| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.54872405 |
| Furthermore , we found that DAP 1 is specifically expressed in the brain and colocalizes with PSD 95 and APC in mouse cerebellum . 0.54872405^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| We now report the isolation of a novel synaptic protein , termed GKAP for guanylate kinase associated protein , that binds directly to the GK domain of the four known members of the mammalian PSD 95 family . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| By yeast two hybrid screening , a novel protein termed guanylate kinase associated protein ( GKAP ) has been isolated that binds to the GK like domain of PSD 95 ( ) . ^^^ Characterization of guanylate kinase associated protein , a postsynaptic density protein at excitatory synapses that interacts directly with postsynaptic density 95 / synapse associated protein 90 . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Recently , novel classes of proteins interacting with the guanylate kinase domain of PSD 95 have been identified , guanylate kinase associated protein ( GKAP ) and SAP90 / PSD 95 associated proteins ( SAPAPs ) . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Activation of channel activity of the NMDA receptor PSD 95 complex by guanylate kinase associated protein ( GKAP ) . ^^^ PSD 95 also interacts with guanylate kinase associated protein ( GKAP ) through the guanylate kinase like domain of PSD 95 . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| PSD 95 also interacts with the hDLG associated protein DAP , which is also called Synapse associated protein 90 associated protein ( SAPAP ) , and Guanylate kinase associated protein ( GKAP ) . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Protein ligands for the PSD 95 guanylate kinase domain , guanylate kinase associated protein ( GKAP ) and MAP1A , appear not to interact with the canonical GMP binding pocket , and GMP binding does not influence the intramolecular SH3 / guanylate kinase ( GK ) interaction within PSD 95 . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| We investigated the molecular mechanisms of Shank 1 targeting and synapse assembly by looking at the function of guanylate kinase associated protein ( GKAP ) and PSD 95 interactions . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : SAPAP 1 was originally identified as a protein interacting with the guanylate kinase domain of PSD 95 . ^^^ The synaptic targeting of SAPAP 1 was mediated by the N terminal region and did not depend on the interaction with PSD 95 or S SCAM . ^^^ SAPAP 1 was not involved in the synaptic localization of PSD 95 or S SCAM , but affected that of Shank . ^^^ CONCLUSION : SAPAP 1 is a component of the static core of PSD , and its dynamics are different from those of the other PSD components , PSD 95 , S SCAM and BEGAIN . . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Five genes in the localities of the breakpoints have been identified as good candidates for the genetic basis of psychiatric illness in these families ; TTMA , a novel membrane spanning protein ; TCF 4 , a basic helix loop helix transcription factor ; DLGAP 1 , an interactor of the PSD 95 synaptic protein ; and ARKL 1 and ARKL 2 , novel members of the ubiquitin ligase gene family . . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| To determine their roles in the assembly of glutamatergic postsynaptic sites , we studied the distributions of NMDA and AMPA type glutamate receptors ; the NMDA receptor interacting proteins alpha actinin 2 , PSD 95 , and chapsyn ; and the PSD 95 associated protein GKAP during the development of hippocampal neurons in culture . ^^^ PSD 95 , chapsyn , and GKAP were present at some , but not all , nonsynaptic NMDA receptor clusters during the first 2 weeks , indicating that none is essential for NMDA receptor cluster formation . ^^^ When NMDA receptor clusters became synaptic , PSD 95 and GKAP were always present , consistent with an essential function in synaptic localization of NMDA receptors . ^^^ Furthermore , PSD 95 and GKAP clustered opposite presynaptic terminals several days before either NMDA or AMPA receptors clustered at these presumptive postsynaptic sites . ^^^ These results suggest that synapse development proceeds by formation of a postsynaptic scaffold containing PSD 95 and GKAP in concert with presynaptic vesicle clustering , followed by regulated attachment of glutamate receptor subtypes to this scaffold . . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Synaptic vesicle proteins , AMPA and NMDA type glutamate receptors , GABAA receptors , and the putative synapse organizing proteins PSD 95 , GKAP , and gephyrin formed numerous clusters at synaptic sites . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Shank , a novel family of postsynaptic density proteins that binds to the NMDA receptor / PSD 95 / GKAP complex and cortactin . ^^^ We report a novel family of postsynaptic density ( PSD ) proteins , termed Shank , that binds via its PDZ domain to the C terminus of PSD 95 associated protein GKAP . ^^^ A ternary complex of Shank / GKAP / PSD 95 assembles in heterologous cells and can be coimmunoprecipitated from rat brain . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Disrupting the CRIPT PSD 95 interaction in cultured hippocampal neurons with a PDZ 3 specific peptide prevented the association of PSD 95 with microtubules and inhibited the synaptic clustering of PSD 95 , chapsyn 110 / PSD 93 and GKAP ( a PSD 95 binding protein ) . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Mutations disrupting the intramolecular SH 3 GK interaction do not interfere with PSD 95 association with the K ( + ) channel Kv1 . 4 or with the GK domain binding protein GKAP . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate a direct interaction between the PSD 95 associated protein guanylate kinase domain associated protein ( GKAP ) and dynein light chain ( DLC ) , a light chain subunit shared by myosin 5 ( an actin based motor ) and cytoplasmic dynein ( a microtubule based motor ) . ^^^ A complex containing PSD 95 , GKAP , DLC , and myosin 5 can be immunoprecipitated from rat brain extracts . ^^^ We discuss the possibility that the GKAP / DLC interaction may be involved in trafficking of the PSD 95 complex by motor proteins . . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| The same was largely true for GKAP , a PSD 95 interacting protein . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| The overexpression of the mutant of the EVH 1 domain or deletion of the extreme C terminal leucine zipper motif markedly suppressed the synaptic localization of endogenous shank but not PSD 95 or GKAP . ^^^ Actin depolymerization by latrunculin A reduced the synaptic localization of PSD Zip 45 , shank , and F actin but not of PSD 95 or GKAP . ^^^ Thus , these data suggest that the PSD Zip 45 shank and PSD 95 GKAP complexes form different synaptic compartments , and PSD Zip 45 alone or PSD Zip 45 shank is involved in the synaptic accumulation of F actin . . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Pull down assay showed that r p 55 protein interacted with r p 55 itself and various PSD proteins , such as PSD 95 , SAP 97 , GKAP , CASK , GRIP , neuroligin , cadherin , tubulin , actin , alpha internexin , neurofilament L and Ca ( 2+ ) / calmodulin dependent protein kinase 2 , through its PDZ , SH 3 , HOOK or GK domains . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| At maturity we calculated that a single excitatory postsynaptic site contains 100 450 of individual postsynaptic proteins , such as PSD 95 , GKAP , Shank and Homer . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| These mobile clusters contain the scaffolding proteins PSD 95 , GKAP , and Shank . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| Bead induced direct aggregation of SALM 2 results in coclustering of PSD 95 and other postsynaptic proteins , including GKAP and AMPA receptors . ^^^ |
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| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| We show here that four major scaffolding molecules , PSD 95 , GKAP , Shank , and PSD Zip 45 , show distinct instability in total molecular content per synapse . ^^^ Among the PSD molecules examined , PSD 95 was most stable , but its elimination did not influence the dynamics of its direct binding partner GKAP . ^^^ Indeed , acute pharmacological disruption of F actin rapidly eliminated the dynamic fraction of GKAP , Shank , and PSD Zip 45 , without changing synaptic localization of PSD 95 . ^^^ |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14490 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|