| In this study , we characterize mutations in the putative Dictyostelium discoideum orthologues of budding yeast genes that are involved in one of each of these functions , ATG 1 , ATG 6 , and ATG 8 . ^^^ As with other Dictyostelium macroautophagy mutants , development of atg 1 1 , atg 6 ( ) , and atg 8 ( ) is more aberrant in plaques on bacterial lawns than on nitrocellulose filters . ^^^ The distribution of a green fluorescent protein fusion of the autophagosome marker , Atg 8 , is aberrant in both atg 1 1 and atg 6 ( ) mutants . . ^^^ |