| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :1.0357027 |
| MED 1 , a novel human methyl CpG binding endonuclease , interacts with DNA mismatch repair protein MLH 1 . 1.0357027^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.80174917 |
| The base excision repair ( BER ) enzyme MED 1 ( also known as MBD 4 ) interacts with the MMR protein MLH 1 . 0.80174917^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| The human protein MED 1 ( also known as MBD 4 ) was previously isolated in a two hybrid screening using the mismatch repair protein MLH 1 as a bait , and shown to have homology to bacterial base excision repair DNA N glycosylases / lyases . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| The human DNA repair protein MED 1 ( also known as MBD 4 ) was isolated as an interactor of the mismatch repair protein MLH 1 in a yeast two hybrid screening . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| All MBDs except MBD 4 , an endonuclease that forms a complex with the DNA mismatch repair protein MLH 1 , form complexes with histone deacetylase . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| MBD 4 encodes a protein that interacts with the mismatch repair protein hMLH 1 . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| MBD 4 was originally identified through its methyl binding domain , but has more recently been characterized as a thymine DNA glycosylase that interacts with the mismatch repair ( MMR ) protein MLH 1 . ^^^ As MLH 1 and other MMR proteins have been functionally linked to apoptosis , we asked whether MBD 4 also plays a role in mediating the apoptotic response within the murine small intestine . ^^^ We next analysed the apoptotic response to 5 FU and temozolomide in doubly mutant Mbd 4 ( / ) , Mlh 1 ( / ) mice but observed no additive decrease . ^^^ The results imply that MBD 4 and MLH 1 lie in the same pathway and therefore that MMR dependent apoptosis is mediated through MBD 4 . ^^^ MBD 4 deficiency also reduced the normal apoptotic response to gamma irradiation , which we show is independent of Mlh 1 status ( at least in the murine small intestine ) , so suggesting that the reliance upon MBD 4 may extend beyond MMR mediated apoptosis . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| No associations were seen between MSI and immunohistochemical expression of hMLH 1 , hMSH 2 , alterations in p 53 or MBD 4 , tumor grade , pathologic stage , or patient survival . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| The thymine DNA glycosylase MBD 4 represses transcription and is associated with methylated p 16 ( INK4a ) and hMLH 1 genes . ^^^ Furthermore , a chromatin immunoprecipitation assay clearly shows that MBD 4 binds to hypermethylated promoters of the p 16 ( INK4a ) and hMLH 1 genes . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We demonstrated that MeCP 2 , MBD 1 and MBD 2 , but not MBD 3 and MBD 4 , specifically bind to methylated hMLH 1 promoters . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| The human protein MED 1 , also known as MBD 4 , was isolated in a yeast two hybrid screening as an interactor of the mismatch repair protein MLH 1 . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| As well as known partners of MLH 1 such as PMS 1 , MLH 3 and MBD 4 , we identified the carboxy terminus of the human c MYC proto oncogene as an interacting sequence . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| The MBD 4 interacting mismatch repair factor MLH 1 was also found in a complex with FADD . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , MSI was not detected in 86MI and it appears to express all the major MMR components hMSH 2 , hMSH 6 , hMLH 1 , hPMS 2 , hMSH 3 , hMLH 3 , MBD 4 ( MED 1 ) and hExo 1 . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| The base excision repair protein MED 1 ( also known as MBD 4 ) , an interactor with the mismatch repair protein MLH 1 , has a central role in the maintenance of genomic stability with dual functions in DNA damage response and repair . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| By western analysis we demonstrated that the HeLa extract contained MED 1 protein , which interacts with MLH 1 and binds to CpG methylated DNA ; supplementation with purified MED 1 protein was without effect . ^^^ |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95243 and P40692 |
Pubmed |
SVM Score :0.0 |
| NA |
|