| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.83452685 |
| Shank directly interacts with GKAP and Homer , thus potentially bridging the N methyl D aspartate receptor PSD 95 GKAP complex and the mGluR Homer complex in synapses ( Naisbitt , S . , Kim , E . , Tu , J . 0.83452685^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| Shank , a novel family of postsynaptic density proteins that binds to the NMDA receptor / PSD 95 / GKAP complex and cortactin . ^^^ We report a novel family of postsynaptic density ( PSD ) proteins , termed Shank , that binds via its PDZ domain to the C terminus of PSD 95 associated protein GKAP . ^^^ A ternary complex of Shank / GKAP / PSD 95 assembles in heterologous cells and can be coimmunoprecipitated from rat brain . ^^^ Synaptic localization of Shank in neurons is inhibited by a GKAP splice variant that lacks the Shank binding C terminus . ^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| Binding partners for most of these domains have been identified : for instance , the PDZ domain of Shank proteins interacts with GKAP ( a postsynaptic density protein ) as well as several G protein coupled receptors . ^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| The PDZ domain of Shank binds to the C terminus of guanylate kinase associated protein ( GKAP ) . ^^^ The PDZ domain of Shank 1 from Rattus norvegicus and its complex with the C terminal octapeptide of GKAP were crystallized at 294 K using polyethylene glycol 20 000 and 6000 as precipitants . ^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| The overexpression of the mutant of the EVH 1 domain or deletion of the extreme C terminal leucine zipper motif markedly suppressed the synaptic localization of endogenous shank but not PSD 95 or GKAP . ^^^ In contrast , an overexpressed GKAP mutant lacking shank binding activity had no effect on the synaptic localization of shank . ^^^ Actin depolymerization by latrunculin A reduced the synaptic localization of PSD Zip 45 , shank , and F actin but not of PSD 95 or GKAP . ^^^ Thus , these data suggest that the PSD Zip 45 shank and PSD 95 GKAP complexes form different synaptic compartments , and PSD Zip 45 alone or PSD Zip 45 shank is involved in the synaptic accumulation of F actin . . ^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| SAPAP 1 also interacts with various proteins , including neurofilaments , synaptic scaffolding molecule ( S SCAM ) , nArgBP 2 , dynein light chain and Shank through different regions . ^^^ SAPAP 1 was not involved in the synaptic localization of PSD 95 or S SCAM , but affected that of Shank . ^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| Here we describe the crystal structures of Shank 1 PDZ in its peptide free form and in complex with the C terminal hexapeptide ( EAQTRL ) of guanylate kinase associated protein ( GKAP1a ) determined at 1 . 8 and 2 . 25 A resolutions , respectively . ^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| We investigated the molecular mechanisms of Shank 1 targeting and synapse assembly by looking at the function of guanylate kinase associated protein ( GKAP ) and PSD 95 interactions . ^^^ Shank 1 when it is not associated to GKAP , which binds to the Shank PSD 95 Discs Large zona occludens 1 domain , forms filamentous and fusiform structures in which the Src homology 3 domain specifically interacts with the ankyrin repeat domain , thus allowing its multimerization via a novel form of intermolecular interaction . ^^^ Surprisingly , in both COS 7 cells and hippocampal neurons , GKAP forms insoluble aggregates with Shank that colocalize with heat shock protein 70 and neurofilaments , two markers of the aggresomes in which misfolded proteins accumulate . ^^^ Synaptic activity in neurons induces the formation of Shank and GKAP intracellular aggregation and degradation . ^^^ Similarly , the overexpression of a GKAP mutant that is incapable of binding PSD 95 induces Shank aggregation and degradation in neurons . ^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| At maturity we calculated that a single excitatory postsynaptic site contains 100 450 of individual postsynaptic proteins , such as PSD 95 , GKAP , Shank and Homer . ^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| These mobile clusters contain the scaffolding proteins PSD 95 , GKAP , and Shank . ^^^ |
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| Interacting proteins: Q9UPX8 and O14490 |
Pubmed |
SVM Score :0.0 |
| We show here that four major scaffolding molecules , PSD 95 , GKAP , Shank , and PSD Zip 45 , show distinct instability in total molecular content per synapse . ^^^ Indeed , acute pharmacological disruption of F actin rapidly eliminated the dynamic fraction of GKAP , Shank , and PSD Zip 45 , without changing synaptic localization of PSD 95 . ^^^ GKAP content in synapses increased after pharmacological enhancement of neuronal activity , whereas Shank and PSD Zip 45 content showed reduction . ^^^ |
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