Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: O14813 and P61296	Pubmed	SVM Score :0.0
The interaction between dHAND and Arix at the dopamine beta hydroxylase promoter region is independent of direct dHAND binding to DNA . ^^^ Transcription factors dHAND , a basic helix loop helix protein , and Arix / Phox2a , a homeoprotein , have been demonstrated to play a role in the differentiation and maintenance of catecholaminergic neurons . ^^^ Three Arix regulatory sites have been identified in the DBH promoter proximal region , but there is no such evidence for dHAND . ^^^ Cotransfection with a DBH promoter luciferase reporter construct plus dHAND or dHAND E 12 expression plasmids did not alter luciferase activity , whereas transfection with Arix resulted in a 2 . 5 fold stimulation of luciferase activity . ^^^ However , a 5 . 5 fold increase was observed when Arix and dHAND were combined , and an 8 fold level of expression was observed when Arix was transfected with a dHAND mutant lacking the basic DNA binding domain . ^^^
Interacting proteins: O14813 and P61296	Pubmed	SVM Score :0.0
Similar to Phox2b , cells expressing Phox2a and Hand 2 ( dHand ) clearly outnumber TH positive cells . ^^^
Interacting proteins: O14813 and P61296	Pubmed	SVM Score :0.0
The generation of noradrenergic sympathetic neurons is controlled by BMPs and the downstream transcription factors Mash 1 , Phox2b , Phox2a and dHand . ^^^
Interacting proteins: O14813 and P61296	Pubmed	SVM Score :0.0
HAND 2 synergistically enhances transcription of dopamine beta hydroxylase in the presence of Phox2a . ^^^ Noradrenergic neuronal identity and differentiation are controlled by cascades of transcription factors acting downstream of BMP 4 , including the basic helix loop helix DNA binding protein HAND 2 and the homeodomain factor Phox2a . ^^^ We have examined the interaction of HAND 2 and Phox2a at the DBH promoter . ^^^ Using transient transfection of P 19 or NT 2 cells , HAND 2 is shown to synergistically enhance Phox2a driven transcriptional activity at the DBH promoter , an effect that is enhanced by cAMP . ^^^ While mutation of the Phox2a homeodomain binding sites HD 1 , HD 2 , and HD 3 results in the loss of HAND2 / Phox2a transactivation of DBH , it is the interaction of HAND2 / Phox2a at the CRE / AP1 HD1 / 2 domains in the DBH enhancer that are required for synergistic activation by HAND 2 . ^^^
Interacting proteins: O14813 and P61296	Pubmed	SVM Score :0.0
In contrast to MASH 1 ( / ) mice they do not express dHand , Phox2A , c ret , neurofilament , neuron specific tubulin , and NCAM and appear ultrastructurally more immature . ^^^
Interacting proteins: O14813 and P61296	Pubmed	SVM Score :0.0
Compiled data from many groups suggest that neurogenesis and cell type specific noradrenergic marker gene regulation is coordinated through the expression and function of the basic helix loop helix DNA binding protein HAND 2 and the homeodomain DNA binding protein Phox2a . ^^^ The expression of transcripts encoding HAND 2 and Phox2a is regulated via canonical BMP signaling and thus affects both neurogenesis and cell type specific gene expression . ^^^ Signaling affected by cAMP is necessary for the transcription of Phox2a as well as regulation of DBH promoter transactivation by Phox2a and HAND 2 . ^^^
Interacting proteins: O14813 and P61296	Pubmed	SVM Score :0.0
The noradrenergic cell type is characterized by the expression of proteins involved in the biosynthesis , transport , and secretion of noradrenaline and is dependent on the sequential and combinatorial expression of numerous transcription factors , including Phox2a , Phox2b , dHAND , GATA 2 , GATA 3 , and MASH 1 . ^^^ Phox2a and Phox2b transactivate the promoter of the gene encoding the noradrenergic biosynthetic enzyme , dopamine beta hydroxylase ( DBH ) , and dHAND potentiates the activity of Phox2a . ^^^ The interaction between Phox2a and dHAND is analyzed by fluorescent anisotropy , which demonstrates that dHAND causes an eightfold increase in the affinity of Phox2a for its recognition sites on the DBH promoter region . ^^^ Together with Phox2a and Phox2b , dHAND is bound to the PHOX2B promoter and is also associated with the GATA 2 and eHAND genes in the absence of the Phox 2 proteins . ^^^ The Phox 2 proteins were found to share all target genes , whereas dHAND binds to genes independently of Phox2a . . ^^^
Interacting proteins: O14813 and P61296	Pubmed	SVM Score :0.0
Sympathetic neurons are specified during their development from neural crest precursors by a network of crossregulatory transcription factors , which includes Mash 1 , Phox2b , Hand 2 and Phox2a . ^^^ Gata 2 expression starts after Mash 1 , Phox2b , Hand 2 and Phox2a expression , but before the onset of the noradrenergic marker genes Th and Dbh , and is maintained throughout development . ^^^ Gata 2 expression is affected in the chick embryo by Bmp gain and loss of function experiments , and by overexpression of Phox2b , Phox2a , Hand 2 and Mash 1 . ^^^