| Syntaxin 6 regulates Glut 4 trafficking in 3T3 L 1 adipocytes . ^^^ Greater than 85 % of syntaxin 6 was found in Glut 4 containing vesicles , and this t SNARE exhibited insulin stimulated movement to the plasma membrane . ^^^ Overexpression of the cytosolic domain of syntaxin 6 did not affect insulin stimulated glucose transport , but increased basal deGlc transport and cell surface Glut 4 levels . ^^^ Moreover , the syntaxin 6 cytosolic domain significantly reduced the rate of Glut 4 reinternalization after insulin withdrawal and perturbed subendosomal Glut 4 sorting ; the corresponding domains of syntaxins 8 and 12 were without effect . ^^^ Our data suggest that syntaxin 6 is involved in a membrane trafficking step that sequesters Glut 4 away from traffic destined for the plasma membrane . ^^^ |