Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.82386191
Mixed complexes of ClpA , ClpX , and ClpP with the two ATPases bound simultaneously to opposite faces of a single ClpP molecule were seen by electron microscopy . 0.82386191^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.99265448
Recombinant ClpX can also interact with its putative partner protease subunit ClpP in overexpression experiments in 293T cells . 0.99265448^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.98228638
A simple model explains the observed relationships between ATP binding , ATP hydrolysis and functional interactions between ClpX , protein substrates and ClpP . . 0.98228638^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.71099775
ClpP associates with the hexameric ATPases ClpX and ClpA , which can unfold and translocate substrate proteins through the ClpP axial pores into the protease lumen for degradation . 0.71099775^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.7933008
ClpP associates with ClpX and ClpA ATPases that unfold and translocate substrates into the protease catalytic chamber through axial pores located at both ends of the ClpP cylinder . 0.7933008^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Previously , ClpX has been shown to function with the ClpP peptidase in protein turnover . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The second 46 , 000 Da component was identified as ClpX ( LopC ) , coded by a gene located in the same operon , but promoter distal to that coding for ClpP ( Gottesman , S . , Clark , W . ^^^ These results suggest that ClpX protein directs ClpP protease to specific substrates . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The amino terminal sequence of this protein corresponds to the translated amino terminal sequence of a gene that we have named clpX . clpX encodes a protein with M ( r ) 46 , 300 , similar to that observed for the protein purified by Wojtkowiak et al . clpX is an operon with clpP ; both genes are cotranscribed in a single heat inducible 2200 base mRNA , with clpP the promoter proximal gene . ^^^ Mutations in either clpX or clpP abolish degradation of the highly unstable lambda O protein in vivo . clpX mutants are not defective in degradation of previously identified ClpA / ClpP substrates such as a ClpA beta galactosidase fusion protein . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Western immunoblot analysis showed that in mutants lacking the protease ClpP or its cognate ATPase containing subunit ClpX , sigma s levels of exponential phase cells increased to those of stationary phase wild type cells . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The ClpX heat shock protein of Escherichia coli , the ATP dependent substrate specificity component of the ClpP ClpX protease , is a novel molecular chaperone . ^^^ Previously , we identified the ClpX heat shock protein of E . coli because it enables the ClpP catalytic protease to degrade the bacteriophage lambda O replication protein . ^^^ We propose that the ClpX protein is a bona fide chaperone , whose biological role includes the maintenance of certain polypeptides in a form competent for proteolysis by the ClpP protease . ^^^ Furthermore , our results suggest that the ClpX protein also performs typical chaperone protein functions independent of ClpP . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
CodX also shares homology with a family of ATPases , including ClpX , a regulatory subunit of the E . coli ClpP protease . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Recently , a new potential ClpP associated ATPase , ClpX , has been described . ^^^ Moreover , ClpX ( but not ClpP ) was found to be required for normal Mu replication . ^^^ Thus ClpX has activities that do not require its association with ClpP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpX , an alternative ATP binding subunit for protease Ti ( also called Clp ) , has been shown to support the ATP dependent hydrolysis of lambda O protein by ClpP . clpX has also been reported to be in an operon with clpP , and therefore both are co transcribed in a single mRNA using the promoter proximal to clpP . ^^^ Here , we show that clpX can be expressed independently from clpP using its own promoter . ^^^ Thus , it appears that clpX can be expressed alone and / or co expressed with clpP in cells depending on physiological conditions . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Stress induction of the Bacillus subtilis clpP gene encoding a homologue of the proteolytic component of the Clp protease and the involvement of ClpP and ClpX in stress tolerance . ^^^ Mutants lacking either the proteolytic component ClpP or the regulatory ATPase component ClpX were phenotypically distinct from the wild type . ^^^ Comparison of two dimensional protein gels from wild type cells with those from clpP and clpX mutant cells revealed several changes in the protein pattern . ^^^ Several proteins , such as GroEL , PpiB , PykA , SucD , YhfP , YqkF , YugJ and YvyD , which were found preferentially in higher amounts in both clpP and clpX mutants , might be potential substrates for the ClpXP protease . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The apparent half life of the heterodimeric UmuD / D ' complex is greatly increased in the clpX : : Kan and clpP : : Kan strains and these strains are correspondingly rendered virtually UV non mutable . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
However , in contrast to Ec , clpX and clpP of Bs are located at different loci on the chromosome and are transcribed as monocistronic genes . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
HslU / ClpY is 50 % identical to the ClpX protein of E . coli , which is known to present large polypeptides to its partner , the ATP independent proteolytic enzyme ClpP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Similar to E . coli , a clpX homologue was identified downstream of clpP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Although ClpXP can degrade MuA , the presence of both ClpP and ClpX in the reconstituted transposition system did not destroy MuA essential for initiation of DNA replication by specific host replication enzymes . ^^^ Levels of ClpXP needed to overcome inhibition by the repressor did not prevent MuA from promoting strand transfer , and ClpP stimulated alteration of the transpososome by ClpX . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Degradation in vitro of SsrA tagged substrates was reproduced with purified components and required a substrate with a wild type SsrA tail , the presence of both ClpP and either ClpA or ClpX , and ATP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpX and ClpP are essential for the efficient acquisition of genes specifying type IA and IB restriction systems . ^^^ We show that ClpX and ClpP , the components of ClpXP protease , are necessary for the efficient transmission of the genes encoding EcoKI and EcoAI , representatives of two families of type 1 R M systems , thus implicating ClpXP in the modulation of restriction activity . ^^^ Loss of ClpX imposed a bigger barrier than loss of ClpP , consistent with a dual role for ClpX , possibly as a chaperone and as a component of the ClpXP protease . ^^^ Transmission of genes specifying EcoKI was more dependent on ClpX and ClpP than transmission of the genes for EcoAI . ^^^ In the absence of either ClpX or ClpP transfer of the EcoKI genes by P 1 mediated transduction was impaired , transfer of the EcoAI genes was not . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Comparison of the deduced amino acid sequences of the C . crescentus ClpP and ClpX proteins with those of their homologues from several gram positive and gram negative bacteria revealed stronger conservation for the ATPase regulatory subunit ( ClpX ) than for the peptidase subunit ( ClpP ) . ^^^ The clpP and clpX genes are separated on the C . crescentus chromosome by an open reading frame pointing in the opposite direction from the clp genes , and transcription of clpP and clpX was found to be uncoupled . clpP is transcribed as a monocistronic unit with a promoter ( PP 1 ) located immediately upstream of the 5 ' end of the gene and a terminator structure following its 3 ' end . ^^^ At least three promoters for clpX ( PX 1 , PX 2 , and PX 3 ) were mapped in the clpP clpX intergenic region . ^^^ In addition , the clpP and clpX promoters showed different activity patterns during the cell cycle . ^^^ Determination of the numbers of ClpP and ClpX molecules per cell suggested that ClpX is the limiting component compared with ClpP . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpXP is composed of two types of polypeptides , the ClpX ATPase and the ClpP peptidase . ^^^ Analysis of mutants lacking ClpX or ClpP revealed that both proteins are required in vivo for the cell cycle dependent degradation of the regulatory protein CtrA . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The ClpA chaperone , but not ClpX , is required for ClpP dependent HemA turnover . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
In Escherichia coli , ClpP is the proteolytic component of a large complex also containing either the ClpA or the ClpX ATPases . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Recognition , targeting , and hydrolysis of the lambda O replication protein by the ClpP / ClpX protease . ^^^ It has previously been established that sequences at the C termini of polypeptide substrates are critical for efficient hydrolysis by the ClpP / ClpX ATP dependent protease . ^^^ We report for the bacteriophage lambda O replication protein , however , that N terminal sequences play the most critical role in facilitating proteolysis by ClpP / ClpX . ^^^ However , ClpX can not efficiently promote the ATP dependent binding of this truncated O polypeptide to ClpP , the catalytic subunit of the ClpP / ClpX protease . ^^^ Based on our results with lambda O protein , we suggest that two distinct structural elements may be required in substrate polypeptides to enable efficient hydrolysis by the ClpP / ClpX protease : ( 1 ) a ClpX binding site , which may be located remotely from substrate termini , and ( 2 ) a proper N or C terminal sequence , whose exposure on the substrate surface may be induced by the binding of ClpX . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The genes of Streptomyces coelicolor A 3 ( 2 ) encoding catalytic subunits ( ClpP ) and regulatory subunits ( ClpX and ClpC ) of the ATP dependent protease family Clp were cloned , mapped and characterized . ^^^ S . coelicolor contains at least two clpP genes , clpP 1 and clpP 2 , located in tandem upstream from the clpX gene , and at least two unlinked clpC genes . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
We now provide evidence that MviA ( RssB ) directly interacts both with sigmaS and ClpX in vivo , presumably enabling presentation of sigmaS to the ClpP protease . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
These included 20 mutants defective in mviA , the ortholog of Escherichia coli rssB / sprE , and 13 mutants defective in either clpP or clpX which encode the protease active on RpoS . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The molecular characterisation of three stress genes hsp 18 , clpX , trxA encoding for a small heat shock protein , an ATPase regulation component of ClpP protease and a thioredoxin , respectively , allow us to suggest the existence in O . oeni of multiple regulation mechanisms as is the case in Bacillus subtilis . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The presence of the heat stress response related ATPases ClpC and ClpX or the peptidase ClpP in the cell is crucial for tolerance of many forms of stress in Bacillus subtilis . ^^^ Assays for detection of defects in protein degradation suggest that ClpC , ClpP , and ClpX participate directly in overall proteolysis of misfolded proteins . ^^^ Turnover rates for abnormal puromycyl peptides are significantly decreased in clpC , clpP , and clpX mutant cells . ^^^ By using immunogold labeling with antibodies raised against ClpC , ClpP , and ClpX , the Clp proteins were localized in these aggregates , showing that the Clp proteins act at this level in vivo . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpXP is a protein machine composed of the ClpX ATPase , a member of the Clp / Hsp100 family of remodeling enzymes , and the ClpP peptidase . ^^^ ClpX translocates this denatured protein into the proteolytic chamber of ClpP and , when proteolysis is blocked , also catalyzes release of denatured GFP ssrA from ClpP in a reaction that requires ATP and additional substrate . ^^^ Kinetic experiments reveal that multiple reaction steps require collaboration between ClpX and ClpP and that denaturation is the rate determining step in degradation . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpX and ClpA are molecular chaperones that interact with specific proteins and , together with ClpP , activate their ATP dependent degradation . ^^^ As the protein is unfolded by ClpX , additional motifs are exposed that facilitate its retention and favor its translocation to ClpP for degradation . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Mutations conferring amino acid residue substitutions in the carboxy terminal domain of RNA polymerase alpha can suppress clpX and clpP with respect to developmentally regulated transcription in Bacillus subtilis . ^^^ The Bacillus subtilis clpX and clpP genes are the sites of pleiotropic mutations that adversely affect growth on a variety of media and impair developmental processes such as sporulation and competence development . ^^^ Both ClpX and ClpP are required for the activation of sigmaA dependent transcription of the srf operon that encodes surfactin synthetase and the regulatory peptide ComS , required for the development of genetic competence . ^^^ These findings provide further support for the hypothesis that ClpX and ClpP might be intimately associated with transcription initiation in B . subtilis . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpP / ClpX mediated degradation of the bacteriophage lambda O protein and regulation of lambda phage and lambda plasmid replication . ^^^ This protein , although protected from proteases by other elements of the replication complex , in a free form is rapidly degraded in the host , Escherichia coli , by the ClpP / ClpX protease . ^^^ However , lambda plasmid copy number in bacteria devoid of the ClpP / ClpX protease was not dependent on the bacterial growth rate and in all minimal media tested was comparable to that observed in wildtype cells growing in a rich medium . ^^^ Contrary to lambda plasmid replication , the efficiency of lytic growth of bacteriophage lambda was found to be dependent on the host growth rate in both wild type bacteria and clpP and clpX mutants . ^^^ These results indicate that the O protein ( whose level in E . coli cells depends on the activity of ClpP / ClpX protease ) is a major limiting factor in the regulation of lambda plasmid replication at low bacterial growth rates . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Transcriptional analysis of a clpP ' bgaB fusion and / or Western blotting experiments using antibodies directed against the CtsR protein indicate that ClpP and ClpX are involved in CtsR degradation at 37 degrees C . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Expression of different size transcripts from the clpP clpX operon of Escherichia coli during carbon deprivation . ^^^ Transcription of the clpP clpX operon of Escherichia coli leads to the production of two different sizes of transcripts . ^^^ The longer transcript consists of the entire clpP clpX operon , whereas the shorter transcript contains the entire clpP gene but none of the clpX coding sequence . ^^^ Primer extension experiments suggest that there is increased usage of the sigma ( 32 ) dependent promoter of the clpP clpX operon within 15 min after the start of carbon starvation . ^^^ Expression of the clpP clpX operon from the promoters upstream of the clpP gene decreases to a very low level by 20 min after the onset of carbon starvation . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
When ATP was added to proteolytically inactive ClpXP lambda O complexes , the extra density transferred to the center of ClpP and remained inside ClpP after separation from ClpX . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Here , we report the identification and characterization of cicA , a gene located between the clpX and clpP genes on the Caulobacter chromosome . cicA is a novel morphogene in C . crescentus and , like clpX and clpP , is essential for growth . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
In Escherichia coli , for instance , the ClpXP protease is assembled from the ClpX ATPase and the ClpP peptidase . ^^^ Here , we have used multiple sequence alignments to identify a tripeptide ' IGF ' in E . coli ClpX that is essential for ClpP recognition . ^^^ Mapping of the IGF loop onto a homolog of known structure suggests a model for ClpX ClpP docking . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
To investigate the contribution of the ClpXP protease to the virulence of serovar Typhimurium we initially cloned the clpP and clpX operon from the pathogenic strain serovar Typhimurium chi 3306 and then created insertional mutations in the clpP and / or clpX gene . ^^^ The Delta clpP and Delta clpX mutants were used to inoculate BALB / c mice by either the intraperitoneal or the oral route and found to be limited in their ability to colonize organs of the lymphatic system and to cause systemic disease in the host . ^^^ An oxygen dependent killing assay using hydrogen peroxide and paraquat ( a superoxide anion generator ) and a serum killing assay using murine serum demonstrated that all of the serovar Typhimurium Delta clpP and Delta clpX mutants were as resistant to these killing mechanisms as the wild type strain . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Finally , the association of the protease ClpP and its ATPase subunits ClpC and ClpX with the PorA inclusion bodies was demonstrated by means of the immunogold labelling technique . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Escherichia coli ClpA and ClpX are ATP dependent protein unfoldases that each interact with the protease , ClpP , to promote specific protein degradation . ^^^ We have used limited proteolysis and deletion analysis to probe the conformations of ClpA and ClpX and their interactions with ClpP and substrates . ^^^ Binding of ClpP blocked this cleavage , and prior cleavage at this site rendered both ClpA and ClpX defective in binding and activating ClpP , suggesting that this site is involved in interactions with ClpP . ^^^ The N terminal domain of ClpX dissociated upon cleavage , and the remaining ClpXDeltaN remained as a hexamer , associated with ClpP , and expressed ATPase , chaperone , and proteolytic activity . ^^^ We propose that the N terminal domains of ClpX and ClpA lie on the outside ring surface of the holoenzyme complexes where they contribute to substrate binding or perform a gating function affecting substrate access to other binding sites and that a loop on the opposite face of the ATPase rings stabilizes interactions with ClpP and is involved in promoting ClpP proteolytic activity . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
We show that the single ATP binding site of ClpX is required for a variety of tasks , namely , the stabilization of the ClpXZn ( 2 ) oligomeric structure , binding to ClpP , and the ClpXP dependent proteolysis of the lambdaO replication protein . ^^^ ClpX , free of Zn ( 2 ) , can not oligomerize , bind to ClpP , or participate in ClpXP dependent proteolysis . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Only disruption of the clpX or clpP gene resulted in stabilization of the tagged substrates . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
In addition to ClpX , a ClpP protein located in mitochondria was also identified from the numerous ClpP isomers in Arabidopsis . ^^^ In Arabidopsis , transcripts for both clpX and clpP 2 genes were detected in various tissues and under different growth conditions , with no significant variation in mRNA level ( i . e . 2 fold ) for each gene between samples . ^^^ Using beta casein as a substrate , plant mitochondria were found to possess an ATP stimulated , serine type proteolytic activity that could be strongly inhibited by antibodies specific for ClpX or ClpP 2 , suggesting an active ClpXP protease . ^^^ The recent discovery of homologous mitochondrial ClpX and ClpP proteins in mammals suggests that this type of protease may be common to multicellular eukaryotes . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
HrcA remained stable only in clpX or clpP knockouts , which suggests that this ATP dependent protease is primarily responsible for the degradation of SsrA tagged proteins in B . subtilis . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Rapid degradation of bacteriophage lambda O protein by ClpP / ClpX protease influences the lysis versus lysogenization decision of the phage under certain growth conditions of the host cells . ^^^ The initiator of bacteriophage lambda DNA replication , the O protein , is rapidly degraded in Escherichia coli by the ClpP / ClpX protease encoded by the host . ^^^ Although the biochemical mechanism of this degradation has been investigated intensively , a physiological role for this process remained unknown since little effect of dysfunction of clpP and clpX genes on the lytic development of the phage was observed . ^^^ Here we demonstrate that activities of clpP and clpX genes influence the lysis versus lysogenization decision of bacteriophage lambda under certain growth conditions of the host cells . ^^^ This decision is influenced specifically by ClpP / ClpX mediated O degradation and resultant inhibition of early lambda DNA replication because mutations in clpP and clpX genes have little effect on stability of other lambda proteins involved in the regulation of the phage developmental switch . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Loss of function mutations in yjbD result in ClpX and ClpP independent competence development of Bacillus subtilis . ^^^ Mutations in clpP and clpX have pleiotropic effects on growth and developmentally regulated gene expression in Bacillus subtilis . ^^^ ClpP and ClpX are needed for expression of comK , encoding the competence transcription factor required for the expression of genes within the competence regulon . ^^^ ClpP and ClpX independent comK expression rendered by inactivation of yjbD was still medium dependent and required ComS . ^^^ Consequently , the yjbD mutation bypasses the defect in competence development conferred by clpP and clpX . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The proteolytic activity is rendered by the ClpP component , while the substrate specificity is determined by the ClpX component that has ATPase activity . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Human mitochondrial ClpP ( hClpP ) and ClpX ( hClpX ) were separately cloned , and the expressed proteins were purified . ^^^ Our results establish that human ClpX and ClpP constitute a bone fide ATP dependent protease and confirm that substrate selection , which differs between human and E . coli ClpX , is dependent solely on the Clp ATPase . ^^^ Our data also indicate that human ClpP has conserved sites required for interaction with eClpX but not eClpA , implying that the modes of interaction with ClpP may not be identical for ClpA and ClpX . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
We constructed and characterized the phenotypes of clpP , clpC , and clpX deletion replacement mutants , which lack the ClpP protease subunit or the putative ClpC or ClpX ATPase specificity factor . ^^^ Besides clpP , transcription of clpC , clpE , and clpL , but not clpX or ftsH , was induced by heat shock or entry into late exponential growth phase . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Proteolytic subunit ClpP , together with regulatory ATPase subunit ClpC or ClpX , is required for the normal response to stress , for development of genetic competence , and for sporulation . ^^^ The spx ( formally yjbD ) gene was previously identified as a site of mutations that suppress defects in competence conferred by clpP and clpX . ^^^ Western blot analysis showed that Spx accumulated in clpX mutant to the same level as that observed in the clpP mutant . ^^^ To exclude a possible effect of clpX and clpP on spx transcription , the spx gene was placed under the control of the IPTG ( isopropyl beta D thiogalactopyranoside ) inducible Pspac promoter . ^^^ In this strain , Spx accumulated when ClpX or ClpP was absent , suggesting that ClpX and ClpP are required for degradation of Spx . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Unlike most bacterial Clp proteins , the Synechococcus ClpP 2 , ClpP 3 , ClpR and ClpX proteins were not highly inducible by high temperatures , or by other stresses such as cold , high light or oxidation , although slower gradual rises occurred for all four proteins during high light , and for ClpP 3 , ClpR and ClpX at low temperature . ^^^ Transcriptional and translational studies further showed differences in the expression and regulation between the clpP clpR clpX genes . ^^^ Similar small increases in ClpP 3 , ClpR and ClpX proteins also occurred in DeltaclpPII . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Both wild type E . coli MC 4100 and lon mutants grew in the presence of 10 % SDS , whereas isogenic clpP and clpB single mutants could not grow above 0 . 5 % SDS and clpA and clpX single mutants could not grow above 5 . 0 % SDS . ^^^ Bacteriol . 173 : 229 233 , 1991 ) are tentatively identified as ClpP , ClpX , and ClpB . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
In ClpXP protease complexes , hexameric rings of the ATP dependent ClpX chaperone stack on one or both faces of the double heptameric rings of ClpP . ^^^ Proteins with N or C terminal recognition motifs bound to complexes at the distal surface of ClpX and , upon addition of ATP , were translocated to ClpP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Homologues of the clpB , clpC , clpE , clpL , clpX , and clpP genes were identified in the S . mutans genome . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The protein was stabilized by the presence of the chaperones ClpA and ClpX and degraded by their cognate protease ClpP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
To elucidate the involvement of proteolysis in the regulation of stationary phase adaptation , the clpA , clpX , and clpP protease mutants of Escherichia coli were subjected to proteome analysis during growth and during carbon starvation . ^^^ For most of the growth phase regulated proteins detected on our gels , the clpA , clpX , or clpP mutant failed to mount the growth phase regulation found in the wild type . ^^^ From our data we conclude that the levels of most major growth phase regulated proteins in E . coli are at some point controlled by the activity of at least one of the ClpP , ClpA , and ClpX proteins . ^^^ Cultures of the strains lacking functional ClpP or ClpX also displayed a more rapid loss of viability during extended stationary phase than the wild type . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Lethality fixation did progress when protein synthesis was restricted and the cells were incubated in the presence of puromycin or were either clpP or clpX defective . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
In this study , we examined the role of the ATPase subunit ClpX , a possible partner of the products of the clpP 1 operon . ^^^ Thus , the clpX phenotype differs from the clpP 1 phenotype , indicating that these two components have only partially overlapping roles . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Essentiality of clpX , but not clpP , clpL , clpC , or clpE , in Streptococcus pneumoniae R 6 . ^^^ We show by using a regulated promoter that clpX of Streptococcus pneumoniae R 6 is essential , whereas clpP , clpL , clpC , and clpE can be disrupted . ^^^ Essentiality of clpX , but not clpP , has not been reported previously . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpX requires ATP to unfold protein substrates and translocate them into the proteolytic chamber of ClpP for degradation . ^^^ ClpX hydrolyzed ATPgammaS to ADP and thiophosphate at a rate ( 6 / min ) significantly slower than ATP hydrolysis ( 140 / min ) , but the hydrolysis of both nucleotides was increased by ssrA tagged substrates and decreased by ClpP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
In more detail , the use of specific antibodies revealed induction of the HSPs DnaK , DnaJ , GroEL , ClpC , ClpP and ClpX of B . weihenstephanensis . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Removal of ZBD from the ClpX sequence renders the ATPase activity of ClpX largely insensitive to the presence of ClpP , substrates , or the SspB cofactor . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
This turnover is dependent on the clpP and clpX genes . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
The crystal structure of ClpX reveals that a conserved tripeptide ( LGF ) is located on the tip of ClpP binding loop extending from the N terminal subdomain . ^^^ A hexameric model of ClpX suggests that six tripeptides make hydrophobic contacts with the hydrophobic clefts of the ClpP heptmer asymmetrically . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpX ( 423 amino acids ) , a member of the Clp / Hsp100 family of molecular chaperones and the protease , ClpP , comprise a multimeric complex supporting targeted protein degradation in Escherichia coli . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Coimmunoprecipitation experiments revealed the predicted specific ClpX ClpP , ClpC ClpP , and ClpE ClpP interactions . ^^^ ClpE and ClpX are rapidly degraded in wild type cells during permanent heat stress but remained almost stable in a clpP mutant , suggesting ClpP dependent degradation . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpX binds substrates bearing specific classes of peptide signals , denatures these proteins , and translocates them through a central pore into ClpP for degradation . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Here , we explore the role of protein stability in ClpX denaturation and subsequent ClpP degradation of model substrates bearing ssrA degradation tags at different positions . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Alternative roles of ClpX and ClpP in Staphylococcus aureus stress tolerance and virulence . ^^^ Presently , we have constructed deletion mutants removing either ClpX or the proteolytic subunit , ClpP , in S . aureus 8325 4 in order to examine a putative link between stress tolerance and virulence . ^^^ When exposed to stress , we found that , although clpP mutant cells were sensitive to conditions generating misfolded proteins , the absence of ClpX improved survival . ^^^ In the presence of oxidative stress or at low temperature , both ClpP and ClpX were important for growth . ^^^ Interestingly , the absence of ClpX or ClpP reduced both transcription of the agr effector molecule , RNA 3 , and the activity of the autoinducing peptide ( AIP ) . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
In E . coli cells with mutant clpA , clpP , of clpX showed a substantially lower luciferase refolding after heat shock . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Interestingly , biofilm formation was reduced in the absence of ClpX or ClpC whereas it was enhanced in the absence of ClpP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpA and ClpX bind substrate proteins through specific recognition signals , catalyze ATP dependent protein unfolding of the substrate , and when in complexes with ClpP translocate the unfolded polypeptide into the cavity of the ClpP peptidase for degradation . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
We suggest that either a novel chaperone presents DnaA to ClpP or that ClpX is used with exceptional efficiency so that when ClpX activity is limiting for CtrA degradation it is not limiting for DnaA degradation . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Strong suppression of the conditional Rex [ Ts ] phenotype was imparted by ssrA and clpP ( polar for clpX ) null mutations , suggesting that RexA or RexB proteins made under conditions of polarity are subject to 10Sa RNA tagging and ClpXP degradation . . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
In a search for additional regulatory genes , we identified a DNA fragment containing clpX and clpP that has a positive regulatory effect on LEE expression in EHEC O 157 . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Genetic data indicate that ClpX but not ClpP inhibits FtsZ ring formation in vivo . ^^^ In vitro , ClpX inhibits FtsZ assembly in a ClpP independent manner through a mechanism that does not require ATP hydrolysis . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpA and ClpX ATPases bind simultaneously to opposite ends of ClpP peptidase to form active hybrid complexes . ^^^ The Escherichia coli ATP dependent ClpAP and ClpXP proteases are composed of a single proteolytic component , ClpP , complexed with either of the two related chaperones , ClpA or ClpX . ^^^ In vitro in the presence of ATP or ATPgammaS , ClpA and ClpX form homomeric rings of six subunits , which bind to one or both ends of the double heptameric rings of ClpP . ^^^ We have observed that , when equimolar amounts of ClpA and ClpX hexamers are added to ClpP in vitro in the presence of ATP or ATPgammaS , hybrid complexes in which ClpX and ClpA are bound to opposite ends of the same ClpP are readily formed . ^^^ The distribution of homomeric and heteromeric complexes was consistent with random binding of ClpA and ClpX to the ends of ClpP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Four Clp ATPases have been identified in Streptomyces coelicolor ( ClpX and three ClpC proteins ) which are potential partners of ClpP 1 ClpP2 . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Human mitochondrial ClpP is a stable heptamer that assembles into a tetradecamer in the presence of ClpX . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Levels of ClpA and ClpP increased about threefold during this transition , whereas ClpX , ClpS and SspB levels remained nearly constant . ^^^ Surprisingly , ClpX dependent ClpP independent degradation of GFP ssrA was also observed . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
By covalently linking active and inactive subunits of the ATPase ClpX to form hexamers , here we show that diverse geometric arrangements can support the enzymatic unfolding of protein substrates and translocation of the denatured polypeptide into the ClpP peptidase for degradation . ^^^ This mechanism would allow any ClpX subunit in contact with a translocating polypeptide to hydrolyse ATP to drive substrate spooling into ClpP , and would prevent stalling if one subunit failed to bind or hydrolyse ATP . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Global virulence regulation in Staphylococcus aureus : pinpointing the roles of ClpP and ClpX in the sar / agr regulatory network . ^^^ Recently , we revealed a new layer of regulation by showing that mutants lacking the ClpXP protease produce reduced amounts of several extracellular virulence factors and that , independently of ClpP , ClpX is required for transcription of spa , encoding Protein A . ^^^ Here we find that the independent effect of ClpX is not general for other cell wall proteins , as expression of fibronectin and fibrinogen binding proteins was increased in the absence of either ClpX or ClpP . ^^^ To assess the roles of ClpX and ClpP within the sar / agr regulatory network , deletions in clpX and clpP were combined with mutations in these genes . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
Five clp genes ( clpC , clpB , clpP 1 , clpP 2 , and clpX ) , representing chaperone and protease encoding genes , were previously identified in Bifidobacterium breve UCC 2003 . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ClpP is a self compartmentalized proteolytic assembly comprised of two , stacked , heptameric rings that , when associated with its cognate hexameric ATPase ( ClpA or ClpX ) , form the ClpAP and ClpXP ATP dependent protease , respectively . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
ZBDs in ClpX undergo large nucleotide dependent block movement towards ClpP and into the AAA+ ring . ^^^ Evidence for this movement was initially obtained by the surprising observation that an N terminal extension on ClpX is clipped by bound ClpP in functional ClpXP complexes . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
This report describes the use of these vectors to mutate clpX and clpP of the opportunistic pathogen Pseudomonas aeruginosa and to explore their roles in biofilm formation and surface motility . ^^^
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q16740 and O76031	Pubmed	SVM Score :0.0
NA