| Interacting proteins: O15151 and Q01094 |
Pubmed |
SVM Score :0.61889729 |
| While an in vivo association of MdmX with either E2F1 or DP 1 was not observed , a slight reduction in DP 1 and an increased cytoplasmic localization of E2F1 were seen in cells overexpressing MdmX . 0.61889729^^^ |
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| Interacting proteins: O15151 and Q01094 |
Pubmed |
SVM Score :0.0 |
| In these studies we find that coexpression of MDMX with E2F 1 results in degradation of the MDMX protein . ^^^ |
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| Interacting proteins: O15151 and Q01094 |
Pubmed |
SVM Score :0.0 |
| The E2F 1 transcription factor is negatively regulated by its interaction with the MDMX protein . ^^^ Further , when expressed in vivo or in vitro the MDMX protein migrates as two isoforms on SDS PAGE , the faster migrating isoform having the stronger affinity for the E2F 1 proteins . ^^^ These data indicate that the MDMX protein is capable of associating with E2F 1 and negatively regulating its DNA binding ability . . ^^^ Here we identify the MDMX oncoprotein as an E2F 1 binding factor , from a yeast two hybrid screen using a portion of the E2F 1 protein as `` bait . ' ' We demonstrate that the region within MDMX needed for the E2F 1 : MDMX interaction is located in the central part of the protein , C terminal of the p 53 binding domain . ^^^ Expression of MDMX along with E2F 1 and Dp 1 in Saos 2 cells reduces the ability of E2F 1 to bind to its consensus DNA sequence , without altering E2F 1 protein levels . ^^^ |
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| Interacting proteins: O15151 and Q01094 |
Pubmed |
SVM Score :0.0 |
| E2F1 induces cell death , calpain activation , and MDMX degradation in a transcription independent manner implicating a novel role for E2F1 in neuronal loss in SIV encephalitis . ^^^ Recently , an E2F1 mutant lacking the DNA binding domain , E2F1 ( 180 437 ) , has been implicated in degradation of MDMX and MDM 2 proteins via lysosomal proteases . ^^^ E2F1 ( 180 437 ) elevates the activity of the calcium activated protease , calpain , which is required for E2F1 induced proteolysis of MDMX and E2F1 induced cell loss . ^^^ To determine if E2F1 could be activating proteolysis via calpains in neurodegeneration , we examined MDMX immunofluorescence in simian immunodeficiency virus encephalitis ( SIVE ) . ^^^ We found a reciprocal relationship between E2F1 and MDMX staining : in SIVE where E2F1 immunostaining is increased , MDMX is decreased , while in controls where E2F1 immunostaining is low , MDMX is high . ^^^ |
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