| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.6721471 |
| In addition , by co immunoprecipitation , we showed that intact Hprp3p and its mutants containing the central region interacted with Hprp4p in HeLa cell nuclear extracts . 0.6721471^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Mapping of the human HPRP 3 and HPRP 4 genes encoding U4 / U6 associated splicing factors to chromosomes 1q21 . 1 and 9q31 q 33 . ^^^ |
|
| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Identification and characterization of human genes encoding Hprp3p and Hprp4p , interacting components of the spliceosome . ^^^ We report here the identification and characterization of two human genes encoding U4 / U6 associated splicing factors , Hprp3p and Hprp4p , respectively . ^^^ Hprp3p and Hprp4p have been shown to interact with each other and the first 100 amino acids of Hprp3p are not essential for this interaction . ^^^ These experiments suggest that both Hprp3p and Hprp4p are components of human spliceosomes . . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| While PRP 38 lacks obvious structural similarity to previously defined splicing factors , heat inactivation of PRP 38 , PRP 19 , or any of the known U 6 ( or U4 / U6 ) small nuclear ribonucleoprotein associating proteins ( i . e . , PRP 3 , PRP 4 , PRP 6 , and PRP 24 ) leads to a common , unexpected consequence : intracellular U 6 small nuclear RNA ( snRNA ) levels decrease as splicing activity is lost . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Somewhat higher concentrations of PRP 3 and PRP 4 were required for maximal adsorption , indicating that the 44 residue carboxy terminal segment of PRP 1 , PRP 2 , and PIF slow enhances LY 7 binding but is not essential . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| These are PRP 1 , also designated Protein C , PRP 3 , also designated Protein A ( 17 ) , and PRP 4 . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Neither spp 41 nor spp 42 suppressors null alleles of prp 3 or prp 4 , indicating that the suppression does not occur via a bypass mechanism . ^^^ Expression of PRP 3 lacZ and PRP 4 lacZ gene fusions is increased in spp 41 strains , suggesting that wild type Spp41p represses expression of PRP 3 and PRP 4 . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| The effects on prp 4 mutant growth of increased copy number of mutant prp 4 genes themselves , and of genes for other components of the U4 / U6 snRNP ( PRP 3 and U 6 snRNA ) have also been studied . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| A faster adsorption process is observed for PRP 3 , PRP 4 , PIF f , and statherin . ^^^ |
|
| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| A new cyclophilin and the human homologues of yeast Prp 3 and Prp 4 form a complex associated with U4 / U6 snRNPs . ^^^ Two of the proteins are homologues of the Saccharomyces cerevisiae splicing factors Prp 3 and Prp 4 , and the third protein is a cyclophilin . ^^^ The human homologues of Prp 3 and Prp 4 are part of a stable complex in the absence of RNA . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Two are allelic with the previously described prp 3 and prp 4 mutations ; the remaining four define the new alleles prp 5 1 , prp 6 1 , prp 7 1 , and prp 9 1 . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Functional and structural characterization of the prp 3 binding domain of the yeast prp 4 splicing factor . ^^^ |
|
| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Thus the potential for human leucocyte elastase mediated removal of the negatively charged amino terminal region of acidic PRP variants ( PRP 1 , PRP 2 , PRP 3 , PRP 4 , PIF s and PIF f ) was examined . ^^^ |
|
| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| It stably associates with the U4 / U6 60kD and 90kD proteins , the human orthologues of the Saccharomyces cerevisiae Prp 4 and Prp 3 splicing factors . ^^^ |
|
| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Prp 3 , Prp 4 and Prp 18 proteins exist in S . pombe and nuclear localisation of SpCyp 3 has been shown , indicating conservation of function between hUSA CyP and SpCyp3 . . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Apart from Pa , all the other aPRPs undergo a proteolytic cleavage at Arg 106 residue ( Arg 127 in Db s protein ) , that generates the small PC peptide ( 4371 amu ) and PRP 3 , PRP 4 , PIF f ( 11 , 162 amu ) and Db f ( 13 , 280 amu ) proteins , all of which were detected . ^^^ Besides these known isoforms , several previously undetected aPRP derivatives were found ( in minor amounts ) : ( 1 ) the triphosphorylated derivatives of PRP 1 / PRP 2 / PIF s and Db s , showing the additional phosphate group at Ser 17 ; ( 2 ) the mono phosphorylated forms at either Ser 22 or Ser 8 of PRP 1 / PRP 2 / PIF s , PRP 3 / PRP 4 / PIF f , Db s and Db f ; ( 3 ) a nonphosphorylated form of PRP 3 / PRP 4 / PIF f ; ( 4 ) the triphosphorylated and diphosphorylated forms of Pa 2 mer . ^^^ Moreover , minor quantities of PRP 3 / PRP 4 / PIF f lacking the C terminal Arg ( 11 , 006 amu ) , and of Pa 2 mer lacking the C terminal Gln ( 30 , 793 amu ) were found . ^^^ |
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| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| The human U4 / U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p . ^^^ |
|
| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| Mutations in the U4 / U6 snRNP components Prp3p , Prp4p , and Prp24p led to disassembly of the U4 / U6 snRNP particle and degradation of the U 6 snRNA , while prp 17 1 and prp 19 1 strains accumulated free U 4 and U 6 snRNA . ^^^ |
|
| Interacting proteins: O43395 and O43172 |
Pubmed |
SVM Score :0.0 |
| NA |
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