| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| Mitochondrial fusion in higher eukaryotes requires at least two essential GTPases , Mitofusin 1 and Mitofusin 2 ( Mfn 2 ) . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| The mitofusins ( Mfn 1 and Mfn 2 ) are homologs of the Drosophila protein fuzzy onion ( Fzo ) that associate with mitochondria and alter mitochondrial morphology when expressed by transient transfection in tissue culture cells . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| Two human Fzo homologs , mitofusins Mfn 1 and Mfn 2 , are shown by RT PCR and western blot to be ubiquitous mitochondrial proteins . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| Mitofusins Mfn 1 and Mfn 2 coordinately regulate mitochondrial fusion and are essential for embryonic development . ^^^ The mitofusins , Mfn 1 and Mfn 2 , have been shown to affect mitochondrial morphology when overexpressed . ^^^ We find that mice deficient in either Mfn 1 or Mfn 2 die in midgestation . ^^^ However , whereas Mfn 2 mutant embryos have a specific and severe disruption of the placental trophoblast giant cell layer , Mfn 1 deficient giant cells are normal . ^^^ Embryonic fibroblasts lacking Mfn 1 or Mfn 2 display distinct types of fragmented mitochondria , a phenotype we determine to be due to a severe reduction in mitochondrial fusion . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| The nuclear encoded mitochondrial proteins Mfn 1 and Mfn 2 are human homologues of the only known protein mediators of mitochondrial fusion , the Drosophila Fzo GTPase and Saccharomyces cerevisiae yFzo1p . ^^^ Although the Mfn 1 and Mfn 2 genes were broadly expressed , the two genes showed different levels of mRNA expression in different tissues . ^^^ Two Mfn 1 transcripts were detected at similar levels in a variety of human tissues and were dramatically elevated in heart , while Mfn 2 mRNA was abundantly expressed in heart and muscle tissue but present only at low levels in many other tissues . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| We characterized two rat mitochondrial outer membrane proteins , Mfn 1 and Mfn 2 , with distinct tissue expressions , that are homologous to Drosophila Fzo , a GTPase involved in mitochondrial fusion . ^^^ The Mfn 1 induced tubular networks were suppressed by co expression with Mfn 2 . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| A genetic analysis proved that OPA 1 was unable to tubulate and fuse mitochondria lacking the outer membrane mitofusin 1 but not mitofusin 2 . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| The mitochondrial fusion factors mitofusins 1 and 2 ( Mfn 1 and Mfn 2 ) and the fission factor dynamin related protein 1 ( Drp 1 ) were found to be highly expressed in the pubertal and adult rat testis by Northern blot analysis . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| These experiments revealed that Mfn 1 harboring mitochondria were efficiently tethered in a GTP dependent manner , whereas Mfn 2 harboring mitochondria were tethered with only low efficiency . ^^^ Mfn 1 exhibited higher activity for this reaction than Mfn 2 . ^^^ Purified recombinant Mfn 1 exhibited approximately eightfold higher GTPase activity than Mfn 2 . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| Here , we show that cells with targeted null mutations in Mfn 1 or Mfn 2 retained low levels of mitochondrial fusion and escaped major cellular dysfunction . ^^^ In contrast , cells lacking both Mfn 1 and Mfn 2 completely lacked mitochondrial fusion and showed severe cellular defects , including poor cell growth , widespread heterogeneity of mitochondrial membrane potential , and decreased cellular respiration . ^^^ |
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| Interacting proteins: Q8IWA4 and O95140 |
Pubmed |
SVM Score :0.0 |
| To address if Mfn proteins are implicated in these exercise induced responses , we measured Mfn 1 and Mfn 2 mRNA levels , pre , post , 2 and 24 h post exercise . ^^^ We show that Mfn 1 , Mfn 2 , NRF 2 and COX 4 mRNA were increased 24 h post exercise , while PGC 1alpha and ERRalpha mRNA increased 2 h post exercise . ^^^ |
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