| Interacting proteins: Q9UEU0 and O15400 |
Pubmed |
SVM Score :0.0 |
| We report that syntaxin 7 , syntaxin 8 , vti1b and endobrevin / VAMP 8 form a complex that functions in the fusion of late endosomes . ^^^ Substitution experiments , sequence and structural comparisons revealed that each protein occupies a unique position in the complex , with syntaxin 7 corresponding to syntaxin 1 , and vti1b and syntaxin 8 corresponding to the N and C terminal domains of SNAP 25 , respectively . ^^^ |
|
| Interacting proteins: Q9UEU0 and O15400 |
Pubmed |
SVM Score :0.0 |
| Here we report the 1 . 9 A resolution crystal structure of an endosomal SNARE core complex containing four SNAREs : syntaxin 7 , syntaxin 8 , vti1b and endobrevin / VAMP 8 . ^^^ |
|
| Interacting proteins: Q9UEU0 and O15400 |
Pubmed |
SVM Score :0.0 |
| The N terminal domains of syntaxin 7 and vti1b form three helix bundles that differ in their ability to regulate SNARE complex assembly . ^^^ The SNAREs syntaxin 7 , syntaxin 8 , vti1b , and endobrevin / VAMP8 function in the fusion of late endosomes . ^^^ Here we show that the N terminal regions of syntaxin 7 , syntaxin 8 , and vti1b contain well folded alpha helical domains . ^^^ Multidimensional NMR spectroscopy revealed that in syntaxin 7 and vti1b , the domains form three helix bundles resembling those of syntaxin 1 , Sso1p , and Vam3p . ^^^ Only syntaxin 7 adopts a closed conformation , whereas in vti1b and syntaxin 8 , the N terminal domains do not interact with the adjacent SNARE motifs . ^^^ |
|
| Interacting proteins: Q9UEU0 and O15400 |
Pubmed |
SVM Score :0.0 |
| A SNARE complex consisting of vti1b , syntaxin 8 , syntaxin 7 , and endobrevin / VAMP 8 which is required for fusion of late endosomes in vitro has been identified recently . ^^^ Here , we generated mice deficient in vti1b to study the function of this protein in vivo . vti1b deficient mice had reduced amounts of syntaxin 8 due to degradation of the syntaxin 8 protein , while the amounts of syntaxin 7 and endobrevin did not change . ^^^ |
|
| Interacting proteins: Q9UEU0 and O15400 |
Pubmed |
SVM Score :0.0 |
| A trans SNARE complex consisting of Q SNAREs syntaxin 7 , Vti1b and syntaxin 8 and the R SNARE VAMP 8 has been shown by others to be responsible for homotypic fusion of late endosomes . ^^^ |
|