| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.61623644 |
| In a gel shift assay , the association of FAST 2 with Smad 4 was mutually exclusive from the association with Smad 3 . 0.61623644^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.51663274 |
| Using two point mutations of Smad 2 previously identified in colorectal carcinomas , we show that Smad 2 ushers Smad 4 to the nucleus to form a transcriptional activation complex with the nuclear DNA binding protein FAST 1 and that the mutant proteins interact normally with FAST 1 but fail to recruit Smad 4 into the nucleus . 0.51663274^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.59026891 |
| FAST 1 has been shown to associate with Smad 2 and Smad 4 , transducers of TGFbeta superfamily signals , in response to stimulation by several TGFbeta superfamily ligands . 0.59026891^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| FAST 2 binds to a sequence in the gsc promoter , but efficient transcriptional activation and assembly of a DNA binding complex of FAST 2 , Smad 2 , and Smad 4 requires an adjacent Smad 4 site . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| FAST 2 also interacts directly with Smad 2 , a cytoplasmic protein which is translocated to the nucleus in response to TGF beta , and forms a multimeric complex with Smad 2 and Smad 4 on the activin response element , a high affinity binding site for FAST 1 . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| After TGF beta mediated phosphorylation and association with Smad 4 , Smad 2 moves to the nucleus and activates expression of specific genes through cooperative interactions with DNA binding proteins , including members of the winged helix family of transcription factors , forkhead activin signal transducer ( FAST ) 1 and FAST 2 . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| The hFAST 1 dependent activation of ARE was completely dependent on endogenous Smad 4 and stimulation by a TGF beta like ligand . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Smad 4 and FAST 1 in the assembly of activin responsive factor . ^^^ We now report that Smad 4 is present in ARF , and that FAST 1 , Smad 4 and Smad 2 co immunoprecipitate in a ligand regulated fashion . ^^^ In a yeast two hybrid assay , the FAST 1 carboxy terminus interacts with Smad 2 but not Smad 4 . ^^^ Deletion mutants of the FAST 1 carboxy terminus that still participate in ligand regulated Smad 2 binding no longer associated with Smad 4 or ARF . ^^^ These results indicate that Smad 4 stabilizes a ligand stimulated Smad 2 FAST 1 complex as an active DNA binding factor . . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Smad 4 is not required for nuclear translocation of Smads 1 or 2 , or for association of Smad 2 with a DNA binding partner , the winged helix protein FAST 1 . ^^^ Receptor activated Smad 2 takes Smad 4 into the nucleus where they form a complex with FAST 1 that requires these three components to activate transcription . ^^^ Smad 4 contributes two functions : Through its amino terminal domain , Smad 4 promotes binding of the Smad2 / Smad4 / FAST 1 complex to DNA ; through its carboxy terminal domain , Smad 4 provides an activation function required for Smad 1 or Smad 2 to stimulate transcription . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| In contrast , Smad 2 ( wt ) , Smad2Deltaexon3 , and Smad 3 efficiently formed ARE binding complexes with Smad 4 and FAST 1 , although Smad 2 ( wt ) did not directly bind to ARE . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| This complex contains Smad 2 or Smad 3 , Smad 4 , and a novel forkhead transcription factor , FAST 1 , and binds to an enhancer ( activin responsive element ; ARE ) that confers activin regulation of Mix . 2 transcription . ^^^ Both FAST 1 and Smads can bind directly to the ARE ; we have investigated 1 ) the role of FAST 1 and Smad DNA binding sites in ARF recognition of the ARE , 2 ) the contributions of FAST 1 and Smad binding to ARF binding in vitro and to ARE regulation in early Xenopus embryos , 3 ) the extent to which different Smads can replace Smad 4 in regulation of the ARE . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Smad 4 HL2 did not induce the activation of pAR 3 Lux , which contains FAST 1 binding sites and is activated by a complex composed of FAST 1 , Smad 2 and Smad 4 . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| All these Smad 4 variants form complexes with activated Smad 2 and Smad 3 and are incorporated into DNA binding complexes with the transcription factor Fast 1 , regardless of the amount of linker they contain . ^^^ |
|
| Interacting proteins: O75593 and Q13485 |
Pubmed |
SVM Score :0.0 |
| We show that complexes of Smad2 / Smad4 with the transcription factors Fast 1 or Fast 3 contain one Fast , two Smad2s , and one Smad 4 . ^^^ |
|