| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.7836926 |
| VEGF ( 145 ) binds to np 2 with high affinity ( K ( D ) 7 10 10 ( 10 ) m ) . 0.7836926^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.5641388 |
| NRP 2 specifically binds VEGF A and VEGF C , although the biological relevance of these interactions in human endothelial cells is poorly understood . 0.5641388^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Endothelial cells express in addition the neuropilin 1 and neuropilin 2 coreceptors , which bind selectively to the 165 amino acid form of VEGF ( VEGF 165 ) . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| These receptors are encoded by the neuropilin 1 gene , and we have recently found that the related neuropilin 2 gene also encodes a VEGF 165 receptor . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Using PCR analysis , we also identified partial sequences of multiple VEGF isoforms ( VEGF 188 , 183 , 164 , and 120 ) as well as VEGFR 1 , VEGFR 2 , and neuropilin 2 ( NP 2 ) from the OFPAE cell cDNA library . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| The neuropilin 1 ( NRP 1 ) and neuropilin 2 ( NRP 2 ) receptors can bind the class 3 semaphorin subfamily and the heparin binding forms of vascular endothelial growth factor ( VEGF ) and placenta growth factor ( PlGF ) . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| The interaction of Neuropilin 1 and Neuropilin 2 with tyrosine kinase receptors for VEGF . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Reverse transcriptase polymerase chain reaction ( RT PCR ) is utilized to measure the VEGF receptors flt 1 , KDR / flk 1 , flt 4 , neuropilin 1 ( NRP 1 ) , and neuropilin 2 ( NRP 2 ) . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Stimulation by either 50 ng / ml VEGF A 165 or by 24 h of anoxia resulted in an altered pattern of receptor expression for VEGF R 2 ( Flk 1 ) , VEGF R 3 ( Flt 4 ) , neuropilin 1 , and neuropilin 2 , whereas VEGF R 1 ( Flt 1 ) remained unexpressed . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Neuropilin 1 ( NP 1 ) and neuropilin 2 ( NP 2 ) are receptors for semaphorins , which act as axonal chemorepellents , and for members of the vascular endothelial growth factor ( VEGF ) family of angiogenic growth factors . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| In addition , receptors for the secreted semaphorins , neuropilin 1 and neuropilin 2 , have been shown to function as coreceptors for a subset of vascular endothelial growth factor ( VEGF ) isoforms and related growth factors . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Neuropilin 1 ( NRP 1 ) and neuropilin 2 ( NRP 2 ) are both receptors for semaphorins , which regulate neuronal guidance , and vascular endothelial growth factor ( VEGF ) , an angiogenic factor . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Neuropilin 1 ( np 1 ) and neuropilin 2 ( np 2 ) are receptors for class 3 semaphorins and for several isoforms of VEGF . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Neuropilin 2 ( NP 2 ) is a cell surface transmembrane protein originally characterized as a receptor for the type 3 semaphorins , and more recently for a number of vascular endothelial growth factor ( VEGF ) isoforms . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Message for VEGF and the VEGF receptors KDR , flt 1 , flt 4 , neuropilin 1 ( NRP 1 ) , and neuropilin 2 ( NRP 2 ) are measured with RT PCR . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| The results showed that a large number of hematopoietic growth factor and receptor genes ( Epo / R , GM CSF / R , G CSF / R , LIF , IL 3 , TPO , Flt 3 , SCF ) were expressed in both groups , while many other growth factors ( VEGF , IGF 2 , PDGFA , PDGFB , TGFbeta 1 ) and receptors ( neuropilin 1 , neuropilin 2 , TGFbeta R 1 ) were expressed . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Deficiency of neuropilin 2 suppresses VEGF induced retinal neovascularization . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Neuropilin 1 ( NRP 1 ) and neuropilin 2 ( NRP 2 ) are transmembrane glycoproteins that have been characterized as receptors for both semaphorins for neuronal guidance and vascular endothelial growth factor ( VEGF ) for angiogenesis . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| The neuropilin 1 ( np 1 ) and the neuropilin 2 ( np 2 ) receptors bind vascular endothelial growth factor ( VEGF ) and class 3 semaphorins . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Gene targeting experiments indicate that np 1 does indeed function as an important modulator of VEGF function during vasculogenesis and angiogenesis , but the role of np 2 in the cardiovascular system has not been studied in detail as yet . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Neuropilins ( NP 1 and NP 2 ) are vascular endothelial growth factor ( VEGF ) receptors that mediate developmental and tumor angiogenesis . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| To examine whether semaphorins could modulate VEGF endothelial cell guidance cues in the developing kidney , we studied the expression of semaphorin 3A and semaphorin 3F and their receptors NP 1 and NP 2 in the kidney during ontogeny using Northern blot analysis , in situ hybridization , Western blot analysis and immunohistochemistry . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Expression of VEGF , semaphorin SEMA3F , and their common receptors neuropilins NP 1 and NP 2 in preinvasive bronchial lesions , lung tumours , and cell lines . ^^^ Elevated VEGF levels are associated with the loss and cytoplasmic delocalization of SEMA3F in lung cancer , suggesting competition for their NP 1 and NP 2 receptors . ^^^ To determine the timing of these events , we compared by immunohistochemistry VEGF , SEMA3F , NP 1 and NP 2 expression in 50 preneoplastic lesions and 112 lung tumours . ^^^ NP 1 and NP 2 levels increased from dysplasia to microinvasive carcinoma ( p=0 . 0001 ) and correlated with VEGF expression ( p=0 . 04 and 0 . 0002 , respectively ) . ^^^ Non small cell lung carcinoma overexpressed VEGF and NP 1 and NP 2 significantly more often than neuroendocrine tumours including small cell lung carcinoma . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NP 1 and NP 2 are also coreceptors for vascular endothelial growth factor ( VEGF ) . ^^^ Neurol . 172 ( 2001 ) 398 ) that VEGF mRNA levels rise during Wallerian degeneration in the PNS and herein demonstrate that NP 1 , NP 2 , Sema3A , and Sema3F mRNA levels increase in peripheral nerves distal to a transection or crush injury . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| MATERIALS AND METHODS : Using RT PCR we examined the expression of VEGF and its receptors , KDR , Flt 1 , NP 1 and NP 2 , in 8 human prostate cancer cell lines and then correlated the expression patterns with the ability of these cell lines to invade and migrate through membranes in a modified Boyden Chamber assay in the presence and absence of VEGF . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Np 1 and np 2 function in addition as receptors for heparin binding forms of vascular endothelial growth factor ( VEGF ) , such as VEGF ( 165 ) . ^^^ Human umbilical vein endothelial cells ( HUVEC ) express tyrosine kinase receptors for VEGF and basic fibroblast growth factor ( bFGF ) , as well as np 1 , np 2 , and several type A plexins . ^^^ We have found that semaphorin 3F ( s3f ) , a semaphorin which signals through the np 2 receptor , was able to inhibit VEGF ( 165 ) , as well as bFGF induced proliferation of HUVECs . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| By contrast , VEGF 121 , a VEGF variant that lacks binding to neuropilin ( NP ) 1 or NP 2 receptors , was not expressed in tumor cells and had no effect on SEMA3B growth suppressing activities . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Although much is known about the biology of vascular endothelial growth factor ( VEGF ) and its cognate receptors ( VEGFRs ) , VEGFR 1 and VEGFR 2 , little is known about the roles of the VEGFRs neuropilin ( NP ) 1 and NP 2 in the primate endometrium . ^^^ The increased permeability and edema observed during the secretory phase in the primate endometrium may be mediated in part by VEGF NP 2 interaction . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Here , we examined the expression of VEGF A and its receptors VEGFR 1 , VEGFR 2 , NP 1 , and NP 2 in conditionally immortalized mouse podocytes cultured in undifferentiated and differentiated conditions using RT PCR and Western analysis . ^^^ VEGF ( 165 ) induced a twofold increase in VEGFR 2 mRNA and protein levels , whereas VEGFR 1 , sVEGFR 1 , NP 1 , and NP 2 mRNA levels remained unchanged . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Retinal mRNA expression of VEGF splice variants ( VEGF 188 , VEGF 164 , VEGF 120 ) , VEGF receptors ( VEGFR 1 , VEGFR 2 , Npn 1 , Npn 2 ) , and pigment epithelium derived factor ( PEDF ) were also examined . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NRP 2 expression cooperatively promotes angiogenesis and growth in osteosarcomas with VEGF expression . . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| In the present study , we first show that VEGF selectively up regulates NRP 1 but not NRP 2 via the VEGF receptor 2 dependent pathway . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| The mRNA expression of VEGF ( 165 ) and its receptors Flt 1 , KDR , NRP 1 , and NRP 2 was evaluated by real time reverse transcription polymerase chain reaction . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| EXPERIMENTAL DESIGN : We assessed Np 1 and Np 2 expression by real time quantitative PCR in relation to the expression of VEGF ligands and receptors in pancreatic cancer cell lines and tissues . ^^^ RESULTS : ASPC 1 , CAPAN 1 , and PANC 1 pancreatic cancer cells and tumor derived , laser captured pancreatic cancer cells exhibited higher Np 1 and Np 2 mRNA levels than VEGF receptor 1 , 2 , or 3 mRNA levels . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| These results suggest that both NRP 1 and NRP 2 are key molecules for stromal vascularization by cell retained VEGF in NSCLC . . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| In vascular endothelial cells , NRP 1 functions as an isoform specific receptor for VEGF 165 and has predominant expression compared to NRP 2 . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| We examined the gene expression of vascular endothelial growth factor ( VEGF ) A , Flt 1 , KDR , NRP 1 and NRP 2 in 37 gliomas by real time reverse transcriptase PCR ( real time RT PCR ) as well as immunohistochemical analysis . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Here , we observed in vitro that new ( 99m ) Tc labeled derivative of anti VEGF heptapeptide , ATWLPPR , binds to NRP 1 but not to NRP 2 . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : These data demonstrate that HUVECs express higher mRNA levels of NRP 1 and NRP 2 than those of VEGFRs , and TNF alpha treatment significantly decreases the expression of VEGFR 2 , VEGFR 3 , and NRP 1 , which may be responsible for TNF alpha induced reduction of EC proliferation in response to VEGF ( 165 ) . . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Although mRNA for VEGF 121 , VEGF 165 , VEGFR 1 and NRP 2 was widely expressed , mRNA expression for VEGF 189 , VEGFR 2 and NRP 1 was linked to a malignant phenotype such as local invasion . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Although both antagonize VEGF binding / response to neuropilin ( NRP ) receptors , in lung cancer lines , SEMA3F is predominantly expressed and preferentially utilizes NRP 2 . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Both types of NRP , NRP 1 and NRP 2 , which only bind VEGF 164 , increased their expression about 2 folds only at P 30 , at levels similar to those observed for VEGF164 . . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| Although much is known about the biology of vascular endothelial growth factor ( VEGF ) and its receptors , little is known about the role of the VEGF receptors neuropilin ( NRP ) 1 and NRP 2 in the process of bovine follicle development . ^^^ |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O60462 and P15692 |
Pubmed |
SVM Score :0.0 |
| NA |
|