| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Death receptor 5 , a new member of the TNFR family , and DR 4 induce FADD dependent apoptosis and activate the NF kappaB pathway . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| This activation is regulated by the recruitment of an adaptor protein Fas associating death domain ( FADD ) to TRAIL death receptors , death receptor 4 ( DR 4 , TRAIL R 1 ) and death receptor 5 ( DR 5 TRAIL R 2 ) . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Fas associated protein with death domain ( FADD ) independent recruitment of c FLIPL to death receptor 5 . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Whereas both S and N type cell lines express TRAIL R 2 , FADD , and caspase 3 and 10 , only S type cells express caspase 8 . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Although both S and N type cell lines express TRAIL R 2 , FADD , and caspases 3 and 10 , only S type cells express caspase 8 . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) induced apoptosis is mediated by its receptors DR 4 ( TRAIL R 1 ) and DR 5 ( TRAIL R 2 ) and the adapter protein Fas associated death domain protein ( FADD ) . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| CD 95 , TNF related apoptosis inducing ligand ( TRAIL R 1 ) , and TRAIL R 2 bind FADD directly , whereas recruitment to TNF R 1 is indirect through another adaptor TNF receptor associated death domain protein ( TRADD ) . ^^^ Under the same conditions , the intracellular domain of both CD 95 and TRAIL R 2 bound both FADD and caspase 8 . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| In an in vitro binding assay , the intracellular domains of both TRAIL R 1 and TRAIL R 2 bound FADD : activation of PKC significantly inhibited this interaction suggesting that PKC may be targeting key apical components of death receptor signaling . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| TRAIL receptor 2 signals apoptosis through FADD and caspase 8 . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| In particular , the expression of procaspase 8 gene and subsequent cleavage of caspase 8 were markedly reduced in HL 60 / FAK cells , while expression of TRAIL receptor 2 and 3 , TRADD , and FADD was equivalent in both types of cells . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| TRAIL receptors 1 ( DR 4 ) and 2 ( DR 5 ) signal FADD dependent apoptosis and activate NF kappaB . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Finally , the expression of additional apoptosis inducing genes , including tumor necrosis factor receptor ( TNFR ) , FADD , TRAIL , and DR 5 , was detected in mammalian testis . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| FADD is required for DR 4 and DR 5 mediated apoptosis : lack of trail induced apoptosis in FADD deficient mouse embryonic fibroblasts . ^^^ To address this issue in a more physiological setting , we stably transfected mouse DR4 / 5 , human DR 4 , or human DR 5 into FADD ( / ) mouse embryonic fibroblast cells . ^^^ We conclude that FADD is required for DR 4 and DR 5 mediated apoptosis . . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Apo2L / TRAIL induced homomeric and heteromeric complexes of DR 4 and DR 5 and stimulated recruitment of FADD and caspase 8 and caspase 8 activation in nontransfected cells . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Both sensitive and resistant cell lines expressed TRAIL death receptor ( DR 5 ) , adapter protein Fas associated death domain ( FADD ) , and caspase 8 ; but resistant cell lines expressed 2 fold higher levels of the apoptosis inhibitor phosphoprotein enriched in diabetes / phosphoprotein enriched in astrocytes 15 kDa ( PED / PEA 15 ) . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| AZT and IFN alpha mediated apoptosis in PEL was blocked by stable overexpression of dominant negative Fas Associated Death Domain ( FADD ) , decoy receptor 2 ( DcR 2 ) , soluble TRAIL receptor fusion proteins ( DR 4 and DR 5 ) and thymidine . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| DAP 3 also associates with the pro caspase 8 binding adapter protein Fas associated death domain ( FADD ) , and links FADD to the TRAIL receptors DR 4 and DR 5 . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| As shown by semiquantitative reverse transcriptase polymerase chain reaction ( RT PCR ) , all PNET / medulloblastoma evaluated expressed DR 5 , the adapter protein FADD and caspase 3 , but only 48 % expressed caspase 8 . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| DR dependency was analyzed in FADD dominant negative or cytokine response modifier A overexpressing cells , and a possible role for DR 4 and DR 5 was investigated by antagonistic antibodies . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| IFN gamma or TRAIL treatment alone did not change expression of other pro or antiapoptotic proteins such as DR 4 , DR 5 , FADD , Bax , IAP 1 , XIAP , Bcl 2 , and Bcl XL . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| We show that by preventing DR 4 and DR 5 activation through expression of decoy receptor 1 ( DcR 1 ) and dominant negative FADD , we inhibit MEKK 1 induced apoptosis . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| PS 341 treatment by itself does not affect the levels of Bax , Bak , caspases 3 and 8 , c Flip or FADD , but elevates levels of TRAIL receptors DR 4 and DR 5 . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| DR 5 was internalized into the cytoplasm where it recruited FAS associated death domain protein ( FADD ) under TRAIL stimulation in both sensitive and resistant cells . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Direct binding of Fas associated death domain ( FADD ) to the tumor necrosis factor related apoptosis inducing ligand receptor DR 5 is regulated by the death effector domain of FADD . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Results from colocalization and immunoprecipitation studies showed that IG 20 can interact with TRAIL death receptors ( DR ) , DR 4 and DR 5 and increase recruitment of FADD and caspase 8 into the TRAIL death inducing signaling complex ( DISC ) . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Pretreating the cells with neutralizing antibodies to Fas , tumor necrosis factor ( TNF ) alpha receptor 1 , or TNF related apoptosis inducing ligand receptors ( DR 4 and DR 5 ) did not affect taxol induced apoptosis , but transfection of the cells with a dominant negative FADD plasmid resulted in inhibition of taxol induced apoptosis , revealing that taxol induces apoptosis independently of these death receptors but dependently on FADD . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Here , we show that in addition to the death domain , the C terminal tails of DR 4 and DR 5 positively regulate FADD binding , caspase activation and apoptosis . ^^^ Replacement of wild type or mutant DR 5 molecules into DR 5 deficient BJAB cells indicates that some agonistic antibodies display an absolute requirement for the C terminal tail for FADD binding and signaling while other antibodies can function in the absence of this mechanism . ^^^ These data demonstrate that regions outside the death domains of DR 4 and DR 5 have opposite effects to that of Fas in regulating FADD recruitment and show that different death receptor agonists can use distinct molecular mechanisms to activate signaling from the same receptor . . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Western blotting analyses revealed that IFN gamma dramatically increased the protein levels of interferon regulatory factor ( IRF ) 1 , but not TRAIL receptors ( DR 4 and DR 5 ) and pro apoptotic ( FADD and Bax ) and anti apoptotic factors ( Bcl 2 , Bcl XL , cIAP 1 , cIAP 2 and XIAP ) . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Extensive regions of the FADD death domain are required for binding to the TRAIL receptor DR 5 . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry was performed for cell cycle regulators [ p 53 , p 21 , p 27 , p 16 , cyclin D 1 , Rb ] , apoptosis related proteins [ Fas , Fas L , FADD , TRAIL , DR 4 , DR 5 , caspase 8 , caspase 3 , bcl 2 , Bax ] , tumor suppressor proteins [ beta catenin , E cadherin , FHIT , Smad 4 , VHL , PTEN , KAI 1 ] , and oncoproteins [ c myc , COX 2 , EGFR ] . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| Some members of the DR family , CD 95 and the TRAIL receptors DR 4 and DR 5 , directly bind FADD , whereas others , such as TNF receptor 1 and DR 3 , initially bind another adaptor protein , TRADD , which then recruits FADD . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| We have shown that COX 2 inhibition sensitizes human colon carcinoma cells to tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) induced apoptosis by inducing clustering of the TRAIL receptor DR 5 at the cell surface and the redistribution of the death inducing signaling complex components ( DR 5 , FADD , and procaspase 8 ) into cholesterol rich and ceramide rich domains known as caveolae . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| The synergistic effect is not associated with DR 4 , DR 5 , FADD , and FLIP ( L ) . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| RESULTS : CGH detected loss of the chromosomal regions that contain the following genes : 8p12 p 23 ( DR 4 and DR 5 ) , 2q33 34 ( caspase 8 ) , 11q13 . 3 ( FADD ) , 22q11 . 2 ( Bid ) , and 12q24 . 1 q24 . 3 ( Smac / DIABLO ) in TRAIL resistant cell lines . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| MG 132 treatment increases binding of DR 5 to the adaptor protein FADD , and causes caspase 8 activation and cleavage of pro apoptotic BID . ^^^ |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14763 and Q13158 |
Pubmed |
SVM Score :0.0 |
| NA |
|