| Interacting proteins: O14653 and Q13190 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O14653 and Q13190 |
Pubmed |
SVM Score :0.65552969 |
| Sed 5 is known to combine with two light chains ( Bos 1 and Sec 22 ) to form the t SNARE needed to receive vesicles from the endoplasmic reticulum . 0.65552969^^^ |
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| Interacting proteins: O14653 and Q13190 |
Pubmed |
SVM Score :0.0 |
| The proposed cis Golgi vesicle receptor syntaxin 5 was found in a complex with Golgi associated SNARE of 28 kDa ( GOS 28 ) , rbet 1 , rsly 1 , and two novel proteins characterized herein : rat sec22b and membrin , both cytoplasmically oriented integral membrane proteins . ^^^ The complex appears to recapitulate vesicle docking interactions of proteins originating from distinct compartments , since syntaxin 5 , rbet 1 , and GOS 28 localize to Golgi membranes , whereas mouse sec22b and membrin accumulate in the endoplasmic reticulum . ^^^ |
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| Interacting proteins: O14653 and Q13190 |
Pubmed |
SVM Score :0.0 |
| ER to Golgi transport , and perhaps intraGolgi transport involves a set of interacting soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) proteins including syntaxin 5 , GOS 28 , membrin , rsec22b , and rbet 1 . ^^^ However , all of the SNAREs were found on both COPII and COPI coated membranes , indicating that similar SNARE machinery directs both vesicle pathways . rsec22b and rbet 1 do not appear beyond the first Golgi cisterna , whereas syntaxin 5 and membrin penetrate deeply into the Golgi stacks . ^^^ Temperature shifts reveal that membrin , rsec22b , rbet 1 , and syntaxin 5 are present together on membranes that rapidly recycle between peripheral and Golgi centric locations . ^^^ By immunoprecipitation analysis , syntaxin 5 exists in at least two major subcomplexes : one containing syntaxin 5 ( 34 kD isoform ) and GOS 28 , and another containing syntaxin 5 ( 41 and 34 kD isoforms ) , membrin , rsec22b , and rbet 1 . ^^^ Our results indicate a central role for complexes among rbet 1 , rsec22b , membrin , and syntaxin 5 ( 34 and 41 kD ) at two membrane fusion interfaces : the fusion of ER derived vesicles with VTCs , and the assembly of VTCs to form cis Golgi elements . ^^^ |
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| Interacting proteins: O14653 and Q13190 |
Pubmed |
SVM Score :0.0 |
| Purified recombinant syntaxin 5 , membrin , and rbet 1 , three Q SNAREs , assemble cooperatively to create a high affinity binding site for sec22b , an R SNARE . ^^^ Interestingly , although sec22b binds to the combination of syntaxin 5 , membrin , and rbet 1 , it can only bind if it is present while the others assemble ; sec22b can not bind to a pre assembled ternary complex of syntaxin 5 , membrin , and rbet 1 . ^^^ |
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| Interacting proteins: O14653 and Q13190 |
Pubmed |
SVM Score :0.0 |
| This contrasts with a second distinct SNARE complex , also required for intra Golgi transport , consisting of syntaxin 5 ( Sed 5 ) membrin ( Bos 1 ) ERS 24 ( Sec 22 ) rBet 1 ( Bet 1 ) , whose 5 ( rBet 1 ) and t SNARE subunits ( membrin and ERS 24 ) , progressively decrease in concentration toward the trans face . ^^^ |
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| Interacting proteins: O14653 and Q13190 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that hsc 70 chaperones stimulate in vitro SNARE complex formation among the ER / Golgi SNAREs syntaxin 5 , membrin , rbetl and sec22b , under conditions in which assembly is normally inhibited . ^^^ |
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| Interacting proteins: O14653 and Q13190 |
Pubmed |
SVM Score :0.0 |
| To determine in which membranes protein function is required , temperature sensitive alleles of BOS 1 , BET 1 , SED 5 , SLY 1 , and YPT 1 that prevent ER / Golgi transport in vitro at restrictive temperatures were used to selectively inactivate these gene products on vesicles or on Golgi membranes . ^^^ |
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| Interacting proteins: O14653 and Q13190 |
Pubmed |
SVM Score :0.0 |
| In yeast , four integral membrane proteins , Sed 5 , Bos 1 , Sec 22 and Bet 1 ( refs 2 6 ) , each probably contribute a single helix to form the SNARE complex that is needed for transport from endoplasmic reticulum to Golgi . ^^^ Fusion only occurs when the 5 SNARE Bet 1 is on one membrane and the syntaxin heavy chain Sed 5 and its two light chains , Bos 1 and Sec 22 , are on the other membrane where they form a functional t SNARE . ^^^ |
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