| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.5390324 |
| In this work , we show that STAM 1 and STAM 2 , as well as an endocytic regulator protein , Eps 15 , can be co immunoprecipitated with Hrs both from membrane and cytosolic fractions and that recombinant Hrs , STAM1 / STAM2 , and Eps 15 form a ternary complex . 0.5390324^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.5108081 |
| Members of the STAM family of proteins , STAM 1 and STAM 2 , are associated with Hrs through their coiled coil regions . 0.5108081^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| The DDP STAM 1 interaction requires a coiled coil region in STAM 1 that overlaps with the immunoreceptor tyrosine based activation motif ( ITAM ) , a region previously shown to be important for interaction with Jak2 / 3 and hepatocyte growth factor regulated tyrosine kinase substrate ( Hrs ) . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| We found that UIM peptides from several proteins involved in endocytosis and vacuolar protein sorting including Hrs , Vps27p , Stam 1 , and Eps 15 bound specifically , but with modest affinity ( Kd = 0 . 1 1 mm ) , to free ubiquitin . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| Signal transducing adaptor molecule ( STAM ) proteins , STAM 1 and STAM 2 , are associated with hepatocyte growth factor regulated substrate ( Hrs ) but their function remains unknown . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| We also demonstrate that ligand mediated epidermal growth factor receptor ( EGFR ) degradation is partially but not completely impaired in both Hrs ( / ) and STAM 1 ( / ) STAM 2 ( / ) mouse embryonic fibroblasts . ^^^ Furthermore , endosome swelling is seen in both Hrs ( / ) and STAM 1 ( / ) STAM 2 ( / ) cells . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| In the hrs ( / ) cells only trace amounts of STAM 1 and STAM 2 were detected . ^^^ Introduction of wild type Hrs or an Hrs mutant with an intact STAM binding domain ( Hrs dFYVE ) fully restored STAM 1 and STAM 2 expression , whereas mutants with no STAM binding ability ( Hrs dC 2 , Hrs dM ) failed to express the STAMs . ^^^ Revertant Hrs expression in hrs ( / ) cells not only led to the accumulation of ubiquitinated proteins , including intracytoplasmic vesicles , but also restored STAM 1 levels in early endosomes and eliminated the enlarged endosome phenotype caused by the absence of Hrs . ^^^ These results suggest that Hrs is a master molecule that controls in part the degradation of STAM 1 and the accumulation of ubiquitinated proteins . . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| Hrs interacts with a number of proteins , including SNAP 25 and STAM 1 , forming a complex that binds ubiquitin moieties . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| The 110 kDa molecule was found to be a human counterpart of mouse Hrs ( hepatocyte growth factor regulated tyrosine kinase substrate ) and to be associated with STAM . ^^^ Hrs is associated with STAM , a signal transducing adaptor molecule . ^^^ The mutual association sites of Hrs and STAM include highly conserved coiled coil sequences , suggesting that their association is mediated by the coiled coil structures . ^^^ Exogenous introduction of the wild type Hrs significantly suppressed DNA synthesis upon stimulation with IL 2 and granulocyte macrophage colony stimulating factor , while the Hrs mutant deleted of the STAM binding site lost such suppressive ability . ^^^ These results suggest that Hrs counteracts the STAM function which is critical for cell growth signaling mediated by the cytokines . . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| The N terminal domain of the TOM 1 protein shares similarity with HGS ( hepatocyte growth factor regulated tyrosine kinase substrate ) and STAM ( signal transducing adaptor molecule ) , which are associated with vesicular trafficking at the endosome . ^^^ TOM 1 genes map to human chromosome 22q13 . 1 and mouse chromosome 8C1 and encode proteins similar to the endosomal proteins HGS and STAM . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| Most importantly , in addition to several known tyrosine phosphorylation sites , we have identified five novel sites on SHIP 2 , Hrs , Cbl , STAM , and STAM 2 , most of which were not predicted to be phosphorylated . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| The basic domain architecture is comprised of an N terminal Vps 27 , Hrs and STAM homology domain , a ubiquitin interacting motif and a central Src homology 3 domain . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| The VHS ( Vps27p , Hrs and STAM ) domains of the GGA proteins are responsible for the highly specific recognition of these acidic cluster dileucine signals . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| The VHS ( Vps 27 , Hrs and STAM ) domain is a 140 residue long domain present in the very NH 2 terminus of at least 60 proteins . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| These proteins have modular structures with an N terminal VHS ( VPS 27 , Hrs , and STAM ) domain followed by a GAT ( GGA and TOM 1 ) domain , a connecting hinge segment , and a C terminal GAE ( gamma adaptin ear ) domain . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| HRS is a powerful regulator of receptor tyrosine kinase trafficking to the degradation pathway and HRS also binds STAM . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| The VHS ( VPS 27 , Hrs , and STAM ) domain of GGA binds with the signal peptides . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| TOM 1 is a protein closely related to the VHS ( VPS 27 , Hrs , and STAM ) domain containing GGA family . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| We described previously that the cytosolic domain of memapsin 2 contains an acid cluster dileucine motif ( ACDL ) that binds the VHS ( Vps 27 , Hrs , and STAM ) domain of Golgi localized gamma ear containing ARF binding ( GGA ) proteins ( He , X . , Zhu , G . , Koelsch , G . , Rodgers , K . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| Lsb5p has a similar structure to the GGA [ Golgi localized , gamma ear containing , Arf ( ADP ribosylation factor ) binding ] family of proteins with an N terminal VHS [ Vps27p ( vacuolar protein sorting protein 27 ) , Hrs , Stam ] domain and a GAT ( GGA and Tom 1 ) domain . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| The Hrs / STAM complex in the downregulation of receptor tyrosine kinases . ^^^ Hrs and STAM are proteins that form a complex on the endosomal membrane . ^^^ Recent studies have shown that the Hrs / STAM complex binds ubiquitin moieties and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting / trafficking processes . . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| Using the techniques of immunoadsorption as well as glutathione S transferase pull down assay we found that Glut 4 vesicles ( but not Glut 4 per se ) interact with GGA via the Vps 27 , Hrs , and STAM ( VHS ) domain . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| Hrs ( hepatocyte growth factor regulated tyrosine kinase substrate ) and STAM ( signal transducing adaptor molecule ) form a heterodimeric complex that associates with endosomal membranes and is tyrosine phosphorylated in response to a variety of growth factors including EGF ( epidermal growth factor ) , HGF ( hepatocyte growth factor ) and PDGF ( platelet derived growth factor ) . ^^^ Growth factors induce differential phosphorylation profiles of the Hrs STAM complex : a common node in signalling networks with signal specific properties . ^^^ Hrs ( hepatocyte growth factor regulated tyrosine kinase substrate ) and STAM ( signal transducing adaptor molecule ) form a heterodimeric complex that associates with endosomal membranes and is tyrosine phosphorylated in response to a variety of growth factors including EGF ( epidermal growth factor ) , HGF ( hepatocyte growth factor ) and PDGF ( platelet derived growth factor ) . ^^^ Phosphorylation of the Hrs STAM complex requires receptor endocytosis . ^^^ Despite this conservation , kinase inhibitor profiles using PP 1 ( 4 amino 5 ( 4 methylphenyl ) 7 ( t butyl ) pyrazolo [ 3 , 4 d ] pyrimidine ) and SU 6656 indicate that distinct non receptor tyrosine kinases couple EGF , HGF and PDGF stimulation with the tyrosine phosphorylation of the Hrs STAM complex . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| It directly binds to the SH 3 domain of STAM , which is constitutively associated with Hrs , a component of clathrin coated structures on endosomes . ^^^ AMSH and STAM , like Hrs , both bind directly to clathrin . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| Overexpression of a truncated , GFP tagged GGA , VHS ( Vps 27 , Hrs , Stam ) GAT ( GGA and target of myb ( TOM ) ) GFP led to retention of MPR , VAMP 4 , and syntaxin 6 in mature SGs ( MSGs ) , suggesting that CCV budding from ISGs is inhibited by the SG localizing VHS GAT GFP . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| Endosomal sorting of ubiquitinated membrane proteins for trafficking to lysosomes is executed by a complex of two ubiquitin binding proteins , Hrs and STAM , that localizes on a microdomain of early endosomes with a flat clathrin coat . ^^^ |
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| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92783 and O14964 |
Pubmed |
SVM Score :0.0 |
| NA |
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