| Interacting proteins: O95997 and P53801 |
Pubmed |
SVM Score :0.88682141 |
| Glutathione S transferase pull down and co immunoprecipitation assays demonstrate that PBF interacts specifically with PTTG under both in vitro and in vivo conditions . 0.88682141^^^ |
|
| Interacting proteins: O95997 and P53801 |
Pubmed |
SVM Score :0.0 |
| Several of these events are mediated through interactions with PTTG binding factor ( PBF ) and fibroblast growth factor 2 ( FGF 2 ) . ^^^ Given this background , we have determined the expression of PTTG , PBF , FGF 2 and its receptor FGF R 1 in a large cohort of pituitary adenomas and have sought associations between levels of gene expression and clinical markers of tumour behaviour . ^^^ PATIENTS AND METHODS : We used real time reverse transcriptase polymerase chain reaction ( RT PCR ) and Western blot analyses to measure PTTG , PBF , FGF 2 and FGF R 1 expression in ex vivo pituitary tumours ( N = 121 ) . ^^^ We also demonstrated that PBF was similarly raised in adenomas ( sixfold , P = 0 . 0001 ) , and was significantly correlated with PTTG expression . ^^^ |
|
| Interacting proteins: O95997 and P53801 |
Pubmed |
SVM Score :0.0 |
| FGF 2 protein was concordantly lower in fetal cortex , whereas pretranslational expression of PTTG binding factor ( PBF ) was not significantly altered in fetal brain compared with adult . ^^^ |
|
| Interacting proteins: O95997 and P53801 |
Pubmed |
SVM Score :0.0 |
| Here we report expressions of PTTG and its interacting protein , PTTG binding factor in human astrocytic cells . ^^^ PTTG expression was higher in malignant cells than in primary astrocytes , whereas PTTG binding factor was not . ^^^ |
|
| Interacting proteins: O95997 and P53801 |
Pubmed |
SVM Score :0.0 |
| The Pttg1ip promoter binds Runx 2 in a sequence specific manner , and Runx 2 is able to transactivate the Pttg lip promoter in MC3T3 El cells . ^^^ |
|
| Interacting proteins: O95997 and P53801 |
Pubmed |
SVM Score :0.0 |
| Clinical studies reveal that PTTG binding factor , fibroblast growth factor 2 , and vascular endothelial growth factor are elevated in pituitary tumors , and mostly correlate with PTTG levels , also confirming the PTTG role in angiogenesis . ^^^ |
|
| Interacting proteins: O95997 and P53801 |
Pubmed |
SVM Score :0.0 |
| Pituitary tumor transforming gene ( PTTG ) is a protooncogene implicated in the pathogenesis of multiple tumor types , which stimulates fibroblast growth factor 2 secretion via PTTG binding factor ( PBF ) . ^^^ PTTG was able to up regulate PBF mRNA expression in vitro ( P < 0 . 001 ; n = 12 ) , and stable overexpression of PBF in NIH3T3 cells resulted in significant colony formation ( P < 0 . 001 ; n = 12 ) . ^^^ |
|
| Interacting proteins: P53801 and O95997 |
Pubmed |
SVM Score :0.88682141 |
| Glutathione S transferase pull down and co immunoprecipitation assays demonstrate that PBF interacts specifically with PTTG under both in vitro and in vivo conditions . 0.88682141^^^ |
|
| Interacting proteins: P53801 and O95997 |
Pubmed |
SVM Score :0.0 |
| Several of these events are mediated through interactions with PTTG binding factor ( PBF ) and fibroblast growth factor 2 ( FGF 2 ) . ^^^ Given this background , we have determined the expression of PTTG , PBF , FGF 2 and its receptor FGF R 1 in a large cohort of pituitary adenomas and have sought associations between levels of gene expression and clinical markers of tumour behaviour . ^^^ PATIENTS AND METHODS : We used real time reverse transcriptase polymerase chain reaction ( RT PCR ) and Western blot analyses to measure PTTG , PBF , FGF 2 and FGF R 1 expression in ex vivo pituitary tumours ( N = 121 ) . ^^^ We also demonstrated that PBF was similarly raised in adenomas ( sixfold , P = 0 . 0001 ) , and was significantly correlated with PTTG expression . ^^^ |
|
| Interacting proteins: P53801 and O95997 |
Pubmed |
SVM Score :0.0 |
| FGF 2 protein was concordantly lower in fetal cortex , whereas pretranslational expression of PTTG binding factor ( PBF ) was not significantly altered in fetal brain compared with adult . ^^^ |
|
| Interacting proteins: P53801 and O95997 |
Pubmed |
SVM Score :0.0 |
| Pituitary tumor transforming gene ( PTTG ) is a protooncogene implicated in the pathogenesis of multiple tumor types , which stimulates fibroblast growth factor 2 secretion via PTTG binding factor ( PBF ) . ^^^ PTTG was able to up regulate PBF mRNA expression in vitro ( P < 0 . 001 ; n = 12 ) , and stable overexpression of PBF in NIH3T3 cells resulted in significant colony formation ( P < 0 . 001 ; n = 12 ) . ^^^ |
|
| Interacting proteins: P53801 and O95997 |
Pubmed |
SVM Score :0.0 |
| Clinical studies reveal that PTTG binding factor , fibroblast growth factor 2 , and vascular endothelial growth factor are elevated in pituitary tumors , and mostly correlate with PTTG levels , also confirming the PTTG role in angiogenesis . ^^^ |
|
| Interacting proteins: P53801 and O95997 |
Pubmed |
SVM Score :0.0 |
| The Pttg1ip promoter binds Runx 2 in a sequence specific manner , and Runx 2 is able to transactivate the Pttg lip promoter in MC3T3 El cells . ^^^ |
|
| Interacting proteins: P53801 and O95997 |
Pubmed |
SVM Score :0.0 |
| Here we report expressions of PTTG and its interacting protein , PTTG binding factor in human astrocytic cells . ^^^ PTTG expression was higher in malignant cells than in primary astrocytes , whereas PTTG binding factor was not . ^^^ |
|