| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.84037107 |
| Menin , the 610 amino acid nuclear protein encoded by MEN 1 , binds to the transcription factor JunD and can repress JunD induced transcription . 0.84037107^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.85027226 |
| Menin , a nuclear protein encoded by the tumor suppressor gene MEN 1 , interacts with the AP 1 transcription factor JunD and inhibits its transcriptional activity . 0.85027226^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin interacts with the AP 1 transcription factor JunD and represses JunD activated transcription . ^^^ Using a yeast two hybrid screen with menin as the bait , we have identified the transcription factor JunD as a direct menin interacting partner . ^^^ The menin JunD interaction was confirmed in vitro and in vivo . ^^^ Menin repressed transcriptional activation mediated by JunD fused to the Gal 4 DNA binding domain from a Gal 4 responsive reporter , or by JunD from an AP 1 responsive reporter . ^^^ Several naturally occurring and clustered MEN 1 missense mutations disrupted menin interaction with JunD . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin is principally a nuclear protein ; menin interacts with junD . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin represses JunD activated transcription by a histone deacetylase dependent mechanism . ^^^ Here we report that menin functions as a transcriptional repressor through interaction with the transcription factor JunD . ^^^ The interaction is mediated via the N terminal transcription activation domain of JunD , and the C terminal part of menin . ^^^ In transient co transfection experiments , expression of menin leads to specific repression of JunD transcriptional activity , which is dependent on the integrity of the menin C terminal region . ^^^ This would result in loss of repression of menin / JunD target genes , as well as non target genes through indirect mechanisms , deregulation of cellular growth control and endocrine tumorigenesis . . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin binds to JunD , an AP 1 transcription factor , inhibiting JunD ' s activation of transcription . ^^^ |
|
| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| In comparison with the recent functional studies of the menin protein interactions , this mutation is located in a region with little or no binding activity to JunD and activating protein 1 transcription factor . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| In 1997 the responsible tumour suppressor gene was identified and recently it was shown that menin , its encoded protein , represses JunD activated gene expression . ^^^ Since the deleted region of menin has been implicated in binding to JunD , this may explain the tumourigenic effect of this mutation . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| The protein encoded by MEN 1 , 610 amino acid menin , resides primarily in the nucleus and binds to the transcription factor JunD , resulting in the repression of JunD induced transcription . ^^^ Zebrafish menin binds both human and mouse JunD , and represses JunD induced transcription , indicating that the JunD binding ability of menin is evolutionarily conserved . . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| JunD appears to be one mediator of this effect , since JunD was a major component of the AP 1 DNA binding complex in granulosa cells , and menin , a selective inhibitor of JunD , blocked transcription of 73COL luciferase . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| The MEN 1 gene encodes a putative growth suppressor protein , menin , binding JunD , a transcriptional factor belonging to the AP 1 complex . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Identification and characterization of JunD missense mutants that lack menin binding . ^^^ Menin , the product of the MEN 1 tumor suppressor gene , binds to the AP 1 transcription factor JunD and represses JunD transcriptional activity . ^^^ The effects of human or mouse JunD missense mutations upon menin interaction were studied by random and alanine scanning mutagenesis of the menin binding region of JunD ( amino acids 1 70 ) . ^^^ JunD mutant proteins were tested for menin binding in a reverse yeast two hybrid assay , and for transcriptional regulation by menin in AP 1 reporter assays . ^^^ Random mutagenesis identified two different mutations that disrupted menin interaction at mouse JunD amino acid 42 ( G42E and G42R ) . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Differential binding of the Menin tumor suppressor protein to JunD isoforms . ^^^ Here we report the first characterized functional difference between these JunD isoforms ; only the full length isoform of JunD binds to the Menin tumor suppressor protein . ^^^ Furthermore , Menin suppresses transcriptional activity of the full length but not the truncated isoform of JunD , which identifies the full length JunD isoform as a functional target of Menin . . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| The MEN 1 gene encodes menin , a nuclear protein interacting with JunD / AP1 , Smad 3 , NFkappaB , and other proteins involved in transcription and cell growth regulation . ^^^ Taken together , most missense and in frame MEN 1 genomic alterations affect one or all domains of menin interacting with JunD [ codons 1 40 ; 139 242 ; 323 428 ] , Smad 3 [ distal to codon 478 ] , and NFkappaB [ codons 276 479 ] , three major effectors in transcription and cell growth regulation . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Its protein product , called menin , has been shown to associate with the AP 1 transcription factor JunD and to repress JunD mediated transcription . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin has been shown negatively to regulate transcriptional activation mediated by JunD , although the significance of this interaction in normal cell physiology and how the absence of menin leads to tumorigenesis are unknown . ^^^ The role of menin at meiotic telomeres appears to be independent of JunD and may not have a counterpart in somatic cells . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| The MEN 1 gene product menin may have a role in transcriptional regulation involving JunD ; several other menin interacting proteins have also been identified . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Proteins of known function are shown to interact with Menin : JunD , nuclear factor KappaB , Smad 3 , Pem , Nm23H1 , glial fibrillary acidic protein , Vimentin , and probably P 53 . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| The translation product of the MEN 1 gene , menin , has been reported to suppress JunD mediated activator protein 1 ( AP 1 ) transactivation and inhibit Ras mediated tumor formation , but its molecular mechanisms and physiologic significance have been poorly elucidated . ^^^ Menin did not alter the expression levels of AP 1 proteins except c Fos , phosphorylation of c Jun and JunD and DNA binding properties of AP 1 proteins . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin binds and alters JunD , NF kappaB , Smad 3 mediated transcriptional activation . ^^^ |
|
| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Transcription factor JunD , deprived of menin , switches from growth suppressor to growth promoter . ^^^ Also , in prior studies , JunD alone bound menin , product of the MEN 1 tumor suppressor gene , and JunD ' s transcriptional activity was inhibited by menin , suggesting that JunD might achieve all or most of its unique properties through binding to menin . ^^^ Analyses of JunD and menin effects on proliferation , morphology , and cyclin D 1 in stable cell lines unmasked an unexpected growth promoting activity of JunD . ^^^ Whereas stable overexpression of wild type ( wt ) mouse JunD in JunD / immortalized fibroblasts inhibited their proliferation and reverted their transformed like phenotype , overexpression of a missense mouse JunD mutant ( mJunDG42E ) with disabled binding to menin showed opposite or growth promoting effects . ^^^ To conclude , JunD changed from growth suppressor to growth promoter when its binding to menin was prevented by a JunD mutant unable to bind menin or by Men 1 null genetic background . . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin , a tumor suppressor , represses JunD mediated transcriptional activity by association with an mSin3A histone deacetylase complex . ^^^ Menin , a gene product of multiple endocrine neoplasia type 1 ( MEN 1 ) , is known to act as a tumor suppressor to repress JunD transcription factor . ^^^ However , the mechanism by which Menin represses JunD transcriptional activity was still unclear . ^^^ In this study , we found that Menin is a corepressor against JunD transcriptional activity via recruitment of histone deacetylases in an mSin3A dependent manner . ^^^ The SID mutation of Menin ( L381P / A385P ) abolished the interaction between mSin3A and paired amphipathic helix 2 domain of Menin and reduced its ability to repress JunD transcriptional activity , implicating that SID of Menin is important for recruiting an mSin3A histone deacetylase complex to repress JunD transcriptional activity . . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| JunD was up regulated in menin expressing clones , whereas delta like protein 1 / preadipocyte factor 1 ( involved in differentiation and growth of the pancreatic endocrine cells ) , proliferating cell nuclear antigen , and QM / Jif 1 ( a negative regulator of c Jun ) became down regulated . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin suppresses osteoblast differentiation by antagonizing the AP 1 factor , JunD . ^^^ The activator protein 1 ( AP 1 ) transcription factor , JunD , is expressed in osteoblasts and has been shown to interact with menin in other cell types . ^^^ Here , we examined the consequences of menin JunD interaction on osteoblast differentiation in mouse osteoblastic MC3T3 E 1 cells . ^^^ In MC3T3 E 1 cells in which menin expression was reduced by stable menin antisense DNA transfection , JunD levels were increased . ^^^ When JunD and menin were co transfected in MC3T3 E 1 cells , they co immunoprecipitated . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| The negative regulators of AP 1 , JunD , and Menin were decreased in tumor mucosa . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| In vitro studies have shown menin binding to JunD , Pem , Smad 3 , NF kappaB , nm23H1 , and RPA 2 proteins . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Inactivation of menin switches its JunD partner from a downstream action of growth suppression to growth promotion . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin also suppresses osteoblast maturation , partly by inhibiting the differentiation actions of JunD . . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Menin represses JunD transcriptional activity in protein kinase C theta mediated Nur 77 expression . ^^^ Menin is identified as the transcriptional corepressor for JunD via recruitment of mSin 3 istone deacetylases . ^^^ In fact , Menin represses PKCtheta / p300 mediated transcriptional activity of JunD in T cell . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| Recent observations indicate several functions for menin in the regulation of transcription , serving either as a repressor or as an activator : menin interacts with the activator protein 1 family transcription factor JunD , changing it from an oncoprotein into a tumor suppressor protein , putatively by recruitment of histone deacetylase complexes ; menin maintains transforming growth factor beta mediated signal transduction involved in parathyroid hormone and prolactin gene expression ; and menin is an integral component of histone methyltransferase complexes . ^^^ |
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| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00255 and P17535 |
Pubmed |
SVM Score :0.0 |
| NA |
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