| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| However , more recent studies on nephrin , a key component of the slit diaphragm , as well as the podocyte and slit diaphragm associated intracellular proteins , CD 2 associated protein , podocin and alpha actinin 4 , have emphasized the role of the slit diaphragm as a central size selective filtration barrier . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Interaction with podocin facilitates nephrin signaling . ^^^ Mutations of NPHS 1 or NPHS 2 , the genes encoding for the glomerular podocyte proteins nephrin and podocin , cause steroid resistant proteinuria . ^^^ Nephrin induced signaling is greatly enhanced by podocin , which binds to the cytoplasmic tail of nephrin . ^^^ Mutational analysis suggests that abnormal or inefficient signaling through the nephrin podocin complex contributes to the development of podocyte dysfunction and proteinuria . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The identification of nephrin , a component of the slit diaphragm , and the intracellular slit diaphragm associated proteins CD2AP and podocin has demonstrated the existence of proteins that directly contribute to a functional kidney filter . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| In addition to nephrin , other podocyte proteins ( podocin , alpha actinin 4 , CD2AP , FAT ) have recently been identified and associated with the development of proteinuria . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Mutations in podocyte proteins , such as nephrin , alpha actinin 4 , and podocin , are associated with proteinuria and nephrotic syndrome . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Mutations of the novel renal glomerular genes NPHS 1 and NPHS 2 encoding nephrin and podocin cause two types of severe nephrotic syndrome presenting in early life , Finnish type congenital nephrotic syndrome ( CNF ) and a form of autosomal recessive familial focal segmental glomerulosclerosis ( SRN 1 ) , respectively . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression . ^^^ After transfer to the `` nonpermissive ' ' temperature ( 37 degrees C ) , they entered growth arrest and expressed markers of differentiated in vivo podocytes , including the novel podocyte proteins , nephrin , podocin , CD2AP , and synaptopodin , and known molecules of the slit diaphragm ZO 1 , alpha , beta , and gamma catenin and P cadherin . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| In this regard , the human mutations in nephrin , podocin , alpha actinin 4 , COL4A3 , and COL4A5 genes expressed in the glomeruli have been implicated to cause alterations in glomerular filtration apparatus . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The molecules studied included the filtration slit component nephrin , the hairpin like membrane protein podocin , the basolateral adhesion molecules beta 1 integrin and alpha dystroglycan , and the cytoskeleton linking intermediary beta catenin and the actin associated alpha actinin 4 . ^^^ The results showed diminished protein levels of podocin and nephrin in the PA treated group . beta catenin showed distinct down regulation at 3 days of induction , and the control level was reached at 10 days . beta 1 integrin was markedly up regulated during induction . alpha actinin 4 was not changed at the studied time points . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The levels of Podocin , the gene mutated in autosomal recessive steroid resistant nephrotic syndrome ( NPHS 2 ) , and Nephrin , the gene mutated in congenital nephrotic syndrome of the Finnish type ( NPHS 1 ) , are slightly reduced in kr ( enu ) / kr ( enu ) podocytes . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Co localization of nephrin , podocin , and the actin cytoskeleton : evidence for a role in podocyte foot process formation . ^^^ The discovery of the genes for nephrin and podocin , which are mutated in two types of congenital nephrotic syndrome , was pivotal in establishing the podocyte as the central component of the glomerular filtration barrier . ^^^ In addition to membrane expression , nephrin and podocin were detected intracellularly in a filamentous pattern . ^^^ Double immunolabeling and depolymerization studies showed that nephrin and podocin partially co localize with actin , most strikingly seen protruding from the tips of actin filaments , and are dependent on intact actin polymers for their intracellular distribution . ^^^ We demonstrate an intimate relationship between nephrin podocin and filamentous actin , and reason that disruption of nephrin / podocin could be a final common pathway leading to foot process effacement in proteinuric diseases . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Nevertheless , in caveolin 1 deficient mice , podocyte ultrastructure appeared normal , and the podocyte proteins synaptopodin , nephrin , and podocin were expressed normally . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Mutations of NPHS 1 or NPHS 2 , the genes encoding for the glomerular podocyte proteins nephrin and podocin , cause steroid resistant proteinuria . ^^^ Podocin interacts with the C terminal domain of nephrin and facilitates nephrin dependent signaling . ^^^ We conclude that NEPH 1 defines a new family of podocin binding molecules that are potential candidates for hereditary nephrotic syndromes not linked to either NPHS 1 or NPHS2 . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| We used in vitro translated podocin and purified nephrin to investigate the effect of R229Q on their interaction and found decreased nephrin binding to the R229Q podocin . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| A rat homologue of podocin was cloned , and the expression of podocin was investigated and then compared with the nephrin and the ZO 1 expressions in rat experimental proteinuric models and in developing glomeruli . ^^^ The localization of podocin has close proximity to that of nephrin in normal adult rat glomeruli . ^^^ Podocin staining was restricted to the basal side of the podocyte of the early developing stage , whereas nephrin staining was detected on the basolateral surface of podocyte . ^^^ The redistribution of podocin was observed in the anti nephrin antibody ( ANA ) induced nephropathy and puromycin aminonucleoside ( PAN ) nephropathy . ^^^ The redistribution of podocin paralleled with nephrin in ANA nephropathy but not in PAN nephropathy . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Recently , identification of the mutated genes for some podocyte proteins ( nephrin , podocin , alpha actinin 4 ) in rare familial forms of nephrotic syndrome shed has new light on the molecular mechanisms of glomerular permselectivity . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Like Nephrin and Podocin , Neph 1 was enriched in Triton 10 100 detergent resistant membrane fractions . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Mutation of the basic structural protein of slit diaphragm , nephrin , results in the Finnish type of the congenital nephrotic syndrome , mutations of other podocyte proteins , e . g . podocin , or alpha actinin 4 result in congenital focal segmental glomerulosclerosis . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Significant advances have been made in understanding the complex and interacting role of nephrin and podocin mutations in the genesis of clinical glomerular disease . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| All carriers of heterozygous coding podocin mutation or R229Q were screened for nephrin mutation that was found in heterozygosity associated with R229Q in one patient . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The importance of several of them ( nephrin , podocin , CD2AP , and Neph 1 ) in the maintenance of the glomerular filtration barrier has been demonstrated by the occurrence of massive proteinuria when they are defective . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Regions examined were at human chromosome 10p , 10q ( orthologous to the rat renal susceptibility Rf 1 locus ) , and at NPHS 1 ( nephrin ) , CD2AP , Wilms tumor ( WT 1 ) , and NPHS 2 ( podocin ) loci . ^^^ We have excluded linkage with candidate regions for nephrin , CD2AP , WT 1 , and podocin in this sample . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Mutations of NPHS 1 or NPHS 2 , the genes encoding nephrin and podocin , as well as the targeted disruption of CD 2 associated protein ( CD2AP ) , lead to heavy proteinuria , suggesting that all three proteins are essential for the integrity of glomerular podocytes , the visceral glomerular epithelial cells of the kidney . ^^^ We demonstrate that both nephrin and CD2AP interact with the p 85 regulatory subunit of phosphoinositide 3 OH kinase ( PI3K ) in vivo , recruit PI3K to the plasma membrane , and , together with podocin , stimulate PI3K dependent AKT signaling in podocytes . ^^^ Our findings reveal a novel role for the slit diaphragm proteins nephrin , CD2AP , and podocin and demonstrate that these three proteins , in addition to their structural functions , initiate PI3K / AKT dependent signal transduction in glomerular podocytes . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Pathogenesis of proteinuria : lessons learned from nephrin and podocin . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Protein levels of nephrin , podocin , CD 2 associated protein , and podocalyxin were investigated using quantitative immunohistochemical assays . ^^^ Real time PCR was used to determine the mRNA levels of nephrin , podocin , and podoplanin in microdissected glomeruli . ^^^ In most acquired renal diseases , except in IgA nephropathy , a marked reduction was observed at the protein levels of nephrin , podocin , and podocalyxin , whereas an increase of the glomerular mRNA levels of nephrin , podocin , and podoplanin was found , compared with controls . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Expression of nephrin , podocin , alpha actinin , and WT 1 in children with nephrotic syndrome . ^^^ Recently , nephrin , podocin , alpha actinin , and WT 1 , which are located at the slit diaphragm and expressed by the podocyte , were found to be causative in congenital / familial nephrotic syndrome ( NS ) , but their role in acquired NS remains unclear . ^^^ Furthermore , we also found the pattern of distribution of nephrin , podocin , and alpha actinin changed in children with NS . ^^^ In conclusion , a dramatic decrease of podocin expression and abnormal distribution of nephrin , podocin , and alpha actinin were found in children with NS . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Cells obtained in the urine from PHN rats were positive for synaptopodin , nephrin , podocin , WT 1 , and GLEPP 1 ( podocyte specific antigens ) . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| At least three genes have been identified which , when defective , cause familial FSGS or nephrosis : the NPHS 1 gene , encoding nephrin ; the NPHS 2 gene , encoding podocin ; and the ACTN 4 gene , encoding a actinin 4 . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| For identifying potential diagnostic markers of proteinuric glomerulopathies , glomerular mRNA levels of molecules relevant for podocyte function ( alpha actinin 4 , glomerular epithelial protein 1 , Wilms tumor antigen 1 , synaptopodin , dystroglycan , nephrin , podoplanin , and podocin ) were determined by quantitative real time RT PCR from microdissected glomeruli . ^^^ However , a significant positive correlation between alpha actinin 4 , glomerular epithelial protein 1 , synaptopodin , dystroglycan , Wilms tumor antigen 1 , and nephrin was found in all analyzed glomeruli , whereas podocin mRNA expression did not correlate . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Molecular basis of the functional podocin nephrin complex : mutations in the NPHS 2 gene disrupt nephrin targeting to lipid raft microdomains . ^^^ Mutations of NPHS 1 or NPHS 2 , the genes encoding for nephrin and podocin , lead to early onset of heavy proteinuria , and rapid progression to end stage renal disease , suggesting that both proteins are essential for the integrity of the glomerular filter . ^^^ The association of podocin with specialized lipid raft microdomains of the plasma membrane was a prerequisite for recruitment of nephrin into rafts . ^^^ In contrast , disease causing mutations of podocin ( R138Q and R138X ) failed to recruit nephrin into rafts either because these mutants were retained in the endoplasmic reticulum ( R138Q ) , or because they failed to associate with rafts ( R138X ) despite their presence in the plasma membrane . ^^^ Our findings demonstrate that the failure of mutant podocin to recruit nephrin into lipid rafts may be essential for the pathogenesis of NPHS2 . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Changes in expression of slit pore constituents ( podocin , CD2AP , nephrin and ZO 1 ) , cytoskeleton associated proteins ( actin , alpha actinin , ezrin and synaptopodin ) , the GDH podocyte adhesion molecules alpha ( 3 ) integrin , and heparan sulfate were studied by immunofluorescence . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The monoclonal anti IP 10 antibody treatment decreased the expression of IP 10 and podocyte associated proteins such as nephrin and podocin that are reported to be essential for maintaining the podocyte function ( IP 10 , 53 . 0 % to control ; nephrin , 43 . 5 % ; podocin , 60 . 4 % ) . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Nephrin and podocin are slit diaphragm proteins identified in families with congenital nephrotic syndromes . ^^^ CD2AP localises to the slit diaphragm and links nephrin and podocin to phosphoinositide 3 OH kinase ; this complex has cell signalling properties . ^^^ Transfection of podocytes with mutated CD2AP or study of cultured podocytes from CD2AP + / mice would provide further insight into whether the nephrin podocin CD2AP signal transduction pathway is altered and leads to increased apoptosis of podocytes . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Podocyte specific antigens , including WT 1 , synaptopodin , nephrin , and podocin , were not expressed by any cells in glomerular crescents , suggesting that podocytes underwent profound phenotypic changes in this nephritis model . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Several molecules , including nephrin , CD2AP , FAT , ZO 1 , P cadherin , Podocin , and Neph 1 3 have all been shown to be associated with the SD complex , and some of these molecules are critical for its integrity . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| This topic is of particular interest in light of the rapidly growing body of literature regarding mutations of proteins such as nephrin and podocin that are expressed at or near the podocyte slit diaphragm . ^^^ RECENT FINDINGS : The phenotypic variance of patients with congenital nephrotic syndrome with nephrin and podocin mutations resulting from triallelic mutations represents an important advance in our understanding of the effect of multiple genetic mutations on clinical disease expression . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The expressions of podocin , nephrin , alpha actinin and WT 1 in glomeruli of the proband were detected by indirect immunofluorescence . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Here we addressed the role of nephrin , CD 2 associated protein ( CD2AP ) , and podocin together with the integrity of the slit diaphragm in the pathogenesis of proteinuria of patients with diabetes and nephropathy . ^^^ Reduction of nephrin in the context of normal expression of CD2AP and podocin can be taken reasonably as a specific marker of renal disease in diabetes . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Podocin is a protein associated with the slit diaphragm that interacts with nephrin and CD 2 associated protein ( CD2AP ) within lipid rafts . ^^^ In all cases , podocin defect was associated with changes in the distribution of nephrin , CD2AP , and alpha actinin : the proteins were mainly detected in the podocyte body , with mild expression along the GBM . ^^^ Secondary changes in the distribution of nephrin , CD2AP , and alpha actinin are additional evidences for the scaffolding role of podocin in the organization of the slit diaphragm . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Nephrin , podocin and alpha actinin are all involved in proteinuria , but it is unclear which molecular event plays a crucial role during the development of proteinuria . ^^^ Two days after PAN injection , nephrin and podocin staining became discontinuous , podocin intensity decreased and FP swelled . ^^^ Both podocin and nephrin intensity decreased dramatically when heavy proteinuria occurred , but nephrin mRNA was regained . ^^^ When proteinuria disappeared , podocin recovered whereas nephrin did not ( P = 0 . 02 ) ; alpha actinin intensity increased ( P = 0 . 009 ) and the distribution changed . ^^^ The podocyte FP volume density correlated negatively with nephrin ( r = 0 . 78 , P = 0 . 0001 ) and podocin immunofluorescence intensity ( r = 0 . 76 , P = 0 . 0001 ) . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Podocin is a membrane integrated protein that is located at the glomerular slit diaphragm and directly interacts with nephrin . ^^^ Confocal microscopy was applied to determine subcellular localization of the wild type and the mutated podocin molecules , as well as wild type nephrin in transfected cells . ^^^ Immunoprecipitation and pull down studies were carried out to investigate the molecular interaction of podocin mutants and wild type nephrin . ^^^ Interestingly , this abnormal subcellular localization of podocin missense mutants also resulted in alteration in protein trafficking of wild type nephrin in cotransfected cells through the strong protein binding between both molecules . ^^^ CONCLUSION : In patients with SRN , some missense mutations in the NPHS 2 gene not only lead to misfolding and mislocalization of the mutated podocin , but they can also interfere with slit diaphragm structure and function by altering the proper trafficking of nephrin to the plasma membrane . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The exact mechanism by which nephrin controls permselectivity is not yet clear , but it is known to interact with several podocyte proteins including CD2AP , podocin , and alpha actinin 4 . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Preliminary molecular screenings for genes encoding proteins of the slit diaphragm ( eg , podocin , nephrin , alpha actinin ) were performed to exclude inherited forms of sporadic SRNS . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The electrophysiologic properties of a conditionally immortalized human podocyte cell line that expresses the specific podocyte proteins nephrin , podocin , and synaptopodin were examined by patch clamp . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| All cells from diabetic rats stained positive for the podocyte specific proteins synaptopodin , nephrin , podocin and Glepp 1 and negative for mesangial ( OX 7 ) , tubular ( Tamm Horsfall protein ) and endothelial ( RECA ) cell antigens . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| CD2AP , podocin , Fyn kinase , and phosphoinositide 3 kinase are reported intracellular interacting partners of nephrin , although the biological roles of these interactions are unclarified . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Nephrotic plasma alters slit diaphragm dependent signaling and translocates nephrin , Podocin , and CD 2 associated protein in cultured human podocytes . ^^^ Podocytes are critical in maintaining the filtration barrier of the glomerulus and are dependent on the slit diaphragm ( SD ) proteins nephrin , podocin , and CD 2 associated protein ( CD2AP ) to function optimally . ^^^ With the use of a conditionally immortalized human podocyte cell line , it first was shown that exposure to normal and non nephrotic human plasma leads to a concentration of nephrin , podocin , CD2AP , and actin at the cell surface . ^^^ When exposed to all nephrotic plasma samples ( and a non human serum control ) , nephrin podocin and CD2AP assumed a cytoplasmic distribution ; nephrin and synaptopodin were selectively downregulated , and the relocation of nephrin induced by nephrotic plasma could be rescued back to the plasma membrane by co incubation with non nephrotic plasma . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Nephrin and podocin expression around the onset of puromycin aminonucleoside nephrosis . ^^^ Decreased expression levels of the glomerular slit membrane proteins , nephrin and podocin , have been reported after the onset of puromycin aminonucleoside ( PA ) nephrosis . ^^^ We examined nephrin and podocin expressions prior to the onset of proteinuria of PA nephrosis to elucidate the proteinuria induction mechanism of PA . ^^^ The mRNA levels of nephrin and podocin in whole kidney total RNA were quantified by the TaqMan real time PCR quantification system . ^^^ The localization and levels of nephrin and podocin molecules were analyzed by immunofluorescence and Western blotting , respectively . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Mutations of NPHS 1 and NPHS 2 , which encode the slit diaphragm components nephrin and podocin , cause CNS and autosomal recessive familial steroid resistant nephrotic syndrome , respectively . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| During the calcium switch , there were reversible changes in localization and detergent solubility of the slit diaphragm protein ZO 1 and alpha actinin 4 , whereas nephrin and podocin solubility were unchanged . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Nephrin and podocin dissociate at the onset of proteinuria in experimental membranous nephropathy . ^^^ METHODS : The expression and the localization of slit diaphragm associated molecules ( nephrin , podocin , and CD2AP ) and other podocyte associated molecules ( podocalyxin and alpha ( 3 ) integrin ) in passive and active Heymann nephritis were analyzed by immunofluorescence and Western blot analysis . ^^^ RESULTS : Shifts in nephrin and podocin staining patterns , from linear to granular , were detected in the early stages of passive Heymann nephritis . ^^^ Decreased mRNA expression of nephrin and podocin was observed before the onset of proteinuria . ^^^ CONCLUSION : Nephrin is dissociated from podocin and excreted into urine in the early stages of Heymann nephritis . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Glomerular abundance of nephrin and podocin in experimental nephrotic syndrome : different effects of antiproteinuric therapies . ^^^ Although the mechanisms underlying this phenomenon are not yet fully clarified , it is well accepted that nephrin and podocin are involved in the development of proteinuria . ^^^ The effects of early treatment with various antiproteinuric therapies on proteinuria and glomerular staining of nephrin and podocin in rats with experimental NS have not been previously studied . ^^^ Proteinuria and glomerular nephrin and podocin immunofluorescence were examined in rat kidneys with adriamycin induced NS and the effects of antiproteinuric drug therapies during 5 wk with enalapril , losartan , alone or in combination , omapatrilat , and mycophenolate mofetil on these parameters were assessed . ^^^ Nephrotic rats exhibited severe disruption of slit diaphragm structure as seen by rapid and profound loss of nephrin and podocin . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Direct effect of plasma permeability factors from patients with idiopatic FSGS on nephrin and podocin expression in human podocytes . ^^^ Since these patients show reduced nephrin and podocin expression at renal biopsy , we evaluated the effect of serum and PF from patients with FSGS on nephrin and podocin expression in human podocytes . ^^^ Nephrin and podocin expression was semi quantitatively evaluated by immunofluorescence . ^^^ Our results demonstrate that serum and PF from FSGS patients may directly affect nephrin and podocin in human podocytes , thus providing new insights into the mechanisms causing proteinuria in FSGS . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| ATRA induces podocyte differentiation and alters nephrin and podocin expression in vitro and in vivo . ^^^ RESULTS : ATRA induced podocyte process formation in vitro , and significantly increased the expression of nephrin and podocin . ^^^ ATRA also significantly prevented the decrease in staining for synaptopodin , nephrin , and podocin in experimental animals ( P < 0 . 05 vs . control ) . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Also , the genes encoding the four major slit diaphragm proteins , nephrin , podocin , Neph 1 and CD 2 associated protein were sequenced in 38 patients with MCNS of varying severity . ^^^ The genetic analyses revealed heterozygous amino acid changes in nephrin and podocin in 10 of the 38 patients studied . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The mRNA expressions of nephrin ( NephRNA ) , podocin ( PodRNA ) and synaptopodin ( SynRNA ) in urinary sediment were measured by real time quantitative PCR . ^^^ CONCLUSIONS : Urinary mRNA expression of podocyte markers , such as nephrin and podocin , are significantly different between proteinuric disease categories . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The main components of the slit diaphragm are nephrin , the product of NPHS 1 gene and podocin , the product of NPHS 2 gene . ^^^ Reduced expression and redistribution of nephrin and podocin are also seen in podocytes of patients with acquired glomerulopathies . ^^^ Together with podocin and CD2AP ( CD 2 associated protein ) , nephrin forms a complex determining the integrity of the slit diaphragm . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Organization of the pronephric filtration apparatus in zebrafish requires Nephrin , Podocin and the FERM domain protein Mosaic eyes . ^^^ We first characterized the function of the zebrafish homolog of Nephrin , the disease gene associated with the congenital nephritic syndrome of the Finnish type , and Podocin , the gene mutated in autosomal recessive steroid resistant nephrotic syndrome . ^^^ Zebrafish nephrin and podocin were specifically expressed in pronephric podocytes and required for the development of pronephric podocyte cell structure . ^^^ Ultrastructurally , disruption of nephrin or podocin expression resulted in a loss of slit diaphragms at 72 and 96 h post fertilization and failure to form normal podocyte foot processes . ^^^ A functional assay of glomerular filtration barrier revealed that absence of normal nephrin , podocin or mosaic eyes expression results in loss of glomerular filtration discrimination and aberrant passage of high molecular weight substances into the glomerular filtrate . . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The increase in UAE in the diabetes group was associated with a significant reduction in the expression of slit diaphragm associated molecules compared with control ( nephrin ; P < 0 . 05 and podocin ; P < 0 . 005 ) that was reversed by ATL146e treatment . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| In cultured podocytes subjected to recombinant IP 10 treatment , the expression of slit diaphragm ( SD ) components nephrin and podocin clearly was heightened . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Immunofluorescence studies of podocyte associated proteins nephrin and podocin revealed diminished and discontinuous staining patterns in rats with PAN nephrosis , indicating severe podocyte injury . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The relationship among nephrin , podocin , CD2AP , and alpha actinin might not be a true ' interaction ' in podocyte . ^^^ We respectively knockdown the nephrin , podocin , CD2AP , or alpha actinin 4 mRNA by using reconstructed RNA interference vector psiRNA hH1GFPzeo in mouse podocyte clone . ^^^ With nephrin knockdown , only CD2AP increased , whereas podocin showed no change . ^^^ Contrarily , with podocin or CD2AP knockdown , nephrin decreased , while CD2AP or podocin increased . ^^^ Nephrin , podocin , or CD2AP knockdown did not change the expression of alpha actinin 4 , whereas alpha actinin 4 knockdown begetted the reduction of nephrin , and the increment of podocin and CD2AP . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| On the cellular level using immunostaining we detected cells expressing podocin , nephrin and wt 1 , characteristic for differentiated podocytes and other cells , which expressed Tamm Horsfall protein , a marker for distal tubule epithelial cells of kidney tissue . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Sema 3A induced a dose response podocin downregulation and decreased its interaction with CD 2 associated protein and nephrin , as determined by Western analysis and co immunoprecipitation . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| No significant reduction of slit membrane molecules ( podocin and nephrin ) , key GBM components ( fibronectin , laminins , and collagen 4 isoforms ) , or podocyte integrins could be observed at onset of proteinuria . ^^^ |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Nephrin , podocin , CD2AP , and alpha actinin 4 are important podocyte proteins that help maintain the integrity of the slit diaphragm and prevent proteinuria . ^^^ In this study , changes in the expression and distribution of nephrin , podocin , CD2AP , and alpha actinin 4 were dynamically detected in Adriamycin induced nephrotic ( ADR ) rats treated with three different drugs : lisinopril , prednisone , and ATRA . ^^^ The distribution and the expression of messenger RNA and protein of nephrin , podocin , CD2AP , and alpha actinin 4 were detected by indirect immunofluorescence , real time polymerase chain reaction , and Western blotting , respectively . ^^^ With the intervention of lisinopril , prednisone , and ATRA , changes in the expression of nephrin , podocin , and CD2AP were diverse , which was different from that detected in ADR rats . ^^^ After lisinopril and prednisone intervention , podocin exhibited prominent earlier changes compared with those of nephrin and CD2AP , whereas CD2AP showed more prominent changes after ATRA intervention . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Proteins encoded by these genes ( nephrin , podocin , alpha actinin 4 , an adapter protein anchoring CD 2 and others ) influence the function of the podocytes . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Nephrin , Neph 1 and podocin seem to form a multifunctional receptor complex at the slit diaphragm . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Familial forms of focal segmental glomerulosclerosis ( FSGS ) are caused by mutations in genes at 1q25 31 ( gene for steroid resistant nephrotic syndrome 2 [ NPHS 2 ] ) , 11q21 22 , 19q13 ( gene for alpha actinin 4 and NPHS 1 ) , and at additional unidentified chromosomal loci . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Heterozygous NPHS 1 or NPHS 2 mutations in responsive nephrotic syndrome and the multifactorial origin of proteinuria . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The candidate genes were selected based on their functions , including insulin resistance ( APM 1 , CD 36 , HSD11B1 ) , oxidative stress ( CYBA , GPX 1 , GSTMs ) , steroid hormone ( ESR 1 , ESR 2 , HSD11B2 ) , renal functions ( PTGS 2 , KLK 1 , NPHS 1 , NPHS 2 , SGK , SLC12A1 , PTGES ) , and others related to cardiovascular physiology ( GJA 4 , NOS 1 , NTRK 3 , P2RX4 , SPP 1 , ALDH 2 ) . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Congenital nephrotic syndrome ( CNS ) is caused by mutations in NPHS 1 ( nephrin ) or NPHS 2 . ^^^ No evidence for genotype / phenotype correlation in NPHS 1 and NPHS 2 mutations . ^^^ In three families mutations in NPHS 1 and NPHS 2 had been reported to occur together , and these tri allelic mutations were implicated in genotype / phenotype correlations . ^^^ To further test the hypothesis of tri allelism , we examined a group of 62 unrelated patients for NPHS 1 mutations , who were previously shown to have NPHS 2 mutations ; 15 of 62 patients had CNS . ^^^ In addition , 12 CNS patients without NPHS 2 mutation were examined for NPHS 1 mutations . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Congenital nephrotic syndrome ( CNS ) is clinically and genetically heterogeneous , with mutations in WT 1 , NPHS 1 and NPHS 2 accounting for part of cases . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Mutations of NPHS 1 , NPHS 2 , or WT 1 may be responsible for severe forms of nephrotic syndrome in children , progressing to end stage renal failure . ^^^ Recent studies have shown that congenital nephrotic syndrome may be secondary to mutations of one of these three genes and that some patients have a digenic inheritance of NPHS 1 and NPHS 2 mutations . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Therefore , to investigate the mechanism of Wt 1 R394W induced renal failure , the expression of genes whose deletion leads to glomerulosclerosis ( NPHS 1 , NPHS 2 , and CD2AP ) was quantitated . ^^^ In mutant kidneys , NPHS 1 and NPHS 2 were only moderately downregulated ( 25 to 30 % ) at birth but not at 2 or 4 months . ^^^ However , the data do suggest that Wt 1 R394W induced glomerulosclerosis may be independent of downregulation of the genes for NPHS 1 , NPHS 2 , CD2AP , and podocalyxin and may involve other genes yet to be implicated in renal failure . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| TERalb ( % / h ) was similar in normoalbuminuric and microalbuminuric group 1 , 2 , and 3 diabetic patients ( medians : 14 . 1 vs . 14 . 4 vs . 15 . 7 vs . 14 . 9 , respectively ) ( ANOVA , NS ) . mRNA expression of slit diaphragm proteins CD2AP , FAT , Actn 4 , NPHS 1 , and NPHS 2 was higher in normoalbuminuric patients than in microalbuminuric patients ( groups 1 , 2 , and 3 ) ( ANOVA , P < 0 . 001 ) . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Associations with various genes ( NPHS 1 , ACTN 4 , NPHS 2 , WT 1 ) and linkage to several chromosomal regions ( such as 19q13 , 11q21 , 11q24 ) have been reported in patients with familial NS / FSGS . . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Reported here is a case of heterozygous NPHS 1 mutation , with normal NPHS 2 gene structure , presenting during prenatal screening and developing nephrotic syndrome within days of birth . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| Real time polymerase chain reaction measurement of mRNA SD proteins ( CD2AP , FAT , Actn 4 , NPHS 1 , and NPHS 2 ) significantly increased in kidney biopsy specimens after simvastatin , but not cholestyramine treatment . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| The majority of cases can be attributed to mutations in the genes NPHS 1 , NPHS 2 , and WT 1 . ^^^ We conclude that mutational analysis in LAMB 2 should be considered in congenital nephrotic syndrome , if no mutations are found in NPHS 1 , NPHS 2 , or WT1 . . ^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.54096402 |
| Moreover , GST pull down experiments reveal that podocin associates via its COOH terminal domain with CD2AP , a cytoplasmic binding partner of nephrin , and with nephrin itself . 0.54096402^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.68887857 |
| In addition , nephrin was found to partially colocalize with CD2AP and podocin in double immunofluorescence microscopy , confirming the close proximity of these proteins and proposing that these proteins may belong to nephrin associated protein complex in glomeruli . 0.68887857^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.56329473 |
| There was close relationship between nephrin , podocin protein level and distribution pattern with the development of proteinuria and podocyte foot process effacement , whereas no major role was found for mRNA of nephrin and podocin . 0.56329473^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.61532142 |
| These results suggest that podocin may interact directly with nephrin , but not with alpha actinin . . 0.61532142^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.91861521 |
| In contrast , podocin and CD2AP , two proteins shown to interact with nephrin in the slit diaphragm , are acutely downregulated at days 3 7 and , thereafter , recovered again to normal levels after 29 days . 0.91861521^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.55316654 |
| It is reported that podocin interacts with nephrin and CD2AP , and that NEPH 1 interacts with nephrin . 0.55316654^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.68341569 |
| The intracellular domain of nephrin is known to interact with another slit diaphragm protein , podocin . 0.68341569^^^ |
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| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9NP85 and O60500 |
Pubmed |
SVM Score :0.0 |
| NA |
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