| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.50383584 |
| Hbp was co immunoprecipitated with Hrs , and its intracellular localization was similar to that of Hrs . 0.50383584^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| Most importantly , in addition to several known tyrosine phosphorylation sites , we have identified five novel sites on SHIP 2 , Hrs , Cbl , STAM , and STAM 2 , most of which were not predicted to be phosphorylated . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| STAM 1 and STAM 2 , which have been identified as regulators of receptor signaling and trafficking , interact directly with Hrs , which mediates the endocytic sorting of ubiquitinated membrane proteins . ^^^ In this work , we show that STAM 1 and STAM 2 , as well as an endocytic regulator protein , Eps 15 , can be co immunoprecipitated with Hrs both from membrane and cytosolic fractions and that recombinant Hrs , STAM1 / STAM2 , and Eps 15 form a ternary complex . ^^^ We find that overexpression of Hrs causes a strong recruitment of STAM 2 to endosome membranes . ^^^ Moreover , STAM 2 , like Hrs and Eps 15 , binds ubiquitin , and Hrs , STAM 2 , and Eps 15 colocalize with ubiquitinated proteins in clathrin containing endosomal microdomains . ^^^ The localization of Hrs , STAM 2 , Eps 15 , and clathrin to endosome membranes is controlled by the AAA ATPase mVps 4 , which has been implicated in multivesicular body formation . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| Signal transducing adaptor molecule ( STAM ) proteins , STAM 1 and STAM 2 , are associated with hepatocyte growth factor regulated substrate ( Hrs ) but their function remains unknown . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| We also demonstrate that ligand mediated epidermal growth factor receptor ( EGFR ) degradation is partially but not completely impaired in both Hrs ( / ) and STAM 1 ( / ) STAM 2 ( / ) mouse embryonic fibroblasts . ^^^ Furthermore , endosome swelling is seen in both Hrs ( / ) and STAM 1 ( / ) STAM 2 ( / ) cells . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| Members of the STAM family of proteins , STAM 1 and STAM 2 , are associated with Hrs through their coiled coil regions . ^^^ A STAM 2 mutant that lacks the coiled coil region and does not bind Hrs , in contrast , mislocalized to the cytoplasm . ^^^ Deletion of a region located N terminal to the coiled coil region and conserved among STAM proteins also severely affected Hrs binding and the endosomal localization of STAM 2 , suggesting that this region is also involved in these activities . ^^^ Depletion of endogenous Hrs by RNA interference similarly caused the mislocalization of exogenously expressed STAM 2 to the cytoplasm . ^^^ Finally , STAM 2 mutants lacking the Hrs binding activity were defective in causing the enlargement of early endosomes , accumulating ubiquitinated proteins on this aberrant organelle , and inhibiting the degradation of ligand activated epidermal growth factor receptors , suggesting that the association with Hrs is a prerequisite for STAM function . . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| In the hrs ( / ) cells only trace amounts of STAM 1 and STAM 2 were detected . ^^^ Introduction of wild type Hrs or an Hrs mutant with an intact STAM binding domain ( Hrs dFYVE ) fully restored STAM 1 and STAM 2 expression , whereas mutants with no STAM binding ability ( Hrs dC 2 , Hrs dM ) failed to express the STAMs . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| MEASUREMENTS AND MAIN RESULTS : MORTALITY RATE : Survival was increased in mice pretreated intraperitoneally 24 hrs before CLP with 10 microg or 100 microg of HBP without cefoxitin ( p = . 01 , Cox Mantel log rank test ) . ^^^ Compared with control animals , survival was increased significantly ( from 5 % to 47 % , p = . 014 ) in mice that received cefoxitin and 50 microg ip HBP immediately after CLP , followed by continuous administration of HBP ( 12 microg / 24 hrs ) . ^^^ CHEMOTAXIS : Cytospin preparations from peritoneal exudate cells ( PECs ) 48 hrs after administration of 10 microg and 100 microg ip HBP demonstrated a 1 . 7 fold increase in the total number of macrophages compared with carrier control ( p < . 05 ) . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| Hrs binding protein ( Hbp ) is a Src homology 3 ( SH 3 ) domain containing protein that tightly associates with Hrs . ^^^ Hbp together with Hrs is thought to play a regulatory role in endocytic trafficking of growth factor receptor complexes through early endosomes . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| Hrs binding protein ( Hbp ) tightly associated with Hrs is thought to play a regulatory role in vesicular trafficking during endocytosis and exocytosis . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| Hrs and hbp : possible regulators of endocytosis and exocytosis . ^^^ Hrs and Hbp , two tightly associated proteins in eukaryotic cells , have been implicated in these cellular processes . ^^^ These observations suggest an important role for the Hrs / Hbp protein complex in vesicular trafficking during endocytosis and exocytosis . . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| Two other UIM containing endocytic proteins , Hrs and Hbp , also interact with ubiquilin in a UIM dependent manner , whereas epsin does not . ^^^ |
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| Interacting proteins: O75886 and O14964 |
Pubmed |
SVM Score :0.0 |
| NA |
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