| We examined copy number changes with respect to six candidate genes , E2F1 ( 20q11 . 2 ) , TGIF 2 ( 20q11 . 2 ) , AIB 1 ( 20q12 ) , PTPN 1 ( 20q13 . 1 ) , ZNF 217 ( 20q13 . 2 ) , and BTAK ( 20q13 ) , and then measured transcription levels of each candidate in 18 OC cell lines . ^^^ Three distinct cores of amplification were identified : 20q11 . 2 , harboring E2F1 and TGIF 2 ( region 1 ; 1 of 18 cell lines , 5 . 6 % ) ; 20q13 . 1 , harboring PTPN 1 ( region 2 ; 5 lines , 27 . 8 % ) ; and 20q13 . 2 , harboring ZNF 217 and BTAK ( region 3 ; 6 lines , 33 . 3 % ) . ^^^ |
| The aim of the study presented here was to analyze the chromosomal aberrations and to determine the numbers of copies of AIB 1 , BTAK , DcR 3 and E2F1 as putative target genes on chromosome 20q as well as their expression and relation to clinicopathological features in 41 primary tumors of esophageal squamous cell carcinoma . ^^^ AIB 1 amplification was observed in 4 . 9 % ( 2 / 41 ) , BTAK amplification in 9 . 8 % ( 4 / 41 ) , DcR 3 amplification was in 4 . 9 % ( 2 / 41 ) , and E2F1 amplification in 7 . 3 % ( 3 / 41 ) . ^^^ The survival of patients with BTAK or E2F1 amplification was significantly lower than that of patients without these abnormalities . ^^^ Our results suggest that amplification of BTAK or E2F1 are likely to lead to an increase in the number of malignant phenotypes of esophageal squamous cell carcinoma and that these aberrations can be expected to be useful as markers of poor prognosis . . ^^^ |