| Interacting proteins: O60313 and P14672 |
Pubmed |
SVM Score :0.0 |
| Rates of glucose transport and metabolism were assessed by hind limb perfusion in GLUT 4 transgenic ( TG ) mice and non transgenic ( NTG ) controls . ^^^ Membrane purification revealed that , under basal conditions , plasma membrane ( approximately 12 fold ) and intracellular membrane ( approximately 4 fold ) GLUT 4 protein concentrations were greater in TG than NTG mice . ^^^ Submaximal insulin stimulation did not increase plasma membrane GLUT 4 protein concentration whereas maximal insulin stimulation increased this protein in both NTG ( 4 . 1 fold ) and TG ( 2 . 6 fold ) mice . ^^^ |
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| Interacting proteins: O60313 and P14672 |
Pubmed |
SVM Score :0.0 |
| Thus we have assessed cell surface GLUT 4 by photolabelling with the membrane impermeant reagent 2 N [ 4 ( 1 azi 2 , 2 , 2 trifluoroethyl ) benzoyl ] 1 , 3 bis ( D mannos 4 yloxy ) 2 propylamine ( ATB BMPA ) and measured the corresponding glucose transport activity using 2 deoxyglucose in isolated extensor digitorum longus ( EDL ) muscles from non transgenic ( NTG ) and GLUT 4 TG mice in the absence and presence of 13 . 3 nM ( 2000 mu units / ml ) insulin , without or with hypoxia as a model of muscle contraction . ^^^ Subcellular fractionation of hindlimb muscles confirmed the previously observed 3 fold overexpression of GLUT 4 in the TG compared with the NTG mice . ^^^ These results suggest that : ( 1 ) alterations in glucose transport activity which occur with GLUT 4 overexpression in EDL muscles are directly related to cell surface GLUT 4 content , regardless of the levels observed in the corresponding subcellular membrane fractions , ( 2 ) while overexpression of GLUT 4 influences both basal and insulin stimulated glucose transport activity , the response to hypoxia / contraction stimulated glucose transport is unchanged , and ( 3 ) subcellular fractionation provides little insight into the subcellular trafficking of GLUT 4 , and whatever relationship is demonstrated in EDL muscles from NTG mice is disrupted on GLUT 4 overexpression . . ^^^ |
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| Interacting proteins: O60313 and P14672 |
Pubmed |
SVM Score :0.0 |
| In young mice ( age 10 12 weeks ) , both lower ( two to threefold ) and higher ( four to fivefold ) levels of GLUT 4 overexpression were associated with improved glucose tolerance compared to age matched nontransgenic ( NTG ) mice . ^^^ Glucose infusion rates during hyperinsulinemic hyperglycemic clamps were increased with GLUT 4 overexpression , compared with NTG mice in both lower and higher levels of GLUT 4 overexpression , even in the older mice . ^^^ |
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| Interacting proteins: O60313 and P14672 |
Pubmed |
SVM Score :0.0 |
| Similarly , GLUT 4 translocation was significantly reduced in NTG ( 74 + / 7 % ) and 8 Br cGMP ( 120 + / 11 % ) , compared with control ( 165 + / 17 % ) . ^^^ |
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