| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| We examined the roles of yeast Ccc 2 , a P type ATPase related to human ATP7A ( Menkes disease protein ) and ATP7B ( Wilson disease protein ) , as well as yeast Atx 1 , a cytosolic copper chaperone . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| An important step in copper homeostasis is delivery of copper to a specific P type ATPase in the Golgi apparatus ( Ccc 2 in yeast , ATP7A and ATP7B in humans ) by a small copper chaperone protein ( Atx 1 in yeast , ATOX 1 in humans ) . ^^^ Atx 1 and ATOX 1 both contain an MXCXXC motif that is also present in Ccc 2 ( two motifs ) and ATP7A / B ( six motifs ) . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| ATOX 1 is a cytoplasmic copper chaperone that interacts with the copper binding domain of the membrane copper transporters ATP7A and ATP7B . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| We screened for ATOX 1 mutations in two patients with classical Menkes disease phenotypes and one individual with occipital horn syndrome who had no alterations detected in ATP7A , as well as an adult female with chronic anemia , low serum copper and evidence of mild dopamine beta hydroxylase deficiency and no alterations in the ATOX 1 coding or splice junction sequences were found . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| Attempts to monitor a direct Atox 1 MNK interaction and to determine kinetic parameters have not been successful . ^^^ Here we investigated interactions of Atox 1 with wild type and mutated pairs of the MBSs of MNK using two different methods : yeast two hybrid analysis and real time surface plasmon resonance ( SPR ) . ^^^ A copper dependent interaction of Atox 1 with the MBSs of MNK was observed by both approaches . ^^^ Therefore , a functional difference of the MBSs in the MNK N terminus can not be attributed to cooperativity effects or varying affinities of the copper chaperone Atox 1 with the MBSs . . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| Atox 1 delivers copper to the secretory pathway and docks with either copper transporting ATPase ATP7B in the liver or ATP7A in other cells . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| In all tissues examined , mRNA expression for CTR 1 , ATOX 1 , ATP7A , and ATP7B was unchanged by iron deficiency . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| We use yeast and mammalian two hybrid systems , along with an in vitro assay to demonstrate a specific , copper dependent interaction between the six metal binding domains of the WD and MNK ATPases and the cytoplasmic copper chaperone HAH 1 . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| On Western blot analysis all three resistant lines exhibited increased expression of one or the other of the two copper export pumps ( ATP7A or ATP7B ) with no change in the HAH 1 chaperone . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| The interaction between the human copper ( 1 ) chaperone , HAH 1 , and one of its two physiological partners , the Menkes disease protein ( ATP7A ) , was investigated in solution using heteronuclear NMR . ^^^ The study was carried out through titrations involving HAH 1 and either the second or the fifth soluble domains of ATP7A ( MNK 2 and MNK 5 , respectively ) , in the presence of copper ( 1 ) . ^^^ In particular , no stable adduct is formed between either of the MNK domains and HAH 1 . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| The interaction in vitro with the physiological ATP7A copper ( 1 ) donor ( HAH 1 ) was additionally studied . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| A NMR study of the interaction of a three domain construct of ATP7A with copper ( 1 ) and copper ( 1 ) HAH 1 : the interplay of domains . ^^^ ATP7A receives copper from a soluble protein , the metallochaperone HAH 1 . ^^^ |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00244 and Q762B6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| On Western blot analysis all three resistant lines exhibited increased expression of one or the other of the two copper export pumps ( ATP7A or ATP7B ) with no change in the HAH 1 chaperone . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| We examined the roles of yeast Ccc 2 , a P type ATPase related to human ATP7A ( Menkes disease protein ) and ATP7B ( Wilson disease protein ) , as well as yeast Atx 1 , a cytosolic copper chaperone . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| We screened for ATOX 1 mutations in two patients with classical Menkes disease phenotypes and one individual with occipital horn syndrome who had no alterations detected in ATP7A , as well as an adult female with chronic anemia , low serum copper and evidence of mild dopamine beta hydroxylase deficiency and no alterations in the ATOX 1 coding or splice junction sequences were found . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| Atox 1 delivers copper to the secretory pathway and docks with either copper transporting ATPase ATP7B in the liver or ATP7A in other cells . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| In all tissues examined , mRNA expression for CTR 1 , ATOX 1 , ATP7A , and ATP7B was unchanged by iron deficiency . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| An important step in copper homeostasis is delivery of copper to a specific P type ATPase in the Golgi apparatus ( Ccc 2 in yeast , ATP7A and ATP7B in humans ) by a small copper chaperone protein ( Atx 1 in yeast , ATOX 1 in humans ) . ^^^ Atx 1 and ATOX 1 both contain an MXCXXC motif that is also present in Ccc 2 ( two motifs ) and ATP7A / B ( six motifs ) . ^^^ Atx 1 and ATOX 1 both contain an MXCXXC motif that is also present in Ccc 2 ( two motifs ) and ATP7A / B ( six motifs ) . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| ATOX 1 is a cytoplasmic copper chaperone that interacts with the copper binding domain of the membrane copper transporters ATP7A and ATP7B . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| The interaction between the human copper ( 1 ) chaperone , HAH 1 , and one of its two physiological partners , the Menkes disease protein ( ATP7A ) , was investigated in solution using heteronuclear NMR . ^^^ The study was carried out through titrations involving HAH 1 and either the second or the fifth soluble domains of ATP7A ( MNK 2 and MNK 5 , respectively ) , in the presence of copper ( 1 ) . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| The interaction in vitro with the physiological ATP7A copper ( 1 ) donor ( HAH 1 ) was additionally studied . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| A NMR study of the interaction of a three domain construct of ATP7A with copper ( 1 ) and copper ( 1 ) HAH 1 : the interplay of domains . ^^^ ATP7A receives copper from a soluble protein , the metallochaperone HAH 1 . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| We use yeast and mammalian two hybrid systems , along with an in vitro assay to demonstrate a specific , copper dependent interaction between the six metal binding domains of the WD and MNK ATPases and the cytoplasmic copper chaperone HAH 1 . ^^^ |
|
| Interacting proteins: Q762B6 and O00244 |
Pubmed |
SVM Score :0.0 |
| Attempts to monitor a direct Atox 1 MNK interaction and to determine kinetic parameters have not been successful . ^^^ Here we investigated interactions of Atox 1 with wild type and mutated pairs of the MBSs of MNK using two different methods : yeast two hybrid analysis and real time surface plasmon resonance ( SPR ) . ^^^ A copper dependent interaction of Atox 1 with the MBSs of MNK was observed by both approaches . ^^^ Therefore , a functional difference of the MBSs in the MNK N terminus can not be attributed to cooperativity effects or varying affinities of the copper chaperone Atox 1 with the MBSs . . ^^^ |
|