| Interacting proteins: O14503 and O00327 |
Pubmed |
SVM Score :1.0073038 |
| Deletion analysis of DEC 1 demonstrated that its N terminal region , which includes the basic helix loop helix domain , was essential for both the suppressive activity and the interaction with BMAL 1 , as DEC 1 lacking the basic region did not show any suppression or interaction . 1.0073038^^^ These findings suggest that the basic region of DEC 1 participates in the transcriptional regulation through a protein protein interaction with BMAL 1 and DNA binding to the E box . . 0.73100528^^^ The interaction of DEC 1 with BMAL 1 has been suggested as one of the molecular mechanisms of the suppression [ Honma , S . , Kawamoto , T . , Takagi , Y . , Fujimoto , K . , Sato , F . , Noshiro , M . , Kato , Y . & Honma , K . ( 2002 ) Nature 419 , 841 844 ] . 0.52051026^^^ |
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| Interacting proteins: O14503 and O00327 |
Pubmed |
SVM Score :0.0 |
| In this study , we show robust and coordinated expression of circadian oscillator genes ( Npas 2 , Bmal 1 , Per 1 3 , and Cry 1 2 ) and clock controlled downstream genes ( Rev erb alpha , Rev erb beta , Dbp , E4bp4 , Stra 13 , and Id 2 ) in murine brown , inguinal , and epididymal ( BAT , iWAT , and eWAT ) adipose tissues . ^^^ |
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| Interacting proteins: O14503 and O00327 |
Pubmed |
SVM Score :0.0 |
| We found that Dec 1 and Dec 2 , basic helix loop helix transcription factors , repressed Clock / Bmal1 induced transactivation of the mouse Per 1 promoter through direct protein protein interactions with Bmal 1 and / or competition for E box elements . ^^^ |
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| Interacting proteins: O14503 and O00327 |
Pubmed |
SVM Score :0.0 |
| The expression of Per 1 , Per 2 , Cry 1 , Clock , Bmal 1 , Dec 1 , Dec 2 and c fos was determined 30 and 120 min after treatment in suprachiasmatic nucleus punches by real time reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ |
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| Interacting proteins: O14503 and O00327 |
Pubmed |
SVM Score :0.0 |
| DEC 1 and DEC 2 can inhibit CLOCK : BMAL 1 transactivation of the clock gene Per 1 , suggesting that these transcription factors may help regulate circadian timing . ^^^ |
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| Interacting proteins: O14503 and O00327 |
Pubmed |
SVM Score :0.0 |
| DNA binding , but not interaction with Bmal 1 , is responsible for DEC 1 mediated transcription regulation of the circadian gene mPer 1 . ^^^ In addition , DEC 1 ( R58P ) , a DNA binding mutant with Bmal 1 interactivity , repressed neither the mPer 1 reporter directly nor the Clock / Bmal1 induced activation , providing direct evidence that DNA binding , rather than Bmal 1 interactions , is responsible for the repression on the mPer 1 promoter . ^^^ |
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| Interacting proteins: O14503 and O00327 |
Pubmed |
SVM Score :0.0 |
| For this purpose , the 24 h expression of nine clock genes ( bmal 1 , clock , per 1 , per 2 , per 3 , cry 1 , cry 2 , dec 1 and dec 2 ) and the aa nat gene was monitored under light dark 8 : 16 and light dark 16 : 8 in the rat pineal by using real time RT PCR . ^^^ |
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| Interacting proteins: O14503 and O00327 |
Pubmed |
SVM Score :0.0 |
| Clock related genes including Per 1 , Per 2 , Cry 1 , Bmal 1 , Dec 1 , Dec 2 , Dbp , and Reverbalpha showed robust circadian expression rhythms in the submandibular glands in 12 : 12 hour light dark conditions . ^^^ The Clock mutation resulted in increased or decreased mRNA levels of Per 2 , Bmal 1 , Dec 1 , Dec 2 , and Dbp , and in Cry 1 / background , Cry 2 disruption also increased or decreased mRNA levels of these clock related genes and the amylase 1 gene . ^^^ |
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| Interacting proteins: O14503 and O00327 |
Pubmed |
SVM Score :0.0 |
| For this purpose , the 24 h profiles of nine clock genes ( bmal 1 , clock , per 1 , per 2 , per 3 , dec 1 , dec 2 , cry 1 and cry 2 ) and the arylalkylamine N acetyltransferase gene as an indicator of the circadian pacemaker output were compared between light dark periods of 8 : 16 and 16 : 8 h . ^^^ |
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