| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| In association with two other ( single transmembrane domained ) membrane proteins , UPII and UPIII , UPIa and UPIb form 16 nm particles that naturally form two dimensional crystalline arrays , thus providing unique opportunities for studying membrane structure and function . ^^^ We show here that UPIa and UPIb , which share 39 % of their amino acid sequence , are cross linked to UPII and UPIII , respectively . ^^^ We also show that UPIa has a propensity to oligomerize , forming complexes that are stable in SDS , and that UPII can be readily cross linked to form homodimers . ^^^ These data suggest that there exist two types of 16 nm AUM particles that contain UPIa / UPII or UPIb / UPIII , and support a model in which the UPIa and UPII occupy the inner and outer domains , respectively , of the UPIa / UPII particle . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| Studies of the protease sensitivity of the different subdomains of uroplakins and other considerations suggest that UPIa and UPIb have 4 transmembrane domains , while UPII and UPIII have only one transmembrane domain . ^^^ Chemical crosslinking studies showed that UPIa and UPIb , which share 39 % amino acid sequence , are topologically adjacent to UPII and UPIII , respectively , thus raising the possibility that there exist two biochemically distinct AUM particles , i . e . , those containing UPIa / UPII vs . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| Uroplakin gene expression by normal and neoplastic human urothelium . cDNA sequences for human uroplakins UPIa , UPIb , UPII , and UPIII were cloned and used to investigate uroplakin transcription by normal and neoplastic urothelial cells . ^^^ By in situ hybridization , UPIa and UPII were confined to superficial cells and UPIb was also expressed by intermediate cells . ^^^ UPIa and UPII were detected superficially only in well differentiated transitional cell carcinoma papillae . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| When uroplakins are solubilized with 2 % octylglucoside and fractionated with ion exchangers , UPIa and UPII were bound as a complex by a cation exchanger , whereas UPIb and UPIII were bound by an anion exchanger . ^^^ This result is consistent with the fact that UPIa and UPIb are cross linked to UPII and UPIII , respectively , and suggests that the four uroplakins form two pairs consisting of UPIa / II and UPIb / III . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| This study investigated the expression of the four uroplakin genes , UPIa , UPIb , UPII and UPIII , in a wide range of normal human tissues to determine tissue specificity and in advanced transitional cell carcinoma ( TCC ) to examine gene expression in primary and metastatic disease . ^^^ UPIa and UPII appeared to be urothelium specific , but UPIb was detected in several non urothelial tissues , including the respiratory tract , where it was associated with squamous metaplasia of tracheal and bronchial epithelia . ^^^ These results confirm that in human tissues the expression of UPIa and UPII genes is highly specific to urothelium and suggest that the tight differentiation restricted expression of uroplakin genes in normal urothelium is lost following malignant transformation . . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| Transfection experiments showed that , when expressed individually , only UPIb can exit from the endoplasmic reticulum ( ER ) and move to the plasma membrane , whereas UPII and UPIII reach the plasma membrane only when they form heterodimeric complexes with UPIa and UPIb , respectively . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| Two high affinity activators of PPARgamma , troglitazone ( TZ ) and rosiglitazone ( RZ ) induced the expression of mRNA for UPII and UPIb and , to a lesser extent , UPIa . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| We determined the sensitivity and specificity of each individual marker and the combinations of UPIa / UPII and UPIb / UPIII . ^^^ The latter strategy was based on our data , which showed that UPIa and UPIb form heterodimers with UPII and UPIII , respectively . ^^^ All 8 patients were positive at presentation for UPIa / UPII . ^^^ The combination of UPIa / UPII provided the best sensitivity ( 75 % ) of detecting CTCs , with a specificity of 50 % . ^^^ Detection of EGFR positive cells alone and in combination with UPs was inferior to that for UPIa / UPII . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| Urothelial umbrella cells are characterized by apical , rigid membrane plaques , which contain four major uroplakin proteins ( UP Ia , Ib , 2 and 3 ) forming UPIa / UPII and UPIb / UPIII pairs . ^^^ We stained immunohistochemically ( ABC peroxidase method ) paraffin sections of normal human ureter ( n = 18 ) and urinary bladder ( n = 10 ) using rabbit antibodies against UPIa , UPIb , UPII and UPIII ; a recently raised mouse monoclonal antibody ( MAb ) , AU 1 , and two new MAbs , AU 2 and AU 3 , all against UPIII ; and mouse MAbs against umbrella cell associated cytokeratins CK 18 and CK 20 . ^^^ The UPIb / UPIII negative cells showed either normal or reduced amounts of UPIa and UPII staining . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| We have shown previously that bovine AUMs contain four major integral membrane proteins , i . e . , uroplakins Ia , Ib , 2 , and IIIa , and that UPIa and Ib ( both tetraspanins ) form heterodimers with UPII and IIIa , respectively . ^^^ Using a panel of antibodies recognizing different conformational states of uroplakins , we demonstrate that the UPIa dependent , furin mediated cleavage of the prosequence of UPII leads to global conformational changes in mature UPII and that UPIb also induces conformational changes in its partner UPIIIa . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| METHODS : We used the urothelium of anuric patients on long term hemodialysis , sampled at the time of renal transplantation , to investigate the expression of urothelial differentiation associated antigens , including uroplakins ( UPIa , UPIb , UPII , and UPIIIa ) , cytokeratin isotypes ( CK 7 , CK 8 , CK 13 , CK 14 , CK 17 , CK 18 , and CK 20 ) , nuclear hormone receptors [ peroxisome proliferators activated receptor gamma ( PPAR gamma ) and retinoid 10 receptor alpha ( RXR alpha ) ] , and a cell cycle marker ( Ki 67 ) . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| The drastically different carbohydrate processing of the UPIa and UPIb proteins , two closely related members of the tetraspanin family , may reflect differences in their folding and masking due to their interactions with their associated proteins , UPII and UPIIIa , respectively . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| Genes for uroplakins IA , IB , and 2 were mapped to bovine ( BTA ) Chromosomes ( Chrs ) 18 ( UPK1A ) , 1 ( UPK1B ) , and 15 ( UPK 2 ) , respectively . ^^^ We have also mapped genes for uroplakins IA and 2 in mice , to the proximal regions of mouse Chr 7 ( Upk1a ) and 9 ( Upk 2 ) , respectively , by analyzing the inheritance of restriction fragment length variants in recombinant inbred mouse strains . ^^^ |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00526 and O00322 |
Pubmed |
SVM Score :0.0 |
| NA |
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