Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.53469985
In vivo , MOP 3 interacts with CLOCK to regulate circadian rhythms ; however , its role in hypoxia responses is unclear . 0.53469985^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We demonstrated that MOP 3 interacts with MOP 4 , CLOCK , hypoxia inducible factor 1alpha ( HIF1alpha ) , and HIF2alpha . ^^^ Transient transfection experiments demonstrated that the MOP 3 MOP4 and MOP 3 CLOCK complexes bound this element in COS 1 cells and drove transcription from a linked luciferase reporter gene . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Potential partners of CLOCK were isolated in a two hybrid screen , and one , BMAL 1 , was coexpressed with CLOCK and PER 1 at known circadian clock sites in brain and retina . ^^^ CLOCK BMAL 1 heterodimers activated transcription from E box elements , a type of transcription factor binding site , found adjacent to the mouse per 1 gene and from an identical E box known to be important for per gene expression in Drosophila . ^^^ Mutant CLOCK from the dominant negative Clock allele and BMAL 1 formed heterodimers that bound DNA but failed to activate transcription . ^^^ Thus , CLOCK BMAL 1 heterodimers appear to drive the positive component of per transcriptional oscillations , which are thought to underlie circadian rhythmicity . . ^^^ Here , the Drosophila CLOCK protein was shown to induce transcription of the circadian rhythm genes period and timeless . dCLOCK functioned as a heterodimer with a Drosophila homolog of BMAL 1 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Circadian oscillation of BMAL 1 , a partner of a mammalian clock gene Clock , in rat suprachiasmatic nucleus . ^^^ A robust circadian rhythm of rat BMAL 1 expression was detected by in situ hybridization in the suprachiasmatic nucleus ( SCN ) , the site of the circadian clock , with the highest level at the subjective night . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Over the past year , the first components of the mammalian clock have been identified ; Clock , bmal 1 and three homologs of Drosophila period have been cloned , all of which encode PAS proteins . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Circadian rhythm and light responsiveness of BMAL 1 expression , a partner of mammalian clock gene Clock , in the suprachiasmatic nucleus of rats . ^^^ To clarify whether BMAL 1 is involved in the photic signal transduction in the mammalian circadian clock , we examined the effects of a single light pulse on the level of BMAL 1 mRNA in the suprachiasmatic nucleus ( SCN ) of rats by in situ hybridization . ^^^ These results indicate that BMAL 1 transcription is not involved in the photic signal transduction responsible for non parametric entrainment of the circadian clock in rats . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
A core clock mechanism in mouse SCN appears to involve a transcriptional feedback loop in which CLOCK and BMAL 1 are positive regulators and three mPeriod ( mPer ) genes are involved in negative feedback . ^^^ Luciferase reporter gene assays show that CLOCK BMAL 1 heterodimers act through an E box enhancer in the vasopressin gene to activate transcription ; this activation can be inhibited by the mPER and mTIM proteins . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Previously we described the functional identification and molecular isolation of the Clock gene in the mouse , its interaction with the BMAL 1 protein , and the role of this complex as a transcriptional activator in the circadian pacemaker . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Mammalian CRY 1 and CRY 2 are here shown to act as light independent inhibitors of CLOCK BMAL 1 , the activator driving Per 1 transcription . ^^^ CRY 1 or CRY 2 ( or both ) showed light independent interactions with CLOCK and BMAL 1 , as well as with PER 1 , PER 2 , and TIM . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Daily variation and light responsiveness of mammalian clock gene , Clock and BMAL 1 , transcripts in the pineal body and different areas of brain in rats . ^^^ Expression patterns of mammalian clock genes , Clock and BMAL 1 , were examined by in situ hybridization in the pineal body , olfactory bulb , hippocampus and cerebellum in rats under constant darkness . ^^^ In the pineal , the level of Clock transcript was significantly higher at ZT 18 ( subjective night ) than at ZT 6 ( subjective day ) , while the level of BMAL 1 transcript was significantly higher at ZT 6 than at ZT 18 . ^^^ A 30 min light pulse did not affect the transcript levels of Clock nor of BMAL 1 . ^^^ These findings indicate that the mammalian clock gene , Clock and BMAL 1 , are expressed differently in the different areas of the brain and the pineal . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Key molecular components such as Clock , Bmal 1 , Timeless and three Period proteins have been isolated in mammals . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In cell lines , mPER proteins ( alone or in combination ) have modest effects on their cellular location and ability to inhibit CLOCK : BMAL 1 mediated transcription . ^^^ Luciferase reporter gene assays show that mCRY 1 or mCRY 2 alone abrogates CLOCK : BMAL 1 E box mediated transcription . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Circadian rhythms and light responsiveness of mammalian clock gene , Clock and BMAL 1 , transcripts in the rat retina . ^^^ Circadian expression and light responsiveness of the mammalian clock genes , Clock and BMAL 1 , in the rat retina were examined by in situ hydbribization under constant darkness . ^^^ A small but significant daily variation was detected in the Clock transcript level , but not in BMAL 1 . ^^^ Light increased the Clock and BMAL 1 expressions significantly when examined 60 min after exposure . ^^^ The light induced gene expression was phase dependent for Clock and peaked at ZT 2 , while rather constant throughout the day for BMAL 1 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Northern blot analysis indicated that several putative clock genes ( bmal 1 , Clock , and MOP 4 ) are co expressed in the chicken pineal gland . bmal 1 mRNA is expressed in a rhythmic manner in the chicken pineal gland , with peak levels at early subjective night , coincident with the increase in cAANAT expression . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The basic helix loop helix PAS proteins CLOCK and BMAL 1 are positive regulators and drive the expression of the negative regulators CRY 1 and CRY 2 , as well as PER 1 , PER 2 , and PER 3 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Rhythmic expression of BMAL 1 mRNA is altered in Clock mutant mice : differential regulation in the suprachiasmatic nucleus and peripheral tissues . ^^^ BMAL 1 is a putative clock gene which encodes a basic helix loop helix ( bHLH ) PAS transcription factor . ^^^ To examine whether the CLOCK protein is required for the circadian expression of BMAL 1 mRNA , in situ hybridization and Northern blot analysis were performed in the suprachiasmatic nucleus ( SCN ) and peripheral tissues of homozygous Clock mutant mice . ^^^ In the SCN of Clock mutants , BMAL 1 mRNA did not oscillate significantly but apparently expressed with low levels , while in wild type mice the mRNA was robustly oscillated in a circadian manner . ^^^ Furthermore , expressions of BMAL 1 mRNA in the peripheral of Clock mutant mice were close to the peak level in wild type mice , whereas mPer 2 mRNA levels were severely blunted at trough values . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Using a two hybrid system approach , we have isolated two different partners of zebrafish ( zf ) CLOCK , which are similar to the mammalian BMAL 1 ( brain and muscle arylhydrocarbon receptor nuclear translocator like protein 1 ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Analysis of Clock / Clock mutant mice , homozygous Period 2 ( Brdm 1 ) mutants , and Cryptochrome deficient mice reveals substantially altered Bmal 1 rhythms , consistent with a dominant role of PERIOD 2 in the positive regulation of the Bmal 1 loop . ^^^ In vitro analysis of CRYPTOCHROME inhibition of CLOCK : BMAL 1 mediated transcription shows that the inhibition is through direct protein : protein interactions , independent of the PERIOD and TIMELESS proteins . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
A conserved set of genes in Drosophila and mammals ( Clock , Bmal 1 , Period , and Timeless ) provide a molecular framework for the circadian mechanism . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The per gene is activated by a constitutively expressed heterodimeric protein encoded by the bmal 1 and clock genes , and this activation is suppressed by the PER protein itself . ^^^ These elements of biomolecular feedback loops are interpreted within a system theory as an elementary behavioral cycle consisting of intentional programs ( the per gene ) , environmental objects ( the BMAL 1 CLOCK heterodimer ) and the experiential realization of the intended programs ( the level of PER protein ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
One has a high sequence similarity to mammalian cry genes and inhibits CLOCK : BMAL 1 mediated transcription . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
BACKGROUND : The Arnt 3 ( also termed as BMAL 1 or MOP 3 ) / Clock heterodimer is a positive regulator of circadian rhythm and activates the transcription of target genes such as per 1 and vasopressin . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Light and glutamate induced degradation of the circadian oscillating protein BMAL 1 during the mammalian clock resetting . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Two basic helix loop helix ( bHLH ) PAS ( PER ARNT SIM ) transcription factors , CLOCK and BMAL 1 , form the positive elements of the system and drive transcription of three Period and two Cryptochrome genes . ^^^ The protein products of these genes are components of a negative feedback complex that inhibits CLOCK and BMAL 1 to close the circadian loop . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Many of these insights have shown or foreshadow direct parallels in mammalian systems , including the mechanism of light entrainment , the involvement of PAS : PAS heterodimers as transcriptional activators in essential clock associated feedback loops , and dual role of FRQ in the loop as an activator and a repressor ; similarities extend to the primary sequence level in at least one case , that of WC 1 and BMAL 1 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
A core clock mechanism in the mouse SCN appears to involve a transcriptional feedback loop in which CLOCK and BMAL 1 function as positive regulators and three mPeriod ( mPer ) genes play a role in negative feedback . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
CLOCK protein and BMAL 1 protein , first discovered as components of the circadian clock in mammals , are known to function as transcriptional activators in the circadian feedback loop of drosophila . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Here , using wild type and cryptochrome ( mCry ) deficient cell lines derived from mCry mutant mice , we show that the peripheral oscillator in cultured fibroblasts is identical to the oscillator in the SCN in ( 1 ) temporal expression profiles of all known clock genes , ( 2 ) the phase of the various mRNA rhythms ( i . e . , antiphase oscillation of Bmal 1 and mPer genes ) , ( 3 ) the delay between maximum mRNA levels and appearance of nuclear mPER 1 and mPER 2 protein , ( 4 ) the inability to produce oscillations in the absence of functional mCry genes , and ( 5 ) the control of period length by mCRY proteins . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
None of these QTLs were mapped to the chromosomal regions of previously described QTLs for tau and known clock genes ( Clock , mPer 1 , Bmal 1 , mCrv 1 , mCry 2 , mTim , and Csnk1e ) . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The yeast two hybrid screening have demonstrated that CLOCK forms a heterodimer with BMAL 1 . ^^^ This feedback loop is used in the clock output system driving the transcription of arginine vasopressin peptide and serotonin N acetyl transferase by binding the CLOCK BMAL 1 dimer to an E box in their promoters . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Recent studies have shed new light on two mammalian clock components : Mop 3 and Per2 . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Avian homologues of mammalian clock genes Per 2 , Per 3 , Clock , bmal 1 , and MOP 4 have been cloned . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We also show that Clock , Bmal 1 , and Bmal 2 all oscillate under LD and dark dark conditions with similar kinetics , but only Clock is significantly induced while initiating a light induced circadian oscillation in Z 3 cells that have never been exposed to a LD cycle . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
BACKGROUND : In a transcription / translation based autoregulatory feedback loop of vertebrate circadian clock systems , a BMAL 1 CLOCK heterodimer is a positive regulator for the transcription of the negative element gene Per . ^^^ RESULTS : We identified expression of the Per 2 , Bmal 1 , Bmal 2 and Clock genes in the chicken pineal gland . ^^^ CONCLUSION : The functional characterization of the chicken pineal clock molecules supports the key roles of BMAL 1 , BMAL 2 and CLOCK which contribute to the E box dependent transcriptional regulation in the circadian clock system . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
MAPK phosphorylates BMAL 1 at multiple sites , including Ser 527 , Thr 534 , and Ser 599 , in vitro , and BMAL 1 : CLOCK induced transactivation from the E box element is inhibited by expression of a constitutive active form of MAPK kinase in 293 cells . ^^^ These results indicate that BMAL 1 : CLOCK induced transcription is negatively regulated by MAPK mediated phosphorylation of BMAL 1 at Thr 534 and suggest a molecular link between circadian activated MAPK and the clock oscillator . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Here we describe the cloning , sequence , and expression of the circadian Clock and MOP 3 cDNAs of the Spalax ehrenbergi superspecies in Israel . ^^^ We also show that MOP 3 is a bona fide partner of Spalax Clock and that the Spalax Clock / MOP3 dimer is less potent than its human counterpart in driving transcription . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We have found that in rat heart all mammalian homologues of known Drosophila clock genes ( bmal 1 , clock , cry 1 , cry 2 , per 1 , per 2 , per 3 , dbp , hlf , and tef ) show circadian patterns of expression and that the induction of clock output genes ( the PAR [ rich in proline and acidic amino acid residues ] transcription factors dbp , hlf , and tef ) is attenuated in the pressure overloaded hypertrophied heart . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Clock : BMAL 1 and NPAS 2 : BMAL 1 are heterodimeric transcription factors that control gene expression as a function of the light dark cycle . ^^^ Here we show that the DNA binding activity of the Clock : BMAL 1 and NPAS 2 : BMAL 1 heterodimers is regulated by the redox state of nicotinamide adenine dinucleotide ( NAD ) cofactors in a purified system . ^^^ The reduced forms of the redox cofactors , NAD ( H ) and NADP ( H ) , strongly enhance DNA binding of the Clock : BMAL 1 and NPAS 2 : BMAL 1 heterodimers , whereas the oxidized forms inhibit . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Mammalian circadian rhythms are generated by a feedback loop in which BMAL 1 and CLOCK , players of the positive limb , activate transcription of the cryptochrome and period genes , components of the negative limb . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Daily expression patterns of clock genes ( rPer 1 , rPer 2 and BMAL 1 ) and a clock controlled gene Dbp were examined by Northern blot analysis . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
BACKGROUND : CLOCK and BMAL 1 proteins , members of the basic helix loop helix PAS ( PER ARNT SIM ) superfamily of transcription factors which bind to the E box DNA motif , are required for the high level expression of the circadian clock genes period ( per ) and cryptochrome ( cry ) . ^^^ CRY inhibits transcriptional activity of the CLOCK BMAL 1 heterodimer , generating a negative feedback loop that is the core element of the circadian oscillator . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The 24 h expression of seven clock genes ( Bmal 1 , Clock , Per 1 , Per 2 , Cry 1 , Cry 2 , and CK 1 epsilon ) was assayed by in situ hybridization in the suprachiasmatic nucleus ( SCN ) and the pars tuberalis ( PT ) of the pituitary gland , collected every 4 h throughout 24 h , from female Soay sheep kept under long ( 16 h light / 8 h dark ) or short ( 8 h light / 16 h dark ) photoperiods . ^^^ In the SCN , there was a 24 h rhythm in Clock expression , in parallel with Bmal 1 , in antiphase with cycles in Per 1 and Per 2 ; there was low amplitude oscillation of Cry 1 and Cry 2 . ^^^ In the PT , the high amplitude 24 h cycles in the expression of Bmal 1 , Clock , Per 1 , Per 2 , Cry 1 , and Cry 2 , but not CK 1 epsilon , were influenced by photoperiod . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The mouse PERIOD proteins ( mPER 1 and mPER 2 ) , CLOCK , and BMAL 1 undergo robust circadian changes in phosphorylation . ^^^ CLOCK : BMAL 1 heterodimers remain bound to DNA over the circadian cycle . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Interactivating feedback loops within the mammalian clock : BMAL 1 is negatively autoregulated and upregulated by CRY 1 , CRY 2 , and PER 2 . ^^^ Transcription of mBmal 1 was activated by CRY 1 , CRY 2 , and PER 2 , and was repressed by BMAL 1 CLOCK dimers . ^^^ Therefore , CRY , PER 2 , and BMAL 1 CLOCK play bidirectional roles in transcription when they are at high levels by late day and midnight , respectively . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The circadian patterns of gene expression of seven components of the mammalian clock ( bmal 1 , clock , cry 1 , cry 2 , per 1 , per 2 and per 3 ) , as well as three clock output genes ( dbp , hlf and tef ) , were compared in hearts isolated from control and STZ induced diabetic rats . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
All three mPer promoters contain E boxes and respond to the CLOCK / brain and muscle aryl hydrocarbon receptor nuclear translocator ( ARNT ) like protein 1 ( BMAL 1 ) heterodimer . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Moreover , the TTF 1 promoter was inhibited by molecular oscillators of CLOCK and BMAL 1 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The expression of per 3 , cry 1 , 2 , clock , npas 2 , bmal 1 , and casein kinase 1epsilon did not change with sleep deprivation . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
A limited set of genes , Clock , Bmal 1 , mPer 1 , mPer 2 , mCry 1 and mCry 2 , has been shown to be essential for the generation of circadian rhythms in mammals . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Functional CLOCK is not involved in the entrainment of peripheral clocks to the restricted feeding : entrainable expression of mPer 2 and BMAL 1 mRNAs in the heart of Clock mutant mice on Jcl : ICR background . ^^^ To investigate the mechanism that controls circadian rhythms in the mammalian peripheral tissues , we examined the expression rhythm of mPer 2 , BMAL 1 , albumin D site binding protein ( DBP ) , and Rev erbalpha mRNAs in the heart of homozygous Clock mutant mice on Jcl : ICR background under the temporal feeding restriction . ^^^ Unexpectedly , the restricted feeding ( RF ) shifted the circadian phase of both mPer 2 and BMAL 1 mRNA expressions in the heart not only of wild type mice but also of Clock mutant mice . ^^^ Furthermore , in the Clock mutant mice , the amplitude of the circadian expression of mPer 2 and BMAL 1 mRNAs was dramatically increased by the RF . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The proteins Clock and Bmal 1 form a heterodimer which activates the transcription of the Per gene from the E box elements in its promoter region . ^^^ We found that Dec 1 and Dec 2 , basic helix loop helix transcription factors , repressed Clock / Bmal1 induced transactivation of the mouse Per 1 promoter through direct protein protein interactions with Bmal 1 and / or competition for E box elements . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Treatment of cultured hepatic cells ( HepG 2 ) with IFN alpha significantly decreased the protein levels of CLOCK and BMAL 1 , which are positive regulators of circadian output rhythm , then their mRNA levels . ^^^ Aurintricarboxylic acid , a ligand inhibitor of IFN alpha , dose dependently inhibited the IFN alpha induced phosphorylation of the signal transducer and activator of transcription 1 ( STAT 1 ) protein in HepG 2 cells , accompanied by the restoration of Clock and Bmal 1 mRNA levels . ^^^ The continuous administration of IFN alpha significantly decreased CLOCK and BMAL 1 protein levels in the suprachiasmatic nucleus and liver of mice , thereby preventing oscillations in the expression of clock and clock controlled output genes . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Transcripts encoded by the seven known mammalian canonical circadian genes ( Per 1 3 , Cry 1 2 , Bmal 1 and Clock ) , plus the mammalian genetic homologue of the Drosophila canonical gene Timeless , were detected in the uteri and oviducts taken from mice on days 1 4 of pregnancy and in unfertilized oocytes . ^^^ After fertilization , transcripts for Per 1 , Cry 1 , Bmal 1 , Clock and Tim have been detected unambiguously . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Clock and Bmal 1 are essential transcription factors that drive the expression of three period genes ( Per 1 3 ) and two cryptochrome genes ( Cry 1 and Cry 2 ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Here we report that age alters the 24 h expression profile of Clock and its binding partner Bmal 1 in the hamster SCN . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Bmal 1 , another clock gene whose expression oscillates in other tissues , also shows constant expression levels in the testis . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Involvement of CLOCK : BMAL 1 heterodimer in serum responsive mPer 1 induction . ^^^ Based on the previous finding that the process does not involve de novo synthesis of proteins , we postulated the involvement of CLOCK : BMAL 1 heterodimer , a positive regulator of circadian oscillator , in the rapid induction of mPer 1 transcription . ^^^ To test this hypothesis , we utilized CLOCKdelta 19 , a dominant negative mutant , to suppress the function of CLOCK : BMAL 1 in vitro . ^^^ Serum evoked rapid increases of mPer 1 mRNA expression and promoter activity were significantly blunted when CLOCK : BMAL 1 function was interfered with . ^^^ Furthermore , DNA binding activity of CLOCK : BMAL 1 heterodimer to five E boxes of mPer 1 promoter markedly increased shortly after serum shock . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Expression of the circadian clock genes mPer 1 , mPer 2 , Bmal 1 , Clock , mCry 1 , and Npas 2 was constant at all times . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We have cloned Xenopus homologues of the genes thought to be critical for vertebrate clock function , including Clock , Bmal 1 , cryptochromes and period , as well as other rhythmic genes such as nocturnin . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
A full complement of clock genes ( Bmal 1 , Clock , Per 1 , Per 2 , Cry 1 and Cry 2 ) are expressed in the ovine pars tuberalis , and undergo 24 h cyclical expression as observed in a cell autonomous , circadian clock . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Cycle ( Bmal 1 ) is one of the circadian clock genes and the key regulator of the circadian system in many organisms , encoding a bHLH PAS transcription factor . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The ability of CBP and p 300 to interact with pCREB as well as with the clock gene BMAL 1 is discussed , and we propose that CBP and p 300 may interact with , and link , both clock genes and clock controlled genes in the generation of circadian rhythms in mammals . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The vasopressin gene is expressed in the suprachiasmatic nucleus where the basic helix loop helix ( bHLH ) PAS factors CLOCK and MOP 3 regulate circadian expression through interactions with E box sequences . ^^^ However , gel shift analysis shows that the cooperative effect of HIF 1alpha and CLOCK results in MOP 3 binding , but does not involve heterodimerization of HIF 1alpha / CLOCK , at E box A . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
To fill the gap and to better understand the photoperiodic modulation of the SCN state , rats were maintained either under a long photoperiod with 16 h of light and 8 h of darkness per day ( LD 16 : 8 ) or under a short LD 8 : 16 photoperiod , and daily profiles of Per 1 , Cry 1 , Bmal 1 and Clock mRNA in darkness were assessed by in situ hybridization method . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Vertebrate CRY regions responsible for interaction with the CLOCK : BMAL 1 heterodimer and its nuclear localization . ^^^ Mouse mCRY 1 and zebrafish zCRY1a and zCRY 3 belong to the DNA photolyase / Cryptochrome family . mCRY 1 and zCRY1a repress CLOCK : BMAL 1 mediated transcription , whereas zCRY 3 does not . ^^^ Reciprocal chimeras between zCRY1a and zCRY 3 were generated to determine the zCRY1a regions responsible for nuclear translocation , interaction with the CLOCK : BMAL 1 heterodimer , and repression of CLOCK : BMAL 1 mediated transcription . ^^^ Either RD 2a or RD 1 is required for interaction with the CLOCK : BMAL 1 heterodimer , and either RD 1 or RD 2b is required for the nuclear translocation of CRY . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We here report the expression pattern of Period ( Per ) 1 , Per 2 , Per 3 , Cryptochrome ( Cry ) 1 , Cry 2 , Bmal 1 and Clock genes in the SCN of Syrian hamsters when kept under long ( LP ) and short ( SP ) photoperiods . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In the present study we demonstrated that Aa nat mRNA , the key enzyme in melatonin biosynthetic pathway , is affected by illumination in photosensitive pineal , and Aa nat transcription in photosensitive pineal , but not in adult or non photosensitive pineals , is up regulated by BMAL 1 : CLOCK . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Regulation of the PAI 1 promoter by circadian clock components : differential activation by BMAL 1 and BMAL 2 . ^^^ Both CLOCK : BMAL 1 and CLOCK : BMAL 2 heterodimers activate the PAI 1 promoter through requisite proximal ( 565 to 560 bp ) and distal ( 680 to 675 bp ) E box enhancers . ^^^ Although the distal E box overlaps the 4G / 5G polymorphism of the PAI 1 promoter , allelic variation at this site does not influence CLOCK : BMAL 1 and CLOCK : BMAL 2 mediated transactivation . ^^^ Together , CLOCK : BMAL 1 and CLOCK : BMAL 2 make additive contributions to PAI 1 gene transcription . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We present a computational model for the mammalian circadian clock based on the intertwined positive and negative regulatory loops involving the Per , Cry , Bmal 1 , Clock , and Rev Erb alpha genes . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
METHODS : We examined cardiac and aortic mRNA expression for PAI 1 and clock genes ( Per 2 , Bmal 1 , Clock , and Dbp ) every 4 h throughout the day by quantitative reverse transcription polymerase chain reaction , and intervention with the AT 1 receptor antagonist candesartan and equihypotensive hydralazine . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Northern blot analysis and single cosinor analysis revealed that the amplitudes of circadian expression changes of the clock genes ( mPer 2 , Bmal 1 , and dbp ) in the heart , liver , and kidney were significantly decreased in DS H rats compared with a normal salt diet group , except for Bmal 1 in the liver . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The rhythmic expression of clock and clock controlled genes in the rat oviduct was investigated by real time RT PCR . per 1 , per 2 , Clock , Bmal 1 , cry 1 and cry 2 were all expressed in the oviduct . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Using the GT 1 7 cell line , we demonstrate expression of the circadian rhythm genes , clock , BMAL 1 , timeless ( tim ) , period 1 , period 2 , cryptochrome 1 , andcryptochrome 2 . ^^^ With available antibodies , we demonstrated CLOCK , BMAL 1 , and PERIOD 1 protein expression in these cells , with BMAL 1 protein levels showing a rhythmic expression pattern . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Treatment of cultured hepatic cells with IFN alpha caused a significant reduction in Clock and Bmal 1 mRNA levels , which are positive regulators of circadian output rhythm , leading to a decrease in their protein levels . ^^^ The continuous administration of IFN alpha significantly decreased CLOCK and BMAL 1 protein levels in the suprachiasmatic nucleus and liver of mice , thereby preventing oscillations in the expression of clock and clock controlled output genes . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Two basic helix loop helix ( bHLH ) / PAS containing transcription factors , CLOCK and BMAL 1 ( MOP 3 ) , provide the basic drive to the system by activating transcription of negative regulators through E box enhancer elements . ^^^ Our study shows that BMAL 1 and CLOCK proteins are continuously expressed at high levels in the mouse SCN , supporting the hypothesis that rhythmic negative feedback plays the major role in rhythm generation in the mammalian pacemaker . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The endogenous oscillation machinery involves interactive positive and negative transcriptional and posttranslational feedback loops involving the clock genes Per 1 , Per 2 , Per 3 , Clock , Bmal 1 , Cry 1 and Cry 2 . ^^^ To determine whether melatonin feedback control on SCN activity implicates transcriptional regulation of the clock genes , we monitored the expression pattern of Per 1 , 2 , 3 , Bmal 1 , Cry 1 and AVP mRNAs after a single melatonin injection at the end of the subjective day . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
BMAL 1 dependent circadian oscillation of nuclear CLOCK : posttranslational events induced by dimerization of transcriptional activators of the mammalian clock system . ^^^ Mammalian CLOCK and BMAL 1 are two members of bHLH PAS containing family of transcription factors that represent the positive elements of circadian autoregulatory feedback loop . ^^^ We have examined abundance , posttranslational modifications , cellular localization of endogenous and ectopically expressed CLOCK and BMAL 1 proteins . ^^^ Analysis of subcellular localization of CLOCK in embryo fibroblasts of mice carrying different germ line circadian mutations showed that circadian regulation of nuclear accumulation of CLOCK is BMAL 1 dependent . ^^^ This binding dependent coregulation is specific for CLOCK / BMAL1 interaction , as no other PAS domain protein that can form a complex with either CLOCK or BMAL 1 was able to induce similar effects . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Specifically , REV ERBalpha controls the cyclic transcription of Bmal 1 and Clock , the positive limb components . ^^^ In turn , the circadian expression of Rev Erbalpha itself is driven directly by the molecular oscillator : it is activated by BMAL 1 and CLOCK , and repressed by PERIOD1 / 2 and CRYPTOCHROME1 / 2 proteins ( the negative limb members ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Nucleocytoplasmic shuttling and phosphorylation of BMAL 1 are regulated by circadian clock in cultured fibroblasts . ^^^ Nuclear accumulation of BMAL 1 matched nuclear accumulation of CLOCK and the peak of Per 1 transcription . ^^^ In serum shocked mCry1 / mCry2 ( CRY ) deficient fibroblasts , which lack a functional clock , both the cytoplasmic and nuclear BMAL 1 were only present as hyperphosphorylated forms and their circadian nucleocytoplasmic shuttling was absent . ^^^ CONCLUSIONS : We propose that the nucleocytoplasmic shuttling and phosphorylation states of BMAL 1 are regulated by circadian clock , and that this temporally regulated and time delayed nuclear entry of BMAL 1 is important in the maintenance of a stably oscillating clock . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
CONCLUSIONS : The coordinated regulation of the circadian clock genes Cry 1 , Per 2 , and Bmal 1 6 hours after RE and diurnal genes 18 hours after RE in the exercised leg suggest that RE may directly modulate circadian rhythms in human skeletal muscle . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Bmal 1 , Clock , Per 1 , Per 2 , Cry 1 and Cry 2 ) are expressed in the PT , and their expression oscillates through the 24 h light / darkness cycle in a temporal sequence distinct from that in the hypothalamic suprachiasmatic nucleus ( central circadian pacemaker ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Thus , we identify TIC as a regulator of the clock gene circuit . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Advances in chronobiology have identified the suprachiasmatic nucleus ( SCN ) as the center of biological rhythms and the area in which clock genes such as PER 1 , PER 2 , PER 3 , CLOCK , BMAL 1 , TIM , CRY 1 , CRY 2 , tau act to generate and coordinate biological rhythms . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The core circadian oscillator is composed of an autoregulatory transcription translation feedback loop in which CLOCK and BMAL 1 are positive regulators , and Period and Cryptochrome genes act as negative ones . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The expression of Per 1 , Per 2 , Cry 1 , Clock , Bmal 1 , Dec 1 , Dec 2 and c fos was determined 30 and 120 min after treatment in suprachiasmatic nucleus punches by real time reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Comparative analysis of avian BMAL 1 and CLOCK protein sequences : a search for features associated with owl nocturnal behaviour . ^^^ To study the evolution of owl night activity cDNA sequences encoding the circadian core oscillator ( CCO ) proteins BMAL 1 and CLOCK were obtained from barn owl ( Tyto alba ) . ^^^ The predicted proteins showed high sequence identity with their Galliform homologues ( BMAL 1 : 99 % ; CLOCK : 95 . 6 % ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In serum shocked NIH / 3T3 fibroblasts , oscillations in Per 2 , Bmal 1 , and Cry 1 expression persisted with some change in rhythm amplitude during antisense inhibition of CLOCK , demonstrating that feedback interactions between Clock and other core components of the clock mechanism may be regulated differently in SCN2 . 2 cells and fibroblasts . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Basic Helix Loop Helix Per Arnt Sim ( bHLH PAS ) transcription factor , Brain Muscle Arnt Like protein 1 ( BMAL 1 ) , forms a heterodimer with the CLOCK protein . ^^^ Co immunoprecipitation and electrophoretic mobility shift assays ( EMSA ) showed that a BMAL 1 R91A mutant forms a heterodimer with CLOCK , but is unable to support DNA binding in vitro . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The transcriptional activation by a Clock : Bmal 1 heterodimer and the inhibition by Cryptochrome and Period are believed to provide the framework of the feedback loop in mammals . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
DEC 1 and DEC 2 can inhibit CLOCK : BMAL 1 transactivation of the clock gene Per 1 , suggesting that these transcription factors may help regulate circadian timing . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Daily profiles of Per 1 , Per 2 , Cry 1 , Bmal 1 , and Clock mRNA in the SCN of fetuses at the embryonic day ( E ) 19 and of newborn rats at the postnatal day ( P ) 3 and P 10 were assessed by the in situ hybridization method . ^^^ However , no SCN rhythm in their expression was detected ; Per 1 , Cry 1 , and Clock mRNA levels were low , whereas Bmal 1 mRNA levels were high and Per 2 mRNA levels were medium . ^^^ At P 3 , rhythms in Per 1 , Per 2 , Cry 1 , and Bmal 1 , but not in Clock mRNA , were expressed in the SCN . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In this study real time reverse transcription polymerase chain reaction ( RT PCR ) was used in analysis of the expression of the major clock genes Per 1 , Per 2 , Bmal 1 , Cry 1 , Tim ( timeless ) and Clock , as well as of the output genes Dbp and Rev erbalpha in the pancreatic tissue of rats . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In mammals , the core oscillator of the circadian clock is composed of transcription / translation based negative feedback loops regulating the cyclic expression of a limited number of clock genes ( such as Per , Cry , Bmal 1 , etc . ) and hundreds of output genes in a well concerted manner . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We have successfully employed this system in analyzing the effects of clock gene products on the enhancer elements of Per 1 and Bmal 1 promoters . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The expression of clock control genes , such as Bmal 1 and Rev erbalpha , also displays diurnal oscillations in the uterus , but not in bone . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Rora is required for normal Bmal 1 expression and consolidation of daily locomotor activity and is regulated by the core clock in the SCN . ^^^ These results suggest that opposing activities of the orphan nuclear receptors Rora and Rev erb alpha , which represses Bmal 1 expression , are important in the maintenance of circadian clock function . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Several lines of evidence suggest that the time in copula ( TIC ) is not regulated by the known clock mechanism . ^^^ Second , constant light , which abolishes the clock function , does not alter TIC . ^^^ Finally , mutations in the positively acting clock transcription factors , Clk and cyc , do not affect TIC . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Hypercholesterolemia ( 1 % cholesterol diet ) did not significantly affect the daily expression of clock genes ( Per 2 and Bmal 1 ) and clock controlled genes ( Dbp and E4bp4 ) in the liver ( P > 0 . 05 ) ; however , daily expression of the Pai 1 gene and Pai 1 promoter regulating factor genes such as Nr4a1 was significantly upregulated ( P < 0 . 01 ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
It is conjectured that : ( 1 ) the clock ' s 24 h period depends on mRNA stability . ( 2 ) REV ERBalpha suppresses and / or entrains rhythms in peripheral tissues by regulating CRY 1 transcription . ( 3 ) CLK : BMAL 1 oscillations are suppressed in the suprachiasmatic nuclei to enhance oscillations in other proteins . ( 4 ) PER 2 is ineffective at causing phase advances because it is not induced by light during the late night . ( 5 ) The clock is a limit cycle oscillator that shows characteristics of the evening and morning oscillator model . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The model , based on the intertwined positive and negative regulatory loops involving the Per , Cry , Bmal 1 , and Clock genes , can give rise to sustained circadian oscillations in conditions of continuous darkness . ^^^ The mechanism of circadian oscillations relies on the formation of an inactive complex between PER and CRY and the activators CLOCK and BMAL 1 that enhance Per and Cry expression . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
BMAL 1 and CLOCK , two essential components of the circadian clock , are involved in glucose homeostasis . ^^^ We demonstrate a role for Bmal 1 and Clock in the regulation of glucose homeostasis . ^^^ Inactivation of the known clock components Bmal 1 ( Mop 3 ) and Clock suppress the diurnal variation in glucose and triglycerides . ^^^ Gluconeogenesis is abolished by deletion of Bmal 1 and is depressed in Clock mutants , but the counterregulatory response of corticosterone and glucagon to insulin induced hypoglycaemia is retained . ^^^ Bmal 1 and Clock , genes that function in the core molecular clock , exert profound control over recovery from insulin induced hypoglycaemia . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Both promoter regions P 1 ( Rev erbalpha 1 ) and P 2 ( Rev erbalpha 2 ) contain several E box DNA sequences which function as response elements for the core circadian clock components : CLOCK and BMAL 1 . ^^^ The CLOCK BMAL 1 heterodimer stimulates the activity of both P 1 and P 2 promoters in transient transfection assay by 3 6 fold . ^^^ This regulation is conserved in vertebrates since we found that the CLOCK BMAL 1 heterodimer also regulates the zebrafish Rev erbalpha gene . ^^^ In line with these data Rev erbalpha circadian expression was strongly impaired in the livers of Clock mutant mice and in the pineal glands of zebrafish embryos treated with Clock and Bmal 1 antisense oligonucleotides . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Using bioluminescence imaging of Rat 1 fibroblasts transfected with a Bmal 1 : : luc plasmid and primary fibroblasts dissociated from mPer 2 ( Luciferase SV 40 ) knockin mice , we monitored single cell circadian rhythms of clock gene expression for 1 2 weeks . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The E 2 enhancer accounts for most circadian transcriptional drive of the mPer 2 locus by CLOCK : BMAL 1 , is a major site of DNaseI hypersensitivity in this region , and is constitutively bound by a transcriptional complex containing the CLOCK protein . ^^^ Last , genetic analysis with mutations in Clock and Bmal 1 shows that the E 2 enhancer is a target of CLOCK and BMAL 1 in vivo . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Parameter variations that correspond to clock gene mutations reproduce experimental results : With parameter variations mimicking the Bmal 1 ( / ) and the Per 2 ( Brdm 1 ) mutation the oscillations cease to exist . ^^^ Depending on the kinetics of the Per2 / Cry transcriptional activation by BMAL 1 , an increasing BMAL 1 expression leads to either an increase or decrease of the clock period . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
While the sensitivity of wild type mice varies greatly , depending on the time of drug administration , Clock mutant and Bmal 1 knockout mice are highly sensitive to treatment at all times tested . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Pulses of prolactin promoter activity depend on a noncanonical E box that can bind the circadian proteins CLOCK and BMAL 1 . ^^^ Sequence analysis of this fragment identified two potential E boxes ( elements known to bind CLOCK and BMAL 1 circadian proteins ) . ^^^ Furthermore , RT PCR of PRL cells ( pituitary , MMQ , and GH ( 3 ) ) revealed expression of clock and bmal 1 as well as five other clock genes ( per 1 , per 2 , cry 1 , cry 2 , and tim ) , suggesting that the circadian system may function in PRL cells . ^^^ EMSAs revealed that CLOCK and BMAL 1 were able to bind to the E box 133 site in vitro . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
First , luciferase assays followed by a site directed mutagenesis of an E box sequence were used to assess the CLOCK : BMAL 1 transactivated NHE 3 promoter activity . ^^^ A direct binding of CLOCK : BMAL 1 heterodimers to an E box sequences of NHE 3 promoter was confirmed by electrophoretic mobility shift assay ( EMSA ) . ^^^ RESULTS : We present evidence that renal tubular NHE 3 , the Na ( + ) / H ( + ) exchanger critical for systemic electrolyte and acid base homeostasis , is a clock controlled gene regulated directly by CLOCK : BMAL 1 heterodimers in kidneys . ^^^ By analyzing the 5 ' upstream region of the NHE 3 gene , we found an E box critical for the transcription of NHE 3 via the CLOCK : BMAL 1 driven circadian oscillator . ^^^ CONCLUSION : NHE 3 should represent an output gene of the peripheral oscillators in kidney , which is regulated directly by CLOCK : BMAL 1 heterodimers . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We examined mRNA expression for PAI 1 and clock genes ( Per 2 , Bmal 1 , and Clock ) in heart , aorta , liver , and kidney by using the quantitative reverse transcription polymerase chain reaction . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The daily expression patterns of the circadian clock genes Bmal 1 , mPer 1 , and mPer 2 , remain normally circadian coordinated in the livers of these tumor bearing mice . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
For this purpose , the 24 h expression of nine clock genes ( bmal 1 , clock , per 1 , per 2 , per 3 , cry 1 , cry 2 , dec 1 and dec 2 ) and the aa nat gene was monitored under light dark 8 : 16 and light dark 16 : 8 in the rat pineal by using real time RT PCR . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Additionally , expressions of Per 1 , Per 2 , Per 3 , Bmal 1 , Bmal 2 , Clock , Cry 1 , and Cry 2 clock genes were simultaneously detected in single chicken pineal glands . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Molecularly , the biological clock is based on the transcriptional / translational feedback loop of clock genes ( mPer , mCry , Clock , and Bmal 1 ) . ^^^ The aim of the study was to test whether mPer , mCry , Clock , and Bmal 1 are rhythmically expressed in the mouse PT and how the absence of melatonin receptors affects clock gene expression . ^^^ We analyzed clock gene expression by in situ hybridization and compared wild type ( WT ) , melatonin 1 receptor knockout ( MT 1 ko ) , and melatonin 2 receptor knockout ( MT 2 ko ) mice . mPer 1 , mCry 1 , Clock , and Bmal 1 , but not mPer 2 and mCry 2 , were rhythmically expressed in the PT of WT and MT 2 ko mice . ^^^ In the PT of MT 1 ko mice , expression of mPer 1 , mCry 1 , Clock , and Bmal 1 was dramatically reduced . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In mammals , the core molecular mechanism of the oscillator consists of two transcriptional activators , CLOCK and BMAL 1 , and their transcriptional targets , CRYPTOCHROMES ( CRYS ) and PERIODS ( PERS ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
RESULTS : In synchronized mice , mean mRNA levels of clock genes Rev erbalpha , Per 2 , and Bmal 1 varied by 206 , four , and 26 fold , respectively , over the 24 hours in healthy mouse liver ; by 36 , 35 , and 32 fold in the livers of tumor bearing mice ; and by 9 . 4 , 5 . 5 , and sixfold in tumor tissue ( P = . 046 to < . 001 ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core clock Bmal 1 . ^^^ In cultured cells , loss of endogenous RORalpha protein resulted in a dampened circadian rhythm of Bmal 1 transcription , further indicating that RORalpha is a functional component of the cell autonomous core circadian clock . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Hyperlipidemia and peroxisome proliferator activated receptor ( PPAR ) regulation of the PPARalpha gene by CLOCK : BMAL 1 ] . ^^^ Recently , we showed that the CLOCK : BMAL 1 heterodimer regulates the PPARalpha gene via promoter of PPARalpha . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In this study , we indicate the daily expression of clock genes period ( Per ) 1 , 2 , 3 , Bmal 1 , and Clock in whole blood cells in 12 healthy male subjects . ^^^ The peak phase of Per 1 , Per 2 , and Per 3 appeared in the early morning , whereas that of Bmal 1 and Clock appeared in the midnight hours . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We examined the developmental expressions of three clock genes ( Bmal 1 , Cry 1 and Per 1 ) in the Syrian hamster to probe the oscillatory properties of the suprachiasmatic nucleus ( SCN ) over the first 4 days after the completion of SCN neurogenesis . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In this study in mice , we investigated the delayed effects of single and repeated ( i . e . 14 days ) administration of the antidepressant fluoxetine and the psychostimulant cocaine on the brain expression of clock genes Period 1 , Period 2 , Period 3 , Clock , Bmal 1 , Cryptochrome 1 , Cryptochrome 2 , and NPAS 2 ( neuronal PAS domain protein 2 ) , and their putative target gene , serotonin N acetyltransferase . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In mammals the PAS transcription factors Clock , NPAS 2 , and BMAL 1 regulate gene expression as a function of the day night cycle . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Brain and muscle Arnt like protein 1 ( BMAL 1 ) , a component of the molecular clock , regulates adipogenesis . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In the present study , we analysed the expression of three key clock genes , PER 2 , BMAL 1 and REV ERBalpha in PBMCs from ten healthy humans over a 24 h cycle . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Contrary to the hypothesis , the 24 h rhythmic expression of clock genes ( Rev erbalpha , Per 1 , Per 2 , Bmal 1 , Cry 1 ) in the suprachiasmatic nucleus and PT reflected the ambient photoperiod / melatonin signal and not the changing physiology . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The present study exposes oscillations of circadian clock genes [ brain and arylhydrocarbon receptor nuclear translocator like protein 1 ( bmal 1 ) , reverse strand of the c erbaalpha gene ( rev erbaalpha ) , period 2 ( per 2 ) , albumin D element binding protein ( dbp ) ] for isolated adult rat cardiomyocytes in culture . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We studied by quantitative RT PCR the influence of aging on the expression of circadian clock genes ( Clock , Bmal 1 , Cry 1 , 2 , Per 1 3 ) in peripheral tissues ( liver and heart ) of middle aged ( 13 months ) and old ( 27 months ) rats of the Wag / Rij strain exposed to a 12 hours light / 12 hours dark cycle . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
For that purpose , we measured the circadian changes in the expression of clock genes ( Per 1 , Per 2 , Bmal 1 , Clock ) , Dbp ( a clock controlled output gene ) , CREB ( involved in clock signaling ) , cytolytic factors ( granzyme B and perforin ) , and cytokines ( IFN gamma and TNF alpha ) in NK cells enriched from the rat spleen . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Clock related genes including Per 1 , Per 2 , Cry 1 , Bmal 1 , Dec 1 , Dec 2 , Dbp , and Reverbalpha showed robust circadian expression rhythms in the submandibular glands in 12 : 12 hour light dark conditions . ^^^ The Clock mutation resulted in increased or decreased mRNA levels of Per 2 , Bmal 1 , Dec 1 , Dec 2 , and Dbp , and in Cry 1 / background , Cry 2 disruption also increased or decreased mRNA levels of these clock related genes and the amylase 1 gene . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
To investigate whether these oscillations are under a genuine circadian control , we assessed the daily expression of clock genes ( Per 1 , Per 2 , Clock , and Bmal 1 ) , a clock controlled gene ( Dbp ) , cytolytic factors ( granzyme B and perforin ) , and cytokines ( IFN gamma and TNF alpha ) in NK cells enriched from rats maintained in constant darkness ( DD ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Circadian clock control by SUMOylation of BMAL 1 . ^^^ Here , we show that BMAL 1 , an essential transcription factor component of the clock mechanism , is SUMOylated on a highly conserved lysine residue ( Lys 259 ) in vivo . ^^^ SUMOylation of BMAL 1 requires and is induced by CLOCK , the heterodimerization partner of BMAL 1 . ^^^ Ectopic expression of a SUMO deficient BMAL 1 demonstrates that SUMOylation plays an important role in BMAL 1 circadian expression and clock rhythmicity . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Clock gene ( Per 1 , Per 2 and Bmal 1 ) and clock controlled gene ( Vasopressin ) expression in the suprachiasmatic nuclei was assessed over 24 h . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In C57BL / 6J mice , all transcript levels of the clock genes ( Bmal 1 , Per 1 , Per 2 , Cry 1 , Cry 2 , and Dbp ) and adipocytokines ( adiponectin , resistin , and visfatin ) clearly showed 24 h rhythms . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Expression of the clock genes Per 1 and Bmal 1 were elevated in differentiated HC 11 cells , whereas Per 2 mRNA levels were higher in undifferentiated cells . ^^^ This differentiation dependent profile of clock gene expression was consistent with that observed in mouse mammary glands , as Per 1 and Bmal 1 mRNA levels were elevated in late pregnant and lactating mammary tissues , whereas Per 2 expression was higher in proliferating virgin and early pregnant glands . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In mammals , 2 orphan nuclear receptors , REV ERBalpha and RORalpha , play important roles in the transcription of the clock gene Bmal 1 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The expression of five clock genes ( Per 1 , Per 2 , Cry 1 , Rev erbalpha , and Bmal 1 ) was measured by in situ hybridization . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
To ascertain when and how the photoperiod affects the circadian core clock mechanism during ontogenesis , the rhythmic expression of clock genes , namely of Per 1 , Per 2 , Cry 1 and Bmal 1 was determined in 3 , 10 and 20 day old rat pups maintained under either a long photoperiod with 16 h of light and 8 h of darkness per day ( LD 16 : 8 ) or under a short , LD 8 : 16 photoperiod . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Therefore , the present study investigates the levels of three clock gene proteins PER 1 , BMAL 1 and CRY 2 in the murine adrenal cortex and medulla at seven different time points of a 12 hr light / 12 hr dark cycle . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
While in the PT of WT mice mPER 1 , mPER 2 , and mCRY 1 showed a pronounced rhythm , mCRY 2 , CLOCK , and BMAL 1 were constitutively present . ^^^ MT 1 / mice had reduced levels of mPER 1 , mCRY 1 , CLOCK and BMAL 1 , consistent with the earlier reported reduction in mRNA expression of these clock genes . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The three dimensional distributions of PER 1 , PER 2 , CLOCK , and BMAL 1 proteins were recorded along colonic crypts by immunofluorescent confocal imaging . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Northern blots showed that circadian expression of the clock genes mPer 1 , mPer 2 , Clock , and BMAL 1 , and of the clock controlled output gene D site binding protein ( DBP ) , was maintained in Vitamin A deficient mice . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
For this purpose , the 24 h profiles of nine clock genes ( bmal 1 , clock , per 1 , per 2 , per 3 , dec 1 , dec 2 , cry 1 and cry 2 ) and the arylalkylamine N acetyltransferase gene as an indicator of the circadian pacemaker output were compared between light dark periods of 8 : 16 and 16 : 8 h . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The environmental synchronizers are integrated by response elements located in the promoter region of period genes that drive the central oscillator complex ( CLOCK : BMAL 1 and NPAS 2 : BMAL 1 heterodimers ) . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Here , we developed a molecular genetic screen in mammalian cells to identify mutants of the circadian transcriptional activators CLOCK and BMAL 1 , which were uncoupled from CRYPTOCHROME ( CRY ) mediated transcriptional repression . ^^^ Notably , mutations in the PER ARNT SIM domain of CLOCK and the C terminus of BMAL 1 resulted in synergistic insensitivity through reduced physical interactions with CRY . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
A synchronized molecular clock was also demonstrated in equine adipose tissue although oscillation of Bmal 1 was less robust than that of Per 2 and Cry 1 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Lithium treatment of cells leads to rapid proteasomal degradation of Rev erbalpha and activation of clock gene Bmal 1 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Rhythmic CLOCK BMAL 1 binding to multiple E box motifs drives circadian Dbp transcription and chromatin transitions . ^^^ Essentially , the activity of the transcription factors BMAL 1 ( also known as MOP 3 ) and CLOCK is rhythmically counterbalanced by Period ( PER ) and Cryptochrome ( CRY ) proteins to govern time of day dependent gene expression . ^^^ Here we show that circadian regulation of the mouse albumin D element binding protein ( Dbp ) gene involves rhythmic binding of BMAL 1 and CLOCK and marked daily chromatin transitions . ^^^ Thus , the Dbp transcription cycle is paralleled by binding of BMAL 1 and CLOCK to multiple extra and intragenic E boxes , acetylation of Lys 9 of histone H 3 , trimethylation of Lys 4 of histone H 3 and a reduction of histone density . ^^^ The rhythmic conversion of transcriptionally permissive chromatin to facultative heterochromatin relies on the presence of functional BMAL 1 CLOCK binding sites . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The basic helix loop helix ( bHLH ) PAS domain containing transcription factors CLOCK and BMAL 1 are two major components of the circadian molecular oscillator . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Our previous data indicated a temporal association between the expressions of chicken Bmal 1 clock gene and Aanat suggesting a functional molecular link between them . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We have assessed evidence for linkage and association involving polymorphisms in 10 circadian clock genes ( ARNTL , CLOCK , CRY 2 , CSNK1epsilon , DBP , GSK3beta , NPAS 2 , PER 1 , PER 2 , and PER 3 ) to BPAD . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
When E box activating transcription factors , CLOCK and BMAL 1 , were coexpressed , each of both proteins showed two molecular forms . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We demonstrate that PPARalpha plays a specific role in the peripheral circadian control because it is required to maintain the circadian rhythm of the master clock gene brain and muscle Arnt like protein 1 ( bmal 1 ) in vivo . ^^^ We further demonstrate that fenofibrate induces circadian rhythm of clock gene expression in cell culture and up regulates hepatic bmal 1 in vivo . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In this study , we show robust and coordinated expression of circadian oscillator genes ( Npas 2 , Bmal 1 , Per 1 3 , and Cry 1 2 ) and clock controlled downstream genes ( Rev erb alpha , Rev erb beta , Dbp , E4bp4 , Stra 13 , and Id 2 ) in murine brown , inguinal , and epididymal ( BAT , iWAT , and eWAT ) adipose tissues . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Daily profiles of Per 1 , Per 2 , Cry 1 , Bmal 1 , and Clock mRNA and of AVP heteronuclear ( hn ) RNA as an indicator of AVP gene transcription were assessed in the SCN of fetuses at E 20 and of newborn rats at P 1 and P 2 by the in situ hybridization method . ^^^ At P 2 , marked rhythms of Per 1 , Per 2 , and Bmal 1 and a forming rhythm of Cry 1 , but not of Clock , expression were present . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Mammalian CLOCK ( NPAS 2 ) , BMAL 1 and CRYPTOCHROMEs are core components of the circadian oscillatory mechanism . ^^^ We have examined the effect of CRYPTOCHROMEs on posttranslational modifications and intracellular distribution of endogenous and ectopically expressed CLOCK ( NPAS 2 ) and BMAL 1 proteins . ^^^ This effect was CRY specific , as other known repressors of CLOCK / BMAL1 and NPAS2 / BMAL 1 transcriptional activity were not able to induce similar effects . ^^^ Importantly , the posttranslational modifications and intracellular distribution of CLOCK and BMAL 1 proteins were critically impaired in the tissues of mice with targeted disruption of both Cry genes , thus confirming the suggested role of CRY in clock function in vivo . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Two transcription factors , CLOCK and BMAL 1 , are believed to be essential components of the circadian clock . ^^^ Our data challenge a central feature of the current mammalian circadian clock model regarding the necessity of CLOCK : BMAL 1 heterodimers for clock function . . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
BMAL 1 , the heterodimerization partner of CLOCK , enhances HAT function . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
To investigate avian clock gene expression , partial cDNA sequences of six mammalian clock gene homologs ( Bmal 1 , Clock , Per 2 , Per 3 , Cry 1 , and Cry 2 ) and a novel avian cryptochrome gene ( Cry 4 ) were cloned from the house sparrow , a model system in circadian research . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Mapping of binding regions for the circadian regulators BMAL 1 and CLOCK within the mouse Rev erbalpha gene . ^^^ Time of day dependent inhibition of the activity of BMAL 1 and CLOCK by PERIOD ( PER ) and CRYPTOCHROME ( CRY ) proteins provokes consequent rhythmic gene expression . ^^^ Genetically linked to the molecular oscillator , it was identified as a repressor of the Bmal 1 and Clock genes . ^^^ In an attempt to understand the circadian regulation of the mouse Rev erbalpha gene , we developed a screening technique for BMAL 1 and CLOCK binding regions consisting of chromatin immunoprecipitation . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
From the 4 clock genes ( Per 1 , Per 2 , Cry 1 and Bmal 1 ) studied , the expression of Bmal 1 and Per 1 was rhythmic already in 1 day old rats ; at this age , the Per 2 mRNA rhythm just started to form and no rhythm in Cry 1 expression was detected . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Signaling mediated by the dopamine D 2 receptor potentiates circadian regulation by CLOCK : BMAL 1 . ^^^ Here , we report that signaling mediated by the dopamine D 2 receptor ( D2R ) enhances the transcriptional capacity of the CLOCK : BMAL 1 complex . ^^^ Importantly , CLOCK : BMAL 1 dependent activation and light inducibility of mPer 1 gene transcription is drastically dampened in retinas of D2R null mice . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Cells were collected , and their transcriptomes were subjected to multiplex single cell RT PCR for the core clock genes Period ( Per ) 1 and 2 , Cryptochrome ( Cry ) 1 and 2 , Clock , and Bmal 1 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In HEK 293 cells , introduction of brain and muscle aryl hydrocarbon receptor nuclear translocator like protein 1 ( bmal 1 ) / clock enhanced mouse type 2 collagen first intron reporter activity without affecting promoter activity , with reduction effected by either per 1 or per 2 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Remarkably , the last 43 aa of BMAL 1 are required for transcriptional activation , as well as for association with the circadian transcriptional repressor CRYPTOCHROME 1 ( CRY 1 ) , depending on the coexistence of CLOCK protein . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Transcripts for core oscillator elements ( Arntl , Clock , Per 1 , Per 2 , and Cry 1 ) were present in the ovary as indicated by quantitative real time RT PCR . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
We found in the 90 % of gestation capuchin monkey fetus expression of the clock genes Bmal 1 , Per 2 , Cry 2 , and Clock in the SCN , adrenal , pituitary , brown fat , and pineal . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Early aging and age related pathologies in mice deficient in BMAL 1 , the core componentof the circadian clock . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
In contrast to the SCN , per 1 and per 2 expression , as well as Bmal 1 expression in liver and skeletal muscle , together with plasma corticosterone , was arrhythmic in Clock ( Delta 19 ) + MEL mutant mice in normal light dark conditions . npas 2 mRNA was also arrhythmic in liver but rhythmic in muscle . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
A model cell line was established with Rat 1 fibroblasts expressing CLuc driven by the promoter of a circadian clock gene , Bmal 1 . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Endogenous EZH 2 , a polycomb group enzyme that methylates lysine 27 on histone H 3 , co immunoprecipitates with CLOCK and BMAL 1 throughout the circadian cycle in liver nuclear extracts . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
The mouse Clock gene encodes a basic helix loop helix PAS transcription factor , CLOCK , that acts in concert with BMAL 1 to form the positive elements of the circadian clock mechanism in mammals . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
Kcnma 1 ( / ) mice show normal expression of clock genes such as Arntl ( Bmal 1 ) , indicating a role for BK channels in SCN pacemaker output , rather than in intrinsic time keeping . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
BMAL 1 shuttling controls transactivation and degradation of the CLOCK / BMAL1 heterodimer . ^^^ CLOCK and BMAL 1 are bHLH PAS containing transcription factors that bind to E box elements and are indispensable for expression of core circadian clock components such as the Per and Cry genes . ^^^ A key step in expression is the heterodimerization of CLOCK and BMAL 1 and their accumulation in the nucleus with an approximately 24 h periodicity . ^^^ We show here that nucleocytoplasmic shuttling of BMAL 1 is essential for transactivation and for degradation of the CLOCK / BMAL1 heterodimer . ^^^ Transient transfection experiments revealed that heterodimerization of CLOCK and BMAL 1 accelerates their turnover , as well as E box dependent clock gene transcription . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
A new reporter system for monitoring expressions of two clock genes , Per 1 and Bmal 1 , from a single tissue in culture was developed in mice . ^^^
Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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Interacting proteins: O15516 and O00327	Pubmed	SVM Score :0.0
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