Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Recently , in vitro studies have shown that members of the N methyl aspartate ( NMDA ) class of glutamate receptors interact with a synapse associated protein , SAP 90 ( PSD 95 ) . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
With the advent of powerful new microchemical tools and molecular genetic methods , three new classes of proteins have been identified in the PSD at glutamatergic synapses : the PSD 95 family , the NR2B subunit of the NMDA type glutamate receptor , and densin 180 . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
The distributions of the presynaptic marker synaptophysin , the AMPA type glutamate receptor subunit GluR 1 , and the putative NMDA receptor clustering protein PSD 95 were not affected by blockade . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
To determine their roles in the assembly of glutamatergic postsynaptic sites , we studied the distributions of NMDA and AMPA type glutamate receptors ; the NMDA receptor interacting proteins alpha actinin 2 , PSD 95 , and chapsyn ; and the PSD 95 associated protein GKAP during the development of hippocampal neurons in culture . ^^^ These results suggest that synapse development proceeds by formation of a postsynaptic scaffold containing PSD 95 and GKAP in concert with presynaptic vesicle clustering , followed by regulated attachment of glutamate receptor subtypes to this scaffold . . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Postsynaptic localization of N methyl D aspartate type glutamate receptors may be mediated by the synapse associated proteins ( SAPs ) SAP 90 , SAP 102 , and chapsyn 110 . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
NMDA receptors have been found to bind to the PSD 95 family of proteins , whereas AMPA receptors interact with the PDZ domain containing protein GRIP ( glutamate receptor interacting protein ) . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Synaptic NMDA type glutamate receptors are anchored to the second of three PDZ ( PSD 95 / Discs large / ZO 1 ) domains in the postsynaptic density ( PSD ) protein PSD 95 . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Synaptic vesicle proteins , AMPA and NMDA type glutamate receptors , GABAA receptors , and the putative synapse organizing proteins PSD 95 , GKAP , and gephyrin formed numerous clusters at synaptic sites . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Expression of PSD 95 decreased the sensitivity of the NMDA receptor channels to L glutamate . ^^^ Mutational studies showed that the interaction between the COOH terminus of the epsilon 2 subunit of the NMDA receptor and the second PSD 95 / Dlg / Z0 1 domain of PSD 95 is critical for the decrease in glutamate sensitivity . ^^^ In addition to this , our results suggest that PSD 95 plays a protective role against neuronal excitotoxicity by decreasing the glutamate sensitivity of the channels and by inhibiting the protein kinase C mediated potentiation of the channels . . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
In cultured hippocampal neurons , MALS proteins are clustered together with PSD 95 and NMDA type glutamate receptors , consistent with a postsynaptic localization for MALS proteins . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
In cultured cortical neurons , suppressing the expression of the NMDAR scaffolding protein PSD 95 ( postsynaptic density 95 ) selectively attenuated excitotoxicity triggered via NMDARs , but not by other glutamate or calcium ion ( Ca2+ ) channels . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
By virtue of their multiple protein binding domains ( e . g . , three PDZs in PSD 95 and seven PDZs in GRIP ) , PSD 95 and GRIP can function as multivalent proteins that organize a specific cytoskeletal and signaling complex associated with each class of glutamate receptor . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Postsynaptic density 95 ( PSD 95 / SAP 90 ) is a membrane associated guanylate kinase ( GK ) PDZ protein that scaffolds glutamate receptors and associated signaling networks at excitatory synapses . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
The NMDA subtype of glutamate receptor is physically associated with the postsynaptic density protein PSD 95 at glutamatergic synapses . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
The PDZ domain containing proteins , such as PSD 95 and GRIP , have been suggested to be involved in the targeting of glutamate receptors , a process that plays a critical role in the efficiency of synaptic transmission and plasticity . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
In this report , we demonstrate that glutamate receptors and PKA are recruited into a macromolecular signaling complex through direct interaction between the MAGUK proteins , PSD 95 and SAP 97 , and AKAP79 / 150 . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
PSD 95 , DLG , ZO 1 ( PDZ ) domain mediated protein interactions have been shown to play important roles in the regulation of glutamate receptor function at excitatory synapses . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
We find that glutamate input is necessary for clustering the AMPA type glutamate receptor but not for clustering the NMDA receptor or the associated PSD 95 family scaffold in GABAergic cells ; GABA input is not necessary for clustering the GABA ( A ) receptor or gephyrin in pyramidal cells . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
PSD 95 expression enhanced postsynaptic clustering and activity of glutamate receptors . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
In vivo , SAP 90 tightly binds kainate receptor subunits , while SAP 97 is only weakly associated , suggesting that this glutamate receptor differentially associates with SAP90 / PSD 95 family members . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Interdomain chaperoning between PSD 95 , Dlg , and Zo 1 ( PDZ ) domains of glutamate receptor interacting proteins . ^^^ The multiple PSD 95 , Dlg , and Zo 1 ( PDZ ) domain protein , glutamate receptor interacting protein ( GRIP ) , is involved in the clustering and trafficking of the alpha amino 3 hydroxy 5 methyl 4 isoxazole propionate receptor by directly binding to the cytoplasmic tail of the receptor ' s GluR 2 subunit . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
We also studied the synaptic localization of the two postsynaptic density proteins PSD 95 and glutamate receptor interacting protein ( GRIP ) . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
When coexpressed in human embryonic kidney 293 cells , PrRP receptor was able to coimmunoprecipitate the three PDZ domain proteins known to interact with AMPA receptors : glutamate receptor interacting protein ( GRIP ) , AMPA binding protein ( ABP ) , and protein that interacts with C kinase ( PICK 1 ) , but not the PDZ domain protein PSD 95 , which does not interact with AMPA receptors . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Tyrosine phosphorylation of ionotropic glutamate receptors by Fyn or Src differentially modulates their susceptibility to calpain and enhances their binding to spectrin and PSD 95 . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Hippocampal mossy fibers induce assembly and clustering of PSD 95 containing postsynaptic densities independent of glutamate receptor activation . ^^^ Antibodies to a prominent postsynaptic density ( PSD ) scaffold protein , PSD 95 , identified large ( > 1 microm ) and irregularly shaped PSD assemblies that codistributed with synapsin 1 or metabotropic glutamate receptor 7b ( mGluR7b ) immunolabeled MF terminals in area CA 3 . ^^^ The results indicate that MF axons can induce the assembly and clustering of PSD 95 containing postsynaptic complexes , displaying a normal subcellular and tissue distribution , by mechanisms that are independent of ionotropic glutamate receptor activation . . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Stargazin is the first transmembrane protein known to associate with AMPA ( alpha amino 3 hydroxy 5 methylisoxazole 4 propionate ) glutamate receptors ( AMPARs ) and regulate their synaptic targeting by two distinct mechanisms , specifically via delivery of AMPARs to the surface membrane and synaptic targeting of these receptors by binding to PSD 95 / SAP 90 and related PDZ proteins . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
In this investigation , we report identification and characterization of a 95 kDa postsynaptic density protein ( PSD 95 ) / discs large / ZO 1 ( PDZ ) domain containing protein termed tamalin , also recently named GRP 1 associated scaffold protein ( GRASP ) , that interacts with group 1 metabotropic glutamate receptors ( mGluRs ) . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
We quantitatively defined the contributions of carboxyl terminal residues to binding selectivity of the prototypic interactions of the PDZ domains of postsynaptic density protein 95 ( PSD 95 ) and its homolog synapse associated protein 90 ( SAP 102 ) with the NR2b subunit of the N methyl d aspartate type glutamate receptor . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Here , we identify palmitate cycling on PSD 95 at the synapse and find that palmitate turnover on PSD 95 is regulated by glutamate receptor activity . ^^^ We also find that rapid glutamate mediated AMPA receptor internalization requires depalmitoylation of PSD 95 . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
DeSouza and Ziff discuss the evidence that the reversible palmitoylation of the postsynaptic density protein PSD 95 may result in the movement of AMPA type glutamate receptors into and out of lipid raft domains , ultimately controlling AMPA receptor accumulation at the postsynaptic membrane . . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Delta catenin forms stable complexes with excitatory neurotransmitter receptors including ionotropic N methyl D aspartic acid receptor 2A ( NR2A ) , metabotropic glutamate receptor 1alpha ( mGluR1alpha ) , as well as PSD 95 in vivo . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
The membrane associated guanylate kinase PSD 95 scaffolds N methyl d aspartate receptors to cytoplasmic signaling molecules , and associates with other glutamate receptors at central synapses . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
For glutamate receptor 6 ( GluR 6 ) kainate receptors , two unrelated proteins , concanavalin A ( Con A ) and postsynaptic density protein 95 ( PSD 95 ) , bind to extra and intracellular domains , respectively , but are reported to exert similar effects on GluR 6 desensitization behaviour . ^^^ The rate of desensitization elicited by 10 mM L glutamate was similar in control ( taufast = 5 . 5 + / 0 . 4 ms ) , Con A treated patches ( taufast = 6 . 1 + / 0 . 5 ms ) and patches containing PSD 95 and GluR 6 receptors ( taufast = 4 . 7 + / 0 . 6 ms ) . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
We have found that DR reduced the expression of the NMDA receptor 2A subunit and its associated protein PSD 95 ( postsynaptic density 95 ) , of GRIP ( AMPA glutamate receptor interacting protein ) , and of the biosynthetic enzyme GAD ( glutamic acid decarboxylase ) . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
By using a sensitive immunogold procedure , we have investigated the organization of two synaptic scaffolding molecules , PSD 95 and PSD 93 , as well as N methyl D aspartate ( NMDA ) and alpha amino 3 hydroxy 5 methylisoxazole 4 proprionic acid ( AMPA ) receptors , at these heterogeneous glutamate signaling sites . ^^^ Although the lateral dendrites of mitral and tufted cells have been reported to respond to glutamate , they did not display significant plasma membrane labeling for the NR 1 subunit or for PSD 95 , suggesting that the physiological effects of glutamate at these sites are mediated by NMDA autoreceptors that are not clustered and occur only at a low density on the dendritic surface . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
The fragile 10 mental retardation protein is required for type 1 metabotropic glutamate receptor dependent translation of PSD 95 . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
This preparation was used to assess the association of alpha amino 3 hydroxy 5 methylisoxazole 4 propionate ( AMPA ) type glutamate receptors with the PSD 95 containing complex . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
PSD 95 is a major scaffolding protein of the postsynaptic density , tethering NMDA and AMPA type glutamate receptors to signaling proteins and the neuronal cytoskeleton . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Each family of PSD proteins is selective for a given glutamate receptor subtype , the most well characterized being the NMDA receptor binding proteins PSD 93 , PSD 95 , NF L , and SAP 102 . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
In hippocampal slice cultures , the expression of PSD 95 green fluorescent protein ( PSD 95 GFP ) increases AMPAR currents by selectively delivering glutamate receptor 1 ( GluR 1 ) containing receptors to synapses , thus mimicking long term potentiation ( LTP ) . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
In this study , we investigated dopamine glutamate interactions by measuring the expression of transcripts encoding the subunits for the ionotropic glutamate receptors ( NMDA , AMPA and kainate ) and five NMDAR associated intracellular proteins , PSD 93 , PSD 95 , SAP 102 , NF L and yotiao in the dopaminergic neurons in the substantia nigra pars compacta ( SNc ) of subjects with schizophrenia and a comparison group . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
We review the potential roles in brain plasticity of two membrane associated guanylate kinases ( MAGUKs ) , SAP 102 and PSD 95 , which form a scaffold for the ion passing glutamate receptors at the postsynaptic density , and we consider the known functional significance of these molecules in subunit switching . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
In the postsynaptic density of neuronal excitatory synapses , PDZ proteins such as PSD 95 organize glutamate receptors and their associated signalling proteins and determine the size and strength of synapses . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Clusters of PSD 95 and subunits of AMPA type and NMDA type glutamate receptors accumulate in spines of mutant neurons by day 10 in vitro , whereas in wild type neurons they are still mostly located in dendritic shafts . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
RESULTS : At three days of ethanol withdrawal , we found region specific and sex selective alterations in levels of GAD ( glutamic acid decarboxylase , GABA synthetic enzyme ) , GABA and glutamate transporters , and the synapse associated proteins HSP 70 , PSD 95 , and synaptophysin . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Interestingly , the properties of basal neurotransmission are normal in the Mecp 2 null mice , consistent with our observations that the levels of expression of synaptic and cytoskeletal proteins , including glutamate receptor subunits GluR 1 and GluR 2 , PSD 95 , synaptophysin 1 , synaptobrevin 2 , synaptotagmin 1 , MAP 2 , betaIII tubulin and NF 200 , are not significantly altered . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
A reduction in PSD 95 at glutamate synapses of the molecular layer may have a deleterious impact on information flow to other hippocampal regions via granule cells and their projecting mossy fibres . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
In short term neocortical cultures , concentrations of epidermal growth factor and amphiregulin ( 2 9 pM ) decreased the expression of glutamate receptor interacting protein 1 ( GRIP 1 ) and synapse associated protein 97 kDa ( SAP 97 ) without affecting postsynaptic density 95 ( PSD 95 ) levels and glial proliferation . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Among the earliest changes in synaptic composition in APP mutant neurons were reductions in PSD 95 , a protein involved in recruiting and anchoring glutamate receptor subunits to the post synaptic density . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Kainate receptor glutamate receptor 6 ( GluR 6 ) binds to the postsynaptic density protein 95 ( PSD 95 ) , which in turn anchors mixed lineage kinase 3 ( MLK 3 ) via SH 3 domain in rat brain tissue . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
After reviewing its role as a signaling receptor , we discuss the connection between LRP and the NMDA glutamate receptor via the post synaptic density 95 ( PSD 95 ) neuronal scaffold protein and the implications it may have for memory and cognition . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Immunostaining analysis of cortical cultured neurons revealed that Abeta treatment induces concomitant decreases in PSD 95 at synapses and in the surface expression of the AMPAR glutamate receptor subunit 2 . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Synapse associated protein 97 ( SAP 97 ) and postsynaptic density 95 ( PSD 95 ) are closely related membrane associated guanylate kinase homologs ( Maguks ) implicated in the synaptic targeting and anchoring of alpha amino 5 methyl 3 hydroxy 4 isoxazolepropionic acid ( AMPA ) selective glutamate receptors . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Postsynaptic density 95 ( PSD 95 ) , a PSD 95 / Discs large / zona occludens 1 ( PDZ ) domain containing scaffold protein , clusters many signaling molecules near NMDA type glutamate receptors in the postsynaptic densities . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
These sexual morphological differences correlate with differences in the postsynaptic dense protein ( PSD 95 ) as well as the spectrum of glutamate receptors induced by GC treatment in male and female mice , including NMDA , AMPA , and KA receptors . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
These data confirm the abundance of calcium / calmodulin dependent protein kinase 2 and PSD 95 and reveal unexpected stoichiometric ratios between glutamate receptors , scaffold proteins , and signaling molecules in the PSD . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
However , activity dependent phosphorylation of substrates at synapses was highly selective in that the glutamate receptor subunits NR2B and GluR 1 were poorly phosphorylated whereas PSD 95 and Stargazin , proteins implicated in the scaffolding and trafficking of AMPA receptors , were robustly phosphorylated . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
Applying semi quantitative immunoblotting , we found that in the whole tetanized hippocampus , synaptic expression of the N methyl D aspartate and alpha amino 3 hydroxyl 5 methyl 4 isoxazole propionate receptor subunits ( NR 1 , NR2A , glutamate receptor 1 ) and their associated partners , e . g . synaptic associated protein 97 , postsynaptic density protein 95 , alpha subunit of Ca2+ / calmodulin dependent protein kinase 2 , neuronal nitricoxide synthase , increased 180 min post high frequency stimulation . ^^^
Interacting proteins: O15399 and P78352 Pubmed SVM Score :0.0
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