| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| The carboxy terminal glycine residue of Apg 12 , a 186 amino acid protein , is conjugated to a lysine at residue 149 of Apg 5 , a 294 amino acid protein . ^^^ Furthermore , Apg 5 and Apg 12 have mammalian homologues , suggesting that this new modification system is conserved from yeast to mammalian cells . . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| The covalent modification of Apg 5 with Apg 12 is not required for its membrane targeting , but is essential for involvement of Apg 5 in elongation of the isolation membranes . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| This process requires a ubiquitin like protein conjugation system , in which Apg 12 is covalently bound to Apg 5 . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| Apg 12 is finally conjugated to Apg 5 via an isopeptide bond . ^^^ The in vivo interaction of mApg 12 with mApg 10 in HeLa cells suggests that mApg 10 is an Apg 12 conjugating enzyme , likely serving as an Apg 5 recognition molecule in the Apg 12 system . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| We also found that membrane targeting of Apg16L requires Apg 5 but not Apg 12 . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| Apg 12 conjugation of Apg 5 is required for elongation of the isolation membrane to form a complete spherical autophagosome . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| In the yeast , Saccharomyces cerevisiae , two ubiquitin like modifications , Apg 12 conjugation with Apg 5 and Apg 8 lipidation with phosphatidylethanolamine , are essential for autophagy and the cytoplasm to vacuole transport of aminopeptidase 1 ( Cvt pathway ) . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| Mammalian cells were observed to die under conditions in which nutrients were depleted and , simultaneously , macroautophagy was inhibited either genetically ( by a small interfering RNA targeting Atg 5 , Atg6 / Beclin 1 1 , Atg 10 , or Atg 12 ) or pharmacologically ( by 3 methyladenine , hydroxychloroquine , bafilomycin A 1 , or monensin ) . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| In yeast , this encapsulation employs a set of autophagy ( ATG ) proteins that direct the conjugation of two ubiquitin like protein tags , ATG 8 and ATG 12 , to phosphatidylethanolamine and the ATG 5 protein , respectively . ^^^ By reverse genetic analyses of the single Arabidopsis gene encoding ATG 5 , we show here that the subpathway that forms the ATG 12 ATG5 conjugate also has an essential role in plant nutrient recycling . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| Atg 5 is a novel 34 kDa protein that is covalently modified by Atg 12 , a ubiquitin like modifier , and forms a complex with Atg 16 . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| Mutagenic analyses suggested that this conjugation was formed via an isopeptide bond between the C terminal glycine of hApg 12 and Lys 130 of hApg 5 . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| Expressions of mannose 6 phosphate receptor ( M6PR ) , clathrin and hApg 5 and hApg 12 were analyzed in muscle biopsy specimens from patients with s IBM , amyotrophic lateral sclerosis ( ALS ) or peripheral neuropathy and in normal human muscle specimens by means of immunohistochemistry and reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ In s IBM muscles in particular , clathrin and M6PR were often observed inside rimmed vacuoles and in the sarcoplasm of vacuolated or non vacuolated fibers . mRNA levels of hApg 5 and hApg 12 , which are involved in autophagic vacuole formation , as well as of M6PR and clathrin were significantly increased in s IBM muscles in comparison to levels in normal and ALS / peripheral neuropathy muscles . ^^^ |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O94817 and Q9H1Y0 |
Pubmed |
SVM Score :0.0 |
| NA |
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