Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Human gastric cancer with expression of CEA , ER , E 2 , EGF and EGF R ] . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Overexpression of EGFR did not result in loss of ER expression . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR status was inversely correlated with estrogen receptor ( ER ) status . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We failed to detect expression of the ErbB ligand , ER . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
This xenograft was ER , PR , Her 2 / neu negative and p 53 , EGFR positive . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
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Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.67867753
Epiregulin binds to epidermal growth factor receptor and ErbB 4 and induces tyrosine phosphorylation of epidermal growth factor receptor , ErbB 2 , ErbB 3 and ErbB 4 . 0.67867753^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.73999043
To determine whether an increase in EGFR expression might alter the estrogen responsiveness of an ER positive human breast cancer cell line , ZR 75 1 cells were cotransfected with a plasmid containing the full length cDNA for the human EGFR under the transcriptional control of the Harvey murine sarcoma virus ( HaMSV ) long terminal repeat ( LTR ) and with a pSV2neo plasmid . 0.73999043^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :1.1345112
ARAC was quantified by Thompson and Siiterii ' s method , EGFR was quantified with a two point assay method using radioactive iodine ( 125I ) EGF as ligand , and ER was measured by the Scatchard method using 3H E 2 . 1.1345112^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.54735383
An inverse relationship between EGF r and ER was found ( chi 2= 7 . 81 , P less than 0 . 01 ) with a higher incidence of EGF r positive tumours in the metastatic group ( chi 2 = 14 . 51 , P less than 0 . 001 ) . 0.54735383^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.51864192
The well established inverse relationship between EGFR ( as measured by this assay ) and oestrogen receptor ( ER ) was seen ( chi 2 = 24 . 9 , P < 0 . 0001 ) . 0.51864192^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.85467488
A strong positive association was detected between ER and PgR , and EGFR was observed to present a weak positive correlation with Ki 67 and ER . 0.85467488^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.54103553
On the other hand , the level of EGFR ligand ( s ) was positively associated with ER levels and was three fold higher in HD / HR tumors than in HI tumors . 0.54103553^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.52487863
Molecular cross talk between the ER and HER 2 pathways was increased in the MCF 7 / HER 2 cells compared with MCF 7 cells , with cross phosphorylation and activation of both the ER and the EGFR / HER2 receptors , the signaling molecules AKT and ERK 1 , 2 mitogen activated protein kinase ( MAPK ) , and AIB 1 itself with both estrogen and tamoxifen treatment . 0.52487863^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.98000215
It was demonstrated that the ER specific chaperone , glucose regulated protein 78 ( GRP78 / BiP ) formed a stable complex with EGFR in MNNG treated cell . 0.98000215^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We show here that EPR activates two members of the ErbB family of receptor tyrosine kinases , epidermal growth factor receptor ( EGFR ) and ErbB 4 . ^^^ EPR stimulates higher levels of EGFR phosphorylation than does BTC , whereas BTC stimulates higher levels of ErbB 4 phosphorylation than does EPR . ^^^ Moreover , the EPR and BTC dose response curves show that although EGFR is more sensitive to EPR than is ErbB 4 , ErbB 4 is more sensitive to BTC than is EGFR . ^^^ Finally , ErbB 2 , which is not activated by EPR when expressed on its own , increases the sensitivity of ErbB 4 for activation by EPR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Room temperature continuous wave EPR measurements at 10 band of whole cells of menA and menB interruption mutants show a transient reduction and oxidation of an organic radical with a g value and anisotropy characteristic of a quinone . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epiregulin ( EPR ) is a broad specificity EGF family member that activates ErbB 1 and ErbB 4 homodimers and all possible heterodimeric ErbB complexes . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epiregulin ( EPR ) , a novel member of epidermal growth factor ( EGF ) family , is a ligand for ErbB 1 and ErbB 4 receptors . ^^^ The structural difference in the C terminal domain may provide an explanation for the reduced binding affinity of EPR to the ErbB receptors . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We characterized the expression of epidermal growth factor ( EGF ) like growth factors ( EGF , transforming growth factor alpha ( TGF alpha ) , heparin binding EGF like growth factor ( HB EGF ) , betacellulin ( BTC ) , amphiregulin ( APR ) , epiregulin ( EPR ) and neuregulin ( NRG ) 1 ) and erbB receptors ( EGF receptor ( EGFR ) , erbB2 / neu , erbB 3 and erbB 4 ) in the vaginae of mice treated either neonatally ( 0 4 day ) or as adults ( 55 59 day ) with estrogens . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : We investigated the expression of HB EGF , BTC , EPR and EGF receptor ( EGF R ) in 43 human MFH tissue samples using immunohistochemical techniques . ^^^ RESULTS : Positive immuno reactivity for HB EGF , BTC , EPR and EGF R was identified in 28 ( 65 % ) , 7 ( 16 % ) , 43 ( 100 % ) and 36 ( 84 % ) out of the 43 MFH cases analyzed , respectively . ^^^ Coexpression of HB EGF / EGF R , BTC / EGF R and EPR / EGF R was observed in 25 ( 58 % ) , 6 ( 14 % ) and 36 ( 84 % ) of the MFHs , respectively . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The EGF family includes EGF , transforming growth factor alpha , heparin binding EGF , amphiregulin , beta cellulin , epiregulin , and heregulins , whereas the receptor family ( the erbB genes ) consists of erbB 1 ( EGF receptor , EGF R ) , erbB 2 , erbB 3 , and erbB 4 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epiregulin stimulates proliferation of rabbit gastric cells in primary culture through autophosphorylation of the epidermal growth factor receptor . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Anti EGFR monoclonal antibodies which act as EGF , TGF alpha , HB EGF and BTC antagonists block the binding of epiregulin to EGFR expressing tumours . ^^^ Here , using a panel of anti EGFR receptor ( EGFR ) monoclonal antibodies ( MAbs ) directed against 4 distinct epitopes on the external domain of the receptor , we have investigated the importance of the EGFR in transmitting the biological action of epiregulin . ^^^ We found that MAb ICR 9 , which enhances the binding of EGF , TGF alpha , HB EGF and betacellulin to the EGFR , also increases the binding of 125I epiregulin to a number of EGFR expressing tumour cell lines , including EJ , SKBR 3 , SKOV 3 , MDA MB 468 and HN 5 . ^^^ In addition , anti EGFR MAbs ICR 15 , ICR 16 , ICR 61 , ICR 62 and ICR 80 , which block the binding of 125I EGF to the EGFR , inhibit the binding of 125I epiregulin to these tumour cell lines . ^^^ Like EGF , we found that both the epiregulin induced growth inhibition of HN 5 and MDA MB 468 cells and tyrosine phosphorylation of the 170 kDa EGFR on HN 5 cells are reversed in the presence of anti EGFR MAbs ICR 62 and ICR 80 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epiregulin is a potent pan ErbB ligand that preferentially activates heterodimeric receptor complexes . ^^^ Here we addressed the action of epiregulin , a recently isolated ligand of ErbB 1 . ^^^ By employing a set of factor dependent cell lines engineered to express individual ErbBs or their combinations , we found that epiregulin is the broadest specificity EGF like ligand so far characterized : not only does it stimulate homodimers of both ErbB 1 and ErbB 4 , it also activates all possible heterodimeric ErbB complexes . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In contrast , ErbB 3 showed much more restrictive ligand binding specificity and measurable binding was observed only with heregulin , neuregulin2beta , epiregulin and the synthetic heregulin / egf chimera , biregulin . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In this study , we compared expression levels of messenger RNAs ( mRNAs ) encoding the epidermal growth factor ( EGF ) receptor related receptor tyrosine kinases ( ErbB 1 through 4 ) , the six EGF receptor ligands , EGF , transforming growth factor ( TGF ) alpha , amphiregulin ( ARG ) , HB EGF , betacellulin , and epiregulin , and the related molecule heregulin alpha , in a series of 10 prostate tissue specimens . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Among four EGF receptor ( EGFR ) family members , the EGFR was solely activated by EGF , heparin binding EGF ( HB EGF ) , transforming growth factor alpha ( TGF alpha ) , epiregulin ( ER ) , and betacellulin ( BTC ) , resulting in induction of phenotypic modulation of SMCs . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Each member of the group formed by BTC , heparin binding EGF ( HB EGF ) and epiregulin ( EP ) can interact with both the EGF receptor ( EGFR ) and heregulin receptors ( ErbB 3 and ErbB 4 ) , and are hence called `` bispecific ' ' ligands . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epiregulin induced a more prolonged activation of EGF receptor ( EGFR ) and p42 / 44 mitogen activated protein kinase ( MAPK ) than EGF . ^^^ In vivo , increased production of epiregulin was noted in extracts of the remnant liver obtained 24 h after partial hepatectomy , and EGFR phosphorylation by these extracts was partially inhibited by anti epiregulin antibody . ^^^ Our results showed a more potent hepatocyte proliferative activity for epiregulin compared with EGF in vitro , which depends on prolonged activation of EGFR and p42 / 44 MAPK . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epiregulin is a broad specificity epidermal growth factor family member that activates ErbB 1 and ErbB 4 homodimers and all possible heterodimeric ErbB complexes . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The ErbB 4 protein plays a role as a receptor for the neuregulins , a large group of structurally related molecules and a few other epidermal growth factor ( EGF ) related polypeptides , such as heparin binding EGF , betacellulin and epiregulin . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
To learn about the endogenous response of epidermal growth factor receptor ligands during Fas mediated liver injury we investigated the expression of epidermal growth factor , transforming growth factor alpha , heparin binding epidermal growth factor like growth factor , betacellulin , epiregulin , and amphiregulin in the liver of mice challenged with Fas agonist antibody . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Compressive stress produced a sustained upregulation of the EGFR ligands HB EGF , epiregulin , and amphiregulin , but not transforming growth factor alpha . ^^^ Inhibition with AG 1478 demonstrated that expression of HB EGF , epiregulin , and amphiregulin is dependent on the signaling via the EGFR . ^^^ Immunostaining for epiregulin protein demonstrated increased expression with compression and attenuation with EGFR inhibition . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Finally , in the two cell lines expressing ErbB receptors , we demonstrated that only EGFR / ErbB1 was activated by epiregulin . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We found that normal human lung fibroblasts express transforming growth factor alpha , heparin binding epidermal like growth factor , epiregulin , heregulin alpha , and amphiregulin , all of which are erbB ligands . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
AREG ( Amphiregulin ) , BTC ( beta cellulin ) , EGF , EPGN ( Epigen ) , EREG ( Epiregulin ) , HBEGF , NRG 1 , NRG 2 , NRG 3 , NRG 4 and TGFA ( TGFalpha ) constitute EGF family ligands for ERBB family receptors . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Moreover , using vessels from EGFR ligand deficient mice , we show that transforming growth factor ( TGF ) alpha , but neither heparin binding EGF like growth factor nor epiregulin , transduces NF kappaB activation by high pressure . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
IK cell possessed Estrogen receptor ( ER ) , Progesterone receptor ( PR ) , Epidermal growth factor ( EGF ) and its receptor ( EGFR ) . ^^^ HEC cell had PR , EGF , and EGFR , however HEC cell did not keep ER . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Nearly all studies indicate a negative relationship between EGF R and steroid receptor status ( 28 of 31 studies for ER , 12 / 19 for PR ) showing that EGF R positivity is twice as high in ER or PR negative tumors compared to ER or PR positive tumors ( approximately 50 60 % vs . 30 % ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR was correlated ( P = 0 . 0009 ) with growth fraction assessed by Ki 67 , and negatively correlated with oestrogen receptor ( ER , P = 0 . 0001 ) and progesterone receptor ( PR , P = 0 . 0001 ) status . ^^^ There were , however , several tumours in which EGFR mRNA could be detected in the absence of EGFR protein and there were differences between the ER and PR status of those tumours in which translation of EGFR mRNA was not seen . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
No correlations were observed between the levels of EGF R and the levels of either oestrogen receptors ( ER ) or PgR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Of 221 patients with breast cancer of known epidermal growth factor receptor ( EGFR ) and oestrogen receptor ( ER ) status , 99 had developed recurrences during the period of follow up ( range 3 60 months , median 24 months ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In this investigation , 83 human mammary carcinomas were examined for the expression of oestrogen receptor ( ER ) , epidermal growth factor receptor ( EGF R ) , epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) , c erbB 2 , histological grade , mitotic index and nodal status , all of which are reportedly prognostically significant factors ( Bloom and Richardson 1957 ; Baak et al . 1985 ; Wright et al . 1989 ) . ^^^ ER expression was biochemically recognized in 43 . 4 % of mammary carcinomas , and EGF R , EGF , TGF alpha and c erbB 2 were histochemically recognized in 25 . 3 , 14 . 5 , 27 . 7 and 18 . 0 % of mammary carcinomas examined respectively , using conventional sections of buffered formalin fixed , paraffin embedded tissue and monoclonal or polyclonal antibodies . ^^^ There were significant relationships between negative ER and positive EGF R or TGF alpha ; positive EGF R and TGF alpha ; positive EGF R and c erbB 2 ; and positive c erbB 2 and TGF alpha . ^^^ Therefore , the present investigation indicates that the negative ER , single expression of c erbB 2 and co expression of EGF R and TGF alpha are important markers which contribute indirectly to prognosis , which reconfirms previous findings on the former two while adding the new finding that immunohistochemical demonstration of expression of EGF R and TGF alpha may provide useful information for selecting the appropriate treatment . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In breast cancer we studied the distribution of immunohistochemical positivity for EGFR , c erbB 2 , c myc , ras p 21 and Ki 67 , which was related to age , nodal status , ER and PgR receptor status , LI , DI and histopathological grading . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In both MCF 7 and T 47D cells the decrease in ER mRNA was mirrored by an increase in the level of epidermal growth factor receptor ( EGF R ) mRNA . ^^^ A marked inverse relationship exists between ER and EGF R in human breast cancer biopsies and cell lines , and the reciprocal modulation of these genes by butyrate suggests that the expression of ER and EGF R may be co regulated . ^^^ In the ER positive EGF R negative line , MDA MB 134 6 , butyrate exposure decreased ER mRNA levels , implying that the regulation of ER mRNA by butyrate is independent of EGF R expression . ^^^ However , butyrate decreased EGF R mRNA in two ER negative lines , MDA MB 231 and HBL 100 . ^^^ As this effect differed from that in ER positive lines , the regulation of EGF R may depend on the expression of ER . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We have studied 21 cases of male breast carcinoma immunohistochemically for oestrogen receptor ( ER ) and epidermal growth factor receptor ( EGFR ) expression employing the antibodies ER ICA and 12E on formalin fixed , paraffin embedded material . ^^^ In our series , 86 per cent of male breast cancers were ER positive and 76 per cent were EGFR positive . ^^^ Male breast carcinomas do not exhibit the inverse correlation between ER and EGFR expression that characterizes female breast carcinomas . ^^^ Owing to the limitations of a small series , we were unable to comment on the relationship between ER and EGFR expression and patient survival . ^^^ In addition , high EGFR expression may also contribute to a poor prognosis independent of ER status . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Based on this observation , the presence of occupied sites ( EGF R 2 ) has been assessed in 216 human mammary tumor biopsies simultaneously with the direct measurement of non occupied EGF receptor sites ( EGF R 1 ) and the results compared to estrogen and progesterone receptor status ( ER , PGR ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Premenopausal and postmenopausal EGFR positive tumours ( > or = 10 fmol / mg membrane protein ) could be regarded as homogenous with respect to the concentration of ER and PR whose mean values were low and without being significantly different . ^^^ EGFR negative tumours were heterogeneous with respect to the ER and PR concentrations . ^^^ Postmenopausal EGFR negative ( < 10 fmol / mg membrane protein ) tumours had evidently higher mean values of ER and PR than premenopausal EGFR negative tumours , but these differences were statistically significant for oestrogen receptors only . ^^^ The levels of ER and PR of premenopausal EGFR negative tumours were approximated to the corresponding levels of EGFR positive tumours . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Estrogen ( ER ) , progesterone ( PR ) , and EGF receptors ( EGF R ) have been detected in both mammary epithelial and stromal cells , and the relative roles of the various cells types in hormone dependent growth regulation are not known . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
ZR 75 9al 8 microM cells , routinely maintained in medium containing 8 microM tamoxifen , were negative for oestrogen receptor ( ER ) and progesterone receptor ( PGR ) and expressed a markedly increased number of EGFR ( 14 , 723 + / 2116 receptors / cell ) . ^^^ Time dependent reversal of the tamoxifen resistant phenotype was accompanied by a return to ER and PGR positivity and a fall in EGFR numbers to parent cell levels . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Analysis of epidermal growth factor receptor ( EGFr ) and estrogen receptor ( ER ) was performed on tumor samples from 231 patients with operable breast cancer followed for up to 6 years after surgery . ^^^ Thirty five percent of patients ( 82 ) had tumors greater than 10 fmol / mg of 125I EGF binding ( EGFr+ ) and 47 % ( 109 ) had cystolic ER concentration greater than 5 fmol / mg ( ER+ ) , with a marked inverse relationship between EGFr and ER ( P less than . 00001 ) . ^^^ For patients with histologically negative axillary nodes , EGFr was superior to ER in predicting relapse and survival ( P less than . 01 and P less than . 005 , respectively , compared to P less than . 1 and P less than . 1 , log rank ) . ^^^ In a multivariate ( Cox model ) analysis , only EGFr out of EGFr , ER , size , and grade was predictive for either relapse free or overall survival for patients with node negative disease ( P = . 052 and P = . 026 , respectively ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In this study , correlations between the epidermal growth factor receptor ( EGF R ) , steroid receptors , and other prognostic parameters ( grading , pTNM status , menopausal status ) were analysed in 326 primary breast carcinomas . 19 % of the tumour samples were EGF R positive , 63 % were estrogen receptor ( ER ) and 54 % progesteron receptor ( PR ) positive . ^^^ We found a highly significant inverse correlation between EGF R and steroid receptors . 88 % of the ER positive tumours were EGF R negative ( p less than 5 10 10 ( 5 ) ) , 90 % of the PR positive tumours were EGF R negative ( p less than 5 10 10 ( 5 ) ) and 91 % of the ER plus PR positive tumours were ERF R negative ( p less than 1 10 10 ( 6 ) ) . ^^^ A combination of negative ER and positive EGF R was found more often in the population of G 3 tumours . ^^^ The ER correlated negatively ( p less than 1 . 3 10 10 ( 5 ) and the EGF R positively ( p less than 0 . 042 ) with menopausal status . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR expression correlated with the number of involved lymph nodes , histological grade , estrogen and progesterone receptor ( ER , PR ) levels and the Melbourne Prognostic Index . ^^^ C erbB 2 and ras expression both correlated with ER levels and EGFR , but not with the Prognostic Index . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
There was no significant correlation between expression of c erbB 2 oncoprotein and conventional prognostic factors , estrogen receptor ( ER ) , axillary lymph node metastasis and epidermal growth factor receptor ( EGFR ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor receptors ( EGFr ) and oestrogen receptors ( ER ) measured by ligand binding , c erbB 2 expression assessed by immunochemistry and lymph node status were compared with p 53 staining . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Expression of epidermal growth factor receptor ( EGFr ) has been shown previously to be inversely related to oestrogen receptor ( ER ) in patients with operable breast cancer and to be associated with a poorer prognosis . ^^^ Analysis of EGFr and ER was performed on tumour samples from 231 patients with operable breast cancer followed for up to 6 years after surgery . ^^^ Thirty five percent of patients ( 82 ) had tumours with greater than 10 fmol mg 1 I 125 EGF binding ( EGFr+ ) and 47 % ( 109 ) and cystolic ER concentrations greater than 5 fmol mg 1 ( ER+ ) , with a marked inverse relationship between EGFr and ER ( P less than 0 . 00001 ) . ^^^ For patients with histologically negative axillary nodes EGFr was superior to ER in predicting relapse and survival ( P less than 0 . 01 and P less than 0 . 005 respectively compared to P less than 0 . 1 and P less than 0 . 1 , log rank ) . ^^^ In a multivariate ( Cox model ) analysis only EGFr , out of EGFr , ER , size and grade , was predictive for either relapse free or overall survival for patients with node negative disease ( P = 0 . 05 and P = 0 . 026 respectively ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR positivity was found in 46 % ( 173 of 380 ) of ER positive and in 85 % ( 129 of 151 ) of ER negative breast tumor biopsy samples ( P less than 0 . 0001 ) , as well as in 49 % ( 168 of 344 ) of PgR positive and in 72 % ( 134 of 186 ) of PgR negative breast tumor biopsy samples ( P less than 0 . 0001 ) . ^^^ Mean EGFR levels in ER positive breast tumor biopsy samples were lower than they were in ER negative ones , 40 + / 31 ( SD ) against 72 + / 55 fmol / mg of membrane protein ( P less than 0 . 0001 ) . ^^^ A multivariate analysis showed only ER to be independently associated with EGFR . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Interferons ( IFNs ) may modulate oestrogen ( ER ) , progesterone ( PR ) and epidermal growth factor ( EGFR ) receptor expression in vitro . ^^^ ER , PR and EGFR levels in tumour specimens taken from 13 patients with endometrial adenocarcinomas before and after 5 days ' intramuscular treatment with 5 10 10 ( 6 ) U per recombinant human leucocyte interferon alpha 2b ( rh IFN alpha 2b ) . ^^^ After treatment , ER ( P less than 0 . 01 ) and PR ( P less than 0 . 05 ) levels were significantly increased with a simultaneous reduction of EGFR content ( P less than 0 . 05 ) . ^^^ Since the expression of ER and PR characterises more differentiated hormono sensitive tumours , while EGFR are preferentially expressed in less differentiated tumours , the increase of steroid hormone receptor levels with the reduction of EGFR expression suggests that rh IFN alpha 2b may induce endometrial cancer cell differentiation . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The significance of epidermal growth factor receptor ( EGFR ) status as a prognostic indicator was investigated by a competitive binding assay in 135 primary breast cancer patients . 55 patients ( 41 % ) were EGFR positive and EGFR status was negatively correlated with oestrogen receptor ( ER ) status ( P less than 0 . 01 ) . 5 year postoperative follow up showed that relapse free survival for EGFR positive patients was significantly worse than that for EGFR negative patients ( P less than 0 . 05 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The presence of estrogen , progesterone and epidermal growth factor receptors ( ER , PR , EGFr ) was evaluated using immunohistochemistry before and after therapy with Danazol or a gonadotropin releasing hormone analogue ( GnRHa ) , Buserelin . ^^^ EGFr , ER and PR were present in 100 % of endometrial specimens , and in 71 % , 29 % and 49 % of endometriotic implants , respectively . ^^^ About 21 % of endometriosis were EGFr positive and ER negative , suggesting a potential role of epidermal growth factor in growth and maintenance of endometrial ectopia . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Immunohistochemical assays have been employed to study the expression of ER , PgR , EGFR and Ki 67 immunostaining in normal breast tissue ( n = 76 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The presence of epidermal growth factor , estrogen , and progesterone receptors ( EGFR , ER , and PR ) was investigated by a competitive binding assay in 43 colorectal adenocarcinomas and 32 normal colorectal mucosa specimens . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Expression of prognostic factors ( EGFR , ER ) by immunohistochemical staining method in male breast cancer . ^^^ The expression rate of EGFR ( epidermal growth factor receptor ) , ER ( estrogen receptor ) were 8 . 3 % , 70 . 0 % respectively . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Immunoelectron microscopy revealed that the c neu oncogene product , which is closely related to an epidermal growth factor receptor , was positively stained in the microvilli of tumor cells ( ERpP ) with high growth potential and high metastatic ability , whereas the tumor cells ( ER ) with low growth potential and weak metastatic ability were not stained . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The authors investigated the presence of epidermal growth factor ( EGF R ) , estrogen ( ER ) , progesterone ( PR ) , and androgen ( AR ) receptors in 42 laryngeal squamous cell carcinomas and 20 normal laryngeal mucosa specimens . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In order to evaluate a possible relationship between the histological findings and stroma derived growth regulators , 19 endometrial samples obtained from women undergoing both percutaneous ( n = 11 ) and oral ( n = 8 ) steroid replacement therapy were processed for histological and immunocytochemical evaluation of estrogen receptor ( Er ) , progesterone receptor ( Pr ) , and epidermal growth factor receptor ( EGFr ) . ^^^ Proliferative and hyperplastic endometria showed immunoreactivity against Er , Pr , and EGFr . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Estrogen receptors ( ER ) and epidermal growth factor receptors ( EGFR ) in the tumor cells of breast cancer tissues ( primary tumors ) and metastatic lymph nodes ( involved nodes ) were analyzed by immunocytochemical study in 19 patients ; 12 were ER positive , and seven were ER negative . ^^^ The percentage of EGFR positive cells and the EGFR staining index were higher in the ER negative primary tumors than ER positive primary tumors . ^^^ In ER positive cases , both the number of ER positive cells and ER content per cell was less in the involved nodes than in the primary tumors , whereas the number of EGFR positive cells and EGFR content per cell was greater in affected nodes . ^^^ On the other hand , in the ER negative cases the number of EGFR positive cells and EGFR content per cell remain almost unchanged between the primary tumors and involved nodes . ^^^ The authors supposed a probability , in this study , that estrogen may exert inhibitory action on EGFR production through binding to ER . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
A significant inverse relationship was observed between EGF R and both estrogen ( ER ) and progesterone receptors ( PR ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
This was especially evident where the ER negative tumours were EGF R positive and showed high rates of cell proliferation . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The EGFr was significantly more frequent ( chi 2 = 5 . 9 ; P less than 0 . 025 ) and its concentration was significantly higher ( P less than 0 . 001 ) among ER tumors than in ER+ tumors . ^^^ Increases in the proportion of EGFr+ in ER tumors are parallel to Scarff Bloom scores ( chi 2 = 6 . 1 ; P less than 0 . 05 ) and there is a significant trend ( Spearman rs = 0 . 25 ; P less than 0 . 05 ) towards increased EGFr concentrations with histologic dedifferentiation . ^^^ These findings support the hypothesis that EGFr is a bad prognosis factor and suggest that EGFr expression and concentration in ER+ tumors might be considered an estrogenic action mediated through the binding of ER to their nuclear acceptors . . ^^^ Epidermal growth factor receptor in human breast cancer : correlation with cytosolic and nuclear ER receptors and with biological and histological tumor characteristics . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Growth fractions detected by a monoclonal antibody , Ki 67 , were examined in 40 human breast cancer tissues and the results compared with the immunocytochemical reactivities of epidermal growth factor receptor ( EGFR ) and estrogen receptor ( ER ) . ^^^ This data indicated the difference in growth fractions with relation to the EGFR and ER status of breast cancer . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
As factors regulating growth and differentiation of cervical squamous epithelium , immunohistochemical expression of estrogen receptor ( ER ) , progesterone receptor ( PR ) , epidermal growth factor receptor ( EGFR ) , c erbB 2 protein , adult T cell leukemia derived factor ( ADF ) , and HPV DNA was examined . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The expression of epidermal growth factor receptor ( EGFR ) was studied in the normal and inflamed cervix uteri , and the results were compared with estrogen receptor ( ER ) expression . ^^^ The study was performed on fresh frozen specimens from 18 cases by means of immunohistochemistry , with application of EGFR 1 antibody and an ER ICA kit ( Abbott , Wiesbaden , F . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Furthermore , it has been suggested that EGF R levels are higher in estrogen receptor negative ( ER ) than in ER+ human breast tumors and that EGF R status may be a prognostic indicator in breast cancer . ^^^ The present study was undertaken to establish whether there is a quantitative relationship between EGF R and ER mRNA concentrations in a series of 10 well characterized human breast cancer cell lines . ^^^ When these lines were divided into an ER+ and an ER group based on their ability to bind estradiol , ER cell lines were shown to express significantly higher concentrations of EGF R mRNA than did ER+ cell lines ( p less than 0 . 005 ) . ^^^ Furthermore , linear regression analysis revealed a significant inverse relationship between ER and EGF R mRNA concentrations both within the group of 10 human breast cancer cell lines as a whole ( r = 0 . 66 ) and within the 6 functionally ER + lines ( r = 0 . 77 ) . ^^^ This demonstration of a significant ( p less than 0 . 005 ) inverse relationship between the concentrations of ER and EGF R mRNAs in ER + cell lines raises the possibility of reciprocal regulation of the expression of these genes in human breast cancer . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
ER , PR and AR mRNAs were negatively associated with GR levels ( p less than or equal to 0 . 012 ) , while ER and AR mRNA levels were negatively correlated with mRNA for the epidermal growth factor receptor . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Using serial frozen sections , monoclonal antibodies and an indirect immunoperoxidase method , 13 fibroadenomas ( FA ) and 3 cystosarcomas phyllodes ( CSP ) were analyzed for the expression of Egp 34 , HEA 319 antigen , leucocyte differentiation antigens CD 10 , CD 30 , CD 57 , CD 72 , CDw 75 , and CD 77 , epidermal growth factor receptor ( EGFR ) , estrogen ( ER ) and progesterone receptor ( PR ) , and transferrin receptor ( CD 71 ) . ^^^ However , expression of ER and EGFR was inverse . ^^^ In contrast , stromal cells of FA were able to co express EGFR and CD 71 in the absence of PR and ER . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
With threshold values of 5 fmol / mg and 10 fmol / mg for EGF R and steroid receptors respectively , we noted an inverse relationship between the expression of EGF R , and ER and PR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Estrogen ( ER ) and progesterone ( PgR ) receptors were detected by an immunocytochemical assay and an equal distribution of EGFr was found regarding steroid hormonal receptors expression . ^^^ Our results suggest the presence of a subclass of breast tumors , characterized by the absence of ER and / or PgR and the presence of EGFr , whose growth appears to be mediated by autocrine growth factors rather than by steroid hormones . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Between EGFR and estrogen receptor ( ER ) status , a clear inverse relationship was observed ( P less than 0 . 01 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In ER cell lines , TPA induced growth inhibition and the minor changes in EGF R concentration were unrelated . ^^^ These data demonstrate differential relationships between TPA induced changes in growth and EGF R binding in ER+ and ER breast cancer cells , thus supporting the view that growth regulatory pathways are markedly different in these two subtypes of human breast cancer . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Analysis of EGFr and ER was performed on tumour samples from 231 patients with operable breast cancer followed for up to 6 yr after surgery . ^^^ Thirty five percent of patients ( 82 ) had tumours greater than 10 fmol / mg 125I EGF binding ( EGFr+ ) and 47 % ( 109 ) had cystolic ER concentration greater than 5 fmol / mg ( ER+ ) , with a marked inverse relationship between EGFr and ER ( P less than 0 . 00001 ) . ^^^ For patients with histologically negative axillary nodes EGFr was superior to ER in predicting relapse and survival ( P less than 0 . 01 and P less than 0 . 005 , respectively , compared to P less than 0 . 1 and P less than 0 . 1 , logrank ) . ^^^ In a multivariate ( Cox model ) analysis only EGFr , out of EGFr , ER , size and grade , was predictive for either relapse free or overall survival for patients with node negative disease ( P = 0 . 052 and P = 0 . 026 , respectively ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The presence of EGFR mRNA was inverted related to oestrogen receptor status and coexpression of TGFa and EGFR was observed in 28 % of carcinomas , and significantly more commonly in ER negative tumours ( P = 0 . 01 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
MCA expression was also compared with other biological parameters such as the presence of estrogen receptors ( ER ) , progesterone receptors ( PgR ) , epithelial growth factor receptors ( EGF R ) , and oncoprotein p 53 which is encoded by the oncogene N myc . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The presence of EGFR mRNA was inversely related to oestrogen receptor ( ER ) status ( P = 0 . 0001 ) . ^^^ Coexpression of TGF alpha and EGFR was observed in 28 % of the carcinomas , and significantly more commonly in ER negative tumours ( P = 0 . 01 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The simultaneous determination of EGF R and that of estradiol ( ER ) , progesterone ( PR ) and androgen receptors ( AR ) was performed using the same buffer to homogenate the tissues and to obtain cellular membranes . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
This study was aimed at elucidating the immunohistochemical localization of oncogene products which are related to cell growth , c erbB 2 product , epidermal growth factor receptor ( EGFR ) , c myc protein and estrogen receptor ( ER ) , in benign and malignant lesions of the breast . ^^^ C erbB 2 product and EGFR were localized on the cell membrane whereas c myc protein and ER were observed in the nuclei . ^^^ However immunoreactivities of EGFR and ER were more frequently seen in the fibroadenomas ( p less than 0 . 05 ) . ^^^ In breast cancers , the incidence of immunoreactivity for c erbB 2 was higher in the cases with lymph node metastasis than cases without nodal metastasis ( p less than 0 . 05 ) and there was reciprocal correlation between the expressions of EGFR and ER ( p less than 0 . 05 ) . ^^^ Regarding the size of the primary tumour , there was no statistically significant correlation with the expressions of c erbB 2 , EGFR , c myc or ER . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The EGFr was significantly more frequent ( X 2 = 5 . 9 ; P less than 0 . 025 ) and its concentration was significantly higher ( P less than 0 . 001 ) among ER tumors than in ER+ tumors . ^^^ Increases in the proportion of EGFr+ in ER tumors are parallel to Scarff Bloom scores ( X 2 = 6 . 1 ; P less than 0 . 05 ) and there is a significant trend towards increased EGFr concentrations with histologic dedifferentiation . ^^^ These findings support the hypothesis that considers EGFr as a bad prognosis factor and suggest that EGFr expression and concentration in ER+ tumors might be considered an estrogenic action mediated through the binding of ER to their nuclear acceptors . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Eight of the 17 EGFR positive tumors ( 47 . 1 % ) were positive for estrogen receptor ( ER ) , whereas 24 of the 28 EGFR negative tumors ( 85 . 7 % ) were ER positive . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Southern blot analysis and RNA dot blot analysis demonstrated that the ER variants had lost the amplified EGFR gene . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor receptors ( EGFR ) and oestrogen receptors ( ER ) were analysed in 221 patients with primary operable breast cancer by means of radioligand assays . ^^^ Only 1 of 28 EGFR positive , ER negative tumours achieved an objective response . ^^^ Including patients whose disease remained stable for more than 6 months with the responders , however , EGFR status was a better predictor of response to tamoxifen ; 15 of 37 EGFR negative patients and 5 of 35 EGFR positive patients responded ( p less than 0 . 01 ) , compared with 13 of 32 ER positive and 7 of 40 ER negative patients ( not significant ) . ^^^ EGFR expression is a highly significant marker of poor prognosis in patients with breast cancer ; it appears to be as good a predictor as ER for objective response and better for overall response to endocrine therapy on relapse . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In general , effects on ER , epidermal growth factor receptor , gamma glutamyl transpeptidase and glucose 6 phosphatase were greater in DEN / EE2 treated animals than in rats receiving only EE 2 . ^^^ Modification of receptor pathways associated with hepatocyte growth control , ER and epidermal growth factor receptor , may be contributing factors in the clonal expansion of preneoplastic cells during EE 2 promotion of hepatocarcinogenesis . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
There was a highly significant inverse relationship between oestrogen receptor ( ER ) and EGFR ( 15 EGFR positive [ EGFR+ ] ER+ and 92 EGFR negative [ EGFR ] ER+ : 54 EGFR ER and 60 EGFR+ ER ) . ^^^ When ER tumours were substratified by EGFR status , the EGFR ER tumours had a prognosis almost as good as the ER+ tumours . ^^^ Epidermal growth factor receptors ( EGFR ) and oestrogen receptors ( ER ) were analysed in 221 patients with primary operable breast cancer by means of radioligand assays . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
A clear inverse relationship between EGFR content and ER and PR status has been observed in breast tumors . ^^^ We also show here that there is a similar inverse relationship between EGFR content and ER levels in endometrial tumors . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In contrast , a significant inverse relationship ( p less than 0 . 05 ) was found between EGFR and estrogen receptor ( ER ) patterns . ^^^ Recognition that EGFR is negatively related to ER supports the present evidence that the control of malignant cell growth and differentiation depends on complex regulatory mechanisms in which several extracellular messenger molecules , including hormones and peptide growth factors together with their specific cellular receptors , are involved and inextricably interwoven . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Expression of epidermal growth factor receptor ( EGFR ) and estrogen receptor ( ER ) was examined by an immunocytochemical assay ( ICA ) using serial cross sections of human breast cancer tissues . ^^^ A significant inverse relationship of the proportion of stained cells between EGFR and ER was demonstrated . ^^^ In more than one half of the tumors that were positive for both EGFR and ER , these 2 receptors were inversely stained in relation to the distribution . ^^^ In ER negative cells , EGFR expression was more marked than in ER positive cells . ^^^ Biochemical data confirmed the immunocytochemical results , pointing to an inverse relationship between EGFR and ER content . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The tumor cell content of the receptor for epidermal growth factor ( EGF R ) and estrogen receptor ( ER ) was analyzed in 36 primary breast carcinomas and their metastatic deposits . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR was significantly correlated to ER and histological grade . ^^^ EGFR positive tumors and high levels of EGFR were mainly found in the ER negative group of tumors ( p = 0 . 008 ) and in histological grade 3 ( p = 0 . 007 ) . ^^^ The EGFR phenotype appeared as a patients with different early outcome , with potential therapeutic implication especially in the group of ER negative patients . ^^^ These results emphasize the need for a standardized assay methodology and for further clinical studies , particularly in protocols where adjuvant hormonal therapy is prescribed on the basis of steroid hormone receptor status , in order to assess the respective prognostic worth of EGFR and ER ( or PR ) . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Previous studies have demonstrated an inverse relationship between estrogen receptor ( ER ) and epidermal growth factor receptor ( EGF R ) gene expression in human breast cancer cells . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Fifty one samples of non malignant tissue from four mastectomies were analysed to assess oestrogen receptor ( ER ) and epidermal growth factor receptor ( EGFR ) status across the cancerous breast . ^^^ No significant relationship was found between the presence of EGFR and ER . ^^^ EGFR and ER expression was not affected by histological sub group . ^^^ In contrast , analysis of 44 primary cancers showed , in agreement with the literature , a significant inverse relationship between the presence of ER and EGFR ( Fisher ' s exact test P less than 0 . 002 ) . ^^^ The difference between malignant and non malignant tissue appeared to result from the prevalence of co expression of EGFR and ER in the non malignant specimens . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
It was found that estrogen receptor ( ER ) was inverse related to TrfR , Ki 67 and EGFR whereas progesterone receptor ( PgR ) was inverse correlated to Ki 67 solely . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Synchronous expression of ER and epidermal growth factor receptor was found in 8 of the 26 scirrhous carcinomas ( 30 . 8 % ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In 135 primary breast cancers , there was a significant inverse relation between epidermal growth factor receptor ( EGFR ) and oestrogen receptor ( ER ) status , and a significant association with tumour size and poor differentiation . ^^^ Of the 71 ER patients 28 were EGFR+ and 43 were EGFR . ^^^ Thus EGFR status divides the ER population into good and poor prognosis subgroups . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGF R correlated negatively to the ER and PgR ( Kendall correlation , P less than 0 . 001 ) , whereas the IGF R correlated positively to ER and PgR ( analysis of variance , P less than 0 . 001 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The activities of total PA and of each type of PA were compared to the estrogen receptor ( ER ) and epidermal growth factor receptor ( EGFR ) status of the tumors and to their histological grade . ^^^ Tissue type PA levels , however , were significantly lower in ER negative compared with ER positive tumors and in EGFR positive compared with EGFR negative tumors ( P less than 0 . 01 and less than 0 . 05 , respectively ) . ^^^ The ER negative tumors were further stratified into EGFR positive and negative subgroups . ^^^ Only the ER negative tumors possessing EGFR had significantly lower tPA levels than the ER positive tumors ( P less than 0 . 01 ) . ^^^ Low tPA levels in breast cancers were , therefore , associated with ER negativity combined with EGFR positivity and may be an indication of poorer differentiation and prognosis . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Fifty five primary breast tumors and 7 lymph node metastases were simultaneously assayed for the presence of EGF receptors ( EGFR ) , estrogen receptors ( ER ) , and progesterone receptors ( PR ) . ^^^ EGFR were more frequently present in ER and PR negative than in ER and PR positive tumors . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Assays for EGFr ( two point radioreceptor assay ) and oestrogen receptors ( ER ) ( dextran coated charcoal method and an immunohistochemical method ) were performed on 20 / 22 patients . ^^^ In this group early relapse was predicted by EGFr status , but not by ER status ( median disease free survival for EGFr+ patients 15 months , and for EGFr patients 40 months , P less than 0 . 01 , logrank test ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Estrogen receptor ( ER ) , epidermal growth factor ( EGF ) and epidermal growth factor receptor ( EGFR ) were examined by immunocytochemical and biochemical assays in 86 breast carcinomas . ^^^ A significant inverse relationship was obtained between ER and EGFR values ( p less than 0 . 05 ) . 4 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
PAb421+ cases were significantly more frequently epidermal growth factor receptor ( EGF R ) positive ( 67 . 7 % ; p less than 0 . 001 ) and estrogen receptor ( ER ) negative ( 73 . 3 % ; p less than 0 . 001 ) ; they displayed surface histocompatibility class 1 ( 80 . 6 % ; p less than 0 . 01 ) and 11 ( 74 . 2 % ; p less than 0 . 05 ) antigens . ^^^ Analysis of PAb1801+ / Pab421 cases for HLA antigens , EGF R and ER showed a phenotype similar to that of the p 53 ve / ER+ carcinomas , except for the high Ki 67 score . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The coordinate expression of the nuclear p 53 protein , cytoplasmic intermediate filament vimentin ( VIM ) and membrane epidermal growth factor receptor ( EGF R ) was significantly associated with oestrogen receptor immunocytochemical nuclear stain ( ER ICA ) negative breast carcinomas . ^^^ Twenty three ( 51 . 1 % ) , 26 ( 57 . 8 % ) and 27 ( 60 % ) of 45 ER ICA ve cancers were respectively p 53 +ve , VIM +ve and EGF R +ve ; whereas of 151 ER ICA +ve tumours 8 ( 5 . 3 % ) were p 53 +ve ( P less than 0 . 0001 ) , 23 ( 15 . 2 % ) VIM +ve ( P less than 0 . 001 ) and 40 ( 26 . 5 % ) EGF R +ve P less than 0 . 001 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
However , reports on the presence of EGF r in human breast cancer biopsies are still controversial , especially concerning the relationship between EGF r and other biological variables whose prognostic relevance is well known , such as the estrogen ( ER ) and progesterone ( PgR ) receptors . ^^^ In the present study , the EGF r content was estimated in a series of 136 unselected breast cancer primaries along with cytoplasmic ( ERc ) and nuclear ( ERn ) ER and cytoplasmic PgR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
An inverse correlation between EGFR and estrogen receptor ( ER ) was observed in 54 % of the tumors . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Our results confirm previous reports of an inverse relation between the cellular content of EGF R and ER . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In a series of 60 breast cancers , we have studied relationships between ER , ER D 5 oestrogen receptor related protein , P 24 oestrogen regulated protein , and EGFR using an immunohistochemical technique employing monoclonal antibodies in each case . ^^^ In addition , radioligand binding assays for ER and EGFR were carried out and tumour histological grade was determined . ^^^ Seventy one per cent and forty three per cent of tumours stained for ER D 5 and P 24 , respectively , but there was no relationship between staining for these and ER or EGFR status . ^^^ There was a significant correlation between staining for ER and EGFR , and the respective biochemical assays . ^^^ Relating ER to EGFR , very few ER positive cases expressed EGFR , but this relationship fell short of significance . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR is activated in and translocated from the endoplasmic reticulum ( ER ) slowly ( t1 / 2 approximately equal to 75 min ) . ^^^ Since translocation rate equals the rate for acquisition of endo H resistance , transit from the ER is rate limiting for EGFR maturation . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
These results were compared with growth fractions ( Ki 67 immunoreactivity and Transferrin receptor ( TrfR ) expression ) , histologic tumor type , tumor grading , axillary lymph node status and estrogen receptor ( ER ) status . 60 . 2 % were EGFR positive . ^^^ ER status revealed an inverse correlation to EGFR expression ( Kendall ' s tau : 0 . 22804 , p = 0 . 012 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
There was a strong inverse relationship between EGFr greater than 10 fmol / mg membrane protein ( 2 point assay ) and ER ( dextran coated charcoal method ) , Chi squared 2 10 2 contingency table test = 34 . 027 , p less than 0 . 0001 . ^^^ Follow up data from 135 patients revealed a strong inverse relationship between EGFr greater than 10 fmol / mg membrane protein and oestrogen receptor ( ER ) status and a positive correlation with early recurrence and death . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Androgen receptor ( AR ) , oestrogen receptor ( ER ) , progesterone receptor ( PR ) and epidermal growth factor receptor ( EGFR ) concentrations were assayed by saturation binding with a competitive inhibitor . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Time resolved immunofluorometric procedures were used to quantify both p 53 protein and PSA in 200 breast tumour extracts , which were also assayed for oestrogen ( ER ) and progesterone receptors ( PGR ) , epidermal growth factor receptors ( EGFR ) , cathepsin D and HER 2 / neu , and characterised for S phase fraction and DNA ploidy . ^^^ Wilcoxon rank sum analysis revealed that levels of ER ( P = 0 . 0001 ) , PGR ( P = 0 . 0001 ) , S phase fraction ( P = 0 . 0001 ) and EGFR ( P = 0 . 0014 ) differed significantly between the two groups categorised as p 53 negative or p 53 positive . ^^^ Contingency tables indicated significant negative associations between the status of p 53 and that of ER ( P = 0 . 003 ) and PGR ( P = 0 . 001 ) and between PSA and S phase fraction ( P = 0 . 012 ) , and positive associations between p 53 and EGFR ( P = 0 . 017 ) , HER 2 / neu ( P = 0 . 008 ) , S phase fraction ( P = 0 . 001 ) and aneuploidy ( P = 0 . 007 ) , and between PSA and both ER ( P = 0 . 061 ) and PGR ( P = 0 . 010 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Quantification of oestrogen receptor ( ER ) , epidermal growth factor receptor ( EGFR ) , c erbB 2 receptor levels and ploidy were examined on the total and cytokeratin positive cell populations . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Ploidy , S phase fraction , Ki 67 staining , estrogen receptor ( ER ) , progesterone receptor ( PR ) , and the expression of HER 2 / neu and epidermal growth factor receptor ( EGFR ) were evaluated in these tumors . ^^^ RESULTS : Comedo invasive cancers were comparable to pure invasive cancers for ploidy , S phase fraction , Ki 67 staining , and ER , PR , and EGFR expression . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We therefore measured microvessel density in a series of well characterised node negative breast carcinomas to evaluate angiogenesis as a prognostic marker and assess its relationship to epidermal growth factor receptor ( EGFR ) and estrogen receptor ( ER ) , which have previously been reported to be of value . 109 patients with a mean age of 55 years and a median follow up of 25 months were examined . ^^^ No significant correlation was observed with patient age , tumor size , grade , ER , or EGFR expression . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
A new dual immunocytochemical assay for oestrogen receptor ( ER ) and epidermal growth factor receptor ( EGFR ) has been developed . ^^^ MCF 7 and A 431 cells and a mixture of the two types of cell were assessed immunocytochemically for ER and EGFR . ^^^ Dual immunocytochemical assays using anti ER followed by anti EGFR monoclonal antibodies on single histological sections showed similar reactivity to the single assays . ^^^ Three staining patterns were seen in the mixture : ER+ / EGFR ( MCF 7 cells ) , ER / EGFR ( MCF 7 cells ) and ER / EGFR+ ( A 431 cells ) . ^^^ This is the first description of a dual immunocytochemical assay system for ER and EGFR on single 5 microns frozen section samples . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
ER negativity was significantly correlated with the expression of p 53 , epidermal growth factor receptor ( EGFR ) and c erbB 2 ( p < 0 . 05 each ) . ^^^ Thus , it was concluded that ER positive breast carcinomas , relatively small in size , preferentially expressed HSP 27 , HSP 70 and pS 2 and that ER negative tumors , relatively large in size , were predisposed to express p 53 , EGFR and c erbB 2 . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The expression of epidermal growth factor receptor ( EGFR ) , oestrogen receptor ( ER ) and progesterone receptor ( PR ) was assayed by a radioreceptor method in 117 primary ovarian cancers . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Changes in the concentrations and affinities of receptors for oestrogen ( ER ) , progesterone ( PR ) and epidermal growth factor ( EGF R ) were studied in mammary glands of healthy bitches with regard to age , the location in the mammary chain and the stage of the oestrous cycle . ^^^ ER , PR and EGF R were detected by biochemical assays using increasing concentrations of tritiated ( steroids ) or iodinated ( EGF ) ligands . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Oestrogen receptor ( ER ) and epidermal growth factor receptor ( EGFR ) gene methylation was evaluated in neoplastic and perineoplastic breast tissues from 20 patients . ^^^ In both tissues , ER gene methylation was inversely correlated with protein levels , while EGFR gene methylation was not . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
As shown previously , oestrogen receptor ( ER ) negativity , high Ki 67 labelling index and EGFR overexpression were associated with a shorter time to progression and overall survival . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The levels of estrogen receptor ( ER ) , epidermal growth factor ( EGF ) receptor ( EGF R ) and GH receptor ( GHR ) in the mammary glands of the animals were also measured . ^^^ We hypothesize that the reduction in the circulating concentration of GH caused the reduced susceptibility of parous rats to mammary carcinogenesis possibly by decreasing the levels of ER and / or EGF R in the mammary gland . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGF R expression was compared to oestrogen ( ER ) and progesterone ( PgR ) receptor expression , histological grade , tumour size , lymph node metastases and menopause . 52 of 142 tumours were EGF R positive . ^^^ There was no significant correlation between ER or PgR and EGF R expression . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In a multivariate analysis including axillary lymph node status , histological grade , tumour size , ER status , c erbB 2 score , EGF ratio and hormonal treatment , c erbB 2 overexpression was the most powerful parameter ( P = 0 . 001 ) followed by EGF R overexpression ( P = 0 . 02 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We studied the expressions of aberrant epidermal growth factor receptor ( EGFR ) gene or erbB 2 , which is highly homologous to EGFR gene , and erbA or estrogen receptor ( ER ) gene , which is highly homologous to erbA , as a preliminary study , to know which oncogene expressions are associated with the development of endometrial cancers . ^^^ The behavior of aberrant erbB 2 and ER gene co expressions is considered of similar to that of erbA and erbB co expressions in the chicken introduced by the avian erythroblastosis virus , which leads to the development of erythroblastosis in the chick , and seems to be associated with the development of endometrial cancer . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Overexpression of the EGF receptor in breast cancer correlates with poor prognosis and failure on endocrine therapy for both ER / EGFR+ and ER+ / EGFR+ tumors , suggesting a role for EGFR in the progression to hormone independence . ^^^ ER cells implicates regions of the EGFR first intron in up regulation of EGFR , while estrogen regulation studies indicate the involvement of a repressor ( s ) in the maintenance of low levels of EGFR . ^^^ Based on these findings , a multi step model is proposed for the progression of breast cancer from a hormone dependent , ER+ / EGFR phenotype to an aggressive , hormone independent , ER / EGFR+ stage . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Fine needle aspirates ( FNA ) from 106 high risk women and 25 low risk women were evaluated for overexpression of estrogen receptor ( ER ) , epidermal growth factor receptor ( EGFR ) , mutant p 53 , and HER 2 / neu by immunocytochemistry , and for aneuploidy by image analysis . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor receptor expression in breast cancer : association with response to endocrine therapy . 106 previously untreated breast cancer patients have been immunohistochemically analysed for EGF R , ER , Ki 67 , and c erbB 2 product . ^^^ Significant associations were observed between EGF R and ER ( inverse ) and Ki 67 ( direct ) . ^^^ In ER positive breast cancers , EGF R expression had no significant influence on the quality of tumour remissions . ^^^ Further sub classification of the ER / EGF R data by Ki 67 immunostaining showed that in ER+ / EGF R disease , increasing proportions of Ki 67 positive cells were associated with a decline in the numbers of women experiencing good quality tumour remissions . ^^^ The presence of c erbB 2 protein product did not influence endocrine sensitivity in any of the ER / EGF R sub groups . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We investigated the relation of c erbB 2 and int 2 gene amplification , expression , ER and EGFR with clinical characteristics . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The expression of transforming growth factor alpha ( TGF alpha ) , epidermal growth factor receptor ( EGF R ) and oncogenes c erbB 2 , c H ras , c myc , as well as estrogen ( ER ) and progesterone ( PR ) receptors were studied immunohistochemically in the tissue of 21 benign and 58 malignant human breast lesions . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The effects of a short period of in vivo systemic rh IFN alpha / 2b treatment on epidermal growth factor ( EGFR ) , estrogen ( ER ) and progesterone ( PR ) receptor content in 11 primary human cervical tumors was investigated by the radioreceptorial technique . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Parameters correlated with ER functional aspect ( progesterone receptors and PS 2 ) , receptors of epidermal growth factor ( EGFR ) , protease cathepsin D and tumor proliferation ( deduced from thymidine kinase activity ) were analyzed . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The expression of epidermal growth factor receptor ( EGF R ) , c erbB 2 proto oncogene , and estrogen receptor ( ER ) was studied immunohistochemically in a series of 97 human papillomavirus ( HPV ) lesions of the uterine cervix , with special emphasis on their association with the HPV type , grade of intraepithelial neoplasia ( CIN ) , and the natural history of the disease . ^^^ No clear cut associations were established between the EGF R , c erbB 2 , or ER expression and HPV type , nor in CIN or the clinical course of HPV infections . ^^^ This failure for EGF R , c erbB 2 , and ER to be associated with the specific HPV types , grade of CIN , or the clinical course of cervical HPV lesions suggests that the assessment of these factors is of limited value in explaining the development of HPV associated CIN and in predicting the prognosis of this disease . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Association among an autocrine parameter ( EGF R ) and endocrine parameters ( ER and PR ) in locoregional breast cancer . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor ( EGFR ) , oestrogen ( ER ) , and progestin ( PR ) receptor concentrations were determined by radioligand binding assay in non affected mammary tissues ( n = 13 ) and benign ( n = 11 ) and primary / locally recurrent malignant proliferative mammary lesions ( n = 45 ) and metastases ( n = 19 ) in 65 female dogs . ^^^ No relation was found between the expression of EGFR and that of ER or PR in non affected or in tumorous mammary tissues . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Prevalence of aneuploidy , overexpressed ER , and overexpressed EGFR in random breast aspirates of women at high and low risk for breast cancer . ^^^ Biomarkers included DNA ploidy , estrogen receptor ( ER ) , and epidermal growth factor receptor ( EGFR ) . ^^^ The prevalence of DNA aneuploidy was 30 % , overexpression of ER 10 % , and overexpression of EGFR 35 % , in the 206 high risk women whose median 10 year Gail risk ( projected probability ) of developing breast cancer was 4 . 5 % . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
However , among the tumor variants analyzed , ER and EGFR levels appear to be inversely related . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We have used the monoclonal antibody 124 to investigate BCL 2 expression in 212 breast carcinomas , and to correlate it with the oestrogen ( ER ) , progesterone ( PR ) and epidermal growth factor receptor ( EGFR ) status , and with other clinicopathological variables including tumour type , grade , stage , growth fraction ( as evaluated by Ki 67 immunostaining ) , and p 53 accumulation . ^^^ BCL 2 immunoreactivity was strongly correlated with ER and PR expression ( P < 0 . 00001 ) , with the lobular type ( P = 0 . 012 ) and with better differentiated neoplasms ( P = 0 . 00003 ) , whereas it was inversely correlated with EGFR ( P < 0 . 00001 ) , p 53 ( P = 0 . 0004 ) and Ki 67 ( P = 0 . 0002 ) immunoreactivities . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The objective of this work was to determine whether transformation of the human breast epithelial cell line MCF 10F by the chemical carcinogens 7 , 12 dimethylbenz ( a ) anthracene ( DMBA ) or benzo ( a ) pyrene ( BP ) , or c Ha ras oncogene transfection , influence the expression of epidermal growth factor receptor ( EGFR ) , estrogen ( ER ) or progesterone ( PR ) receptors , and the content of cathepsin D ( Cath . ^^^ MCF 10F cells contained 37 fmol / mg of protein of EGFR , ER and PR were undetectable , and Cath . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
High tumour levels of ER , PGR , AR and PS 2 protein predict a relatively good response to endocrine therapy , whereas EGFR positivity , HER2 / neu positivity , aneuploidy , high proliferation indices and possibly high u PA levels indicate a good chance of a poor response to endocrine therapy in metastatic breast cancer . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor receptor ( EGFR ) overexpression correlates with both loss of estrogen receptor ( ER ) and poor prognosis in breast cancer . ^^^ Interestingly , in normal breast EGFR appears to be expressed more frequently than in malignant tissue , and there may be a different relationship between ER and EGFR . ^^^ Epidermal growth factor receptor ( EGFR ) overexpression correlates with both loss of estrogen receptor ( ER ) and poor prognosis in breast cancer . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor receptor ( EGFR ) and estrogen receptor ( ER ) were assayed by ligand binding in tumors from 370 patients with primary breast carcinoma with a median follow up of 18 months . ^^^ Forty seven percent ( 175 / 370 ) and 57 % ( 210 / 370 ) of tumors had > 20 fmol / mg and > 10 fmol / mg of EGFR and ER respectively . ^^^ There was a highly significant inverse relationship between EGFR and ER ( p = 0 . 0032 ) . ^^^ In a multivariate analysis ( Cox proportional hazard model ) of all patients , lymph node status was an independent prognostic indicator for OS and RFS ( p < 0 . 00005 ) and p = 0 . 00005 respectively ) , ER status for RFS ( p = 0 . 0006 ) , and EGFR ( in the node negative model ) for RFS ( p = 0 . 03 ) . ^^^ When all patients were stratified for EGFR and ER , there was a significant difference in RFS and OS such that EGFR positive and ER negative had the worst prognosis ( p = 0 . 0034 and p = 0 . 005 respectively ) . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
This study , therefore , attempts to define and correlate the physiological processes in the rat uterus following estrogen and tamoxifen administration with temporal events manifested by mRNA expression of protooncogenes ( m myc , c ras , c fos , c jun ) , growth factors and / or inhibins ( IGF 1 , IGF 2 , IGF 2 Exon 1 and Exon 2 ) , EGF , TGFB 1 , 2 , 3 ) , growth factor or inhibin receptors ( EGFr , TGFB 2r , TGFB 3r ) , and estrogen induced differentiative proteins including estrogen receptor ( ER ) , progesterone receptor ( PR ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The evaluation of molecular markers in breast carcinomas can be routinely assessed by ( 1 ) histochemistry for ploidy measurement ( Feulgen stain ) and for AgNORs counts , and by ( 2 ) immunocytochemistry ( Ki 67 , cathepsin D , pHER 2 / neu , EGFR , ER , PR , pS 2 , p 53 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
However , expression of EGFR in addition to c erbB 2 protein was more frequently observed with advancing stage of disease and was inversely correlated with the grade of differentiation and with the expression of ER or PR of the tumor . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In the ER negative group , EGFR positive patients had a shorter relapse free time than patients with EGFR negative cancer ( p < 0 . 009 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
METHODS : Cell membrane levels of epidermal growth factor receptor ( EGF R ) , and cytosol ( c ) estrogen ( ER ) and progesterone ( PR ) receptors were determined in 221 specimens of primary breast cancers . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Recent studies have demonstrated a consistent inverse relationship between oestrogen receptor ( ER ) and epidermal growth factor receptor ( EGFR ) levels in female human breast cancer . ^^^ Serial cross section studies have suggested that separate populations of ER+ / EGFR and ER / EGFR+ cancer cells exist in tumours deemed by immunocytochemical assay ( ICA ) to be positive for both . ^^^ We have developed a dual ICA that is able to stain for both ER and EGFR on a single 5 microns frozen section sample of breast tissue . ^^^ Twenty two samples of female human breast cancer tissue that exhibited positivity for ER and EGFR by ER ICA using the H 222 monoclonal antibody and EGFR ICA using the EGFR 1 monoclonal antibody underwent the dual ICA . ^^^ There was a significant correlation in receptor positivity between the single and dual assays for both ER ( rs = 0 . 801 , P < 0 . 001 ) and EGFR ( rs = 0 . 831 , P < 0 . 001 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Receptors for oestrogen ( ER ) , progesterone ( PR ) and epidermal growth factor ( EGF R ) are found in normal mammary tissue ( NMT ) and / or mammary tumours ( MT ) from all species studied , including dogs . ^^^ The aims of this study were to define the possible influences of mammary histology , age , location in the mammary chain and of hormonal status and cycle stage in the expression of ER , PR and EGF R in mammary tissues from healthy dogs and from dogs with MT . ^^^ EGF R and ER were significantly and positively correlated only in malignant tumours . ^^^ There is no apparent difference between affected and healthy dogs in the regulation of ER , PR and EGF R expression in NMT ; on the other hand , some differences between NMT and MT are observed in the regulation of PR ( for example under the influence of MPA ) and in the correlation between EGF R and ER expression . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGF R was measured by both biochemical and two immunohistochemical methods , using two monoclonal antibodies ( MAb ) , in addition to oestrogen receptors ( ER ) and progesterone receptors ( PgR ) . ^^^ ER and PgR were only weakly correlated ( Rs = 0 . 20 ) and they showed no significant association with EGF R status . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
A strong negative relationship was observed , with 26 of 88 ( 30 % ) bcl 2 positive tumours being EGFR positive , compared with 16 of 23 ( 70 % ) bcl 2 negative tumours being EGFR positive ( P = 0 . 001 ) , raising the possibility that bcl 2 is an ER regulated gene . ^^^ Thus , loss of bcl 2 expression in breast cancer is associated with a range of molecular markers of poor prognosis and may define part of an ER negative , EGFR positive phenotype . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The presence of EGFR correlated with positive lymph node status ( P = 0 . 004 ) , poor differentiation ( P = 0 . 001 ) , and negative ER status ( P = 0 . 0001 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Since ST 3 mRNA expression was independent of the EGFR , ER and erbB 2 protein expression , ST 3 may be a new potent prognostic guide for breast carcinomas , which can detect highly malignant subpopulations . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Expressions of epidermal growth factor receptor ( EGFR ) and estrogen receptor ( ER ) in human thyroid tumors were examined immunohistochemically in frozen sections . ^^^ No relationship between EGFR and ER expression was recognized . ^^^ However , a case of papillary carcinoma with strong ER immunoreactive intensity expressed weak EGFR intensity , suggesting an inverse relation in immunoreaction between EGFR and ER in papillary carcinoma . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The effects of this ectopic ER expression were studied on three endogenous E 2 responsive genes , prolactin ( PRL ) , progesterone receptor ( PR ) , and epidermal growth factor receptor ( EGFR ) . ^^^ Our studies paint a complicated picture of E 2 regulation of endogenous genes : the activation of the PR gene may only require the presence of E 2 and ER , whereas EGFR and PRL genes require factors in addition to ER for basal as well as E 2 regulated expression . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In addition , alkaline phosphatase fused to the extracellular and transmembrane domains of epidermal growth factor receptor was transported to the plasma membrane in COS 1 cells , which establishes that alkaline phosphatase as a cytoplasmic domain does not prevent transport from the ER . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We did not find a significant accumulation of EGF R positive tissues in the group of ER negative tumours ( 11 / 28 respectively 16 / 32 ) . ^^^ Nevertheless most ER positive tumours showed a negative EGF R receptor score ( 32 / 52 ; 27 / 48 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR mRNA expression was measured in 106 patients and was inversely correlated with ER status ( P = 0 . 018 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In this study epidermal growth factor receptor ( EGF R ) , estrogen receptor ( ER ) , and progesterone receptor ( PR ) status was evaluated in 326 primary breast carcinomas . ^^^ Nineteen percent of samples were EGF R positive , 63 % were positive for ER , and 54 % for PR . ^^^ Sixty nine percent of EGF R negative tumors were ER positive and 51 % were positive for ER as well as PR . ^^^ In our study we reaffirmed the negative correlation between steroid receptor status and the overexpression of EGF R ; furthermore the combination of EGF R+ and ER tumors was observed more often in histological high risk tumors . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
This behaviour can be divided into two groups ; the first containing the genes , pLIV 1 and pLIV 2 ( pS 2 ) , which both show a significant association with ER status ( P = 0 . 001 ) and a corresponding inverse relationship with epidermal growth factor receptors ( EGFR ) ( P = 0 . 01 and P = 0 . 08 , respectively ) . ^^^ Unlike pLIV 1 and pS 2 , pSYD 3 and pSYD 8 belong to a group of genes which are expressed in the majority of tumours regardless of ER and EGFR status . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We have compared the tumour cytosolic cathepsin D level with EGFr and oestrogen receptor ( ER ) levels and the axillary node status of 131 patients with operable breast cancer , to see if EGFr and cathepsin D are co regulated . ^^^ No correlation was found between the level of cathepsin D and EGFr , ER or nodal status . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
High tumor levels of ER , PgR , AR and pS 2 predict a relatively good response to endocrine therapy , while EGF R positively , HER2 / neu positivity , aneuploidy , high proliferation indices and possibly high uPA levels indicate a high chance of poor response to endocrine therapy in metastatic breast cancer . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The longest mean survival time occurred in patients with negative EGFr and 5 status and positive hormone receptor ( ER and PR ) status . ^^^ The shortest mean survival time occurred in patients with positive EGFr and 5 and negative ER and PR status . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The expression of mRNAs for epidermal growth factor ( EGF ) , transforming growth factor alpha ( TGF alpha ) , EGFR , platelet derived growth factor ( PDGF ) A and B chain , PDGF receptor ( PDGFR ) , transforming growth factor beta ( TGF beta ) , erbB 2 and estrogen receptor ( ER ) genes was first examined in 6 human esophageal carcinoma cell lines , 6 xenoplanted and 15 surgically resected esophageal carcinomas . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Positivity of epidermal growth factor receptor ( EGFR ) was 60 % in ER negative and PR negative breast cancer and 30 % in ER positive and PR positive breast cancer . ^^^ The significance of TGF alpha as an autocrine growth factor appears to be more important in ER negative and PR negative breast cancer with high EGFR positivity than in ER positive and PR positive breast cancer with low EGFR positivity . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We have performed immunocytochemical analysis of the expression of epidermal growth factor receptor ( EGF R or proto oncogen c erbB 1 ) and estrogen receptor ( ER ) in 70 cases of human breast carcinoma . ^^^ We found an inverse relationship between the expression of EGF R and ER ( Kendall ' s tau b = 0 . 1997 , P < 0 . 03 ) , which prompts us to conclude that ER ( ) breast carcinomas may grow in a hormone independent manner through the over expression of the proto oncogene c erbB 1 , which is the receptor for epidermal ( EGF ) and alpha transforming ( TGFalfa ) growth factors . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Regression analysis of EGFR versus ER and PR levels confirmed an inverse relationship between EGFR and ER ( p = 0 . 022 ) as well as between EGFR and PR ( p = 0 . 024 ) . ^^^ Univariate analysis of the EGFR data stratified according to steroid hormone receptor status showed EGFR to be negatively associated with ER and PR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
After a median follow up period of 57 months , multivariate analysis suggested that EGF r expression was associated with increases in risk for both relapse and death from breast cancer , even after adjusting for oestrogen receptor ( ER ) concentration , tumour size and the number of axillary nodes involved . ^^^ Patients with ER positive tumours , which also expressed EGF r , had increases in risk for both relapse and death from breast cancer compared with tumours without EGF r . ^^^ Expression of EGF r was not a predictor of poor prognosis in either node negative or ER negative subgroups of patients . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Nuclear estrogen receptor ( ER ) binding and ER mRNA levels were similar in wild type and mutant cell lines , whereas epidermal growth factor receptor mRNA levels were significantly decreased in the variant BaPr T47D cells . 17beta Estradiol induced proliferation of both wild type and BaPr T47D cells , and TCDD inhibited this response but did not down regulate nuclear ER levels . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Progesterone ( PR ) , estradiol ( ER ) , and EGF receptor ( EGFR ) content was measured by radioligand competitive methods in surgically excised tumors from 26 patients with primary endometrial cancer . ^^^ No significant correlations were revealed between EGFR and ER / PR status or concentration . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Some of these compounds were shown to block PTK activity in vitro , but also efficiently to inhibit EGF stimulated DNA synthesis in ER 22 cells ( CCL 39 hamster fibroblasts transfected with EGF R ) with IC 50 values in the range 1 10 microM . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
On the other hand , axillary lymph node status ( P = 0 . 95 ) , histological type ( P = 0 . 60 ) , tumor size ( P = 0 . 87 ) , DNA index ( P = 0 . 56 ) , S phase ( P = 0 . 80 ) , mitotic index ( P = 0 . 72 ) , or estrogen ( ER ) and progesterone receptor ( PR ) content ( P = 0 . 45 ) did not show any statistical correlation with the EGFR expression . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Expression of the E receptor ( ER ) , P receptor ( PR ) , epidermal growth factor receptor ( EGFR ) and insulin like growth factor ( IGF 1 ) was equivalent under E or P dominance . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In summary , our studies demonstrate that SEC and KC contain significant amounts of high affinity ER and that ER pathways may modulate some activities of the SEC and KC , but that ER EGFR interactions may be different in these cells from hepatocytes . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
OBJECTIVE : We tested whether immunohistochemical detection of oestrogen and progesterone receptor ( ER , PR ) , the oestrogen dependent protein pS 2 , the growth hormone receptors p 185neu and EGF R , the tumour suppressor protein p 53 , the tissue proteases Cathepsin D and Urokinase , and the proliferation marker PCNA are of prognostic relevance in breast cancer patients . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The gene , designated scFv R1R , was introduced into EGFR transformed NIH3T3 cells via retroviral infection . scFv R1R was directed to the lumen of the endoplasmic reticulum ( ER ) where it bound the extracellular domain of the receptor inhibiting its appearance on the plasma membrane . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The concentrations of receptors for epidermal growth factor ( EGF R ) , oestrogen ( ER ) and progesterone ( PR ) were measured in 108 samples from canine mammary tumours and 132 samples of normal mammary tissue removed surgically from 84 bitches . ^^^ A significant direct correlation ( P = 0 . 002 ) was observed between the concentrations of ER and EGF R only in malignant tumours . ^^^ The direct correlation between the concentrations of EGF R and ER in malignant tumours could be related to an oestrogen dependent expression of EGF R or to a similar pattern of regulation of the receptors . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Mammary adenocarcinomas induces in female Sprague Dawley rats by three intraperitoneal injections of N nitroso N methylurea were studied in order to characterize their estrogen ( ER ) , progesterone ( PgR ) , prolactin ( PRLR ) and epidermal growth factor ( EGFR ) receptors . ^^^ All samples evaluated showed the presence of ER and PgR in the cytosol fraction and PRLR amd EGFR in the membrane fraction . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Human breast cancer is often characterized by a progression to an ER ( estrogen receptor ) negative , estrogen independent , antiestrogen resistant , EGFR ( epidermal growth factor receptor ) positive , and highly metastatic phenotype . ^^^ By constructing stable and complete human human somatic cell fusions between a highly metastatic , undifferentiated , ER negative line of melanoma lineage and the estrogen dependent , ER positive MCF 7 line , this study produced hybrids that were ER negative , highly expressive of EGFR , estrogen independent , estrogen unresponsive , fully tumorigenic , and highly metastatic . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In breast cancer , epidermal growth factor ( EGF ) receptor ( EGFR ) expression is inversely correlated with expression of estrogen receptor ( ER ) and predicts the prognosis and failure of endocrine therapy . ^^^ We report here , for the first time , that in ER positive breast cancer cell lines , MCF 7 , T47D , and BT 474 , 17 beta estradiol ( E 2 ) transiently induced EGFR messenger RNA ( mRNA ) levels 2 to 3 fold ; this induction was prevented by the presence of the antiestrogen ICI 164 , 384 and was also reflected in the level of EGFR protein . ^^^ Up regulation of EGFR mRNA is most likely due to a direct effect of ER on the EGFR gene , with no involvement of protein synthesis , as it was not inhibited in the presence of cycloheximide ; however , the subsequent down regulation of EGFR required de novo protein synthesis . ^^^ Additionally , sequencing of the EGFR promoter revealed putative imperfect estrogen responsive elements that were capable of binding human ER . ^^^ The transient nature of EGFR induction by E 2 , with a rapid return to a basal level that is dependent on protein synthesis , suggests that breast cancer cells possess active mechanisms to maintain low levels of EGFR expression in the presence of estrogen and a functional ER . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The growth fraction ( MIB 1 ) , oestrogen receptor ( ER ) , progesterone receptor ( PR ) , p 53 mutant protein , c erbB 2 , epidermal growth factor receptor ( EGFR ) , NCRC11 / epithelial membrane antigen ( EMA ) and DNA plopidy were examined . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The expression of c erbB 2 oncoprotein , epidermal growth factor receptor ( EGFR ) and estrogen receptor ( ER ) was evaluated by the immunoperoxidase technique ( PAP ) in ductal breast carcinomas . ^^^ Stratifying of patients on the basis of c erbB 2 , EGFR and ER status indicated that the combination of c erbB 2 overexpression accompanied by high EGFR value and undetectable ER , identified poorly differentiated tumors and patients with high incidence of axillary lymph node metastases , while high EGFR expression and negative c erbB 2 staining was connected only with poor tumor grade . ^^^ Our results indicate that the comparison of c erbB 2 , EGFR and ER status seems to be a powerful tool in discriminating breast carcinomas with different biological phenotypes . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In human breast cancer cell lines , an inverse relationship exists between the basal levels of oestrogen receptor ( ER ) and epidermal growth factor receptor ( EGF R ) gene expression . ^^^ In addition , the tumour promoting phorbol ester 12 O tetradecanoyl phorbol 13 acetate ( TPA ) inhibits ER and stimulates EGF R expression in MCF 7 breast cancer cells . ^^^ This study aimed to define further the potential mechanisms involved in the modulation of ER and EGF R gene expression by TPA . ^^^ The TPA induced decrease in ER mRNA was unaffected by the simultaneous administration of the protein synthesis inhibitor cycloheximide , whereas the increase in EGF R mRNA was inhibited by co incubation with cycloheximide . ^^^ These data indicate a requirement for continuing protein synthesis for the TPA effect on EGF R but not on ER mRNA levels . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The prognostic value of epidermal growth factor receptor ( EGFR ) expression and its biological role in estrogen receptor positive ( ER+ ) and ER negative ( ER ) primary breast cancer is controversial . ^^^ In this study , distributions of ER , progesterone receptor and EGFR have been established using immunohistochemistry in both primary breast tumors and their matched axillary lymph node metastases of 26 patients or their matched distant metastases of 2 patients . ^^^ Expression of ER and EGFR was inverse regarding the individual tumor cells in both primary tumors and metastases . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Results were integrated with the oestrogen and progesterone receptor ( ER and PGR ) status , Ki 67 marking , and epidermal growth factor receptor ( EGFr ) expression . ^^^ Group 3 consistently comprised tumours with the highest Ki 67 levels , EGFr positivity , and ER PGR negative status . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
There was an inverse relationship between EGF R values and those for the estrogen receptor ( ER ) , progesterone receptor and pS 2 protein as well as menopausal status . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Expression of oestrogen receptor ( ER ) , epidermal growth factor receptor ( EGFR ) and transforming growth factor alpha ( TGF alpha ) proteins was assessed by immunocytochemistry on primary breast cancer specimens obtained before and following short term ( 7 day ) presurgical exposure to pure anti oestrogen ( 7 alpha [ 9 ( 4 , 4 , 5 , 5 , 5 pentafluoropentylsulphinyl ) nonyl ] estra 1 , 3 , 5 , ( 10 ) triene 3 , 17 beta diol , ICI 182780 ) treatment and compared with no treatment controls . ^^^ Effects of ICI 182780 ( 10 ( 7 ) M ) on MCF 7 breast cancer cell ER , EGFR and TGF alpha expression were also examined over 14 days . ^^^ ICI 182780 suppresses ER without a concomitant rise in either EGFR or TGF alpha . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The effect of gp 30 on the concentration of ER was independent of serum in the media and was not inhibited by an epidermal growth factor receptor blocking antibody . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The pattern and extent of reactivity has been correlated with clinicopathological data including tumour type , grade and lymph node status and with other prognostic parameters including oestrogen receptor ( ER ) status , expression of c erbB 2 , pS 2 protein and epidermal growth factor receptor ( EGFR ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In 215 patients with operable breast cancer ( T 1 T3 , N 0 1 , M 0 ) and no other or previous cancer , presenting to a single breast unit , sufficient tumour was available for the prospective determination of four putative biochemical markers of prognosis : oestrogen receptor ( ER ) activity , cathepsin D ( cath D ) , epidermal growth factor receptor ( EGFR ) activity and cyclic AMP binding proteins ( c AMP b ) . ^^^ There were significant inter relationships between ER and EGFR ( r = 0 . 26 ) , c AMP b and cath D ( r = +0 . 32 ) and ER and c AMP b ( r = +0 . 14 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The present study attempts to clarify the specific contribution of cathepsin D ( CD ) and pS 2 to the progression of breast cancer ( BC ) by examining the relationship between these two factors and TNM status , tumour grade , estradiol receptors ( ER ) and the prognosis factors epidermal growth factor receptor ( EGFR ) and neu amplification in a group of 270 BC patients . ^^^ EGFR was determined in membrane tumour preparations obtained from ER cytosols by a two point radiometric saturation assay . ^^^ CD is basically related to bad prognosis factors and has a direct correlation with tumour size ( P = 0 . 025 ) and EGFR content ( P = 0 . 007 ) and is associated with the presence of metastases ( P = 0 . 000 ) . pS 2 is mostly related to good prognosis factors and showed an inverse correlation with the Scarff Bloom Index ( P = 0 . 011 ) and a direct correlation with ER content ( P = 0 . 014 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
ER ( + ) gastric tumors were closely associated with the intestinal type ( P < 0 . 01 ) , whereas EGF R ( + ) tumors were significantly correlated with poor differentiation status and ER ( + ) expression ( P < 0 . 01 ) . ^^^ Of EGF R ( + ) tumors , 85 % were also ER ( + ) . ^^^ EGF R and ER co expression was demonstrated in 17 tumors ( 32 % of the group ) . ^^^ CONCLUSIONS : ER and EGF R co expression indicates that a functional interaction between estrogens and EGF may exist in gastric cancer and that when such an interaction becomes operative , it may lead to dedifferentiation and increased tumor growth . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In a prospective pilot study , we performed breast fine needle aspirations ( FNAs ) on 213 high risk and 30 low risk women and analyzed these aspirates for cytologic changes and biomarker abnormalities of aneuploidy and overexpressed estrogen receptor ( ER ) , epidermal growth factor receptor ( EGFR ) , p 53 and HER 2 / neu . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
On examining the breast tumours , alpha JSB 1 showed a positive association with c erbB 2 ( P = 0 . 003 ) , c myc ( P = 0 . 0004 ) and c jun ( P = 0 . 02 ) but not ER or EGF R expression . alpha GSTpi showed a positive association with c erbB 2 ( P = 0 . 03 ) and c myc ( P = 0 . 0004 ) but not ER , EGF R or c jun . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
These studies have suggested a `` cross talk ' ' model in which EGF receptor ( EGF R ) signaling results in activation of nuclear estrogen receptor ( ER ) and its target genes in an estrogen independent manner . ^^^ The EGF R levels , autophosphorylation , and c fos induction were observed at equivalent levels in both genotypes indicating that removal of ER did not disrupt the EGF responses . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The estrogen receptor ( ER ) , epidermal growth factor receptor , and vascular density were analyzed in 64 primary breast cancers . ^^^ High VEGF expression correlated with poor prognosis in univariate analysis ( P = 0 . 03 ) , as did ER and epidermal growth factor receptor expression . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
These E1A results were correlated with ICA , EGFR and ER were negatively correlated . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor receptor ( EGFR ) , progesterone receptor ( PR ) and estrogen receptor ( ER ) status were analyzed in 108 primary ovarian epithelial carcinoma specimens . ^^^ Receptor concentration was determined with radioligand binding assays . 61 % of the carcinomas investigated were positive for EGFR , 29 % for PR and 57 % for ER . ^^^ Epidermal growth factor receptor ( EGFR ) , progesterone receptor ( PR ) and estrogen receptor ( ER ) status were analyzed in 108 primary ovarian epithelial carcinoma specimens . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
To better understand how EGF R and estrogen receptor ( ER ) operate together to stimulate breast cancer cell growth , we analyzed the % S in the two groups of ER negative ( ER ) and ER positive ( ER+ ) tumors , stratifying the patients on the basis of EGF R tumor positivity . ^^^ Here breast tumor proliferation activity seems mainly to be induced by the stimulus of EGF R , the % S values of the EGF R negative tumors in the ER and ER+ groups being 6 . 1 and 6 . 9 % , respectively . ^^^ Instead , the median % S of EGF R positive tumors was 10 % in the ER class and 14 % in the ER+ group . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
On the same specimens steroid hormone receptors ( ER and PR ) were measured in the cytosol fraction using Abbott ELIZA assays , c erbB 2 and EGFR were determined in the membrane fraction and mutant p 53 protein in the nuclear fraction by Oncogene Science ELISA procedures . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The correlation between the presence of ER / PGR sites , high level of TGF alpha / EGF mRNA and EGF R occupancy by endogenous ligand is in favour of ER mediated control of TGF alpha and EGF production . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Prognostic significance of c erbB 2 / neu amplification and epidermal growth factor receptor ( EGFR ) in primary breast cancer and their relation to estradiol receptor ( ER ) status . ^^^ The aim of this study is to evaluate the prognostic significance of c erbB 2 / neu amplification and epidermal growth factor receptor ( EGFR ) expression in primary breast cancer ( BC ) and their prognostic implications when combined with estradiol receptor ( ER ) status . ^^^ Neu , EGFR , estradiol and progesterone receptors ( ER and PR ) had a marked influence on disease free survival ( DFS ) in univariate analysis . ^^^ The EGFR + / neu / phenotype represented a sub group with an even worse prognosis with respect to DFS ( p = 0 . 0034 ) as well as EGFR + / ER tumors ( p = 0 . 005 ) . ^^^ C erbB 2 / neu , EGFR , histological grade , pN , pT and ER were subjected to a Cox multivariate regression analysis : neu was the most important parameter in predicting recurrence , and EGFR was a significant predictor for OS . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
An investigation of the effects of phenotypic expression of estrogen receptors ( ER ) , the progesterone receptors , c erbB 2 oncoprotein and epidermal growth factor receptors ( EGFR ) on the capacity of HMEC cells to grow in vitro as monolayers showed that expression of ER and EGFR is required for controlling tumor proliferative activity in vitro . ^^^ Expression of ER protein made the growth of HMEC cells more difficult , while expression of EGFR protein made their growth in vitro easier . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Responder patients displayed a significant decrease in ER levels ( p = 0 . 015 ) , whereas in non responders there was an increase in PCNA ( p = 0 . 008 ) and EGFr expression ( p = 0 . 002 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In human breast cancer , progression to a more malignant phenotype is often accompanied by decreased expression of estrogen receptor ( ER ) and increased expression of epidermal growth factor receptor ( EGFR ) . ^^^ Higher levels of this receptor tyrosine kinase are found in tumors lacking ER , and a quantitative , inverse relationship exists between the level of ER and EGFR mRNA in human breast cell lines . ^^^ Antisense ER ( ASER ) RNA was used to evaluate the consequence of decreased ER expression in breast cancer cells , specifically to determine whether ER is involved in the regulation of EGFR gene expression . ^^^ ER positive MCF 7 human breast cancer cells were transfected with ASER , and clones constitutively expressing ASER RNA had decreased ER and up to a 3 fold increase in the expression of EGFR mRNA . ^^^ These data support the hypothesis that the level of expression of ER specifically influences the expression of EGFR in human breast cancer cells and provides a potential link between loss of steroid sensitivity and the acquisition of autonomous growth . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
On the same specimens steroid hormone receptors ( ER and PR ) were measured in cytosol fraction using Abbott ELIZA assays , c erbB 2 and EGFR were determined in the tissue homogenate and mutant p 53 protein in the nuclear fraction by Oncogene Science ELISA procedures . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The present work showed also an inverse correlation between EGFR and Estrogen Receptor ( ER ) level in the malignant tissues . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In addition there was an inverse association between EGFR and ER concentration , but no association between EGFR and PR . ^^^ CONCLUSIONS : Our study revealed associations between EGFR , ER , Ki 67 and ploidy . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We immunohistochemically investigated expression patterns of these growth factors and EGFR along with estrogen receptor ( ER ) status in 36 breast carcinomas ( 21 invasive ductal , 11 invasive lobular , 4 noninvasive ductal ) and compared the results with those found in 10 fibroadenomas . ^^^ EGFR expression significantly correlates with negative ER status in breast carcinoma . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
A time dependent stimulation of epidermal growth factor receptor overexpressing clone 22 ( ER 22 ) cells by epidermal growth factor resulted in an immediate increase of the Grb2 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In contrast , TC 48 D182A formed a complex in the ER with pTyr epidermal growth factor receptor ( EGFR ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In this paper we review the morphological and biological features of progestin induced tumors , such as estrogen receptor ( ER ) and progesterone receptor ( PR ) patterns of expression , hormone dependence and epidermal growth factor receptors ( EGF R ) we also examine our data concerning the systemic effects of medroxyprogesterone acetate ( MPA ) as regards its stimulating EGF synthesis in salivary glands and its subsequent increase in serum . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In a prospective pilot study , we performed breast fine needle aspirations ( FNAs ) on 224 high risk and 30 low risk women and analyzed these aspirates for cytologic changes and biomarker abnormalities of aneuploidy and overexpressed estrogen receptor ( ER ) , epidermal growth factor receptor ( EGFR ) , p 53 and HER 2 / neu . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The median of pO 2 values and the percentage of hypoxic areas ( pO 2 < 10 mmHg ) were calculated and correlated with the histological type , grading , ER , PR , and the expression of Ki 67 , p 53 , EGFR , pS 2 , and c erb B 2 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Cells expressed mRNAs for estrogen receptor ( ER ) , TGF beta , EGF R , PRL R , IL 1 alpha , and IL 6 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We examined c erbB 3 and c erbB 4 mRNA expression in 47 primary breast cancer samples by simultaneous RT PCR and have investigated correlations between these parameters and the expression of both ER and EGFR mRNA and protein as measured by RT PCR and ICA and with Ki 67 immunostaining . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Concentrations of receptors of insulin like growth factor 1 ( IGF 1 R ) , epithelial growth factor ( EGF R ) , oestrogen ( ER ) , and progesterone ( PR ) in the adenomyosis foci , endometrium and myometrium of women during the menstrual cycle were studied . ^^^ The findings show that changes in regulation of the concentrations of IGF 1 R , EGF R , ER and PR in the uterus of women during menstrual cycles are related to the tissular localization of these receptors . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Using immunocytochemistry , tumors were immunostained for overexpression of c erb B 2 , epidermal growth factor receptor ( EGFR ) , p 53 , and expression of the Ki 67 defined antigen ( a marker of cellular proliferation ) , tumor necrosis factor alpha ( TNFalpha ) , estrogen receptor ( ER ) , progesterone receptor ( PR ) , and P glycoprotein ( P 170 , a marker of multidrug resistance ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
At 50 nM , the peptidimer blocks selectively Grb 2 Sos complexation in ER 22 ( CCL 39 fibroblasts overexpressing epidermal growth factor receptor ) cellular extracts . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The expression of epidermal growth factor receptor ( EGFR ) mRNA and protein has been determined in a group of breast carcinomas and compared to oestrogen and progesterone receptor ( ER , PgR ) status , as well as pathological features . ^^^ EGFR protein was detected in 36 % and these tumours had a strong correlation to lack of ER and high histological grade . ^^^ The ER / PgR carcinomas showed the full range of EGFR mRNA labelling . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : We have measured bFGF levels in 149 human primary breast carcinomas and assessed the findings in relation to microvessel density , oestrogen receptor ( ER ) and epidermal growth factor receptor ( EGFR ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The antibodies used were against the receptors for epidermal growth factor ( EGF R ) , estrogen receptor ( ER ) , and progesterone receptor ( PR ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Endometrial specimens of 34 ( 25 premenopausal and 9 postmenopausal ) breast cancer patients receiving tamoxifen were immunohistochemically examined using estrogen receptor ( ER ) , progesterone receptor ( PR ) , Ki 67 , and epidermal growth factor receptor ( EGFR ) antibodies . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The purpose of this study was to investigate whether tamoxifen ( TAM ) treatment causes a downregulation of estrogen receptor ( ER ) and whether TAM induces epidermal growth factor receptor 1 ( EGFR ) . ^^^ We investigated the expression of ER and EGFR after the treatment of TAM in MCF 7 tumors grown in athymic mice under high and low estrogen environments . ^^^ Both ER and EGFR protein levels in the tumors of the estrogen depleted mice were higher than in the estrogen completed mice . ^^^ Expression of ER and EGFR protein was increased by TAM in the estrogen completed mice , however it was decreased by TAM in the estrogen depleted mice . ^^^ Changes of ER and EGFR protein levels were similar in all treatments . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Although a significant inverse correlation was found between EGF R status , and ER or PR status , quantitative content of EGF R did not correlate either with quantitative ER , or PR levels . ^^^ Our results emphasized the usefulness of quantitative receptor determination suggesting the relative stability of EGF R content during the clinical course of breast cancer , its independence from ER , its significant predictive and weak prognostic values , and a possible correlation with the aggressiveness of the disease , and response to non endocrine treatments . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
These two variables were also compared with the pathological features of the tumours , as well as oestrogen receptor ( ER ) , epidermal growth factor receptor ( EGFR ) , microvessel density , macrophage index , and survival . ^^^ In the EGFR negative group of cases ( n=56 ) , positive associations were observed between VEGF expression and macrophage index ( p=0 . 005 ) , ER ( p=0 . 05 ) , p 53 ( p=0 . 006 ) , tumour grade ( p=0 . 02 ) , and tumour necrosis ( p=0 . 03 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Therefore , the patterns of expression of the receptors , epidermal growth factor receptor ( EGF R ) , c erbB 2 , c erbB 3 , c erbB 4 , and one of the erbB ligands , heregulin ( HRG ) , were examined in normal and malignant breast cell lines and compared with expression of oestrogen receptor ( ER ) , a classical indicator of good prognosis . ^^^ There was an inverse correlation between ER and EGF R mRNA levels , as previously described , but no correlation between either of these receptors and c erbB 2 . c erbB 3 expression was positively correlated with ER . ^^^ Expression of antisense ER resulted in increased EGF R mRNA , demonstrating a functional link between the expression of these 2 genes , however , there was no significant change in c erbB 2 or c erbB 3 mRNA , suggesting that ER is not directly involved in control of expression of these genes . ^^^ In summary , the levels of expression of c erbB 1 , 2 , 3 , and 4 varied in this series of breast cell lines , and the pattern of expression and the relationship of each growth factor receptor to the expression of ER was quite distinct . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The expression of estrogen receptors ( ER ) and epidermal growth factor receptors ( EGFR ) in the gastrointestinal tract might therefore result in functional cross talk between estrogens and EGF . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
While the individual ER contents in endocrine sensitive and endocrine resistant tumors overlapped , the EGF R ranges were different : no one endocrine sensitive tumor exceeded the EGF R content of 26 fmol / mg , thus suggesting the EGF R cut off point of endocrine sensitivity . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In a prospective random fine needle aspiration ( FNA ) study of women at high risk of development of breast cancer , we previously demonstrated that cytologic evidence of epithelial hyperplasia with or without atypia , as well as abnormalities of several cellular biomarkers ( DNA ploidy ; immunocytochemical expression of p 53 , EGFR , ER , and / or Her 2 / neu ) , were more prevalent in high risk women than in low risk controls . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
No correlation was shown between drug sensitivity and factors , i . e . pathological type , clinical type , expression of estrogen receptor ( ER ) and epithelium growth factor receptor ( EGFR ) for breast carcinoma ( P > 0 . 05 ) . 3 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The epidermal growth factor ( EGF ) family of receptors ( EGFR ) is overproduced in estrogen receptor ( ER ) negative ( ) breast cancer cells . ^^^ An inverse correlation of the level of EGFR and ER is observed between ER and ER positive ( + ) breast cancer cells . ^^^ A comparative study with EGFR overproducing ER and low level producing ER+ breast cancer cells suggests that EGF is a major growth stimulating factor for ER cells . ^^^ The basal level of active NF kappaB in ER cells is elevated by EGF and inhibited by anti EGFR antibody ( EGFR Ab ) , thus qualifying EGF as a NF kappaB activation factor . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Likewise , cytosolic levels of estrogen receptors ( ER ) , progesterone receptors ( PR ) , pS 2 , cathepsin D and tissue . type plasminogen activators ( t PA ) have been dossified , as well as those of epidermal growth factor receptor ( EGFR ) at cell surfaces . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Immunoreactivity of EGFR was related to clinicopathological features , estrogen receptor ( ER ) and progesterone receptor ( PR ) status , and patient outcome . ^^^ EGFR expression was not related to the ER / PR immunostaining in endometrial carcinomas . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
RESULTS : ER / pS2+ IDC presented higher ( p < 0 , 05 ) PR , t PA and HA cytosol level , as well as lower EGFR concentrations , S phase > 7 % and S phase > 14 % and lower N+ > 10 percentages and aneuploidy . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The results were compared with those of the estrogen ( ER ) and progesterone ( PR ) receptors , pS 2 , tissue type plasminogen activator ( t PA ) , cathepsin D , epidermal growth factor receptor ( EGFR ) and c erbB2 / neu oncoprotein concentrations . ^^^ Furthermore , CD44v6 positive IDC showed significantly higher concentrations of ER , PR and cathepsin D and lower ( p : 0 . 051 ) concentrations of EGFR when compared to their CD44v6 negative counterparts . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR and c erbB 2 were inversely correlated to the presence of estradiol receptors ( ER ) and progesterone receptors ( PgR ) , and positively correlated to the histoprognostic grading ( HPG ) . ^^^ EGFR was inversely related ( chi 2 test ) to the presence of ER and PgR , and positively associated with HPG . ^^^ The expression level of EGFR and c erbB 2 was significantly higher in ER and PgR negative tumors compared with ER and PgR positive tumors ( Student ' s t test ) , and in tumors with higher grade compared with tumors with lower grade . ^^^ In overall survival studies , Cox univariate analyses showed prognostic values of EGFR [ > or = median ; P = 0 . 026 ; risk ratio ( RR ) , 1 . 6 ] , c erbB 3 ( > or = median ; P = 0 . 0093 ; RR , 0 . 58 ) , c erbB 4 ( > or = median ; P = 0 . 0024 ; RR , 0 . 52 ) , HPG , node involvement , tumor diameter , ER , and PgR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
METHODS : To determine the prognostic value of the urokinase plasminogen activation system , contents of uPA , uPAR , and PAI 1 were measured in extracts of endometrial cancer tissue using ELISAs . uPA , uPAR , and PAI 1 levels were determined in 91 , 54 , and 92 extracts , respectively , and correlated with tumor histology , stage , grade , lymph node involvement , prevalence of metastasis , and recurrence as well as with estrogen ( ER ) , progesterone ( PR ) , epidermal growth factor ( EGFR ) receptor and HER 2 / neu contents . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
TCDD elevates erbB 2 expression and signaling in T47D cells by reversing serum potentiation of estrogen receptor activity , independent of estrogen levels and enhanced ER down regulation . 2 , 3 , 7 , 8 Tetrachlorodibenzo p dioxin ( TCDD ) induced erbB 2 and erbB 3 in estrogen receptor ( ER ) positive T47D ( T47D+ ) cells , but substantially slower than the direct induction of CYP1A1 or CYP1B1 . ^^^ Similar maximum erbB levels were observed in ER T47D cells or in T47D+ cells cultured in estrogen ( E 2 ) free , defined media ( SFM ) or serum media with anti estrogen ICI 182 , 780 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Hormone independent human breast cancer is characterized by estrogen receptor ( ER ) loss and the acquisition of high epidermal growth factor receptor ( EGFR ) levels . ^^^ Despite the tendency for an inverse correlation between EGFR and ER , EGFR is a strong prognostic indicator for poor survival rate independent of ER status suggesting that EGFR overexpression is an important step in the progression to estrogen independence . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Tissues from 100 cases of breast cancer were analysed immunohistochemically for the presence of adrenocorticotropic hormone ( ACTH ) or ACTH like peptides and expression of c erbB 2 oncoprotein , epidermal growth factor receptor ( EGF R ) as well as oestrogen receptor ( ER ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In order to study the association of histological grade ( HG ) with specific clinical and biological parameters which may influence the clinical behavior of infiltrating ductal carcinomas of the breast ( IDC ) , we analyzed in 229 tissue samples the cytosolic concentrations of estrogen receptor ( ER ) , progesterone receptor ( PR ) , pS 2 , cathepsin D , hyaluronic acid ( HA ) and tissue type plasminogen activator ( t PA ) , as well as those of the erbB 2 oncoprotein , epidermal growth factor receptor ( EGFR ) , HA , CD44v5 and CD44v6 in the cell membrane fraction . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
METHODS : Estrogen receptor ( ER ) , progestoren receptor ( PR ) , epidermal growth factor receptor ( EGFR ) and growth hormone ( GH ) were determined by immunohistochemical SABC method in 30 cases of adenomyosis ( group 1 ) and another 24 cases of adenomyosis accompanying leiomyoma ( group 2 ) ; ER , PR were determined with the same methods in 24 cases of adenomyosis who had been treated with methyltestosterone before operation ( group 3 ) . ^^^ RESULTS : ( 1 ) Both the eutopic and ectopic endometrium in group 1 showed ER , PR , EGFR , GH positive . ^^^ The strong positive rate of ER , PR in the eutopic endometrium were significantly higher than that in the ectopic ( P < 0 . 05 ) . ( 2 ) ER , PR , EGFR , GH in the eutopic and ectopic endometrium in group 1 and group 2 all showed positive but no significant differences ( P > 0 . 05 ) . ( 3 ) Both ER and PR showed positive in group 1 , 2 and group 3 . ^^^ CONCLUSIONS : ER , PR , EGFR , GH all expressed in the eutopic and ectopic endometrium . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
A significant inverse relationship was found between estradiol receptors ( ER ) and EGFR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The presence of the 10 allele of ER gene significantly correlated with the overexpression of the erbB 2 and EGFR oncogenes . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Thus , NF kappaB is a potential target for therapy of EGFR family receptor overexpressing ER breast cancers . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
To evaluate mechanisms of cell proliferation in the fetal female rat reproductive tract , diethylstilbestrol ( DES ) effects on cell division and estrogen receptor ( ER ) , epidermal growth factor ( EGF ) and EGF receptor ( EGF R ) expressions were determined from gestational day ( GD ) 15 . 5 to 21 . 5 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Differences in response rates between letrozole and tamoxifen were most marked for tumors that were positive for ErbB 1 and / or ErbB 2 and ER ( 88 % 5 21 % , P = . 0004 ) . ^^^ This suggests that ErbB 1 and ErbB 2 signaling through ER is ligand dependent and that the growth promoting effects of these receptor tyrosine kinases on ER+ breast cancer can be inhibited by potent estrogen deprivation therapy . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor receptor ( EGFR ) is a transmembrane receptor whose overexpression in breast cancer predicts for poor prognosis and is inversely correlated with expression of estrogen receptor ( ER ) . ^^^ Epidermal growth factor receptor ( EGFR ) is a transmembrane receptor whose overexpression in breast cancer predicts for poor prognosis and is inversely correlated with expression of estrogen receptor ( ER ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
However , ER positive breast tumors overexpressing EGF R and / or HER 2 ( Erb B 2 ) are resistant to endocrine therapy . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor ( EGF ) and estradiol ( E 2 ) are important mitogens in breast epithelial cells , and expression of epidermal growth factor receptor ( EGFR ) and estrogen receptor ( ER ) is often inversely correlated in human breast cancer cells . ^^^ Moreover , we found that EGF induced signalling also could be reduced by E 2 in S3B cells , suggesting a general mechanism of action by E 2 in cells concomitantly expressing ER and EGFR . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The current article examines the modulation of growth factor networks during endocrine response , and presents in vitro and clinical evidence that epidermal growth factor receptor signalling , maintained in either an ER dependent or independent manner , is critical to anti hormonal resistant breast cancer cell growth . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Immunostaining for epidermal growth factor receptor ( EGFR ) , c erbB 2 , c erbB 3 , c erbB 4 , ER , and PR was performed in 107 cases of primary breast carcinomas from Anyang , China . ^^^ No significant associations between the expressions of EGFR , c erbB 3 , c erbB 4 , ER , and PR and overall survival were detected . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Estrogen receptors ( ER ) , progesterone receptors ( PR ) , pS 2 , cathepsin D , tissue type plasminogen activator ( t PA ) and hyaluronic acid ( HA ) cytosolic concentrations were analyzed in addition to those of the epidermal growth factor receptor ( EGFR ) , HA and erbB2 / neuoncoprotein in cell surfaces . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In this study we describe detailed immunohistochemical analysis of nuclear and cytoplasmic STATs 1 and 3 expression in primary breast carcinomas and correlate this with EGFR , HER 2 , p 53 , ER , PR , p21 / waf1 , Bcl XL and Ki 67 expression . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
However , the estrogen receptor ( ER ) content remained similar in tumors from both groups , at least at the protein level , and so did the expression of IGF 1 , IGF 2 , insulin receptor substrate 1 ( IRS 1 ) , IRS 2 and epidermal growth factor receptor . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Expressions of EGFR , ER and PR were strong in all cases , but IGFR was negative in almost all cases . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Of the tumors 48 % were immunoreactive for EGFR , 63 % for c erbB 2 , 78 % for c erbB 3 , 95 % for c erbB 4 , 88 % for estrogen receptor ( ER ) and 80 % for progesterone receptor ( PR ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Epidermal growth factor receptor ( EGFR ) is a receptor tyrosine kinase that is involved with mitogenesis and is expressed in ER negative DCIS . ^^^ We hypothesized that EGFR is central in driving proliferation in ER negative / EGFR positive DCIS . ^^^ EGFR TK inhibition with ZD 1839 offers a novel approach to the treatment of EGFR positive DCIS , regardless of ER status , and provides a potential new chemopreventative approach in patients at high risk of breast cancer . . ^^^ Epidermal growth factor receptor ( EGFR ) is a receptor tyrosine kinase that is involved with mitogenesis and is expressed in ER negative DCIS . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In this first ever study , we investigated the role of nine prognostic markers ' expression ( estrogen receptor [ ER ] , progesterone receptor [ PR ] , p 53 , C erbB 2 , epidermal growth factor receptor [ EGFR ] , cathepsin D [ CD ] , proliferating cell nuclear antigen [ PCNA ] , DNA ploidy , and S phase fraction [ SPF ] ) and disease outcome in IBC cases compared with the control group . ^^^ The expression of nine prognostic markers , that is , ER , PR , p 53 , C erbB 2 , EGFR , CD , PCNA , SPF , and DNA ploidy , was studied by immunohistochemistry and flow cytometry . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR was positive in 277 ( 26 . 9 % ) of 1029 cases which inversely correlated with the estrogen receptor ( ER ) status . ^^^ When all patients were stratified for EGFR and ER , a multivariate analysis indicated that the combination of EGFR ( + ) / ER ( ) was an independently significant factor for both DFS and OS in node negative as well as node positive breast cancer . ^^^ On the other hand , the prognostic value of combination of EGFR and ER was sufficient to achieve statistical significance based on a multivariate analysis for both DFS and OS in the subgroups of node negative as well as node positive breast cancer patients . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
To study further this process of ligand independent receptor activation , COS 7 cells without ER were transfected with both ER and epidermal growth factor receptor ( EGFR ) . ^^^ Similarly , in MCF 7 breast cancer cells that have natural expression of ER and EGFR , EGF promoted acute phosphorylation of serine and tyrosine residues in ER , and a direct interaction between ER and EGFR after treatment with EGF was found . ^^^ In confirmation of a direct interaction between ER and EGFR , activation of affinity purified EGFR tyrosine kinase in vitro stimulated the phosphorylation of recombinant ER . ^^^ The cross communication between EGFR and ER appears to promote significant stimulation of cell proliferation and a reduction in the apoptotic loss of those cells that express both receptor signaling pathways . ^^^ However , COS 7 cells transfected with both ER and EGFR show minimal stimulation of classical estrogen response element ( ERE ) dependent transcriptional activity after stimulation by EGF ligand . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We have shown that estrogen transactivates the epidermal growth factor receptor ( EGFR ) to MAP K signaling axis via the G protein coupled receptor ( GPCR ) , GPR 30 , through the release of surface bound proHB EGF from estrogen receptor ( ER ) negative human breast cancer cells [ Molecular Endocrinology 14 ( 2000 ) 1649 ] . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
RESULTS : Commonalities noted among the 3 cell lines include absent ( 0 ) chromogenic signals for ER and PR , relatively high Ki 67 proliferation indices ( 54 , 40 , and 61 % , respectively ) , and positive signals ( 1 to 3+ ) for IL 6 , IL 11 , TGF alpha , EGFR , TGF beta 1 ( LAP ) , TGF betaRII , FT , p21ras , and p 53 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The ER cross talks with a number of mitogenic signaling pathways and second messengers , like the epidermal growth factor receptor , the insulin like growth factor 1 receptor , mitogen activated protein ( MAP ) kinase , phosphatidylinositol 3 kinase / Akt , dopamine , and cyclic AMP . ^^^ We have examined whether signaling by HER2 / neu ( erbB 2 ) receptor tyrosine kinase , which can induce antiestrogen resistance , can also disrupt the tamoxifen induced interaction of ER with transcriptional corepressors . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The epidermal growth factor receptor ( EGFR ) exhibits an inverse correlation with estrogen receptor ( ER ) expression in the majority of breast cancers , predicting a poor response to endocrine therapy and poor survival rate . ^^^ We have identified a region within the first intron of the EGFR gene that mediates transcriptional repression of EGFR gene expression in ER + / low EGFR expressing but not in ER / high EGFR expressing breast cancer cells . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
AIM : Several studies have investigated the expression of the cytokeratins ( CKs ) , vimentin , the epithelial growth factor receptor ( EGFR ) , the oestrogen receptor ( ER ) , and the progesterone receptor ( PgR ) , in breast cancer , but no study has directly compared p 53 mutations with these phenotypic and differentiation markers in the same case . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Estrogen receptor ( ER ) , progesterone receptor , epidermal growth factor receptor , erbB 2 , and HSP 27 were measured by immunohistochemistry in paraffin fixed material obtained from the primary tumors at initial surgery . ^^^ Using CA 125 criteria , comparison of the CA 125 stable / responding disease with progressive disease indicated that tumors with higher ER ( P = 0 . 013 ) , lower erbB 2 ( P = 0 . 026 ) , and higher epidermal growth factor receptor ( P = 0 . 009 ) were associated with CA 125 stable / responsive disease . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Candidate prognostic biomarkers for breast cancer include elevated proliferation indices such as Ki 67 and proliferating cell nuclear antigen ( PCNA ) ; ER and PR overexpression ; markers of oncogene overexpression such as c erbB 2 , TGF a and EGFr ; indicators of apoptotic imbalance including overexpression of bcl 2 and an increased bax / bcl 2 ratio ; markers of disordered cell signaling such as p 53 nuclear protein accumulation ; alteration of differentiation signals such as overexpression of c myc and related proteins ; loss of differentiation markers such as TGF b 2 receptor and retinoic acid receptor ; and alteration of angiogenesis proteins such as VEGF overexpression . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
METHODS : Samples for the analysis of DNA ploidy , estrogen receptor ( ER ) , progesterone receptor ( PgR ) , p 53 protein and epidermal growth factor receptor ( EGFR ) were prepared from the same frozen specimens of 498 primary breast cancers . ^^^ RESULTS : Aneuploidy was significantly correlated with the absence of ER , the absence of PgR , the positivity of p 53 protein and the positivity of EGFR , and it was also significantly correlated with the combination of the absence of ER and PgR and the combination of the positivity of p 53 protein and EGFR . ^^^ A deviation in the four parameters was defined as the absence of ER , the absence of PgR , the positivity of p 53 protein or the positivity of EGFR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The prognostic biomarkers chosen for comparison represented surrogate measures of tumor : ( 1 ) . proliferation , growth and genetic instability ( mitotic and apoptotic indices , Ki 67 / MIB 1 positivity , nuclear grade , p 53 positivity ) , ( 2 ) . endocrine dependence ( estrogen receptor ( ER ) , progesterone receptors ( PR ) , pS 2 , Bcl 2 ) , ( 3 ) . growth factor receptor dependence ( ErbB 2 , EGFR / ErbB1 ) , and ( 4 ) . angiogenic , invasive and proteolytic potential ( uPA , PAI 1 , Cathepsin D , VEGF ) . ^^^ In particular , breast tumors arising in older patients have slower growth rates , are more likely to be ER positive , and are less likely to be p 53 positive , EGFR positive or ErbB 2 positive . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Many genes have been selected as targets for antisense therapy , including HER 2 / neu , PKA , TGF alpha , EGFR , TGF beta , IGFIR , P 12 , MDM 2 , BRCA , Bcl 2 , ER , VEGF , MDR , ferritin , transferrin receptor , IRE , C fos , HSP 27 , C myc , C raf and metallothionein genes . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
E 2 stimulated ERK required ER in breast cancer and endothelial cells and was substantially prevented by expression of a dominant negative EGFR or by tyrphostin AG 1478 , a specific inhibitor for EGFR tyrosine kinase activity . ^^^ The E domain is sufficient to enact these events , defining additional details of the important cross talk between membrane ER and EGFR in breast cancer . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
RESULTS : EGFR expression was positive in 87 of 241 ( 36 % ) patients with recurrent breast cancer , whereas the EGFR expression inversely correlated with the estrogen receptor ( ER ) status . ^^^ The patients with positive EGFR expression had a significantly worse postrelapse survival than those with a negative EGFR expression , whereas EGFR expression also had a postrelapse prognostic significance in patients with a positive ER status . ^^^ A multivariate analysis indicated EGFR expression to be an independently significant factor for postrelapse survival , whereas a multivariate analysis in which the ER status was added to variables indicated that ER status but not EGFR expression to be an independently significant factor . ^^^ CONCLUSIONS : EGFR expression had prognostic significance in recurrent breast cancer , whereas its prognostic value was not independent of the ER status . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Estrogen receptors ( ER ) , progesterone receptors ( PR ) , pS 2 and cathepsin D cytosolic levels were determined , as well as levels of epidermal growth factor receptor ( EGFR ) in cell surfaces . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
It has been reported that overexpression of the epidermal growth factor receptor ( erbB 1 ) or its homologous receptor , HER 2 ( erbB 2 ) , can confer antiestrogen resistance to estrogen receptor ( ER ) positive human breast cancer cells . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Interactions between the estrogen receptor ( ER ) and the epidermal growth factor receptor ( EGFR ) contribute to the biological effects of these binding protein families . ^^^ EGFR stimulates DNA synthesis and gene transcription in the uterus , related in part to estrogen independent activation of the nuclear ER . ^^^ Overall , the bidirectional cross talk between EGFR and cellular pools of ER contributes to reproductive organ physiology and pathophysiology . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
To evaluate whether the combination of biological parameters increases their prognostic value , the expression of epidermal growth factor receptor ( EGFR ) , DNA ploidy , and estrogen receptor ( ER ) status were analyzed on 998 patients with breast cancer . ^^^ Poor findings for each biological parameter were positive for EGFR , aneuploid for DNA ploidy , and negative for ER . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
This review describes the roles of HER 2 , epidermal growth factor receptor ( EGFR ) , oestrogen receptor ( ER ) / progesterone receptor ( PgR ) , Ki 67 , Bcl 2 , p 53 and gene expression profiling in predicting responses to endocrine , cytotoxic and biological therapies . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
ER , PR , p 53 , C erbB 2 , EGFR , Cathepsin D , PCNA , DNA ploidy and S phase fraction , were studied by immunohistochemistry and flow cytometry . ^^^ ER / PR , EGFR , PCNA and metastatic potential , there was no significant difference in the OS of PAC vs . control group if compared stage for stage . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : We analyzed 1 , 025 invasive ductal carcinomas , consisting of 424 papillotubular , 330 solid tubular and 271 scirrhous cases , with regard to the status of estrogen receptor ( ER ) , progesterone receptor ( PgR ) , DNA ploidy , epidermal growth factor receptor ( EGFR ) and p 53 protein . ^^^ RESULTS : Absence of ER and PgR , aneuploidy , EGFR positivity and p 53 protein positivity were all observed significantly more frequently in solid tubular tumors than in the other two types . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
BACKGROUND : We recently confirmed , in a series of 365 human breast cancers , that EGFR and c erbB 2 were associated with estradiol receptors ( ER ) and / or progesterone receptors ( PgR ) negative tumors . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
OBJECTIVE : To study the clinicopathological features , the histogenesis , and the correlation between the expressions of estrogen receptor ( ER ) and epidermal growth factor receptor ( EGFR ) in leiomyoma of the vagina . ^^^ METHODS : The data of 26 patients with leiomyoma of the vagina were analyzed retrospectively , and S P immunohistochemistry was used to detect smooth muscle actin ( SMA ) , S 100 protein , CD 34 , ER and EGFR in the tumor tissues . ^^^ Positive expression rates for ER and EGFR were 38 . 5 ( 10 / 26 ) and 34 . 6 ( 9 / 26 ) respectively . ^^^ There was a significant positive correlation between the expressions of ER and EGFR ( Fisher exact , P = 0 . 0023 , r = 0 . 75 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The findings on the well known parameters estrogen receptor ( ER ) alpha , progesterone receptor ( PR ) and epidermal growth factor receptor ( EGFR ) are in good agreement with previous reports , thus documenting the usefulness of the real time RT PCR technique for multiparametric RNA analysis . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Oestrogen receptor ( ER ) levels are usually maintained on acquisition of tamoxifen resistance in the clinic , however , tumour re growth is associated with increased expression of epidermal growth factor receptor ( EGFR ) and activation of the mitogen activated protein kinase ( MAPK ) pathway . ^^^ These results suggest that the ER modulates EGFR / MAPK signalling efficiency in TAM R cells possibly through the regulation of TGFalpha availability . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
These paired specimens were simultaneously analyzed for Ki 67 , ER , progesterone receptor ( PgR ) , trefoil factor 1 ( PS 2 ) , HER 1 ( epidermal growth factor receptor ) , and HER 2 ( ErbB 2 or neu ) by semiquantitative immunohistochemistry . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The significantly higher concentrations of EGFR were detected in patients under 60 years old ( p = 0 . 042 ) , undifferentiated tumors ( p = 0 . 04 ) , and carcinomas with negative ER and PR ( p < 0 . 019 y p < 0018 , respectively ) . ^^^ In addition , there was a negative correlation between EGFR and the ER and PR levels ( p < 0 . 05 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
To investigate the significance of these mechanisms on an early stage of human breast tumour growth , we studied the expression of EGFR ( ErbB 1 ) , HER 2 / neu ( ErbB 2 ) , cyclin D 1 , p 21 and p 53 as well as oestrogen ( ER ) and progesterone receptor ( PgR ) in paraffin sections of 45 ductal carcinoma in situ ( DCIS ) by immunohistochemistry . ^^^ Besides , ER mediated upregulation of cyclin D 1 seems to be a possible mechanism of maintaining cell proliferation in DCIS in case of EGFR and HER 2 / neu negativity . . ^^^ ErbB negative tumours with increased proliferative activity were ER and cyclin D 1 positive . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Through the analysis of the transient expression of the luciferase reporter gene in HeLa cells , an evaluation has been made of the transcriptional activity of oestrogens and of selective oestrogen receptor ( ER ) modulators ( SERMs ) , mediated by the alpha and beta isoforms of the ER , on the epidermal growth factor receptor gene promoter . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
To test the hypothesis that inhibition of the EGFR signalling pathway affects the antitumour effect of endocrine therapy , an EGFR tyrosine kinase inhibitor ( EGFR TKI ) , gefitinib , and an oestrogen receptor ( ER ) antagonist , fulvestrant , were administered to human breast cancer cells . ^^^ These results suggest an additive interaction between the EGFR TKI gefitinib and the antioestrogen fulvestrant in ER positive breast cancer cells . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
CD 46 exhibited the most striking pattern of association , with increased levels of expression being associated with ER positive samples and lower levels of expression associated with a loss of differentiation and epidermal growth factor receptor positivity . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We determined whether this diet induced mammary gland differentiation is mediated through the estrogenic pathway via epidermal growth factor receptor ( EGFR ) and estrogen receptor ( ER ) signaling . ^^^ At postnatal day ( PND ) 21 and the proestrus phase on PND 49 51 , mammary glands of offspring were analyzed for morphology , cell proliferation , and expression of EGFR , epidermal growth factor ( EGF ) , transforming growth factor alpha , ER alpha , and ER beta . ^^^ Epithelial EGFR and stromal fibroblast EGF were increased by SDG , whereas epithelial ER beta was decreased by FS . ^^^ In conclusion , exposure to FS or SDG during suckling enhanced mammary gland morphogenesis by modulation of EGFR and ER signaling , which led to more differentiated mammary glands at PND 49 51 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Instead , it did not affect the exocytic trafficking of a GtsO 45 derived chimeric protein bearing 30 juxtamembrane residues from the cytosolic domain of the epidermal growth factor receptor that contains a variant ER exit motif ( ERE ) and an unconventional proline based basolateral sorting signal . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Immunohistochemical double staining with estrogen receptor ( ER ) and epidermal growth factor receptor 2 ( HER 2 ) was conducted in tissue sample of 125 women with invasive breast cancer . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Because expression of EGFR is inversely related to expression of the estrogen receptor ( ER ) and is associated with resistance to currently available breast cancer therapies , EGFR targeted therapies may be valuable in the treatment of ER negative tumors and endocrine resistant , ER positive tumors . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Findings were related to tumour size , type , grade , node status , oestrogen ( ER ) , progesterone , and epidermal growth factor receptor ( EGFR ) expression for invasive carcinomas and to grade and receptors for in situ carcinomas . ^^^ For invasive carcinomas , the presence of P cadherin significantly correlated with high grade , lack of ER and presence of EGFR , but not tumour size or node status . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Tumor grade was discordant in 36 % of patients Discordance for estrogen receptor ( ER ) = 2 % ; MIB 1 = 18 % ; Her2 / neu = 9 % ; EGFR = 10 % ; p 53 = 9 % ; and bcl 2 = 0 % . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
To evaluate this concern , we prepared a single TMA block from 125 invasive breast carcinomas collected in a population based case control study conducted in Poland and compared estrogen receptor ( ER alpha ) , progesterone receptor ( PR ) , and human epidermal growth factor receptor 2 ( HER 2 ) expression in sections cut and stored for 6 months at room temperature with sections cut from the same TMA block and stained on the same day . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Inhibitors were employed to determine pathway ( ER , EGFR , membrane organization , PI 3 kinase , Src kinase , Ca2+ ) involvement and timing of pathway activations ; all affected ERK activation as early as 3 6 min , suggesting simultaneous , not sequential , activation . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Of the seven cell lines obtained , three fast growing and estrogen receptor ( ER ) + / progesterone receptor ( PgR ) + / EGF receptor ( EGFR ) + have been characterized . ^^^ The mRNA expression of type alpha and beta ER as well as EGFR , was confirmed by RT PCR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
RESULTS : Expression of FOXP 1 was significantly positively associated with ER ( P = 0 . 03 ) and negatively with epidermal growth factor receptor ( P = 0 . 01 ) but no association with age ( P = 0 . 91 ) , lymph node status ( P = 0 . 94 ) , size ( P = 0 . 76 ) , or grade ( P = 0 . 22 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We examined 24 peritoneal and nine pleural malignant mesotheliomas with a battery of immunohistochemical markers ( cytokeratin AE1 / 3 , calretinin , c kit / CD117 , desmin , epidermal growth factor receptor ( EGFR ) , estrogen receptors ( ER ) , progesterone receptors ( PR ) , MIB 1 , and cleaved caspase 3 ) in an attempt to distinguish any differences in this tumor arising in these two distinct locations . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Tissue microarrays ( TMAs ) were used to determine the immunohistochemical profile of eight proteins including E cadherin , EGFR , oestrogen and progesterone receptor ( ER and PR ) , MIB 1 , ERBB 2 , MUC 1 , and P 53 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
This investigation is beginning to elucidate the influence of growth factor pathways that interact with the ER , especially HER 1 ( epidermal growth factor receptor [ EGFR ] ) and HER 2 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Current insights into the molecular basis for these resistance patterns are rudimentary , but are most clearly illuminated by investigations that focus on the crosstalk between the ErbB or HER peptide growth factor family and the ER . ^^^ Efficacy was variable , however , despite the selection of patients on the basis of ER and ErbB 2 coexpression . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
ER hormone responsiveness is widely believed to be influenced by enhanced cross talk of ER with overexpressed human epidermal growth factor receptor 2 ( HER 2 ) , and a subgroup of ER positive tumors coexpress high HER 2 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Akt activation was blocked by wortmannin and LY 294 , 002 , two inhibitors of PI 3 K ; by genistein , a protein tyrosine kinase inhibitor and an ER agonist ; by AG 825 , a selective ErbB 2 inhibitor ; and by the antiestrogens ICI 182 , 780 and 4 hydroxy tamoxifen ; but not by rapamycin , an inhibitor of the ribosomal protein kinase p70S6K ; nor by AG 30 , a selective epidermal growth factor receptor inhibitor . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Interestingly , high ER and PR expression was seen in active and inactive endometria , but only the former were EGFR positive and had high proliferative ( Ki 67 ) and angiogenic activity . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We investigated the immunoreactivity of sstr2A in 34 cases of human breast carcinoma and correlated these findings with the immunoreactivity of the estrogen receptor ( ER ) , epidermal growth factor receptor ( EGFR ) , transforming growth factor alpha ( TGFalpha ) and insulin like growth factor 1 ( IGF 1 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
It also suggests that , in this group of breast cancers , the most common upstream regulator of AKT may be ER rather than PTEN , EGFR or Her2 . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
ER negative tumors show a higher expression of p 53 , CerbB 2 and epidermal growth factor receptor compared to ER positive breast cancer . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
METHODS : EGF receptor ( EGFR ) was detected by flow cytometry and estrogen receptor ( ER ) by Northern blot . ^^^ CONCLUSION : Cultured epithelial ovarian carcinoma cell lines express EGFR ( 5 / 5 ) and can express ER ( 1 / 5 ) . ^^^ Unexpectedly , the antineoplastic agent suramin increased growth of ER positive ovarian carcinoma cells in an EGFR dependent manner . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
CONCLUSION : Patients with HER 2 amplification and HER 1 expression had lower ER levels and were modestly less responsive to tamoxifen , suggesting that molecular events in addition to those involving the ErbB receptors are important in determining the endocrine resistant phenotype . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
For indeed most , if not all , postmenopausal atrophic endometria harboring adenocarcinomas contain actively proliferating glands , with high Ki 67 proliferation index , high epidermal growth factor receptor ( EGFR ) activity , high microvessel density ( MVD ) , and rich in estrogen and progesterone receptors ( ER and PR ) , indicative of a continuous low level estrogenic stimulation . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Breast cancer tissues from 271 cases were analyzed immunologically for c erbB 2 oncoprotein ( HER 2 / neu ) , epidermal growth factor receptor ( EGF R ) and estrogen receptor ( ER ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Therefore , ER and PgR expression alone are unlikely to determine a patient ' s optimal treatment regimen , particularly when the amount of ' cross talk ' between different pathways , such as the epidermal growth factor receptor pathway , is considered . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We have targeted both the estrogen receptor ( ER ) and the epidermal growth factor receptor ( EGFR ) pathways using an ER antagonist , fulvestrant ( `` Faslodex ' ' ) , and the selective EGFR tyrosine kinase inhibitor , gefitinib ( `` Iressa ' ' ) , in non small cell lung cancer ( NSCLC ) cells . ^^^ Rapid activation of phospho EGFR and phospho p44 / p42 mitogen activated protein kinase by estrogen was observed , indicating nonnuclear ER transactivation of EGFR . ^^^ The relative responses to gefitinib and fulvestrant were similar regardless of ER and EGFR expression and mutation status . ^^^ These studies provide evidence of a functional interaction between the ER and the EGFR pathways in NSCLC . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In the following studies , the effects of a novel conditionally activated form of EGF R , 5 ErbB : ER , on the morphological transformation of NIH 3T3 cells and the abrogation of hematopoietic cell cytokine dependence were investigated . ^^^ The EGF R inhibitor AG 1478 effectively inhibited MEK , ERK and Akt activation , and induced apoptosis when the cells were grown in response to 5 ErbB : ER . ^^^ This novel 5 ErbB : ER construct and these conditionally transformed cell lines will be useful to further elucidate ErbB mediated signal transduction and to determine the effectiveness of various inhibitors in targeting different aspects of EGF R mediated signal transduction and malignant transformation . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
METHODS : Immunohistochemical staining was undertaken for estrogen ( ER ) and progesterone ( PR ) receptors , epidermal growth factor receptor 2 ( Her 2 ) , p 53 , vascular endothelial factor ( VEGF ) , and hypoxia inducible factor 1alpha ( HIF 1alpha ) and the levels of these markers was compared to e cadherin expression in a high risk African American patient population . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
A cell based RNAi screen for loss of function phenotypes that alleviate ER accumulation of cSpi identified several genes , including the small wing ( sl ) gene encoding a PLCgamma . sl mutants compromised ER accumulation of cSpi in embryos , yet they exhibit EGFR hyperactivation phenotypes predominantly in the eye . ^^^ Retention of cSpi in the ER provides a novel mechanism for restricting active ligand levels and hence the range of EGFR activation in the developing eye . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
METHODS : The retrovirus pBabe 5R , which encodes an erbB 2 single chain antibody with an endoplasmic reticulum ( ER ) targeting sequence , and control pBabe puro were used to infect THCE cells ( an SV 40 immortalized HCEC line ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The multiple molecular cross talks , including the interactions between phosphatidylinositol 3 kinase / Akt and mitogen activated protein kinase signaling pathways and two diverse receptors ( i . e . , ER alpha and EGFR ) , were essential in the event of EGF induced WISP 2 / CCN5 up regulation in MCF 7 cells . ^^^ Moreover , EGF action on WISP 2 / CCN5 is restricted to ER and EGFR positive noninvasive breast tumor cells , and this effect of EGF can not be instigated in ER alpha negative and EGFR positive normal or invasive breast tumor cells by introducing ER alpha . ^^^ Finally , regulation of phosphorylation of ER alpha and EGFR may play critical roles in EGF induced transcriptional activation of WISP 2 gene in breast tumor cells . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The lesion was resected , and the tissue was subjected to immunostaining for gene products associated with pregnancy , including estrogen receptor ( ER ) , progesterone receptor ( PR ) , platelet derived growth factor receptor B ( PDGFRB ) , fibroblastic growth factor receptor 2 ( FGFR 2 ) , epidermal growth factor receptor ( EGFR ) and human placental lactogen ( hPL ) . ^^^ No specific staining for ER , EGFR , or hPL was identified . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
DESIGN : Mucosal biopsy specimens from 47 adenocarcinomas involving the gastrointestinal tract ( 28 primary gastric carcinomas and 19 metastatic breast carcinomas ) and 16 control cases of primary breast carcinomas without metastasis were immunohistochemically stained for estrogen receptor protein ( ER ) , progesterone receptor protein ( PR ) , gross cystic disease fluid protein ( GCDFP ) , human epidermal growth factor receptor 2 protein , cytokeratin ( CK ) 5 / 6 , CK / 7 , CK / 20 , a panel of mucin glycoprotein antigens ( MUC 2 , MUC 3 , MUC5AC , and MUC 6 ) , monoclonal antibody DAS 1 , and caudal type homeobox transcription factor CDX 2 and compared between primary and metastatic adenocarcinomas . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The influence of ER , PR , and EGFR on patient survival was analyzed in two ways : 1 ) as a separate parameter of each receptor and 2 ) as a common parameter consisting of 8 variables of concomitant presence or absence of the receptors . ^^^ In the second analysis it was found that patients with receptor status ER+PR+EGFR+ ; ER PR+EGFR ; ER+PR+EGFR ; and ER PR EGFR were having better parameters of DFS and OS ( relative risks for DFS or OS were between 0 . 22 1 . 16 ) . ^^^ The patients with receptor status : ER PR+EGFR+ ; ER+PR EGFR , ER PR EGFR+ and ER+PR EGFR+ exhibited a more aggressive disease course ( relative risks for DFS and OS were between 1 . 46 3 . 95 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
However , most important biomarkers in a clinical setting are ER ( estrogen receptors ) and HER 2 ( human epidermal growth factor receptor 2 ) , but still only as predictive markers for tamoxifen and trastuzumab therapy . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Tissue sections from formalin fixed paraffin embedded blocks were stained for the presence of estrogen receptor ( ER ) , progesterone receptor ( PgR ) , HER ( human epidermal growth factor receptor ) 2 , p 53 protein , Bcl 2 , and MIB 1 ( Ki 67 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
However , tumors typically depend on only one of these pathways and may overexpress either estrogen receptor ( ER ) or EGF receptor ( EGFR ) and related family members . ^^^ Tumors overexpressing EGFR are more aggressive than those expressing ER . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Prolonged endocrine therapy can be associated with an acquired increase in peptide growth factor signaling ( EGFR , HER 2 ) , together with cross talk activation of ER dependent gene transcription and cell growth that leads to endocrine resistance . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Interestingly , EGFR and its activated forms were all significantly inversely correlated with PgR expression ( p=0 . 011 , p=0 . 033 and p=0 . 032 , respectively ) , and ptyr 845 EGFR was also inversely correlated with ER expression ( p=0 . 008 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Treatment of 5 ErbB : ER cells with the EGFR inhibitor AG 1478 efficiently induced apoptosis . ^^^ Induction of apoptosis and prevention of cell cycle progression by the EGFR inhibitor were only observed when the cells were grown in response to 5 ErbB : ER activation demonstrating specificity . ^^^ To circumvent these problems , we subcloned 5 ErbB into a vector which contains the estrogen receptor hormone binding domain ( ER ) which renders the 5 ErbB : ER protein dependent upon beta estradiol for activity . 5 ErbB : ER conditionally abrogated the cytokine dependence of hematopoietic cells more efficiently than activated 5 Ha Ras , 5 Src , Raf or Akt . ^^^ Abrogation of cytokine dependence by 5 ErbB : ER was not due to the synthesis of autocrine growth factors . ^^^ In contrast , the other inhibitors suppressed cell cycle progression when the cells were grown in response to 5 ErbB : ER or the cytokine interleukin 3 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
BACKGROUND : Some oestrogen receptor ( ER ) positive breast cancers express epidermal growth factor receptor ( EGFR ) , but whether inhibition of EGFR can suppress proliferation of breast cancer cells and ER function is not known . ^^^ METHODS : In a double blind , placebo controlled randomised trial of 56 postmenopausal patients with ER positive and EGFR positive primary breast cancer , 27 women were randomly assigned to the tyrosine kinase inhibitor of EGFR gefitinib ( 250 mg given orally once a day ) and the aromatase inhibitor anastrozole ( 1 mg given orally once a day ) , and 29 women to gefitinib ( 250 mg given orally once a day ) and placebo of identical appearance to anastrozole given orally once a day , all given for 4 6 weeks before surgery . ^^^ Secondary outcomes were reduction in EGFR phosphorylation at Tyr 845 , reduction in ER phosphorylation at Ser 118 , tumour size , and toxic effects . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In the human lens , stimulation of tyrosine kinase coupled growth factor receptors such as epidermal growth factor receptor ( EGFR ) can induce calcium release from endoplasmic reticulum ( ER ) stores . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Breast cancer models of acquired tamoxifen resistance , oestrogen receptor ( ER ) + / ER de novo resistance and gene transfer studies cumulatively demonstrate the increased importance of growth factor receptor signalling , notably the epidermal growth factor receptor ( EGFR ) / HER2 , in tamoxifen resistance . ^^^ Our recent in vitro studies also suggest that EGFR signalling productively cross talks with insulin like growth factor receptor ( IGF 1R ) and , where present , activates ER on key AF 1 serine residues to facilitate acquired tamoxifen resistant growth . ^^^ We also present our clinical immunohistochemical findings that IGF 1R expression , its phosphorylation on tyrosine 1316 , and also phosphorylation on serine 118 of ER are not only prominent in ER+ tamoxifen responsive disease , but are also detectable in ER+ de novo and acquired tamoxifen resistant breast cancer , where there is evidence of EGFR / ER cross talk . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
To date , the only widely accepted markers for routine use in breast cancer are the oestrogen receptor ( ER ) , progesterone receptor ( PR ) and human epidermal growth factor receptor , HER 2 ( c erbB2 / neu ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Increased benefit was found in patients with tumours expressing both ER and progesterone receptor ( PgR ) compared with other combinations , although good activity was reported in patients expressing either ER or PgR as well as in tumours expressing human epidermal growth factor receptor 2 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The known cancer associated proteins ( ER , EGFR , STATS , tissue factor and Bcl xL ) were selected for study . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The purpose of present study is to examine ER ( ) / HER2 ( ) breast cancers , with a particular focus on epidermal growth factor receptor ( EGFR ) . ^^^ EGFR , p 53 and vimentin were highly expressed in ER ( ) / HER2 ( ) breast cancer cells ( p < 0 . 01 ) . ^^^ Overall , the ER ( ) / HER2 ( ) breast cancer showed the highest Ki 67 LI , the most frequent expression of EGFR , p 53 and vimentin , as well as the lowest expression of cyclinD 1 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We studied the immunohistochemical expression of HER 2 / neu , epidermal growth factor receptor ( EGFR ) , vascular endothelial growth factor ( VEGF ) , cyclooxygenase 2 ( COX 2 ) , estrogen receptor ( ER ) , and progesterone receptor ( PR ) in uterine cervical small cell and large cell neuroendocrine carcinomas ( SCNECs and LCNECs ) from 24 patients seen at The University of Texas M . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
This includes results from our clinical breast cancer trial 1839IL / 0057 that demonstrate the efficacy of gefitinib within ER positive , tamoxifen resistant patients with locally advanced / metastatic disease , where parallel decreases in EGFR signal transduction and the Ki 67 ( MIB 1 ) proliferation marker can be detected as predicted from model system studies . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Cross talk between the ER and various growth factor receptor signaling pathways , including human epidermal growth factor receptor 2 , and the insulin like growth factor pathway , may also be implicated . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Here , we show that , through EGF receptor ( EGFR ) and erb B 2 , EGF induces tyrosine phosphorylation of ER preassociated with AR , thereby triggering the assembly of ER / AR with Src and EGFR . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
New sections , with core and surgical specimens on the same slides , were stained for estrogen receptor ( ER ) , progesterone receptor ( PR ) , and human epidermal growth factor receptor 2 ( HER 2 ) immunohistochemistry ( IHC ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
We have analyzed a breast cancer TMA containing 2 , 517 breast tissues , including 2 , 222 neoplastic and 295 normal or premalignant samples , for Ki 67 labeling index ( Ki 67 LI ) and additional markers with a known relationship to Ki 67 LI by immunohistochemistry ( ER , PR , Bcl 2 , Egfr , p 16 , p 53 ) and Fluorescence in situ hybridization ( HER 2 , MDM 2 , CCND 1 , MYC ) . ^^^ A high Ki 67 LI was linked to tumor phenotype including grade ( p < 0 . 0001 ) , stage ( p < 0 . 0001 ) , nodal stage ( p = 0 . 0018 ) , and patient prognosis ( p < 0 . 0001 ) , elevated protein levels of p 53 , p 16 and Egfr , reduced levels of Bcl 2 , ER , and PR ( p < 0 . 0001 each ) , as well as amplifications of HER 2 , MYC , CCND 1 and MDM 2 ( p < 0 . 0001 each ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : To investigate this , pre treatment biopsies from 517 patients with locally advanced breast cancer were analyzed for expression of estrogen receptor [ ER ] , progesterone receptor [ PgR ] , Her 2 / neu , epidermal growth factor receptor [ EGF R ] , p 53 , Bcl 2 and MIB 1 by immunohistochemistry [ IHC ] , and these data were compared to the pathological response after preoperative epirubicine / cyclophosphamide [ EC ] chemotherapy ( + / radiotherapy ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Immunohistochemistry for estrogen receptor ( ER ) , HER 2 , EGFR , smooth muscle actin ( SMA ) , p 63 , CD 10 , cytokeratin 5 / 6 , cytokeratin 8 / 18 , and vimentin was performed on 18 basal like , 16 luminal , and 12 HER2+ tumors . ^^^ The most consistent immunophenotype seen in the basal like tumors was negativity for ER and HER 2 , and positivity for vimentin , EGFR , cytokeratin 8 / 18 , and cytokeratin 5 / 6 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Phosphorylated ERBB 2 correlated with EGFR and ERBB 2 , and inversely with oestrogen receptor ( ER ) , progesterone receptor ( PgR ) and ERBB 4 expression levels . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In this report we now show that overexpression of EGFR or activated AKT 2 in MCF 7 cells leads to phosphorylation of Ser 167 in the AF 1 domain of ERalpha , enhanced ER amplified in breast cancer 1 ( ER : AIB 1 ) interaction in the presence of tamoxifen , and resistance to tamoxifen . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Furthermore , proline , glutamic acid , leucine rich protein 1 , an ER coactivator involved in both genomic and nongenomic signaling pathways , is activated by epidermal growth factor receptor and plays a prominent role in resistance to tamoxifen . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Flow cytometric analysis was used for the detection of ER , PR , HER 2 / neu , epidermal growth factor receptor ( EGFR ) , and E cadherin . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Immunohistochemical staining for ER , progesterone receptor ( PgR ) , HER 2 and epidermal growth factor receptor ( EGFR ) was undertaken . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
These lesions are estrogen receptor ( ER ) negative , progesterone receptor ( PR ) negative , and HER 2 negative ( triple negative ) , and typically express basal cytokeratins , epidermal growth factor receptor ( EGFR ) , and / or c kit . ^^^ We studied 66 cases of high nuclear grade DCIS using antibodies to ER , PR , HER 2 , three basal cytokeratins , EGFR , and c kit to determine the frequency of the triple negative phenotype , and to determine the relationship between the triple negative phenotype and expression of basal cytokeratins and other biomarkers characteristically expressed by invasive basal like carcinomas . ^^^ We conclude that a small proportion of high grade ductal carcinomas in situ exhibit an ER negative / PR negative / HER2 negative ( triple negative ) phenotype , and these lesions more commonly show expression of basal cytokeratins and / or EGFR than nontriple negative high grade DCIS . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
The interaction between oestrogen receptors ( ER ) in the lung and epidermal growth factor receptor ( EGFR ) suggests a potential role for endocrine manipulation in the treatment of NSCLC . ^^^ Early trials have assessed the interaction between the ER and EGFR signalling . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Many BRCA 1 related tumors have a distinct histological characteristics which together have been called `` basal like . ' ' Typically such tumors are ER , HER 2 and express cytokeratin 5 / 6 , cytokeratin 8 / 18 , EGFR and vimentin . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
PURPOSE : To investigate the impact of human epidermal growth factor receptor ( HER ) 1 and HER 2 gene amplification on endocrine therapy responsiveness , a fluorescence in situ hybridization ( FISH ) study was conducted on tumor samples from 305 postmenopausal patients with stage 2 and 3 estrogen receptor ( ER ) positive ( ER > or = 10 % ) breast cancers treated on two independent neoadjuvant endocrine therapy trials . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Positive to negative change was prominent with intervening endocrine treatment , and it was significant ( p=0 . 015 ) for ER by multiple regression analysis of age , interval of sampling and from prior surgery , intervening chemotherapy , endocrine therapy and human epidermal growth factor receptor 2 ( HER 2 ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Predictive markers of response like the estrogen receptor ( ER ) and the human epidermal growth factor receptor 2 ( HER 2 ) help identify those most likely to derive a survival benefit from adjuvant therapy with anti estrogens and trastuzumab therapy , respectively . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
RESULTS : On a univariate basis , the following features were significantly associated with worse survival : high pathologic tumor or nodal class , histologic grade , epidermal growth factor receptor , ERBB 2 , MYC , or TP 53 ; absent estrogen receptor ( ER ) or progesterone receptor ; and low BCL 2 . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
In this communication we propose that patients with estrogen receptor ( ER ) negative / progesterone receptor ( PR ) negative / HER 2 negative tumors , which currently present a therapeutic challenge for the oncologist , may be the subgroup of breast cancer patients that might benefit from specific EGFR targeted therapies . . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
EGFR overexpression is associated with reduced survival and is inversely correlated with expression of estrogen receptor ( ER ) . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
For instance , ER interacts with EGFR in a number of ways , affecting development of a number of epithelial structures . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
Six genes were over expressed ( ERBB 2 , TGFA , HB EGF , AREG , EREG and EPGN ) , 3 were under expressed ( ERBB 1 , ERBB 4 and NRG 4 ) and 2 were over or under expressed ( ERBB 3 and BTC ) . ^^^ The most marked differences in expression were ERBB 3 , EREG and NRG 4 between superficial and muscle invasive tumors ( p = 0 . 0069 , 0 . 00007 and 0 . 0000001 , respectively ) , and TGFA and NRG 4 between low and high grade superficial tumors , and between pT 1 or greater and pTa tumors . ^^^
Interacting proteins: O14944 and P00533	Pubmed	SVM Score :0.0
AIMS : Recently , an immunohistochemical panel comprising antibodies against HER 2 , oestrogen receptor ( ER ) , epidermal growth factor receptor ( EGFR ) and cytokeratin ( CK ) 5 / 6 was reported to identify basal like breast carcinomas , as defined by cDNA microarrays . ^^^ Immunohistochemistry with antibodies for HER 2 , ER , EGFR , CK5 / 6 , CK 14 and p 63 was performed according to standard methods . ^^^