| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.9789308 |
| Genetic interactions between Mot 1 and TBP indicate that their functions are interlinked in vivo . 0.9789308^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.72279671 |
| Mot 1 stably associates with the TATA binding protein ( TBP ) , and it can dissociate TBP from DNA in an ATP dependent manner . 0.72279671^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Not only does TAF 172 activate pol 3 transcription , but it also inhibits the binding of TBP to the TATA box , thereby repressing pol 2 transcription . ^^^ The TBP TAF 172 TAF L complex can replace TFIIIB both in transcription reactions reconstituted with TFIIIC and in template commitment assays . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Here we describe the cloning and biochemical characterization of an abundant human TBP associated factor ( TAF 172 ) which is homologous to the yeast Mot 1 protein and a member of the larger Snf2 / Swi2 family of DNA targeted ATPases . ^^^ Like Mot 1 , TAF 172 binds to the conserved core of TBP and uses the energy of ATP hydrolysis to dissociate TBP from DNA ( ADI activity ) . ^^^ Interestingly , ATP also causes TAF 172 to dissociate from TBP , which has not been previously observed with Mot 1 . ^^^ Unlike Mot 1 , TAF 172 requires both TBP and DNA for maximal ( approximately 100 fold ) ATPase activation . ^^^ TAF 172 inhibits TBP driven RNA polymerase 2 and 3 transcription but does not appear to affect transcription driven by TBP TAF complexes . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| The transcriptional regulators of alpha 1 fetoprotein transcription factor , TBP associated factor 172 ( TAF 172 ) and the human zinc finger protein 5 ( ZFP 5 ) were downregulated . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Genetic identification of Mot1p as a repressor of pol 2 transcription was supported by findings that Mot1p and BTAF 1 could dissociate TBP from TATA DNA complexes using the energy of ATP hydrolysis . ^^^ Recent data have revealed new aspects of BTAF 1 and Mot1p as positive regulators of TBP function in the pol 2 system and have described new observations relating to their molecular mechanism of action . ^^^ We review these data in the context of previous findings with particular attention paid to how human BTAF 1 and Mot1p may dynamically regulate TBP function on pol 2 promoters in cells . . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| BTAF 1 ( formerly named TAF ( 2 ) 170 / TAF 172 ) is an essential , evolutionarily conserved member of the SNF 2 like family of ATPase proteins and together with TATA binding protein ( TBP ) forms the B TFIID complex . ^^^ BTAF 1 has been proposed to play a key role in the dynamic regulation of TBP function in RNA polymerase 2 transcription . ^^^ Immunolabeling experiments localize the extreme carboxyl terminus of BTAF 1 within the 170 kDa domain , whereas the amino terminus and TBP co localize to the end of the protruding thumb . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| The BTAF 1 protein ( formerly known as TAF ( 2 ) 170 / TAF 172 and the human ortholog of Saccharomyces cerevisiae Mot1p ) and the NC 2 complex composed of NC2alpha ( DRAP 1 ) and NC2beta ( Dr 1 ) are able to bind to TBP directly and regulate RNA polymerase 2 transcription both positively and negatively . ^^^ In contrast , the NC2beta subunit is not able to associate with BTAF 1 and seems to interfere with the BTAF 1 TBP interaction . ^^^ Addition of NC2alpha or the NC 2 complex can stimulate the ability of BTAF 1 to interact with TBP . ^^^ Together , our results constitute the first evidence of the physical cooperation between BTAF 1 and NC2alpha in TBP regulation and provide a framework to understand transcription functions of NC2alpha and NC2beta in vivo . . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Mutational analysis of BTAF 1 TBP interaction : BTAF 1 can rescue DNA binding defective TBP mutants . ^^^ The BTAF 1 transcription factor interacts with TATA binding protein ( TBP ) to form the B TFIID complex , which is involved in RNA polymerase 2 transcription . ^^^ Here , we present an extensive mapping study of TBP residues involved in BTAF 1 interaction . ^^^ This shows that residues in the concave , DNA binding surface of TBP are important for BTAF 1 binding . ^^^ In addition , BTAF 1 interacts with residues in helix 2 on the convex side of TBP as assayed in protein protein and in DNA binding assays . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| In addition , other cofactors , such as TAF 1 , BTAF 1 , and negative cofactor 2 ( NC 2 ) , can also modulate TBP or TFIID binding to the core promoter . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Yeast Taf 170 is encoded by MOT 1 and exists in a TATA box binding protein ( TBP ) TBP associated factor complex distinct from transcription factor IID . ^^^ We describe in this report a series of experiments that demonstrate that the protein encoded by MOT 1 is a bona fide yeast TAF and that Taf 170 forms a separate complex with TBP distinct from the RNA polymerase 2 specific multisubunit transcription factor IID TBP TAF complex . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Third , not mutations suppressed toxicity due to overexpression of TBP in mot 1 1 mutants . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| In this way , we have identified a mutation in MOT 1 , which encodes an ATP dependent inhibitor of TBP binding to TATA boxes : Previous analyses suggested that Mot 1 causes repression in vivo . ^^^ We believe that , taken together , these data suggest that Spt 3 , Mot 1 , and TFIIA cooperate to regulate TBP DNA interactions , perhaps at the level of TATA box selection in vivo . . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| BUR 3 is identical to MOT 1 , a previously characterized essential gene that encodes an ATP dependent inhibitor of the TATA box binding protein . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| MOT 1 functions by removing TATA binding protein ( TBP ) from DNA , and as a consequence , MOT 1 can regulate transcription both in vitro and in vivo . ^^^ The results indicate that MOT 1 is targeted to TBP both in vitro and in vivo via amino acids in its nonconserved N terminus . ^^^ The conserved C terminal ATPase of MOT 1 appears to contribute to TBP DNA complex recognition in the absence of ATP , but it appears to function primarily during the actual ATP dependent dissociation reaction . ^^^ Chimeric proteins in which homologous portions of SNF2 / SWI2 have been substituted for the MOT 1 ATPase can bind to TBP DNA complexes but fail to dissociate these complexes in the presence of ATP , suggesting that the specificity of action of MOT 1 is also conferred by the C terminal ATPase . ^^^ ATPase assays demonstrate that the MOT 1 ATPase is activated by TBP . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Spt 3 and Mot 1 are two transcription factors of Saccharomyces cerevisiae that are thought to act in a related fashion to control the function of TATA binding protein ( TBP ) . ^^^ Current models suggest that while Spt 3 and Mot 1 do not directly interact , they do function in a related fashion to stabilize the TBP TATA interaction at particular promoters . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| MOT 1 is an essential Saccharomyces cerevisiae transcription factor that uses ATP to dissociate TATA binding protein ( TBP ) from DNA . ^^^ The results demonstrate that , following ATP dependent TBP DNA dissociation , MOT 1 dissociates rapidly from the DNA by a mechanism that does not require a DNA end . ^^^ These results support a model in which MOT 1 drives TBP DNA dissociation by a mechanism that involves a transient , ATP dependent interaction with TBP DNA which does not involve ATP dependent DNA tracking . . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| MOT 1 is an ATPase which can dissociate TATA binding protein ( TBP ) DNA complexes in a reaction requiring ATP hydrolysis . ^^^ Quantitation of MOT 1 revealed that it is an abundant protein , with nuclear extracts from wild type cells containing a molar excess of MOT 1 over TBP . ^^^ This activation by MOT 1 is detectable with amounts of MOT 1 that are approximately stoichiometric to TBP . ^^^ These results suggest that MOT 1 might activate transcription via an indirect mechanism in which limiting TBP can be liberated from nonpromoter sites for use at promoters . ^^^ In support of this idea , excess nonpromoter DNA sequesters TBP and represses transcription , but this effect can be reversed by addition of MOT 1 . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Using mutant yeast strains , we show that Mot 1 prevents the binding of TBP to inactive promoters and that activator mediated stimulation of TBP binding requires additional GTFs , including TFIIB and Srb 4 . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Mot 1 is an essential regulator of RNA polymerase 2 dependent transcription in vivo and dissociates TATA box binding protein ( TBP ) DNA complexes in vitro . ^^^ Correspondingly , at moderate salt concentration , Mot 1 ATPase ( but not Mot1C ) was stimulated > / =10 fold by yeast TBP , suggesting that interaction with TBP relieves a conformational constraint in Mot 1 . ^^^ Double or single stranded TATA containing DNA did not affect ATPase activity of Mot 1 or Mot1C , with or without TBP . ^^^ Mot 1 catalyzed dissociation of TBP from DNA was not prevented by a psoralen cross link positioned immediately preceding the TATA sequence . ^^^ Thus , Mot 1 most likely promotes release of TBP from TATA containing DNA by causing a structural change in TBP itself , rather than by strand unwinding . . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Once TBP is bound to DNA , factors such as TAF ( 2 ) 250 and Mot 1 induce TBP to dissociate , while other factors such as NC 2 and the NOT complex convert the TBP / DNA complex into an inactive state . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| High affinity interaction of yeast transcriptional regulator , Mot 1 , with TATA box binding protein ( TBP ) . ^^^ Yeast Mot 1 , an essential ATP dependent regulator of basal transcription , removes TATA box binding protein ( TBP ) from TATA sites in vitro . ^^^ Complexes of Mot 1 and Spt 15 ( yeast TBP ) , radiolabeled in vitro , were immunoprecipitated with anti TBP ( or anti Mot 1 ) antibodies in the absence of DNA , showing Mot 1 binds TBP in solution . ^^^ Mot 1 N terminal deletions ( residues 25 801 ) abolished TBP binding , whereas C terminal ATPase domain deletions ( residues 802 1867 ) did not . ^^^ Correspondingly , TBP variants lacking solvent exposed positive charge did not bind Mot 1 , whereas a mutant lacking positive charge within the DNA binding groove bound Mot 1 . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| MOT 1 catalyzed TBP DNA disruption : uncoupling DNA conformational change and role of upstream DNA . ^^^ Here we examine the mechanism of action of MOT 1 , a yeast SNF2 / SWI2 related ATPase that uses ATP hydrolysis to remove TATA binding protein ( TBP ) from DNA . ^^^ Remarkably , MOT 1 catalyzed disruption of TBP DNA does not appear to require DNA strand separation , DNA bending or twisting of the DNA helix . ^^^ Combining these results with proposed similarities between SNF2 / SWI2 ATPases and helicases , we suggest that MOT 1 uses ATP hydrolysis to translocate along the handle and thereby disrupt interactions between TBP and DNA . . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| TBP recruitment was compromised in two different mot 1 mutant strains , but was only moderately affected in a taf 1 Ts strain . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Mot 1 is an essential Snf2 / Swi2 related Saccharomyces cerevisiae protein that binds the TATA binding protein ( TBP ) and removes TBP from DNA using ATP hydrolysis . ^^^ Mot 1 catalysis of TBP . ^^^ Mot 1 and NC 2 can co occupy TBP . ^^^ Residues on the DNA binding surface of TBP are important for Mot 1 binding and the Mot1 . ^^^ A model for Mot 1 action is proposed in which ATP hydrolysis causes the Mot 1 N terminus to displace the TATA box , leading to ejection of Mot 1 and TBP from DNA . . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Spt 3 and Mot 1 cooperate in nucleosome remodeling independently of TBP recruitment . ^^^ In an effort to explore how the Spt 3 function was elicited , we found that Mot 1 , an ATPase of the Snf 2 family that genetically interacts with Spt 3 , was also required for nucleosome remodeling independently of TBP recruitment . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Cellular stress alters the transcriptional properties of promoter bound Mot 1 TBP complexes . ^^^ As determined by sequential chromatin immunoprecipitation , Mot 1 co occupies promoters with TBP , but not with TFIIB , TFIIA , or Pol 2 when cells are grown in normal conditions . ^^^ This strongly suggests that the Mot 1 TBP complex is transcriptionally inactive , and hence is in dynamic equilibrium with transcriptionally active forms of TBP . ^^^ Thus , Mot 1 TBP complexes can exist in active and inactive forms that are regulated by environmental stress . . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Mot 1 is an essential Snf2 / Swi2 related ATPase and TATA binding protein ( TBP ) associated factor ( TAF ) . ^^^ In vitro , Mot 1 utilizes ATP hydrolysis to disrupt TBP DNA complexes , but the relationship of this activity to Mot 1 ' s in vivo function is unclear . ^^^ We find that the Mot 1 repressed HSP 26 and INO 1 promoters are both regulated by TBP recruitment ; inactivation of Mot 1 leads to increased PIC formation coincident with derepression of transcription . ^^^ For the Mot 1 activated genes BNA 1 and URA 1 , inactivation of Mot 1 also leads , remarkably , to increased TBP binding to the promoters , despite the fact that transcription of these genes is obliterated in mot 1 cells . ^^^ These results suggest that Mot 1 mediated displacement of TBP underlies its mechanism of repression and activation at these genes . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| In this report we examine the genetic relationship between Nhp 6 and Gcn 5 with the Mot 1 and Ccr 4 Not complexes , both of which have been implicated in regulating DNA binding by TBP . ^^^ Combining spt 15 point mutations ( in TBP ) with either mot 1 or ccr 4 also results in either a growth defect or lethality . ^^^ Chromatin immunoprecipitation experiments show decreased binding of TBP to promoters in mot 1 mutants and a further decrease when combined with either nhp6ab or gcn 5 mutations . . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Among the genes we cloned is MOT 1 , encoding a repressor that inhibits TBP binding to the promoter , thus linking glucose repression with TBP access to chromatin . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| Mot 1 is a conserved Snf2 / Swi2 related transcriptional regulator that uses ATP hydrolysis to displace TATA binding protein ( TBP ) from DNA . ^^^ Several models of the enzymatic mechanism have been proposed , including Mot 1 catalyzed distortion of TBP structure , competition between Mot 1 and DNA for the TBP DNA binding surface , and ATP driven translocation of Mot 1 along DNA . ^^^ Mot 1 forms an asymmetric complex with the TBP core domain ( TBPc ) DNA complex , contacting DNA both upstream and within the major groove of the TATA Box . ^^^ |
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| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14981 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
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