NADH dehydrogenase , DT diaphorase , microsome bound NADH cytochrome b 5 reductase , and NADPH cytochrome c reductase by its molecular weight , amino acid composition , and specificity of electron acceptors and donors . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The involvement of NADH cytochrome b 5 reductase and cytochrome b 5 complex in microsomal NADH cytochrome c reductase activity . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The involvement of NADH cytochrome b 5 reductase and cytochrome b 5 complex in microsomal NADH cytochrome c reductase activity . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
UDP glucuronyl transferase activity was 50 % higher in Mp , but glucose 6 phosphatase and NADH cytochrome b 5 reductase activities were 25 % higher in Ms . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The increase in the activity of microsomal stearoyl CoA desaturation by clofibric acid feeding was due to the increase in the activity of terminal desaturase as measured by the rate constant for cytochrome b 5 reoxidation , but not due to the changes in cytochrome b 5 content and NADH cytochrome b 5 reductase activity . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The increase in the activity of stearoyl CoA desaturation by peroxisome proliferators appears to be due to an increase in activity only of the terminal desaturase , but not to changes in either NADH cytochrome b 5 reductase activity or cytochrome b 5 content . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Protection from the inhibitory action of sulfhydryl reagents on NADH cytochrome b 5 reductase was also observed in the presence of n octylamine . 4 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
It is antigenically distinct from the ubiquitous red cell type NADH diaphorase ( soluble cytochrome b 5 reductase ) specified by the DIA 1 locus . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH oxidation is blocked by mersalyl , an inhibitor of NADH cytochrome b 5 reductase . ^^^ It is concluded that aerobic oxidation of exogenous NADH involves the following pathway : NADH leads to NADH cytochrome b 5 reductase leads to cytochrome b 5 leads to intermembrane cytochrome c leads to cytochrome oxidase leads to oxygen . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The enzyme from the control LCL ( c 252 / B 95 ) was found to be immunologically related to the human soluble erythrocyte cytochrome b 5 reductase , indicating that it is the product of the same gene : the DIA 1 ( diaphorase ) locus . ^^^ Recessive congenital methemoglobinemia ( RCM ) is due to the homozygous deficiency of NADH cytochrome b 5 reductase ( EC 1 . 6 . 2 . 2 . ) . ^^^ NADH cytochrome b 5 reductase activity in lymphoid cell lines . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH oxidation , both in intact and in water treated mitochondria , is 90 % inhibited by mersalyl , an inhibitor of the outer membrane NADH cytochrome b 5 reductase , and 10 % inhibited by rotenone . ^^^ It is concluded that ( 1 ) oxidation of exogenous NADH in the presence of cytochrome c proceeds mostly through NADH cytochrome b 5 reductase and cytochrome b 5 on the outer membrane and then through cytochrome oxidase via the cytochrome c shuttle , and ( 2 ) ATP synthesis during oxidation of exogenous NADH is partly due to oxidation of endogenous substrates and partly to operation of cytochrome oxidase receiving electrons from the outer membrane via cytochrome c . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The content of cytochrome b 5 and the activities of NADPH cytochrome P 450 reductase and of NADH cytochrome b 5 reductase , however , corresponded to those of rodent lung microsomes , with mean values of 0 . 12 nmol / mg protein , 0 . 039 and 3 . 58 mumol / min / mg protein , respectively . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
One electron oxidation reduction properties of hepatic NADH cytochrome b 5 reductase . ^^^ The one electron oxidation reduction properties of flavin in hepatic NADH cytochrome b 5 reductase were investigated by optical absorption spectroscopy , electron paramagnetic resonance ( EPR ) , and potentiometric titration . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
In the presence of low concentrations of Triton 10 100 , it interacts effectively with the intact form of NADH cytochrome b 5 reductase purified either from yeast microsomes or from rabbit liver microsomes . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
It is reducible by NADH in the presence of an NADH cytochrome b 5 reductase purified from rat liver microsomes . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
It was shown that the NADH cytochrome c reductase activity characterizing the NADH cytochrome b 5 reductase and cytochrome b 5 interaction sharply decreased in the immobilized system as compared to that in solution . ^^^ This , the reconstitution in the immobilized phospholipid layer allowed to characterize NADH cytochrome b 5 reductase as a system operating on occasional collisions of its components . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Extra hepatic Walker sarcoma 256 produced a marked decrease ( approximately 60 % ) in the levels of cytochrome P 450 and NADP cytochrome P 450 reductase in rat liver endoplasmic reticulum , and a lesser decrease ( approximately 20 % ) of cytochrome b 5 and NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Four protein components of the hepatic microsomal electron transfer system , NADPH cytochrome P 450 reductase , cytochrome P 450 , NADH cytochrome b 5 reductase , and cytochrome b 5 , all purified from liver microsomes of phenobarbital pretreated rabbits , were co reconstituted into liposomes of egg yolk phosphatidylcholine . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Identification of the NH 2 terminal blocking group of NADH cytochrome b 5 reductase as myristic acid and the complete amino acid sequence of the membrane binding domain . ^^^ The NH 2 terminal blocking group of the membrane binding domain of NADH cytochrome b 5 reductase has been deduced as myristic ( n tetradecanoyl ) acid . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Selective induction of cytochrome b 5 and NADH cytochrome b 5 reductase by propylthiouracil . ^^^ Both the cytochrome b 5 level and NADH cytochrome b 5 reductase activity in rat liver microsomes were increased 2 fold by repeated i . p . administration of 1 . 5 mmol / kg propylthiouracil ( PTU ) for 2 weeks , but neither the cytochrome P 450 level nor NADPH cytochrome P 450 reductase activity were affected by the treatment . ^^^ From the above evidence , repeated administration of PTU selectively induced cytochrome b 5 and NADH cytochrome b 5 reductase in rat liver microsomes . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
These compounds had no influence on NADH cytochrome b 5 reductase activity , cytochrome b 5 content and terminal desaturase activity . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
It was demonstrated that NADH , molecular oxygen , phospholipid , and three enzymes : NADH cytochrome b 5 reductase , cytochrome b 5 , and the terminal oxygenase , were absolutely essential for lathosterol 5 desaturation in the reconstituted system . ^^^ Furthermore , the rate of the NADH depending lathosterol 5 desaturation in the reconstitution system , was proportional to the concentration either of the terminal desaturase , cytochrome b 5 , or NADH cytochrome b 5 reductase , under conditions in which other enzymes were present in excess . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The patient and several members of her family showed decreased activities of erythrocyte NADH cytochrome b 5 reductase , predisposing them to the development of clinically significant methaemoglobinaemia when challenged with oxidant drugs . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Isolation and partial characterization of the NH 2 terminal membrane binding domain of NADH cytochrome b 5 reductase . ^^^ The amphipathic flavoprotein NADH cytochrome b 5 reductase from steer liver was converted into the membrane binding and soluble catalytic domains by controlled subtilisin proteolysis of vesicle bound , externally oriented reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The ATP dependent proteolytic system present in reticulocytes can release the active hydrophilic domain of cytochrome b 5 and NADH cytochrome b 5 reductase from the endoplasmic reticulum , that in mature erythrocytes act as methemoglobin reductase . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Covalent cross linking of the active sites of vesicle bound cytochrome b 5 and NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
We measured cytochrome b 5 reductase ( EC 1 . 6 . 2 . 2 ) activity in erythrocytes and leukocytes from subjects of both sexes ranging in age from neonate to adulthood , all either apparently healthy or without hematologic disorders . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Neither agent decreases the microsomal concentration of cytochrome b 5 , NADH cytochrome b 5 reductase , or NADPH cytochrome P 450 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The major mechanism for this reductive capacity resides in the soluble cytochrome b5 / NADH cytochrome b 5 reductase of the cytosol which presumably arise from the microsomal proteins of the endoplasmic reticulum through proteolytic cleavage of the proteins ' hydrophobic tails during the maturation of nucleated erythrocyte precursors . ^^^ NADH cytochrome b 5 reductase is coded for by a gene on the human chromosome 22 . ^^^ Type 2 , severe lethal methemoglobinemia is a generalized disorder in which the NADH cytochrome b 5 reductase is apparently defective in all tissues . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The purified preparations containing 1 . 74 nmol per mg protein were essentially free of NADH cytochrome b 5 reductase and NADPH cytochrome c reductase , and included a small amount of cytochrome b 5 spectrophotometrically . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The inhibitor of mitochondrial NADH cytochrome b 5 reductase dicumarol prevented the cytochrome P 450 reduction in the presence of glutamate , NAD+ and amytal but did not affect the reduction of cytochrome P 450 by the added NADH . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Vesicles containing bound cytochrome b 5 reductase and cytochrome b 5 in order to complete an NADH cytochrome b 5 reductase system also utilized reduced cytochrome b 5 as the electron donor for this peroxidative step . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Clofibrate also promoted a decrease in serum cholesterol and triacylglycerol levels , delta 6 desaturase activity and a suppression of the electron transport chain as measured by NADH cytochrome b 5 reductase and NADH cytochrome c reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Also , we find that a fragment of rat liver NADH cytochrome b 5 reductase can restore NADH dependent cytochrome b 5 reduction to Tetrahymena microsomes which have been treated with N ethylmaleimide to eliminate endogenous reductase activity . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
These results suggest that the NADH cytochrome b 5 reductase of human red cell membranes is identical with that of liver microsomes and that the enzyme of red cell cytosol is a proteolytic product of the membrane enzyme . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Stearyl CoA was shown to stimulate the reoxidation rate of cytochrome b 5 of gastric microsomes and to decrease the reduction rate of trypsin purified hog liver cytochrome b 5 by the NADH cytochrome b 5 reductase of these microsomes . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Properties of NADH cytochrome b 5 reductase from human neutrophils . ^^^ The specificities of electron donors ( e . g . , NADH congruent to deamino NADH much greater than NADPH ) and acceptors ( e . g . , Fe ( CN ) 6 3 greater than 2 , 6 dichlorophenol indophenol much greater than O 2 ) for the enzyme ( s ) in unfractionated membranes , along with action of inhibitors ( e . g . , ADP , p chloromercuribenzoate ) and the pH optimum , indicate that virtually all of the membrane bound ferricyanide reductase activity in these cells is NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The concentration of cytochrome b 5 and the activity of NADH cytochrome b 5 reductase decreased exponentially with the age of red cells . ^^^ The decline of cytochrome b 5 seemed to be more rapid than the decline in the activities of glutamate oxaloacetate transaminase and NADH cytochrome b 5 reductase whose apparent half lives were 210 and 240 days , respectively . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
One percent of the Algerian population carries a weak allele at the DIA 1 locus , responsible for a 50 % decrease of red cell soluble cytochrome b 5 reductase activity . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The purified preparation contained 12 . 5 nmol of cytochrome P 450 per mg protein and was essentially free from NADPH cytochrome c reductase , NADH cytochrome b 5 reductase , and cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
An electron flow from NADH via cytochrome b 5 can be utilized as the second electron for the O deethylase reaction of 7 ethoxycoumarin catalyzed by reconstituted systems containing P 450 ( 2 ) and P 448 ( 2 ) when both NADH cytochrome b 5 reductase and cytochrome b 5 are included in the system , although the cytochrome has no stimulatory effect at all on the deethylase activity of these two cytochrome P 450 ' s . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Special emphasis is directed to the major and minor metabolic pathways in human erythrocytes that are involved in the reduction of methemoglobin , and evidence is presented that the NADH methemoglobin reductase system , involving a soluble cytochrome b 5 and NADH cytochrome b 5 reductase , it is the most important reductive mechanism . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
In hepatic microsomes , cytochrome b 5 reductase ( EC 1 . 6 . 2 . 2 ) activity was unaffected by riboflavin deficiency , although NADPH cytochrome c reductase ( EC 1 . 6 . 2 . 4 ) and microsomal flavin content were diminished to approximately half the control values . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Purification and properties of soluble NADH cytochrome b 5 reductase of rabbit erythrocytes . ^^^ Soluble NADH cytochrome b 5 reductase was purified from rabbit erythrocytes to homogeneity by simple procedures developed in this study including fractionation with ammonium sulfate , gel filtration on a Sephadex G 75 column , and affinity chromatography on a 5 ' AMP Sepharose 4B column . ^^^ The enzyme was purified about 12 , 000 fold from hemolysate in terms of NADH cytochrome b 5 reductase activity with a high yield of 40 % . ^^^ The NADH cytochrome b 5 reductase activity decreased strikingly as the buffer or salt concentration in the assay mixture was increased , and the optimal pH for the reduction of cytochrome b 5 with NADH was determined to be 6 . 6 in Tris maleate buffer of constant ionic strength . ^^^ The maximum velocity of NADH cytochrome b 5 reductase activity of the purified enzyme was very high , 1 , 280 mumol / min / mg of protein in 10 mM phosphate buffer ( pH 6 . 6 ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Prenatal diagnosis of congential enzymopenic methaemoglobinaemia ( CEM ) with mental retardation was performed in two fetuses at risk for generalized NADH cytochrome b 5 reductase deficiency . ^^^ The pregnancy was terminated , and the diagnosis of homozygous NADH cytochrome b 5 reductase deficiency was confirmed in cord blood cells , in several different tissues and in cultured fibroblasts from the aborted fetus . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Since the activity of NADH cytochrome b 5 reductase was extremely low in the surviving erkythrocytes , the difference in proportion of ferric hemoglobins ( valency hjybrids + methemoglobin ) in these two fractions might be due simply to the difference in pH between intracellular and extracellular fluids , which is largely reflected in the oxidation rate of hemoglobin . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Of the enzyme activities integrated in the microsomal electron transport chains , NADH cytochrome b 5 reductase was not affected by fibrate treatment , NADPH cytochrome c reductase activity was enhanced ( BFB = GFB > CFB ) , whereas NADH cytochrome c reductase activity was reduced by CFB and BFB . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
In this paper we showed age effect on NADPH cytochrome P 450 reductase activity , cytochrome b 5 and NADH cytochrome b 5 reductase from rat liver . ^^^ NADH cytochrome b 5 reductase activity increased to 1 month of life , but in older group decreased . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Transient kinetics of reduction and interprotein electron transfer in the human cytochrome b 5 reductase cytochrome b 5 ( b5R b 5 ) system was studied by laser flash photolysis in the presence of 5 deazariboflavin and EDTA at pH 7 . 0 . ^^^ The heme of cytochrome b 5 ( in the absence of b5R ) was also rapidly reduced in this system with k 2 = 3 . 1 10 10 ( 8 ) M 1 s 1 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Rat erythrocyte NADH cytochrome b 5 reductase . ^^^ The subcellular distribution of rat erythrocyte NADH cytochrome b 5 reductase was determined by radioimmunoassay , using a rabbit antibody against the cathepsin D cleaved water soluble fragment of rat liver microsomal reductase ( 1 reductase ) , which is known to be immunologically similar to the red cell enzyme . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
However , DEAE cellulose column chromatography of a detergent extract of microsomes yields a fraction that contains the oxidase , lipids , and NADH cytochrome b 5 reductase but is free of cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The delta 6 desaturase activity required NADH ( or NADPH ) , linoleoyl CoA , oxygen , lipid or detergent and three enzymes , such as NADH cytochrome b 5 reductase ( or NADPH cytochrome P 450 reductase ) , cytochrome b 5 , and delta 6 desaturase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The specific content of cytochrome b 5 was 16 . 5 nmol / mg protein and purified cytochrome b 5 fractions were free of cytochrome P 450 , NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase activities . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Fractionation and reconstitution experiments showed that this enzyme system consists of NADH cytochrome b 5 reductase , cytochrome b 5 and a 56 kDa terminal electron acceptor having the CMP Neu 5 Ac hydroxylase activity . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Under these conditions the total desaturase activity was found in the liposomal fraction thus indicating that the three components of the desaturase system , namely NADH cytochrome b 5 reductase , cytochrome b 5 and the delta 6 desaturase co extracted . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
A reconstitution experiment with the purified CMP NeuAc hydroxylase , soluble cytochrome b 5 , and recombinant NADH cytochrome b 5 reductase revealed that these three factors are essential for the reaction . ^^^ The molecular activity of the enzyme ( approximately 500 / min ) is much lower than that reported for NADH cytochrome b 5 reductase , suggesting that the activity or amount of hydroxylase is rate limiting in CMP N glycolylneuraminic acid ( NeuGc ) biosynthesis . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Stability and storage conditions of NADH cytochrome b 5 reductase cross linked into gelatin by chromium ( 3 ) acetate . ^^^ NADH cytochrome b 5 reductase was isolated and partially purified from rabbit liver microsomes . ^^^ Immobilized NADH cytochrome b 5 reductase activities were found to be stable for at least 72 d and 24 uses . ^^^ The storage stability of NADH cytochrome b 5 reductase was improved with immobilization at 25 degrees C . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Parameters investigated were : microsomal protein , NADPH cytochrome P 450 reductase activity , NADH cytochrome b 5 reductase activity , cytochrome b 5 content , cytochrome P 450 content , aminopyrine N demethylase activity , aniline p hydroxylase activity , p nitroanisole O demethylase activity , uridine diphosphate ( UDP ) glucuronyl transferase activity and glutathione S transferase activity . ^^^ By 48 h post mortem , NADPH cytochrome P 450 reductase activity decreased to 91 % , NADH cytochrome b 5 reductase activity decreased to 94 % , and cytochrome P 450 content decreased to 92 % of relative activities . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Anti b 5 antiserum inhibited the reduction of cytochrome b 5 by NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
These enzymes included DT diaphorase , NADH cytochrome b 5 reductase , and NADPH cytochrome P 450 reductase . ^^^ All the tumors subjected to enzyme profiling contained the bioreductive enzymes , DT diaphorase , NADH cytochrome b 5 reductase and NADPH cytochrome P 450 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The same treatment did not alter the liver microsomal cytochrome b 5 content , NADH cytochrome b 5 reductase and NADPH cytochrome P 450 reductase activities . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Ascorbate radicals competed with bound cytochrome b 5 for the reaction with reduced NADH cytochrome b 5 reductase . ^^^ Kinetic results show that the properties of NADH cytochrome b 5 reductase in thyroid microsomes were similar to those of the enzyme in liver microsomes . ^^^ Biochem . 108 , 804 810 ) , support the idea that ascorbate protects thyroid hormones from oxidative degradation through the NADH cytochrome b 5 reductase system . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Under physiological conditions , methemoglobin reduction is accomplished mainly by red cell NADH cytochrome b 5 reductase ( NADH methemoglobin reductase ) so efficiently that there is insignificant amounts of methemoglobin in the circulating blood . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Analysis of mutant NADH cytochrome b 5 reductase : apparent `` type 3 ' ' methemoglobinemia can be explained as type 1 with an unstable reductase . ^^^ In this patient , NADH cytochrome b 5 reductase deficiency was observed in lymphocytes and platelets as well as in erythrocytes , but this was not associated with mental retardation . ^^^ A base change was identified in the gene for NADH cytochrome b 5 reductase , causing an amino acid substitution from Leu 148 to Pro . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
However , cytochrome b 5 concentration and NADH cytochrome b 5 reductase activity were , respectively , 33 and 43 % higher than those of the control group at the same time . ^^^ When rats were fed the sunflower oil diet , the cytochrome P 450 concentration and NADH cytochrome b 5 reductase activity at 120 days were , respectively , 11 and 23 % lesser than those of control group . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Interaction of non myristoylated NADH cytochrome b 5 reductase with cytochrome b 5 dimyristoylphosphatidylcholine vesicles . ^^^ An expression vector for NADH cytochrome b 5 reductase containing a thrombin cleavage site directly before the N terminal glycine residue of the flavoprotein was used to isolate the non myristoylated enzyme by thrombin cleavage of the initial fusion protein of a short segment of the multiple cloning site of the plasmid vector and the reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The andien beta synthase activity was further increased from 2 . 5 to 6 times when NADH cytochrome b 5 reductase was added along with cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase and cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Purification and partial characterization of microsomal NADH cytochrome b 5 reductase from higher plant Catharanthus roseus . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
A Japanese man with cytochrome b 5 reductase ( b5R ) deficiency in various blood cell lineages ( red cells , platelets , and lymphocytes ) and in cultured fibroblasts demonstrated congenital methemoglobinemia associated with mental and neurological retardation , and various skeletal anomalies , such as spondylosis deformans and finger joint deformations , which have never been described in association with this enzyme deficiency . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase and cytochrome b 5 isoforms as models for the study of post translational targeting to the endoplasmic reticulum . ^^^ Cytochrome b 5 and NADH cytochrome b 5 reductase are integral membrane proteins with cytosolic active domains and short membrane anchors , which are inserted post translationally into their target membranes . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase in both age groups showed a 24 h rhythm , also the activity fluctuations of succinic dehydrogenase showed a tendency to 24 h rhythmicity , and the differences between minimal and maximal values were statistically insignificant . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The NADH dependent microsomal electron transfer system consists of NADH cytochrome b 5 reductase and cytochrome b 5 , which donates reducing equivalents to fatty acyl desaturase , cytochrome P 450 , and other reactions . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
This activity was also distinct from xanthine oxidoreductase and NADH cytochrome b 5 reductase , 2 other enzymes that catalyze metabolic activation of MMC , because the unique activity was not inhibited by allopurinol ( an inhibitor of xanthine oxidoreductase ) and its activity was the same with NADH and NADPH ( cytochrome b 5 reductase is specific to NADH ) . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Congenital methemoglobinemia caused by an erythrocytic deficiency of cytochrome b 5 reductase ( b5R ; type 1 ) in African American individuals was first reported by this laboratory . ^^^ A novel mutation found in the 3 ' domain of NADH cytochrome B 5 reductase in an African American family with type 1 congenital methemoglobinemia . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The reducing ability of iron chelates by NADH cytochrome B 5 reductase or cytochrome B 5 responsible for NADH supported lipid peroxidation . ^^^ The results showed the selectivity of NADH cytochrome b 5 reductase or cytochrome b 5 towards iron chelates and the subsequent ability of this system to promote peroxidation . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Interaction of ferric complexes with NADH cytochrome b 5 reductase and cytochrome b 5 : lipid peroxidation , H2O2 generation , and ferric reduction . ^^^ NADH dependent microsomal electron transfer involves the enzymes NADH cytochrome b 5 reductase and cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Yeast Mas70p and NADH cytochrome b 5 reductase are bitopic integral proteins of the mitochondrial outer membrane and are inserted into the lipid bilayer in an Nin Ccyto orientation via an NH 2 terminal signal anchor sequence . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The cytochrome P 450 and cytochrome b 5 contents , the NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase activities , aniline hydroxylase and 4 aminopyrine N demethylase activities were measured in the hepatic microsomal fraction . ^^^ In both sexes , the cholesterol diet decreased all the examined parameters with the exception of NADH cytochrome b 5 reductase activity . ^^^ The protein diet did not change the examined enzyme activities or levels with the exception of induction of NADH cytochrome b 5 reductase and 4 aminopyrine N demethylase activities . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
In an ascorbate supported system or a NADH supported reconstituted system containing NADH cytochrome b 5 reductase and cytochrome b 5 , menadione was not an inhibitor of lipid peroxidation at pH 7 . 5 , while some antioxidant ability was observed at lower pH ranges . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Hereditary methemoglobinemia due to reduced nicotinamide adenine dinucleotide ( NADH ) cytochrome b 5 reductase ( b5R ) deficiency is classified into two types , an erythrocyte ( type 1 ) and a generalized ( type 2 ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Electrostatic properties deduced from refined structures of NADH cytochrome b 5 reductase and the other flavin dependent reductases : pyridine nucleotide binding and interaction with an electron transfer partner . ^^^ Electrostatic properties on the protein surface were examined on the basis of the crystal structure of NADH cytochrome b 5 reductase refined to a crystallographic R factor of 0 . 223 at 2 . 1 A resolution and of the other three flavin dependent reductases . ^^^ A structural comparison of NADH cytochrome b 5 reductase with the other flavin dependent reductases , ferredoxin NADP+ reductase , phthalate dioxygenase reductase , and nitrate reductase , showed that the alpha / beta structure is the common motif for binding pyridine nucleotide . ^^^ The electrostatic potential of the surface near the flavin protruding side ( dimethylbenzene end of the flavin ring ) of NADH cytochrome b 5 reductase was positive over a wide area while that of the surface near the heme binding site of cytochrome b 5 was negative . ^^^ This implied that the flavin protruding side of NADH cytochrome b 5 reductase is suitable for interacting with its electron transfer partner , cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
As part of an ' enzyme directed ' approach to bioreductive drug development , we have measured the activity of NADH : cytochrome b 5 reductase ( B5R ) in human cancer cell lines in order to assess the role of this enzyme in activating bioreductive drugs , and thus in influencing the cytotoxicity of these compounds . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase ( b5R ) from the house fly was purified through solubilization of microsomes with Triton 10 100 followed by DEAE , carboxylmethyl and 5 ' ADP affinity column chromatography . ^^^ Both forms of purified b5R could reduce cytochrome b 5 and both could use NADH or NADPH as an electron donor , although NADH was more efficient . ^^^ NADH cytochrome b 5 reductase ( b5R ) from the house fly was purified through solubilization of microsomes with Triton 10 100 followed by DEAE , carboxylmethyl and 5 ' ADP affinity column chromatography . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
These results suggest that the guanabenz N hydroxylation is mediated via CYP1A2 , whereas the corresponding guanoxabenz N reduction is catalyzed by an enzyme system composed of cytochrome b 5 , NADH cytochrome b 5 reductase , and benzamidoxime reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Electrostatic interaction between NADH cytochrome b 5 reductase and cytochrome b 5 studied by site directed mutagenesis . ^^^ Electrostatic interaction between NADH cytochrome b 5 reductase and cytochrome b 5 was studied by site directed mutagenesis . ^^^ These results suggest that there should be other acidic residue ( s ) than group A in cytochrome b 5 which participate in binding with NADH cytochrome b 5 reductase . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH ( reduced Coenzyme 1 ) cytochrome b 5 reductase ( b5R ) is a multifunctional redox enzyme , whose deficiency causes recessive congenital methemoglobinemia . ^^^ Antibody based spot test for NADH cytochrome b 5 reductase activity for the laboratory diagnosis of congenital methemoglobinemia . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Proteolysis of intact and solubilized microsomes showed that the 50 kDa protein is resistant to fragmentation at the conditions which led to the removal of the membranous segments from cytochrome b 5 and the NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The neuroleptic flupenthixol reduces the expression in rats of the gene coding for NADH cytochrome b 5 reductase as measured by in situ hybridisation and its enzymic manifestation . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
A decrease in cytochrome b 5 content was accompanied by a compensatory increase in the activity of NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase ( b5R ) is a member of a flavoenzyme family of dehydrogenases electron transferases that participates in the transfer of electrons from the NADH generated in glycolysis to cytochrome b 5 . b5R is involved in the steady state reduction of methemoglobin to hemoglobin in erythrocytes and is also involved in lipid metabolism in other cell types . ^^^ A high frequency polymorphism of NADH cytochrome b 5 reductase in African Americans . ^^^ NADH cytochrome b 5 reductase ( b5R ) is a member of a flavoenzyme family of dehydrogenases electron transferases that participates in the transfer of electrons from the NADH generated in glycolysis to cytochrome b 5 . b5R is involved in the steady state reduction of methemoglobin to hemoglobin in erythrocytes and is also involved in lipid metabolism in other cell types . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Glycerol had no effect on the content of cytochrome b 5 or activities of NADPH cytochrome P 450 reductase or NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The participation of cytochrome b 5 and NADH cytochrome b 5 reductase in cytochrome P 450 dependent biotransformations has previously only been described for oxidative processes . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The ability of the purified sheep liver cytochrome b 5 to transfer electrons from NADH cytochrome b 5 reductase to cytochrome c was investigated . ^^^ NADH cytochrome b 5 reductase was also partially purified from the same source , detergent solubilized sheep liver microsomes , by using two successive DEAE cellulose , and 5 ' ADP agarose affinity column chromatographies . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
To investigate the general applicability of the proposed LTS , we fused the N terminus of E 3 to the N terminus of the NADH cytochrome b 5 reductase lacking the myristoyl group and N terminal 30 residue membrane anchor . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The ability of the purified b 5 1 and b 5 2 fractions to transfer the electrons from NADH cytochrome b 5 reductase to cytochrome c was investigated . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Cytochrome P 450 and cytochrome b 5 contents , NADPH cytochrome P 450 reductase , NADH cytochrome b 5 reductase , aniline hydroxylase and 4 aminopyrine N demethylase activities were measured in a hepatic microsomal fraction . ^^^ The only exception applied to induction of NADH cytochrome b 5 reductase and 4 aminopyrine N demethylase activities . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
It is assumed that ( 1 ) hypotonic treatment or digitonin causes disruption of the outer mitochondrial membrane , and , as a consequence , desorption of the membrane bound cytochrome c in a Mg2+ dependent fashion ; ( 2 ) incubation in isotonic KCI without Mg2+ results in swelling of mitochondrial matrix , disruption of the outer membrane and cytochrome c desorption whereas Mg2+ lowers the K+ permeability of the inner membrane and , hence , prevents swelling ; ( 3 ) desorbed cytochrome c is reduced by added NADH via NADH cytochrome b 5 reductase and cytochrome b 5 or by ascorbate and is oxidized by cytochrome oxidase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Thus , ubiquinone dependent antioxidant protection in +E +Se plasma membranes is based primarily on NADH cytochrome b 5 reductase , whereas additional protection needed in E Se plasma membranes is supported by the increase of ubiquinone levels , increased expression of the cytochrome b 5 reductase , and translocation of soluble DT diaphorase to the plasma membrane . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The following parameters were determined : cytochrome P 450 content , cytochrome b 5 content , NADPH cytochrome P 450 reductase activity , and NADH cytochrome b 5 reductase activity , succinate dehydrogenase ( SDH ) activity , and lactate dehydrogenase ( LDH ) activity . ^^^ NADH cytochrome b 5 reductase activity showed only slight deviations in individual age groups . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
We previously purified an NADH dependent Fe3+ chelate reductase ( NFR ) from maize roots with biochemical features of a cytochrome b 5 reductase ( b5R ) [ Sparla , Bagnaresi , Scagliarini and Trost ( 1997 ) FEBS Lett . 414 , 571 575 ] . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Systematic mutations of highly conserved His 49 and carboxyl terminal of recombinant porcine liver NADH cytochrome b 5 reductase solubilized domain . ^^^ The cDNA encoding solubilized porcine liver NADH cytochrome b 5 reductase catalytic domain ( Pb5R ) was cloned and overexpressed in Escherichia coli . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Extract treatment significantly increased the basal levels of acid soluble sulphydryl ( SH ) content , cytochrome P 450 , NADPH cytochrome P 450 reductase , cytochrome b 5 , NADH cytochrome b 5 reductase , glutathione S transferase , DT diaphorase , superoxide dismutase , catalase , glutathione peroxidase and glutathione reductase in the liver . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Plant cytochrome b 5 reductases ( b5R ) are assumed to be part of an ER associated redox chain that oxidizes NADH to provide electrons via cytochrome b 5 ( cyt b 5 ) to ER associated fatty acyl desaturase and related hydroxylases , as in mammalian cells . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Treatment of rats with DHEA caused an increase in the activity of the terminal desaturase of the stearoyl CoA desaturation system , without changing either the activity of NADH cytochrome b 5 reductase or the microsomal content of cytochrome b 5 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
We have identified a cytosolic cytochrome b type NAD ( P ) H oxidoreductase in mammals , a flavohemoprotein ( b5+b5R ) containing cytochrome b 5 ( b 5 ) and b 5 reductase ( b5R ) domains . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Biodiversity of the P 450 catalytic cycle : yeast cytochrome b5 / NADH cytochrome b 5 reductase complex efficiently drives the entire sterol 14 demethylation ( CYP 51 ) reaction . ^^^ The widely accepted catalytic cycle of cytochromes P 450 ( CYP ) involves the electron transfer from NADPH cytochrome P 450 reductase ( CPR ) , with a potential for second electron donation from the microsomal cytochrome b5 / NADH cytochrome b 5 reductase system . ^^^ Using purified proteins including Candida albicans CYP 51 and yeast NADPH cytochrome P 450 reductase , cytochrome b 5 and NADH cytochrome b 5 reductase , we show here that fungal CYP 51 mediated sterol 14alpha demethylation can be wholly and efficiently supported by the cytochrome b5 / NADH cytochrome b 5 reductase electron transport system . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
EE / LE inhibited not only the metabolic capacity of P 450 , a protein which directly undergoes suicide inhibition , but also the level of rat liver cytochrome b 5 ( dependent on the heme pool ) as well as the activities of NADPH cytochrome P 450 reductase and NADH cytochrome b 5 reductase in the liver and kidney . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Recessive congenital methemoglobinemia due to nicotinamide adenine dinucleotide ( NADH ) cytochrome b 5 reductase ( b5R ) deficiency is classified into 2 clinical types : type 1 ( erythrocyte type ) and type 2 ( generalized type ) . ^^^ A novel mutation in the NADH cytochrome b 5 reductase gene of a Chinese patient with recessive congenital methemoglobinemia . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Acquired methemoglobinemia is diagnosed by elevated methemoglobin with normal hemoglobin electrophoresis and normal NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The obtained results have shown an overall decrease of liver microsomal cytochrome P 450 , cytochrome b 5 , NADPH cytochrome P 450 reductase , NADH cytochrome b 5 reductase , and aniline hydroxylase with a subsequent decrease of phase 2 enzymes , glutathione S transferase and UDP glucuronyl transferase in cancer bearing animals . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Hereditary enzymopenic methemoglobinemia is a rare disease that predominantly results from defects in either the erythrocytic ( type 1 ) or microsomal ( type 2 ) forms of the enzyme NADH : cytochrome b 5 reductase ( EC 1 . 6 . 2 . 2 ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The activities of cytochrome b 5 ( cyt b 5 ) , NADH cytochrome b 5 reductase ( cyt b 5 R ) , glutathione S transferase ( GST ) , DT diaphorase ( DTD ) , glutathione peroxidase ( GPx ) , glutathione reductase ( GR ) , superoxide dismutase ( SOD ) and catalase ( CAT ) showed a significant increase in the liver at both dose levels of extract . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Significant increase in the activities of acid soluble sulfhydryl ( SH ) content , cytochrome P 450 , NADPH cytochrome P 450 reductase , cytochrome b 5 , NADH cytochrome b 5 reductase , glutathione S transferase ( GST ) , DT diaphorase ( DTD ) , superoxide dismutase ( SOD ) , catalase ( CAT ) , glutathione peroxidase ( GPx ) and glutathione reductase ( GR ) were observed in the liver at both dose levels of treatments . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Significant increases in the levels of acid soluble sulfhydryl ( SH ) content , NADPH cytochrome P 450 reductase , NADH cytochrome b 5 reductase , glutathione S transferase ( GST ) , DT diaphorase ( DTD ) , superoxide dismutase ( SOD ) , catalase , glutathione peroxidase ( GPX ) and glutathione reductase ( GR ) were observed in the liver . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase activity changes were insignificant . . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Cytochrome b 5 reductase ( b5R ) is an essential enzyme that exists in soluble and membrane bound isoforms , each with specific functions . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Effects of flavin binding motif amino acid mutations in the NADH cytochrome b 5 reductase catalytic domain on protein stability and catalysis . ^^^ Porcine NADH cytochrome b 5 reductase catalytic domain ( Pb5R ) has the RXY ( T / S ) + ( T / S ) flavin binding motif that is highly conserved among the structurally related family of flavoprotein reductases . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Familial idiopathic methemoglobinemia revisited : original cases reveal 2 novel mutations in NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Role of Thr ( 66 ) in porcine NADH cytochrome b 5 reductase in catalysis and control of the rate limiting step in electron transfer . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
A case of type 1 methaemoglobinaemia observed in a Polish subject with compound heterozygosity for two mutations in the reduced nicotinamide adenine dinucleotide ( NADH ) cytochrome b 5 reductase ( b5R ) gene is described . ^^^ Compound heterozygosity of two missense mutations in the NADH cytochrome b 5 reductase gene of a Polish patient with type 1 recessive congenital methaemoglobinaemia . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Hydrogen peroxide and cell density regulated expression of NADH cytochrome b 5 reductase in HeLa cells . ^^^ We have studied the effect of cell density and hydrogen peroxide on the expression of NADH cytochrome b 5 reductase in HeLa cells . ^^^ Polypeptide levels of the NADH cytochrome b 5 reductase increased about three fold in confluent HeLa cells compared to sparse cells . ^^^ The presence of pyruvate , a H2O2 scavenger , produced a significant increment ( 200 % ) in the levels of NADH cytochrome b 5 reductase in sparse cells , but less increase ( 25 % ) in confluent cells , suggesting that generation of endogenous H2O2 could repress NADH cytochrome b 5 reductase expression , particularly in sparse cultures . ^^^ Addition of tert butylhydroquinone , a compound which generates reactive oxygen species through redox cycling , also reduced expression of the NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Caveolin 1 alpha interacts with caveolin 1beta , caveolin 2 , actin , the microsomal form of NADH cytochrome B 5 reductase and ESA / reggie 1 / flotillin 2 as shown by co immunoprecipitation . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
NADH cytochrome b 5 reductase and cytochrome b 5 catalyze the microsomal reduction of xenobiotic hydroxylamines and amidoximes in humans . ^^^ We hypothesized that NADH cytochrome b 5 reductase and cytochrome b 5 were responsible for this enzymatic activity in humans . ^^^ Purified human soluble NADH cytochrome b 5 reductase and cytochrome b 5 , expressed in Escherichia coli , efficiently catalyzed the reduction of sulfamethoxazole hydroxylamine , dapsone hydroxylamine , and benzamidoxime , with apparent Km values similar to those found in human liver microsomes and specific activities ( Vmax ) 74 to 235 times higher than in microsomes . ^^^ In addition , polyclonal antibodies to both NADH cytochrome b 5 reductase and cytochrome b 5 significantly inhibited reduction activity for sulfamethoxazole hydroxylamine . ^^^ Finally , fibroblasts from a patient with type 2 hereditary methemoglobinemia ( deficient in NADH cytochrome b 5 reductase ) showed virtually no activity for hydroxylamine reduction , compared with normal fibroblasts . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Structure of human erythrocyte NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Prenatal diagnosis of recessive congenital methaemoglobinaemia type 2 : novel mutation in the NADH cytochrome b 5 reductase gene leading to stop codon read through . ^^^ A case of type 2 recessive congenital methaemoglobinaemia ( RCM ) observed in a Lebanese subject with a novel mutation in NADH cytochrome b 5 reductase gene is described . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Using these plasmids , the solubilized donain of porcine NADH cytochrome b 5 reductase was also highly accumulated on prevention of the translational initiation inhibition . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
For the N reduction of benzamidoxime an oxygen insensitive liver microsomal enzyme system that required cytochrome b 5 , NADH cytochrome b 5 reductase and a cytochrome P 450 isoenzyme of the subfamily 2D has been described . ^^^ Therefore it seemed reasonable to study the potential of cytochrome b 5 , NADH cytochrome b 5 reductase and CYP2D to detoxify these N hydroxylated metabolites by N reduction . ^^^ SMX HA and DDS N OH are reduced by cytochrome b 5 , NADH cytochrome b 5 reductase and CYP2D but also only by cytochrome b 5 and NADH cytochrome b 5 reductase without addition of CYP2D . ^^^ The reduction rate for SMX HA by cytochrome b 5 , NADH cytochrome b 5 reductase and CYP2D was 0 , 65 + / 0 , 1 nmol SMX / min / mg protein . ^^^ For DDS N OH the reduction rate by cytochrome b 5 , NADH cytochrome b 5 reductase and CYP2D was 1 . 79 + / 0 . 85 nmol DDS / min / mg protein and by cytochrome b 5 and NADH cytochrome b 5 reductase 1 . 25 + / 0 . 15 nmol DDS / min / mg protein . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Unusual dehydroxylation of antimicrobial amidoxime prodrugs by cytochrome b 5 and NADH cytochrome b 5 reductase . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Characterization of the second form of NADH cytochrome b 5 reductase gene from arachidonic acid producing fungus Mortierella alpina 1S 4 . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
Evaluation of data in terms of two dimensional random walk model : interaction between NADH cytochrome b 5 reductase and cytochrome b 5 . ^^^ As a result , the theoretical rate equation well accounting experimental findings was derived on the model of the liver microsomal NADH cytochrome b 5 reductase reaction . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
The erythrocyte membrane was separated into Triton 10 100 soluble and insoluble fractions , and the detergent soluble fraction was replaced by the soluble or membrane form of b5R ( NADH cytochrome b 5 reductase ) . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
It stimulated cytochrome b 5 at 48 and 96 h and NADH cytochrome b 5 reductase activity at 12 , 72 and 96 h . ^^^ Methanol , especially the lower dose , inhibited cytochrome P 450 after 48 h , but the higher methanol dose inhibited NADH cytochrome b 5 reductase activity in this time . ^^^ L carnitine , combined with the lower dose of methanol , stimulated NADPH cytochrome P 450 reductase after 48 h and cytochrome b 5 and NADH cytochrome b 5 reductase over the whole period of observation . ^^^
Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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Interacting proteins: P00167 and P00387	Pubmed	SVM Score :0.0
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