| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Cell cycle mechanisms of sister chromatid separation ; roles of Cut1 / separin and Cut2 / securin . ^^^ In this review , the roles of two proteins essential for the onset of anaphase in fission yeast , Cut2 / securin and Cut1 / separin , are discussed with regard to cell cycle regulation , and compared with the postulated roles of homologous proteins in other organisms . ^^^ Separin is a large protein ( MW approximately 180 kDa ) , the C terminus of which is conserved , and is thought to be inhibited by association with securin at the nonconserved N terminus . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| At the metaphase to anaphase transition , separin is activated by proteolysis of its inhibitory subunit ( securin ) called Pds 1 . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Separin activation coincides with securin destruction and partial separin cleavage , suggesting that several mechanisms regulate separin activity . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| During most of the cell cycle , separase is kept inactive by binding to an inhibitory protein called securin . ^^^ Separase activation requires proteolysis of securin , which is mediated by an ubiquitin protein ligase called the anaphase promoting complex . ^^^ Phosphorylation of separase recognition sites may be highly conserved and regulates sister chromatid separation independently of securin . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Mammalian orthologs of yeast Esp 1 and Pds 1 , separin and securin , have been identified [ 8 ] , and , as anticipated , a nondegradable mutant form of securin inhibits sister separation when added to mitotic Xenopus egg extracts [ 8 ] . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Securin binds and inhibits separase , a conserved cysteine endoprotease . ^^^ Proteolysis of securin at the metaphase to anaphase transition activates separase , which degrades a conserved cohesin subunit , thereby allowing sister chromatid separation . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Proteolysis of the securin Pdsl is necessary to liberate the separase Esp 1 , which then triggers sister chromatid separation , whereas inactivation of mitotic CDKs is a prerequisite for exit from mitosis and for starting a new round of DNA replication in the next cell cycle . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Spindle integrity during elongation is dependent on proteolysis of the securin Pds 1 but not on the activity of the separase Esp 1 . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Separase is inhibited by securin , which is degraded at the metaphase anaphase transition . ^^^ We show that separase is kept inactive under these conditions by a mechanism independent of binding to securin . ^^^ We propose that separase activation at the metaphase anaphase transition requires the removal of both securin and an inhibitory phosphate . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| A recent paper in Cell highlights a second mechanism of regulation of separase in addition to the bound inhibitor securin . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of the mitotic regulator Pds1 / securin by Cdc 28 is required for efficient nuclear localization of Esp1 / separase . ^^^ Separase is associated with its inhibitor , securin , until the time of anaphase initiation , when securin is degraded in an anaphase promoting complex / cyclosome ( APC / C ) dependent manner . ^^^ In budding yeast securin / Pds1 not only inhibits separase / Esp1 , but also promotes its nuclear localization . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| The dual mechanism of separase regulation by securin . ^^^ Separase is regulated by its binding partner securin in two ways : securin is required to support separase activity in anaphase ; and , at the same time , securin must be destroyed via ubiquitylation before separase becomes active . ^^^ The molecular mechanisms underlying this dual regulation of separase by securin are unknown . ^^^ RESULTS : We show that , in budding yeast , securin supports separase localization . ^^^ Separase enters the nucleus independently of securin , but securin is required and sufficient to cause accumulation of separase in the nucleus , where its known cleavage targets reside . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Regulation of human separase by securin binding and autocleavage . ^^^ Separase is activated by the degradation of its inhibitor securin and by the removal of inhibitory phosphates . ^^^ Inhibitor binding experiments in vitro reveal that securin prevents the access of substrate analogs to the active site of separase . ^^^ Upon securin degradation , the active site of full length separase becomes accessible , allowing rapid autocatalytic cleavage of separase at one of three sites . ^^^ Our data further indicate that securin inhibits separase by blocking the access of substrates to the active site of separase . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| In Drosophila , the securin PIM associates not only with separase ( SSE ) , but also with an additional protein , THR . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Prior to anaphase , separase is inhibited independently by affinity binding to securin and by specific inhibitory phosphorylation . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The separase securin complex is required for anaphase . ^^^ Separase activated by securin destruction cleaves the cohesin subunit Scc1 / Rad21 enriched in kinetochores . ^^^ Fission yeast Cut1 / separase resides in interphase cytoplasm and mobilizes to the spindle and the spindle pole bodies ( SPBs ) in mitosis , while Cut2 / securin remains in the nucleus from interphase to metaphase , and temporarily locates at the short spindle . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| The human securin is an anaphase inhibitor , preventing premature chromosome separation through inhibition of separase activity . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| In the budding yeast Saccharomyces cerevisiae , the degradation of the securin protein Pds 1 reverses the binding and inhibition of the separase protein Esp 1 . ^^^ IFY 1 accumulates in one cell arrested emb 30 / APC4 embryos and interacts with SEP 1 , a C . elegans separase , suggesting that IFY 1 functions as a C . elegans securin . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Human separase is a 230 kDa enzyme that is inhibited by binding to its protein inhibitor securin , specific phosphorylation , and subcellular localization . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| The activation of Separase requires prior degradation of its associated inhibitor , called securin . ^^^ After securin degradation , Separase cleaves the cohesins and triggers chromatid separation , a prerequisite for anaphase . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Here we show that homologue disjunction in mouse oocytes is dependent on proteolysis of the separase inhibitor securin and the Cdk 1 regulatory sub unit cyclin B 1 . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Moreover , sequence similarity among securin proteins , which associate as regulatory subunits with separase , is restricted to the signals that promote the mitotic degradation required for separase activation . ^^^ Here , we address the surprising divergence of separase and securin sequences . ^^^ The interactions of THR with separase and securin in Drosophila melanogaster are analogous to those of the human N terminal separase domain with its C terminal domain and securin . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Securin is an inhibitory protein that is bound to a protease called Separase to inhibit sister chromatid separation until the onset of anaphase . ^^^ At the metaphase to anaphase transition , Securin is degraded by the anaphase promoting complex or cyclosome , and Separase contributes to the release of cohesins from the chromosome , allowing for the segregation of sister chromatids to opposite spindle poles . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| This novel separase function is coregulated with its proteolytic activity by the separase inhibitor securin . ^^^ This helps to explain the coupling of anaphase and mitotic exit after securin degradation at anaphase onset , separase cleaves cohesin to trigger chromosome segregation and concurrently uses a non proteolytic mechanism to initiate mitotic exit . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| A brute force postgenome approach to identify temperature sensitive mutations that negatively interact with separase and securin plasmids . ^^^ BACKGROUND : The fission yeast Schizosaccharomyces pombe separase / Cut1 and securin / Cut2 are required for anaphase specific activation of proteolysis that leads to proper sister chromatid separation . ^^^ In addition , the manner of the inhibition by high copy securin and separase plasmids were quite different each other and did not result in the mitotic block . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Securin inhibits separase during the cell cycle until metaphase when it is degraded by the anaphase promoting complex / cyclosome ( APC / C ) . ^^^ Alternative pathways might therefore regulate separase and sister chromatid separation apart from proteolysis of the Drosophila securin PIM . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| In response to a single straying chromatid within a cell , a surveillance mechanism called the spindle checkpoint blocks the ubiquitin ligase activity of the anaphase promoting complex or cyclosome ( APC / C ) , stabilizes securin ( an APC / C substrate and an inhibitor of separase ) , and delays the activation of separase . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Here , we report that mutations in the gene three rows ( thr ) enhance , while mutations in pimples ( pim , encoding Drosophila Securin ) or separase ( Sse ) suppress , the sensitized phenotype . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| The separation of sister chromatids at the metaphase to anaphase transition is triggered by a protease called separase that is activated by the destruction of an inhibitory chaperone ( securin ) . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Separase is activated when its inhibitor , securin , is polyubiquitinated by the Cdc 20 regulated anaphase promoting complex ( APC ( Cdc 20 ) ) and consequently degraded . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| At anaphase , separase is activated by degradation of its inhibitory partner , securin . ^^^ Fission yeast securin ( Cut 2 ) has destruction boxes and a separase ( Cut 1 ) interaction site in the amino and carboxyl terminus , respectively . ^^^ Here we show that securin is essential for separase stability and also for proper repair of DNA damaged by ultraviolet , 10 ray and gamma ray irradiation . ^^^ Experiments using either mutant cohesin that can not be cleaved by separase or a protease dead separase provide evidence that this DNA repair function of securin separase acts through the cleavage of cohesin . ^^^ We propose that the securin separase complex might aid DNA repair by removing local cohesin in interphase cells . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| An interactive gene network for securin separase , condensin , cohesin , Dis1 / Mtc1 and histones constructed by mass transformation . ^^^ To obtain information on interactions among genes required for chromosome segregation , an approach called Strategy B was taken using mass transformation of the 1015 temperature sensitive ( ts ) mutants that were made by random mutagenesis and transformed by plasmids carrying the genes for securin , separase , condensin , cohesin , kinetochore microtubule binding proteins Dis1 / Mtc1 or histones . ^^^ For example , separase / Cut1 interacts with Cdc48 / p97 / VCP , which stabilizes securin and separase . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| This is accompanied by premature separase activation , and accumulation of securin in a novel modified form . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Spo 13 may also limit separase activity after the first division by ensuring securin reaccumulation and , in doing so , preventing precocious removal from chromatin of centromeric cohesin . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| At the metaphase to anaphase transition , separase is activated by proteolysis of securin . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Domain structure of separase and its binding to securin as determined by EM . ^^^ After the degradation of its inhibitor securin , separase initiates chromosome segregation during the metaphase to anaphase transition by cleaving cohesin . ^^^ Here we present a density map at a resolution of 25 A of negatively stained separase securin complex . ^^^ Based on labeling data and sequence analysis , we propose a model for the structure of separase , consisting of 26 ARM repeats , an unstructured region of 280 residues and two caspase like domains , with securin binding to the ARM repeats . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Destruction of mitotic cyclin and securin leads to mitotic exit and separase activation , respectively . ^^^ Key players in chromosome segregation are SMC containing cohesin and condensin , DNA topoisomerase 2 , APC / C ubiquitin ligase , securin separase complex , aurora passengers , and kinetochore microtubule destabilizers or regulators . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Anaphase is triggered when ring shaped cohesin is cleaved by separase , a protease regulated by association with its inhibitor securin . ^^^ Dispensability of vertebrate securin strongly suggests additional means of separase regulation . ^^^ Indeed , sister chromatid separation but not securin degradation is inhibited by constitutively active cyclin dependent kinase 1 ( Cdk 1 ) and can be rescued solely by preventing phosphorylation of separase . ^^^ This unanticipated function of separase is negatively regulated by securin but independent of separase ' s proteolytic activity . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Securin and separase phosphorylation act redundantly to maintain sister chromatid cohesion in mammalian cells . ^^^ A key target of the spindle assembly checkpoint is securin , the separase inhibitor . ^^^ We generated a nonphosphorylable point mutant ( S1121A ) separase allele in securin / mouse embryonic stem cells . ^^^ Securin ( / ) separase ( + / S1121A ) cells are viable but fail to maintain sister chromatid cohesion in response to the disruption of spindle microtubules , show enhanced sensitivity to nocodazole , and can not recover from prometaphase arrest . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Anaphase topsy turvy : Cdk 1 a securin , separase a CKI . ^^^ While it has been known for some time that the anaphase inhibitor securin regulates separase activity recent work shows that this is only half the story . ^^^ In vertebrates Cdk 1 dependent inhibition of separase represents a second , securin independent branch of anaphase regulation . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Separase activity is regulated by its inhibitor securin and by an inhibitory phosphorylation in vertebrates . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| We also demonstrated that heterozygous separase deficient cells exhibited severely restrained increases in cell number with apparently normal mitosis in the absence of securin , which is an inhibitory partner of separase . . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Separase is a protease whose liberation from its inhibitory chaperone Securin triggers sister chromatid disjunction at anaphase onset in yeast by cleaving cohesin ' s kleisin subunit . ^^^ We have created conditional knockout alleles of the mouse Separase and Securin genes . ^^^ Deletion of both copies of Separase but not Securin causes embryonic lethality . ^^^ Loss of Securin reduces Separase activity because deletion of just one copy of the Separase gene is lethal to embryos lacking Securin . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| For most of the cell cycle , separase activity is kept in check by its association with the inhibitory chaperone securin . ^^^ Activation of separase occurs at anaphase onset , when securin is targeted for destruction by the anaphase promoting complex or cyclosome E 3 ubiquitin protein ligase . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Also known as a human form of securin , PTTG is an anaphase inhibitor that prevents premature chromosome separation through inhibition of separase activity ; hence , its degradation is required to start anaphase . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Fission yeast MAP kinase is required for the increased securin separase interaction that rescues separase mutants under stresses . ^^^ Securin , the chaperon and inhibitor of separase , is destructed in anaphase after polyubiquitination , and resulting activated separase cleaves the cohesin subunit Scc1 / Rad21 . ^^^ Fission yeast securin / Cut2 and separase / Cut1 that form the complex are essential for viability and a number of temperature sensitive ( ts ) mutants have been isolated . ^^^ During the 2 3 hr arrested period that occurred prior to the restart of division cycle , the level of securin dramatically increased , apparently accompanying the increased complex formation with mutant separase protein . ^^^ Securin bound to separase was hyperphosphorylated . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Separase , the cysteine protease that cleaves cohesin and thereby triggers chromosome disjunction , is inhibited by both securin and phosphorylation dependent cyclin B 1 binding . ^^^ First , by elevating separase levels above securin , securin mediated inhibition is alleviated . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Securin can have a separase cleavage site by substitution mutations in the domain required for stabilization and inhibition of separase . ^^^ Securin separase complex is required for sister chromatid separation . ^^^ Separase is activated on the destruction of securin and cleaves cohesin . ^^^ Fission yeast securin / Cut2 required for proper separase localization has the motifs for destruction and separase binding at the N and C termini , respectively . ^^^ The fragment inhibits separase , while separase is recruited normally and securin is destroyed . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| Separase not only triggers anaphase of meiosis 1 by proteolytic cleavage of cohesin on chromosome arms , but in vitro vertebrate separase also acts as a direct inhibitor of cyclin dependent kinase 1 ( Cdk 1 ) on liberation from the inhibitory protein , securin . ^^^ |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95997 and Q14674 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Cell cycle mechanisms of sister chromatid separation ; roles of Cut1 / separin and Cut2 / securin . ^^^ In this review , the roles of two proteins essential for the onset of anaphase in fission yeast , Cut2 / securin and Cut1 / separin , are discussed with regard to cell cycle regulation , and compared with the postulated roles of homologous proteins in other organisms . ^^^ Separin is a large protein ( MW approximately 180 kDa ) , the C terminus of which is conserved , and is thought to be inhibited by association with securin at the nonconserved N terminus . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| At the metaphase to anaphase transition , separin is activated by proteolysis of its inhibitory subunit ( securin ) called Pds 1 . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Separin activation coincides with securin destruction and partial separin cleavage , suggesting that several mechanisms regulate separin activity . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Mammalian orthologs of yeast Esp 1 and Pds 1 , separin and securin , have been identified [ 8 ] , and , as anticipated , a nondegradable mutant form of securin inhibits sister separation when added to mitotic Xenopus egg extracts [ 8 ] . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Spindle integrity during elongation is dependent on proteolysis of the securin Pds 1 but not on the activity of the separase Esp 1 . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Separase is inhibited by securin , which is degraded at the metaphase anaphase transition . ^^^ We show that separase is kept inactive under these conditions by a mechanism independent of binding to securin . ^^^ We propose that separase activation at the metaphase anaphase transition requires the removal of both securin and an inhibitory phosphate . . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| A recent paper in Cell highlights a second mechanism of regulation of separase in addition to the bound inhibitor securin . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of the mitotic regulator Pds1 / securin by Cdc 28 is required for efficient nuclear localization of Esp1 / separase . ^^^ Separase is associated with its inhibitor , securin , until the time of anaphase initiation , when securin is degraded in an anaphase promoting complex / cyclosome ( APC / C ) dependent manner . ^^^ In budding yeast securin / Pds1 not only inhibits separase / Esp1 , but also promotes its nuclear localization . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| The dual mechanism of separase regulation by securin . ^^^ Separase is regulated by its binding partner securin in two ways : securin is required to support separase activity in anaphase ; and , at the same time , securin must be destroyed via ubiquitylation before separase becomes active . ^^^ The molecular mechanisms underlying this dual regulation of separase by securin are unknown . ^^^ RESULTS : We show that , in budding yeast , securin supports separase localization . ^^^ Separase enters the nucleus independently of securin , but securin is required and sufficient to cause accumulation of separase in the nucleus , where its known cleavage targets reside . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| During most of the cell cycle , separase is kept inactive by binding to an inhibitory protein called securin . ^^^ Separase activation requires proteolysis of securin , which is mediated by an ubiquitin protein ligase called the anaphase promoting complex . ^^^ Phosphorylation of separase recognition sites may be highly conserved and regulates sister chromatid separation independently of securin . . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Securin binds and inhibits separase , a conserved cysteine endoprotease . ^^^ Proteolysis of securin at the metaphase to anaphase transition activates separase , which degrades a conserved cohesin subunit , thereby allowing sister chromatid separation . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Proteolysis of the securin Pdsl is necessary to liberate the separase Esp 1 , which then triggers sister chromatid separation , whereas inactivation of mitotic CDKs is a prerequisite for exit from mitosis and for starting a new round of DNA replication in the next cell cycle . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| The human securin is an anaphase inhibitor , preventing premature chromosome separation through inhibition of separase activity . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| In the budding yeast Saccharomyces cerevisiae , the degradation of the securin protein Pds 1 reverses the binding and inhibition of the separase protein Esp 1 . ^^^ IFY 1 accumulates in one cell arrested emb 30 / APC4 embryos and interacts with SEP 1 , a C . elegans separase , suggesting that IFY 1 functions as a C . elegans securin . . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Human separase is a 230 kDa enzyme that is inhibited by binding to its protein inhibitor securin , specific phosphorylation , and subcellular localization . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| This novel separase function is coregulated with its proteolytic activity by the separase inhibitor securin . ^^^ This helps to explain the coupling of anaphase and mitotic exit after securin degradation at anaphase onset , separase cleaves cohesin to trigger chromosome segregation and concurrently uses a non proteolytic mechanism to initiate mitotic exit . . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| A brute force postgenome approach to identify temperature sensitive mutations that negatively interact with separase and securin plasmids . ^^^ BACKGROUND : The fission yeast Schizosaccharomyces pombe separase / Cut1 and securin / Cut2 are required for anaphase specific activation of proteolysis that leads to proper sister chromatid separation . ^^^ In addition , the manner of the inhibition by high copy securin and separase plasmids were quite different each other and did not result in the mitotic block . . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Regulation of human separase by securin binding and autocleavage . ^^^ Separase is activated by the degradation of its inhibitor securin and by the removal of inhibitory phosphates . ^^^ Inhibitor binding experiments in vitro reveal that securin prevents the access of substrate analogs to the active site of separase . ^^^ Upon securin degradation , the active site of full length separase becomes accessible , allowing rapid autocatalytic cleavage of separase at one of three sites . ^^^ Our data further indicate that securin inhibits separase by blocking the access of substrates to the active site of separase . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| In Drosophila , the securin PIM associates not only with separase ( SSE ) , but also with an additional protein , THR . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Prior to anaphase , separase is inhibited independently by affinity binding to securin and by specific inhibitory phosphorylation . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The separase securin complex is required for anaphase . ^^^ Separase activated by securin destruction cleaves the cohesin subunit Scc1 / Rad21 enriched in kinetochores . ^^^ Fission yeast Cut1 / separase resides in interphase cytoplasm and mobilizes to the spindle and the spindle pole bodies ( SPBs ) in mitosis , while Cut2 / securin remains in the nucleus from interphase to metaphase , and temporarily locates at the short spindle . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Securin inhibits separase during the cell cycle until metaphase when it is degraded by the anaphase promoting complex / cyclosome ( APC / C ) . ^^^ Alternative pathways might therefore regulate separase and sister chromatid separation apart from proteolysis of the Drosophila securin PIM . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| The activation of Separase requires prior degradation of its associated inhibitor , called securin . ^^^ After securin degradation , Separase cleaves the cohesins and triggers chromatid separation , a prerequisite for anaphase . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Here we show that homologue disjunction in mouse oocytes is dependent on proteolysis of the separase inhibitor securin and the Cdk 1 regulatory sub unit cyclin B 1 . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Moreover , sequence similarity among securin proteins , which associate as regulatory subunits with separase , is restricted to the signals that promote the mitotic degradation required for separase activation . ^^^ Here , we address the surprising divergence of separase and securin sequences . ^^^ The interactions of THR with separase and securin in Drosophila melanogaster are analogous to those of the human N terminal separase domain with its C terminal domain and securin . ^^^ |
|
| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Securin is an inhibitory protein that is bound to a protease called Separase to inhibit sister chromatid separation until the onset of anaphase . ^^^ At the metaphase to anaphase transition , Securin is degraded by the anaphase promoting complex or cyclosome , and Separase contributes to the release of cohesins from the chromosome , allowing for the segregation of sister chromatids to opposite spindle poles . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| At anaphase , separase is activated by degradation of its inhibitory partner , securin . ^^^ Fission yeast securin ( Cut 2 ) has destruction boxes and a separase ( Cut 1 ) interaction site in the amino and carboxyl terminus , respectively . ^^^ Here we show that securin is essential for separase stability and also for proper repair of DNA damaged by ultraviolet , 10 ray and gamma ray irradiation . ^^^ Experiments using either mutant cohesin that can not be cleaved by separase or a protease dead separase provide evidence that this DNA repair function of securin separase acts through the cleavage of cohesin . ^^^ We propose that the securin separase complex might aid DNA repair by removing local cohesin in interphase cells . . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| An interactive gene network for securin separase , condensin , cohesin , Dis1 / Mtc1 and histones constructed by mass transformation . ^^^ To obtain information on interactions among genes required for chromosome segregation , an approach called Strategy B was taken using mass transformation of the 1015 temperature sensitive ( ts ) mutants that were made by random mutagenesis and transformed by plasmids carrying the genes for securin , separase , condensin , cohesin , kinetochore microtubule binding proteins Dis1 / Mtc1 or histones . ^^^ For example , separase / Cut1 interacts with Cdc48 / p97 / VCP , which stabilizes securin and separase . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| This is accompanied by premature separase activation , and accumulation of securin in a novel modified form . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Spo 13 may also limit separase activity after the first division by ensuring securin reaccumulation and , in doing so , preventing precocious removal from chromatin of centromeric cohesin . . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| At the metaphase to anaphase transition , separase is activated by proteolysis of securin . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| In response to a single straying chromatid within a cell , a surveillance mechanism called the spindle checkpoint blocks the ubiquitin ligase activity of the anaphase promoting complex or cyclosome ( APC / C ) , stabilizes securin ( an APC / C substrate and an inhibitor of separase ) , and delays the activation of separase . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Here , we report that mutations in the gene three rows ( thr ) enhance , while mutations in pimples ( pim , encoding Drosophila Securin ) or separase ( Sse ) suppress , the sensitized phenotype . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| The separation of sister chromatids at the metaphase to anaphase transition is triggered by a protease called separase that is activated by the destruction of an inhibitory chaperone ( securin ) . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Separase is activated when its inhibitor , securin , is polyubiquitinated by the Cdc 20 regulated anaphase promoting complex ( APC ( Cdc 20 ) ) and consequently degraded . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Domain structure of separase and its binding to securin as determined by EM . ^^^ After the degradation of its inhibitor securin , separase initiates chromosome segregation during the metaphase to anaphase transition by cleaving cohesin . ^^^ Here we present a density map at a resolution of 25 A of negatively stained separase securin complex . ^^^ Based on labeling data and sequence analysis , we propose a model for the structure of separase , consisting of 26 ARM repeats , an unstructured region of 280 residues and two caspase like domains , with securin binding to the ARM repeats . . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Destruction of mitotic cyclin and securin leads to mitotic exit and separase activation , respectively . ^^^ Key players in chromosome segregation are SMC containing cohesin and condensin , DNA topoisomerase 2 , APC / C ubiquitin ligase , securin separase complex , aurora passengers , and kinetochore microtubule destabilizers or regulators . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Anaphase is triggered when ring shaped cohesin is cleaved by separase , a protease regulated by association with its inhibitor securin . ^^^ Dispensability of vertebrate securin strongly suggests additional means of separase regulation . ^^^ Indeed , sister chromatid separation but not securin degradation is inhibited by constitutively active cyclin dependent kinase 1 ( Cdk 1 ) and can be rescued solely by preventing phosphorylation of separase . ^^^ This unanticipated function of separase is negatively regulated by securin but independent of separase ' s proteolytic activity . . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Securin and separase phosphorylation act redundantly to maintain sister chromatid cohesion in mammalian cells . ^^^ A key target of the spindle assembly checkpoint is securin , the separase inhibitor . ^^^ We generated a nonphosphorylable point mutant ( S1121A ) separase allele in securin / mouse embryonic stem cells . ^^^ Securin ( / ) separase ( + / S1121A ) cells are viable but fail to maintain sister chromatid cohesion in response to the disruption of spindle microtubules , show enhanced sensitivity to nocodazole , and can not recover from prometaphase arrest . . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Anaphase topsy turvy : Cdk 1 a securin , separase a CKI . ^^^ While it has been known for some time that the anaphase inhibitor securin regulates separase activity recent work shows that this is only half the story . ^^^ In vertebrates Cdk 1 dependent inhibition of separase represents a second , securin independent branch of anaphase regulation . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Separase activity is regulated by its inhibitor securin and by an inhibitory phosphorylation in vertebrates . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Securin can have a separase cleavage site by substitution mutations in the domain required for stabilization and inhibition of separase . ^^^ Securin separase complex is required for sister chromatid separation . ^^^ Separase is activated on the destruction of securin and cleaves cohesin . ^^^ Fission yeast securin / Cut2 required for proper separase localization has the motifs for destruction and separase binding at the N and C termini , respectively . ^^^ The fragment inhibits separase , while separase is recruited normally and securin is destroyed . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| We also demonstrated that heterozygous separase deficient cells exhibited severely restrained increases in cell number with apparently normal mitosis in the absence of securin , which is an inhibitory partner of separase . . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Separase is a protease whose liberation from its inhibitory chaperone Securin triggers sister chromatid disjunction at anaphase onset in yeast by cleaving cohesin ' s kleisin subunit . ^^^ We have created conditional knockout alleles of the mouse Separase and Securin genes . ^^^ Deletion of both copies of Separase but not Securin causes embryonic lethality . ^^^ Loss of Securin reduces Separase activity because deletion of just one copy of the Separase gene is lethal to embryos lacking Securin . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| For most of the cell cycle , separase activity is kept in check by its association with the inhibitory chaperone securin . ^^^ Activation of separase occurs at anaphase onset , when securin is targeted for destruction by the anaphase promoting complex or cyclosome E 3 ubiquitin protein ligase . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Separase , the cysteine protease that cleaves cohesin and thereby triggers chromosome disjunction , is inhibited by both securin and phosphorylation dependent cyclin B 1 binding . ^^^ First , by elevating separase levels above securin , securin mediated inhibition is alleviated . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Separase not only triggers anaphase of meiosis 1 by proteolytic cleavage of cohesin on chromosome arms , but in vitro vertebrate separase also acts as a direct inhibitor of cyclin dependent kinase 1 ( Cdk 1 ) on liberation from the inhibitory protein , securin . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Also known as a human form of securin , PTTG is an anaphase inhibitor that prevents premature chromosome separation through inhibition of separase activity ; hence , its degradation is required to start anaphase . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| Fission yeast MAP kinase is required for the increased securin separase interaction that rescues separase mutants under stresses . ^^^ Securin , the chaperon and inhibitor of separase , is destructed in anaphase after polyubiquitination , and resulting activated separase cleaves the cohesin subunit Scc1 / Rad21 . ^^^ Fission yeast securin / Cut2 and separase / Cut1 that form the complex are essential for viability and a number of temperature sensitive ( ts ) mutants have been isolated . ^^^ During the 2 3 hr arrested period that occurred prior to the restart of division cycle , the level of securin dramatically increased , apparently accompanying the increased complex formation with mutant separase protein . ^^^ Securin bound to separase was hyperphosphorylated . ^^^ |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q14674 and O95997 |
Pubmed |
SVM Score :0.0 |
| NA |
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