| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.53124512 |
| Within cells , sgk 1 strongly associates with the microsomal fraction of homogenates and it colocalizes with the Na+ , K+ ATPase to the basolateral membrane . 0.53124512^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.79428113 |
| In addition , SGK 1 together with NHERF 2 stimulates the K+ channel ROMK 1 , suggesting a broader role of SGK 1 in regulation of ion transport . . 0.79428113^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| These data suggest that sgk plays a central role in aldosterone regulation of Na+ absorption and thus in the control of extracellular fluid volume , blood pressure , and sodium homeostasis . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Coexpression of the mouse sgk in Xenopus oocytes with the three subunits of the epithelial Na+ channel results in a significantly enhanced Na+ current . ^^^ These results suggest that sgk is an immediate early aldosterone induced gene , and this protein kinase plays an important role in the early phase of aldosterone stimulated Na+ transport . . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Starting at the transcriptional side of the aldosterone action , recent studies have identified the small G protein K Ras 2 and the kinase sgk as the first early aldosterone induced gene products potentially regulating Na+ transport . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Regulation of sgk by aldosterone and its effects on the epithelial Na ( + ) channel . ^^^ Recently , it has been reported that aldosterone induces the serum and glucocorticoid dependent kinase sgk and that coexpressing ENaC with this kinase in Xenopus laevis oocytes increases the amiloride sensitive Na ( + ) current ( Chen SY , Bhargava A , Mastroberardino L , Meijer OC , Wang J , Buse P , Firestone GL , Verrey F , and Pearce D . ^^^ Coexpressing sgk and ENaC in Xenopus oocytes evoked a fourfold increase in the amiloride blockable Na ( + ) channel activity . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| These data suggest that SGK is an important mediator of aldosterone effects on Na+ transport in tight epithelia . ^^^ In view of the existence of SGK homologues in invertebrates , it is interesting to speculate that SGK is an ancient kinase that was adapted to the control of epithelial Na+ transport by early vertebrates as they made the transition from a marine to a freshwater environment . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Expression of sgk resulted in a significant increase in the amiloride sensitive Na current , suggesting that this protein kinase plays an important role in the early phase of aldosterone stimulated Na transport . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| ENaC may contribute to the Na ( + ) channels activated by osmotic cell shrinkage in hepatocytes , whereby the relatively low amiloride and high EIPA sensitivity of the channel could at least be partially due to modification by SGK , which decreases the amiloride and increases the EIPA sensitivity of ENaC . . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| They do , however , inhibit the sgk induced increase in channel activity but only in oocytes that were preincubated in low Na ( + ) and had a high basal Na ( + ) current . ^^^ Thus mutating gammaT 630 or betaS 620 may limit the maximal channel activity achieved by a combination of sgk and low Na ( + ) . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Effects of the serine / threonine kinase SGK 1 on the epithelial Na ( + ) channel ( ENaC ) and CFTR : implications for cystic fibrosis . ^^^ The serine / threonine kinase SGK 1 has recently been shown to stimulate the activity of the epithelial Na ( + ) channel ENaC . ^^^ In addition CFTR mediated currents were also stimulated . h SGK 1 accelerated the expression of the amiloride sensitive Na ( + ) current in Xenopus oocytes paralleled by increased ENaC protein abundance in the oocyte membrane , an effect which was reversed by a h SGK 1 ( K127R ) mutation lacking the ATP binding site . ^^^ However , coexpression of h SGK 1 with ENaC altered the sensitivity of the Na ( + ) channel to the inhibitors amiloride and triamterene . ^^^ In summary , enhanced expression of h SGK 1 in epithelial cells of CF lung tissue may be a novel pathophysiological factor contributing to increased Na ( + ) channel activity and thus to increased Na ( + ) transport in CF . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Hence , SGK 1 might integrate the effects of multiple hormonal and nonhormonal regulators of Na ( + ) transport in tight epithelia and thereby play a key role in volume homeostasis . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Here we show that SGK and human Nedd 4 2 ( hNedd 4 2 ) converge in a common pathway to regulate epithelial Na+ absorption . ^^^ SGK phosphorylated hNedd 4 2 ( but not hNedd 4 ) , altering hNedd 4 2 function ; phosphorylation reduced the binding of hNedd 4 2 to alphaENaC , and hence , the hNedd 4 2 mediated inhibition of Na+ absorption . ^^^ These data suggest that SGK regulates epithelial Na+ absorption in part by modulating the function of hNedd 4 2 . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of Nedd 4 2 by Sgk 1 regulates epithelial Na ( + ) channel cell surface expression . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Absorption of NaCl is a rather steady process that is controlled by steroid hormones regulating the expression of epithelial Na ( + ) channels ( ENaC ) , the Na ( + ) K ( + ) ATPase , and additional modulating factors such as the serum and glucocorticoid regulated kinase SGK . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| To study the role of sgk ( serum , glucocorticoid induced kinase ) in hormonal regulation of Na+ transport mediated by the epithelial Na+ channel ( ENaC ) , clonal cell lines stably expressing human sgk , an S422A sgk mutant , or a D222A sgk mutant were created in the background of the A 6 model renal epithelial cell line . ^^^ Transfection of a S422A mutant form of sgk , which can not be phosphorylated by phosphatidylinositol dependent kinase ( PDK ) 2 , results in a cell line that is indistinguishable from the parent line in basal and hormone stimulated Na+ transport . ^^^ The D222A sgk mutant , which lacks kinase activity , functions as a dominant negative mutant inhibiting basal as well as peptide and steroid hormone stimulated Na+ transport . ^^^ Thus sgk activity is necessary for ENaC mediated Na+ transport . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Glucocorticoid stimulated lung epithelial Na ( + ) transport is associated with regulated ENaC and sgk 1 expression . ^^^ To understand its molecular basis , we examined the effect of glucocorticoids ( GC ) on epithelial Na ( + ) channel ( ENaC ) alpha , beta , and gamma and sgk 1 expression and determined the biophysical properties of Na ( + ) channels in these cells . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Hypotonic induction of SGK 1 and Na+ transport in A 6 cells . ^^^ In the distal nephron , SGK 1 is induced by aldosterone and regulates epithelial Na+ channel mediated transepithelial Na+ transport . ^^^ In other tissues , including liver and shark rectal gland , SGK 1 is regulated by hypertonic stress and is thought to modulate epithelial Na+ channel and Na+ K+ 2Cl cotransporter mediated Na+ transport . ^^^ In this report , we examined the regulation of SGK 1 mRNA and protein expression and Na+ currents in response to osmotic stress in A 6 cells , a cultured cell line derived from Xenopus laevis distal nephron . ^^^ We found that in contrast to hepatocytes and rectal gland cells , hypotonic conditions stimulated SGK 1 expression and Na+ transport in A 6 cells . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Stimulation of Xenopus oocyte Na ( + ) , K ( + ) ATPase by the serum and glucocorticoid dependent kinase sgk 1 . ^^^ Indeed , co expression of sgk 1 with the renal epithelial Na ( + ) channel ( ENaC ) augments Na ( + ) channel activity . ^^^ The aim of the present study was to examine possible effects of sgk 1 on Na ( + ) / K ( + ) ATPase activity . ^^^ The present results thus point to an additional action of sgk 1 that may participate in the regulation of renal tubular Na ( + ) transport . ^^^ Moreover , sgk 1 may be involved in the regulation of Na ( + ) / K ( + ) ATPase in extrarenal tissues . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The utilization of sophisticated molecular biology techniques led to the identification of genes encoding major components of the aldosterone regulated sodium reabsorptive pathway , such as mineralocorticoid receptor . 11b hydroxysteroid dehydrogenase type 2 , epithelia Na+ channel , and sgk and Kras genes . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The serum and glucocorticoid regulated kinase ( Sgk 1 ) involved in the aldosterone dependent signaling cascade enhances 1 ( Na ) by 2 . 5 fold ( P < 0 . 001 ) and N by 1 . 6 fold ( P < 0 . 001 ) , indicating a dual effect on N and wcP ( o ) . ^^^ Compared with Sgk 1 alone , coexpression of Sgk 1 with mCAP 1 leads to a ninefold increase in 1 ( Na ) ( P < 0 . 001 ) and 1 . 3 fold in N ( P < 0 . 02 ) . ^^^ The synergism between CAPs and Sgk 1 on 1 ( Na ) was always more than additive , indicating a true potentiation . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Concerted action of ENaC , Nedd 4 2 , and Sgk 1 in transepithelial Na ( + ) transport . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The serum and glucose regulated kinase ( SGK 1 ) gene has recently been identified as an important aldosterone induced protein kinase that mediates trafficking of the renal epithelial Na ( + ) channel ( ENaC ) to the cell membrane . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| In lung and collecting duct epithelia , glucocorticoid ( GC ) stimulated Na+ transport is preceded by an increase in the protein kinase sgk 1 , which in turn regulates the activity of the epithelial Na+ channel ( ENaC ) . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Impaired renal Na ( + ) retention in the sgk 1 knockout mouse . ^^^ The serum and glucocorticoid regulated kinase ( sgk 1 ) is induced by mineralocorticoids and , in turn , upregulates heterologously expressed renal epithelial Na ( + ) channel ( ENaC ) activity in Xenopus oocytes . ^^^ In contrast , dietary NaCl restriction reveals an impaired ability of sgk 1 ( / ) mice to adequately decrease Na ( + ) excretion despite increases in plasma aldosterone levels and proximal tubular Na ( + ) and fluid reabsorption , as well as decreases in blood pressure and glomerular filtration rate . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Hormone regulated transepithelial Na+ transport in mammalian CCD cells requires SGK 1 expression . ^^^ To study the role of serum and glucocorticoid inducible kinase 1 ( SGK 1 ) in mammalian cells , we compared Na ( + ) transport rates in wild type ( WT ) M 1 cortical collecting duct cells with M 1 populations stably expressing human full length SGK 1 , NH ( 2 ) terminal truncated ( DeltaN 60 ) SGK 1 , `` kinase dead ' ' ( K127M ) SGK 1 , and cells that have downregulated levels of SGK 1 mRNA ( antisense SGK 1 ) . ^^^ Basal rates of transepithelial Na ( + ) transport were highest in full length SGK 1 populations , compared among the above populations . ^^^ Dexamethasone treatment increased Na ( + ) transport in WT and full length SGK 1 cells 2 . 7 and 2 fold , respectively . ^^^ Modest stimulation of Na ( + ) absorption was detected after dexamethasone treatment in DeltaN 60 SGK 1 populations . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The serum and glucocorticoid inducible kinase SGK 1 and the Na+ / H+ exchange regulating factor NHERF 2 synergize to stimulate the renal outer medullary K+ channel ROMK 1 . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND / AIM : Expression of the constitutively active form of serum and glucocorticoid dependent kinase ( ( S422D ) SGK 1 ) in Xenopus oocytes has recently been shown to upregulate endogenous Na ( + ) / K ( + ) ATPase activity , an effect presumably participating in the regulation of cellular K ( + ) uptake and transepithelial Na ( + ) transport . ^^^ SGK 1 and the two isoforms SGK 2 and SGK 3 are stimulated by insulin and insulin like growth factor 1 ( IGF 1 ) , which have been shown to enhance Na ( + ) / K ( + ) ATPase activity in a variety of cells . ^^^ The present experiments have been performed to elucidate whether or not wild type SGK 1 , SGK 2 and SGK 3 are similar to ( S422D ) SGK 1 in being effective regulators of Na ( + ) / K ( + ) ATPase . ^^^ CONCLUSION : The stimulating effect of SGK 1 on the Xenopus oocyte Na ( + ) / K ( + ) ATPase is mimicked by the isoforms SGK 2 and SGK 3 . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The serine / threonine kinases SGK 2 and SGK 3 are potent stimulators of the epithelial Na+ channel alpha , beta , gamma ENaC . ^^^ The serum and glucocorticoid inducible kinase 1 ( SGK 1 ) has been identified as a signalling molecule up regulated by aldosterone , which stimulates the renal epithelial Na ( + ) channel ENaC . ^^^ Dual electrode voltage clamp experiments in Xenopus laevis oocytes expressing alpha , ss , gamma ENaC with or without SGK 1 , SGK 2 or SGK 3 revealed a stimulatory effect of all three kinases on the amiloride sensitive current ( 1 ( Na ) ) . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| SGK 1 : aldosterone induced relay of Na+ transport regulation in distal kidney nephron cells . ^^^ Cell surface localization and function of ENaC and of the Na , K ATPase are also tightly controlled by a complex regulatory network and aldosterone appears to acutely regulate the expression of elements of this network such as the small G protein K Ras ( in A 6 cells ) and the kinase SGK 1 ( also in ENaC expressing cells of the mammalian distal nephron ) . ^^^ Active SGK 1 has been shown to increase ENaC and Na , K ATPase cell surface expression in Xenopus oocytes . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The serum and glucocorticoid inducible kinase SGK 1 stimulates the Na+ channels ENaC and SCN5A , the K+ channels ROMK 1 , Kv1 . 3 , and KCNE1 / KCNQ1 , the cation conductance induced by 4F2 / LAT1 and the chloride conductance induced by CFTR . ^^^ An in vivo role of SGK 1 mediated ENaC channel regulation in renal salt excretion and blood pressure control is documented by the impaired ability of SGK 1 knockout mice to adequately reduce renal Na+ output and maintain blood pressure during dietary salt restriction and by enhanced blood pressure in individuals carrying certain polymorphisms in the SGK 1 gene . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| One of the functions of SGK 1 is the phosphorylation and inactivation of the ubiquitin ligase Nedd 4 2 , which in turn could be shown to downregulate the voltage gated Na+ channel SCN5A ( hH 1 ) . ^^^ To this end cRNA encoding the human Na+ channel SCN5A was injected into Xenopus laevis oocytes with or without cRNA encoding the wild type kinases SGK 1 , the constitutively active kinase ( S422D ) SGK 1 , the inactive form K127NSGK1 or the wild type SGK 3 . ^^^ The present observations disclose a role of the kinases SGK 1 and SGK 3 in the regulation of cardiac Na+ channels . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Mineralocorticoid effects in the kidney : correlation between alphaENaC , GILZ , and Sgk 1 mRNA expression and urinary excretion of Na+ and K+ . ^^^ This study measured the Na ( + ) and K ( + ) urinary excretion and the mRNA levels of three known aldosterone induced transcripts , the serum and glucocorticoid induced kinase ( Sgk 1 ) , the alpha subunit of the epithelial Na ( + ) channel ( alphaENaC ) , and the glucocorticoid induced leucine zipper protein ( GILZ ) in the whole kidney and in isolated cortical collecting tubules of adrenalectomized rats treated with low doses of aldosterone and / or dexamethasone . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Short term aldosterone action on Na , K ATPase surface expression : role of aldosterone induced SGK 1 . ^^^ Long term effects of aldosterone ( starting within 3 to 6 hours and increasing over days ) are mediated by the direct and indirect induction of stable elements of the Na ( + ) transport machinery ( e . g . , Na , K ATPase alpha subunit ) , whereas short term effects appear to be mediated by the upregulation of short lived elements of the machinery ( e . g . , ENaC alpha subunit ) and of regulatory proteins , such as the serum and glucocorticoid regulated kinase SGK 1 . ^^^ As a first approach to address the question whether the aldosterone induced regulatory protein SGK 1 might play a role in mediating Na , K ATPase translocation , we have used the Xenopus laevis expression system . ^^^ SGK 1 coexpression indeed increased both the Na ( + ) pump current and the surface expression of pumps containing the rat alpha 1 subunits . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The renal outer medullary K ( + ) channel ROMK 1 is upregulated by the serum and glucocorticoid inducible kinase SGK 1 , an effect potentiated by Na ( + ) / H ( + ) exchanger regulating factor NHERF 2 . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The serum and glucocorticoid inducible kinase SGK 1 stimulates the renal outer medullary K ( + ) channel ROMK 1 in the presence of the Na ( + ) / H ( + ) exchanger regulating factor NHERF 2 . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Trafficking of the Na ( + ) / H ( + ) exchanger NHE 3 is controlled by NHE regulating factors NHERF 1 and NHERF 2 and the serum and glucocorticoid inducible kinase SGK 1 . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| SGK 1 increases Na , K ATP cell surface expression and function in Xenopus laevis oocytes . ^^^ To address the question of whether aldosterone induced serum and glucocorticoid regulated kinase 1 ( SGK 1 ) might be involved in mediating this regulation of Na , K ATPase subcellular localization , similar to that of the epithelial Na ( + ) channel ( ENaC ) , we co expressed the Na , K ATPase ( rat alpha 1 and Xenopus laevis beta 1 subunits ) and Xenopus SGK 1 in Xenopus oocytes . ^^^ Measurements of the Na ( + ) pump current showed that wild type SGK 1 increases the function of exogenous Na , K ATPase at the surface of Xenopus oocytes . ^^^ In contrast , the functional surface expression of two other exogenous transporters , the heterodimeric amino acid transporter LAT 1 4F2hc and the Na ( + ) / phosphate cotransporter NaPi IIa , was not increased by SGK 1 co expression . ^^^ The total pool of exogenous Na , K ATPase was increased by the co expression of SGK 1 , and similarly also by ENaC co expression . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The current study demonstrates that aldosterone increases Na ( + ) transport , in part , by stimulating PI 3 K signaling and that during aldosterone actions , there is both signaling convergence between the two aldosterone induced proteins , K RasA and Sgk , as well as cross talk between the PI 3 K and MAPK1 / 2 cascades with the prior but not latter cascade enhancing ENaC activity . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| In Xenopus laevis oocytes , SGK 1 stimulates the epithelial Na ( + ) channel by phosphorylating the ubiquitin ligase Nedd 4 2 , which regulates channels by ubiquitination leading to subsequent degradation of the channel protein . ^^^ Thus the present study has been performed to explore whether SGK 1 regulates transport systems expressed in intestinal epithelial cells , specifically type IIb sodium phosphate ( Na ( + ) P ( 1 ) ) cotransporter ( NaPi IIb ) . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The serum and glucocorticoid inducible kinase ( SGK 1 ) is well known to up regulate the renal epithelial Na ( + ) channel ENaC . ^^^ Excessive SGK 1 activity would be expected to cause renal Na ( + ) retention and blood pressure increase . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| This review briefly summarizes two areas of mineralocorticoid research : molecular determinants of MR specificity , and the role of Sgk 1 in mediating the effects of aldosterone on epithelial Na ( + ) transport . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The present study explores whether SGK 1 is expressed in the retina and whether it regulates EAAT 3 , a Na ( + ) coupled glutamate transporter . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| We studied the role of SGK 1 in corticosteroid regulated Na transport in M 1 mouse CCD cell lines that stably over express or down regulate SGK 1 . ^^^ Basal rates of transepithelial Na transport were significantly lower in CCD cells in which SGK 1 expression or activity was down regulated than in SGK 1 overexpressing cells . ^^^ Importantly , corticosteroid treatment failed to stimulate Na transport in cells with down regulated SGK 1 while it significantly increased Na transport in parent and SGK 1 overexpressing M 1 cells . ^^^ We conclude that SGK 1 is a critical component in corticosteroid regulated Na transport in mammalian CCD cells . ^^^ Our data also indicate that the N terminal of SGK 1 is necessary for its stimulatory effect on Na transport while elimination of the C terminal PDZ binding domain did not change its function . . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Both the phosphorylation level and the accompanying amiloride sensitive Na ( + ) currents could be further enhanced to approximately the same levels by coexpressing Sgk 1 . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| AMP stimulated Na+ transport in H 441 distal lung epithelial cells : role of PKA , phosphatidylinositol 3 kinase , and sgk 1 . ^^^ To understand the molecular basis for the stimulation of Na+ transport , we delineated the role of specific intracellular pathways and examined the effect of cAMP on alphabetagamma epithelial Na+ channel ( ENaC ) and sgk 1 expression . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| SGK regulates cell volume of various cells by effects on Na ( + ) and K ( + ) transport through membrane channels . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The serum and glucocorticoid regulated kinase ( SGK 1 ) gene is an important mediator of aldosterone action , regulating the expression of the renal epithelial Na ( + ) channel . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| AMP and serum and glucocorticoid inducible kinase ( SGK ) regulate the epithelial Na ( + ) channel through convergent phosphorylation of Nedd 4 2 . ^^^ Previous work found that these residues are also phosphorylated by serum and glucocorticoid inducible kinase ( SGK ) , a downstream mediator by which aldosterone regulates epithelial Na ( + ) transport . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Serum and glucocorticoid regulated kinase ( SGK ) plays a key role in the regulation of epithelial Na+ transport . ^^^ SGK phosphorylates Nedd 4 2 , an E 3 ubiquitin protein ligase that targets the epithelial Na+ channel ( ENaC ) for degradation . ^^^ Thus , SGK expression must be tightly controlled to maintain Na+ homeostasis . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| PURPOSE OF REVIEW : The serum and glucocorticoid inducible kinase 1 ( SGK 1 ) is induced in the aldosterone sensitive distal nephron ( ASDN ) where it may stimulate Na reabsorption , partly by inhibiting ubiquitin ligase Nedd 4 2 mediated retrieval of epithelial Na+ channel ENaC from the luminal membrane . ^^^ SGK 1 dependent upregulation of Na+ reabsorption in ASDN contributes to upregulation of renal K+ excretion . ^^^ In oocytes , SGK 1 activates various renal transport proteins including Na+ / glucose cotransporter SGLT 1 , Na+ coupled dicarboxylate transporter NaDC 1 , epithelial Ca+ channel TRPV 5 , renal outer medullary K+ channel ROMK and voltage gated K+ channels KCNE1 / KCNQ1 and Kv1 . 3 . ^^^ SUMMARY : PPAR gamma activators may increase renal Na reabsorption by stimulating SGK 1 and ENaC . ^^^ K+ homeostasis requires SGK 1 dependent Na+ reabsorption in ASDN . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The studies indicate that Sgk 1 is not absolutely required for Na ( + ) reabsorption and K ( + ) secretion in the ASDN . ^^^ This may involve Sgk 1 mediated stimulation of basolateral Na ( + ) K ( + ) ATPase as well as retention of epithelial Na ( + ) channel , ENaC , in the apical membrane . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| We found that this interaction preserved the activity of the Sgk 1 stimulated ENaC dependent Na+ current while disrupting the interaction decreased ENaC density on the Xenopus laevis oocytes surface possibly by enhancing Nedd 4 2 mediated ubiquitination that leads to ENaC degradation . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Role of SGK 1 in nitric oxide inhibition of ENaC in Na+ transporting epithelia . ^^^ Our study also identified iNOS as a novel SGK 1 phosphorylated protein ( at S 733 and S 903 residues in miNOS ) suggesting that one way in which SGK 1 could increase Na ( + ) transport is by altering iNOS production of NO . . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| This augmentation , which is accompanied by a raise in Sgk 1 expression and transepithelial Na+ transport , is sensitive to phosphatidylinositol 3 kinase inhibition , as is Sgk 1 phosphorylation and Na+ transport . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The second common gene variant ( prevalence , 3 % 5 % in unselected Caucasians ) , to be discussed in more detail , affects the serum and glucocorticoid inducible kinase SGK 1 , a kinase upregulated by mineralocorticoids and enhancing the activity of ENaC , ROMK , and Na+ / K+ATPase . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The treatment enhanced renal Na ( + ) excretion significantly more in sgk 1 ( + / + ) mice ( from 3 + / 1 to 134 + / 32 micromol . 24 h ( 1 ) . g body wt ( 1 ) ) than in sgk 1 ( / ) mice ( from 4 + / 1 to 49 + / 8 micromol . 24 h ( 1 ) . g body wt ( 1 ) ) , pointing to SGK 1 dependent stimulation of salt intake . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The concerted action of SGK 1 and 14 3 3 appears to disrupt Nedd 4 2 mediated ubiquitination of ENaC , thus providing a mechanism by which SGK 1 modulates the ENaC mediated Na ( + ) current . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| We investigated the effect of MPA on classic mineralocorticoid target genes , alpha epithelial Na channel ( ENaC ) and sgk 1 , in the collecting duct . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| SGK 1 activates Na+ K+ ATPase in amphibian renal epithelial cells . ^^^ Serum and glucocorticoid induced kinase 1 ( SGK 1 ) is thought to be an important regulator of Na ( + ) reabsorption in the kidney . ^^^ It has been proposed that SGK 1 mediates the effects of aldosterone on transepithelial Na ( + ) transport . ^^^ Previous studies have shown that SGK 1 increases Na ( + ) transport and epithelial Na ( + ) channel ( ENaC ) activity in the apical membrane of renal epithelial cells . ^^^ SGK 1 has also been implicated in the modulation of Na ( + ) K ( + ) ATPase activity , the transporter responsible for basolateral Na ( + ) efflux , although this observation has not been confirmed in renal epithelial cells . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Under control diet , fluid intake , urinary flow rate , urinary Na ( + ) , K ( + ) , and Cl ( ) excretion , and blood pressure were similar in sgk 1 ( / ) and sgk 1 ( + / + ) mice . ^^^ Additional high NaCl diet ( 4 % NaCl ) did not significantly alter fluid intake and urine volume but markedly increased urinary output of Na ( + ) and Cl ( ) , approaching values significantly ( P < 0 . 05 ) larger in sgk 1 ( / ) than in sgk 1 ( + / + ) mice ( Na ( + ) : 2 , 572 + / 462 vs . 1 , 428 + / 236 ; Cl ( ) : 2 , 364 + / 388 vs . 1 , 379 + / 225 micromol / 24 h ) . ^^^ |
|
| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| In vitro experiments have revealed the ability of serum and glucocorticoid inducible kinase 1 ( SGK 1 ) to stimulate intestinal Na ( + ) coupled glucose cotransporter 1 ( SGLT 1 ) and intestinal Na ( + ) / H ( + ) exchanger 3 ( NHE 3 ) . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The mechanism of aldosterone has been reported to elevate renal Na+ reabsorption by stimulating expression of epithelial Na+ channel ( ENaC ) and also activate an ENaC regulating protein kinase , serum and glucocorticoid regulated kinase 1 ( SGK 1 ) . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Although Sgk 1 is a cell survival kinase , its primary role likely concerns the regulation of epithelial ion transport , as suggested by the phenotype of Sgk 1 null mice , which display a defect in Na ( homeostasis owing to disturbed renal tubular Na+ handling . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The early natriuresis results from decreased Na ( + ) reabsorption in the PT and Sgk 1 related decreased ENaC activity in the DT and CD . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Serum and glucocorticoid inducible kinase ( SGK ) 1 and SGK 3 share the ability to upregulate several ion channels , including the epithelial Na ( + ) channel . ^^^ During salt depletion , absolute and fractional excretions of Na ( + ) were significantly ( P < 0 . 01 ) higher in sgk 1 ( / ) / sgk3 ( / ) ( 1 . 2 + / 0 . 2 micromol / 24 h g body wt , 0 . 12 + / 0 . 03 % ) than in sgk 1 ( + / + ) / sgk3 ( + / + ) ( 0 . 4 + / 0 . 1 micromol / 24 h g body wt , 0 . 04 + / 0 . 01 % ) mice . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| SGK 1 contributes to Na+ retention and K+ elimination of the kidney , mineralocorticoid stimulation of salt appetite , glucocorticoid stimulation of intestinal Na+ / H+ exchanger and nutrient transport , insulin dependent salt sensitivity of blood pressure and salt sensitivity of peripheral glucose uptake , memory consolidation , and cardiac repolarization . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| The roles of SGK 1 and NHERF 2 . ^^^ We identified serum and glucocorticoid induced protein kinase , SGK 1 , as the protein interacting with PDZ domains of NHERF 2 to regulate NHE 3 activity . ^^^ These data demonstrated that glucocorticoid activation of NHE 3 requires the activation of SGK 1 and the presence of NHERF 2 acting as a scaffold protein . . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Serum and glucocorticoid induced protein kinase 1 ( SGK 1 ) also carries a putative PDZ binding motif ( D S F L ) at its carboxy tail , implicated in the specific interaction with NHERF 2 . ^^^ Using pull down assays and co transfection experiments , we demonstrated that the DSFL tail of SGK 1 interacts with the first PDZ domain of NHERF 2 and the PIF of NHERF 2 binds to the PIF binding pocket of PDK 1 to form an SGK 1 NHERF2 PDK 1 complex . ^^^ Thus , it was suggested that NHERF 2 mediates the activation and phosphorylation of SGK 1 by PDK 1 through its first PDZ domain and PIF motif , as a novel SGK 1 activation mechanism . . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Regulation of the epithelial Ca2+ channel TRPV 5 by the NHE regulating factor NHERF 2 and the serum and glucocorticoid inducible kinase isoforms SGK 1 and SGK 3 expressed in Xenopus oocytes . ^^^ To this end cRNA encoding TRPV 5 has been injected into Xenopus oocytes with or without additional injection of SGK 1 , its isoforms SGK 2 and SGK 3 , constitutively active ( S422D ) SGK 1 , inactive ( K127N ) SGK 1 , constitutively active ( T308D , S473D ) PKB and / or the Na+ / H+ exchanger regulating factor NHERF 2 . ^^^ Coexpression of TRPV 5 with both ( S422D ) SGK 1 and NHERF 2 stimulates tracer Ca2+ entry , ICa and ICl ( Ca ) . ^^^ The effect of ( S422D ) SGK 1 on TRPV 5 and NHERF 2 expressing oocytes is mimicked by SGK 1 and SGK 3 , but not by SGK 2 , constitutively active ( T308D , S473D ) PKB or inactive ( K127N ) SGK 1 . ^^^ The observations suggest that SGK 1 , SGK 3 and NHERF 2 regulate TRPV 5 and are thus likely to participate in the regulation of calcium homeostasis . . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| Requirement of PDZ domains for the stimulation of the epithelial Ca2+ channel TRPV 5 by the NHE regulating factor NHERF 2 and the serum and glucocorticoid inducible kinase SGK 1 . ^^^ The present study aims to define the molecular requirements for the interaction of TRPV 5 with SGK 1 and NHERF 2 . ^^^ The experiments demonstrate the significance of SGK 1 and NHERF 2 as TRPV 5 modulators which are likely to participate in the regulation of calcium homeostasis by 1 , 25 ( OH ) 2D3 . . ^^^ |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15599 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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