| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.50411155 |
| LCB 2 appears to be unstable unless it is associated with LCB 1 . 0.50411155^^^ |
|
| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Genetic analysis of over 60 such strains indicated only two complementation classes , lcb 1 and lcb 2 . ^^^ Since these metabolites represent the first , second , and last components , respectively , of the long chain base biosynthetic pathway , it is likely that the LCB 1 and LCB 2 genes are involved in the first step of long chain base synthesis . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Mutant strains of S . cerevisiae that require a sphingolipid long chain base for growth fall into two genetic complementation groups , LCB 1 and LCB 2 . ^^^ Membrane preparations from both lcb 1 and lcb 2 mutant strains exhibited negligible SPT activity when tested in vitro . ^^^ We conclude that the Lcb phenotype of these mutants results from a missing or defective SPT , an activity controlled by both the LCB 1 and LCB 2 genes . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| The LCB 2 gene of Saccharomyces and the related LCB 1 gene encode subunits of serine palmitoyltransferase , the initial enzyme in sphingolipid synthesis . ^^^ Here we demonstrate that overproduction in Saccharomyces cerevisiae requires expression of LCB 1 , a previously isolated yeast gene , and LCB 2 , the isolation and characterization of which we describe . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Human expressed sequence tag ( EST ) clones are similar to the two yeast genes for synthesis of long chain bases , LCB 1 and LCB 2 , which are believed to encode two subunits of SPT [ Buede , R . , Pinto , W . ^^^ We have cloned and characterized two complete human and murine cDNA sequences named hLCB 1 & mLCB 1 and hLCB 2 & mLCB 2 , respectively , similar to the yeast LCB 1 and LCB 2 genes . ^^^ Human embryonic kidney cells ( HEK 293 ) transfected with murine sequences of LCB 1 ( mLCB 1 ) and LCB 2 ( mLCB 2 ) independently and in coexpression showed an overexpression of the transcripts on the mRNA and protein level . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| For SPT activity , the yeast Saccharomyces cerevisiae requires two genes , LCB 1 and LCB 2 . ^^^ We isolated mammalian LCB 1 cDNA homologs from mouse and Chinese hamster ovary ( CHO ) cells and an LCB 2 cDNA homolog from CHO cells . ^^^ The mammalian LCB 1 proteins are predicted to have about 35 % amino acid identity to the yeast Lcb 1 protein , whereas the CHO LCB 2 protein is predicted to have about 40 % amino acid identity to the yeast Lcb 2 protein . ^^^ Northern blot analysis of mRNA isolated from various mouse tissues revealed that the tissue distribution of both LCB 1 and LCB 2 messengers followed a similar pattern . ^^^ Transfection of an SPT defective CHO mutant strain with a CHO LCB 1 expressing plasmid restored both SPT activity and de novo sphingolipid synthesis to the wild type levels , whereas transfection of the mutant strain with a CHO LCB 2 expressing plasmid did not exhibit any recovery effects , indicating that the SPT defect in the mutant cells is specifically complemented by the CHO LCB 1 homolog . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| The recent isolation of cDNAs for SPT ( i . e . , LCB 1 and LCB 2 ) allow molecular regulation studies to be performed . ^^^ In CHK , transcripts for both LCB 1 ( 3 . 0 kb ) and LCB 2 ( 2 . 3 kb ) are evident by Northern blot analysis , and UVB exposure ( 23 mJ / cm2 ) induces a delayed 1 . 8 to 3 . 3 fold increase in LCB 2 mRNA levels ( P < 0 . 01 ) 48 h after treatment versus non irradiated control cells . ^^^ In contrast , neither LCB 1 nor a second LCB 2 transcript ( 8 . 0 kb ) changed significantly . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Genetic and biochemical evidence for complex formation of the LCB 1 protein with the LCB 2 protein for serine palmitoyltransferase . ^^^ Expression of an affinity peptide tagged LCB 1 protein in LY B cells caused the endogenous LCB 2 protein to adsorb to a tag affinity matrix . ^^^ In addition , an anti hamster LCB 2 protein antibody co immunoprecipitated both SPT activity and the wild type LCB 1 protein with the LCB 2 protein . ^^^ Moreover , these results demonstrate that the SPT enzyme comprises both the LCB 1 and LCB 2 proteins . . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Analyses of these polypeptides establish conclusively that they are murine LCB 1 and LCB 2 , mammalian homologs of two yeast proteins that have been genetically linked to sphingolipid biosynthesis . ^^^ CONCLUSION : The ability of myriocin containing matrices to bind factors that have SPT activity and the exclusive isolation of LCB 1 and LCB 2 as myriocin binding proteins demonstrates that the two proteins are directly responsible for SPT activity and that myriocin acts directly upon these polypeptides . . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| This enzyme contains at least two different subunits , named the LCB 1 and LCB 2 proteins . ^^^ The endogenous LCB 2 protein was co purified with the tagged LCB 1 protein in purification of SPT . ^^^ SDS polyacrylamide gel electrophoretic analysis of purified SPT , together with immunoprecipitation analysis of metabolically labeled LCB proteins , strongly suggested that the SPT enzyme consisted of the LCB 1 and LCB 2 proteins with a stoichiometry of 1 : 1 . . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Nicotinamide increased the levels of human LCB 1 and LCB 2 mRNA , both of which encode subunits of SPT . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| This enzyme is a water soluble homodimeric protein unlike eukaryotic enzymes , known as heterodimers composed of tightly membrane bound subunits , named LCB 1 and LCB 2 . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Mutations in the yeast LCB 1 and LCB 2 genes , including those corresponding to the hereditary sensory neuropathy type 1 mutations , dominantly inactivate serine palmitoyltransferase . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Although in HSN 1 lymphoblasts , the C133Y and C133W mutations do not alter the steady state levels of LCB 1 and LCB 2 subunits , they result in reduced SPT activity and sphingolipid synthesis . ^^^ In addition , we demonstrate that in CHO cells the mutant LCB 1 proteins , similar to the normal LCB 1 , can interact with the wild type LCB 2 subunit . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| The key enzyme for de novo sphingolipid synthesis is serine palmitoyltransferase , which consists of two different subunits , named LCB 1 and LCB 2 proteins . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Serine palmitoyltransferase ( SPT ) , the enzyme catalyzing the initial step in the biosynthesis of sphingolipids , comprises two different subunits , LCB 1 and LCB 2 . ^^^ Interestingly , there was far less LCB 2 in LY B cells than in wild type cells , and the amount of LCB 2 in LY B cells was restored to the wild type level by transfection with LCB 1 cDNA . ^^^ In addition , overproduction of the LCB 2 subunit required co overproduction of the LCB 1 subunit . ^^^ These results indicated that the LCB 1 subunit is most likely an integral protein having a single transmembrane domain with a lumenal orientation of its N terminus in the endoplasmic reticulum and that the LCB 1 subunit is indispensable for the maintenance of the LCB 2 subunit in mammalian cells . . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| PDT had no effect on either mRNA amounts of the SPT subunits LCB 1 and LCB 2 , LCB 1 protein expression , or SPT activity in Jurkat cells . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| In contrast to Spt 1 , the Spt 2 gene is not expressed at detectable levels in the lacrimal gland . . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Two new yeast strains ( SPT 1 and SPT 2 ) were isolated and immobilized on glassy carbon electrodes to form microbial biosensors for estimation of biochemical oxygen demand ( BOD ) . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| After isolation from a pulp mill wastewater treatment facility , two yeast strains , designated SPT 1 and SPT 2 , were characterized and used in the development of mediated biochemical oxygen demand ( BOD ) biosensors for wastewater . 18S rRNA gene sequence analysis revealed a one nucleotide difference between the sequence of SPT 1 and those of Candida sojae and Candida viswanthii . ^^^ Neighbor joining dendrograms indicated that SPT 1 clustered with several Candida spp . , and that SPT 2 clustered with Starmera spp . , albeit as a very deep branch . ^^^ Physiological tests , microscopic observations , and fatty acid analysis confirmed that SPT 1 and SPT 2 are novel yeast strains . ^^^ The lower detection limits of SPT 1 and SPT 2 based K3Fe ( CN ) 6 mediated biosensors for a pulp mill effluent were 2 and 1 mg BOD / L , respectively . ^^^ Reliability and sensitivity of the SPT 1 and SPT 2 based biosensors were good , but varied with the wastewater . . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| This integral microsomal membrane protein consists of at least two subunits , SPT 1 and SPT 2 . ^^^ In this study we analyzed the expression of SPT 1 and SPT 2 in normal human tissues . ^^^ Strong SPT 1 and SPT 2 expression was observed in pyramidal neurons in the brain , in colon epithelium , and in mucosal macrophages . ^^^ However , SPT 2 expression was more prominent than SPT 1 in the colon mucosal macrophages , the adrenomedullary chromaffin cells and endothelium , and in the uterine endothelium . ^^^ SPT 2 was localized in both nuclei and cytoplasm of the adrenomedullary chromaffin cells , whereas SPT 1 was primarily cytoplasmic . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| We report increased expression of the two subunits , SPT 1 and SPT 2 , in the proliferating cells in these models . ^^^ In addition , we observed SPT 1 and SPT 2 immunoreactivity in reactive stromal fibroblasts surrounding the carcinoma cells of some of the tumors . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Serine palmitoyltransferase ( SPT ) is a heterodimeric molecule made up of two subunits , SPTLC 1 and SPTLC 2 . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| Deletion of the comparable domain of the mammalian protein SPTLC 1 also had little effect on its function , demonstrating that this region is not required for membrane localization or heterodimerization with SPTLC 2 . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| To evaluate in vivo SPT activity and its role in sphingolipid metabolism , we applied homologous recombination to embryonic stem cells , producing mice with long chain base 1 ( Sptlc 1 ) and long chain base 2 ( Sptlc 2 ) , two subunits of SPT , gene deficiency . ^^^ Homozygous Sptlc 11 and Sptlc 2 mice are embryonic lethal , whereas heterozygous versions of both animals ( Sptlc 1 ( + / ) , Sptlc 2 ( + / ) ) are healthy . ^^^ These results indicated that both Sptlc 1 and Sptlc 2 interactions are necessary for SPT activity in vivo , and that SPT activity directly influences plasma sphingolipid levels . ^^^ |
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| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15269 and O15270 |
Pubmed |
SVM Score :0.0 |
| NA |
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