| Interacting proteins: O60500 and P16333 |
Pubmed |
SVM Score :0.50985095 |
| Nephrin from normal rat glomeruli was also tyrosine phosphorylated and associated with Nck . 0.50985095^^^ |
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| Interacting proteins: O60500 and P16333 |
Pubmed |
SVM Score :0.0 |
| Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes . ^^^ Here we show that nephrin selectively binds the Src homology 2 ( SH 2 ) / SH3 domain containing Nck adaptor proteins , which in turn control the podocyte cytoskeleton in vivo . ^^^ The cytoplasmic tail of nephrin has multiple YDxV sites that form preferred binding motifs for the Nck SH 2 domain once phosphorylated by Src family kinases . ^^^ We show that this Nck nephrin interaction is required for nephrin dependent actin reorganization . ^^^ Together , these findings identify a physiological signalling pathway in which nephrin is linked through phosphotyrosine based interactions to Nck adaptors , and thus to the underlying actin cytoskeleton in podocytes . ^^^ |
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| Interacting proteins: O60500 and P16333 |
Pubmed |
SVM Score :0.0 |
| Nephrin ectodomain engagement results in Src kinase activation , nephrin phosphorylation , Nck recruitment , and actin polymerization . ^^^ We found that this nephrin phosphorylation event resulted in recruitment of the SH 2 SH3 domain containing adapter protein Nck and assembly of actin filaments in an Nck dependent fashion . ^^^ |
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| Interacting proteins: O60500 and P16333 |
Pubmed |
SVM Score :0.0 |
| Nck links nephrin to actin in kidney podocytes . ^^^ Two papers , one in Nature ( Jones et al . , 2006 ) and the other in the Journal of Clinical Investigation ( Verma et al . , 2006 ) show that Nck adaptor proteins connect phosphorylated nephrin with actin polymerization in podocyte foot processes , structures important for slit diaphragm formation in the kidney . ^^^ |
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