| PAN 2 also suppressed NF kappaB induction resulting from overexpression of several adapter proteins and protein kinases involved in the TNF or IL 1 receptor signal transduction , including TRAF 2 , TRAF 6 , RIP , IRAK 2 , and NF kappaB inducing kinase as well as the IkappaB kinases IKKalpha and IKKbeta . ^^^ PAN 2 also inhibited the cytokine mediated activation of IKKalpha and IKKbeta as measured by in vitro kinase assays . ^^^ Furthermore , PAN 2 association with IKKalpha was demonstrated by co immunoprecipitation assays , suggesting a direct effect on the IKK complex . ^^^ |