| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.88591869 |
| These data demonstrate that rat SNAP 23 associates with syntaxin 4 before insulin stimulation and is present in the SNARE complexes known to mediate the translocation of GLUT 4 from intracellular vesicles to the plasma membrane of rat adipose cells . . 0.88591869^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.52099544 |
| These results suggest that the interaction between syntaxin 4 , SNAP 23 and VAMP 2 is fairly weak and their concentrations in the cell lysate are insufficient to make a readily detectable complex , and that bindings between these proteins are hindered by other proteins in parotid acinar cells . . 0.52099544^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.77580002 |
| Within the syntaxin family , syntaxin 4 interacted with SNAP 23 and all vesicle associated membrane proteins ( VAMPs ) examined , except tetanus neurotoxin insensitive VAMP ( TI VAMP ) . 0.77580002^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Inhibition of the binding of SNAP 23 to syntaxin 4 by Munc18c . ^^^ Expression of SNAP 23 , syntaxin 4 , and the syntaxin binding protein Munc 18c in COS cells revealed that Munc18c reduced the amount of SNAP 23 bound to syntaxin 4 in a concentration dependent manner . ^^^ These results suggest that the binding of SNAP 23 to syntaxin 4 is inhibited by Munc18c in adipocytes . . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Syntaxin 4 , SNAP 23 and / or SNAP 25 are also implicated in this process . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| This is the first localization of SNAP 23 within a polarized tissue and suggests that this t SNAREs may interact with syntaxin 4 to mediate basolateral secretion . . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Binary interactions of the SNARE proteins syntaxin 4 , SNAP 23 , and VAMP 2 and their regulation by phosphorylation . ^^^ In this study , we defined the binding characteristics of three SNARE proteins : SNAP 23 , VAMP 2 , and syntaxin 4 . ^^^ Binary , saturable interactions among all three partners ( VAMP 2 syntaxin 4 , VAMP 2 SNAP 23 , and SNAP 23 syntaxin 4 ) were measured in vitro . ^^^ Unlike its neuronal counterpart , SNAP 23 did not potentiate VAMP 2 binding to its putative t SNARE partner , syntaxin 4 . ^^^ Importantly , PKA but not casein kinase 2 phosphorylation of syntaxin 4 disrupted its binding to SNAP 23 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Roles of vesicle associated membrane protein , SNAP 23 , and syntaxin 4 . ^^^ To understand the molecular basis of granule release from platelets , we examined the role of vesicle associated membrane protein , SNAP 23 , and syntaxin 4 in alpha granule secretion . ^^^ A vesicle associated membrane protein , SNAP 23 , and syntaxin 4 were detected in platelet lysate . ^^^ These results show that vesicle associated membrane protein , SNAP 23 , and syntaxin 4 form a heterotrimeric complex in platelets that disassembles with activation and demonstrate that alpha granule release is dependent on vesicle SNAP receptor target SNAP receptor ( vSNARE tSNARE ) interactions . . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| VAMP 2 and VAMP3 / cellubrevin ( 5 SNARE ' s ) have been shown to interact with the t SNARE ' s syntaxin 4 and SNAP 23 . ^^^ Furthermore , recent studies have demonstrated that VAMP2 / 3 , syntaxin 4 , SNAP 23 , and NSF are functionally involved in insulin stimulated GLUT 4 translocation in adipose cells and thus are likely to be involved in the Gs and Gi mediated modulation of the glucose transport response to insulin as well . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| SNAP 23 is therefore likely to be a fusion catalyst along with syntaxin 4 and vesicle associated membrane protein ( VAMP ) 2 . ^^^ A measurable fraction of SNAP 23 was sedimented with cytoskeletal elements when extracted with Triton 10 100 , unlike VAMP 2 and syntaxin 4 , which were exclusively soluble in detergent . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Syntaxin 3 is present on the apical membrane and forms a complex with SNAP 23 which is also localized on the basolateral membrane where it forms a complex with syntaxin 4 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| An interaction of SNAP 23 and syntaxin 4 on the plasma membrane with vesicle associated synaptobrevin 2 and / or cellubrevin , known as SNAP ( soluble N ethyl maleimide sensitive factor attachment protein ) receptors or SNAREs , has been proposed to provide the targeting and / or fusion apparatus for insulin stimulated translocation of the GLUT 4 isoform of glucose transporter to the plasma membrane . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Synaptosome associated 23 kDa protein ( SNAP 23 ) is a widely expressed isoform of SNAP 25 , the principal t SNARE of neuronal cells , and colocalizes with syntaxin 4 in the plasma membrane of 3T3 L 1 adipocytes . ^^^ Wild type SNAP 23 ( SNAP 23 WT ) promoted the interaction between syntaxin 4 and VAMP 2 both in vitro and in vivo . ^^^ Although SNAP 23 DeltaC49 bound to neither syntaxin 4 nor VAMP 2 , the SNAP 23 DeltaC8 mutant bound to syntaxin 4 but not to VAMP 2 . ^^^ In addition , although SNAP 23 DeltaC8 bound to syntaxin 4 , it did not mediate the interaction between syntaxin 4 and VAMP 2 . ^^^ Together , these results demonstrate that SNAP 23 contributes to insulin dependent trafficking of GLUT 4 to the plasma membrane in 3T3 L 1 adipocytes by mediating the interaction between t SNARE ( syntaxin 4 ) and 5 SNARE ( VAMP 2 ) . . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Studies have identified key roles for vesicle associated membrane protein 2 ( VAMP 2 ) and syntaxin 4 in this event , and more recently have also implicated SNAP 23 and Munc18c in this process . ^^^ Such analyses suggest that in 3T3 L 1 adipocytes there are approximately 374 , 000 copies of syntaxin 4 , 1 . 15 10 10 ( 6 ) copies of SNAP 23 , 495 , 000 copies of VAMP 2 , 4 . 3 10 10 ( 6 ) copies of cellubrevin and 452 , 000 copies of Munc18c per cell , compared to previous estimates of 280 , 000 copies of Glut 4 . ^^^ Thus , the main SNARE proteins involved in insulin stimulated Glut 4 exocytosis ( syntaxin 4 and VAMP 2 ) are expressed in approximately equimolar amounts in adipocytes , whereas by contrast the endosomal 5 SNARE cellubrevin is present at approximately 10 fold higher levels and the t SNARE SNAP 23 is also present in an approximately 3 fold molar excess . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| In platelets , cellular activation by thrombin or phorbol 12 myristate 13 acetate decreased the binding of syntaxin 4 with SNAP 23 , another platelet t SNARE . ^^^ Phosphatase inhibitors increased syntaxin 4 phosphorylation and further decreased syntaxin 4 SNAP 23 binding induced by cell activation . ^^^ Conversely , a PKC inhibitor blocked syntaxin 4 phosphorylation and returned binding of syntaxin 4 SNAP 23 to that seen in nonstimulated platelets . ^^^ PKC phosphorylation in vitro inhibited ( 71 + / 8 % ) the binding of syntaxin 4 to SNAP 23 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Upon treatment of human fibroblasts with N ethyl maleimide , SNAP 23 dissociates from vimentin filaments and forms a protein complex with syntaxin 4 , a plasma membrane SNARE . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The t SNAREs syntaxin 4 and SNAP 23 were also abundant in these regions . ^^^ |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The phosphorylation of syntaxin 4 decreased its binding to its partner SNAP 23 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The SNARE isoforms VAMP 2 , SNAP 23 , and syntaxin 4 have been described in secretory processes in myeloid cells . ^^^ OBJECTIVE : We sought to determine whether eosinophils express the t SNARE isoforms SNAP 23 and syntaxin 4 as potential binding targets for VAMP 2 during exocytosis . ^^^ METHODS : Human peripheral blood eosinophils ( > 97 % ) from atopic subjects were subjected to RT PCR and sequence analysis by using specific primers for SNAP 23 and syntaxin 4 . ^^^ RESULTS : Nucleotide sequences obtained from PCR products exhibited nearly identical ( > 95 % ) homology with reported sequences for human SNAP 23 and syntaxin 4 . ^^^ Both SNAP 23 and syntaxin 4 were present in plasma membranes , with some staining in endoplasmic reticulum and Golgi membranes . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Although small , this population partially colocalizes with SNARE proteins SNAP 23 and syntaxin 4 . ^^^ |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| RT PCR and western blot analysis confirmed the presence of the SNARE homologs VAMP / synaptobrevin 2 , syntaxin 1 , syntaxin 4 and SNAP 23 in CD 8 cells . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| We also review recent findings showing that the candidate exocytotic SNAREs SNAP 23 and syntaxin 4 colocalize with SCAMPs , especially SCAMP 2 , and that SCAMP 2 is likely to be a partner in the compound exocytotic process . . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| We emphasize the role of soluble N ethylmaleimide attachment protein receptors ( SNAREs ) proteins such as syntaxin 4 that can promote membrane fusion through formation of a stable complex with SNAP 23 . ^^^ Associated kinase activity , which diminishes after stimulation as a consequence of intracellular calcium increases , specifically phosphorylates syntaxin 4 thereby affecting its capacity to bind to its t SNARE partner SNAP 23 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Glut 4 vesicle fusion is thought to be catalyzed by the interaction of the vesicle soluble N ethyl maleimide sensitive fusion protein attachment protein receptor VAMP 2 with the target soluble N ethyl maleimide sensitive fusion protein attachment protein receptors SNAP 23 and syntaxin 4 . ^^^ Here , we use combined membrane fractionation , detergent solubility , and sucrose gradient flotation to demonstrate that the large majority ( > 70 % ) of SNAP 23 and a significant proportion of syntaxin 4 ( approximately 35 % ) are associated with plasma membrane lipid rafts in 3T3 L 1 adipocytes . ^^^ Insulin stimulation had no effect on the plasma membrane raft association of SNAP 23 or syntaxin 4 but promoted VAMP 2 insertion into plasma membrane rafts . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| VAMP 2 , syntaxin 4 , and SNAP 23 are plausible candidate SNARE proteins for non neuronal exocytosis . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| In order to better understand the molecular mechanisms of platelet granule secretion , we evaluated the effect of activation induced degranulation on three functional platelet SNARE proteins , SNAP 23 , VAMP 3 , and syntaxin 4 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Analysis of syntaxin 4 and SNAP 23 , plasma membrane localized target receptor proteins ( t SNAREs ) for the GLUT 4 vesicle , showed that they were not cell surface targets of O linked glycosylation . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| This fusion event is mediated by SNARE proteins , syntaxin 4 , SNAP 23 and VAMP 2 , through a process regulated by accessory proteins whose roles are still unclear . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| SNAP 23 interacted with syntaxin 3 , syntaxin 4 , VAMP 2 , VAMP 3 and VAMP 8 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Tomosyn interacts with the t SNAREs syntaxin 4 and SNAP 23 and plays a role in insulin stimulated GLUT 4 translocation . ^^^ Tomosyn formed a high affinity ternary complex with Syntaxin 4 and SNAP 23 that was competitively inhibited by VAMP 2 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Finally , we show that Snapin is able to form a ternary complex with SNAP 23 and syntaxin 4 , suggesting that it is a component of non neuronal SNARE complexes . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| SNAP 23 mutants that mimic phosphorylation at Ser23 / Thr24 inhibited syntaxin 4 interactions , whereas a phosphorylation mutant of Ser 161 had only minor effects . ^^^ Because mutants that mimic SNAP 23 phosphorylation affect syntaxin 4 interactions , we hypothesize that SNAP 23 phosphorylation may be important for modulating SNARE complex interactions during membrane trafficking and fusion . . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| SNAREs were found either excluded ( syntaxin 2 ) , equally distributed between raft and non raft fractions ( syntaxin 4 , VAMP 8 , VAMP 2 ) , or selectively enriched in rafts ( syntaxin 3 , SNAP 23 ) . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Several `` soluble N ethylmaleimide sensitive fusion protein attachment protein receptor ' ' ( SNARE ) proteins have been identified in rat parotid acinar cells , including VAMP 2 , syntaxin 4 , and SNAP 23 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The amount and subcellular distribution of syntaxin 4 , SNAP 23 , Munc 18c , and GLUT 1 were unchanged in either aP 2 GLUT4 / or aP 2 GLUT4 Tg adipocytes , but transferrin receptor was partially redistributed to the plasma membrane in aP 2 GLUT4 Tg adipocytes . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| We had reported that in the rat pancreas , Munc18c co localizes with t SNAREs syntaxin 4 and SNAP 23 on the acinar cell basolateral plasma membrane . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Quantitative reverse transcription PCR ( RT PCR ) showed that mRNA levels of GLUT 4 , insulin receptor , insulin receptor substrate ( IRS ) 1 and IRS 2 , syntaxin 4 , SNAP 23 , and VAMP 2 in adipose tissues of BRS 3 deficient mice were unchanged compared with those in wild type control mice . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| VAMP 8 is enriched on the membrane of zymogen granules and exists in a complex with syntaxin 4 and SNAP 23 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| The fusion of Glut 4 containing vesicles at the plasma membrane is known to involve the target SNAREs syntaxin 4 and SNAP 23 and the vesicle SNARE VAMP 2 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Although triggering exocytosis did not alter the absolute amount of SNAP 23 bound to SNAREs , after stimulation essentially all of the SNAP 23 bound to the plasma membrane SNARE syntaxin 4 and the vesicle SNARE VAMP 2 was phosphorylated . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Fusion of the GLUT 4 containing vesicles with the plasma membrane is mediated by a soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) complex consisting of vesicle associated membrane protein 2 ( VAMP 2 ) , 23 kDa synaptosomal associated protein ( SNAP 23 ) , and syntaxin 4 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Cholesterol dependent syntaxin 4 and SNAP 23 clustering regulates caveolar fusion with the endothelial plasma membrane . ^^^ Imaging of PM sheets demonstrated and biochemical findings showed that the t SNARE proteins present in endothelial cells ( SNAP 23 and syntaxin 4 ) formed cholesterol dependent clusters in discrete areas of the PM . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Surface delivery of membrane carrying newly synthesized TNFalpha is controlled and limited by the level of soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) proteins syntaxin 4 and SNAP 23 . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| These cells did not express syntaxin 1A , vesicle associated membrane protein 1 ( VAMP 1 ) , or synaptosomal associated protein of 25 kDa ( SNAP 25 ) , but did express a family of SNARE proteins that has been implicated in glucose transporter trafficking , including syntaxin 4 , vesicle associated membrane protein 2 ( VAMP 2 ) , and synaptosomal associated protein of 23 kDa ( SNAP 23 ) . cDNAs encoding variants of SNAP 23 were co transfected with Myc tagged EAAC 1 to determine if SNAP 23 function was required for maintenance of EAAC 1 surface expression . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| Previous studies indicated that the 5 SNARE , vesicle associated membrane protein ( VAMP ) 2 , as well as t SNAREs ( SNAP 23 , syntaxin 4 and 6 ) are implicated in exocytosis from human granulocytes . ^^^ Syntaxin 4 , but not syntaxin 6 , was found to interact with SNAP 23 and was partially involved in mediator secretion from multiple compartments . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| In this study , we show that combinatorial soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) complexes with vesicle associated membrane proteins ( VAMPs ) , 23 kDa synaptosome associated protein ( SNAP 23 ) , and syntaxin 4 underlie the differential mobilization of granules in human neutrophils . ^^^ These data indicate that at least two SNARE complexes , made up of syntaxin 4 / SNAP 23 / VAMP 1 and syntaxin 4 / SNAP 23 / VAMP 2 , are involved in the exocytosis of specific and tertiary granules , whereas interactions between syntaxin 4 and VAMP 1 / VAMP 7 are involved in the exocytosis of azurophilic granules . ^^^ |
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| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and Q12846 |
Pubmed |
SVM Score :0.0 |
| NA |
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