| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.71220921 |
| CONCLUSIONS : The plasma membrane associated t SNAREs SNAP 23 and syntaxin 4 are expressed in human eosinophils and are likely candidates for association with VAMP 2 during docking , which is followed by exocytosis . 0.71220921^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Binary interactions of the SNARE proteins syntaxin 4 , SNAP 23 , and VAMP 2 and their regulation by phosphorylation . ^^^ In this study , we defined the binding characteristics of three SNARE proteins : SNAP 23 , VAMP 2 , and syntaxin 4 . ^^^ Binary , saturable interactions among all three partners ( VAMP 2 syntaxin 4 , VAMP 2 SNAP 23 , and SNAP 23 syntaxin 4 ) were measured in vitro . ^^^ Unlike its neuronal counterpart , SNAP 23 did not potentiate VAMP 2 binding to its putative t SNARE partner , syntaxin 4 . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting of intracellular vesicles immunoisolated using an aquaporin 2 antibody revealed the presence of both SNAP 23 and synaptobrevin 2 ( VAMP 2 ) in aquaporin 2 bearing vesicles . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| VAMP 2 and VAMP3 / cellubrevin ( 5 SNARE ' s ) have been shown to interact with the t SNARE ' s syntaxin 4 and SNAP 23 . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| A measurable fraction of SNAP 23 was sedimented with cytoskeletal elements when extracted with Triton 10 100 , unlike VAMP 2 and syntaxin 4 , which were exclusively soluble in detergent . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Protein kinase B stimulates the translocation of GLUT 4 but not GLUT 1 or transferrin receptors in 3T3 L 1 adipocytes by a pathway involving SNAP 23 , synaptobrevin 2 , and / or cellubrevin . ^^^ An interaction of SNAP 23 and syntaxin 4 on the plasma membrane with vesicle associated synaptobrevin 2 and / or cellubrevin , known as SNAP ( soluble N ethyl maleimide sensitive factor attachment protein ) receptors or SNAREs , has been proposed to provide the targeting and / or fusion apparatus for insulin stimulated translocation of the GLUT 4 isoform of glucose transporter to the plasma membrane . ^^^ By microinjecting 3T3 L 1 adipocytes with the Clostridium botulinum toxin B or E , which proteolyzed synaptobrevin 2 / cellubrevin and SNAP 23 , respectively , we investigated the role of these SNAREs in GLUT 4 , GLUT 1 , and transferrin receptor trafficking . ^^^ Thus , insulin appears to induce GLUT 4 translocation via two distinct routes , only one of which involves SNAP 23 and synaptobrevin 2 / cellubrevin , and can be mobilized by PKB DD . ^^^ The PKB , SNAP 23 , and synaptobrevin 2 / cellubrevin independent GLUT 4 translocation pathway may involve movement through recycling endosomes , together with GLUT 1 and transferrin receptors . . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| In vitro biochemical assays demonstrated that the syntaxin 2 variants exhibit similar yet distinct interactions with other proteins implicated in vesicular trafficking , including SNAP 25 , SNAP 23 , VAMP 2 and n sec 1 . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Wild type SNAP 23 ( SNAP 23 WT ) promoted the interaction between syntaxin 4 and VAMP 2 both in vitro and in vivo . ^^^ Although SNAP 23 DeltaC49 bound to neither syntaxin 4 nor VAMP 2 , the SNAP 23 DeltaC8 mutant bound to syntaxin 4 but not to VAMP 2 . ^^^ In addition , although SNAP 23 DeltaC8 bound to syntaxin 4 , it did not mediate the interaction between syntaxin 4 and VAMP 2 . ^^^ Together , these results demonstrate that SNAP 23 contributes to insulin dependent trafficking of GLUT 4 to the plasma membrane in 3T3 L 1 adipocytes by mediating the interaction between t SNARE ( syntaxin 4 ) and 5 SNARE ( VAMP 2 ) . . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| However , when vesicle associated membrane protein ( VAMP ) 2 was immunoprecipitated from lysates of parotid acinar cells , syntaxin 4 and SNAP 23 were not coprecipitated with VAMP 2 , although syntaxin 1 and SNAP 25 , t SNAREs in neuronal cells , were clearly coprecipitated with VAMP 2 from brain lysates . ^^^ Inversely , when syntaxin 4 was immunoprecipitated from parotid lysates , SNAP 23 , Munc18c , and N ethylmaleimide sensitive fusion protein ( NSF ) were coprecipitated , but VAMP 2 was again undetectable . ^^^ These results suggest that the interaction between syntaxin 4 , SNAP 23 and VAMP 2 is fairly weak and their concentrations in the cell lysate are insufficient to make a readily detectable complex , and that bindings between these proteins are hindered by other proteins in parotid acinar cells . . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Studies have identified key roles for vesicle associated membrane protein 2 ( VAMP 2 ) and syntaxin 4 in this event , and more recently have also implicated SNAP 23 and Munc18c in this process . ^^^ Such analyses suggest that in 3T3 L 1 adipocytes there are approximately 374 , 000 copies of syntaxin 4 , 1 . 15 10 10 ( 6 ) copies of SNAP 23 , 495 , 000 copies of VAMP 2 , 4 . 3 10 10 ( 6 ) copies of cellubrevin and 452 , 000 copies of Munc18c per cell , compared to previous estimates of 280 , 000 copies of Glut 4 . ^^^ Thus , the main SNARE proteins involved in insulin stimulated Glut 4 exocytosis ( syntaxin 4 and VAMP 2 ) are expressed in approximately equimolar amounts in adipocytes , whereas by contrast the endosomal 5 SNARE cellubrevin is present at approximately 10 fold higher levels and the t SNARE SNAP 23 is also present in an approximately 3 fold molar excess . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| RT PCR analysis showed the existence of SNARE proteins , including syntaxin 2 , 3 , 4 , SNAP 23 , VAMP 2 , and VAMP 7 . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Protein levels were compared with those of other molecules also implicated in organelle trafficking , including syntaxin 1 and vesicle associated membrane protein ( VAMP 2 ) and the nonneuronal analogues SNAP 23 and cellubrevin . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Third , antibody inhibition studies show a requirement for SNAP 25 , SNAP 23 , syntaxins 1A , 1B , 4 , and 6 , and VAMP 2 . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Feline oesophageal smooth muscle of all regions examined demonstrated the presence of SNAP 23 , SNAP 25 , syntaxins 1 to 4 , and VAMP 2 on the plasma membrane . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| An immunoblot analysis identified the astrocyte marker glial fibrillary acidic protein , microtubule associated protein 2 , the 5 SNAREs VAMP 1 , VAMP 2 , and cellubrevin and the t SNAREs syntaxin 1 , SNAP 23 , and SNAP 25 . 3 . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Glut 4 vesicle fusion is thought to be catalyzed by the interaction of the vesicle soluble N ethyl maleimide sensitive fusion protein attachment protein receptor VAMP 2 with the target soluble N ethyl maleimide sensitive fusion protein attachment protein receptors SNAP 23 and syntaxin 4 . ^^^ Insulin stimulation had no effect on the plasma membrane raft association of SNAP 23 or syntaxin 4 but promoted VAMP 2 insertion into plasma membrane rafts . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| VAMP 2 , syntaxin 4 , and SNAP 23 are plausible candidate SNARE proteins for non neuronal exocytosis . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| In conclusion , because all full length syntaxins examined bound VAMP 2 and SNAP 23 , but only non H 3 truncated syntaxin 1 bound H+ ATPase , and synt 1ADeltaC expression by intact IMCD cells inhibited H+ ATPase exocytosis , it is likely that the H+ ATPase binds directly to the H 3 domain of syntaxin 1 and not through VAMP 2 or SNAP 23 . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| This fusion event is mediated by SNARE proteins , syntaxin 4 , SNAP 23 and VAMP 2 , through a process regulated by accessory proteins whose roles are still unclear . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| SNAP 23 interacted with syntaxin 3 , syntaxin 4 , VAMP 2 , VAMP 3 and VAMP 8 . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Tomosyn formed a high affinity ternary complex with Syntaxin 4 and SNAP 23 that was competitively inhibited by VAMP 2 . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| SNAREs were found either excluded ( syntaxin 2 ) , equally distributed between raft and non raft fractions ( syntaxin 4 , VAMP 8 , VAMP 2 ) , or selectively enriched in rafts ( syntaxin 3 , SNAP 23 ) . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Several `` soluble N ethylmaleimide sensitive fusion protein attachment protein receptor ' ' ( SNARE ) proteins have been identified in rat parotid acinar cells , including VAMP 2 , syntaxin 4 , and SNAP 23 . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Collectively , our data suggest that 1 ) the A2b receptor is intracellular at rest ; 2 ) apical or basolateral agonist stimulation induces recruitment of the A2b receptor to the apical membrane ; 3 ) the SNARE proteins , VAMP 2 and SNAP 23 , participate in the recruitment of the A2b receptor ; and 4 ) the SNARE mediated recruitment of the A2b receptor may be required for its signaling . . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| The fusion of Glut 4 containing vesicles at the plasma membrane is known to involve the target SNAREs syntaxin 4 and SNAP 23 and the vesicle SNARE VAMP 2 . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Although triggering exocytosis did not alter the absolute amount of SNAP 23 bound to SNAREs , after stimulation essentially all of the SNAP 23 bound to the plasma membrane SNARE syntaxin 4 and the vesicle SNARE VAMP 2 was phosphorylated . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Fusion of the GLUT 4 containing vesicles with the plasma membrane is mediated by a soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) complex consisting of vesicle associated membrane protein 2 ( VAMP 2 ) , 23 kDa synaptosomal associated protein ( SNAP 23 ) , and syntaxin 4 . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Immunofluorescent localization of SNARE proteins in polarized mCCD epithelia detected the presence of syntaxins 1 and 3 and synaptosomal associated protein of 23 kDa ( SNAP 23 ) at the apical membrane , and vesicle associated membrane protein ( VAMP 2 ) was localized to intracellular compartments . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| The gliosomal fraction contained proteins of the exocytotic machinery [ syntaxin 1 , vesicular associated membrane protein type 2 ( VAMP 2 ) , 23 kDa synaptosome associated protein ( SNAP 23 ) and 25 kDa synaptosome associated protein ( SNAP 25 ) ] co existing with GFAP immunoreactivity . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| SNAP 23 , syntaxin 2 , and VAMP 2 were enriched in lipid rafts . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| These cells did not express syntaxin 1A , vesicle associated membrane protein 1 ( VAMP 1 ) , or synaptosomal associated protein of 25 kDa ( SNAP 25 ) , but did express a family of SNARE proteins that has been implicated in glucose transporter trafficking , including syntaxin 4 , vesicle associated membrane protein 2 ( VAMP 2 ) , and synaptosomal associated protein of 23 kDa ( SNAP 23 ) . cDNAs encoding variants of SNAP 23 were co transfected with Myc tagged EAAC 1 to determine if SNAP 23 function was required for maintenance of EAAC 1 surface expression . ^^^ |
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| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| Specific and tertiary granules contained VAMP 1 , VAMP 2 , and SNAP 23 , whereas the azurophilic granule membranes were enriched in VAMP 1 and VAMP 7 . ^^^ Ultrastructural , coimmunoprecipitation , and functional assays showed that SNARE complexes containing VAMP 1 , VAMP 2 , and SNAP 23 mediated the rapid exocytosis of specific / tertiary granules , whereas VAMP 1 and VAMP 7 mainly regulated the secretion of azurophilic granules . ^^^ These data indicate that at least two SNARE complexes , made up of syntaxin 4 / SNAP 23 / VAMP 1 and syntaxin 4 / SNAP 23 / VAMP 2 , are involved in the exocytosis of specific and tertiary granules , whereas interactions between syntaxin 4 and VAMP 1 / VAMP 7 are involved in the exocytosis of azurophilic granules . ^^^ |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00161 and P63027 |
Pubmed |
SVM Score :0.0 |
| NA |
|