| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| This initiation system requires in addition to the origin DNA sequences 1 ) Ad DNA polymerase ( Pol ) , 2 ) Ad preterminal protein ( pTP ) , the covalent acceptor for protein primed DNA replication , and 3 ) nuclear factor 1 ( NFI ) , a host cell protein identical to the CCAAT box binding transcription factor . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| A protein factor , CCAAT box binding transcription factor ( CTF ) , was isolated from HeLa nuclear extracts and shown to be responsible for stimulation of transcription in a reconstituted in vitro system . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| This region shows some similarity to the CTF NF 1 binding site [ Johnson and McKnight ( 1989 ) Annu . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The adenovirus origin of DNA replication is located within the terminal 51 bp of the viral genome and contains three recognizable domains : the minimal origin or `` core ' ' and binding sites for the cellular transcription factors NFI ( CTF ) and NFIII ( oct 1 , OTF 1 ) . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| One factor , previously identified as the mouse homologue of NF 1 ( or CTF ) , protects sequences 82 to 56 from exonuclease 3 digestion in vitro . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The first is a constitutive enhancer containing sequences homologous to the GT , Sph , and P motifs found in the SV 40 and polyomavirus enhancers ; others resemble the recognition sequence for CTF ( NF 1 ) , a factor which stimulates transcription of certain eukaryotic genes and replication of adenovirus DNA . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| Binding sites for CTF in the human Ha ras and alpha globin promoters were highly homologous to sequences recognized by nuclear factor 1 ( NF 1 ) , a cellular DNA binding protein that is required for the initiation of adenovirus DNA replication in vitro . ^^^ To determine the relationship between CTF and NF 1 , we compared the biochemical properties of these two proteins . ^^^ CTF and NF 1 were found to be indistinguishable in polypeptide composition , DNA binding properties , immunological cross reactivity , and in vitro stimulation of DNA replication and transcription initiation . ^^^ We conclude that CTF / NF 1 can serve both as a transcription selectivity factor for RNA polymerase 2 and as an initiation factor for adenovirus DNA replication . . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| CTF 5 a new transcriptional activator of the NFI / CTF family . ^^^ To define more precisely the role of the NFI / CTF family in regulating gene expression , we cloned the splice variant CTF 5 , analyzed transcriptional activation patterns in a yeast transcription assay , and compared it with other CTF proteins . ^^^ CTF 5 , which lacks exons 9 and 10 including a CTD like motif essential for transcriptional activation by full length CTF 1 , enhances transcription to a greater extent than CTF 1 . ^^^ In addition , CTF 5 is even more active than CTF 7 , which lacks exons 7 9 . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The purified protein also bound to the nuclear factor 1 ( NF 1 ) / CTF consensus sequence , albeit with lower affinity . ^^^ Gel mobility supershift and Western blotting ( immunoblotting ) analyses using an antibody against the NF1 / CTF protein identified the purified 32 and 34 kDa polypeptides as NF 1 or a related protein . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| Roles of the NFI / CTF gene family in transcription and development . ^^^ The Nuclear Factor 1 ( NFI ) family of site specific DNA binding proteins ( also known as CTF or CAAT box transcription factor ) functions both in viral DNA replication and in the regulation of gene expression . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The CCAAT binding transcription factor ( CTF ) / nuclear factor 1 ( NF 1 ) group of cellular DNA binding proteins recognizes the sequence GCCAAT and is implicated in eukaryotic transcription , as well as DNA replication . ^^^ Molecular analysis of human CTF / NF 1 cDNA clones revealed multiple mRNA species that contain alternative coding regions , apparently as a result of differential splicing . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| In the present study , we isolated multiple cDNAs encoding a family of NFI , NFI B , from mouse brain cDNA libraries . ^^^ The entire sequence of NFI B suggests that NFI B is a mouse counterpart of NFI L , which was previously isolated from rat liver . ^^^ NFI B is highly enriched in the cerebellum , in contrast with a ubiquitous NFI , CTF / NF 1 . ^^^ NFI B proteins translated in vitro could bind to the MBP NFI site . ^^^ Furthermore , we demonstrated by transfection experiments that NFI B activated transcription from transfected MBP promoter in neuronal / glial NG 108 15 cells . . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| Chicken NFI / TGGCA proteins are encoded by at least three independent genes : NFI A , NFI B and NFI C with homologues in mammalian genomes . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The four nuclear factor 1 genes ( NFI A , NFI B , NFI C , and NFI 10 ) give rise to multiple isoforms by alternative splicing in many tissues . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| It is known that nuclear factor 1 ( NF 1 ) family proteins are encoded by at least four independent genes in vertebrates , and NF 1 L and NF1 / Red1 are derived from different genes , NFI A and NFI B , respectively . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| NFI B 3 , a novel transcriptional repressor of the nuclear factor 1 family , is generated by alternative RNA processing . ^^^ Nuclear factor 1 ( NFI ) proteins constitute a family of sequence specific transcription factors whose functional diversity is generated through transcription from four different genes ( NFI A , NFI B , NFI C , and NFI 10 ) , alternative RNA splicing , and protein heterodimerization . ^^^ Here we describe a naturally truncated isoform , NFI B 3 , which is derived from the human NFI B gene , in addition to characterizing further human NFI B 1 and NFI B 2 , two differentially spliced variants previously isolated from hamster and chicken . ^^^ Although NFI B 1 and NFI B 2 proteins are translated from an 8 . 7 kilobase message , the mRNA for NFI B 3 has a size of only 1 . 8 kilobases . ^^^ The NFI B 3 message originates from the failure to excise the first intron downstream of the exons encoding the DNA binding domain and subsequent processing of this transcript at an intron internal polyadenylation signal . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| This family consists of four subtypes , NFI A , NFI B , NFI C , and NFI 10 , each encoded by a different gene . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The relative activation potentials for the NFI isoforms differed between the two promoters , with NFI 10 being the strongest activator of NFI Ad and NFI B being the strongest activator of the MMTV promoter . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| Expression of NFI C or NFI 10 , but not NFI A or NFI B proteins , represses glucocorticoid induction of the MMTV promoter in HeLa cells . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The four genes exhibit unique but overlapping patterns of expression during embryonic development , with high level expression of NFI A , NFI B , and NFI 10 transcripts in neocortex and extensive expression of the four genes in muscle , connective tissue , liver , and other organ systems . ^^^ The four NFI gene products studied differ in their ability to activate expression of the NFI dependent MMTV promoter , with the NFI B protein being most active and the NFI A protein being least active . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| To determine the chromosomal position of NFI genes in the human genome , we isolated partial cDNA sequences derived from four independent genes : NFIA , NFIB , NFIC , and NFIX . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The four murine NFI genes are on Chrs 4 ( Nfia and Nfib ) , 8 ( Nfix ) , and 10 ( Nfic ) , suggesting early duplication of the genes and dispersal throughout the genome . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| Nuclear factor 1 ( NF 1 ) family proteins , which are encoded by four different genes ( NF 1 A , NF 1 B , NF 1 C and NF 1 10 ) , bind to the palindromic sequence and regulate the expression of many viral and cellular genes . ^^^ We have previously purified NF 1 A and NF 1 B from rat liver as factors that bind to the silencer in the glutathione transferase P gene , and have also reported the repression domain of NF 1 A . ^^^ The DNA binding affinities of NF 1 A and NF 1 10 are higher than those of NF 1 B and NF 1 C , whereas all four NF 1 proteins showed the same DNA binding specificity . ^^^ Transfection analyses revealed that the function of NF 1 B on the transcriptional regulation differed between NF 1 B isoforms and was affected by the factor ( s ) that bind to the promoter regions . ^^^ In addition , we identified the transcriptional regulatory domain of NF 1 B , which is enriched with proline and serine residues . . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| This study reports the characterization of NFI gene expression during mammary gland development and the identification and cloning of specific NFI isoforms ( NFI A 4 , NFI B 2 , and NFI X 1 ) from the mouse mammary gland during lactation . ^^^ On both the WAP CoRE and the mouse mammary tumor virus long terminal repeat promoter , the NFI B isoform preferentially activated gene transcription in cooperation with STAT5A and GR . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| No mutations were found in these genes ; however , unexpectedly , increased expression of the transcription factor NFI B was detected in granulocytes and CD 34 ( + ) cells in PV with 9pLOH . ^^^ Since a member of the NFI gene family ( NFI 10 ) was reported to result in TGF beta resistance when overexpressed in vitro ( TGF beta is a known inhibitor of hematopoiesis ) , we transfected the NFI B gene to the mouse 32D cell line . ^^^ We found that overexpression of the NFI B gene confers TGF beta resistance in vitro . ^^^ Analysis of genes within this 9pLOH region revealed increased expression of the NFI B gene . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The NFI family is encoded by four distinct genes , NFI A , NFI B , NFI C , and NFI 10 . ^^^ Only trace amounts of NFI B and NFI 10 were detectable in rat liver and cultured hepatocytes . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The binding site for this factor was identified by footprinting and turned out to be closely related to the previously described recognition site for the TGGCA binding protein , the chicken analog of nuclear factor 1 ( NF 1 ) . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| We showed previously that the four murine NFI genes ( Nfia , Nfib , Nfic , and Nfix ) are expressed in unique but overlapping patterns during mouse development and in adult tissues . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| All four NFI genes are expressed in embryonic mouse brain , with Nfia , Nfib , and Nfix being expressed highly in developing cortex ( Chaudhry et al . , 1997 ) . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| Four genes encoding nuclear factor 1 ( NFI ) transcription replication proteins are present in the mouse genome : Nfia , Nfib , Nfic , and NFIX : In order to elucidate its physiological role ( s ) , we disrupted the Nfic gene in mice . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| The nuclear factor 1 ( NF 1 ) family proteins are encoded by four different genes ( Nfia , Nfib , Nfic and Nfix ) and regulate gene expression and DNA replication . ^^^ In this study , we determined the genomic organization and nucleotide sequences of the exon / intron boundaries of the rat Nfib , Nfic and Nfix genes in silico . ^^^ |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00712 and P08651 |
Pubmed |
SVM Score :0.0 |
| NA |
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