| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.68974331 |
| CD2AP is expressed with nephrin in developing podocytes and is found widely in mature kidney and elsewhere . 0.68974331^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :1.3022751 |
| In addition , nephrin was found to partially colocalize with CD2AP and podocin in double immunofluorescence microscopy , confirming the close proximity of these proteins and proposing that these proteins may belong to nephrin associated protein complex in glomeruli . 1.3022751^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :1.0335065 |
| In contrast , podocin and CD2AP , two proteins shown to interact with nephrin in the slit diaphragm , are acutely downregulated at days 3 7 and , thereafter , recovered again to normal levels after 29 days . 1.0335065^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| It is reported that podocin interacts with nephrin and CD2AP , and that NEPH 1 interacts with nephrin . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Supporting a role for CD2AP in the specialized cell junction known as the slit diaphragm , CD2AP associated with nephrin , the primary component of the slit diaphragm . . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| In particular , two proteins , CD 2 associated protein and nephrin , have been identified as critical podocyte proteins that are both required for normal glomerular filtration . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| However , more recent studies on nephrin , a key component of the slit diaphragm , as well as the podocyte and slit diaphragm associated intracellular proteins , CD 2 associated protein , podocin and alpha actinin 4 , have emphasized the role of the slit diaphragm as a central size selective filtration barrier . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| The identification of nephrin , a component of the slit diaphragm , and the intracellular slit diaphragm associated proteins CD2AP and podocin has demonstrated the existence of proteins that directly contribute to a functional kidney filter . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| In addition to nephrin , other podocyte proteins ( podocin , alpha actinin 4 , CD2AP , FAT ) have recently been identified and associated with the development of proteinuria . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| CD2AP localizes to the slit diaphragm and binds to nephrin via a novel C terminal domain . ^^^ Here we demonstrate that CD2AP localizes to the slit diaphragm in podocytes using immunoelectron microscopy and that nephrin and CD2AP co immunoprecipitate from a podocyte cell line . ^^^ The specificity of this interaction was verified by mapping studies , which demonstrated that a novel domain at the C terminus of CD2AP interacts with the C terminal portion of the nephrin cytoplasmic domain . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Podocin , a raft associated component of the glomerular slit diaphragm , interacts with CD2AP and nephrin . ^^^ Moreover , GST pull down experiments reveal that podocin associates via its COOH terminal domain with CD2AP , a cytoplasmic binding partner of nephrin , and with nephrin itself . ^^^ That podocin interacts with CD2AP and nephrin in vivo is shown by coimmunoprecipitation of these proteins from glomerular extracts . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| After transfer to the `` nonpermissive ' ' temperature ( 37 degrees C ) , they entered growth arrest and expressed markers of differentiated in vivo podocytes , including the novel podocyte proteins , nephrin , podocin , CD2AP , and synaptopodin , and known molecules of the slit diaphragm ZO 1 , alpha , beta , and gamma catenin and P cadherin . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| CD 2 associated protein ( CD2AP ) is also necessary for normal glomerular permeability and is a putative nephrin adapter molecule . ^^^ Here , we document that nephrin and CD2AP are linked to the actin cytoskeleton . ^^^ As detected by Western blot analysis , nephrin and CD2AP were both insoluble when cell membranes from normal rat glomeruli were extracted with 0 . 5 % Triton 10 100 ( TX 100 ) at 4 degrees C in the presence of divalent cations , but they were solubilized when the extraction included potassium iodide ( KI ) to depolymerize F actin . ^^^ In addition , a small fraction of the solubilized nephrin and CD2AP was recovered in the low density fractions of OptiPrep flotation gradients , which indicates that a portion of nephrin , possibly associated with CD2AP , resides in a cholesterol or sphingolipid rich region of the plasma membrane . ^^^ Immunofluorescent staining of unfixed sections of normal rat kidney for nephrin , CD2AP , and F actin was unaltered by treatment with TX 100 but was greatly diminished by addition of KI . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Immunogold electron microscopy confirmed the progressive disappearance of nephrin from podocyte foot processes and retention of CD2AP . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| The immunohistochemical staining showed also that the interaction partner of nephrin , CD 2 associated protein ( CD2AP ) , and the slit diaphragm associated protein , ZO 1alpha ( ) , appeared unchanged , whereas the major anionic apical membrane protein of podocytes , podocalyxin , somewhat punctate as compared with the wild type ( wt ) and nephrin ( wt / trap ) stainings . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| CD 2 associated protein ( CD2AP ) is an adapter protein associating with several membrane proteins , including nephrin , mutated in congenital nephrotic syndrome of the Finnish type , and polycystin 2 , mutated in type 2 autosomal dominant polycystic kidney disease . ^^^ The present results suggest that CD2AP is involved in the regulation of the actin cytoskeleton and indicate that CD2AP may act as a direct adapter between the actin cytoskeleton and cell membrane proteins , such as nephrin and polycystin 2 . ^^^ CD 2 associated protein ( CD2AP ) is an adapter protein associating with several membrane proteins , including nephrin , mutated in congenital nephrotic syndrome of the Finnish type , and polycystin 2 , mutated in type 2 autosomal dominant polycystic kidney disease . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| We describe in a human podocyte cell line , the subcellular distribution of nephrin , podocins , and CD2AP and their functional interaction with the cytoskeleton . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 co immunoprecipitated with the podocyte slit diaphragm proteins nephrin and CD2AP , and dual immunofluorescence confirmed co localization of caveolin 1 and nephrin . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| The importance of several of them ( nephrin , podocin , CD2AP , and Neph 1 ) in the maintenance of the glomerular filtration barrier has been demonstrated by the occurrence of massive proteinuria when they are defective . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Regions examined were at human chromosome 10p , 10q ( orthologous to the rat renal susceptibility Rf 1 locus ) , and at NPHS 1 ( nephrin ) , CD2AP , Wilms tumor ( WT 1 ) , and NPHS 2 ( podocin ) loci . ^^^ We have excluded linkage with candidate regions for nephrin , CD2AP , WT 1 , and podocin in this sample . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Nephrin and CD2AP associate with phosphoinositide 3 OH kinase and stimulate AKT dependent signaling . ^^^ Mutations of NPHS 1 or NPHS 2 , the genes encoding nephrin and podocin , as well as the targeted disruption of CD 2 associated protein ( CD2AP ) , lead to heavy proteinuria , suggesting that all three proteins are essential for the integrity of glomerular podocytes , the visceral glomerular epithelial cells of the kidney . ^^^ We demonstrate that both nephrin and CD2AP interact with the p 85 regulatory subunit of phosphoinositide 3 OH kinase ( PI3K ) in vivo , recruit PI3K to the plasma membrane , and , together with podocin , stimulate PI3K dependent AKT signaling in podocytes . ^^^ One such target is Bad ; its phosphorylation and inactivation by 14 3 3 protects podocytes against detachment induced cell death , suggesting that the nephrin CD2AP mediated AKT activity can regulate complex biological programs . ^^^ Our findings reveal a novel role for the slit diaphragm proteins nephrin , CD2AP , and podocin and demonstrate that these three proteins , in addition to their structural functions , initiate PI3K / AKT dependent signal transduction in glomerular podocytes . . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The identification of nephrin and CD2AP , podocyte proteins which modulate the properties of glomerular barrier selectivity , has made it possible to unravel the mechanisms undergoing foot process effacement and proteinuria . ^^^ Here we explored the role of nephrin and CD2AP together with the integrity of the slit diaphragm in the pathogenesis of proteinuria in patients with acquired glomerular diseases . ^^^ The staining of intracellular nephrin and CD2AP did not change among patients . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Protein levels of nephrin , podocin , CD 2 associated protein , and podocalyxin were investigated using quantitative immunohistochemical assays . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Glomerulosclerosis was a relatively late event , accompanied by severe albuminuria and loss of WT 1 , nephrin , CD2AP , and alpha actinin 4 . . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Changes in expression of slit pore constituents ( podocin , CD2AP , nephrin and ZO 1 ) , cytoskeleton associated proteins ( actin , alpha actinin , ezrin and synaptopodin ) , the GDH podocyte adhesion molecules alpha ( 3 ) integrin , and heparan sulfate were studied by immunofluorescence . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| CD2AP localises to the slit diaphragm and links nephrin and podocin to phosphoinositide 3 OH kinase ; this complex has cell signalling properties . ^^^ Transfection of podocytes with mutated CD2AP or study of cultured podocytes from CD2AP + / mice would provide further insight into whether the nephrin podocin CD2AP signal transduction pathway is altered and leads to increased apoptosis of podocytes . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Using real time PCR and immunolabeling , we showed that the expression of other slit diaphragm components was modified in Nphs 2 / kidneys : the expression of the nephrin gene was downregulated , whereas that of the ZO 1 and CD2AP genes appeared to be upregulated . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Several molecules , including nephrin , CD2AP , FAT , ZO 1 , P cadherin , Podocin , and Neph 1 3 have all been shown to be associated with the SD complex , and some of these molecules are critical for its integrity . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Here we addressed the role of nephrin , CD 2 associated protein ( CD2AP ) , and podocin together with the integrity of the slit diaphragm in the pathogenesis of proteinuria of patients with diabetes and nephropathy . ^^^ Reduction of nephrin in the context of normal expression of CD2AP and podocin can be taken reasonably as a specific marker of renal disease in diabetes . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Intriguingly , nephrin and actin associated proteins ( synaptopodin , CD2AP , and cortactin ) were recruited to and accumulated at the entire cell margin only in confluent cells , but not in dispersed cells . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Podocin is a protein associated with the slit diaphragm that interacts with nephrin and CD 2 associated protein ( CD2AP ) within lipid rafts . ^^^ In all cases , podocin defect was associated with changes in the distribution of nephrin , CD2AP , and alpha actinin : the proteins were mainly detected in the podocyte body , with mild expression along the GBM . ^^^ Secondary changes in the distribution of nephrin , CD2AP , and alpha actinin are additional evidences for the scaffolding role of podocin in the organization of the slit diaphragm . . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Using co immunoprecipitation and pull down assays we show here that nephrin forms a multiprotein complex with cadherins and p 120 catenin and with three scaffolding proteins , ZO 1 , CD2AP , and CASK , in kidney glomeruli and when expressed in Madin Darby canine kidney cells . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| The exact mechanism by which nephrin controls permselectivity is not yet clear , but it is known to interact with several podocyte proteins including CD2AP , podocin , and alpha actinin 4 . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| CD2AP , podocin , Fyn kinase , and phosphoinositide 3 kinase are reported intracellular interacting partners of nephrin , although the biological roles of these interactions are unclarified . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Podocytes are critical in maintaining the filtration barrier of the glomerulus and are dependent on the slit diaphragm ( SD ) proteins nephrin , podocin , and CD 2 associated protein ( CD2AP ) to function optimally . ^^^ With the use of a conditionally immortalized human podocyte cell line , it first was shown that exposure to normal and non nephrotic human plasma leads to a concentration of nephrin , podocin , CD2AP , and actin at the cell surface . ^^^ When exposed to all nephrotic plasma samples ( and a non human serum control ) , nephrin podocin and CD2AP assumed a cytoplasmic distribution ; nephrin and synaptopodin were selectively downregulated , and the relocation of nephrin induced by nephrotic plasma could be rescued back to the plasma membrane by co incubation with non nephrotic plasma . ^^^ Nephrotic plasma alters slit diaphragm dependent signaling and translocates nephrin , Podocin , and CD 2 associated protein in cultured human podocytes . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| METHODS : The expression and the localization of slit diaphragm associated molecules ( nephrin , podocin , and CD2AP ) and other podocyte associated molecules ( podocalyxin and alpha ( 3 ) integrin ) in passive and active Heymann nephritis were analyzed by immunofluorescence and Western blot analysis . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Also , the genes encoding the four major slit diaphragm proteins , nephrin , podocin , Neph 1 and CD 2 associated protein were sequenced in 38 patients with MCNS of varying severity . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Together with podocin and CD2AP ( CD 2 associated protein ) , nephrin forms a complex determining the integrity of the slit diaphragm . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Mounting evidence supports an expanding role of the slit diaphragm in signal transduction to mediate downstream cellular responses , including prosurvival effects of the integral proteins nephrin and CD2AP . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| The relationship among nephrin , podocin , CD2AP , and alpha actinin might not be a true ' interaction ' in podocyte . ^^^ We respectively knockdown the nephrin , podocin , CD2AP , or alpha actinin 4 mRNA by using reconstructed RNA interference vector psiRNA hH1GFPzeo in mouse podocyte clone . ^^^ With nephrin knockdown , only CD2AP increased , whereas podocin showed no change . ^^^ Contrarily , with podocin or CD2AP knockdown , nephrin decreased , while CD2AP or podocin increased . ^^^ Nephrin , podocin , or CD2AP knockdown did not change the expression of alpha actinin 4 , whereas alpha actinin 4 knockdown begetted the reduction of nephrin , and the increment of podocin and CD2AP . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Sema 3A induced a dose response podocin downregulation and decreased its interaction with CD 2 associated protein and nephrin , as determined by Western analysis and co immunoprecipitation . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Nephrin , podocin , CD2AP , and alpha actinin 4 are important podocyte proteins that help maintain the integrity of the slit diaphragm and prevent proteinuria . ^^^ In this study , changes in the expression and distribution of nephrin , podocin , CD2AP , and alpha actinin 4 were dynamically detected in Adriamycin induced nephrotic ( ADR ) rats treated with three different drugs : lisinopril , prednisone , and ATRA . ^^^ The distribution and the expression of messenger RNA and protein of nephrin , podocin , CD2AP , and alpha actinin 4 were detected by indirect immunofluorescence , real time polymerase chain reaction , and Western blotting , respectively . ^^^ With the intervention of lisinopril , prednisone , and ATRA , changes in the expression of nephrin , podocin , and CD2AP were diverse , which was different from that detected in ADR rats . ^^^ After lisinopril and prednisone intervention , podocin exhibited prominent earlier changes compared with those of nephrin and CD2AP , whereas CD2AP showed more prominent changes after ATRA intervention . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Therefore , to investigate the mechanism of Wt 1 R394W induced renal failure , the expression of genes whose deletion leads to glomerulosclerosis ( NPHS 1 , NPHS 2 , and CD2AP ) was quantitated . ^^^ However , the data do suggest that Wt 1 R394W induced glomerulosclerosis may be independent of downregulation of the genes for NPHS 1 , NPHS 2 , CD2AP , and podocalyxin and may involve other genes yet to be implicated in renal failure . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| TERalb ( % / h ) was similar in normoalbuminuric and microalbuminuric group 1 , 2 , and 3 diabetic patients ( medians : 14 . 1 vs . 14 . 4 vs . 15 . 7 vs . 14 . 9 , respectively ) ( ANOVA , NS ) . mRNA expression of slit diaphragm proteins CD2AP , FAT , Actn 4 , NPHS 1 , and NPHS 2 was higher in normoalbuminuric patients than in microalbuminuric patients ( groups 1 , 2 , and 3 ) ( ANOVA , P < 0 . 001 ) . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Real time polymerase chain reaction measurement of mRNA SD proteins ( CD2AP , FAT , Actn 4 , NPHS 1 , and NPHS 2 ) significantly increased in kidney biopsy specimens after simvastatin , but not cholestyramine treatment . ^^^ |
|
| Interacting proteins: O60500 and Q9Y5K6 |
Pubmed |
SVM Score :0.0 |
| Seven genes have been recognized till present , which mutations are responsible for severe forms of NS : NPHS 1 , NPHS 2 , ACTN 4 , CD2AP and WT 1 , TRPC 6 , LAMB 2 . ^^^ |
|