Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.61491328
The proximity of the myc tag to the COOH terminal YV sequence of claudin 1 appeared to interfere with its interaction with ZO 1 , since over expression of non tagged claudin 1 increased TER but had a minimal effect on solute flux and no aberrant strands formed . 0.61491328^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
We herein show that induction of HNF 4alpha initiates differentiation of F 9 cells to polarized epithelial cells , in which tight junction proteins occludin , claudin 6 , claudin 7 , and ZO 1 are concentrated at the apical most regions of lateral membranes . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Occludin , ZO 1 , JAM 1 , and claudin 1 , 3 , 4 , and 14 , but not claudin 7 , mRNAs were decreased in recurrent tonsillitis compared with those of tonsillar hypertrophy . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Similar results were obtained from IHC of the airway epithelium and some renal tubules ; however , claudin 7 did partly colocalize with ZO 1 in EPH 4 cells , a normal murine mammary cell line , and in the epididymis . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Overexpression of claudin 7 did not affect the expression and localization of endogenous claudin 1 , 3 , 4 , 7 , and ZO 1 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
In the present study , we confirmed the expressions of claudin 1 , claudin 7 , and ZO 1 in the prickle cell layer of the normal human esophageal squamous epithelium . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
ZO 1 , ZO 2 , and ZO 3 bound to the COOH terminal YV sequence of claudin 1 to 8 through their PDZ 1 domains in vitro . ^^^ Then , claudin 1 or 2 was transfected into L fibroblasts , which express ZO 1 but not ZO 2 or ZO 3 . ^^^ Claudin 1 and 2 were concentrated at cell cell borders in an elaborate network pattern , to which endogenous ZO 1 was recruited . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The myc tagged claudin 1 precisely colocalized with both occludin and ZO 1 at cell cell contact sites , indicating that exogenous claudin 1 was properly targeted to the TJs . ^^^ Immunoblot analysis revealed that overexpression of claudin 1 increased expression of ZO 1 but not of occludin or ZO 2 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The expression of occludin and claudin 1 was downregulated , and the distribution patterns of ZO 1 and E cadherin were altered . ^^^ In addition , the presence of exogenous occludin protein led to a recovery in claudin 1 protein level , relocation of the zonula occludens 1 protein ( ZO 1 ) to the TJ , and redistribution of E cadherin to the lateral membrane . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
In Ras transformed Madin Darby canine kidney cells , occludin , claudin 1 , and ZO 1 were absent from cell cell contacts but were present in the cytoplasm , and the adherens junction protein E cadherin was weakly expressed . ^^^ After treatment of the Ras transformed cells with the mitogen activated protein kinase kinase ( MEK 1 ) inhibitor PD 98059 , which blocks the activation of mitogen activated protein kinase ( MAPK ) , occludin , claudin 1 , and ZO 1 were recruited to the cell membrane , tight junctions were assembled , and E cadherin protein expression was induced . ^^^ We also found that during the process of tight junction assembly after MEK 1 inhibition , tyrosine phosphorylation of occludin and ZO 1 , but not claudin 1 , increased significantly . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
In addition to morphological analysis , tumors were screened for the expression of junctional proteins , such as occludin , claudin 1 , ZO 1 and catenins . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Distribution of tight junction proteins claudin 1 , occludin , and ZO 1 was significantly altered during infection . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Rat and chicken brain endothelial cells form tight junctions which are positive for claudin 1 , claudin 5 , occludin and ZO 1 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
PURPOSE : To investigate the expression and cellular distribution of putative tight junction ( TJ ) proteins occludin , ZO 1 , ZO 2 , and claudin 1 in rat corneal epithelium and alterations of TJs in cultured human corneal epithelial cells in response to lipopolysaccharide ( LPS ) challenge . ^^^ METHODS : Immunohistochemistry was used to determine tissue distribution of occludin , ZO 1 , ZO 2 , and claudin 1 in the rat cornea . ^^^ RESULTS : Occludin , ZO 1 , and ZO 2 were found at the cell borders of the superficial layer , whereas claudin 1 was localized mainly in the basal and wing cell layers of rat corneal epithelium . ^^^ CONCLUSIONS : Occludin , ZO 1 , and ZO 2 , but not claudin 1 , are components of corneal epithelial TJs . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
However , the expression of the tight junction molecules ZO 1 , occludin , claudin 1 and claudin 5 was not changed in capillaries of SHRSP . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
IL 15 mediated up regulation of ZO 1 and ZO 2 expression was independent of the IL 2Rbeta subunit , whereas the phosphorylation of occludin and enhanced membrane association of claudin 1 and claudin 2 by IL 15 required the presence of the IL 2Rbeta subunit . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Using the yeast two hybrid system , we identified ZO 1 and MUPP 1 ( multi PDZ domain protein 1 ) as binding partners for the COOH terminus of claudin 1 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Immunolocalizations of TJ proteins , carried out in conditions similar to the electrophysiological experiments , show a very nice correlation between ZO 1 and claudin 1 localizations and G alterations induced by Ca++ removal from the basolateral solution , both in the absence and presence of H7 . . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Expression of epithelial intercellular TJ proteins ( occludin , ZO 1 , claudin 1 , and JAM ) and subjacent AJ ( beta catenin and E cadherin ) proteins were examined by immunoflourescence / confocal microscopy , immunohistochemistry , and Western blotting . ^^^ In contrast , expression of other TJ and AJ proteins such as ZO 1 , claudin 1 , JAM , beta catenin , and E cadherin were down regulated only in epithelial cells immediately adjacent to transmigrating PMN . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Exogenously expressed JEAP co localized with ZO 1 and occludin at TJs in polarized Madin Darby canine kidney cells , but not with claudin 1 , JAM , or ZO 1 in L cells . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Formation of tight junction strands by expression of claudin 1 mutants in their ZO 1 binding site in MDCK cells . ^^^ The differences between these constructs are that a putative ZO 1 binding sequence ( KDYV ) at the COOH terminus of claudin 1 was deleted ( DeltaCmyc ) or present ( 1CLmyc and DeltaCmycYV ) , or that a myc epitope was added at the COOH terminus ( 1CLmyc and DeltaCmyc ) or inserted just before the KDYV sequence ( DeltaCmycYV ) . ^^^ These results suggest that , although the addition of the myc epitope at or near the COOH terminus of claudin 1 interfered with the binding to ZO 1 and induced aberrant TJ strand formation , endogenous claudins which could bind to ZO 1 might recruit these deformed claudin 1s expressed at a low level to apical TJs . ^^^ A calcium switch assay revealed that claudin 1 was transported to cadherin based cell cell contacts where ZO 1 had already accumulated , and was then concentrated at apical TJs together with ZO 1 . ^^^ Crosslinking between claudin 1 and the perijunctional actin ring through ZO 1 may be necessary for TJ strands to be localized or retained at apical TJs . . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Both integral ( claudin 1 and occludin ) and membrane associated zonula occluden 1 and 2 ( ZO 1 and ZO 2 ) proteins combine to form these TJ complexes that are anchored to the cytoskeletal architecture ( actin ) . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Alterations were not perceptible at the level of choroidal tight junctions , as shown by freeze fracture , claudin 1 and ZO 1 immunolocalizations and conventional electron microscopy , after intravenous injection of cytochrome C . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
In stable wild type Cx 32 transfectants , occludin , claudin 1 , and ZO 1 mRNAs and proteins were significantly increased compared to parental cells and all of the proteins were colocalized with Cx 32 at cell borders . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Colocalization was analyzed for pairs of the following proteins : ZO 1 , occludin , claudin 1 , E cadherin and F actin . ^^^ Under EGTA treatment , colocalization for ZO 1 / occludin , ZO 1 / claudin 1 , claudin 1 / occludin , E cadherin / occludin and E cadherin / claudin 1 dropped below 35 % of the control value . ^^^ During the opening of TJs and AJs , ZO 1 colocalized with E cadherin in the lateral membrane region , whereas in controls , ZO 1 colocalized with occludin and claudin 1 in the junctional complex . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
This increase is not reflected in variations of the linear amount of TJ nor in changes of the pattern of junctional strands as observed in freeze fracture replicas , nor in the distribution of claudin 1 ( a membrane protein of the TJ ) nor ZO 1 ( a TJ associated protein ) . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Enhanced PP2A activity induces dephosphorylation of the TJ proteins , ZO 1 , occludin , and claudin 1 , and is associated with increased paracellular permeability . ^^^ Conversely , inhibition of PP2A by okadaic acid promotes the phosphorylation and recruitment of ZO 1 , occludin , and claudin 1 to the TJ during junctional biogenesis . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
We measured the effects of cytokine exposure of CF and non CF well differentiated primary human airway epithelial cells on TJ properties , including transepithelial resistance , paracellular permeability to hydrophilic solutes , and the TJ proteins occludin , claudin 1 , claudin 4 , junctional adhesion molecule , and ZO 1 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
By immunocytochemistry we have localized claudin 1 , occludin and protein ZO 1 in distinct plasma membrane structures representing cell cell attachment zones . ^^^ While claudin 1 occurs in plasma membranes of all living cell layers , protein ZO 1 is concentrated in or even restricted to the uppermost layers , and occludin is often detected only in the stratum granulosum . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Distribution patterns of the tight junction associated proteins ZO 1 , claudin 1 and occludin were investigated in rat uterine epithelial cells during early pregnancy . ^^^ Our results show that ZO 1 , claudin 1 and occludin are present in the apical region of uterine epithelial cells , and appear to play a role in the very dynamic tight junctional network of uterine epithelial cells during early pregnancy . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Western blotting revealed that HGF / SF decreased the protein level , and increased tyrosine phosphorylation of ZO 1 , but did not cause a change in level of occludin or claudin 1 , both molecules involved in tight junction function . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
In the present work we demonstrate that heterotypic TJs in mixed monolayers of MDCK cells with a different cell type ( LLC PK 1 ) are true TJs through several criteria , such as TER , the ability to stop the membrane diffusion of fluorescent sphingomyelin from the apical to the lateral domain , the presence of ZO 1 , ZO 2 , occludin , claudin 1 and claudin 2 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Immunofluorescent labelling of frozen liver sections from DNBS IL10KO mice , immunolocalization for and claudin 1 and ZO 1 resulted in a significant alteration in the localization of the immunosignals for claudin 1 and ZO 1 after DNBS administration in comparison with DNBS IL10WT . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
In contrast to results obtained with a control kidney cell line , MDCK 2 , HSG cells were unable to synthesize four important tight junction associated proteins : ZO 1 , occludin , claudin 1 , and claudin 2 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
However , immunofluorescence microscopy of drug treated cells showed that the localization and staining pattern of tight junction proteins ZO 1 , occludin , and claudin 1 , or the actin myosin ring at the zonula adherens , were not modified . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Small t induced TJ defects are associated with a loss of PP2A from areas of cell cell contact ; altered distribution and reduced amounts of the TJ proteins ZO 1 , occludin , and claudin 1 ; and marked disorganization of the actin cytoskeleton . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Incubation of cells with DETA NONOate or cytomix decreased epithelial barrier function , decreased expression of ZO 1 mRNA , decreased expression of ZO 1 , ZO 3 , and occludin protein , and increased expression of claudin 1 protein . ^^^ Cytomix caused incorrect subcellular localization of ZO 1 , occludin , and claudin 1 , and this was modulated by co incubation with cPTIO . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
We also performed immunostaining and immunoblotting for TJ associated proteins claudin 1 and 3 , occludin , zonula occluden 1 ( ZO 1 ) and 7H6 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Immunofluorescence of intercellular junction proteins ( tight junctions : ZO 1 , occludin , and claudin 1 ; adherens junctions : beta catenin ) became weak and uneven after culture with glucose . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
To this purpose , we used two tight junction markers , the Zonula occludens ( ZO 1 ) and claudin 1 proteins , and a glial marker , the aquaporin 4 ( AQP 4 ) protein , whose expression is correlated with BBB differentiation and integrity . ^^^ ZO 1 immunocytochemistry and claudin 1 immunofluorescence in mdx mice revealed a diffuse staining of microvessels as compared with the control ones , which displayed a banded staining pattern . ^^^ These findings indicate that dystrophin deficiency in the mdx brain leads to severe injury of the endothelial and glial cells with disturbance in alpha actin cytoskeleton , ZO 1 , claudin 1 , and AQP 4 assembly , as well as BBB breakdown . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Expression of the various genes throughout human preimplantation development showed differing levels of sensitivity of detection ; these genes included claudin 1 , occludin ( TM4+ and TM 4 isoforms ) , ZO 1 ( ZO 1alpha+ and ZO 1alpha isoforms ) , ZO 2 and JAM ( junction adhesion molecule ) , and the desmosome junction gene , DSC 2 ( desmocollin 2 ) . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
METHODS : Light and electron immunohistochemistry was used to determine tissue distribution of occludin , claudin 1 and ZO 1 in the human corneal epithelium . ^^^ In en face sections , claudin 1 and ZO 1 antibodies showed as bands that corresponded to the junctional complex . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
It was further revealed that protein and mRNA levels of occludin , but not claudin 1 and 5 , and ZO 1 , were reduced by 17 beta estradiol , in line with changes of TER . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Recently it was reported that in Ha Ras overexpressing MDCK cells the tight junction proteins claudin 1 , occludin and ZO 1 were absent at cell cell contact sites but present in the cytoplasm . ^^^ In order to evaluate the relevance of the MEK 1 pathway in tight junction regulation in breast cancer cells , we investigated the effect ofMEK 1 inhibition on expression of claudin 1 , occludin and ZO 1 in natively claudin 1 expressing T 47 D cells ( low Ras activity ) , claudin 1 negative MCF 7 cells ( elevated Ras activity ) as well as two retroviral claudin 1 transduced MCF 7 daughter cell lines with prominent membrane and cytoplasmic claudin 1 dominant homing , respectively . ^^^ Although we effectively blocked phosphorylation of MAPKs ERK 1 and ERK 2 using the selective MEK 1 inhibitor PD 98059 , no quantitative changes of mRNA or protein levels of claudin 1 , occludin and ZO 1 could be detected in all cell lines investigated . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
In contrast , a significant reduced alteration in the localization of the immunosignals for claudin 1 and ZO 1 was observed in the liver from iNOSKO mice after DNBS administration . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
A 24 hr exposure of BMEC to Tat ( 1 72 ) resulted in a decrease of claudin 1 , claudin 5 , and zonula occludens ( ZO ) 2 expression , whereas total levels of occludin and ZO 1 remained unchanged . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
We showed here that cAMP increased gene expression of claudin 5 and decreased that of claudin 1 in porcine blood brain barrier endothelial cells via protein kinase A ( PKA ) independent and dependent pathways , respectively . cAMP also enhanced immunoreactivity of claudin 5 along cell borders and in the cytoplasm , reorganized actin filaments , and altered signals of claudin 5 , occludin , ZO 1 , and ZO 2 along cell boundaries from zipperlike to linear patterns . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
End3 cells of mRNA transcripts for the following transporters : GLUT 1 ; MCT 1 and 2 ; OAT 1 ; Oatp 1 ; mdr 1a and 1b ; MRP 1 and 5 ; beta alanine , system L and system y+L amino acid carriers ; the nucleoside transporters cNT 1 and 2 , eNT 1 and 2 , and the tight junctional elements , ZO 1 , JAM , occludin , claudin 1 and 5 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Aberrant expression of tight junction related proteins ZO 1 , claudin 1 and occludin in synovial sarcoma : an immunohistochemical study with ultrastructural correlation . ^^^ The tight junction is a multiprotein complex consisting of numerous proteins that include ZO 1 , claudin 1 and occludin . ^^^ A total of 35 cases of synovial sarcoma ( 13 biphasic , 14 monophasic and eight poorly differentiated ) were immunostained for ZO 1 , claudin 1 and occludin using commercially available antibodies , heat induced epitope retrieval and standard avidin biotin technique . ^^^ The glands of biphasic synovial sarcomas expressed ZO 1 ( 13 / 13 ) , claudin 1 ( 12 / 13 ) and occludin ( 11 / 13 ) in a manner identical to normal glandular epithelia , at the apical portion of the lateral membrane . ^^^ The spindle cells of biphasic synovial sarcomas showed abnormal circumferential membranous expression of ZO 1 ( 12 / 13 ) , claudin 1 ( 6 / 13 ) and occludin ( 3 / 13 ) . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Duplicate cells were lysed and ZO 1 , occludin , and claudin 1 amount determined by western blot . ^^^ CONCLUSIONS : ( 1 ) MDCK TJs are qualitatively disrupted by TNFalpha . ( 2 ) This disruption is not because of a decrease in cell number , lack of cell layer confluency , or a decrease in the amount of ZO 1 or occludin . ( 3 ) The amount of claudin 1 present in the cell is decreased with increasing amounts of TNFalpha suggesting that the lack of claudin 1 may cause a relocation of ZO 1 away from the TJ . ( 4 ) This rearrangement may play a role in the increased intestinal permeability seen in CD and other diseases . . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Deprivation of GLN decreased claudin 1 , occludin , and ZO 1 protein expression and caused a disappearance of perijunctional claudin 1 and a reduction of occludin but had no effect on ZO 1 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The expression and localization of the adherens and tight junction proteins E cadherin , occludin , ZO 1 , claudin 1 and claudin 2 were determined by western blotting , RT PCR and immunocytochemistry . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Expression of connexin 43 , an integral component of gap junctions , the tight junction molecules claudin 1 and zonula occludens 1 ( ZO 1 ) , and the catalytic alpha subunit of Na ( + ) K ( + ) ATPase was demonstrated by SDS PAGE and Western blotting . ^^^ Both claudin 1 and ZO 1 immunostaining showed plasma membrane staining , which was present at the stratum granulosum with decreasing intensity through the stratum spinosum to the stratum basale . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The CAMs at TJs , claudin 1 , occludin and JAM 1 , or the PMPs at TJs , ZO 1 and MAGI 1 , were not concentrated there , either . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The purpose of this study was to determine the pattern of expression and prognostic value of four tight junction associated proteins , claudin 1 , claudin 4 , occludin and ZO 1 in a cohort of TNM stage 2 colon cancer using tissue microarray technology . ^^^ In this study , we retrospectively analyzed , resected and otherwise untreated paraffin embedded specimens from 129 consecutive patients with TNM stage 2 colonic carcinomas for claudin 1 , claudin 4 , occludin and ZO 1 protein expression by immunohistochemistry . ^^^ Seventy five , 58 , 56 and 44 % of the tumors exhibited normal to elevated expression levels ( +2 and +3 immunopositivity ) of claudin 1 , claudin 4 , occludin and ZO 1 respectively . ^^^ Low expression levels of claudin 1 and ZO 1 were directly associated with higher tumor grade ( P=0 . 05 and 0 . 03 respectively ) . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The expression and distribution of the TJ associated proteins actin , occludin , claudin 1 , 3 , and 5 , and ZO 1 and 2 were analyzed by Western blot and immunofluorescence microscopy . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
This correlates with a decrease in junctional proteins claudin 1 and E cadherin , in addition to the internalization of the tight junction protein ZO 1 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Treatment with RA had only a slight effect on the mRNA expression of the tight junction associated proteins occludin , ZO 1 , claudin 1 , 3 , and 4 , but enhanced the expression of claudin 2 , which was recently suggested to form a paracellular ion channel . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Localization studies in mucosal biopsies from IBD patients have revealed disappearance of key TJ ( occludin , JAM 1 , ZO 1 , claudin 1 ) and AJ ( E cadherin , beta catenin ) proteins from intercellular junctions . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Tight junction associated proteins ( occludin , ZO 1 , claudin 1 , claudin 4 ) in squamous cell carcinoma and Bowen ' s disease . ^^^ OBJECTIVES : To examine the expression of TJ associated proteins ( occludin , ZO 1 , claudin 1 and claudin 4 ) in normal human epidermis and in malignant disorders of keratinization . ^^^ RESULTS : In normal human epidermis , occludin , ZO 1 and claudin 4 were expressed at the cell cell borders in the granular layer specifically or dominantly , whereas claudin 1 was expressed in the whole epithelium . ^^^ In SCC , occludin , ZO 1 , claudin 1 and claudin 4 were strongly expressed in tumour cells with keratinization such as cancer pearls . ^^^ Claudin 1 was heterogeneously expressed in unkeratinized tumour cells , whereas expression of occludin , ZO 1 and claudin 4 was decreased or absent . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
These include morphological ( light and transmission electron microscopy [ TEM ] ) , protein expression ( immunologically positive for ZO 1 , claudin 1 , and E cadherin ) , and functional evidence . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Marked changes in cytoskeletal F actin distribution / polymerization and a significant reduction in the junctional expression of the tight junctional proteins occludin and claudin 1 but subtle alterations in ZO 1 were observed following protamine elicited paracellular barrier disruption . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
In reverse transcription polymerase chain reaction , mRNAs of tight junction proteins occludin , JAM 1 , ZO 1 , and claudin 1 , 4 , 7 , 8 , 12 , 13 , and 14 were detected in the nasal mucosa . ^^^ Occludin , JAM 1 , and ZO 1 were colocalized in the uppermost layer in the pseudostratified epithelium of the nasal mucosa , whereas claudin 1 , 4 , and 7 were found throughout the epithelium . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Formalin fixed , paraffin embedded sections from 23 primary and seven recurrent or metastatic cases of genetically confirmed ES / PNET were immunostained for claudin 1 and occludin ( tight junction structural proteins ) , zonula occludens 1 ( ZO 1 , tight junction linker protein ) , desmoglein 1 / 2 ( desmosomal adherens protein ) , desmoplakin ( desmosomal structural protein ) and E cadherin ( epithelial adherens junction protein ) , using steam heat induced epitope retrieval and the Dako Envision system . ^^^ Claudin 1 was expressed by 19 of 30 specimens ( 63 % ) , ZO 1 was expressed by 15 of 29 specimens ( 51 % ) , and occludin was expressed by three of 28 specimens ( 11 % ) . ^^^ Our data suggest that many of the previously described cell cell junctions in ES / PNET are poorly formed tight junctions , given the high frequency of claudin 1 and ZO 1 expression . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Madin Darby canine kidney ( MDCK ) cells expressing enhanced green fluorescent protein , enhanced yellow fluorescent protein , or monomeric red fluorescent protein 1 fusion proteins of beta actin , occludin , claudin 1 , ZO 1 , clathrin light chain A 1 , and caveolin 1 were imaged by time lapse multidimensional fluorescence microscopy with simultaneous measurement of transepithelial electrical resistance ( TER ) . ^^^ In contrast , ZO 1 and claudin 1 redistribution occurred well after maximal TER loss . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Immunohistochemistry showed expression of claudin 1 , claudin 3 , and ZO 1 in the intercellular junctions of a subpopulation of tumor cells . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Moderate claudin 1 and ZO 1 membranous staining were present , whereas only focal weak claudin 3 and 4 membranous staining was present in normal gastric epithelium . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Real time RT PCR studies revealed that Z 310 cells possess mRNAs encoding ZO 1 , 2 , and 3 , claudin 1 , 2 , 4 , and 8 , occludin , and connexin 32 . ^^^ Confocal microscopic analyses confirmed the presence of claudin 1 and ZO 1 in Z 310 cells at cell cell contact sites . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The occludin peptide redistributed occludin but did not alter the expression or distribution of ZO 1 , claudin 1 , or claudin 4 . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Morphological studies using confocal fluorescence microscopy showed no perturbation of the normal distribution of ZO 1 , occludin or E cadherin but revealed a redistribution of the tight junction associated protein claudin 1 and the perijunctional actin after incubation with HePC . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
Immunoblotting and immunocytochemistry revealed no changes in the expression and distribution of TJ associated proteins occludin , ZO 1 and claudin 1 in confluent monolayers after hormone addition . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The small GTPase Rab 13 localizes to tight junctions in epithelial cells and regulates the recruitment of claudin 1 and ZO 1 , two proteins required for the assembly of functional tight junctions . ^^^ The inhibition of VASP phosphorylation abolishes the targeting of VASP to cell cell junctions , which in turn leads to a delay in the recruitment of claudin 1 and ZO 1 into tight junctions . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
In contrast , these gap junction blocking agents inhibited the barrier function of tight junctions in cells , determined by measurement of transendothelial electrical resistance and paracellular flux of mannitol and inulin . 18beta GA also significantly reduced the barrier property in rat lung endothelial ( RLE ) cells expressing doxycycline induced claudin 1 , but did not change the interaction between Cx 43 and either claudin 1 or ZO 1 , nor their expression levels or subcellular distribution . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
TJ proteins ZO 1 and occludin are markedly redistributed , morphologically and biochemically , but effects on claudin 1 and claudin 2 are limited . tMLCK inhibition prevents changes in barrier function and tight junction organization induced by tMLCK expression , suggesting that these events both require myosin light chain phosphorylation . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The transmembrane tight junction proteins occludin , claudin 1 and claudin 5 , as well as the cytoplasmic accessory proteins ZO 1 and ZO 2 displayed a continuous distribution at cell boundaries . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
The increased zonulin levels correlated with increased intestinal permeability in vivo and changes in claudin 1 , claudin 2 , and myosin IXB genes expression , while no changes were detected in ZO 1 and occludin genes expression . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
While molecular expression of junctional adhesion molecule ( JAM ) , ZO 2 , ZO 3 , claudin 2 and claudin 3 were unaltered , ZO 1 , occludin , and claudin 4 were all up regulated ( 1 . 6 , 1 . 6 , 2 . 4 fold , respectively ; P < 0 . 001 0 . 01 ) and claudin 1 down regulated ( 2 . 5 fold ; P < 0 . 05 ) by trans 10 , cis 12 CLA , which may underpin its effects on tight junction function and paracellular Ca transport . ^^^
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA
Interacting proteins: O95471 and Q07157	Pubmed	SVM Score :0.0
NA