| Interacting proteins: O60504 and P28482 |
Pubmed |
SVM Score :0.0 |
| To evaluate the function of vinexin downstream of growth factors , we examined the effects of wild type and mutant vinexin expression on extracellular signal regulated kinase ( Erk ) and c Jun N terminal kinase / stress activated protein kinase ( JNK / SAPK ) activation in response to EGF . ^^^ Exogenous expression of vinexin beta in NIH / 3T3 cells enhanced JNK / SAPK activation but did not affect Erk activation . ^^^ |
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| Interacting proteins: O60504 and P28482 |
Pubmed |
SVM Score :0.0 |
| Vinexin beta regulates the anchorage dependence of ERK 2 activation stimulated by epidermal growth factor . ^^^ Here , we show that exogenous expression of vinexin beta , a novel focal adhesion protein , allows anchorage independent ERK 2 activation stimulated by epidermal growth factor . ^^^ In contrast , expression of vinexin beta had no effect on ERK 2 activation in adherent cells , suggesting that vinexin beta regulates the anchorage dependence of ERK 2 activation . ^^^ The dominant negative mutant of PAK suppressed the anchorage independent ERK 2 activation induced by expression of vinexin beta . ^^^ The dominant negative mutant of vinexin beta inhibited the anchorage independent ERK 2 activation induced by the PKA inhibitor . ^^^ |
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| Interacting proteins: O60504 and P28482 |
Pubmed |
SVM Score :0.0 |
| Site directed mutagenesis revealed that ERK 2 mainly phosphorylated the serine 189 residue of vinexin beta . ^^^ A putative DEF ( docking for ERK FXFP ) domain located in the linker region of vinexin was required for the interaction with ERK1 / 2 and efficient phosphorylation of vinexin beta by ERK 2 . ^^^ Finally , we showed that cell adhesion to fibronectin also induced the association of vinexin beta with ERK 2 and the phosphorylation of vinexin beta . ^^^ Furthermore , vinexin and ERK were co localized to the periphery of cells during cell spreading on fibronectin . ^^^ Together , these results suggest that vinexin is a novel substrate of ERK 2 and may play roles in ERK dependent cell regulation during cell spreading as well as in growth factor induced responses . . ^^^ |
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| Interacting proteins: O60504 and P28482 |
Pubmed |
SVM Score :0.0 |
| Functional studies with C3H10T1 / 2 cells showed that Vinexin gamma acted as a scaffold protein to activate MEK and ERK through interaction with c Raf and ERK . ^^^ The phenotype displayed by the mice indicated that ERK activation was decreased in the Vinexin gamma ( / ) XY gonads , and Sox 9 expression was down regulated . ^^^ |
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