| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| Although protein kinase A dependent phosphorylation of one of the three phospho acceptors in Kir1 . 1 , Ser 44 , also a canonical serum glucocorticoid regulated kinase ( SGK 1 ) phosphorylation site , controls the number of active channels , it is unknown whether this involves activating dormant channels already residing on the plasma membrane or recruiting new channels to the cell surface . ^^^ In conclusion , SGK 1 phosphorylation of Kir1 . 1 drives expression on the plasmalemma . ^^^ Cell surface expression of the ROMK ( Kir 1 . 1 ) channel is regulated by the aldosterone induced kinase , SGK 1 , and protein kinase A . ^^^ Here we explore the mechanism and test whether SGK 1 phosphorylation of ROMK regulates cell surface expression . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| Electrophysiological and immunohistochemical studies under high K+ diet indicated that reduced epithelial Na+ channel ENaC and / or Na+ / K+ ATPase activity in the aldosterone sensitive distal nephron accounted for the impaired response in sgk 1 / and that an enhanced apical abundance of renal outer medullary K+ channel ROMK partly compensated for the defect . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| In oocytes , SGK 1 activates various renal transport proteins including Na+ / glucose cotransporter SGLT 1 , Na+ coupled dicarboxylate transporter NaDC 1 , epithelial Ca+ channel TRPV 5 , renal outer medullary K+ channel ROMK and voltage gated K+ channels KCNE1 / KCNQ1 and Kv1 . 3 . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| The second common gene variant ( prevalence , 3 % 5 % in unselected Caucasians ) , to be discussed in more detail , affects the serum and glucocorticoid inducible kinase SGK 1 , a kinase upregulated by mineralocorticoids and enhancing the activity of ENaC , ROMK , and Na+ / K+ATPase . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| The expression of ROMK channels in the plasma membrane is regulated by protein tyrosine kinase ( PTK ) , serum and glucorticoid induced kinase ( SGK ) , and with no lysine kinase 4 . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| The serum and glucocorticoid inducible kinase SGK 1 and the Na+ / H+ exchange regulating factor NHERF 2 synergize to stimulate the renal outer medullary K+ channel ROMK 1 . ^^^ With the same method , ROMK 1 appeared to be insensitive to regulation by SGK 1 . ^^^ The present study has been performed to test whether NHERF 2 is required for regulation of ROMK 1 by SGK 1 . ^^^ Coexpression of neither NHERF 2 nor SGK 1 with ROMK 1 increases ROMK 1 activity . ^^^ However , coexpression of NHERF 2 and SGK 1 together with ROMK 1 markedly increases K ( + ) channel activity . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| In addition , SGK 1 together with NHERF 2 stimulates the K+ channel ROMK 1 , suggesting a broader role of SGK 1 in regulation of ion transport . . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| The serum and glucocorticoid inducible kinase SGK 1 stimulates the Na+ channels ENaC and SCN5A , the K+ channels ROMK 1 , Kv1 . 3 , and KCNE1 / KCNQ1 , the cation conductance induced by 4F2 / LAT1 and the chloride conductance induced by CFTR . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| Negative charge at the consensus sequence for the serum and glucocorticoid inducible kinase , SGK 1 , determines pH sensitivity of the renal outer medullary K+ channel , ROMK 1 . ^^^ The renal outer medullary K ( + ) channel ROMK 1 is upregulated by the serum and glucocorticoid inducible kinase SGK 1 , an effect potentiated by Na ( + ) / H ( + ) exchanger regulating factor NHERF 2 . ^^^ SGK 1 phosphorylates ROMK 1 at serine 44 . ^^^ To explore the role of SGK 1 phosphorylation , serine 44 was replaced by an alanine ( [ S44A ] ROMK 1 ) or an aspartate ( [ S44D ] ROMK 1 ) . ^^^ Wild type ROMK 1 , [ S44A ] ROMK 1 , and [ S44D ] ROMK 1 were expressed in Xenopus oocytes with or without constitutively active [ S422D ] SGK 1 and NHERF 2 , and K ( + ) current ( 1 ( KR ) ) determined . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| Molecular requirements for the regulation of the renal outer medullary K ( + ) channel ROMK 1 by the serum and glucocorticoid inducible kinase SGK 1 . ^^^ The serum and glucocorticoid inducible kinase SGK 1 stimulates the renal outer medullary K ( + ) channel ROMK 1 in the presence of the Na ( + ) / H ( + ) exchanger regulating factor NHERF 2 . ^^^ According to chemiluminescence and electrophysiology , deletion of the second PDZ domain of NHERF 2 or the putative PDZ binding motif on ROMK 1 abrogates the stimulating effect of SGK 1 on ROMK 1 protein abundance in the plasma membrane and K ( + ) current . . ^^^ |
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| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and O00141 |
Pubmed |
SVM Score :0.0 |
| NA |
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