| In addition , p 60 Src or Lyn kinase phosphorylated recombinant RGS 16 in vitro , and RGS 16 underwent phosphorylation in the presence of constitutively active Src ( Y529F ) in EGFR ( ) CHO K 1 cells . ^^^ Src mediated RGS 16 tyrosine phosphorylation promotes RGS 16 stability . ^^^ Blockade of endogenous Src activity by selective inhibitors attenuated RGS 16 phosphorylation induced by pervanadate or receptor stimulation . ^^^ Furthermore , the rate of RGS 16 degradation was reduced in cells expressing active Src or treated with pervanadate or a G protein coupled receptor ligand ( CXCL 12 ) . ^^^ These results suggest that Src mediates RGS 16 tyrosine phosphorylation , which may promote RGS 16 stability . . ^^^ |