| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Expression of cellular retinol binding protein and lecithin retinol acyltransferase in developing rat testis . ^^^ Cellular retinol binding protein ( CRBP ) and lecithin retinol acyltransferase ( LRAT ) are two proteins found in Sertoli cells that are known to be involved in vitamin A trafficking . ^^^ Cellular retinol binding protein ( CRBP ) and lecithin retinol acyltransferase ( LRAT ) are two proteins found in Sertoli cells that are known to be involved in vitamin A trafficking . ^^^ |
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| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| However , it has been shown that there is a difference in binding of holo CRBP and apo CRBP to lecithin retinol acyltransferase . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : The accumulation of all trans retinol in neural retina , in the absence of CRBPI and the reduced amount of retinyl esters in the RPE suggest that the binding protein participates in a process that drives diffusion of all trans retinol from photoreceptor cells to RPE , perhaps by delivering vitamin A to lecithin retinol acyltransferase ( LRAT ) for esterification . ^^^ |
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| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| LRAT utilized an endogenous acyl donor and either unbound retinol or retinol complexed with cellular retinol binding protein ( CRBP ) to catalyze the synthesis of retinyl linoleate , retinyl oleate , retinyl palmitate , and retinyl stearate . ^^^ |
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| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| We assessed the effects of excess dietary vitamin A or beta carotene on the cellular retinol binding protein , type 2 [ CRBP ( 2 ) ] level and activities of lecithin : retinol acyltransferase ( LRAT ) and acyl CoA : retinol acyltransferase ( ARAT ) in rat intestine . ^^^ |
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| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Inhibition of lecithin retinol acyltransferase ( LRAT ) with 10 N acetamidodecyl chloromethyl ketone ( AcDCMK ) or cellular retinol binding protein 1 ( CRBP ) diminished the generation of both retinyl esters and 11 cis retinol from all trans retinol . ^^^ |
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| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| We demonstrated that retinol bound to CRBP 3 is an excellent substrate for lecithin retinol acyltransferase , the enzyme responsible for catalyzing retinyl ester formation from retinol . ^^^ Our data indicated that the diminished milk retinyl ester levels arise from impaired utilization of retinol by lecithin retinol acyltransferase in CRBP 3 ( / ) mice . ^^^ |
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| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Esterification of free retinol by both liver and intestinal LRAT resulted in Km values ( 0 . 63 and 0 . 44 microM , respectively ) similar to those obtained for esterification of retinol CRBP ( 0 . 20 and 0 . 78 microM , respectively ) and esterification of retinol CRBP ( 2 ) ( 0 . 24 and 0 . 32 microM , respectively ) . ^^^ Evidence for such interaction was obtained when apo CRBP proved to be a potent competitive inhibitor of LRAT , with a KI ( 0 . 21 microM ) lower than the Km for CRBP retinol ( 0 . 78 microM ) . ^^^ Apo CRBP reacted with 4 mM p ( chloromercuri ) benzenesulfonic acid lost retinol binding ability but retained the ability to inhibit LRAT , confirming that the inhibition could not be explained by a reduction in the concentration of free retinol . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
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| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| The patterns of expression of cellular retinol binding protein ( CRBP ) , cellular retinol binding protein , type two [ CRBP ( 2 ) ] , lecithin : retinol acyltransferase ( LRAT ) , and microsomal retinal reductase were examined for rat small intestine during the perinatal period . ^^^ |
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| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| In animals depleted of retinol , as judged by undetectable liver vitamin A stores and low plasma retinol concentrations , hepatic LRAT activity was almost undetectable , whether assayed with retinol bound to cellular retinol binding protein or solvent dispersed retinol . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Retinol bound to cellular retinol binding protein ( CRBP ) or dispersed in solvent was esterified in a fatty acyl CoA independent , PMSF sensitive reaction , consistent with lecithin : retinol acyltransferase ( LRAT ) activity . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| In the mucosal layer of the small intestine , we found nearly identical gradients of CRBP ( 2 ) , retinal reductase , and LRAT levels down the duodenal ileal axis , suggesting coordinate regulation of these three proteins . ^^^ An even distribution of LRAT activity along the longitudinal axis of the intestinal muscle was consistent with an even distribution of CRBP in that tissue . ^^^ In conjunction with LRAT activity and CRBP , we found endogenous retinyl ester stores in the intestinal muscle layer . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| The activity of LRAT was assayed both by monitoring the esterification of retinol bound to the cellular retinol binding protein ( CRBP ) and of solvent dispersed retinol . ^^^ The slopes obtained with both LRAT assays were similar , yielding target sizes of 52 + / 10 kDa ( n = 10 ) for the LRAT assay with CRBP retinol and 56 + / 7 kDa ( n = 6 ) for the LRAT assay with dispersed retinol . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| To characterize developmental changes in retinol absorptive capability in intestine , we determined LRAT activity and the amount of its retinol donor , cellular retinol binding protein , type two ( CRBP ( 2 ) ) in the duodenum of developing chicks . ^^^ The LRAT activity in duodenal microsomes was very low at 18 and 20 day chick embryo , but exhibited a rapid ( 15 fold ) increase during 48 h around hatching , which occurred in parallel with the abrupt elevation of the content of CRBP ( 2 ) in chick duodenum . ^^^ To examine whether dietary vitamin A affects the developmental change in LRAT activity and CRBP ( 2 ) content , 1 day old chicks were pair fed vitamin A depleted or vitamin A supplemented diet for 14 days . ^^^ The chicks fed vitamin A depleted diet showed significantly reduced LRAT activity and CRBP ( 2 ) in duodenum as early as 3 days after the start of the vitamin A depleted diet . ^^^ These results suggest that duodenal LRAT activity and CRBP ( 2 ) are modulated by dietary vitamin A during the perinatal period . . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| To determine whether 4 HPR , like retinoic acid , is able to induce liver LRAT activity , vitamin A deficient rats having negligible liver LRAT activity were treated with single doses of 4 HPR ( 0 . 02 2 . 5 mg ) and liver homogenates were assayed for LRAT activity using 3H retinol bound to the cellular retinol binding protein , CRBP , as substrate . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| These results suggest that the beta carotene cleavage enzyme is coordinately distributed along the villus crypt axis with retinal reductase and LRAT , the two enzymes which require cellular retinol binding protein , typeII ( CRBPII ) as the donor of the substrate . . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Retinoid content , enzyme activities catalysing the esterification of ROL ( ARAT and LRAT ) and the hydrolysis of RE ( REH ) , as well as retinol binding protein ( CRBP 1 ) expression were determined in the epidermis . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Lecithin : retinol acyltransferase ( LRAT ) , present in microsomes , catalyzes the transfer of the sn 1 fatty acid of phosphatidylcholine to retinol bound to a cellular retinol binding protein . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| These developmental inductions of beta carotene cleavage enzyme and retinal reductase activities in the duodenum coincided with those of cellular retinol binding protein , type 2 ( CRBPII ) and lecithin : retinol acyltransferase ( LRAT ) . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Expressions of hepatic lecithin : retinol acyltransferase ( LRAT ) and cellular retinol binding protein were analyzed with reverse transcription polymerase chain reaction . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Activation of cultured hepatic stellate cells correlated with an enhanced expression of proteins involved in uptake and storage of fatty acids ( FA translocase CD 36 , Acyl CoA synthetase 2 ) and retinol ( cellular retinol binding protein type 1 , CRBP 1 ; lecithin : retinol acyltransferases , LRAT ) . ^^^ The increased expression of CRBP 1 and LRAT during hepatic stellate cells activation , both involved in retinol esterification , was in contrast with the simultaneous depletion of their typical lipid vitamin A ( vitA ) reserves . ^^^ Administration of L 165041 , a PPARbeta specific agonist , further induced the expression of CD 36 , B FABP , CRBP 1 , and LRAT , whereas their expression was inhibited by antisense PPARbeta mRNA . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Levels of atRA and the expression of two potentially retinoic acid ( RA ) controlled proteins critically involved in retinoid storage regulation , lecithin : retinol acyltransferase ( LRAT ) and cellular retinol binding protein 1 ( CRBP 1 ) , were analyzed in liver and kidney . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Lecithin : retinol acyltransferase ( LRAT ) , a microsomal enzyme present in liver and several other retinol metabolizing tissues , esterifies retinol that is associated with a cellular retinol binding protein , CRBP or CRBP 2 . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| RESULTS : CRALBP , IRBP , cellular retinol binding protein ( CRBP ) , 11 cis retinol dehydrogenase ( 11 cis RDH ) , lecithin : retinol acyltransferase ( LRAT ) , and ATP binding cassette receptor ( ABCR ) were detected in human CE tissue by RT PCR . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| An assay of LRAT and ARAT activities along the villus crypt axis of the duodenum by a cryostat sectioning technique revealed that between the day of hatching and 1 d posthatch , an abrupt induction of LRAT activity occurred only in the villus region of the duodenum , where a coordinated induction of cellular retinol binding protein , type 2 ( CRBPII ) , was observed . ^^^ These observations in the developmental changes and distribution of LRAT and ARAT activities suggest that LRAT activity but not ARAT activity is closely related to the induction of CRBPII in the duodenum of developing chicks . . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| The objective of this study was to investigate whether age and / or VA status are factors for the hepatic expression of cellular retinol binding protein ( CRBP ) , the esterification of retinol by lecithin : retinol acyltransferase ( LRAT ) and the accumulation of VA and lipids in liver . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| Unesterified retinol taken up by the enterocyte is complexed with cellular retinol binding protein type 2 and the complex serves as a substrate for reesterification of the retinol by the enzyme lecithin : retinol acyltransferase ( LRAT ) . ^^^ |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O95237 and P09455 |
Pubmed |
SVM Score :0.0 |
| NA |
|